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        Standard Power Pack P25 Manual
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1.   losses resulting from insufficient decontamination of the  device     e Please prepare written confirmation  use the    Equip   ment Decontamination Declaration    following on the next  page  that the device is free of biologically dangerous   chemical or radioactive contaminants  This confirmation  must be attached to the outside of the packaging     e Use the original packing or a similarly robust packing when  returning the device  If not available  contact Biometra or  your local distributor     e Label the outside of the box with    CAUTION  SENSITIVE  INSTRUMENT     and the RAN number sticker  Attach the  Decontamination Declarartion     e Please enclose a note which contains the following   a  Sender   s name and address   b  Name of a contact person for further inquiries with  telephone number   c  Precise description of the fault  which also reveals  during which procedures the fault occurred  if possible     19    Biometra    An Analytik Jena Company             9 0 Equipment Decontamination Certificate       To enable us to comply with german law  i e    71 StrISchV    17 GefStoffV  and   19 ChemG  and to avoid exposure to hazardous   materials during handling or repair  please complete this form  prior   to the equipment leaving your laboratory     COMPANY   INSTITUTE                   ADDRESS   PHONE NO FAX NO   E MAIL   EQUIPMENT Model Serial No   If on loan   evaluation Start Date  Finish Date    Hazardous materials used with this equipment              Meth
2.   pP green  No error   Power on          pP dark Error    e No connection to power  e Faulty fuse   e Device failure                   The Power On LED remains dark although the device is  switched on when there is an operation or hardware failure  In  such case  please verify that    e the power outlet supplies electricity    e the Standard Power Pack P25 is correctly connected  to the power source    e the fuses in the power inlet socket are not defective     Caution  Disconnect the device from the power    A N supply by unplugging the power cord before any  fuse replacement       14    Biometra    An Analytik Jena Company       6 2 Display Indication    The actual and set values for the voltage and current outputs  will alternatively be shown on the LCD display  see chapter  5 4 Monitoring Voltage and Current   The two LEDs on the  right side of the display indicate to which parameter the value  shown on the display belongs  The following rules apply        LED indication Display indication     mA        LED illuminated   Output current in mA     VOLT        LED illuminated   Output voltage in volt     mA        LED flashes Set value for the current  control in mA     VOLT        LED flashes Set value for the voltage  control in volts                            15    Biometra  An Analytik Jena Company       6 3 Operating Status Display    The Standard Power Pack P25 is in one of the following three  operating modes    voltage limitation        current limitation    and    
3.  de   sign  The design and manufacture of parts have been  conceived with one purpose   to produce units which will  give satisfactory service     Biometra GmbH guarantees this unit to be free from de   fects in materials or workmanship under normal use or  service for 24 month from date of shipment     If  during this time  this unit proves defective in materials  or workmanship  Biometra GmbH will repair or replace it  free of charge if returned to us prepaid     This guarantee does not cover damage in transit  dam   age caused by carelessness  misuse or neglect  or un   satisfactory performance as a result of conditions beyond  our control  or consequential losses as a result of failure  of our product              25    Biometra    An Analytik Jena Company       Biometra GmbH  Service Department  Rudolf Wissell Str  30 Rudolf Wissell Str  14 16  D 37079 Goettingen D 37079 Goettingen    Phone   49  0 5 51 50 68 6 0 Phone   49  0 5 51 50 68 6 10 or  12  Fax   49  05551 50686 66 Fax   49  0 551 50 68 6 11  e mail  Info biometra com e mail  Service biometra com  internet  http   www biometra de    26    
4.  power limitation    depending on the selected voltage and  current settings and the ohmic resistance of the  electrophoresis chamber  The operating mode is indicated by  the two LEDs located below the display  The following rules    apply                       LED indication Display indication     CURRENT        LED Current limitation  illuminated     VOLTAGE      LED illuminated   Voltage limitation     CURRENT        LED and Power limitation     VOLTAGE        LED illuminated          In the    voltage control    mode the output voltage will remain  equal to the selected value  see also chapter 5 5 Working with  Constant Voltage      Should the output current exceed the selected default value   the    current limitation    function of the Standard Power Pack  P25 is activated  The output voltage is now adjusted to the  ohmic resistance of the electrophoresis chamber  The output  current remains limited to the selected default value  see also  chapter 5 6 Working with Constant Current      Should the user   s selected voltage and current defaults  exceed the maximum allowable output power of 200W  the  device will reduce the maximum allowable output current  according to figure 1 in chapter 5 7  Both LED indicators are  illuminated when the current limitation is accessed to indicate  that the output current  due to the    power limitation     is lower  than the value originally set by the user     16    Biometra    An Analytik Jena Company    7 0 Cleaning    Disconnect the 
5.  should not exceed 300mA  The following set values  will be applied     Output voltage  150V  Output current  300mA    10    Biometra    An Analytik Jena Company       5 6 Working with Constant Current    Adjust the current controllers set value to the desired output  current  see chapter 5 3 Current Setting  when working with  constant current  The voltage controller s set value will then  have been selected so high that the device will switch to  current limit mode  see chapter 5 2 Voltage Setting      When the current output has reached the desired set value   the Standard Power Pack P25 will limit the current  The  voltage output will then adjust to the ohmic resistance of the  electrophoresis chamber and the current output will remain  constant     The LED indicator    CURRENT     see chapter 6 3 Operating  Status Display  is illuminated throughout the current limitation  mode     Should the set value for the voltage controller be  VAN selected too low  the output voltage will therefore not  suffice to reach the desired current     The output power of the Standard Power Pack P25 is   VAN limited to 200W  The maximum allowable current  output of the device is limited to 200V as shown in  figure 1 of chapter 5 7     Application example    A semi dry blot should be conducted with a constant current of  800 mA  For safety reasons the output voltage should not  exceed 50V  The following set values will be applied     Output voltage  50V  Output current  800mA    11    Biometr
6. Biometra    An Analytik Jena Company          Standard Power Pack P25    Order No  040 800    Manual               Attention    VAN Please read this manual carefully VAN  prior to operating the device             Biometra GmbH   Rudolf Wissell StraBe 30  D 37079 G  ttingen  P O  Box 1544  D 37005 G  ttingen   Phone    49      0 5 51   50 68 6 0   Fax    49      0 5 51   50 68 6 66   E mail  Info biometra com   Internet  http   www biometra com    Service Division   Rudolf Wissell Stra  e 14 16  D 37079 G  ttingen  Phone    49    0 5 51   50 68 6 10 or  12   Fax    49      0 5 51   50 68 6 11   E mail  Service biometra com          Biometra    An Analytik Jena Company       This document describes the state at the time of  publishing  It needs not necessarily agree with fu   ture versions     Subject to change        Standard Power Pack P25 Manual  2010 11    Biometra    An Analytik Jena Company             1 0 Intended Uses and Specification       The Biometra Standard Power Pack P25 is suitable for  PAGE  SDS PAGE  agarose gel electrophoresis  blotting and  electroelution of proteins and DNA RNA from gels     The Standard Power Pack P25 is intended for use with any  electrophoresis applications designed to operate below 400  volts and 500 milliamps DC  electrophoresis  or 200 volts and  1000 milliamps DC  blotting   Four sets of outputs operate in  parallel to provide 0 to 400 volts  up to 1000 milliamps  and up  to 200 watts in constant voltage or constant current mode   Vo
7. Power Pack  Use only mild  non abrasive  detergents and a slightly moistened soft cloth  Do not use  solvents  soak while cleaning or immerse in water        17    Biometra    An Analytik Jena Company          8 0 Service    Should you have any problem with the device  please contact  our service division or your local Biometra dealer     Biometra GmbH   Service Division   Rudolf Wissell StraBe 14 16  D 37079 G  ttingen  Phone    49    0 5 51   50 68 6 10 or  12   Fax    49    0 5 51   50 68 6 11   E mail  Service biometra com    Please follow the return instructions when returning the  device     Instructions for return shipment    e Return only defective devices  For technical problems which are  not definitively recognisable as device faults please contact the  Technical Service Department at Biometra   Tel    49  0 5 51 50 88 1 10 or  12  Fax   49  0 5 51 50 88 1 11   e mail  Service biometra com      e Please contact our service department for providing a re   turn authorization number  RAN   This number has to be  applied clearly visible to the outer box  Returns without  the RAN will be not be accepted     e Important  Carefully clean all parts of the instrument  from residues  and of biologically dangerous     chemical or radioactive contaminants  If an  VAN instrument is contaminated  Biometra will be    18    Biometra    An Analytik Jena Company       forced to refuse to accept the device  The sender of the re   pairorder will be held liable for possible damages and
8. a    An Analytik Jena Company       5 7 Power Limitation    The output power of the Standard Power Pack P25 is limited  to 200W  Should the user s selected voltage and current  defaults exceed the maximum allowable output power  the  device will reduce the default set value of the output current as  shown in figure 1     1A        Power   Par Limitation  5 a        0 5 A        5  O   OA   OV 200 V 400 V  Output Voltage    Figure 1  Automatic limitation of the output current at high  output voltage values    The    CURRENT    and    VOLTAGE    LED indicators are both  illuminated when the current limitation is accessed  see  chapter 6 3 Operating Status Display  to give a signal that the  output current  due to the    power limitation     is lower than  the value originally set by the user     12    Biometra    An Analytik Jena Compan          5 8 Switching Off    The Standard Power Pack P25 can be turned on or off by  pressing the on off switch     The device must always be turned off before the  supply line to the electrophoresis chamber is to be  connected or removed for any given reason  power  switch on    0    and LED above not illuminated      13    Biometra    An Analytik Jena Company    6 0 Status Notification    6 1 Power ON LED       A green LED located above the on off switch shows  along  with the on off switch position  if the Standard Power Pack  P25 works properly              on off switch LED Meaning  position indication     O    dark No error   Power off   
9. bly to the operating instructions will dissolve any  responsibility and liability        Observe the relevant rules for accident prevention  as well as those of approved safety and  occupational medicine     Do not attempt any repair yourself  In need of  repair  consult your local qualified service  representative  Only qualified personnel may open  the housing parts  The inner device may still  contain dangerous voltage several minutes after  disconnection from power supply     Make sure the indicated voltage and frequency  range of the device corresponds with the system  voltage and that the power switch is on    0    before  it is connected to the power supply     Connect the device to a properly grounded outlet  only     Pull the power cord out of the outlet to safely  disconnect from the power supply      gt  gt   gt  PP     gt   gt      gt      gt   gt     Biometra    An Analytik Jena Compan          Use exclusively the provided power cord or one  that meets the legal requirements     Pay attention to the dimensioning of the fuses and  replace them if required  The fuse holder is located  in the lower part of the power connector on the  back side of the Power Pack  The following fuses  are required     Micro fuses  slow blow 2 x 5A  250 V     Caution  For continued protection against risk of  fire  replace only with same type and rating of  fuse       Caution  Double pole neutral fusing  disconnect  power before changing fuse     In order to ensure the safe electrical 
10. disconnection  the floating outputs must never be grounded   Make sure that your test setup always complies to  this condition     Only safe electrical cords certified according to  IEC   EN 61010 2 031 may be connected to the  power outputs     Make sure the Standard Power Pack P25 is  located in a safe and dry place  in proximity to the  electrophoresis devices powered  Keep away from  liquids  Should a liquid penetrate the P25   disconnect the device immediately      gt  gt  e b amp b D    Biometra    An Analytik Jena Compan          Do not operate in a damp or humid environment  where condensing moisture may lead to creeping  current  See chapter 2  Technical Data  for  ambient operating temperature range     When moving from a cold to a warm room  allow 8  hours before reusing the device in order for it to  reach the ambient temperature and for possible  condensation to evaporate     Use only mild  non abrasive detergents  Carefully  clean the device to avoid liquids penetrating the  inner housing     When positioning the P25  make sure the  ventilation openings on the back side are not  covered     Should the Power Pack become extremely warm   smoke or display unusual information while in  operation  discontinue use immediately and  contact a qualified service representative     Biometra    An Analytik Jena Company    4 0 Set Up    4 1 Content       e Standard Power Pack P25  e Manual  e Power Cord    4 2 Unpack and Check    Your Biometra Standard Power Pack P25 has bee
11. electrophoresis chamber s   before switching on the Standard Power Pack P25  Then  switch on the device using the power switch  A green light  appears over the power switch when power is turned on        When power is turned on  the P25 sets the output  voltage or current to the latest value stored     Should you be unsure of the latest saved settings  detach all  connections to the electrophoresis chamber and switch on the  Standard Power Pack P25 in order to check the voltage or  current settings  see chapter 5 2 Voltage Setting and chapter  5 3 Current Setting      The device must always be turned off before the  supply line to the electrophoresis chamber is to be  connected or removed for any given reason  power  switch on    0    and LED not illuminated      5 2 Voltage Setting    By using the    SELECT    button  switch to    VOLT    on the  display  Setthe desired output voltage by turning the knob     In normal mode  the display shows the output voltage  actual  value   By turning the knob  the display changes from the  actual value to the desired set value  recognizable by the  flashing    VOLT    LED indicator  The desired value increases  with every clockwise rotation and decreases with every  counter clockwise rotation  Depending on speed and direction  of the rotation  the value will augment or reduce in steps of 1V     7    Biometra    An Analytik Jena Compan          10V or 100V  A set value modified by the user will  3 seconds  after the last operation of the 
12. knob  precede all other values  and will be set as the desired output voltage     The voltage value  V  should be set without any  device connected     5 3 Current Setting    By using the    SELECT    button  switch to    mA    on the display   Set the desired output current by turning the knob     In normal mode  the display shows the current conduction   actual value   By turning the knob  the display changes from  the actual value to the desired set value  recognizable by the  blinking    mA    LED indicator  The desired value increases with  every clockwise rotation and decreases with every counter  clockwise rotation  Depending on speed and direction of the  rotation  the value will augment or reduce in steps of 1mA   10mA or 100mA  A set value modified by the user will  3  seconds after the last operation of the knob  precede all other  values and will be set as the desired output current     The electric current value  mA  should be set  without any device connected     Biometra    An Analytik Jena Company       5 4 Monitoring Voltage and Current    5 4 1 Monitoring Set Values    Choose the desired measurement category by using the     SELECT    button in order to monitor the default voltage and  current values  Then turn the knob to the right or to the left by  one detent and the LCD displays will show the desired set  value of the corresponding measurement  The value  appearing on the display will  3 seconds after the last  operation of the knob  precede all other va
13. ltage and current setting is adjustable in steps of 1V and  1mA     A single LCD display provides output voltage or current  readings        Biometra       An Analytik Jena Company       2 0 Technical Data     2 0 Technical Data OL      Type Output   Max  Voltage   Max  Current   Max  Power     Output Display     Accuracy     Number of Output  Terminals     Input Power   Allowable deviation     Ambient Operating  Temperature Range     Humidity   Dimensions   Weight     Const  Voltage and Const  Current  400 VDC   1000mA in the range 0 200VDC   lt 1000mA in the range 200 400VDC  200 W  continuous    LCD display  3 5 digits  selectable  between display mA  and V  Resolution 1 mA and 1 V  respectively       5  v M    for U     gt   0 05   Umax  Imax      5  of 0 05   Umax  Imax     for U I  lt  0 05   Umax  Imax   Arithmetic mean over 3 s  Ts   0 4 s    Four sets of 4 mm safety sockets    100   240VAC 50 60Hz max  300W   6  10     0 to 40  C   0 to 70     W x Lx H  26 5 x 25 0 x 9 5 cm  3 kg    The Standard Power Pack P25 is designed for use with AC  net supply voltages between 100 Volt and 240 Volt  50 60Hz   It is a switched mode power supply providing voltage and  current controlled DC power  respectively  The DC output is  free of ground and short circuit proof          See also chapter 5 7    Biometra    An Analytik Jena Company    3 0 Safety Instructions    The Standard Power Pack P25 must only be used in  accordance with its designation  The use of this device  unconforma
14. lues  The LCD  display will then automatically be switched to the actual value     5 4 2 Monitoring Actual Values    Choose the desired measurement category at any time by  using the    SELECT    button in order to control the output  voltage and current  In doing so  only the LCD display will be  switched  The supplied current or applied voltage will not be  affected by this operation     5 5 Working with Constant Voltage    Adjust the voltage controllers set value to the desired output  voltage  see chapter 5 2 Voltage Setting  when working with  constant voltage  Then select the maximum possible or  allowable value for the current controllers set value  The  current controllers set value will then be the maximum  possible or allowable value chosen  see chapter 5 3 Current  Setting      The Standard Power Pack P25 supplies a constant output  voltage until reaching the selected current limit  voltage  control   The output current then varies according to the ohmic  resistance of the electrophoresis chambers  The output of a    9    Biometra    An Analytik Jena Company       constant voltage will be shown by the LED indicator     VOLTAGE      see chapter 6 3 Operating Status Display      Should the maximum allowable output current be exceeded   the Standard Power Pack P25 will limit the current and the  output voltage will drop     Application example    An agarose gel electrophoresis should be conducted with a  constant voltage of 150V  For safety reasons the output  current
15. n carefully  packed to ensure its safety  Please unpack it carefully  control  the integrity of its content and inspect the device  Report any  damage immediately to BIOMETRA     Do not operate a device which is in no proper condition   Should you have a complaint  return the complete system in    its original packing by following the return instructions  see  chapter 8 0          N Attention  AN    Please send back the enclosed postcard along with  the filled out Warranty Certificate to Biometra in  order to claim the full warranty           Biometra    An Analytik Jena Compan          4 3 Power Supply    Before connecting  make sure the actual voltage complies with  the voltage range indicated on the type label     The Standard Power Pack P25 must be    grounded     4 4 Location    The Standard Power Pack P25 must be located in a dry and  safe place  Do not place an electrophoresis chamber on the  power supply device  The ventilation openings on the back  side must not be covered by other devices  furniture or  fixtures  A sufficient air flow for convection cooling must be  provided     4 5 Electrophoresis Chamber Connection    The electrophoresis chamber is connected to the Standard  Power Pack P25 by safety cords  Use exclusively  electrophoresis devices with safety connectors     Switch off the Standard Power Pack P25 while  working on the test equipment setup     Biometra    An Analytik Jena Company       5 0 Operation    5 1 Power On    Establish the connection to the 
16. od of cleaning   decontamination              The equipment has been cleaned and decontaminated     NAME POSITION   HEAD OF DIV   DEP   INSTITUTE   COMPANY           SIGNED DATE          PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL  BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT   PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING   INSTRUMENTS WITHOUT THIS CERTIFICATE ATTACHED WILL BE  RETURNED TO SENDER     20       Biometra    An Analytik Jena Company       General Information for Decontamination     Please contact your responsible health  amp  safety officer for  details     Use of radioactive substances   Please contact your responsible person for details     Use of genetically change organism or parts of those   Please contact your responsible person for details     21    Biometra    An Analytik Jena Company             10 0 Note for Disposal of  Eletric Electronic Waste                This symbol  the crossed out wheelie bin  means  that  this product should be brought to the return systems  and or separate systems available to end users according  to yours country regulations  when this product has  reached the end of its lifetime     For details  please contact your local distributor   This symbol applies only to the countries within the EEA       EEA   European Economics Area  comprising all EU   members plus Norway  Iceland and Liechtenstein     22    Biometra    An Analytik Jena Company    11 0 Notes          23    Biometra    An Analytik Jena Compan
17. y             12 0 EU   Konformit  tserkl  rung  EC   Declaration of Conformity       G  ttingen  2010    im Sinne der EG Richtlinie   ber elektrische Betriebsmittel zur Verwendung  innerhalb bestimmter Spannungsgrenzen 73 23 EWG   following the EC directive about electrical equipment for use within certain  limits of voltage 73 23 EEC    und   and    im Sinne der EG Richtlinie f  r die elektromagnetische Vertr  glichkeit  89 336 EWG    following the EC directive about the electromagnetic compatibility  89 336 EEC      Hiermit erkl  ren wir  da   das folgende Power Pack   Herewith we declare that the following Power Pack     Typ   type  Standard Power Pack P25  Rev  b   Best  Nr    Order No  040 800    den grundlegenden Anforderungen der  corresponds to the basic requirements of    EG Niederspannungsrichtlinie 73 23 EWG und der  EC low voltage directive 73 23 EEC and the    EG Richtlinie   ber die elektromagnetische Vertr  glichkeit 89 336 EWG  entsprechen   EC directive about the electromagnetic compatibility 89 336 EEC      Folgende harmonisierte Normen wurden angewandt   The following harmonized standards have been used     EN 60601 1 2 2001   EN 55011 1998   A1 1999   A2 2002 EN 61000 3 2 2000  EN 61000 3 3 1995   A1 2001 EN 61000 6 1 2001  EN 61010 1 2001    Dr  J  rgen Otte  Quality Manager    24       Biometra    An Analytik Jena Company    13 0 Warranty          This laboratory instrument is produced with the highest  practical standards of materials  workmanship  and
    
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