マニュアル Monitor UVis-920
Contents
1. Flow Cell 10mm SN123456 Check Analog Out Check Analog Out Testing Reference information 6 6 5 Checkmenu 6 5 1 Autozero checking autozero level The instruments internal absorbance value for autozero can be checked to test the consistency in buffers 1 Select menu Check press OK 2 Select menu Autozero The autozero absorbance value is shown 6 5 2 Flow cell checking flow cell type and number This menu shows the type and serial number of the flow cell stored in the monitor 1 Select menu Check press OK 2 Select menu Flow Cell If serial number is not set is shown The serial number must be 1000 or higher 6 5 33 Check analog output The function of the connected chart recorder can be tested 1 Select menu Check press OK 2 Select menu Check Analog Out press OK 3 Start the test press OK The test will ramp the channel up to 1 V and then decrease the signal 1V in 10 steps back to O V The test is run continuously Compare the diagram of the chart recorder with the figure ov 4 Stop the test by pressing Esc Monitor UVis 920 11 0031 12 Edition AC 43 6 Reference information Light Intensity R 1150mV S 1800mV Filter Used Time 024h Lamp Used Time 200h Software Version V1 00 00 44 6 5 4 Light intensity This menu shows the voltages generated at the light detectors These voltages are proportional to th2. e Industrial flow cell 8 mm tubing i d 8 mm e Industrial flow cell 1 tubing i d 1 2 4 2 Installing UV Flow Cell 2 mm and UV Flow Cell 10 mm Unpack the flow cell Do not remove the red protective caps from the inlet or outlet or the black protective caps on the optical fiber connectors 1 Slide the rear white clip on the cell holder to its inner position for a 2 mm cell and to its outer position fora 10 mm cell Fig 2 3 Cell holder with clips for holding the cell 2 Place the flow cell in the opening between the white clips The cell should be positioned with the text and serial number facing upwards or sideways Press the cell into the clips to fasten it CAUTION To avoid damaging the optical fibers press only on the cell body never on the optical fibers Monitor UVis 920 11 0031 12 Edition AC 11 2 12 Installation To improve the access to the tubing connections the flow cell can alternatively be positioned in the cell holder Normal position Alternative position Serial number Fig 2 4 The positions of the flow cell Connecting the optical fibers 1 Removethe two black protective caps from the optical fiber connectors CAUTION Do not touch the tips of the optical fibers with your fingers as this will result in poor monitor performance If you accidentally touch the optical fiber tip it can be wiped with 30 isopropanol using lens papers WARNING The in
3. Reference information 6 Industrial flow cell 8 mm Recommended max flow rate Max pressure 2 MPa 20 bar 290 ps up to 40 C Backpressure Max 0 1 bar at 1800 I h with water at 25 C Liguid temperature range 4 to 80 C at max 0 1 MPa Optical path length 1 2 5 mm Cell volume 2 5 ml Wetted materials PEEK polyetheretherketone Titanium palladium alloy Quartz synthetic fused silica PFR 91 or EPDM pH stability range 1 13 13 14 lt 1 days exposure Chemical resistance The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules except 100 Ethyl acetate 100 Hexane and 100 Tetrahydrofuran THF or 15 THF in acetonitrile Tubing connections TC25 connector See pressure flow graphs in UV 900 industrial flow cells instructions 28 4051 40 49 Monitor UVis 920 11 0031 12 Edition AC 6 Reference information 50 Industrial flow cell 1 Recommended max flow rate Max pressure 1 MPa 10 bar 145 psi up to 40 C Backpressure Max 0 4 bar at 600 I h with water at 25 C Liquid temperature range 4 to 80 C at max 0 1 MPa Optical path length 1 2 5 mm Cell volume 12 ml Wetted materials PEEK polyetheretherketone Titanium palladium alloy PFR 91 or EPDM pH stability range 1 13 1 14 lt 1 days exposure Chemical resistance The wetted parts are resistant to organic solvent
4. Transmitter KS NOA Knurled nut Protective cap Fig 2 8 Connection of optical fibers from industrial flow cell to UVis 920 Monitor UVis 920 11 0031 12 Edition AC Installation 2 Note The optical fiber with the gold colored knurled nut is the transmitter fiber and must not be mixed up with the other fiber the receiver fiber 10 Connect the other optical fiber to the empty socket on the flow cell and to the rear fiber connector on the monitor CAUTION To avoid damaging the optical fibers press only on the cell body never on the optical fibers Monitor UVis 920 11 0031 12 Edition AC 15 2 Installation 2 5 Connecting electrical cables The sockets for electrical signals are located on the rear panel Analog out 0 1 V for recorder Service connectors On off switch Remote connector Mains connector for auxiliary equipment Fig 2 9 UVis 920 rear panel 2 5 1 Connecting to recorder Connect the recorder to the miniDIN socket Analog out 0 1 V using the cable supplied The absorption signal is available on a channel using the following wires Wire 1 signal Wire 2 ground Note The signal cable is delivered with protective covers on each wire Do not remove the protective covers from unused connections as a short circuit may disturb the measurements Set the recorder to 0 1 V input full scale O V offset 2 5 2 Conne
5. 1 3 8 33 Other menus The other setting menus are described in the reference section see 6 4 Setup menu 27 3 Operation 28 3 9 Storage Overnight The flow cell can be left filled with buffer Weekend and long term storage Flush the flow cell with distilled water and then fill it with 20 ethanol The flow cell can also be stored dry by flushing as above with distilled water and then blowing a compressed inert gas such as nitrogen N3 through the cell Replace the protective caps Never use compressed air as this may contain droplets of oil CAUTION Do not allow solutions which contain dissolved salts proteins or other solid solutions to dry out in the flow cell Do not allow particles to enter the flow cell as damage to the flow cell may occur 3 10 UV cell calibration For exact measurements of the nominal flow cell path length the path length in the UV flow cell can be calibrated This is not necessary for standard operations Call GE Healthcare for advice 3 11 Changing flow cell The flow cell can be changed when reguired for example from 2 mm to 10 mm when the sensitivity of the measurement must change due to a small amount of sample being applied or from a 10 mm to 2 mm when a lower sensitivity is desired due to output signal limitation See section 2 4 Installing the flow cell Data for up to five flow cells are saved in memory in UVis 920 3 12 Restart after power failure If the power supply
6. 3 If yes enter the wavelength of custom filter in nm Press OK Monitor UVis 920 11 0031 12 Edition AC 41 6 Reference information 6 4 5 Resetting filter time The time the current filter has been used can be shown in hours and resetted to zero There is one time counter for each filter wavelength Reset Filter Time 1 Select menu Reset Filter Time press OK 024h 2 Display toggles between yes and no Select yes to reset the time counter for the current filter wavelength press OK Reset Filter Time yes no 6 4 6 Setting run and end timers The UV monitor can be started or stopped automatically using countdown timers RunTimer End Timer 1 Select main menu RunTimer End Timer press OK 057min 123min Set Run Timer 2 Setrun time press OK to select action Choose the Run mode time 1 999 057min minutes Timer is disabled if set to O If timer is enabled by OK the remaining Set Run Timer minutes to run are shown min 68 Set End Timer 3 Set end time press OK to select action Choose the End mode time 1 999 123min minutes Timer is disabled if set to O If timer is enabled by OK the remaining Set End Timer minutes to end are shown min 150 4 Press Esc key to return to the RunTimer End Timer menu which now shows the countdown time and end time 6 4 7 Setting unit number For service use only 42 Monitor UVis 920 11 0031 12 Edition AC Autozero 215nm 0 23454AU
7. Degree of protection IP 20 Monitor UVis 920 11 0031 12 Edition AC Reference information 6 Compliance with standards The declaration of conformity is valid for the instrument only if it is e used in laboratory locations e used in the same state as it was delivered from GE Healthcare except for alterations described in the User Manual e used as stand alone unit or connected to other CE labelled GE Healthcare instruments or other products as recommended Safety Standards This product meets the requirement of the Low Voltage Directive LVD 73 23 EEC through the following harmonized standards e EN 61010 1 2001 e EC 61010 1 e CAN CSA C22 2 No 61010 1 e UL61010 1 EMC Standards This device meets the requirements of the EMC Directive 89 336 EEC through the following harmonized standards e EN 61326 emission and immunity e EN 55011 GR 2 Class A emission e This device complies with part 15 of the FCC rules emission Operation is subject to the following two conditions 1 This device may not cause harmful interference 2 This device must accept any interference received including interference that may cause undesired operation Monitor UVis 920 11 0031 12 Edition AC 47 6 Reference information 48 UV Flow Cell 2 and 10 mm Recommended max flow rate 100 ml min Max pressure 2 MPa 20 bar 290 psi Backpressure Max 0 5 bar at 2 ml min wi
8. needs to be removed 1 Insert a filter unit in the monitor The instrument reads the code tag of the inserted filter unit and display its wavelength in the main menu If the filter is ejected the display shows A nm Code tag Filter Filter holder Filter unit Filter uni Fig 3 8 Inserting a filter unit Empty filter holders for custom filters are available When using a custom filter its wavelength can be stored in the instrument by the operator See Chapter 6 4 4 Setting wavelength for custom filter CAUTION Filter units must be stored in a clean and dustfree place CAUTION Do not run the monitor with an empty filter holder Monitor UVis 920 11 0031 12 Edition AC Operation 3 3 5 Setting lamp on off The Xenon lamp is switched on when the run end key is pressed No warm up time is reguired The lamp should be switched off when no measurement is made standby to conserve the lamp operating time 1 Select the main operating menu 2 Switch the lamp on running or off standby by pressing run end The monitors current status is shown in the lower right of the display To setting a run timer or end timer is another way to switch the Xenon lamp on off See section 6 4 6 Setting run and end timers 3 6 Reading absorbance values The main operating menu shows the absorbance values The menu is reached from any other menu by pressing the Esc key repeatedly 3 7 Autozero The a
9. 39 Main operating menu MAIN WINDOW ccccccsssssseseccssssseessssssseeees 40 Setup MENU EE Check menu Custom filters N Technical spociTICatlons yssen naika 45 Accessories and spare parts smmumuuuuuuesmmmmuumm states tata 50 Monitor UVis 920 11 0031 12 Edition AC Introduction 1 1 Introduction 1 1 General Monitor UVis 920 is a UV and visible light absorption monitor for use in liguid chromatography Monitor UVis 920 features e Stand alone use e Accurate and reliable monitoring e Detection in the wavelength range 200 700 nm e Possibility to select wavelength in the above range by changing filter unit only e Flow cells in range from laboratory use to industrial use are available e No toxic mercury lamp e Nolamp warm up time allowing lamp on off function Fig 1 1 Monitor UVis 920 with flow cell holder Monitor UVis 920 11 0031 12 Edition AC 7 1 Introduction 1 2 Safety IMPORTANT Monitor UVis 920 plus is intended for laboratory use only not for clinical or in vitro use or for diagnostic purposes e The instrument is designed for indoor use only e Donotuse the instrument in a dusty atmosphere or close to spraying water WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation and maintenance of the syste
10. GE Healthcare Monitor UVis 920 User Manual Important user information All users must read this entire manual to fully understand the safe use of Monitor UVis 920 WARNING The WARNING sign highlights instructions that must be followed N to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION The CAUTION sign highlights instructions that must be followed to avoid damage to the product or other eguipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the reguirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on reguest The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used asa laboratory device It is not intended for clinical or in vitro use of for diagnostic purposes used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disp
11. UV monitor o filter in holder Check that there is a filter in the filter holder Filter clogged Check the filter for obvious obstacles restraining the light nternal error Call GE Healthcare Low transmission The transmission in the flow cell is too low due to air bubbles or too high absorption in the buffer Flush cell and or change buffer Low light intensity See section 6 5 4 Light intensity Serial no not valid Serial number entered from display is not valid Enter a correct serial number Must be 1000 or higher Error 100 The UV signal is not stable during calibration See 5 2 for corrective actions Error 250 Undefined error Switch off the instrument Switch on the instrument If the error display remains call GE Healthcare Table 5 2 Error messages Monitor UVis 920 11 0031 12 Edition AC Troubleshooting 5 Monitor UVis 920 11 0031 12 Edition AC 35 6 Reference information 6 Reference information 6 1 Description 6 1 1 Instrument The Monitor UVis 920 is a UV and visible light absorption monitor which can be used in wavelengths range between 200 700 nm by changeable fixed wavelength filters The instrument contains no internal user replaceable items Analog out o PO Voltage 100 240 Frequency 50 60 H Power max IVA Fig 6 1 Monitor UVis 920 and its rear panel C
12. ain window shows the wavelength in nanometers nm the cell path length CL in millimeters mm the absorbance value in AU and the status mode The main window is reached from any other menu by pressing Esc repeatedly 6 4 Setup menu 6 4 1 Setting analog out See section 3 8 1 Setting analog output to an external chart recorder 6 4 2 Setting averaging time See section 3 8 2 Setting averaging time filtering noise 6 4 3 Setting cell serial number cell type and cell path length It is possible to store cell data in the instrument for up to five different cells The data is uniguely defined by each cells serial number 1 Select menu Set Cell Serial Number press OK 2 Enter the serial number of the cell see the label on the flow cell Press OK The serial number must be 1000 or higher 3 Select menu Set Cell Type Check the cell type value If the value is correct use the arrow keys to go to next menu If the value has to be changed press OK to change it 4 Select menu Set Cell Path Length press OK 5 Enter the calibrated value of the cell path length if available press OK The cell type must always be set before the calibrated cell path length 6 4 4 Setting wavelength for custom filter If a custom filter is inserted the wavelength can be set 1 Insert a custom filter The display shows Change Wave Length 2 Select yes to set wavelength press OK Select no press Esc to return to main display
13. ble if a custom filter is inserted Reset Filter Time RunTimer EndTimer Unit Number 0 Check Analog Out Filter Used Time i Lamp Used Time its V1 00 00 overview Set Range Set Zero Level 10 Set Averaging Time 2 56s lt 2 56 gt Set Cell Serial Number 123456 lt 123456 gt Set Cell Type 2mm lt 2 00 gt Set Cell Path Length 2 00mm 2 00 Set Wave Length 351nm Reset Filter Time yes no Set Run Timer Set End Timer 123 yes nof Set Range 0 050AUFS lt 0 050 gt Set Zero Level 10 10 Set Cell Serial Number 123456 123456 The serial number must be 1000 or higher Set Wave Length 351nm 387 Set Run Timer min 027 Set End Timer min 058 U Check Analog Out OK Testing Esc Press Esc to leave this display 2 lt 2 gt Selecting main menu sub menus and setting of values Menu or selection accepted Moves back one menu level or cancel a value Open values within range Preset values New value to be set underscore Monitor UVis 920 11 0031 12 Edition AC A 215nm CL 10 00mm 0 00000AU STANDBY Set Cell Serial Number 123456 000000 Set Cell Type 2mm lt 2 gt Set Cell Path Length mm 02 10 Change Wave Length 350nm yes no Set Wave Length 350nm 351 Reference information 6 6 3 Main operating menu MAIN WINDOW The main operating menu m
14. cting to auxiliary equipment if used Connect any auxiliary equipment to the 9 pole D Sub female Remote connector 5 V TTL signals only See section 6 1 1 Instrument for more information 16 Monitor UVis 920 11 0031 12 Edition AC Installation 2 2 5 3 Connecting to supply voltage WARNING Only use mains cables delivered or approved by GE Healthcare 1 Make sure the on off switch is in the OFF position O 2 Connect a mains cable between the instrument and a grounded mains socket The instrument is delivered with both European and US type mains cables as standard Any voltage 100 240 V 50 60 Hz can be used WARNING The instrument must be connected to a grounded mains socket The instrument contains no user replaceable fuse WARNING Do not block the rear panel of the instrument The mains power switch must always be easy to access gt gt Monitor UVis 920 11 0031 12 Edition AC 17 2 Installation 18 Monitor UVis 920 11 0031 12 Edition AC Operation 3 3 Operation 3 1 Menu selection and settings WARNING When using hazardous chemicals take all suitable protective N measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation and maintenance of the instrument 3 1 1 UVis 920 front panel Operation menu and settings are selected by the membrane keys at the front of th
15. e UVis 920 OUVis 920 Display 2 x 20 characters Membrane keys Filter unit insert Eject button for filter unit Fig 3 1 UVis 920 front panel run end key Selection of running or end standby mode event mark key Event mark can be set and displayed as a spike on the chart recorder for example when a sample is injected autozero key Sets the detected absorbance to zero for example after baseline is stable and before the sample is injected Monitor UVis 920 11 0031 12 Edition AC 19 3 Operation 3 1 2 Menu selection A specific menu is selected by the front arrow down key When the reguired menu is visible the menu or selection is accepted by pressing the OK key Fig 3 2 Menu selection 20 Monitor UVis 920 11 0031 12 Edition AC Monitor UVis 920 11 0031 12 Edition AC Operation 3 If a menu has sub levels the sub menu is displayed by pressing the OK key Pressing the Esc key moves back one menu level Main menu Sub menus Fig 3 3 Menu navigation Return to main menu Pressing Esc repeatedly always returns to the main menu and the main operating window Main operating A m n O ESC ESC ESC ESC Fig 3 4 Return to main window by pressing Esc repeatedly 21 3 Operation 22 Select value A cursor below a text or numerical value shows what is affected by a key To increase the value press arrow up key To decrease the value pres
16. e light intensities in each channel The R value gives an indication of the condition of the lamp filter and optical fibers 1 Select menu Check press OK 2 Select menu Light Intensity If the R value is lt 750 mV check filter used time Change filter if the used time is gt 2500 hours If the R value still is lt 750 mV after replacing filter contact GE Healthcare 6 5 5 Filter used time This menu shows how many hours the filter has been used i e the time in running mode There is one time counter for each filter wavelength 1 Select menu Check press OK 2 Select menu Filter Used Time 6 5 6 Lamp used time 1 Select menu Check press OK 2 Select menu Lamp Used Time If the lamp used time is gt 4000 hours contact GE Healthcare for lamp replacement 6 5 7 Software version 1 Select menu Check press OK 2 Select menu Software Version Monitor UVis 920 11 0031 12 Edition AC Monitor UVis 920 11 0031 12 Edition AC Reference information 6 6 6 Custom filters If a wavelength not covered by available filter units is needed the empty filter holder together with a custom filter can be used The filter should have a diameter of 12 5 mm and a maximum thickness of 6 5 mm to fit in the holder The transmission of the filter must correspond with the working range of the monitor This can be checked from the light intensity menu where the R value must be between 2000 and 4500 mV when using the filter If the va
17. ent temperatures in the range 4 to 40 C Normal room temperature is recommended The instrument is designed as a stand alone unit and should be placed on a stable laboratory bench To ensure correct ventilation a free space of 0 1 meter is required behind and in front of the instrument Place the instrument directly on the bench The mains power switch on the rear panel must always be easy to access Monitor UVis 920 11 0031 12 Edition AC 9 2 Installation 2 3 Installing the cell holder If UV Flow Cell 2 mm or UV Flow Cell 10 mm is to be used the flow cell holder included in the UVis 920 delivery must be installed 1 Unpack the cell holder 2 Slide the cell holder in place on the right hand side of the UVis 920 Fig 2 1 Installing cell holder for UV Flow Cell 3 Fasten the flow cell holder with the two screws included Use a hex wrench Fig 2 2 Fastening the flow cell holder CAUTION To avoid damaging the optical inlet and outlet the two black rubber caps at the optical fiber connectors must be in place when installing the cell holder disconnect the optical fibers while the monitor is running WARNING The instrument uses high intensity ultra violet light Do not 10 Monitor UVis 920 11 0031 12 Edition AC Installation 2 2 4 Installing the flow cell 2 4 1 General The following flow cells are available e UVFlowCell2 mm e UV Flow Cell 10 mm e UV cell 1 2 5 mm AKTApilot
18. f cell holder cover Monitor UVis 920 11 0031 12 Edition AC 13 2 14 Installation 2 4 3 Installing UV cell 1 2 5 mm KTApilot Industrial flow cell 8 mm and 1 Installing the flow cell 1 Unpack the flow cell Do not remove the black protective caps on the optical fiber transmitter and receiver connector 2 Choose the optical path length of the flow cell The optical path length can be changed with shim plates 3 Attach the flow cell to an appropriate flow cell holder 4 Connect the inlet and outlet tubing to the flow cell Connecting the optical fibers CAUTION Do not touch the tips of the optical fibers with your fingers as this will result in poor monitor performance If you accidentally touch the fiber tip it can be wiped with 30 isopropanol using lens paper WARNING The instrument uses high intensity ultra violet light Do not disconnect the optical fibers while the monitor is running 5 Remove the two protective rubber caps from the optical fiber connectors of Monitor UVis 920 6 Remove the four black protective caps from the optical fiber connectors 7 Identify the fiber connector which has a gold colored knurled nut 8 Attach this connector to the flow cell by carefully inserting it into the socket and tightening the nut fingertight fits only one of the two sockets 9 Attach the other connector to the transmitter connector of UVis 920 Do not overtighten Receiver
19. ference information 52 Tubing and connectors Item Ouantity Code no O ring kit IC PFR 91 for flow cell 8 mm 1 18 1134 91 O ring kit IC EPDM for flow cell 8 mm 1 18 1134 88 Teflon tubing i d 1 8 od 3 16 3m 18 1112 47 Tubing connector for 3 16 o d tubing 10 18 1112 49 Ferrule for 3 16 tubing 10 18 1112 48 Stop plug 5 16 5 18 1112 50 Stop plug 1 16 5 18 1112 52 Union Luer female 1 16 male 2 18 1112 51 Union 1 16 female M6 male 6 18 1112 57 Union M6 female 1 16 male 8 18 1112 58 Union 1 16 male 1 16 male 2 18 1120 92 i d 0 25 mm Union 1 16 male 1 16 male 2 18 1120 93 i d 0 50 mm PEEK tubing i d 0 15 mm od 1 16 2m 18 1156 59 PEEK tubing i d 0 25 mm od 1 16 2m 18 1120 95 PEEK tubing i d 0 50 mm o d 1 16 2m 18 1113 68 PEEK tubing i d 0 75 mm od 1 16 2m 18 1112 53 PEEK tubing i d 1 0 mm o d 1 16 2m 18 1115 83 Tefzel tubing i d 0 25 mm o d 1 16 2m 18 1121 36 Teflon tubing i d 0 75 mm od 1 16 2m 18 1119 74 Fingertight connector 1 16 10 18 1112 55 Tools Item Quantity Code no Fiber detachment tool 1 18 1111 16 Monitor UVis 920 11 0031 12 Edition AC H GE imagination at work and GE monogram are trademarks of www gelifesciences com General Electric Company N KTApilot and Drop Design are trademarks of GE Healthcare companies GE Healthcare Bio Sciences AB O 2005 2007 General Electric Company Al rights reserved Bj rkgatan 30 First published Ap
20. lue is too high an aperture can be used Custom filters are not provided by GE Healthcare CAUTION Do not run the monitor with an empty filter holder 45 6 Reference information 46 6 7 Technical specifications Operating data Wavelength range 200 700 nm Changeable wavelength filter units Filters center wavelength accuracy 2 nm bandwidth lt 10nm Linearity lt 5 deviation up to 2 AU at 280 nm with Ferric Sulphate in 0 1 M Sulphuric acid Noise 1 2 short term 0 5 1 min long term 1 10 min lt 2x10 AU at 280 nm lt 2x10 AU at 280 nm Drift2 lt 2x10 AU h at 280 nm Environment 4 to 40 C 20 95 relative humidity non condensing 84 106 kPa 840 1060 mbar atmospheric pressure 1 Measured with water at 1 ml min time constant 1 second 10 mm flow cell 2 Typical values at room temperature after 2 hours with lamp on Physical data Light source Xenon flash lamp Lamp lifetime gt 4000 hours Control Stand alone or via Remote connector Power consumption 35 VA Power requirement 100 240 V 50 60 Hz Analog output 0 1 V full scale overrange function Digital inputs 5 V 1 mA current sinking lamp on off autozero event mark Display 2 rows with 20 characters each Dimensions H x W x D 200 x 160 x 262 mm without cell holder 200 x 300 x 262 mm with cell holder Weight 5 1 kg
21. m WARNING The instrument must not be opened by the user It contains high voltage circuits which can give a lethal electric shock WARNING The instrument must be connected to a grounded mains socket WARNING Always disconnect the power supply before attempting to replace any item on the instrument WARNING The instrument uses high intensity ultra violet light Do not disconnect the optical fibers while the monitor is running gt gt gt gt gt CAUTION The flow cells must not be used at pressures above specified pressure At higher pressures the flow cell may break 8 Monitor UVis 920 11 0031 12 Edition AC Installation 2 2 Installation 2 1 Unpacking Unpack the instrument and check the items against the supplied packing list Inspect the items for obvious damage which may have occurred during transportation It is recommended that all packing materials should be retained if onward transport of the instrument is expected CAUTION The following information should be read carefully to ensure that the instrument is installed correctly 2 2 General precautions The instrument should not be used in a corrosive atmosphere or in an atmosphere where deposits are liable to form on the optical surfaces The instrument should be located in a place of low temperature variations away from heat sources draughts and direct sunlight The instrument may be operated at ambi
22. nce filter mono hromator Signal COED CUE Optical fibres N Micro Controller Reference Beam RV nun splitter DOIT ITT Flow cell a Collimating Filter Unit KN optics 1 GPTTTTTTTTTTULUTTTILL N Flash lamp 100 Hz Fig 6 2 Monitor UVis 920 principle Monitor UVis 920 11 0031 12 Edition AC Reference information 6 6 1 3 Flow cell principle The optical path lengths of the flow cells are 1 mm 2 mm 5 mm and 10 mm depending on the type of cell installed The smaller flow cell is made of guartz with titanium housing and the industrial cells are made of PEEK Polyetheretherketone C L Cell Path Length in millimeter Fig 6 3 UV flow cell for laboratory use Principle The unique design of the flow cell above prevents the formation of distinct interfaces between eluent components with different refractive indices and eliminates the negative influence these would cause The precision of monitoring is enhanced by the construction of the flow cell which ensures total reflectance of light This maintains a high intensity of light to the detector The long path length combined with a small cell volume increases sensitivity Monitor UVis 920 11 0031 12 Edition AC 39 6 Reference information 6 2 Menu A 215nm 0 00000AU CL 10 00mm STANDBY Cell Serial MAIN WINDOW ine Number Set i Set Cell Path Length 2 00mm Set wavelength Only visi
23. non flash lamp gives a high intensity continuous spectrum of light The lamp is on only during the chromatographic run ensuring that its long lifetime is used in the most efficient way The light enters an interference filter based monochromator which includes a collimating system and a filter unit with a fixed specific wavelength It is possible to select wavelength between 200 700 nm by changing filter unit There are filter units available for different wavelengths There are also empty filter holders for custom filters Monochromatic light from the filter is directed to an optical fiber The light from the monochromator to the flow cell and from the cell to the detector electronics is guided by optical fibers which focus its full intensity on the liquid flow path maximizing the sensitivity of the monitoring Before entering the flow cell the monochromatic light is split in a beam splitter with 50 of the light passing through the sample fiber S and the flow cell and 50 being directed through the reference fiber R Two photodiodes with identical characteristics monitor the intensities of the measuring and reference beams The long path length and small volume of UV Flow cell 10 mm ensure very high sensitivities and high signal to noise ratios The detection system is also very stable and because the flow cell is located away from the lamp and electronics noise and drift caused by temperature variations is avoided Interfere
24. onnector switch Function Analog out 0 1 V Recorder output 1 channel 0 1 V Service For service use only Remote Digital control signals Mains Supply voltage grounded On off Instrument on off switch 36 Monitor UVis 920 11 0031 12 Edition AC Reference information 6 Analog out connector Connect a chart recorder to the 6 pole MiniDIN female Analog out 0 1 V connector 240 A 3 a N e m Pin Signal Function 1 0 1 V signal 2 Ground 3 6 Not used Table 6 1 Analog out connector PIN table Remote connector UVis 920 can be controlled via the 9 pole D Sub female Remote connector Pin Signal Function 5 1 5V Out Maximum load 50 mA 9 4 2 Not used 8 3 7 2 3 Not used 6 1 4 Auto Zero In Negative edge zeroes AU value 5 Gnd Common ground for all signals 6 Error Out Goes low at error Goes high again when OK is pressed 7 State Out 0 UVis 920 is in Run mode 1 UVis 920 is in End mode 8 Run Stop In Toggles the system between Run and End Negative edge selects Run mode starts Positive edge selects End mode stops 9 Event Mark In Negative edge makes an event mark on recorder Table 6 2 Remote connector PIN table Discrete O s in the remote connector uses 5 V TTL signals Monitor UVis 920 11 0031 12 Edition AC 37 6 Reference information 38 6 1 2 Monitor principle A Xe
25. osed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning MMM the decommissioning of equipment Contents Monitor UVis 920 11 0031 12 Edition AC Contents Introduction LI GENOM aste e EE RE 7 L S AIO E 8 Installation 21 UNPACKING 2 2 General precautions 2 3 Installing the cell holder svcccuuuummmmmmmuun tt teaa tt tasasen 10 2 4 Installing the flow cell cccccuommmuuuusme tmm tst tanassa 11 2 5 Connecting electrical cables ccucuuommuumummmmmumum stt 16 Operation 3 1 Menu selection and settings cccooiouiniit tt tettu KEE E 3 3 Main Menu OVETVIEW EEN 3 4 Setting wavelength steet asa te east nassen 35 Seting larnp OMOR eeben 3 6 Reading absorbance values Sf AUIEY 1 K o E 3 8 SE ee SLU RE 5 9 SILE AE KON A EN 3 10 UV CelliCallDBOtiON isma mass e aE mme 3 11 Changing flow ce 3 12 Restart after power failure Maintenance 41 Periodic maintenance sssini 29 4 2 Cleaning the instrument housing seeccuccomiomuumuuneses enaa 29 4 3 Cleaning the flow cell and optical fiber connectors 29 Troubleshooting 51L GSMS eisereen 31 5 2 Faultsand ACTON Sieros 32 5 3 ENIKOMIMMESSA OSS masamemarsa auma eegenen 35 Contents Reference information 6 1 6 2 6 3 6 4 6 5 6 6 6 7 6 8 DOSED LON E ER EUR
26. ow cell Dirt in the flow cell or fiber connectors Check with water i noisy the signal is still If there is a lot of air in the water degas the buffer continuously helium sparging is recommend Check the connections of the UV cell optical fibers Clean the UV cell see 4 3 Cleaning the flow cell and optical fiber connectors Ghost peaks Air in the eluents Dirt or residues in the flow path from previous runs Residues in the column from previous runs Degas if necessary we recommend helium sparging Clean the flow cell and flow path Clean the column in accordance with the column instructions Error in external chart recorder The recorder not properly set up Monitor UVis 920 not properly set up Check the chart recorder in accordance with its manual Test the recorder function by selecting recorder test according to 6 5 3 Check analog output Table 5 1 Faults and corrective actions Monitor UVis 920 11 0031 12 Edition AC 33 5 Troubleshooting 34 5 3 Error messages If the suggested actions do not correct the fault call GE Healthcare Message Description Action Cell fiber fail Check the connections of the UV cell optical fibers Check the liquid Ensure that there are no air bubbles in the system Clean the UV cell see section 4 3 Cleaning the flow cell and optical fiber connectors o filter inserted Insert a wavelength filter unit in the
27. quipment Monitor UVis 920 11 0031 12 Edition AC 31 5 Troubleshooting 5 Troubleshooting 5 1 General When contacting GE Healthcare for support state the software version of the instrument which is shown in the check menu 1 Select menu Check press OK Software Version 2 Select menu Software Version V1 00 00 WARNING The instrument must not be opened by the user It contains high voltage circuits which can give a lethal electric shock WARNING When using hazardous chemicals make sure that the entire flow cell has been flushed thoroughly with bacteriostatic solution for example NaOH and distilled water before service and maintenance This symbol indicates that the waste of electrical and electronic eguipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment az 32 Monitor UVis 920 11 0031 12 Edition AC 5 2 Faultsand actions If the suggested actions do not correct the fault call GE Healthcare Troubleshooting 5 Fault Possible cause Corrective action No text on the display No power to the monitor Check that the mains cable is connected and the power switch is in ON position 1 Noisy UV signal signal drift or instability The buffer may be impure There may be air in the fl
28. ril 2005 751 84 Uppsala All goods and services are sold subject to the terms and conditions of sale of Sweden the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Pacific Tel 85 65 62751830 Fax 85 65 62751829 e Australasia Tel 61 2 8820 8299 Fax 61 2 8820 8200 e Austria Tel 01 57606 1613Fax 01 57606 1614 e Belgium Tel 0800 73 890 Fax 02 416 8206 e Canada Tel 1 800 463 5800 Fax 1 800 567 1008 Central East amp South East Europe Tel 43 1972 720 Fax 43 1972 722 750 e Denmark Tel 45 70 25 24 50 Fax 45 45 16 2424 e Eire Tel 1 800 709992 Fax 44 1494 542010 e Finland amp Baltics Tel 358 9 512 3940 Fax 358 9 512 39439 e France Tel 01 69 35 67 00 Fax 01 69 41 98 77 e Germany Tel 0800 9080 711 Fax 0800 9080 712 e Greater China Tel 852 2100 6300 Fax 852 2100 6338 e Italy Tel 02 26001 320 Fax 02 26001 399 e Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 e Korea Tel 82 262013700 Fax 82 2 6201 3803 e La
29. rument at the mains switch on the rear panel 2 The instrument starts and displays the main operating window For optimum performance 30 minutes warm up time is recommended UVis 920 can at any time be switched off by the mains switch on the rear panel All set values are retained in the instrument 3 3 Main menu overview Main operating menu The main operating menu main window shows the wavelength the cell path length the absorbance value and the mode of operation The menu is reached from any other menu by pressing Esc repeatedly Wavelength in nanometer Cell Path Length in millimeter A 215nm CL 10 00mm 0 00000AU RUNNING Absorbance value in AU Status mode of operation Fig 3 7 Main operating menu main window The display always shows the mode of operation STANDBY or RUNNING in main operating window Setup menu Setup of analog out averaging time serial number etc Use the arrow keys to reach setup menu Check menu Check internal operating values Use the arrow keys to reach check menu Monitor UVis 920 11 0031 12 Edition AC 23 3 Operation A 215nm CL 10 00mm 0 00000AU STANDBY 24 3 4 Setting wavelength The instrument can measure absorbance at wavelengths between 200 700 nm by changeable filter units The wavelength currently used is shown in main operating menu When no filter is inserted the display shows A nm At delivery a dummy filter is in place for protection It
30. s and salt buffers commonly used in chromatography of biomolecules except 100 Ethyl acetate 100 Hexane and 100 Tetrahydrofuran THF or 15 THF in acetonitrile Tubing connections TC50 connector See pressure flow graphs in UV 900 industrial flow cells instructions 28 4051 40 Monitor UVis 920 11 0031 12 Edition AC Reference information 6 6 8 Accessories and spare parts CAUTION Only spare parts approved or supplied by GE Healthcare may be used for maintaining and servicing the monitor and the flow cells UV Monitors Item Ouantity Code no Monitor UVis 920 complete without flow cell and 1 11 0007 54 without filter Flow cells Item Ouantity Code no UV Flow Cell 2 mm incl optical fibres 1 18 1111 10 UV Flow Cell 10 mm incl optical fibres 1 18 1111 11 UV cell 1 2 5 mm KTApilot incl optical fibres 1 11 0008 50 ndustrial flow cell 8 mm 1 18 1134 56 ndustrial flow cell 1 1 18 1174 09 Fiber Kits for the Industrial flow cells Short optical fibre Kit 20 cm 1 18 1134 85 Long optical fibre Kit 50 cm 1 18 1134 86 Filter units Item Ouantity Code no 215 nm 1 11 0007 33 260 nm 1 11 0007 34 280 nm 1 11 0007 35 405 nm 1 11 0007 36 546 nm 1 11 0007 37 Empty filter holder for custom filter 1 11 0007 38 Signal cables Item Quantity Code no Signal cable for recorder Length 1 5 m al 18 1110 64 Monitor UVis 920 11 0031 12 Edition AC 51 6 Re
31. s arrow down key Parameter Set Averaging Time 2 56s lt 2 56 gt Current value New value to be set Fig 3 5 Example value to be set The text or numerical value displayed is accepted by pressing the OK key To cancel press the Esc key If the new value to be set is within brackets it is possible to select between a number of preset values No brackets make it possible to select step by step within a preset range for example 0 999 To set numerical values faster press arrow up key continuously The display shows 1 2 3 10 20 30 100 200 300 1000 2000 3000 It is possible to select decade by pressing arrow down key continuously Each character steps down to zero and then the cursor moves to left and stops at the desired decade when the key is released Error messages If an error is occurred the display shows an error message Error 109 No filter inserted Lo Error type User information Fig 3 6 Example of error messages To handle problems through the display interface even if an error message is indicated it is possible to return to the previous display Press OK or Esc If the error remains a new display with the same message returns 10 seconds after the last display update See chapter 5 Troubleshooting for more information Monitor UVis 920 11 0031 12 Edition AC Setup Check Operation 3 3 2 UVis 920 on off 1 Switch on the inst
32. s the full scale value on the recorder the signal will instantly drop to 0 V and give an accurate display of the peak starting from this position Event mark Event marks can be set for example when the sample is injected and are displayed as spikes on the chart recorder 1 Press event mark key The spikes are 10 of the full scale of the chart recorder which corresponds to 0 1 V Monitor UVis 920 11 0031 12 Edition AC Set Averaging Time 2 56s Set Averaging Time 2 56s lt 2 56 gt Monitor UVis 920 11 0031 12 Edition AC Operation 3 3 8 2 To filter the noise in the UV signal a moving average filter is used The averaging time is the time interval used for calculating the moving average of the absorbance signal A long averaging time will smooth out noise efficiently but it will also distort the peaks Peaks narrower than the minimum peak width value may be distorted Because of this the averaging time should be as short as possible see the table below On delivery the averaging time is set to 2 56 s Setting averaging time filtering noise 1 Select menu Set Averaging Time press OK 2 Usethearrow keys to set the value Use the fixed values between 5 12 and 0 08 seconds Averaging time s Time constant s Min peak width approximate at half height s 5 12 2 0 32 2 56 1 0 16 1 28 0 5 8 0 0 64 0 2 3 2 0 32 0 1 1 6 0 16 0 05 0 8 0 08 0 03 0 5 Table 3
33. sing hazardous chemicals make sure that the entire system has been flushed thoroughly with bacteriostatic solution e g NaOH and distilled water before service and maintenance Monitor UVis 920 11 0031 12 Edition AC 29 4 Maintenance CAUTION Do not allow solutions containing dissolved salts proteins or other solid solutes to dry out in the cell Do not allow particles to enter the flow cell as damage to the flow cell may occur 30 Monitor UVis 920 11 0031 12 Edition AC Maintenance 4 4 3 1 Cleaning the flow cell 1 Connecta syringe to the inlet of the flow cell and squirt distilled water through the cell in small amounts Then fill the syringe with a 10 surface active detergent solution like Decon 90 Deconex 11 RBS 25 or equivalent and squirt five times 2 After five squirts leave the detergent solution in the flow cell for at least 20 minutes 3 Pump the remaining detergent solution through the flow cell 4 Rinse the syringe and flush the cell with distilled water 10 ml 4 3 2 Cleaning the optical fiber connectors When required wipe the optical fiber connectors with 30 isopropanol on lens paper 4 4 Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer Eo for information concerning the decommissioning of your e
34. strument uses high intensity ultra violet light Do not disconnect the optical fibers while the monitor is running 2 Remove the two rubber protective caps from the optical fiber receptacles on the right side of the housing a Ar Black shrinking tube s N Rubber sleeve Protective cap Fig 2 5 Connection of optical fibers to Monitor UVis 920 Monitor UVis 920 11 0031 12 Edition AC Installation 2 3 Connect the two optical fibers to the housing by carefully inserting them into the sockets and tightening the nuts fingertight using the fiber detachment tool supplied Do not overtighten Note The optical fiber end with black shrinking tubing shall be connected to the rear socket connector on the side of UVis 920 Fiber detachment tool CAUTION Do not twist the optical fibers during tightening 4 Slide the rubber sleeves on the two optical fibers onto the connectors Make sure that the sleeves are pushed tight to the housing to prevent dust fluid or light entering the connection 5 Remove the red protective caps from the inlet and outlet of the flow cell and connect the tubing with 1 16 fingertight connectors Fig 2 6 Connecting tubing to flow cell 6 Mount the cell holder cover The cover is a simple push fit onto the cell holder Two small lugs on the cover fit in holes at the front and rear of the cell holder The cover is then lowered over the cell holder Fig 2 7 Mounting o
35. th water at 25 Liguid temperature range 4 to 40 C Optical path length 2 and 10 mm Cell volume 2 mm cell 2 ul 10 mm cell 8 ul Wetted materials PTFE polytetrafluoroethylene PEEK polyetheretherketone Titanium palladium alloy Quartz synthetic fused silica pH stability range 1 13 13 14 lt 1 days exposure Chemical resistance The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules Tubing connections UNF 10 32 fingertight connectors for capillary tubing with 1 16 outer diameter UV cell 1 2 5 mm KTApilot Recommended max flow rate 800 ml min Max pressure 2 MPa 20 bar 290 psi Backpressure Max 0 05 bar at 400 ml min with water at 25 Liguid temperature range 4 to 40 C Optical path length 1 2 and 5 mm Cell volume 300 ul Wetted materials PEEK polyetheretherketone Titanium palladium alloy FFKM perflorelastoner or EPDM Quartz synthetic fused silica pH stability range 1 13 13 14 lt 1 days exposure Chemical resistance The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules except 100 Ethyl acetate 100 Hexane and 100 Tetrahydrofuran THF Tubing connections 5 16 Monitor UVis 920 11 0031 12 Edition AC
36. tin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 e Middle East amp Africa Tel 30 210 96 00 687 Fax 30 210 96 00 693 e Netherlands Tel 0800 82 82 82 1 Fax 0800 82 82 82 4 e Norway Tel 47 815 65 777Fax 47 815 65 666 e Portugal Tel 21 417 7035 Fax 21 417 3184 e Russia amp other C I S amp N I S Tel 7 495 956 5177 Fax 7 495 956 5176 Spain Tel 902 11 72 65 Fax 935 94 49 65 e Sweden Tel 018 612 1900 Fax 018 612 1910 Switzerland Tel 0848 8028 10 Fax 0848 8028 11e UK Tel 0800 515 313 Fax 0800 616 927 USA Tel 1 800 526 3593 Fax 1877 295 8102 imagination at work 11 0031 12 AC 06 2007 EE Elanders sterv la 2007
37. to the instrument is interrupted the instrument automatically restarts itself and displays the main operating menu All set values are retained in the instrument but the instrument starts with the lamp switched off standby Monitor UVis 920 11 0031 12 Edition AC Maintenance 4 Maintenance gt N N 4 WARNING Always disconnect the power supply before attempting to replace any item on the instrument during maintenance WARNING Only spare parts that are approved or supplied by GE Healthcare may be used for maintaining or servicing the instrument WARNING Remove liquid or dirt from the system surface using a cloth and if necessary a mild cleaning agent 4 1 Periodic maintenance UVis 920 does not require any periodic maintenance 4 2 Cleaning the instrument housing e Wipe the instrument housing regularly with a damp cloth Do not allow spilt liquid to dry on the instrument e Remove dirt from the surface using a cloth and a mild cleaning agent e Let the instrument dry completely before using it 4 3 Cleaning the flow cell and optical fiber connectors A clean flow cell and optical connectors are essential for ensuring the correct operation of the UV monitor WARNING When using hazardous chemicals make sure that the entire flow cell has been flushed thoroughly with bacteriostatic solution for example NaOH and distilled water before service and maintenance WARNING When u
38. utozero function sets the detected absorbance to zero when the AUTOZERO key is pressed Autozero is recommended before a sample is injected 1 Press Autozero The absorbance value is then shown in main operating menu Monitor UVis 920 11 0031 12 Edition AC 25 3 Operation Analog Out 2 000AUFS 10 Set Range 2 000AUFS Set Range 0 050AUFS lt 0 050 gt Set Zero Level 10 N Signal to recorder V Measured absorbance level AU Range Overrange 1V 26 3 8 Setupmenu 3 8 1 Setting analog output to an external chart recorder Setting range and zero UVis 920 displays the measured absorbance value as an analog voltage in the recorder connector The output from the instrument is always 0 1 V but the absorbance value for full scale deflection AUFS and the zero absorbance level on the recorder can be set 1 Select menu Analog Out press OK 2 Select menu Set Range press OK The range is the full scale absorbance range for the chart recorder 1 V 3 Usethearrow keys to set the absorption range for recorder output in AUFS press OK Only fixed steps between 5 0 AUFS and 0 001 AUFS can be set 4 Select menu Set Zero Level press OK The zero level is where on the paper the 0 AU value will be positioned Values 0 99 5 Use the arrow keys to set the value and press OK The instrument has an automatic overrange function If the monitor signal reache
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