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        Single Channel Heater Controller User`s Manual
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1.   is to pre heat the solution with an in line heater in conjunction  with platform heating     Operating With Other Eguipment    The TC 324B can be used to power other Warner eguipment such as the  SH 27B and SF 28 Slow Flow solution heaters  In addition  the controller  can be used as an independent device to power third party eguipment as  long as the following considerations are met     Load Resistance    The TC 324B heater controller will work well with many other  heating devices To obtain maximum heating power  the resistance  of the heating element should be between 8 12 Q  but any element  that works at a maximum of 12 V and 1 5 A will also work with  the TC 324B     Thermistor Considerations   The TC 324B heater controller was designed to accommodate Unical  thermistors from Thermometrics  Edison  New Jersey  This family of  thermistors can be interchanged without recalibration of the    instrument  The nominal resistance of Unical thermistors is  10 0 KQ at 25   C     Other thermistors may be used with the TC 324B if the nominal  resistance also is 10 0 kQ at 25   C The use of thermistors with other  characteristics may not be possible  or will reguire recalibration of  the TC 324B     Publication 5700 001 REV C    s    Single Channel Heater Controller    Warner Instruments    Operation    The main use of a heater control device such as the TC 324B is to maintain  a constant bath temperature with minimum deviation from a set tempera   ture This is usually achieved b
2.  It is normal for some overshoot to occur while  the temperature is stabilizing  and these features prevent the temperature  from reaching very high or very low levels during this time     Manual Mode    In manual mode  the MANUAL VOLTAGE control  Item D  is used to set the out   put voltage to a fixed value  The voltage setting can be viewed with the  METER when the METER SELECTOR switch  Item E  is set to Output Voltage  In  manual mode  the SET TEMPERATURE control  Item C  is disconnected  as is  the feedback system of the TC 324B  The instrument now operates as a  0 12 VDC power supply that can provide up to 1 5 A of current to a device   Use this mode with heater systems that will operate properly with a fixed  voltage and lack feedback thermistors     Publication 5700 001 REV C    5    Single Channel Heater Controller    Warner Instruments    Operation    Connection To External Devices    In Line Heaters   The SH 27A and SF 28 in line solution heaters connect directly into  the TC 324B Output connector  Item H   For these devices the tem   perature of the solution is monitored by a thermistor placed at the  outflow end of the in line heater  This is the T1  CONTROL  thermistor   The T1 RECORDER OUTPUT BNC  Item J  on the TC 324B front panel can  be used to measure the temperature of this thermistor at a scale  factor of 100 mV   C A second thermistor  plugged into the T2 INPUT  on the instrument rear panel  can be used to measure the  temperature at any other point of i
3.  Nomenclature    Text conventions    This manual refers to amplifier controls at two functional levels  specific  controls and settings of these controls  To minimize the potential for con   fusion  we have employed several text conventions which are specified  below  Since our goal is to provide clarity rather than complexity  we  welcome any feedback you may wish to provide       Warner Instrument product numbers are presented using bold type     References to controls are specified using SMALL CAPS   e References to control settings are specified using italic type     e Special comments and warnings are presented in Idata tao    Any other formatting should be apparent from context     Publication 5700 001 REV C    s    Single Channel Heater Controller    Warner Instruments    Control Description    Front panel    A schematic of the face panel of the TC 324B is shown below  Important  components are identified by letter  B H  J   Please refer to this diagram as  an aid in identification of described components        WARNER  TC 324B     Manual Voltage mi   INSTRUMENT   0 To 12 Volts      CORPORATION    up  HEAT    O Down    SET  O ewe SnATURE    RECORDER OUTPUTS o  T1  Control  T2  Monitor  T 1  Control  T 2  Monitor  OUTPUT    OUTPUT OUTPUT set Temp Output        GM AUTO  Biol    100mV     of  AUTOMATIC TEMPERATURE O_O          Power Switch  Located directly under the Warner logo  Supplies power to the  TC 324B     Output Switch  Item B    Supplies or removes power to the 
4. OUTPUT CONNECTOR  Item H   Selects  between Automatic Mode  Manual Mode  or center off  The  associated green LED is lit when output power is on  auto or  manual mode selected      Set Temperature Control  Item C    Used to adjust the set point of the automatic control system The  associated green LED is on when the controller is set to Automatic  Mode on the OUTPUT SWITCH  item B      Manual Voltage Control  item D    Used to manually set the voltage output to the heater device The  associated green LED is on when the controller is set to Manual  Mode on the OUTPUT SWITCH  item B      Meter Selector Switch and Meter  Item E     SELECTOR SWITCH selects the parameter that the METER  Item F  will  display  Options include Set Temperature  TI  Contro   T2  Monitor    and Output Voltage     Publication 5700 001 REV C    2    Single Channel Heater Controller    Warner Instruments    Control Description  Conto     Meter  Item F   Displays the parameter selected by the METER SELECTOR SWITCH  Item E    The METER also provides an indication that the MAIN POWER is 07     Loop Speed Switch  Item G    Sets the feedback loop speed for the heater sensor system when the  device is used in automatic mode  Options include fast  medium  and  slow  Optimally set to fast for most applications  but can be set to  medium or slow for systems with longer thermal delay characteristics     Output Connector  Item H    Connects the TC 324B to attached heater blocks and sensor  thermistors  When used wit
5. RATURE control  Item C  to the desired temperature  Observe  setting On METER     Set the LOOP SPEED switch  item G  to fast   Switch the METER SELECTOR switch  Item E  to 77  Control      Set the OUTPUT switch  Item B  to auto The OUTPUT LED will be lit to  indicate that power is being applied to the heater  Observe the METER  to assure that the temperature is approaching the set temperature   The HIGH and Low LED s indicate the direction of heating control     NOTE  If the system is setup properly  this is all that is needed    to maintain a constant temperature in the chamber        10  Allow time for the system to stabilize at the desired temperature  Set    the METER switch  Item E  to 72  Monitor  This will display the read   ing of the monitor thermistor submerged in solution  This reading  will most likely be lower than the platform set temperature due to  the thermal gradient between the platform and the solution  The tem   perature at the sample  T2 thermistor reading  can be adjusted to the  desired setting by increasing the SET TEMPERATURE setting as described  in step 6 above  This is an example of static solution warming and   is the simplest form of temperature control     Publication 5700 001 REV C    3    Single Channel Heater Controller    Warner Instruments    Setup  Cont d     Temperature control is more complex in a system where solu   tion continuously flows across the bath The best method for  providing stable temperature control for a flowing solution 
6. Single Channel  Heater Controller    Model TC 324B       WARNER  ISTRUMEN    AUTOMATIC TEMPERATURE CONTROLLER       WARNER  A INSTRUMENTS    A Harvard Apparatus Company    Publication 5700 001 REV C    WEEE RoHS Compliance Statement    EU Directives WEEE and RoHS    To Our Valued Customers     We are committed to being a good corporate citizen  As part of that commitment   we strive to maintain an environmentally conscious manufacturing operation  The  European Union  EU  has enacted two Directives  the first on product recycling   Waste Electrical and Electronic Equipment  WEEE  and the second limiting the use  of certain substances  Restriction on the use of Hazardous Substances  RoHS    Over time  these Directives will be implemented in the national laws of each EU  Member State     Once the final national regulations have been put into place  recycling will be offered  for our products which are within the scope of the WEEE Directive  Products falling  under the scope of the WEEE Directive available for sale after August 13  2005 will  be identified with a    wheelie bin    symbol     Two Categories of products covered by the WEEE Directive are currently exempt  from the RoHS Directive   Category 8  medical devices  with the exception of  implanted or infected products  and Category 9  monitoring and control instruments   Most of our products fall into either Category 8 or 9 and are currently exempt from  the RoHS Directive  We will continue to monitor the application of 
7. h Series 20 chambers and platforms the  CC 28 heater cable connects here The in line solution heaters also  connect here     Thermistor Recorder Outputs  Items J    BNC connectors are provided to send thermistor readings  T1 and T2  output signals  to a data acguisition system or chart recorder  Outputs  are calibrated to 100 mV C     Up   Down LED   Item K     Red and yellow LED s indicate relative power applied to heater     Publication 5700 001 REV C    s    Single Channel Heater Controller    Warner Instruments    Control Description    Rear panel       120 VAC to 220 VAC Conversion    ATTENTION    PLEASE READ BEFORE APPLYING POWER TO YOUR UNIT        The unit has been set to be used with 120 VAC     If the VAC needs to be changed to 220VAC  enclosed you will find a kit   power cord  1  and fuses  2   to be used to convert the unit from 120VAC    to 220 VAC     The unit uses only one fuse  the second one is sent as spare     Follow these instructions to change the unit s  from 120VAC or 220VAC                                                                                Power Entry Module  Fuse holder side view   Push out drawer to access  the Spare Fuse  i       Active  Fuse       Depending on AC Voltage being  used  turn VAC selector switch  to 110VAC or 220 VAC       Publication 5700 001 REV C    Carefully  pry open the fuse holder from the inside  by using a small flat screwdriver   Replace fuse according to voltage being used     Item   64 0100 Model TC 324B use   F
8. ii 11  Specifications  anali 11  Accessories and replacement partsS                 iii 11  CC 35 Connector pin designations                  iii 13  Warranty and Service       cccccsssessscssssssnssssscsessssssseeassssssseeaseesees 13  Warranty                   13    Service  NOES    LL AUE OLA nda 13            Single Channel Heater Controller    Warner Instruments    Introduction    The TC 324B Single Channel Automatic Heater Controller from Warner  Instruments is a versatile and simple to use thermal control device   Although designed for use with Warner Series 20 chambers or in line solu   tion heaters  this instrument can be easily adapted for use with custom built  equipment according to the requirements of the user     Total automatic control of heating power is provided in automatic mode   while manual control is available in manual mode  A loop speed selector is  used to optimize the response of the system to accommodate the thermal  delay characteristics intrinsic to the environment     Features of the TC 324B include     Dual thermistor design     Automatic and manual modes   e Selectable loop speed optimizes system speed and stability    e Simultaneous monitoring of system temperature and a separate  point of interest    e Optimized for use with Warner perfusion and imaging chambers  e Compatible with SH 27B In line Solution Heater      Provides 18 W of heating power into a 80 load    THIS EQUIPMENT IS NOT DESIGNED NOR INTENDED    FOR USE ON HUMAN SUBJECTS      
9. ilities using 220 240 V line  voltages   If the replacement fuse also fails  please call  Warner Instruments for assistance     B  Occasionally  a knob on the front panel will loosen after long  use These are  collet  style knobs and are tightened with a  screw located under the knob cap To gain access to the adjust  ment screw  pry the cap off with a thin bladed screwdriver or  similar tool     C  Should service be required  please contact the factory The  problem may often be corrected by our shipping a replacement  part  Factory service  if required will be expedited to minimize  the customer inconvenience     Publication 5700 001 REV C     gt     Single Channel Heater Controller    Warner Instruments    Appendix  Conto     D  Instruments are inspected immediately upon receipt and the  customer is notified if the repair is not covered by the warranty   Repairs can often be completed in 1 2 days from our receipt of  the instrument     E  If factory service is required  please observe the following  instructions     1  Package the instrument with at least 3 inches of  cushioning on all sides  Use the original shipping carton  if it is available     2  Insure the shipment for its full value     3  Include with the shipment an explanation of the  problem experienced     IMPORTANT   CUSTOMERS OUTSIDE OF THE U S   Please be sure to  contact us before return shipping any goods  We will provide instruc     tions so that the shipment will not be delayed or subject to unneces     sa
10. ing the  bath temperature  The connector end plugs directly into the TC 324B  OUTPUT CONNECTOR  Item H      Assembly instructions    1  Assemble the Series 20 chamber as described in the instructions  supplied with the chamber     2  Connect the outboard end of the CC 28 cable to the Series 20  platform     a  First  attach the two blue 3 pin connectors on the cable  to the heater resistors on the platform     Publication 5700 001 REV C    2    Single Channel Heater Controller    Warner Instruments    Setup    3     4     Vi    6     7   8   9     b  Next  insert the larger thermistor  white wires or white  band  on the cable into the small hole in the side of  the platform     HINT  To assure good thermal contact between the    thermistor and platform  place a drop of mineral or       immersion oil in the thermistor hole prior to insertion     Place the small bead thermistor into the chamber reservoir     NOTE  You will most likely need to restrain the cable and  thermistor bead as the bead must be completely submerged  for accurate temperature measurements        Fill the solution bath to the desired level     HINT  For accurate temperature control  protect the microscope  stage from drafts The solution surface offers a large surface area  for heat exchange and minimizing air flow in the immediate       vicinity will ease your temperature control requirements     Turn the controller POWER 07     Switch the METER SELECTOR switch  Item E  to Set Temp and adjust the  SET TEMPE
11. nterest     Using Other Devices    A CC 35 cable assembly is required for connecting other external  devices to the TC 324B heater controller  See the Appendix for  CC 35 pin designations     Publication 5700 001 REV C    5    Single Channel Heater Controller    Warner Instruments    Appendix    Specifications   Maximum Output Voltage  Maximum Output Current  Maximum Load Resistance  Manual Voltage Range  Maximum Output Power  Power Requirements  Power Fuse  3AG Size     Front Panel Recorder Outputs  Rear Panel Input    Temperature Range  Meter Display  Meter Readouts    Enclosure    12 VDC   1 5A   8 00   0 0 to 12 0 VDC   16 W into a 9 Q load   100 130 or 200 260 VAC  50 60 Hz    0 5 A Slo Blo for 100 130 VAC  0 25 A Slo Blo for 200 260 VAC    T1  control   T2  monitor   BNC reporting 100 mV C    T2 thermistor  monitor   BNC input calibrated at 10 0 KQ at 25   C    Ambient to 50   C  3 5 digit LED display of   C or V    Set temperature   T1  control  temperature  T2  monitor  temperature  Output voltage  to heater     17 0 x 3 5 x 12 0 in  W x H x D     Accessories and Replacement Parts    CC 28  CC 35    Publication 5700 001 REV C    Cable assembly for Series 20 heater platforms  Cable assembly with unterminated outboard ends    Single Channel Heater Controller    Warner Instruments    Appendix  Conto     Color cn LL    ETRE TT I  2   Red   Heaterpomer      T2  Monitor   e No connection         Thermistor  common N C     N C         Control  Thermistor T1 N C   Heater  gro
12. or 120 VAC  0 50 Amp   5x20mm Slow Blow  For 220 VAC  0 25 Amp   5x20mm Slow Blow             2    Single Channel Heater Controller    Warner Instruments    Control Description  Conto     T2 Input    Used as an optional thermistor input for those configurations where  a T2  Monitor  thermistor is not included in the normal setup  such  as with an In line Heater   Sensed thermistor readings can be dis   played on the METER and sent to a recording device via the standard  T2 OUTPUT BNC on the front panel     NOTE  Do not use this input if a T2  Monitor  thermistor is currently       connected to the OUTPUT CONNECTOR  Item H  on the front panel     Ground Terminals    Provides separate connections for chassis and circuit ground  Banana  jacks are bridged when shipped from the factory     The TC 324B can be used to drive the heater blocks for Warner Series 20  chambers or for providing power to other devices such as our In line  Solution Heaters     NOTES     a  Please refer to the front panel schematic on page 6 for  orientation     b  Turn both the SET TEMPERATURE control  Item C  and the       MANUAL VOLTAGE control  Item D  fully counterclockwise to zero     Setup       Series 20 Chamber Setup    The CC 28 cable assembly is used to connect Series 20 chambers to the  TC 324B heater controller  The chamber end of the cable includes two  connectors that attach to the resistive heaters in the heater platform Two  thermistors are also provided  one for feedback and one for monitor
13. or electrical  eguipment for measurement and  laboratory use    Class I    TC 324B and TC 344B    1  the undersigned  hereby declare that the eguipment specified    above conforms to the above Directive s  and Standard s      Publication 5700 001 REV C    Place  Hamden  Connecticut  USA    i  Signature  kal   Full Name  Ralph Abate  Position  Business Manager       
14. ry expense in clearing U S  Customs        Publication 5700 001 REV C    Single Channel Heater Controller    Warner Instruments    Appendix    CE Certifications    EMC    Declaration of Conformity    CE MARKING  EMC     Application of Council Directive  89 336 EEC    Standards To Which  Conformity Is Declared     Manufacturer   s Name   Manufacturer   s Address     Equipment Description     Equipment Class     Model Numbers     EN55022 Class A  EN61000 3 2  EN61000 3 3  EN50082 1 1992  EN61000 4 2  EN61000 4 3  ENV50204  EN610000 4 4  EN610000 4 8  EN610000 4 11    Warner Instrument Corp   1125 Dixwell Avenue  Hamden  CT 06514   Tel   203  776 0664    Heater Controller    ITE Class A    TC 324B and TC 344B    1  the undersigned  hereby declare that the eguipment specified    above conforms to the above Directive s  and Standard s      Publication 5700 001 REV C    Place  Hamden  Connecticut  USA        4 4      Signature  be JA ar      Full Name  Ralph Abate  Position  Business Manager       Single Channel Heater Controller    Warner Instruments    Appendix  Conto     LVD    Declaration of Conformity    CE MARKING  LVD     Application of Council Directive  73 23 EEC    Standards To Which  Conformity Is Declared     Manufacturer   s Name   Manufacturer   s Address     Eguipment Description     Eguipment Class     Model Numbers     EN 61010 1 1993    Warner Instrument Corp   1125 Dixwell Avenue  Hamden  CT 06514   Tel   203  776 0664    Heater Controller   Safety reguirements f
15. the RoHS  Directive to its products and will comply with any changes as they apply       Do Not Dispose Product with Municipal Waste      Special Collection Disposal Required       2    Single Channel Heater Controller    Warner Instruments    Table of Contents    SUBJECT PAGE                           INTO UCI ci   2  Nomenclature      Text Conventions  Control Description         ccocaccccononon  Front panel  Arv cages iaia  IS AAA  Output Switch  Item B   Set Temperature Control  Item C     o cocininninnicinnnmmmmmrrrer  Manual Voltage Control  item D               iii  Meter Selector Switch and Meter  Item E     Meter  Item  Plica Rella  Loop Speed Switch  Item G        ssssssssssssssssssssssrrrrssssssrrrrrressnnssrrrneennnnsnnrreneee  Output Connector  Item H   Thermistor Recorder Outputs  Items J      ssssssssssesssssssssssssssssessesssssssssssseenees  Up Down LEDS  Hno da 4  Rear Panel                             AC Conversion     T2 INput                Ground Terminals  QCD tanel linate drei iti 6  Series 20 Chamber Setup    6  Assembly instruclioNs        pi eeta ua ala 6  Operating With Other Equipment                         mmmmmmmnerannann 8  Load Resistance  Thermistor Considerations                                riee 8  Operation aida is 9  Automatic MOde                   ie 9  Manual  MODOS  dida 9  Connection to External Devices                   iin 10  In Line Heaters  Using Other DEVicesS                            rrierierie ieri neon 10  Appendix    i
16. und    Monitor  Thermistor T1    Heater    Publication 5700 001 REV C         Single Channel Heater Controller    Warner Instruments    Appendix    Warranty and service    Warranty    The model TC 324B is covered by our Warranty to be free from  defects in materials and workmanship for a period of two years from  the date of shipment  If a failure occurs within this period  we will  either repair or replace the faulty component s  This warranty does  not cover instrument failure or damage caused by physical abuse or  electrical stress  inputs exceeding specified limits      In the event that instrument repairs are necessary  shipping charges  to the factory are the customer s responsibility  Return charges will  be paid by Warner Instruments  Inc     Normal business hours are 8 30 AM to 5 30 PM  EST   Monday  through Thursday and 8 30 AM to 5 00 PM on Friday  Our offices are  located at 1125 Dixwell Avenue  Hamden  CT 06514  and we can be  reached by phone at  800  599 4203 or  203  776 0664  Our fax num   ber is  203  776 1278  In addition  we can be reached by e mail at  support warneronline com or through our Web page at  http   www warneronline com     Service notes    Please refer all questions regarding service to our Engineering  Department     A  If the instrument POWER light fails to light  check the fuse at the  rear panel  located in the black POWER INPUT MODULE   If the  fuse is found to be defective replace it with a 5x20 mm  0 5 A  slow blow fuse  0 25 A for fac
17. y using a heated chamber platform  an in line  solution heater  or a combination of both     Automatic Mode    In Automatic Mode  the TC 324B maintains the temperature of the con   nected heater thermistor system at the value set by the user The selectable  LOOP SPEED control  Item G  is used to adjust the speed of the feedback loop  in the heater thermistor system  which controls the rate of change of its  output voltage  Non Warner heater systems with feedback thermistors can  be used in automatic mode provided they are compatible with the  TC 324B reguirements     LOOP SPEED is normally set to fast to provide the shortest time between the  application of power to the heater element and the sensing of temperature  at the thermistor  For heater thermistor systems with long response times   the fast setting will cause the measured temperature to overshoot the tar   get by a large amount resulting in system oscillation  For this condition  try  medium or slow settings to find the optimum feedback rate     If the temperature of the CONTROL THERMISTOR is more than 0 5   C above the  set temperature  the voltage applied to the heater element will be immedi   ately set to 0 V  removing power to the heater  The output voltage will  remain at 0 V until the temperature of the CONTROL THERMISTOR becomes less  than 0 5      C above the set temperature  at which point power will be  gradually re applied to disallow the system temperature to fall too far below  the temperature set point 
    
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