YMC-Triart C18/C8 1.9 μm
Contents
1. column use pees Temperature range E 9 Regular use yecommended Upper limit nase pH1 7 70 C 1 12 aici pH7 12 50 C Column lifetime varies depending on conditions of use such as pH temperature and eluent composition In general using under higher temperature higher concentration of buffer salts additives and or lower concentration of organic solvents may result in shorter column lifetime When using the column under alkaline conditions for a long term a column should be used with a low concentration about 1 to 10 mM of organic buffer like triethylamine glycine etc at a lower temperature less than 30 C The eluent containing methanol would give longer column lifetime under alkaline condition compared to other organic solvents Aqueous or non aqueous solvents can be used as an eluent Repetitive replacement among solvents with large difference in polarities may result in degradation of column performance In general acetonitrile methanol and tetrahydrofuran THF are recommended for regular use Please also see above recommendation under alkaline eluent conditions When using THF as an eluent mind the solvent resistance of your system or tubing Especially PEEK parts are not suitable for use with THF Make sure of miscibility among the organic solvents and take care to prevent the precipitation of buffer salts additives to avoid over pressuring the column Use a sample diluent that has the same comp2. YMC HPLC COLUMN USER S MANUAL UHPLC Column YMC Triart C18 C8 1 9 um Column Care and Use Instructions 1 Introduction Thank you for purchasing a YMC Triart C18 C8 1 9 um column for ultra high performance liquid chromatography UHPLC YMC Triart 1 9 um is a UHPLC column based on 1 9 um hybrid silica gel YMC Triart 1 9 um exhibits higher efficiency and maintains efficiency over a wide range of flow rate and pressure compared to conventional 5 um 3 um columns Therefore YMC Triart 1 9 um enables ultra high throughput analysis and remarkable decrease in analysis time Additionally YMC Triart 1 9 um column offers easy method transfer between UHPLC and HPLC since it shows identical selectivity to conventional 5 um and 3 um YMC Triart columns with the same bonded phase YMC Triart 1 9 um is manufactured under highly controlled conditions and must pass a series of strict tests before being accepted for shipment Please refer to the column inspection report In order to ensure optimal performance and durability of the column please read these instructions carefully before using this column 2 Recommendations for column connections detector settings and data processing considerations O When connecting YMC Triart 1 9 um column to the LC system be careful with port depth of the column endfittings The PT or WT at the end of the product code indicates the style of column endfittings See below for details Consideration of connector and endf
3. ins or polysaccharides adsorbed onto the gel they are hardly removed even if solvents with high eluting ability are used In order to avoid contamination of the column by them conduct sample pretreatment carefully prior to introducing to the column YMC Co Ltd YMC Europe GmbH YMC America Inc YMC Karasuma Gojo Bldg Tel 49 2064 427 0 Fax 49 2064 427 222 Tel 1 610 266 8650 Fax 1 610 266 8652 284 Daigo cho Karasuma Nishiiru Gojo dori www ymc de info ymc de www ymcamerica com info ymcamerica com Shimogyo ku Kyoto 600 8106 Japan Tel 81 75 342 4515 Fax 81 75 342 4550 YMC India Pvt Ltd YMC Korea Co Ltd www ymc co jp sales ymc co jp Tel 91 120 4276020 25 Fax 91 120 427626 Tel 82 31 716 1631 Fax 82 31 716 1630 www ymcindia com sales ymcindia com www ymckorea com info ymckorea com UM111013AE
4. ittings Tubing and connector Column ate ae Port depth Style of endfittings im PT ca 2mm 0 09inch Parker style UPLC compatible WT ca 3 mm 0 13 inch Waters style R Port depth UPLC is a registered trademark of Waters Corporation O Tubing must have flat ends and must bottom out in the column endfitting In case tubing is not properly connected to the column a void appears between the bottom of the column endfitting and tubing And it will create a leak and or result in poor column performance e g peak tailing loss of theoretical plate number We have a connector with re adjustable ferrule which provides flawless sealing to 137 MPa 20 000 psi Please inquire us for the detail O0 Extra column volume has great impact on band spreading In order to minimize influence of band spreading on chromatographic performance LC system should be optimized as follows gt The shortest possible length of tubing with narrow inner diameters tubing less than 0 15 mm 0 006 inch I D is recommended should be used for connection from the injector to the column and from the column to the detector Make sure not to have void in connection gt Use a detector equipped with low volume flow sell designed for narrow bore column gt Use an injector for narrow bore column and low volume sample loop O0 YMC Triart 1 9 um gives high operating pressure compared to conventional 5 um 3 um columns Pay attention to the usable maximum
5. osition as the initial eluent conditions In order to prevent peak deterioration avoid using solution with stronger eluting ability than the initial eluent conditions as a diluent An eluent and a sample must be filtered out with a 0 2 um membrane to remove insoluble substances before use or injection 5 Column cleaning general method After using eluent not containing buffer salts additives 0 Flush the column with solution containing higher ratio of organic solvent for washing out the compounds that have a great capacity to retain in the column Usable concentration of organic solvent is up to 100 A cleaning solution containing THF may be effective when removing highly hydrophobic lipid soluble substances adsorbed onto the gel After using eluent containing buffer salts additives 0 0 Firstly replace with water organic solution that does not contain buffer salts additives A ratio of water to organic solvent should be set at the same proportions as an eluent Then flush the column in accordance with the method mentioned above The eluent containing about 50 mM or less buffer salts additives could be replaced directly with about 60 acetonitrile aqueous solution General proposals O Flushing with 100 water after using the column around the pH limit may shorten column lifetime Flush the column with water organic solution described above such as 60 acetonitrile aqueous solution O Once macromolecules like prote
6. pressure of tubing and LC system O0 A sampling rate and a detector response time constant should be optimized to detect earliest eluting sharp peak properly We would recommend using a sampling rate of about 10 points per second or higher and a detector response of 0 1 seconds or lower O Generally YMC Triart 1 9 um is designed for use of UHPLC system which has 60 MPa or higher pressure tolerance and reduced system volume 3 Shipping solvent 100 Acetonitrile Replace with this solvent for storage When replacing an eluent containing buffer or salts take extra care to prevent precipitation of salt YMC HPLC COLUMN USER S MANUAL 4 Precautions for use 0 0 0 The correct direction of solvent flow is indicated by an arrow on the column identification label Do not disconnect a column from LC system before the pressure drops to zero Note that abrupt pressure change or continuous use under extreme pressure may result in shorter column lifetime The maximum operating pressure for YMC Triart 1 9 um column is 100 MPa 14 500 psi Recommended flow rates for usage are 0 3 0 8 ml min for 50 X 2 0 mmI D and 0 6 1 8 ml min for 50 X 3 0 mmI D when using with acetonitrile aqueous as an eluent The column pressure differs depending on column length column temperature eluent composition and so on So adjust a flow rate that can provide optimal performance and practical pressure in use Recommendations of pH and temperature for
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