1 - Thermo Fisher Scientific
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1. Position Valve 1 Position A Pin 1 and Pin 2 Contact open Valve 1 Position B Pin 1 and Pin 2 Contact close Valve 2 Position A Pin 3 and Pin 4 Contact open Valve 2 Position B Pin 3 and Pin 4 Contact close Refer to the user manual provided with your external device for contact closure signal information If necessary prime the device and make the injection as described in the user manual provided with the external device Mariner Dual 10 Port Switching Valve Assembly User Guide 3 5 Chapter 3 Connecting the Valve Assembly 3 6 Applied Biosystems 4 Preparing the Valves This chapter contains the following sections 4 1 Making Fluidic Connections 4 2 4 2 Testing the Valves 4 4 Mariner Dual 10 Port Switching Valve Assembly User Guide 4 1 Chapter 4 Preparing the Valves 4 1 Making Fluidic Connections Plumbing diagrams Connecting tubing remove knurled ring to tighten fitting with a wrench 4 2 Applied Biosystems Appendix B Plumbing for Common Applications provides sample plumbing diagrams for the dual 10 port switching valve assembly For additional information on possible configurations refer to the Solutions Book available on Rheodyne s web site www rheodyne com Use Rheodyne RheFlex fittings to connect tubing to the 10 port switching valves WARNING CHEMICAL HAZARD If you run 100 IPA 100 acet2. NOTE When Data Acquisition is disabled the event executes when called but no spectrum is acquired during the event The data file does not include a data point for this event period 5 Select the Contact Out check box then click Set The ContactOut Settings for Event 2 dialog box appears Figure A 4 6 Inthe ContactOut settings for Event 2 dialog box select the options shown in Figure A 4 ContactOut Settings for Event 2 Ea Open Close Ignore ContactOut 1 oi C C ContactOut 2 C O Cc tH g f Cc z it HA D F O ContactOut 5 C C c ContactOut 6 C CN Cancel Figure A 4 ContactOut Settings for Event 2 Dialog Box A 6 Applied Biosystems Creating the Example MS Method 7 Click OK Valve 1 is set to Position A and Valve 2 is set to Position B 8 Select Repeat to trigger the contact every time Event 2 occurs Branching for To send the MS Method to Event 1 at the end of Event 2 Event 2 4 Select Always Go To 2 Inthe Segment drop down list select 1 3 Inthe Event drop down list Select 1 Saving the To save the MS Method for future use MS Method 41 Click Save then name the MS Method msmethod for contact switching 2 Click Close Mariner Dual 10 Port Switching Valve Assembly User Guide A 7 Appendix A Example MS Method A 3 Running the Example MS Method A 8 Applied Biosystems Before running the MS Method read the Mariner Workstation
3. WARNING ELECTRICAL SHOCK HAZARD Severe electrical shock which could cause physical injury or death can result from working on an instrument when the high voltage power supply is operating Also failure to power down the system can result in damage to the instrument electronics To avoid electrical shock disconnect the power supply to the instrument unplug the power cord and wait at least 1 minute before working on the instrument After powering up internal elements of the system can take up to 1 5 hours to equilibrate To remove the panels 1 Shut down the Mariner system as described in the Mariner Workstation User Guide Section 8 3 General Maintenance 2 Remove the two screws holding the Mariner front panel in place Figure 2 1 PB100821 Figure 2 1 Removing the Front Panel Optional Integrated Syringe Pump Shown 3 Gently slide the panel to the right and forward a few inches Before You Begin 4 Unplug the fan cable from the internal connector board If you have the optional integrated syringe pump installed unplug the two additional syringe pump cables and the loop injector cable 5 Set aside the front panel Remove inner 6 Loosen the two screws on the left of the inner rear panel rear panel and remove the two screws on the right Set aside the two removed screws Figure 2 2 7 Slide the inner rear panel to the right and lift it out 8 Discard or store the panel Th
4. Mariner Dual 10 Port Switching Valve Assembly User Guide A AR Be elie Copyright 2001 Applied Biosystems All rights reserved For Research Use Only Not for use in diagnostic procedures Information in this document is subject to change without notice Applied Biosystems assumes no responsibility for any errors that may appear in this document This document is believed to be complete and accurate at the time of publication In no event shall Applied Biosystems be liable for incidental special multiple or consequential damages in connection with or arising from the use of this document Applied Biosystems Delayed Extraction SymBiot and the fractal icon are registered trademarks of Applera Corporation or its subsidiaries in the U S and certain other countries AB Design ABI Applera BioCAD Biospectrometry Data Explorer INTEGRAL Mariner SPRINT and VISION are trademarks of Applera Corporation or its subsidiaries in the U S and certain other countries Acrobat and Adobe are registered trademarks of Adobe Systems Incorporated Teflon is a registered trademark of E I Du Pont de Nemours and Co Microsoft MS MS DOS Visual Basic Windows and Windows NT are registered trademarks of Microsoft Corporation RheFlex is a registered trademark and Rheodyne is a trademark of Rheodyne L P PEEK is a trademark of the Victrex Corporation All other trademarks are the sole property of their respective owners Print
5. on page 6 5 Two column clips each compatible with columns up to 24 mm in diameter and 30 cm in length Internal pneumatic control The dual 10 port switching valve assembly requires compressed dry gas air or nitrogen to operate Gas from the Mariner Workstation Gas 4 connector supplies a small gas cylinder inside the valve assembly When you switch the valve gas is drawn from the gas cylinder rather than directly from the gas line This method stabilizes pressure and prevents fluctuation in the curtain gas flow rate 2 Installing the Valve Assembly This chapter contains the following sections PS De sorron daiatanbiaiistinea 2 2 2 2 When ta siall cerra 2 2 2 3 Before YOu Begin sscan 2 3 2 4 Installing the Valve Assembly 2 6 Mariner Dual 10 Port Switching Valve Assembly User Guide 2 1 Chapter 2 Installing the Valve Assembly 2 1 Overview The Mariner dual 10 port switching valve assembly is usually installed by Applied Biosystems Use the information in this during troubleshooting PA 2 2 When to Install section if you need to remove and replace the assembly The installation procedure for the 10 port switching valve assembly differs depending on the serial number of your instrument and whether you purchased the valve with the instrument or later as an accessory The following table shows the installation procedure for each situation Instrument Serial When Valve Assembl
6. supply line is unobstructed 5 Slide the assembly to the left and into place aligning the left screw holes under the left screws 6 Reinstall the two right screws and tighten the two left screws Connect power 7 Draw the ribbon power cable across the front of the cable pressurized gas cylinder A red line on the cable indicates its top edge Connect the cable to J10 on the internal connector board Figure 2 4 Pin 1 is at the top of the connector NOTE Avoid twisting the cable Keeping the cable flat ensures the correct pin alignment is maintained Z Internal n connector board H Pin 1 at the top of the connector J10 de switching Red line lve power indicating va cable Pin 1 PB100809 Figure 2 4 Connecting the Switching Valve Power Cable Mariner Dual 10 Port Switching Valve Assembly User Guide 2 7 Chapter 2 Installing the Valve Assembly Replace front panel 2 8 Applied Biosystems 8 Position the front panel at the front of the opening 9 Connect the fan power cable from the front panel to the J6 connector Figure 2 5 on the internal connector board If you have the optional integrated syringe pump on the front panel connect the three additional cables The table below summarizes the connections Cable Connector Fan J6 Syringe pump power Optional J7 Syringe pump signal Optional Syr Syri
7. Contact Out Event Count 2 2 Event Setting r Actions Branching IV Instrument Setting Import BIC File Export BIC File View Edit Settings Always Go To Seg 1 Event j FN Data Acquisition C Conditional deceit Repeat C Only Once ee L M Contact Out When True Event Tag Sega 1 7 Event p When False Types Seg 7 Event i Z Save Save As Print Close Set contact out for selected event Figure 5 1 MS Method Editor Dialog Box 5 2 Applied Biosystems MS Method Control NOTE See the Mariner Workstation User Guide Chapter 7 Using MS Method for information on creating an MS Method An example MS Method is provided in Appendix A Example MS Method of this document 2 Setthe Contact Out for the Segment Setting and Event Setting as appropriate Refer to the following table for contact out settings MS Method VAN Pasintan Contact Out Settings Valve 1 Position A Contact out 1 open Valve 1 Position B Contact out 1 close Valve 2 Position A Contact out 2 open Valve 2 Position B Contact out 2 close Mariner Dual 10 Port Switching Valve Assembly User Guide 5 3 Chapter 5 Controlling the 10 Port Switching Valves 5 3 External Device Control Connect the device as described in External Device Control on page 3 5 of this document Refer to the user manual provided with the external device for information on contact cl
8. Fittings preventing leaks at high pressure 4 3 Flow paths valve positions A and B B 4 Front panel removing 2 4 replacing 2 8 Mariner Dual 10 Port Switching Valve Assembly User Guide Index 1 G Gas supply line connecting 2 6 H Help See Applied Biosystems technical support l Injection valve installing 2 4 Installing before you begin 2 3 integrated injection valve 2 4 integrated syringe pump 2 4 modem 2 1 positioning valve assembly 2 7 removing front panel 2 4 removing inner rear panel 2 5 removing original panels 2 3 replacing front panel 2 8 software 2 2 syringe pump 2 4 valve assembly 2 2 2 6 when to install 2 2 Instrument safety ix L Leaks checking 4 4 preventing 4 3 LED contact mode 3 3 control mode 3 3 pulse mode 3 3 Index 2 Applied Biosystems M Maintaining front panel fan 6 5 valves 6 2 Mariner MS Method control 3 4 Modem connecting 2 1 testing 5 1 MS Method conditions that trigger an event A 6 A 7 contact closure A 5 A 6 controlling valves 3 4 5 2 creating A 3 duration A 8 event branching setting A 6 A 7 events setting A 4 A 5 A 6 example A 1 running A 8 running and acquiring data A 8 saving A 7 segments setting A 3 starting A 8 status A 9 stopping A 9 testing A 8 O Overview 10 port switching valve assembly 1 2 connectors 3 2 remote troubleshooting 1 2 P Parts of the 10 port switching valve assembly 1 3 spare C 6 Plumbing for calibrant i
9. User Guide Section 7 4 Running an MS Method Check the following parameters in the Mariner Instrument Control Panel 1 2 Verify Instrument State set to On From the View menu on the Instrument Control Panel select Data Storage then enter or select e Root Directory e Directory File Name e Standby at End of Acquisition e Start On conditions including Start Delay time e Comment information For information see the Mariner Workstation User Guide Section 4 8 8 Data Storage NOTE Stop On conditions and Repeat Acquisition are ignored when you run an MS Method Acquisition duration is determined by MS Method Total Run Time From the Method menu select one of the following e Run Starts the MS Method without acquiring data This option is useful to test the timing and event actions in an MS Method before acquiring data e Run and Acquire Starts the MS Method and acquires data In the displayed dialog box select msmethod for contact switching msm then click Open The method runs Data is acquired if you selected the Run and Acquire command Displaying MS Method Status A 4 Displaying MS Method Status To display MS Method status select Acquisition Status from the View menu MS Method Status is displayed at the bottom of the Acquisition status page Figure A 5 The name of the data file acquired by the MS Method is displayed in the Acquisition Status section of the page Total Time Remain
10. been detected Please check valves then select OK Figure 6 2 Switching Valve Fault Error Message Mariner Dual 10 Port Switching Valve Assembly User Guide 6 3 Chapter 6 Maintenance and Troubleshooting Clearing faults ifthe yellow Fault LED is illuminated or you receive a switching valve fault error message 1 Check that all connections to the valve assembly are secure 2 Check that the gas flow is at the appropriate pressure 3 Switch the valve state with a contact closure signal from either the Mariner MS Method software or the external device For more information see Chapter 5 Controlling the 10 Port Switching Valves 4 Click OK to clear the error message and continue acquisition If you are unable to resolve the problem call Applied Biosystems Technical Support 6 4 Applied Biosystems Maintaining the Front Panel Fan 6 3 Maintaining the Front Panel Fan The front panel fan Figure 6 3 contains a replaceable filter PB100803 Figure 6 3 Front Panel Fan WARNING CHEMICAL WASTE HAZARD Wear appropriate eyewear clothing and gloves when handling a dirty filter Dispose of the dirty filter in accordance with all applicable local state and federal health and environmental regulations Mariner Dual 10 Port Switching Valve Assembly User Guide 6 5 Chapter 6 Maintenance and Troubleshooting To replace the fan filter 1 Remove the dirty filt
11. the Mariner MS Method feature or an external device 3 3 1 Mariner MS Method Control The MS Method feature in the Mariner Instrument Control Panel allows you to trigger valve actions based on time or conditions using the Mariner contact outputs See Appendix A Example MS Method To connect the Mariner MS Method contacts 1 Connect the Mariner Workstation 8 pin output connector on the rear of the right side panel to the J6 external connector on the valve assembly Figure 3 3 connector PB100803 Figure 3 3 Dual 10 Port Switching Valve Assembly and Mariner Connectors 3 4 Applied Biosystems 3 3 2 Connecting the Valve Controls 2 Connect the pins on the Mariner 8 pin connector to the J6 external connector pins as specified in the following table Valve Number and 8 pin J6 Corresponding Position Connector Connector MS Method Setting Valve 1 Position A Pin 1 Pint Contact out 1 open Valve 1 Position B Pin 2 Pin 2 Contact out 1 close Valve 2 Position A Pin3 Pin 3 Contact out 2 open Valve 2 Position B Pin 4 Pin 4 Contact out 2 close External Device Control To control the valves using an external device such as an LC system connect your external device to the J6 connector Pins 1 and 2 control Valve 1 and Pins 3 and 4 control Valve 2 The following table indicates the valve control assignments Valve Number and J6 Connector Contact Signal
12. MANDATION La lampe peut tre endommag e N y touchez pas Un avertissement fournit une information indispensable la s curit de l operateur Par exemple AVERTISSEMENT RISQUE CHIMIQUE I convient de se familiariser avec la MSDS feuille de donn es concernant la s curit des mat riaux avant d utiliser des r actifs ou des solvants Safety and EMC Compliance Information Safety symbols The following symbols may be displayed on the system Electrical symbols These symbols may also appear next to associated warnings in this document The following table describes electrical symbols that may be displayed on your instrument Whenever such symbols appear on instruments observe appropriate safety procedures Indicates the On position of the main power switch Indicates the Off position of the main power switch Indicates the On Off position of a push push main power switch Indicates that a terminal may be connected to the signal ground reference of another instrument This is not a protected ground terminal Indicates a protective grounding terminal that must be connected to earth ground before any other electrical connections are made to the instrument Indicates a terminal that can receive or deliver alternating current or voltage Indicates a terminal that can receive or supply alternating or direct current or voltage Appears next to the values of the fuses re
13. Mariner Regenerates B Figure B 6 Regenerates Elutes to the Mariner The columns regenerate with gradient buffer at the starting composition Mariner Dual 10 Port Switching Valve Assembly User Guide B 9 Appendix B Plumbing for Common Applications Eluent pump Autosampler Regeneration p PUMP pE FT M RE 1 Column 2 lt To waste Column 1 Eluent flow Regeneration PB100814A flow To Mariner Figure B 5 Position A Column 2 is Regenerated Eluent pump Regeneration Autosampler pump FES 5 y YY Y Column 2 Vy p P L TLU es I gt L _ es OOA l To waste Column 1 W eee Eluent flow l PB100814B Pois RS Regeneration o Mariner flow Figure B 6 Position B Column 1 is Regenerated B 10 Applied Biosystems C Warranty Service Information This appendix contains the following sections C 1 Limited Product Warranty 2 C2 Damages Claims Returns C 5 C3 Spare Pans ocni C 6 Applied Biosystems supplies or recommends certain configurations of computer hardware software and peripherals for use with its instrumentation Applied Biosystems reserves the right to decline support for or impose charges for supporting nonstandard computer configurations or components that have not been supplied or recommended by Applied Biosystems Applied Biosystems also reserves the right to require that computer hardware and s
14. PB100881 Figure 3 1 Dual 10 Port Switching Valve Assembly External Connectors Valves 1 and 2 are independently operated with either contact closure signals or pulse signals You cannot control the valves using transistor transistor logic TTL The signals that control the two 10 port valves use Pins 1 through 4 top four of the external J6 connector Pins 5 and 6 are for future use The two positions for each valve are e Position A Contact open default position e Position B Contact closed Four green LEDs on the front panel of the 10 port switching valve assembly indicate the position of the valves The switching time from Position A to B is approximately 1 second 3 2 Applied Biosystems Selecting Control Mode 3 2 Selecting Control Mode The 10 Port switching valves can operate in two modes e Contact mode e Pulse mode The control mode LED located on the valve panel between Valve 1 and Valve 2 Figure 3 2 indicates the current control mode of the 10 Port Switching Valves e On Contact Mode Off Pulse Mode To switch between the two control modes push the control mode selection button Figure 3 2 Valve 1 Control mode selection button Control mode LED recessed LT Figure 3 2 Selecting Control Mode Mariner Dual 10 Port Switching Valve Assembly User Guide 3 3 Chapter 3 Connecting the Valve Assembly 3 3 Connecting the Valve Controls You can control the valves using
15. Position A and or B as appropriate The switching time from Position A to Position B is approximately 1 second Mariner Dual 10 Port Switching Valve Assembly User Guide B 5 Appendix B Plumbing for Common Applications B 3 1 Calibrant Introduction Overview The calibrant introduction configuration allows you to inject calibrant before running your sample In this configuration the syringe pump pushes calibrant into the sample loop and then flow from an external device pushes the calibrant from the sample loop into the Mariner workstation Examples of external devices are an LC system another valve or a pump Flow Table B 1 describes the delivery sequence for the calibrant Description introduction configuration Table B 1 Calibrant Introduction Flow Paths Valve Position Syringe Pump External Source A A continuous flow of calibrant is Flow from the external Figure B 2 delivered from the syringe pump device is delivered to the through the sample loop and to waste Mariner This fills the sample loop with calibrant B Syringe pump is offline Flow from the Flow from the external Figure B 3 syringe pump goes directly to waste device is delivered into the sample loop pushing calibrant into the Mariner B 6 Applied Biosystems Common Applications Flow from external device To waste PB100815A To Mariner Figure B 2 Position A Delivering Calib
16. Valves Describes how to control the valves from Mariner MS Method software Chapter 6 Maintenance and Troubleshooting Provides information on maintaining the valves and front panel fan and describes the procedure for troubleshooting the valves Mariner Dual 10 Port Switching Valve Assembly User Guide v How to Use This Guide Chapter Content Appendix Example Provides a sample MS Method for controlling the valves MS Method Appendix B Plumbing Describes how to plumb the valves and provides flow for Common diagrams for common LCMS applications Applications Appendix C Contains warranty service and return information Warranty Service Information Conventions This guide uses the following conventions e Bold text indicates user action For example Type 0 and press Enter for the remaining fields e Italic text denotes new or important words and is also used for emphasis For example Before analyzing always prepare fresh matrix Related These related documents are shipped with your system documentation Mariner Workstation User Guide Describes Mariner software e Data Explorer User Guide Describes Data Explorer software e Mariner Workstation Getting Started Guide Describes basic information designed to help you quickly learn to use the Mariner workstation e Spray Chamber Supplements Describe the procedures for installing
17. and using optional spray chambers vi Applied Biosystems How to Use This Guide __ Printer documentation depends on the printer you purchase Describes how to set up and service your printer Microsoft Windows NT User s Guide and related documents Provides detailed information about the Microsoft Windows NT user interface Portable document format PDF versions of the Applied Biosystems documents listed above and this supplement are also available on the Mariner software installation CD Reference This reference document is shipped with your system documentation Varian Rotary Vane Pumps Instruction Manual Provides maintenance procedures for the external mechanical pump Send us your Applied Biosystems welcomes your comments and comments suggestions for improving its manuals You can send your comments in two ways e Use the Technical Publications Customer Survey at www appliedbiosystems com PerSeptive techsup survey htm Send e mail to techpubs appliedbiosystems com Mariner Dual 10 Port Switching Valve Assembly User Guide vii How to Use This Guide viii Applied Biosystems Safety and EMC Compliance Information Safety and EMC Compliance Information This section includes e Instrument safety e Safety and EMC standards Instrument Safety This section includes e Notes Hints Cautions and Warnings e Safety symbols e Before operating this instrument e Material Safety Da
18. and workmanship for a period of ninety 90 days Applied Biosystems will repair or replace at its discretion defective accessories during this warranty period After this warranty period Applied Biosystems will pass on to the buyer to the extent that it is permitted to do so the warranty of the original manufacturer for such accessories With the exception of consumable and maintenance items replaceable products or components used on the instrument are themselves warranted to be free of defects in materials and workmanship for ninety 90 days Applied Biosystems warrants that chemicals and other consumable products will be free of defects in materials and workmanship when received by the buyer but not thereafter unless otherwise specified in documentation accompanying the product Warranty period effective date Warranty exceptions Limited Product Warranty Applied Biosystems warrants that for a period of ninety 90 days from the date of installation the software designated for use with the product will perform substantially in accordance with the function and features described in its accompanying documentation when properly installed on the product Applied Biosystems does not warrant that the operation of the instrument or software will be uninterrupted or error free Applied Biosystems will provide any software corrections or bug fixes if and when they become available for a period of ninety 90 days after install
19. ation Any applicable warranty period under these sections will begin on the date of installation for hardware and software installed by Applied Biosystems personnel unless that date has been delayed at the buyer s request In that case and for all hardware and software installed by the buyer and for all other products the applicable warranty period begins the date the component is received by the buyer The above warranties shall not apply to defects resulting from misuse neglect or accident including without limitation operation with incompatible solvents or samples in the system operation outside of the environmental or use specification instructions for the product or accessories performance of improper or inadequate maintenance by the user installation of software or interfacing not supplied by Applied Biosystems and modification or repair of the product or the software not authorized by Applied Biosystems The foregoing provisions set forth Applied Biosystems sole and exclusive representations warranties and obligations with respect to its products and Applied Biosystems makes no other warranty of any kind whatsoever expressed or implied including without limitation warranties of merchantability and fitness for a particular purpose whether arising from a statute or otherwise in law or from a course of dealing or usage of trade all of which are expressly disclaimed Such limited warranty is given only to buyer or any
20. e by any agent employee or representative of Applied Biosystems will not be binding on Applied Biosystems This warranty is limited to the buyer of the product from Applied Biosystems and is not transferable Damages Claims Returns C 2 Damages Claims Returns Damages Unpack shipments promptly after receipt to check for any concealed damage If you discover damage stop unpacking Contact the shipping carrier and request inspection by a local agent Secure a written report of the findings to support any claim Do not return damaged goods to Applied Biosystems without first securing an inspection report and contacting Applied Biosystems Technical Support for a Return Authorization RA number Claims After a damage inspection report is secured Applied Biosystems will supply the replacements and process claims that are initiated by either party Returns Do not return any material without prior notification and authorization If for any reason it becomes necessary to return material to Applied Biosystems contact Applied Biosystems Technical Support or your nearest Applied Biosystems subsidiary or distributor for e Return Authorization RA number e Forwarding address e Decontamination instructions e Packing and shipping instructions Place the RA number in a prominent location on the outside of the shipping container and return the material to the address designated by the Applied Biosystems representative Marine
21. e data acquisition NOTE When Data Acquisition is disabled the event executes when reached but no data point is acquired during the event The data file does not include a data point for this event period 5 Select the Contact Out check box then click Set The ContactOut Settings for Event 1 dialog box appears Eigure A 3 In the ContactOut Settings for Event 1 dialog box select the options shown in Figure A 3 ContactOut Settings for Event 1 x Open Close Ignore ContactOut 1 C o c ContactOut 2 ON C 2 Intactiut He fi CG E Intactuut HA a 2 Cc ContactOut 5 Cc C o ContactOut 6 e oi Cancel Figure A 3 ContactOut Settings for Event 1 Dialog Box Click OK Valve 1 is set to Position B and Valve 2 is set to Position A Select Repeat to trigger the contact every time Event 1 occurs Mariner Dual 10 Port Switching Valve Assembly User Guide A 5 Appendix A Example MS Method Branching for To send the MS Method to Event 2 at the end of Event 1 Event 1 4 Select Always Go To 2 Inthe Segment drop down list select 1 3 In the Event drop down list select 2 Event 2 actions To specify Event 2 actions 1 Select Event 2 in the event bar 2 Select the Instrument Setting check box then click Import BIC File 3 Select an appropriate BIC file 4 Select the Data Acquisition check box to enable data acquisition or deselect to disable data acquisition
22. e dual 10 port switching valve assembly replaces the panel PB100805 Figure 2 2 Removing the Inner Rear Panel Mariner Dual 10 Port Switching Valve Assembly User Guide 2 5 Chapter 2 Installing the Valve Assembly 2 4 Installing the Valve Assembly To install the dual 10 port switching valve assembly Connect gas _ 1 Check that gas pressure for the gas supply to the supply line Gas 4 connector is 80 to 100 psi 550 to 690 kPa WARNING EXPLOSION HAZARD Pressurized gas cylinders are potentially explosive Always cap the gas cylinder when it is not in use and attach it firmly to the wall or gas cylinder cart with approved brackets chains or clamps 2 Within the inner rear panel opening locate the gas supply line white Teflon tubing labeled G4 Gently pull the unattached end of the gas supply line through the rear panel opening 3 Connect the gas supply line to the fitting on the back of the dual 10 port switching valve assembly Figure 2 3 Gently insert the tubing into the fitting until firmly seated NOTE When the tubing is firmly seated you can pull on it without dislodging it from the fitting Figure 2 3 Connecting the Gas Supply Line 2 6 Applied Biosystems Installing the Valve Assembly Position valve 4 Position the rear panel of the dual 10 port switching valve panel assembly in the rear panel opening Check that the gas
23. ed in the USA 01 2001 Part Number V900206 Rev A Table of Contents Table of Contents How to Use This Guide 0 0 cestesteseeesteneeneenen vii Safety and EMC Compliance Information ix Chapter 1 The Dual 10 Port Switching Valve Assembly 1 1 COVOIVIOW Essentiel Mat ns nt et te ta 1 2 1 2 Parts of the AsS mbDIV canere dinn ae An ne tn an meer ce eed 1 3 Chapter 2 Installing the Valve Assembly 2 1 OVET EW sen RS MR ERA cee dh et AT ARR tete ee 2 2 2 2 Whento Install 232 22 agir a a See 2 2 2 3 Before You Begin 2 3 2 3 1 Safety Information ccceceeeeeeeeeeeeeeeeeeeeeaeeaeeaaeteeeeeeeeeeeeeees 2 3 2 3 2 Removing Original Panels cccceceeeeeeeeeeaeaeeeeeeeteeeeeeeeeees 2 3 2 4 Installing the Valve Assembly 000 eect eeeeeeeeaaaeeeeeeeeaaaeeeeeeeeaaaaeeeees 2 6 Chapter 3 Connecting the Valve Assembly 3 1 OVOIVIOW RER D Deleon aa a a a 3 2 3 2 Selecting Control Mode cccceeceeeeeee eee ae eeeeeeeeeeeeeeeeeeaaeaaeaeeeeeeeeeeees 3 3 3 3 Connecting the Valve Controis 3 4 3 3 1 Mariner MS Method Control ccccccccccsscceeeeceseeeeeeenseeaaees 3 4 3 3 2 External Device Control ccccccsccceeececeseceeeeeeceaeseeseeeesaaes 3 5 Chapter 4 Preparing the Valves 4 1 Making Fluidic Connections 4 2 4 2 Testing the Valves ss dus late heat la dass tthe aenagbennes 4 4 Dual 10 Port Switching Valve Assembly User Guide
24. er by pulling the release bar Figure 6 4 The dirty filter drops down 2 Slide a new filter part number V106165 in and up until it locks in place ae mn Es Las el _ aes a Ta eee _ mn Release bar PB100810 Figure 6 4 Replacing the Fan Filter 6 6 Applied Biosystems A Example MS Method This appendix contains the following sections Ai Example MS Method A 2 fa D O S m x 3 o ep p fs j P co B Co JD em 2 F m x 3 o wn fe 2 P Ex E O1 e vV U o o O jo 5 gt 9 jo gt a le gt 1 E em o a P Lo ko Mariner Dual 10 Port Switching Valve Assembly User Guide A 1 Appendix A Example MS Method A 1 Example MS Method Before you begin Read the Mariner Workstation User Guide Chapter 7 Using MS Method before proceeding Overview The example MS Method is designed to cycle through all the valve positions The stages of the cycle are At the start of the segment e Valve 1 is set to Position A Valve 2 is set to Position A In Event 1 e Valve 1 is switched to Position B e Valve 2 remains in Position A In Event 2 e Valve 1 is switched to Position A e Valve 2 is switched to Position B At the end of the segment e Valve 1 remains in Position A e Valve 2 is switched to Position A The follo
25. ess or death Always determine what chemicals have been used in the instrument before changing reagents or instrument components Read and understand the Material Safety Data Sheets MSDSs provided by the chemical manufacturer before you store handle or work with any chemicals or hazardous materials Minimize contact with and inhalation of chemicals Wear appropriate personal protective equipment when handling chemicals e g safety glasses gloves or clothing Consult the listing in the MSDS Do not leave chemical containers open Check regularly for chemical leaks or spills If a leak or spill occurs follow the manufacturer s clean up procedures as recommended on the MSDS Comply with all applicable laws and regulations related to chemical storage handling and disposal B 2 Applied Biosystems Plumbing the Valves WARNING CHEMICAL WASTE HAZARD Wastes produced by Applied Biosystems instruments are potentially hazardous and can cause injury illness or death Read and understand the material safety data sheets MSDSs provided by the manufacturers of the chemicals in the waste container before you store handle or dispose of chemical waste Handle chemical wastes in a fume hood Minimize contact with and inhalation of chemical waste Wear appropriate personal protective equipment when handling chemicals e g safety glasses gloves or clothing Always mix and prepare hazardous materials beneath an o
26. fety This instrument meets European requirements for safety EMC Directive 73 23 EEC This instrument has been tested to and complies with standard EN61010 1 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use EMC This instrument meets European requirements for emission and immunity EMC Directive 98 336 EEC This product has been evaluated to the EN55011 1992 Group 1 Class B Radiated Emissions and EN50082 1 1992 Generic Immunity 1 The Dual 10 Port Switching Valve Assembly This chapter contains the following sections LU GIO naines nn Anne dents nb t 1 2 1 2 Parts of the Assembly 1 3 Mariner Dual 10 Port Switching Valve Assembly User Guide 1 1 Chapter 1 The Dual 10 Port Switching Valve Assembly 1 1 Overview The dual 10 port switching valve assembly is designed for use with the Mariner Workstation The assembly consists of two pneumatically controlled 10 port two position switching valves two column clips and seven external connectors Figure 1 1 10 port switching valves External connectors Column clips PB100803 Figure 1 1 Mariner Dual 10 Port Switching Valve Assembly With the addition of the dual 10 port switching valve assembly the Mariner becomes a more flexible LCMS workstation For example with the switching valve you can perform e Sample injection e Calibrant introduction e Salt diversi
27. iarize yourself with the MSDSs before using reagents or solvents AVERTISSEMENT RISQUE CHIMIQUE II convient de se familiariser avec la MSDS feuille de donn es concernant la s curit des mat riaux avant d utiliser des r actifs ou des solvants Mariner Dual 10 Port Switching Valve Assembly User Guide XV Safety and EMC Compliance Information General Warnings gt gt p p xvi Applied Biosystems WARNING FIRE HAZARD Using a fuse of the wrong type or rating can cause a fire Replace fuses with those of the same type and rating AVERTISSEMENT DANGER D INCENDIE L usage d un fusible de type ou de valeur nominale diff rents risque de provoquer un incendie Il convient donc de remplacer les fusibles usag s par des fusibles du m me type et de la m me valeur nominale WARNING ELECTRICAL SHOCK HAZARD Severe electrical shock can result from operating the instrument without its front or side panels in place Do not remove instrument front or side panels High voltage contacts are exposed when front or side panels are removed AVERTISSEMENT RISQUE DE D CHARGE LECTRIQUE Des d charges lectriques s rieuses peuvent r sulter du fonctionnement de l appareil lorsque le panneau avant et les panneaux lat raux sont retir s Ne pas retirer le panneau avant ou les panneaux lat raux Des contacts haute tension sont expos s lorsque les panneaux sont reti
28. igure A 1 MS Method Editor Dialog Box Method setting To specify the Method Setting Figure A 1 1 Select Reset All Contact Out at End to set contacts to the initial state of Open at the end of the MS Method 2 Set the Total Number of Segments to 1 Mariner Dual 10 Port Switching Valve Assembly User Guide A 3 Appendix A Example MS Method for 10 minutes Figure A 2 Segment setting To specify the segment setting 1 Inthe Duration field enter 00 10 00 to run the segment 2 Select the Contact Out check box then click Set The ContactOut settings for Segment 1 dialog box appears 3 Inthe ContactOut settings for Segment 1 dialog box select the options shown in Figure A 2 ContactOut 1 ContactOut 2 ContactOut 5 ContactOut 6 ContactOut Settings for Segment 1 x Open Close Ignore O 3 e eek e i L o eo oe e ON Cancel Figure A 2 ContactOut Segment Settings Dialog Box 4 Click OK Valve 1 and Valve 2 are set to Position A 5 If you want to create two events for the segment select 2 in the Event Count selection box A 4 Applied Biosystems Event 1 actions 1 Creating the Example MS Method Select Event 1 in the event bar Figure A 1 on page A 3 Select the Instrument Setting check box then click Import BIC File Select an appropriate BIC file Select the Data Acquisition check box to enable data acquisition or deselect to disabl
29. iii Table of Contents Chapter 5 Controlling the 10 Port Switching Valves 5 1 OVERVIEW Eine tock RE M nt mA Re en tn dite 5 2 MS Method Control iiiiiiiiiieeiieiieceereeeresceeseeses 5 3 External Device Control iii Chapter 6 Maintenance and Troubleshooting 6 1 Maintaining the Valves 6 2 Troubleshooting the Valves 6 3 Maintaining the Front Panel Fan Appendix A Example MS Method A 1 Example MS Method ccccececce cece ee aaeeeeeeeeeeeeeeeeeeeeseeaeaaeaneeeeeeeeeeeenees A 2 Creating the Example MS Method ccceceeeeee eee eeneeeeeeeeeeeeeeeeeeeeees A8 Running the Example MS Method ccccceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeees A 4 Displaying MS Method Status 00 ccceceeeeeeeeeeeeeeaeeaeeeeeeeeeeeeeeeeeeeeeees A 5 Stopping an MS Method cee ecececceceeeeeeeeeeeeeeeeeeeeeaeeaaaaeeeeeeeeeeeeeeeeees Appendix B Plumbing for Common Applications B 1 Plumbing the Valves sus B 2 Valve Flow Paths nonna ra ia Beebe dec Rene SE B 3 Common Applications B 3 1 Calibrant Introduction cecccceeeeeeeee eee aeeeeeeeeeeeeeeeeeeeaeeanaaaea B 3 2 Sample Cleanup cceeeeeeeeeeeeeeeeeeeeeeaeeaeaaeeteeceeeeeeeeeeeaeeaaa B 3 3 Column SWitChing sr russie art ccovendeenctedebenteenevess Appendix C Warranty Service Information C 1 Limited Product Warrant
30. ing 00 00 00 Seg Time Remaining 00 00 00 DSSS SS Sed Acquistion Stats Acauisition OFF Elapsed Duration o0 00 00 Data File MS Method Status z Run Status OFF Figure A 5 MS Method Status A 5 Stopping an MS Method To stop an MS Method select Stop from the Method menu Mariner Dual 10 Port Switching Valve Assembly User Guide A 9 Appendix A Example MS Method A 10 Applied Biosystems B Plumbing for Common Applications This appendix contains the following sections B 1 Plumbing the Valves assssnrressrrnrrnnnan B 2 B2 Valve Flow Pathe ocrni B 4 B 3 Common Applications ee B 5 B 3 1 Calibrant Introduction B 6 B 3 2 Sample Cleanup see B 8 B33 Column Switching usica B 9 Mariner Dual 10 Port Switching Valve Assembly User Guide B 1 Appendix B Plumbing for Common Applications B 1 Plumbing the Valves Overview Plumb the 10 port switching valves with 1 16 inch ID tubing Plumb for Position A and or B as appropriate The switching time from Position A to Position B is approximately 1 second Before you Before you begin read Chapter 4 Preparing the Valves and begin Chapter 5 Controlling the 10 Port Switching Valves In addition read the safety information in Safety and EMC Compliance Information on page ix WARNING CHEMICAL HAZARD Some of the chemicals used with Applied Biosystems instruments are potentially hazardous and can cause injury illn
31. ls on system xi Warranty damages claims returns C 5 description C 2 exceptions C 3 for computers with altered configuration C 1 period C 2 Index 4 Applied Biosystems Headquarters 850 Lincoln Centre Drive Foster City CA 94404 USA Phone 1 650 638 5800 Toll Free In North America 1 800 345 5224 Fax 1 650 638 5884 Worldwide Sales and Support Applied Biosystems vast distribution and service network composed of highly trained support and applications personnel reaches into 150 countries on six continents For sales office locations and technical support please call our local office or refer to our web site at www appliedbiosystems com www appliedbiosystems com Applied Biosystems Applera Corporation is committed to providing the world s leading technology and information for life scientists Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses Printed in the USA 01 2001 Part Number V900206 Rev A an Applera business
32. lved with the operation of the the instrument instrument has e Received instruction in general safety practices for laboratories e Received instruction in specific safety practices for the instrument e Read and understood all related MSDSs CAUTION Avoid using this instrument in a manner not specified by Applied Biosystems Although the instrument has been designed to protect the user this protection can be impaired if the instrument is used improperly xiv Applied Biosystems Safety and EMC Compliance Information Material Safety Some of the chemicals that may be used with your system are Data Sheets MSDSs listed as hazardous by their manufacturers Hazards are prominently displayed on the labels of all chemicals In addition MSDSs supplied by the chemical manufacturer provide information about e Physical characteristics e Safety precautions e Health hazards e First aid e Spill clean up e Disposal procedures Chemical manufacturers supply a current MSDS before or with shipments of hazardous chemicals to new customers and with the first shipment of a hazardous chemical after an MSDS update MSDSs provide you with the safety information you need to store handle transport and dispose of the chemicals safely Applied Biosystems strongly recommends that you replace the appropriate MSDS in your files each time you receive a new MSDS packaged with a hazardous chemical WARNING CHEMICAL HAZARD Famil
33. nge pump loop injector Optional J5 Slots for KT panel tabs PB100807 Figure 2 5 Dual 10 Port Switching Valve Assembly Internal Connectors Installing the Valve Assembly 10 Fit the front panel in place by inserting the tabs on the front panel of the integrated syringe into the slots on the frame of the workstation Figure 2 5 on page 2 8 11 Secure the front panel with the two screws on the right side of the workstation Figure 2 1 on page 2 4 Powering up Start up the Mariner system as described in the Mariner Workstation User Guide Section 2 7 Startup and Shutdown Mariner Dual 10 Port Switching Valve Assembly User Guide 2 9 Chapter 2 Installing the Valve Assembly 2 10 Applied Biosystems 3 Connecting the Valve Assembly This chapter contains the following sections 3 1 Overview ee Po a joo Connecting the Valve Controls 3 2 Selecting Control Mode 3 3 3 4 Mariner Dual 10 Port Switching Valve Assembly User Guide 3 1 Chapter 3 Connecting the Valve Assembly 3 1 Overview The dual 10 port switching valve assembly has an external connector J6 which is used to control the valves Figure 3 1 The J6 connector is mounted vertically with Pin 1 at the top Valve 1 Position LEDs lt External connector J6 Valve Controls os Pin 1 at top Position LEDs
34. ntroduction B 6 for column switching B 9 for common applications B 1 for sample cleanup B 8 valve position A and B flow paths B 4 valves 4 2 Positioning valve assembly 2 7 Power cable connecting 2 7 Powering down 2 4 Powering up 2 9 Preparing before you begin installation 2 3 for remote troubleshooting 6 1 Pulse mode control 3 3 LED 3 3 R RA number C 5 Remote troubleshooting 1 2 Return Authorization RA number C 5 Returning damaged items C 5 Running the MS Method A 8 S Safety before operating the instrument xiv electrical symbols xi EMC standards xx instrument ix material safety data sheets xv non electrical symbols xii safety and compliance information ix Sample cleanup plumbing B 8 Sample MS Method A 1 Saving an MS Method A 7 Selecting control mode 3 3 Software when to install 2 2 Spare parts C 6 Start condition acquisition MS Method A 8 Starting MS Method A 8 Status MS Method A 9 Stopping MS Method A 9 Syringe pump integrated installing 2 4 T Technical support 6 2 C 1 Testing modem 5 1 valves 4 4 Troubleshooting MS Method A 8 valves 6 3 Tubing connecting 4 2 V Valve controls connecting 3 4 Valves controlling 5 2 installing 2 2 2 6 plumbing 4 2 plumbing for common applications B 1 position A and B flow paths B 4 lt x lt mo2 Mariner Dual 10 Port Switching Valve Assembly User Guide Index 3 W Warnings description ix general xvi safety information ix symbo
35. oftware be restored to the standard configuration prior to providing service or technical support For systems such as Mariner VISION BioCAD SPRINT or INTEGRAL which have built in computers installing unauthorized hardware or software may void the Warranty or Service Plan Mariner Dual 10 Port Switching Valve Assembly User Guide C 1 Appendix C Warranty Service Information C 1 Limited Product Warranty Limited warranty C 2 Applied Biosystems Applied Biosystems warrants that all standard components of the Mariner Dual 10 Port Switching Valve Assembly will be free of defects in materials and workmanship for a period of ninety 90 days Applied Biosystems will repair or replace at its discretion all defective components during this warranty period After this warranty period repairs and replacement components may be purchased from Applied Biosystems at its published rates Applied Biosystems also provides service agreements for post warranty coverage Applied Biosystems reserves the right to use new repaired or refurbished instruments or components for warranty and post warranty service agreement replacements Repair or replacement of products or components under warranty does not extend the original warranty period Applied Biosystems warrants that all optional accessories supplied with its Mariner Workstations such as peripherals printers and special monitors will be free of defects in materials
36. on e Fraction collection e Column backflushing e Multi dimensional multi column strategies such as Solid phase extraction to RP HPLC Sample enrichment Direct injection of complex matrix Gradient stacking lon exchange to reversed phase separation Using two valves allows you to couple any of the above techniques 1 2 Applied Biosystems Parts of the Assembly 1 2 Parts of the Assembly Figure 1 2 shows the components of the dual 10 port switching valve assembly Gas cylinder Control mode selection Fault LED button recessed Valve 1 Position LEDs Control mode LED Column clips Valve 2 J6 external connector Position LEDs PB100880 Figure 1 2 Mariner Dual 10 Port Switching Valve Assembly These components Figure 1 2 include e Two Rheodyne 10 port two position switching valves mounted at a 45 degree angle for easier plumbing e Six LEDs e One fault LED e Two Valve 1 LEDs Position A and B e One control mode LED e Two Valve 2 LEDs Position A and B Mariner Dual 10 Port Switching Valve Assembly User Guide 1 3 Chapter 1 The Dual 10 Port Switching Valve Assembly 1 4 Applied Biosystems Control mode selection button For information see Selecting Control Mode on page 3 3 External Connectors For information see Connecting the Valve Controls on page 3 4 Venting fan and removable filter not shown For information see Maintaining the Front Panel Fan
37. onitrile or 100 THF at pressures above 1 500 psi 10 340 kPa do not use PEEK tubing Using these solvents at pressures above 1 500 psi may cause the PEEK tubing to burst To connect Rheodyne RheFlex fittings to tubing 1 Slip a compression fitting over the end of the tubing 2 Slip a split ferrule over the end of the tubing with the tapered end of the ferrule pointing away from the compression fitting and into the valve Figure 4 1 Make sure at least 1 16 inch of tubing extends beyond the ferrule If necessary PB100018 Figure 4 1 Connecting Rheodyne Fittings to Tubing Making Fluidic Connections 3 Bottom the tubing in a port then finger tighten the compression fitting 4 For high pressure operation 3 000 to 5 000 psi 20 680 to 34 470 KPal tighten fittings 1 4 turn with a wrench You can remove the knurled ring for easier access to the fitting WARNING PHYSICAL INJURY HAZARD Wear appropriate protective eyewear clothing and gloves when inspecting and adjusting fittings Mariner Dual 10 Port Switching Valve Assembly User Guide 4 3 Chapter 4 Preparing the Valves 4 2 Testing the Valves Checking for leaks Checking backpressure 4 4 Applied Biosystems After plumbing the system check for leaks and excessive backpressure The valves are rated for a maximum pressure of 5 000 psi 34 470 kPa 344 bar Start solvent flow at a low rate and ob
38. osure triggers 5 4 Applied Biosystems 6 Maintenance and Troubleshooting This chapter contains the following sections 6 1 Maintaining the Valves 6 2 6 2 Troubleshooting the Valves eeseeenaa 6 3 6 3 Maintaining the Front Panel Fan 6 5 Mariner Dual 10 Port Switching Valve Assembly User Guide 6 1 Chapter 6 Maintenance and Troubleshooting 6 1 Maintaining the Valves 6 2 Applied Biosystems Each valve has an internal rotor seal that provides a high pressure seal against the face of the valve stator The rotor seal wears with use and needs periodic replacing To maintain optimum operation of the 10 port switching valves contact an Applied Biosystems Technical Representative to change the rotor seal To reach Applied Biosystems Technical Support refer to the information on the back cover of this manual Troubleshooting the Valves 6 2 Troubleshooting the Valves Fault LED A yellow Fault LED is located on the valve panel to the right of Valve 1 Figure 6 1 If this LED is illuminated one of the valves failed to switch properly Fault LED PB100882 Figure 6 1 Fault LED for Valves 1 and 2 Error messages In addition the Mariner control software monitors the state of the dual 10 port switching valves If the switching valves do not function properly acquisition is halted and an error message appears Figure 6 2 Mariner Control Panel x switching valve error has
39. perating fume hood Seal the waste container with the cap provided after disposing of the contents Dispose of the contents of the waste container in accordance with good laboratory practices and local state provincial or national environmental and health regulations Information on sample handling is provided in the Mariner Workstation User Guide Section 3 5 Sample Solvent and LC Column Guidelines For additional plumbing information see the Rheodyne Solutions Book available on the Rheodyne web site www rheodyne com Mariner Dual 10 Port Switching Valve Assembly User Guide B 3 Appendix B Plumbing for Common Applications B 2 Valve Flow Paths Figure B 1 shows all the 10 port valve flow paths for reference only The first drawing shows the flow paths for both Position A and Position B The second and third drawings show the internal connecting passages for Position A and Position B respectively Numbers Ports Internal connections 7 for Position A Internal connections for Position B Both Positions Position A Position B PB100811 Figure B 1 Flow Paths for Valve Positions A and B B 4 Applied Biosystems Common Applications B 3 Common Applications Overview This section includes plumbing diagrams for e Section B 3 1 Calibrant Introduction e Section B 3 2 Sample Cleanup e Section B 3 3 Column Switching Plumb the 10 port switching valves with 1 16 inch ID tubing Plumb for
40. quired by the system WARNING Indicates the presence of high voltage and warns you to proceed with caution Mariner Dual 10 Port Switching Valve Assembly User Guide xi Safety and EMC Compliance Information Non electrical The following table describes non electrical safety alert symbols symbols that may be displayed on your instrument WARNING Alerts you to consult the manual for further information and to proceed with caution WARNING Indicates a hot surface or high temperature hazard Proceed with caution when working around these areas to avoid being burned by hot components WARNING Indicates that a laser is present inside the instrument and warns you to avoid exposure to laser light gt epe xii Applied Biosystems Symboles des alertes de s curit Symboles lectriques Safety and EMC Compliance Information Les symboles suivants peuvent tre affich s sur le syst me Dans ce document ces symboles peuvent aussi appara tre c t des avertissements auxquels ils s associent Le tableau suivant donne la signification de tous les symboles lectriques qui figurent sur les appareils En pr sence de l un de ces symboles il est imp ratif de se conformer aux consignes de s curit appropri es Position MARCHE de l interrupteur d alimentation principal Position ARR T de l interrupteur d alimentation principal Positions MARCHE ARRET de l interrupte
41. r Dual 10 Port Switching Valve Assembly User Guide C 5 Appendix C Warranty Service Information C 3 Spare Parts C 6 Applied Biosystems The following spare parts are available from Applied Biosystems Description Part Number Bottle Assembly V700831 Fan Filter 2 36 in V106165 PC Board V750092 Rheodyne Valve Assembly V700829 Solenoid Valve Bank 4318193 Index A Applied Biosystems technical support 6 2 C 1 B Backpressure checking 4 4 Buffer hazards 2 3 C Calibrant introduction B 6 Checking backpressure 4 4 for leaks 4 4 Column switching B 9 Common applications calibrant introduction B 6 column switching B 9 plumbing B 2 sample cleanup B 8 Computer configuration requirement C 1 technical support for altered configuration C 1 Connecting for external device control 3 5 gas supply line 2 6 modem 2 1 power cable 2 7 tubing 4 2 valve controls 3 4 Contact closure control mode 3 3 control mode LED 3 3 programming MS Method for A 5 A 6 Control mode contact mode 3 3 LED 3 2 3 3 pulse mode 3 3 selecting 3 3 selection button 3 2 3 3 Controlling valves description 5 2 external device 5 4 MS Method 5 2 Conventions Notes Hints Cautions and Warnings ix Creating example MS Method A 3 D Damage reporting C 5 E Electromagnetic compliance xx EMC standards xx Example MS Method A 1 External device controlling the valves 5 4 lt x lt mo2 F
42. r s gt P e e Safety and EMC Compliance Information WARNING PHYSICAL INJURY HAZARD Always wear eye protection when working with solvents or any pressurized liquids AVERTISSEMENT Porter toujours des protections pour les yeux lorsque vous manipulez des solvants ou tout autre liquide sous pression WARNING CHEMICAL HAZARD Before handling any chemicals refer to the Material Safety Data Sheet provided by the manufacturer and observe all relevant precautions AVERTISSEMENT RISQUE CHIMIQUE Avant de manipuler des produits chimiques veuillez consulter la fiche de s curit du mat riel fournie par le fabricant et observer les mesures de pr caution qui s imposent Mariner Dual 10 Port Switching Valve Assembly User Guide xvii Safety and EMC Compliance Information xviii Applied Biosystems WARNING PHYSICAL INJURY HAZARD PEEK tubing is a polymeric material Use caution when working with any polymer tubing under pressure 1 Always wear eye protection when close to pressurized polymer tubing Extinguish all nearby flames if using flammable solvents Do not use PEEK tubing that has been severely stressed or kinked Do not use PEEK tubing with tetrahydrofuran or concentrated nitric or sulfuric acid Be aware that Methylene chloride and dimethyl sulfoxide cause PEEK tubing to swell and greatly reduce the rupture pressure of
43. rant to Sample Loop and Flow to Mariner Syringe pump is now offline Flow from external device Sample loop filled with calibrant PB100815B To Mariner Figure B 3 Position B Delivering Calibrant and Flow to Mariner Mariner Dual 10 Port Switching Valve Assembly User Guide B 7 Appendix B Plumbing for Common Applications B 3 2 Sample Cleanup B 8 Applied Biosystems The sample cleanup configuration allows you to desalt a sample online In sample cleanup configuration Figure B 4 Position A diverts the flow from the column to waste Position B directs the flow from the column to the Mariner Workstation See the Mariner Workstation User Guide Section 3 6 Sample Cleanup for additional cleanup techniques and considerations From column Y I PB100813 To Mariner To waste Position A Position B Figure B 4 Plumbing for Online Desalting Common Applications B 3 3 Column Switching Overview Using the column switching configuration allows you to work with one LC column while the second LC column is being regenerated Figure B 5 and Figure B 6 Flow When you use the column switching configuration one column Description elutes sample as the other column regenerates Table B 2 summarizes the eluting and regenerating cycle Table B 2 Column Switching Flow Paths Valve Position Column 1 Column 2 A Figure B 5 Elutes to the
44. serve all fittings for leaks If no leaks are observed slowly increase the flow rate to your application flow rate If you observe leaks adjust or replace the tubing and tighten the fittings If you are using an LC system check the backpressure of the LC system after starting solvent flow Bypass the LC column and UV detector if applicable Check that the system pressure does not exceed the rating of any component in the system 5 Controlling the 10 Port Switching Valves This chapter contains the following sections it ONONO ce 5 2 MS Method Control sainiis iecccscoinstcanenssanates 5 2 External Device Control 5 4 bk Mariner Dual 10 Port Switching Valve Assembly User Guide 5 1 Chapter 5 Controlling the 10 Port Switching Valves 5 1 Overview You can control the 10 port switching valves in two ways Mariner MS Method software e External device 5 2 MS Method Control To control the 10 port switching valves using Mariner software 1 Create an MS Method in the Mariner software Instrument Control Panel by selecting New MS Method from the Method menu The MS Method Editor is displayed Figure 5 1 Set contact out for selected segment MS Method Editor C Mariner Program Methods contact switching 1 Method Setting Total Run Time HH MM SS 00 10 00 M Reset All Contact Out at end Total Number of Segments 1 Segment Setting Duration HH MM 5 00 10 00 FN
45. ta Sheets MSDSs e General Warnings e General Cautions Notes Hints Notes Hints Cautions and Warnings are used in this Cautions and document as follows Warnings A Note provides important information to the operator For example NOTE If you are prompted to insert the boot diskette into the drive insert the diskette then press any key A Hint provides helpful suggestions not essential to the use of the system For example Hint To avoid complicated file naming use Save First to Pass or Save Best Only mode Mariner Dual 10 Port Switching Valve Assembly User Guide ix Safety and EMC Compliance Information Remarques recommandations et avertissements X Applied Biosystems A Caution provides information to avoid damage to the system or loss of data For example CAUTION Do not touch the lamp This may damage the lamp A Warning provides specific information essential to the safety of the operator For example WARNING CHEMICAL HAZARD Familiarize yourself with the MSDSs before using reagents or solvents Une remarque fournit une information importante a l op rateur Par exemple REMARQUE Si on vous demande d ins rer la disquette de d marrage dans le lecteur ins rez la puis appuyez sur n importe quelle touche Une recommandation fournit une information destin e viter des d t riorations du syst me ou la perte de donn es Par exemple RECOM
46. the tubing Be aware that high solvent flow rates gt 40 ml min may cause a static charge to build up on the surface of the tubing Electrical sparks may result AVERTISSEMENT Le tubage PEEK est un mat riau polym re Prenez des pr cautions lorsque vous travaillez avec un tubage polym re sous pression 1 Porter toujours des protections pour les yeux lorsque vous vous trouvez proximit d un tubage polym re sous pression teindre toutes les flammes avoisinantes si vous utilisez des solvants inflammables Nes pas utiliser un tubage PEEK qui a d j t s rieusement travaill ou pli Safety and EMC Compliance Information 4 Nes pas utiliser un tubage PEEK avec du t trahydrofurane ou des acides nitriques et sulfuriques concentr s 5 Le chlorure de m thyl ne et le sulfoxyde de dim thyle entrainent un gonflement du tubage PEEK et r duisent fortement la r sistance la rupture du tubage 6 Avec des d bits d coulement de solvants lev s gt a 40 ml min une charge statique puet se d velopper sur la surface du tubage Des tincelles lectriques peuvent en r sulter WARNING PHYSICAL INJURY HAZARD Use this product only as specified in this document Using this system in a manner not specified may result in injury or damage to the system AVERTISSEMENT Veuillez suivre avec attention les indications figurant dans ce document lorsque vous
47. third party in the event of use of products furnished hereunder by any third party Mariner Dual 10 Port Switching Valve Assembly User Guide C 3 Appendix C Warranty Service Information Warranty The remedies provided herein are the buyer s sole and limitations C 4 Applied Biosystems exclusive remedies Without limiting the generality of the foregoing in no event shall Applied Biosystems be liable whether in contract in tort warranty or under any statute including without limitation any trade practice unfair competition or other statute of similar import or on any other basis for direct indirect punitive incidental multiple consequential or special damages sustained by the buyer or any other person whether or not foreseeable and whether or not Applied Biosystems is advised of the possibility of such damage including without limitation damage arising from or related to loss of use loss of data failure or interruption in the operation of any equipment or software delay in repair or replacement or for loss of revenue or profits loss of good will loss of business or other financial loss or personal injury or property damage No agent employee or representative of Applied Biosystems has any authority to bind Applied Biosystems to any affirmation representation or warranty concerning the product that is not contained in this Limited Warranty Statement Any such affirmation representation or warranty mad
48. ur d alimentation principal bouton poussoir Borne pouvant tre reli e la mise la terre d un autre appareil Ce n est pas une borne de mise la terre prot g e Borne de mise la terre de protection devant tre reli e la terre avant d effectuer tout autre raccordement lectrique a l appareil Borne recevant ou fournissant une tension ou un courant de type alternatif Borne pouvant recevoir ou fournir une tension ou un courant de types alternatif et continu Ce symbole appara t c t des valeurs des fusibles requis par le syst me gt a AOO AVERTISSEMENT Indique la pr sence d une haute tension et avertit l utilisateur de proc der avec pr caution Mariner M Dual 10 Port Switching Valve Assembly User Guide xiii Safety and EMC Compliance Information Symboles non Le tableau suivant donne la signification des symboles lectriques d alertes de s curit non lectriques qui figurent sur les appareils AVERTISSEMENT Avertit l utilisateur de la n cessit de consulter le manuel pour obtenir davantage d informations et de proc der avec pr caution AVERTISSEMENT Danger associ la pr sence d un appareil de chauffage Proc der avec pr caution pour viter de se br ler au contact de pi ces ou d l ments chauds AVERTISSEMENT Indique que l appareil renferme un laser BE gt Before operating Ensure that everyone invo
49. utilisez ce produit Un usage diff rent le syst me causer un accident ou endommager le syst me Mariner M Dual 10 Port Switching Valve Assembly User Guide xix Safety and EMC Compliance Information Safety and Electromagnetic Compatibility EMC Standards XX US Safety standards Canadian Safety and EMC standards European safety and EMC standards CE Applied Biosystems This instrument has been tested to and complies with standard ANSI UL 3101 1 Electrical Equipment for Laboratory Use Part 1 General Requirements 1st Edition It is an ETL Testing Laboratories listed product Safety This instrument has been tested to and complies with standard CSA 1010 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements It is an ETL Testing Laboratories listed product S curit Cet instrument a t v rifi avec la norme CSA 1010 Sp cifications de s curit du mat riel lectrique utilis pour les mesures les contr les et dans les laboratoires Partie 1 Sp cifications g n rales et il est conforme a cette norme C est un produit homologu par les ETL Testing Laboratories EMC This Class A digital apparatus meets all requirements of the Canadian Interference Causing Equipment Regulations Cet appareil num rique de la classe A respecte toutes les exigences du R glement sur le materiel brouilleur du Canada Sa
50. wing table summarizes the position of the valves at the completion of each stage Cycle Stage Valve 1 Position Valve 2 Position Start A A Event 1 B A Event 2 A B End A A This MS Method repeats the cycle for 10 minutes A 2 Applied Biosystems Creating the Example MS Method A 2 Creating the Example MS Method To create the example MS Method 1 From the Method menu in the Instrument Control Panel select New MS Method The MS Method Editor is displayed Figure A 1 2 Set Method Segment and Event Setting parameters as described in the following sections MS Method Editor C Mariner Program Methods contact switching msm M th d Method Setting ethno Total Run Time HH MM SS 00101 F Reset All Contact Outatend Total Number of Segments 1 gt r setting Ee r E E Segment Setting Segment 4 __ Duration HH MM SS 00 10 00 F Contact Out Set Event Count p setting ee eae z Event bar Event Setting Actions Branching Event a m a 5 V Instrument Setting Import BIC File Export BIC File View Edit Settings Always Go To Br anch i ng setting Sea 1 z Eventi ac tions Data Acquisition C Conditi dition Contact Out Set Repeat C Only Once Css nC When True Event Tag Seg zj Event 7 When False Types Set Seg y Event Save Save As Print Close F
51. y Valve Assembly Number Purchased Installation Process 5219 or later With instrument purchase Factory installed 5219 or later After instrument purchase You install following the directions in this document Earlier than 5219 After instrument purchase Installed by an Applied upgrade Biosystems Technical Representative 2 2 Applied Biosystems Before You Begin 2 3 Before You Begin Before you install the valve assembly you must e Read the appropriate safety information e Remove the original panels 2 3 1 Safety Information Before you install the valve assembly read the safety information in the front of the Mariner Workstation User Guide WARNING CHEMICAL HAZARD All chemicals used with the instrument including liquid in the lines are potentially hazardous Always determine what chemicals have been used in the instrument before changing reagents or instrument components Wear appropriate eye protection clothing and gloves when working on the instrument 2 3 2 Removing Original Panels Before you install the dual 10 port switching valve assembly you must remove the right front corner panel and inner rear panel If you have the optional integrated syringe pump installed the syringe pump is on the right front panel Mariner Dual 10 Port Switching Valve Assembly User Guide 2 3 Chapter 2 Installing the Valve Assembly Power down Remove front panel 2 4 Applied Biosystems
52. y ss C 2 Damages Claims Returns G 8 Spare Parts nement ee eee eee Index iv Applied Biosystems How to Use This Guide How to Use This Guide Purpose The Applied Biosystems Mariner Dual 10 Port Switching of this guide Valve Assembly User Guide describes the procedures for installing using maintaining and troubleshooting the dual 10 port switching valves with a Mariner Workstation Audience This guide is intended for novice and experienced Mariner Workstation users who utilize the optional dual 10 port switching valve assembly Structure The Applied Biosystems Mariner Dual 10 Port Switching of this guide Valve Assembly User Guide is organized into six chapters and three appendixes The table below describes the material covered in each chapter and appendix Chapter Content Chapter 1 The Dual 10 Port Switching Valve Assembly Chapter 2 Installing the Valve Assembly Provides an overview of the dual 10 port switching valve assembly and describes each of the components of the assembly Provides procedures for installing the dual 10 port switching valve assembly on a Mariner workstation Chapter 3 Connecting the Valve Assembly Chapter 4 Preparing the Valves Describes how to make signal connections from the valve assembly to the Mariner workstation or to an external device Describes how to make fluidic connections and test the valves Chapter 5 Controlling the 10 Port Switching
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