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Guide to Operations

1. 3 6 4 DATALOGGER See Section 3 7 for detailed information 3 6 5 POWER FREQUENCY You can adjust the power frequency to either 50 or 60 Hz to match the local electrical supply Use the 4 or P direction key until the correct frequency is displayed then press the ENTER function key 3 6 6 DISABLE This feature allows you to inform the control system to ignore certain sensors if their function is not required The standard item on this menu is the CO PRESSURE SWITCH for Auto Zeroing Additional Disable Options appear on this screen according to the options installed on your incubator See Section 8 1 for available options To disable a feature scroll to OFF using the 4 amp P direction keys then press the ENTER function key 3 6 7 DISINFECTION optional If the incubator is supplied with the High Temperature Disinfection option the menu item DISINFECTION will be displayed This feature activates the disinfection cycle of the incubator The disinfection cycle heats the inner chamber to 120 C holds that temperature for 4 hours then cools the chamber to the selected temperature setpoint All of the interior components with the exception of the O2 sensors if present can be left in place during the cycle to ensure that everything within the chamber is disinfected prior to resumption of activity For a full explanation of this feature see Section 8 5 1 3 7 DATALOGGER Screen The DATALOGGER Screen see Figure 13 on the follo
2. EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements Class 1 Installation Category Il Pollution Degree 2 EN 61010 2 010 2003 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 010 Particular requirements for laboratory equipment for the heating of material EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements Following the provision of 2006 95 EC The Low Voltage Directive and its amending directives 2004 108 EC The Electromagnetic Compatibility Directive and its amending directives This equipment should not be used in a residential area Mike King Managing Director o 27 March 2009 New Brunswick Scientific User s Guide 70 10 DRAWINGS amp TABLES 10 1 Index of Drawings Figure Title Page 1a Removable Lifting Handles 11 1 Inserting Shelf Racks 14 2 Fitting Tie Bar to Shelf Racks 14 3 Shelf Rack Assembly 15 4 Inserting Shelves 15 5 Installing the Humidity Tray 16 6 Humidity Tray Installed 16 7 Making Connections 17 8 AutoZero HEPA Filter amp Fitting 18 9 Control Panel 19 10 CO Incubator Rear View 20 11 USER SETTINGS Screen 23 12 PROGRAM CO AUTOZERO Screen 24 13 DATALOGGER Screen 26 14 ALARM EVENTS Screen 27 15 TEMPERATURE GRAPH DOOR OPEN BAR CHART Screen 28 16 CHAMBER ALARMS Screen 29 17 CHAMBER ALARM Message
3. New Brunswick aneppendorf company Guide to Operations Galaxy R Series 170 R CO Incubator MANUAL No CO170 R 0050 Revision B February 16 2010 New Brunswick Scientific PO Box 4005 44 Talmadge Rd Edison 08818 4005 USA 1 800 631 5417 1 732 287 1200 bioinfo nbsc com www nbsc com INTERNATIONAL OFFICES BELGIUM New Brunswick Scientific NV SA Stationsstraat 180 4 3110 Rotselaar Belgi Belgique Tel 32 0 16 56 28 31 Fax 32 0 16 57 27 53 E mail sales nbsnv sa be CHINA New Brunswick Scientific A903 Yin Hai Building No 250 Cao Xi Road Shanghai 200235 P R China Tel 86 21 6484 5955 or 5966 Fax 86 21 6484 5933 E mail nbsche online sh cn FRANCE Eppendorf France SARL 60 route de Sartrouville 78230 Le Pecq France Tel 33 0 1 30 15 67 40 Fax 33 0 1 30 15 67 45 E mail eppendorf eppendorf fr GERMANY Eppendorf Vertrieb Deutschland GmbH New Brunswick Produkte Peter Henlein Strasse 2 D 50389 Wesseling Berzdorf Deutschland Tel 49 0 2232 418 0 Fax 49 0 2232 418 155 E mail vertrieb eppendorf de THE NETHERLANDS New Brunswick Scientific BV Kerkenbos 1101 6546 BC Nijmegen Nederland Tel 31 0 24 3717 600 Fax 31 0 24 3717 640 E mail sales nbsbv nl UNITED KINGDOM New Brunswick Scientific UK Ltd 17 Alban Park Hatfield Road St Albans Herts AL4 OJJ United Kingdom Tel 44 0 1727 853 855 Fax 44 0 1727 835 666 E mail bioinfo nbsuk co uk Gal
4. We recommend that you repeat the OXYGEN SENSOR REF TO ATMOSPHERE procedure see Step 3 in Section 8 6 1 and further details in Section 8 6 4 once a month to ensure that any long term drift in sensor output will be corrected Be sure to do it at the chamber operating temperature When you are cleaning the chamber be very careful not to wet the oxygen sensor or sensor Never use solvents on the sensor membrane rather be sure to cap the hydrophobic filter before you clean In fact it is good practice to replace the filter each time you clean the incubator chamber to avoid the possibility of filter contamination Under normal relative humidity conditions 95 99 the oxygen sensor s performance should not be affected If however liquid condenses around the sensor gas flow may become restricted giving the sensor a low signal This may occur if there is a large liquid spill inside the chamber or if the incubator is turned off while it is fully humidified Should such condensation appear normal operation can be restored by e removing the humidity tray e drying the chamber completely then running the incubator at 37 C for one hour This will dry out the sensor s The humidity tray can then be reinstalled and the incubator can be safely re humidified New Brunswick Scientific User s Guide 54 8 6 4 Referencing to Atmosphere The oxygen sensor is a self powered electrochemical cell that has a finite life d
5. The design of the incubator ensures very low consumption of CO during normal working conditions a typical large cylinder should last approximately 12 months frequent door openings will deplete the supply more rapidly If there is a significant drop at the cylinder pressure of 725 psi or 50 bar it means that the cylinder is almost empty and should be replaced Ensuring that there are no leaks at any of the connections will ensure a greater lifetime to the supply and will help avoid accidentally running out of CO 3 Any spills in the chamber should be cleaned immediately to protect the stainless steel surfaces 4 Check the DATALOGGER screen for any alarms or events that may have occurred overnight 4 3 Weekly Checks Refill the humidity tray never exceed the maximum volume of 2 5 liters The use of warm water 37 0 C will ensure a rapid return to optimum chamber conditions 4 4 Monthly Check Remember that we recommend routine replacement of the water in the humidity tray and that you clean the tray at the same time Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 35 If required you can take a sample of the gas inside the chamber using the sample port and check it using a gas analyzer see Section 4 5 45 CO Sampling with Analyzer The sample port is located on the lefthand side of the incubator near the top see Figure 10 If you conduct a sampling please ensur
6. the sensor is unaffected If for any reason such as a large spill inside the chamber or the incubator being switched off while fully humidified liquid condenses around the sensor the result may be restriction of gas flow and a low sensor signal Should such condensation appear on the chamber walls normal operation can be easily restored by removing the humidity tray drying the chamber completely and then running the incubator at 37 C for one hour This will dry out the sensor After the hour has elapsed the humidity tray can be reinstalled and the incubator humidified again For routine service checks of the sensor see also Section 8 6 9 8 8 9 Specifications See Section 8 6 10 8 9 O2Sensor Replacement When the sensor needs to be replaced see Section 8 6 7 for instructions 8 10 IP66 Sealed Electrical Outlet Socket A WARNING Both the incubator and the IP66 enclosure must be plugged into an electrical supply protected by an RCD device Any device chosen must be a self resetting type which will automatically reconnect power to the incubator as soon as power is restored after a power failure The IP66 Sealed Electrical Outlet Socket is designed to provide a safe and convenient means of using electrically powered equipment within the incubator chamber The IP66 socket is powered on a completely separate circuit and therefore has its own plug lead and fuse New Brunswick Scientific User s Guide 66 The soc
7. Symbol for 4 CE Certification 69 Chamber Alarm Screen 30 Chamber Alarm System 30 Chamber Alarm to Check CO Supply 31 Checks Daily 34 Monthly 34 Cleaning 37 CO Control Specifications 40 Condensation 34 Minimizing 9 Control Panel 19 21 Cooling System 42 Copper Inner Chamber 68 Copyright Notice 4 D Datalogger 25 71 11 INDEX Diagnostic Graphs 28 Dimensions Specifications 40 Direction Keys 19 Disable Feature 25 Disclaimer Notice 4 Disinfecting 37 Disinfection Failure Code Explanations 49 Disinfection Failure Codes 49 Drawings Index of 70 Filter Disc Replacing the 56 Function Keys 19 Fuse Replacement 36 H Help Display 19 HEPA Filter 9 High Temperature Disinfection 48 Using the 48 with Oxygen Control 50 Humidity Control 10 Hydrophobic Filter 56 Replacement of 35 Replacing the 56 I Infrared Sensor 9 Inner Door 9 International Offices List of 2 IP66 Enclosure 65 IP66 Socket 65 L Location 13 M Manual Conventions 4 N Nitrogen Supply Connecting the 51 59 62 Nitrogen Tank Setting up the 51 58 62 NOTE Symbol for 4 O Control New Brunswick Scientific User s Guide 72 Maintenance 35 Operating Notes 34 Options 68 Factory Installed 42 Inner Glass Door options 68 Retrofittable in Field 42 Over Temperature Safety 10 Overview 9 Oxygen Control 0 1 19 58 60 0 1 1996 Alarm Impact 60 0 1 19 On Off 60 63 1
8. amp gauges controlling the gas supply are grease free for use with oxygen New Brunswick Scientific User s Guide 62 If your incubator is operating in the 3 95 range of control only the valve will operate for setpoints below 19 both the N2 and O2 valves will operate for setpoints between 19 and 80 and for setpoints above 80 only the O2 valve will operate NOTE If you plan to maintain O levels of 80 95 be sure to read Section 8 8 4 8 8 1 Setting Up the N Tank Before you set up your oxygen control be sure you have the proper equipment for your nitrogen supply 2 cylinders of nitrogen regulation size W two stage pressure regulator Inline pressure regulator 6mm PVC tubing tubing clips Set up the nitrogen tanks as follows 1 Inspect them to be sure there are no leaks or other damage 2 Check that the two stage pressure regulator valve and the inline pressure regulator valve are closed by trying to turn their knobs in a counter clockwise direction the knobs should be at the stop point Place the inline pressure regulator close to the incubator 4 Securely attach the two stage pressure regulator to the nitrogen tank s outlet and to the inline pressure regulator s inlet with tubing securing both ends with clips 5 Connect the inline pressure regulator s outlet to the incubator s N inlet on the right side of the equipment tray at the rear of the incubator see Figure 7 secure
9. 19 51 1 95 61 63 1 95 Alarm Impact 64 Alarms for 53 Enabling 52 Operating Guidelines for 53 Setting Up 51 Setting Up 0 1 19 59 Setting Up 1 95 62 Specifications 58 Oxygen Sensor Replacing the 55 Oxygen Sensor 50 Referencing to Atmosphere 52 53 54 Removing the 55 Oxygen Sensor Removal Tool 55 Oxygen Sensor Rear Access Cover 55 Oxygen Sensor Rear Access Cover 56 Oxygen Sensor Removal Tool 56 Oxygen Sensor Troubleshooting the 57 Oxygen Tank Setting up the 63 Power Frequency 25 Printer Port 68 Programmable CO AutoZero 24 Programming 21 R Referencing Oxygen Sensor 0 1 19 60 Relative Humidity Specifications 40 Remote Programming 46 Replace Sensor Now 54 Replace Sensor Soon 54 Restart Graphic Record 29 RS232 Interface 45 Setting Up Communications 33 45 RS232 Interface 33 RS232 Port 9 Sealing Inner Door 9 Sensor Cover 38 Sensor Removal Tool 55 Setting Up Nitrogen Tank 51 58 62 Setting Up Oxygen Tank 63 Setup 14 Setup Concerns Issues to Avoid 13 Shelves Specifications 40 Specifications 40 Oxygen Control 58 Table of Contents 6 Tables Index of 70 Temperature Graph Door Open Bar Chart 27 Temperature Management Specifications 40 U User Access Code 22 Lost Number 22 Setting the 22 USER Screen 23 User Settings 23 Utilities 12 WARNING Symbol for 4 Warranty 5 Weight Specifications 40 Galaxy R Series 170 R CO Incubator
10. 30 18 Chamber Alarm System Flow Chart 31 19 CHAMBER ALARM to Check CO Supply 32 20 DIAGNOSTICS Screen 32 21 HELP MENU Screen 33 22 BMS Relay Contact Alarm Socket 44 23 Selecting OXYGEN SENSOR REF TO ATMOSPHERE 52 24 USER SETTINGS Screen 52 25 Oxygen Sensor Rear Access Cover 55 26 Oxygen Sensor Removal Tool 56 27 Hydrophobic Filter amp Holder Assembly exploded view 57 28 IP66 Enclosure 66 29 IP66 Socket 68 10 2 Index of Tables Table Title Page 1 Accessories Provided 12 2 Utilities 12 3 Specifications 40 41 4 Cooling System Modes of Operation 43 5 Cooling System Troubleshooting Guide 43 6 BMS Relay Contact Alarm Socket Pin Designation 44 7a Disinfection Failure Codes amp Descriptions 49 7b Disinfection Failure Codes amp Explanations 50 Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 0 0 1 19 Oxygen Control 58 1 1 19 Oxygen Control 51 1 95 Oxygen Control 61 A Access Port 9 Adjusting Setpoints 21 Alarm Delay Programmable 30 Alarm Events 26 Alarm Events Screen 27 Alarm Options 29 Alarm Screen 29 Alarm Socket 44 Alarm Systems Specifications 40 Alarm Event Messages 47 Alarms 10 Audio Volume Adjust 23 AutoZero 9 Frequency 35 Auto Zero Check 22 AutoZero System Explanation of 24 B BMS Relay Contact Alarm 44 C CAUTION
11. 9 left most menu button 2 In the PROG screen that appears press the desired function key TEMP or CO then use the 4 amp P direction keys to adjust the value 9 the incubator is supplied with the option of oxygen control the setpoint for the oxygen level can be selected and changed like the temperature and CO setpoints 3 When the desired setpoint is displayed press the ENTER function key New Brunswick Scientific User s Guide 22 4 After making adjustments if any were made allow the incubator to stabilize at the setpoints before continuing NOTE If a temperature gt 1 C below the actual chamber temperature is set the independent over temperature system will activate This is indicated by a warning message on the display When the chamber cools down normal operation will resume Programming a higher temperature can also deactivate the warning 3 4 2 User Access Code A user access code is programmable if required The user access code allows you to restrict access to the PROG USER and ALARM screens where settings can be changed to authorized persons only To set the User Access Code 1 In the PROG screen accessed by pressing the PROG function key the user access code will be displayed as a series of four asterisks 2 Use the left and right direction keys to move to each code position and the up and down direction keys to select a number from 0 to 9 3 Once the number is sel
12. CO170R 0050 User s Guide
13. CO2 A Z SYSTEM INOPERATIVE AutoZero Event PROG CO2 A Z COMPLETED OK Door Event DOOR OPENED Door Event DOOR CLOSED After displaying any of the above messages a status report message relative to the alarm or event will be displayed e g DOOR EVENT DOOR OPENED Temperature Actual 37 0 C Setpoint 37 0 C CO2 Actual 00 1 Setpoint 05 0 New Brunswick Scientific User s Guide 48 8 5 High Temperature Disinfection The High Temperature Disinfection option is designed to heat the internal chamber to 120 C maintain that temperature for 4 hours and then allow the chamber to cool down to 37 C or the programmed temperature if different from 37 C when normal control takes over The cycle is designed to disinfect all internal surfaces and components with the exception of the Oxygen control sensor where supplied 8 5 1 Using High Temperature Disinfection 1 The incubator should be cleaned disinfected chemically and dried thoroughly before starting the cycle follow the instructions for Cleaning in Section 6 2 The black protective cover must be removed before starting a cycle the white porous cover can remain in place The shelves shelf racks humidity tray and silicone rubber feet and sleeves should all be in place during the cycle The incubator MUST be clean and dry and the humidity tray MUST be empty clean and dry to continue If it is not permanent damage can occur to the incubator that may void your warranty
14. Removable Lifting Handles 1 Rear of incubator CAUTION NEVER try to lift the incubator by its door this would cause permanent damage to the incubator New Brunswick Scientific User s Guide 12 Locate and remove the parts stored in the Humidity Tray If any part of your order was damaged during shipping is missing or fails to operate please contact your NBS distributor Using your NBS packing list verify that you have received the correct materials and that nothing is missing Table 1 outlines the accessory items that are supplied with your new incubator Table 1 Accessories Provided Quantity Item Notes 4 Screw in Lifting Handles Installed 4 Non tip Shelves Installed 2 Wire Shelf Racks Installed 1 Humidity Tray Installed 1 White porous CO Sensor Cover Installed 1 Black Sensor Cover Installed 1 Power Cord Packed in clear accessories bag 5 meters PVC Tubing 1 4 inch or 6mm l bore with an inline HEPA Packed in clear accessories bag 9 8 feet filter connected ready for use 2 Hose Clips Packed in clear accessories bag 1 Auto zero HEPA Filter Packed in clear accessories bag 2 Anti slip Pads for adjustable feet Packed in clear accessories bag 1 User Manual Provided There is a holder on the side of the incubator to store sensor covers see Figure 10 A WARNING Anytime you to
15. c Referencing the CO Sensor with AutoZero eerie eerte esses eene enata ettet tse ta etn sa sas t sese tasca 22 USER SettingS c sorsassnssssussnsonsnrsnsnnsnnsnsnnsnnne DATE TIME ADJUST AUDIO VOLUME ADJUST PROGRAMMABLE 24 DATALOGGER E RC utes E E AEA naeh in Deut QA ERE NE RENTE URINE EUER uS POWER FREQUENCY DIS BEE a ea e PE ptr en ete DISINFECTION optional 25 DATALOGGER qe 25 ALARM EVENTS TEMPERATURE GRAPH DOOR OPEN BAR 27 CO GRAPH DOOR OPEN BAR 28 DIAGNOSTIC CHAMBER ELEMENT 28 DIAGNOSTIC DOOR GRAPH nere ea an n EH eei aun cae DIAGNOSTIC DOOR ELEMENT GRAPH RESTART GRAPHICRECORD forces dB ERR NH EO Re eee ed eI CHAMBER ALARMS Menu Screen sssnsssssssnssnssssnssnsnnsnnsnsnnsnnsnssnsnnsnssnsnnsnnsnsnnsnnsnssnssnensanssnsnnsnnnnenn 29 Chamber Alarm System 30 DIAGNOSTIC Menu Screen eerie eres eee ee teste tentus 32 Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 4 1 4 2 4 3 4 4 4 5 4 6 5 1 6 1 6 2 6 3 8 1 8 2 8 2 1 8 3 8 4 8 5 8 5 1 8 5 2 8 6 HELP MENU Screen usuesesnsnenensenenennenenesnenenennenenssnensnennensnssnensnennenenssnenssennensnssnsnssennensns
16. could cause subsequent surface damage See the CAUTIONS in Section 6 2 6 2 Disinfecting The recommended disinfecting agent for use with the incubator is a solution of 70 isopropanol isopropyl alcohol and 30 distilled water Be sure to follow appropriate safety regulations while you are using this solution ZN WARNING As a routine precaution wear protective gloves Be sure to adequately ventilate the work area as you are disinfecting to avoid the formation of potentially explosive alcohol vapors Protect all electrical connections from contact with the alcohol solution To best protect yourself your incubator and your work area follow these instructions 1 Program 0 0 CO and switch off the incubator Unplug the incubator from the power supply 2 Dampen a clean cloth with the alcohol solution and wipe down all external surfaces taking care to keep the alcohol solution from coming into contact with any electrical outlets or assemblies New Brunswick Scientific User s Guide 38 3 Remove all of the shelves the humidity tray and the shelf racks 4 Place the black protective cover over the CO sensor Also protect any additional sensors such as Oxygen or Humidity with the cover s supplied CAUTION It is very important to ensure that no liquid is spilled onto the white porous CO sensor cover at the rear of the chamber Failure to use the protective cover s could result in damage to the
17. has a sealing inner door with a cam action lock to allow visualization of the cultures without compromising the internal atmosphere This is also available as a split 4 or 8 inner door option to coordinate with shelves which is ideal for critical hypoxic studies In addition there is a 25mm access port now standard to allow for seamless integration of independent probes or other equipment through the chamber Lastly NBS has included an RS232 port as standard on all Galaxy R series incubators This port will communicate with any computer through a hyperlink access or can be used to externally datalog the incubator through NBS software New Brunswick Scientific User s Guide 10 The incubator incorporates a two level alarm system The chamber monitoring alarms are programmable and will alert you if temperature or CO have not recovered within a preset time after the door has been opened If it is not required this system can be disarmed The system alarms occur only if a problem has developed with system components that require user intervention to rectify The incubator also incorporates an over temperature safety system that operates independently from the main control system The incubator features multiple options that can be installed to simplify maintenance and provide superior control over experimental conditions For example high temperature disinfection quickly and conveniently disinfects the incubator s chamber at 120 C without
18. instrument s outlet to the inline pressure regulator s inlet with tubing securing the ends with tubing clips o2 Setting Up O Control Oxygen control is tailored such that both the O and the CO levels are achieved at approximately the same time via control of the N valve s duty cycle but only within the range of 0 1 19 O 1 Remove the black plastic protective cover making sure that the hydrophobic filter cap is not removed with it from the port inside the chamber Retain the cover for use when you clean the chamber 2 Open the nitrogen gas supply from the tank and set tank s outlet pressure gauge to 1 5 bar 3 Setthe nitrogen regulator to 1 bar 14 5 psi The nitrogen will be fed into the incubator through a HEPA filter that is already installed in the gas line The gas flow rate is approximately 20 liters minute NOTE If the programmed O level is close to the ambient oxygen it may be necessary to reduce the cylinder pressure below 1 bar to stop the oxygen level from undershooting the programmed value NOTE When working at 0 1 O5 set the CO pressure to 0 2 bar 2 9 psi Be sure to humidify the incubator and leave it overnight to stabilize before proceeding further New Brunswick Scientific User s Guide 60 To automatically calibrate the sensor to atmospheric oxygen levels select OXYGEN SENSOR REF TO ATMOSPHERE in the USER menu and then follow the onscreen instructions The oxygen r
19. other For working continuously from 1 10 C below ambient temperature In this mode the cooling system operates as AMBIENT with the heating system switched off Before running the incubator with the cooling system option make sure the heat exchanger assembly cover is in place and make sure that the humidity tray is pushed fully back to keep deflector plate condensation from running down the rear of the tray onto the chamber base The heat exchanger cover can be removed for cleaning remove it from the four keyhole studs that hold it in place by gently lifting the cover out of the keyhold slots and then lifting it away toward you taking care not to force it as damage to the inner components could result Be sure to reinstall the cover with the same care before running the incubator again 8 2 1 Troubleshooting the Cooling System The thermo electric module which governs the cooling system is self contained and does not require any specific maintenance during the life of the incubator If however the cooling system fails to perform according to expectation consult the following Troubleshooting Guide Table 5 Cooling System Troubleshooting Guide Step Description Check that the incubator has been set to the desired cooling mode For the cooling system to operate at all either COOL or AMBIENT must be selected Check that the circulation fans are operating If either fan inside the chamber or outside at the
20. sensor may require recalibration to work properly in a completely dry atmosphere For humidified chamber with no CO the recommended maximum O level is 92 while for a humidified chamber with 5 CO we recommend no more than 87 When the incubator is running at normal humidity level 1 95 RH there is approximately 696 by volume water vapor present in the chamber If 596 is also present the maximum achievable level of O without using excessive quantities of oxygen is approximately 87 Oxygen direct from a cylinder has a dewpoint temperature of 45 C corresponding to 0 06 water vapor As it enters the incubator therefore its temperature is very low and even as it is warmed its RH level is very low Both of these factors affect the performance of the detector To minimize these effects O is fed continuously until it arrives at 0 4 below setpoint To allow the detector signal to recover and the RH level to build there is a 3 minute delay If O is still required it is fed in for 10 seconds with an additional 3 minute delay until setpoint is achieved As the humidity level rises the content in the chamber will diminish The use of will rise considerably as the setpoint approaches the sum contents of water wapor and the chamber In the short term it is possible to achieve higher levels of but as humidity rises and O content is pushed down there will be a continuous
21. socket is not in use Both the incubator and the IP66 enclosure must be plugged into an electrical supply protected by an RCD device Any device chosen must be a self resetting type which will automatically reconnect power to the incubator as soon as power is restored following a power failure note OTE If the incubator is to be used humidified the normal RH level is 95 so any electrically powered equipment or device to be used inside the chamber must be designed for use in a humid atmosphere Consult the manufacturer of the equipment to ensure that it is suitable To minimize the thermal gradient within the chamber the device should be placed on the bottom shelf The heat dissipated by the equipment should be as low as possible to minimize thermal disturbance to the chamber Some shakers stirrers etc have been specifically designed to give a low heat output for use in incubators The maximum heat that can be dissipated within the chamber is variable depending on the difference between the ambient and programmed temperatures If the heat dissipated is too great the chamber will tend to overheat If this happens the control parameters can be adjusted to compensate 0 Any heat generating equipment used inside the chamber will naturally affect the temperature performance of the incubator For this reason if you plan to use equipment which may generate heat in the chamber we recommend equipping the incubator
22. the PROG screen following the onscreen instructions If you are running an O level programmed between 0 1 0 9 0 you should know that the control system is set to operate in the following way to minimize consumption after the glass door has been opened The valve is switched on continuously until the level is within 0 196 of setpoint Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 61 e The CO valve is then switched on to allow the CO level to reach setpoint If the level is above setpoint 15 minutes after the valve has been switched off it is switched back on for 40 seconds and the CO valve is switched on for 20 seconds The CO valve will then pulse until setpoint is reached e The process described above will repeat itself until the O setpoint is reached The same process will also repeat if the Oz level rises above setpoint and if the O level should rise toward 0 2 above setpoint the valve will open again continuously until the O level returns to setpoint e The CO AutoZero which would normally take place after a alarm will be cancelled to avoid the introduction of additional O into the chamber For the same reason we recommend canceling the programmed CO AutoZero 8 7 7 Precautions Under normal humidity conditions 95 9996 RH the sensor is unaffected If for any reason such as a large spill inside the chamber or the incubator being switched off wh
23. the chamber completely and then running the incubator at 37 C for one hour This will dry out the sensor After the hour has elapsed the humidity tray can be reinstalled and the incubator humidified again Change the hydrophobic filter each time you clean the incubator to avoid contamination or clogging over time New Brunswick Scientific User s Guide 36 5 SERVICE 5 1 Fuse Replacement To replace a fuse you will need an ordinary flat bladed 5mm maximum screwdriver 1 2 3 Using the flat bladed screwdriver rotate the fuse holder see Figure 7 for location counter clockwise until the fuse holder springs out Remove the spent fuse Install a new fuse make sure it is of the same type Orient the fuse holder back in the side panel and using the screwdriver as before secure it in place Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 37 CLEANING amp DISINFECTING 6 1 Cleaning 1 Routinely clean the exterior of the incubator by wiping it over with a soft cloth moistened with soapy water 2 Rinse the soap from the cloth in clean water and wipe the exterior surfaces again CAUTION Be sure to use only approved cleaning fluids and materials Solvents chloride based cleaning substances and abrasive materials among others may cause permanent damage to the product surfaces Also be sure to wipe all surfaces dry leaving them free from any foreign particulates or fluids which
24. the need to remove interior components or the CO sensor A humidity tray warning system warns the user before the humidity tray runs out of water preventing dehydration of samples Oxygen control provides for conditions that require above or below ambient oxygen levels These and other options and accessories provide for a uniquely flexible CO incubator capable of meeting the most demanding requirements Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 11 2 UNPACKING amp INSTALLATION 2 1 Inspection of Boxes After you have received your order from NBS inspect the boxes carefully for any damage that may have occurred during shipping Report any damage to the carrier and to your local NBS distributor immediately 2 2 Unpacking CRUSH WARNING At least four people are required to safely lift your Galaxy 170 R Disassemble the shipping crate and remove the protective packing Save the packing materials for possible future use and be sure to save this User s Guide for instruction and reference User manuals can also be found online in PDF format at www nbsc com To simplify lifting the incubator leave it on the pallet as you install the four lifting handles provided see Figure la by screwing them into the tapped holes on both sides You must have at least two persons at either side to safely lift the incubator Supported by the lifting handles only lift the incubator from the delivery pallet Figure 1a
25. 1 OF SET le 6 i _ 15 MINUTES ALARM DELAY TIMEOUT STARTED YES M 15 MINUTES 5x ALARM DELAY TIMEOUT COMPLETED 31 VISIBLE AND AUDIBLE SCREEN CANCEL ALARM BY gt r ALARM MESSAGE ON PRESSING ANY KEY CANCELLED lt HAVE CONDITIONS VISUAL AND AUDIBLE RECOVERED TO ALARMS CANCELLED J WITHIN 0 12 MESSAGE LEFT ON SCREEN PRESSING MESSAGE BY If the CO valve is opened and no pressure is detected an alarm occurs and a warning message appears on the screen alerting you to CHECK CO SUPPLY see Figure 19 on the following page New Brunswick Scientific User s Guide 32 Figure 19 CHAMBER ALARM to Check CO Supply O2 LEVEL B ROGRAMMED SUBE 0 02 As you can see in Figure 19 above instructions are provided in the ALARM screen to deal with the situation that triggered the alarm 3 9 DIAGNOSTIC Menu Screen The diagnostics screen Figure 20 below contains technical information regarding the status of many of the system components found on the incubator This screen is mainly for technical service use and can be used to troubleshoot the incubator systems before service is scheduled This information allows technical support to optimize the service support required and to shorten service time
26. 3 To start the cycle press the USER menu button select DISINFECTION and press START The incubator will then prompt IS CHAMBER CLEAN amp DRY Answer YES if it is clean and dry The cycle will start automatically unless the incubator is fitted with Oxygen Control in which case the incubator will also prompt IS 02 SENSOR REMOVED Make sure that the O sensor has been removed and answer YES to begin the cycle See Section 8 5 2 for the O2 sensor removal reinstallation procedure A WARNING During the High Temperature Disinfection cycle the outer surfaces of the incubator will get hot and should not be touched 4 To cancel the cycle press CANCEL The incubator will cool down to the programmed level where normal control takes over 0 In the unlikely event of a scheduled AutoZero beginning just prior to a disinfection cycle the AutoZero will abort until the cycle is complete A user initiated AutoZero will also abort but will not resume after completion of the disinfection cycle 5 If the incubator door is opened during a disinfection cycle the process will continue as normal but will cause a failure message if the temperature falls as a result during the heating phase and especially during the disinfection phase See important WARNING on the following page Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 49 WARNING The door should NOT be opened during the cycle due to the hazardous h
27. 30V Fuses 10A BA 0 NOTE Maximum operating ambient temperature is 35 C Factory calibration of the incubator is carried out at 37 C 5 0 CO and 90 to 95 RH in an ambient temperature of 20 25 C with no heat generating apparatus inside the chamber Software calibration adjustments may be required to optimize performance if the incubator is being used well outside these operating conditions Performance specifications may also be affected For advice on calibration adjustments and relevant performance specifications contact NBS Please be prepared with the model and serial number of your incubator and the complete details of your operating conditions co RECOVERY AFTER 15 SEC DOOR OPENING TEMPERATURE 375 RECOVERY AFTER 15SEC DOOR OPENING New Brunswick Scientific User s Guide 42 8 8 1 Available Options OPTIONS amp ACCESSORIES Some option combinations are not possible others may incur extra cost Please inquire before ordering Field Options NBS Part Number Retrofitable Cooling System P0628 6810 No BMS Relay P0628 5540 Yes High Temperature Disinfection CO170 R 1000 No Control 1 19 CO170 R 0200 No O2 Control 0 1 19 P0628 5410 No O Control 1 195 P0628 5400 No IP66 Power Receptacle P0628 5612 No Copper Chamber P0628 5612 No 4 Split Inner Glass Door P0628 6780 Yes 8 Split Inner Glass Door P0628 6781 Yes Humidity Package
28. Disinfection cycle Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 51 8 6 Control 1 19 This oxygen control option is designed to cover the 1 19 range by adding nitrogen to bring the level below ambient f you have the 0 1 19 oxygen control option skip ahead to Section 8 7 or if you have the 1 95 oxygen control option skip to Section 8 8 8 6 1 Setting Up the N Tank Before you set up your oxygen control be sure you have the proper equipment for your nitrogen supply 2 cylinders of nitrogen regulation size W two stage pressure regulator Inline pressure regulator 6mm PVC tubing tubing clips Set up the nitrogen tanks as follows 1 Inspect them to be sure there are no leaks or other damage 2 Check that the two stage pressure regulator valve and the inline pressure o2 6 9 regulator valve are closed by trying to turn their knobs in a counter clockwise direction the knobs should be at the stop point Place the inline pressure regulator close to the incubator Securely attach the two stage pressure regulator to the nitrogen tank s outlet and to the inline pressure regulator s inlet with tubing securing both ends with clips Connect the inline pressure regulator s outlet to the incubator s inlet on the right side of the equipment tray at the rear of the incubator see Figure 7 secure both ends with clips Proceed as indicated in Section 8 6 2 To ad
29. Enabling or Disabling O Control Sd ase ed ove de DIO RUBRO m Om dad 8 7 5 Referencing to Atmosphere 8 7 6 Programming Desired 8 57 Preca ttoris an Ernie EISEN 8 8 P 8 81 Setting Up the No Tank ene terere e nbi e e D a rS I iens 8 8 2 Setting Up O Control eee aen eR Cede e D ie ER He PES 8 8 3 Enabling or Disabling 8 8 4 Important Notes for O Levels gt 80 8 85 a E n a cet pe ER PB HET AT rre ir Up In mee qe 8 8 6 Referencing to Atmosphere sese ettet et e UO Die ee repe ee dete b eere tends 8 8 7 Programming Desired O 888 Other Precautions n RR ERE e tle ea a ee 8 8 9 Specifications 25e tim m dees DE EN EE ERE RR COT ER EO ERU REIR ERES 8 9 Sensor Replacement saser sesoses sosise sosetan toese eeso eeso 65 8 10 IP66 Sealed Electrical Outlet Socket ssccsssssssssesscsssssessseesssssassssesessssssessssssessssesessseesesesseseserees 65 8 11 Copper Inner Chamber saen es seseo s orisek esos 68 8 12 Other Om C reias 68 8 13 Available 68 9 CE CERTIFICATION u aan 69 10 DRAWINGS amp TABLES 70 10 1 Index of Drawings cusse
30. Figure 20 DIAGNOSTICS Screen 30110115 04 TED CLOSED LMT Lh E HL TER TAG 3 10 HELP MENU Screen The HELP MENU screen Figure 21 on the following page provides user selectable categories of abbreviated information found in the user manual All the major systems are covered in the help menu including help on installing the incubator If the user manual is misplaced information about the incubator and its functions can always be found on screen Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 33 Figure 21 HELP MENU Screen HELP MENU Ml USIHG 4 crn HI 3 11 RS232 Interface The RS232 interface port provides a connection for the incubator to a personal computer or terminal This allows the incubator to be programmed remotely and the incubator s operating status to be viewed on a remote screen In addition to the incubator and this port you will need the following A PC or terminal capable of RS232 communication with one serial com port free for this connection A null modem screened cable of suitable length see NOTE below To ensure reliable communication the cable should not exceed 49 feet 15 meters in length One end must have a 9 pin female D connector to connect to the incubator s RS232 port and the other end must have either a 9 pin or 25 pin female D connector whichever mates to your PC or terminal NOTE T
31. P0628 6820 No 8 2 Cooling System Option The cooling system option allows the incubator to be used in one of three possible modes as explained in Table 5 on the following page This option consists of a heat exchanger assembly fitted to the rear wall of the chamber with a circulation fan Inside the incubator chamber the interior cooling fins and fan are enclosed by a removable cover with slots for airflow to the fan and the shelves To cool the chamber the fan on the heat exchanger assembly circulates chamber air across the cooling fins of the heat exchanger Outside the chamber the rear of the heat exchanger assembly has an external cooling fan to cool the exchanger s hot fins by blowing ambient air across them The three modes of operation accessible in the USER menu under COOL OPTIONS are explained in Table 4 Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 43 Table 4 Cooling System Modes of Operation Mode Description NORMAL For working at 37 C in average ambient temperatures of 20 30 C In this mode the fans and heat exchanger assembly are switched off and the heating system operates as standard without using the cooling option AMBIENT For working at ambient temperature therefore maintaining the COOL chamber temperature within a close range of the outside air In this mode the fans amp heat exchanger and the heating system are switched on to balance each
32. S ZERO KEY TO CARRY OUT A Z NOW MNUFS CURSOR 4 ADJUSTS VALUE ENTER 9 This procedure does not affect the internal chamber environment and will not affect your cell culture as it is being performed 2 Atthe end of the countdown the control system adjusts the AutoZero Factor to reference the sensor to 0 05 CO which is the approximate atmospheric level 3 The pump switches off and the chamber atmosphere diffuses back into the sensor s measuring chamber This takes three minutes after which the normal CO control system takes over 4 The result of the AutoZero listed as A Z on some screens is sent to the DATALOGGER ALARM EVENTS screen so that a record of the results will be kept The frequency of AutoZeroing can be set in steps between once a day and once every 28 days which is the default setting If not required it can be disabled see Section 3 6 6 The default time setting is 7 00 am This can be altered to suit your requirements We do recommend that you only change the time setting shortly before you start to use the incubator Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 25 9 The AutoZero will only occur if the temperature is at setpoint If the temperature is not at setpoint the system will postpone AutoZero until the setpoint is achieved If the AutoZero function is to be run manually simply press the START function key within the PROGRAM CO AUTOZERO window
33. The CO system has a programmable automatic zero system Auto zero to re reference the sensor baseline to atmospheric CO levels at regular intervals A small pump supplies HEPA filtered atmospheric gas to the sensor The chamber atmosphere within the sensor is completely displaced allowing the control system to automatically reference the sensor after which the pump is switched off allowing the chamber atmosphere to homogenise back into the sensor This provides for accurate CO control without disturbing the chamber environment An independently controlled water tray at the bottom of the incubator allows a high uniform relative humidity while preventing condensation in other parts of the chamber Perforated shelves are provided as standard to facilitate a much faster recovery of RH conditions in the chamber than with unperforated shelves The 170 liter chamber is seamless to provide a sanitary and easy to clean environment and all internal components are manufactured from polished stainless steel The shelves which are non tip shelf racks and humidity tray are easily removed without tools for thorough cleaning and are capable of being sterilized Air circulation is achieved without the use of a fan eliminating ductwork a potential source of contamination simplifying cleaning eliminating vibration and reducing small sample evaporation within the chamber The Galaxy 170 R model contains many standard features usually seen as options It
34. WARNING Before making electrical connections verify that your power supply voltage matches the voltage of your incubator and that the on off switch is in the OFF position Figure 7 Making Connections 1 ON OFF switch 5 Fuse holder 2 Optional O2 inlet 6 Righthand side of equipment tray 3 inlet 7 Power cord receptacle 4 Optional inlet 8 Label Install the Auto Zero HEPA filter press the Auto Zero HEPA filter gently into the white plastic filter socket see Figure 8 on the left side of the equipment tray New Brunswick Scientific User s Guide 18 Figure 8 AutoZero HEPA Filter amp Fitting 1 RS232 socket 4 AutoZero HEPA filter 2 Optional printer port location 5 Lefthand side of equipment tray 3 Optional BMS relay contact alarm socket see Section 8 3 for details IN WARNING Before making electrical connections verify that your power supply voltage matches the voltage of your incubator and that the on off switch is in the OFF position Install the power cord 1 Insert the power cord into its receptacle on the right side facing the incubator of the equipment tray see Figure 7 on the previous page 2 After verifying that your supply volt
35. age matches the voltage specified for your incubator and that the ON OFF switch is OFF plug the cord into your power supply outlet Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 19 3 OPERATION 3 1 Control Panel The control panel consists of an LCD display five function keys amp four direction keys see Figure 9 Figure 9 Control Panel 1 Function key menu 3 Direction keys 2 Function keys The illustration above shows the screen in normal operation This is also how the screen should look when you switch the incubator on The purpose of each Function Key is identified at the bottom of the display above the corresponding key the function may change from screen to screen The cluster of four arrowed Direction Keys will move the cursor around the screen and adjust values The HELP file contains most of the information in this User Manual together with more detailed troubleshooting information New Brunswick Scientific User s Guide 20 3 2 Preparing for Operation Remove the black protective cover from the CO sensor taking care not to remove the white porous cover store the black cover on the holder on the side of the equipment tray see Figure 10 below The sensor cap should be placed back on the sensor when the incubator is to be cleaned Figure 10 Incubator Rear View Access port 4 sample po
36. ap out of the holder Clean and dry the filter holder and cap Wearing gloves to avoid contaminating the filter disc gently place the new filter membrane disc into the filter holder recess The filter disc works in both directions so there is no right or wrong side Figure 27 Hydrophobic Filter amp Holder Assembly exploded view 1 Oxygen sensor holder 4 Hydrophobic filter holder 2 Oxygen sensor 5 Hydrophobic filter cap 3 Hydrophobic filter disc 5 Make sure the O rings between the cap amp filter holder and between the filter holder amp oxygen sensor holder are undamaged and securely in place 6 Gently press the filter cap back in 7 Press fit the filter assembly back onto the oxygen sensor holder 8 6 9 Troubleshooting the Oxygen Sensor If the oxygen sensor fails suddenly it is very likely that the sensor inlet membrane has become blocked by condensation This can be seen on the DATALOGGER screen as a sudden drop from the programmed value to nearly zero To dry the membrane l Remove the hydrophobic filter holder assembly see Figure 25 by unscrewing it counter clockwise New Brunswick Scientific User s Guide 58 2 Program the incubator for a temperature of at least 37 C or higher if you normally operate the incubator at a higher temperature Close the door and allow the temperature to recover Reopen the door for 15 seconds to release any build up of humidit
37. axy R Series 170 R CO Incubator CO170R 0050 User s Guide CAUTION This equipment must be operated as described in this manual If operational guidelines are not followed equipment damage and personal injury can occur Please read the entire User s Guide before attempting to use this incubator Do not use this equipment in a hazardous atmosphere or with hazardous materials for which the equipment was not designed New Brunswick Scientific NBS is not responsible for any damage to this equipment that may result from the use of an accessory not manufactured by NBS New Brunswick Scientific User s Guide Copyright Notice New Brunswick Scientific Box 4005 44 Talmadge Road Edison New Jersey 08818 4005 Copyright 2010 New Brunswick Scientific All Rights Reserved Reproduction adaptation or translation without prior written permission from New Brunswick Scientific is prohibited Disclaimer Notice New Brunswick Scientific reserves the right to change information in this document without notice Updates to information in this document reflect our commitment to continuing product development and improvement Manual Conventions 0 NOTE Notes contain essential information that deserves special attention Caution messages appear before procedures which if CAUTION caution is not observed could result in damage to the equipment N Warning messages alert you to specific procedures or WARNING practic
38. both ends with clips 6 Proceed as indicated in Section 8 8 2 note OTE To add an automatic gas cylinder changeover instrument with the incubator turned off connect its inlet to the two stage pressure regulator s outlet and the changeover instrument s outlet to the inline pressure regulator s inlet with tubing securing the ends with tubing clips o2 8 8 2 Setting Up O Control Oxygen control is tailored such that both the and the CO levels are achieved at approximately the same time via control of the O2 valve s duty cycle Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 63 1 Remove the black plastic protective cover making sure that the hydrophobic filter cap is not removed with it from the port inside the chamber Retain the cover for use when you clean the chamber 2 Open the nitrogen gas supply from the tank and set tank s outlet pressure gauge to 1 5 bar 3 Set the nitrogen regulator to 1 bar 14 5 psi but be sure to read the NOTE below The nitrogen will be fed into the incubator through a HEPA filter that is already installed in the gas line The gas flow rate is approximately 20 liters minute 4 Install the oxygen cylinder in the same manner as outlined for the nitrogen cylinder installation in Section 8 8 1 using the incubator s O2 inlet of course For this application you will need another two stage pressure regulator and another inline press
39. ct the desired delay 15 minutes in the sample screen above to allow for temperature and recovery after the door has been opened Alternatively the alarm system can be set to rearm only after the original temperature and setpoints have been achieved 1 Choose the option ARM ALARMS WHEN AT SETPOINT 2 Select YES for both TEMP and CO2 3 When YES is selected for this function the DELAY IN ARMING AFTER DOOR OPEN is ignored A DOOR OPEN ALARM can be adjusted choosing from seven preset durations 45 seconds in Figure 16 to warn of an improperly closed door The AUDIBLE and VISUAL alarms can be adjusted from OFF to ON which means the alarm will be on continuously until it is acknowledged in seven preset time increments New Brunswick Scientific User s Guide 30 In the OFF position any Chamber Alarms that occur will be displayed on the screen without flashing and with the audible alarm inhibited Section 3 8 1 Chamber Alarm System Function and Figure 18 explain in detail how the Chamber Alarms operate 3 8 1 Chamber Alarm System Function When the incubator is switched ON or after the temperature and CO levels have been reprogrammed the alarm system is inactive until the setpoint values 0 1 are achieved after which the alarm system is armed As setpoint is achieved the CO and temperature alarms are individually armed If temperature and or CO levels deviate more than the programmed setpoints
40. d an automatic gas cylinder changeover instrument with the incubator turned off connect its inlet to the two stage pressure regulator s outlet and the changeover instrument s outlet to the inline pressure regulator s inlet with tubing securing the ends with tubing clips 8 6 2 Setting Up Oxygen Control 1 Remove black plastic protective cover from hydrophobic filter cap making sure that the hydrophobic filter cap is not removed with it located in the rear wall of the incubator chamber Retain the cover for use when you clean the chamber Open the nitrogen gas supply from the tank Set the tank s outlet pressure gauge to 1 5 bar New Brunswick Scientific User s Guide 52 3 Setthe nitrogen regulator to 1 bar 14 5 psi The nitrogen will be fed into the incubator through a HEPA filter that is already installed in the gas line The gas flow rate is approximately 20 liters minute 9 the programmed O level is close to the ambient oxygen it may be necessary to reduce the cylinder pressure below 1 bar to stop the oxygen level from undershooting the programmed value 4 After the incubator has been humidified and left overnight to stabilize select the USER menu then using the A or direction key select OXYGEN SENSOR REF TO ATMOSPHERE see Figure 23 and follow the onscreen instructions to automatically calibrate the oxygen sensor to atmospheric oxygen levels The oxygen reading is automatical
41. d in Section 3 11 the RS232 interface port provides a connection for the incubator to a personal computer or terminal This allows the incubator to be programmed remotely and the incubator s operating status to be viewed on a remote screen 9 The computer should be connected to a power supply outlet as close as possible to the incubator to avoid grounding problems To use the RS232 interface 1 Connect the null modem cable to the RS232 port at the rear of the incubator see Figure 8 Connect the other end of the cable to either the 25 pin male D serial com port or the 9 pin male D serial com port on the PC or terminal Turn on the computer and start the communications software Select an unused serial port to enable communications between the incubator and the PC or terminal f you know which port to use continue to Step 5 and then skip to Step 7 If not continue to Steps 5 amp 6 Using the communications software select the unused port COMI COM2 etc then press the J Enter key on the PC terminal keyboard If you have successfully chosen an unused port the message Type for help should appear on the computer screen If you see any other message or nothing happens select the next port and press the J Enter key again If you still do not see the expected message change the Flow Control setting from Hardware to None then try again When the connection between the incubator and PC terminal has b
42. d or altered outside the NBS factory without prior authorization from the New Brunswick Scientific New Brunswick Scientific User s Guide 2 1 2 2 2 3 2 4 2 5 2 6 3 3 1 3 2 33 34 3 4 1 3 4 2 3 5 3 6 3 7 3 7 1 3412 3 7 3 3 7 4 3 75 3 7 6 3 7 7 3 8 3 8 1 3 9 TABLE OF CONTENTS Lxiep isi eR 9 UNPACKING amp 11 Inspection of C 11 Unpacking rJ 11 j nger o 12 Location 13 Setting jj 13 Making Connections ictss secc ieccectesccasevesecsensturseteccessoncvedcsnssescsnceasbectesedsecevssdeoscnscvesenesecsenteussesuscecccessseesnsss 16 OPERATION M 19 Control Panel m 19 Preparing for 20 Using the Humidity Tray amp Humidity Control ssssesssseesososesesososeeososoeseososseoeosossesoeeesesooeeesosoeesesosee 21 E SSeS oe asss SSES Temperature and CO Level vuU qas
43. demand for and RH will remain too low At the same time as O2 is pumped in the level will also diminish causing the system to add CO which in turn causes the level to drop again If the setpoint is too high the incubator can consume some 2 500 liters of per day which does not include the extra oxygen consumed each time the door is opened about 500 liters 8 8 5 Alarms The alarm levels are set automatically to 0 5 above and below the programmed value but these points can be altered in the ALARM screen In addition the rearming of the alarm can be delayed until the Programmed Value is achieved by selecting the relevant option in the ALARM screen For example it is possible at low oxygen levels that the CO and levels might not fully recover within the Alarm Limits after the 15 minute DELAY IN ARMING AFTER DOOR OPENING period has elapsed so this time period can be increased in the ALARM screen to suit individual circumstances Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 65 8 8 6 Referencing to Atmosphere 9 It is highly recommended to reference the sensor to atmospheric oxygen at least once per month See Section 8 6 4 for details on this important procedure 8 8 7 Programming Desired O Level Program the required oxygen level in the PROG screen following the onscreen instructions 8 8 8 Other Precautions Under normal humidity conditions 95 99 RH
44. e incubator As with the program commands these commands are not case sensitive lower case or upper case letters work exactly the same way The following are examples of how each of the Status commands might be used S S tatus report Type S then press the Enter key to display a current status report which may look like this Temperature Actual 37 0 C Setpoint 37 0 C CO2 Actual 05 0 Setpoint 05 0 SInn nn S tatus report at I nterval of n minutes n seconds Type SI60 0 then press the Enter key to display a current status report as shown above every hour A confirmation message will appear Status report at interval 60 0 will be given Press Enter or ESC to stop reports Other Commands As with the Program and Status commands the V Version number report and Help commands are not case sensitive To use these two commands V Type V then press the Enter key to generate a report on the current firmware version 2 Type then press the 1 Enter key to return to the initial help screen Alarm Event Messages a number of messages are preset to appear on the computer screen to inform you of either an alarm condition or a certain event The following table recaps those messages Type of Message Message Alarm TEMPERATURE LOW Alarm TEMPERATURE HIGH Alarm CO2 LOW Alarm CO2 HIGH Alarm TEMPERATURE SENSOR FAILURE Alarm AutoZero PROG
45. e the following Turn off the CO gas by re programming the setpoint for CO to 0 0 to prevent CO from being injected into the chamber and giving a false reading A flow rate lt 0 5 liters minute is used to take a sample door is kept closed to minimize heat loss Remember to reset the CO setpoint to the desired level after sampling We recommend that you perform a CO AutoZero prior to sampling see Section 3 5 9 Displayed chamber CO level will drop during sampling but it will recover once the sampling is complete This is merely a sensor characteristic the CO level in the chamber is actually not affected We also recommend that you AutoZero the CO system at least once every 28 days to ensure that level is correct 4 6 Routine Checks of the O2 Control Option Be sure to conduct the reference to atmospheric oxygen procedure on a monthly basis to ensure that long term drift in output from the sensor will be corrected and to determine when the sensor requires replacement Under normal humidity conditions 95 9996 RH the sensor is unaffected If for any reason such as a large spill inside the chamber or the incubator being switched off while fully humidified liquid condenses around the sensor the result may be restriction of gas flow and a low sensor signal Should such condensation appear on the chamber walls normal operation can be easily restored by removing the humidity tray drying
46. eading is automatically adjusted to 19 7 which is the true reading taking into account the Relative Humidity in the chamber 8 7 3 Enabling or Disabling O2 Control To enable oxygen control 1 Select the USER screen by pressing the USER function key 2 Use the direction keys to select MANUAL DISABLE 3 Press the ENTER function key then use the direction keys to select ON for Oxygen Control 4 Press the ENTER function key again Program the required oxygen level in the PROG screen following the onscreen instructions To disable oxygen control follow the pattern used to enable it but this time select USER MANUAL DISABLE ENTER OFF and then ENTER again 8 7 4 Alarms The alarm levels are set automatically to 0 5 above and below the programmed value but these points can be altered in the ALARM screen In addition the rearming of the alarm can be delayed until the Programmed Value is achieved by selecting the relevant option in the ALARM screen For example it is possible at low oxygen levels that the and levels might not fully recover within the Alarm Limits after the 15 minute DELAY IN ARMING AFTER DOOR OPENING period has elapsed so this time period can be increased in the ALARM screen to suit individual circumstances 8 7 5 Referencing to Atmosphere To reference the oxygen sensor to atmospheric oxygen levels see Section 8 6 4 8 7 6 Programming Desired O Level Program the required oxygen level in
47. ected press the ENTER function key to save the code 4 After returning to the main screen programming access will require the code to make any further programming changes To cancel the access code 1 In the PROG screen enter the current access code 2 Now program 0000 as the new access code 3 Press the ENTER function key to save the change 4 The code is now cancelled and programming is no longer restricted If the access code has been misplaced you will be unable to make changes to your incubator s settings Contact customer service or your service representative for instructions on how to regain access to your incubator 3 5 Referencing the Sensor with AutoZero Prior to using the incubator you should manually perform a AutoZero see Section 3 6 3 for an explanation of this feature Perform a CO AutoZero by pressing the USER function key see Figure 9 selecting the PROGRAMMABLE CO2 AUTOZERO and pressing the START key Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 23 2 The incubator will display a countdown as the AutoZero is running 3 When the countdown is complete the incubator is ready to use 9 may be necessary to open the door momentarily if after performing an AutoZero the CO level is too high 3 6 USER Settings In the USER screen see Figure 11 below you can adjust the features called out on the screen Figure 11 USER SETTINGS Scree
48. ed on the incubator The system is connected at the rear of the incubator via a standard 6 pin DIN socket see Figure 10 for location amp Figure 22 for a detailed look The matching plug is provided when the option is installed Figure 22 BMS Relay Contact Alarm Socket Table 6 BMS Relay Contact Alarm Socket Pin Designation Pin Designation 1 24VDC unregulated To power external equipment such as a remote buzzer 2 OV or light 100mA maximum current available 3 5VDC Via 10KQ pull up resistor for a logic signal to directly control an auxiliary control system s Normally dosed To access the relay contacts Contact limits are 3 Amps 5 Common 6 Normally open 24V DC and 3 Amps 34 VAC Cable length should not exceed 3 meters 9 8 ft to comply with EMC requirements The default setting for the alarm system is ON To deactivate the relay using the incubator keypad 1 Press USER 2 Select BMS ALARM RELAY 3 Select MAKE ALARM RELAY ACTIVE YES NO 4 Toggle to NO and then press ENTER Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 45 The default setting for the power failure warning is ON To make the alarm system ignore any power outage intentional or not 1 3 4 Press USER Select BMS ALARM RELAY Select MAKE ALARM RELAY ACTIVE AT POWER SWITCH OFF FAILURE YES NO Toggle to NO and then press ENTER 8 4 RS232 Interface As indicate
49. een successfully established and the message indicated in Step 5 appears type as prompted by the message onscreen a in the communications software then press Enter key The menu shown on the following page should now appear on the computer screen New Brunswick Scientific User s Guide 46 zi 3 PTnn n Galaxy R Incubator Command Help P rogram T emperature to nn n degrees P rogram C 02 to nn n percent P rogram A larm T emperature H igh to n degrees P rogram A larm T emperature L ow to degrees P rogram A larm C 02 H igh to n percent P rogram A larm C 02 L ow to percent L ist D atalogger L ist D atalogger T emperature alarms L ist D atalogger C 02 alarms L ist D atalogger P ower on off events S tatus report S tatus report at I nterual nn nn mins secs U ersion number report Gives this help screen Connected 00 00 08 ANSIW 19200 8 N 1 NUM This menu allows the incubator temperature CO and alarm setpoints to be programmed remotely A running status report can be generated at user defined intervals and all reports can be sent to a printer For details on these features consult the documentation supplied with your communications software Remote Programming all commands beginning with P can be used to program the incubator from the remote computer These commands are not case sensitive lower case or upper case letters w
50. ent f you have the 1 19 oxygen control option go back to Section 8 6 or if you have the 1 95 oxygen control option skip ahead to Section 8 8 8 7 1 Setting Up the N Tank Before you set up your oxygen control be sure you have the proper equipment for your nitrogen supply 2 cylinders of nitrogen regulation size W two stage pressure regulator Inline pressure regulator 6mm PVC tubing tubing clips Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 9 8 7 2 59 Set up the nitrogen tanks as follows 1 Inspect them to be sure there are no leaks or other damage 2 Check that the two stage pressure regulator valve and the inline pressure regulator valve are closed by trying to turn their knobs in a counter clockwise direction the knobs should be at the stop point Place the inline pressure regulator close to the incubator 4 Securely attach the two stage pressure regulator to the nitrogen tank s outlet and to the inline pressure regulator s inlet with tubing securing both ends with clips 5 Connect the inline pressure regulator s outlet to the incubator s N inlet on the right side of the equipment tray at the rear of the incubator see Figure 7 secure both ends with clips 6 Proceed as indicated in Section 8 7 2 NOTE To add an automatic gas cylinder changeover instrument with the incubator turned off connect its inlet to the two stage pressure regulator s outlet and the changeover
51. ependent on the ambient oxygen level A typical lifespan is 1 2 years at atmospheric levels During the sensor s lifespan the signal produced will slowly degrade until it is ultimately unusable For this reason we recommend that you reference the sensor to atmospheric oxygen levels on a monthly basis The OXYGEN SENSOR REF TO ATMOSPHERE procedure has three possible outcomes The first is that the procedure was completely successful and no further action need be taken until the following month s test The second and third outcomes are presented in detail in Sections 8 6 5 Replace Sensor Soon and 8 6 6 Replace Sensor Now See Section 8 6 7 for instructions on removing and replacing the sensor 8 6 5 Replace Sensor Soon If the referencing procedure was successful but the sensor is nearing the end of its working life the following message will appear in the display O2 REFERENCE OK BUT SENSOR REQUIRES REPLACEMENT SHORTLY PRESS ENTER TO PROCEED When you press the ENTER function key the message will change to this O2 SENSOR THE RESULT OF THE O REFERENCE PROCESS SHOWS THAT THE SIGNAL FROM THE O2 SENSOR HAS REDUCED INDICATING IT IS APPROACHING THE END OF ITS LIFE REPEAT THE REFERENCE PROCEDURE TO CONFIRM THIS RESULT PRESS ENTER TO PROCEED Press the ENTER function key 8 6 6 Replace Sensor Now If the referencing procedure failed Oxygen Control will be disabled but the incubator will function perfectly in all other ways unti
52. es which if not followed correctly could result in serious personal injury This particular Warning message represents a potential electric hazard WARNING This particular Warning message whether found in the manual or on the incubator means HOT SURFACE and therefore represents a potential danger to touch Crush Warning messages alert you to specific procedures or practices regarding heavy objects which if CRUSH not followed correctly could result in serious personal WARNING injury gt This symbol on the incubator is a reminder that it is of essential importance to read the user manual Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide P um 2 7 MS WARRANTY Every Instrument manufactured by New Brunswick Scientific NBS is warranted to be free from defects in material and workmanship This apparatus with the exception of glassware lamps and electrodes where supplied is warranted for 2 years against faulty components amp assembly and our obligation under this warranty is limited to repairing or replacing the instrument or part thereof which shall within 2 years following date of shipment prove to be defective after our examination Incubator accessories are warranted for 1 year This warranty does not extend to any NBS products which have been subjected to misuse neglect accident or improper installation or application nor shall it extend to products which have been repaire
53. he computer should be connected to a power supply outlet as close as possible to the incubator to avoid grounding problems Suitable communications software such as Terminal included with MS Windows 3 1 3 11 or HyperTerminal included with Windows NT 95 98 ME 2000 and XP The incubator requires the following settings in the communications software Baud Rate 19200 bits second Data Bits 8 Parity None Stop Bits 1 Flow Control Hardware some cables may require it to be None For details on using the RS232 interface see Section 8 4 New Brunswick Scientific User s Guide 4 ROUTINE MAINTENANCE 4 1 General Notes To ensure that chamber conditions remain as stable as possible be sure to minimize the length of time that the door is open When you open the door wipe off any small drops of condensate that may have formed on the inner seal This will avoid a build up of condensation If you are using the humidity tray for humidification be sure to follow the indications provided in Section 3 3 9 There is need to remove the top panel for normal maintenance or servicing so if you have two incubators stacked the upper incubator does not have to be moved when you are servicing the lower incubator 4 2 Daily Checks 1 Check that the temperature and CO levels are reading within specification 2 Check the reserve pressure in the CO cylinder normally 725 psi or 50 bar when full
54. igh temperatures involved serious burns could occur 0 The chamber walls and CO sensor all achieve temperatures of between 120 150 C particularly in areas that are inaccessible to chemical disinfection Certain areas of the glass door and inner door seal surface temperatures will be 5 of 120 C 6 After completion of the process one of the following status messages will be displayed If the cycle was completed successfully DISINFECTION COMPLETED OK is shown e was cancelled by the user DISINFECTION WAS ABORTED is shown e failed for any reason DISINFECTION FAILED CODE XX is shown The codes are listed in Table 7a and they are explained in Table 7b on the following page If this happens please note the failure code and contact your service representative for advice Table 7a Disinfection Failure Codes amp Descriptions Failure Code Failure Code Description see Table 5b below 01 2 02 03 2 04 X 05 X Z 06 W X 07 W X 2 08 Y 09 Y 2 0A W Y 0B W Y Z X 00 X 2 OE W X Y OF W X 2 New Brunswick Scientific User s Guide 50 Table 7b Disinfection Failure Code Explanations Failure Code Explanation Description Temperature drop during warm up period indicates the temperature fell more than 2 C during the heating phase over a 60 second period X Temperature drop during 4 hour pe
55. ile fully humidified liquid condenses around the sensor the result may be restriction of gas flow and a low sensor signal Should such condensation appear on the chamber walls normal operation can be easily restored by removing the humidity tray drying the chamber completely and then running the incubator at 37 C for one hour This will dry out the sensor After the hour has elapsed the humidity tray can be reinstalled and the incubator humidified again 8 8 Control 1 95 This oxygen control option uses the controlled addition of oxygen to increase the level above ambient and the controlled addition of nitrogen to reduce the oxygen level below ambient f you have the 1 19 oxygen control option go back to Section 8 6 or for the 0 1 19 oxygen control option go back to Section 8 7 N WARNING High oxygen levels inside the chamber require additional safety precautions Setting the oxygen to levels gt 24 can greatly increase the chance of fire and explosion Operators planning to set oxygen at 24 95 should therefore take all appropriate precautions to minimize the risk NBS does not recommend using external heat generating equipment inside the chamber when oxygen conditions will be gt 24 Before opening the incubator door make sure no flames Bunsen burner etc are in the vicinty Avoid the presence of other combustible gases hydrogen methane etc Ensure that all solenoid valves and pressure regulators
56. ket Figure 29 is located on the back wall of the chamber on the righthand side between the second and third shelf The socket is powered by an independent power inlet located above the equipment box at the rear of the incubator see Figure 28 below The socket also has an independent fuse which is rated at 4 Amps There is a dedicated switch for this socket illuminated green when it is on the switch is located on the left front of the incubator Figure 28 IP66 Enclosure C N gt l N m T i RI a 1 IP66 enclosure 3 IP66 power inlet 2 IP66 fuse holder 4 Equipment box CAUTION Working with electrical power inside a humid environment where the incubator is humidified can be hazardous The following precautions should be observed The instrument or equipment and its external connections to be used inside the chamber should be specified as suitable for use in a humid environment and at 37 C see also Using Powered Equipment within the Chamber If in doubt consult with the manufacturer of the equipment Always ensure the connections are properly and securely made Be sure to switch OFF the green illuminated switch on the front left of the incubator before connecting or disconnecting equipment inside the chamber continued Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 67 The Sealing Cap must a ways be in place when the
57. l a new sensor is installed and corrected references to the atmosphere The following message will appear in the display O2 REFERENCE FAILED PRESS ENTER TO PROCEED Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 55 When you press the ENTER function key the message will change to this O2 SENSOR THE RESULT OF THE O2 REFERENCE PROCESS SHOWS THAT THE SIGNAL FROM THE O2 SENSOR HAS REDUCED BELOW ACCEPTABLE LEVEL AND HAS REACHED THE END OF ITS LIFE REPEAT THE REFERENCE PROCEDURE TO CONFIRM THIS RESULT PRESS NEXT TO PROCEED When you press the NEXT function key the message will change to this O2 SENSOR OXYGEN CONTROL HAS BEEN DISABLED AS A RESULT BUT THE INCUBATOR IS OTHERWISE FULLY OPERATIONAL PRESS PREV TO VIEW PREVIOUS SCREEN PRESS EXIT TO EXIT When you press the EXIT function key you will return to the USER screen and normal operation 8 6 7 Removing amp Replacing O2 Sensor To remove and replace the oxygen sensor you will need the sensor removal tool provided see Figure 26 on the following page 1 Pull the rear access cover see Figure 25 off the rear outside wall of the incubator to gain access to the oxygen sensor Figure 25 Oxygen Sensor Rear Access Cover New Brunswick Scientific User s Guide 56 2 Reach inside and disconnect the sensor by unplugging the connector be sure to grasp the white connector body not the wire leads A WARNING Never pull on
58. l the incubator is level and stable Lock the leveling feet in place by tightening the locking nuts on each foot 5 Slide the humidity tray onto the lowest shelf rack supports which are shaped with stops to keep the tray in place see Figures 5 amp 6 New Brunswick Scientific User s Guide 16 Figure 5 Installing the Humidity Tray K 2 6 Making Connections Connect the CO gas supply with reference to Figure 7 on the following page 1 Connect the incubator to the supply using the 6mm plastic tubing with installed HEPA filter by attaching the tubing from the two stage regulator or in line regulator to the matching inlet on right side of the equipment tray 9 It is highly recommended that an in line regulator be used at the incubator s gas inlets In line regulators can also be mounted on the back of the incubator Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 17 2 Use the tubing clips provided to eliminate CO leaks N WARNING Slightly increased levels of may be found in and around the operating area of the incubator Over time this can have adverse effects on those exposed to such an environment Users working in environments with elevated levels of CO should take all appropriate precautions to protect their breathing
59. ly adjusted to 19 796 which is the true reading taking into account the relative humidity level Figure 23 Selecting OXYGEN SENSOR REF TO ATMOSPHERE EHTER 5 Enable the oxygen control a press the USER function key b using the A or V direction key select MANUAL DISABLE see Figure 24 c press the ENTER function key d select ENABLE for Oxygen Control using the 4 or P direction key then e press the ENTER function key Figure 24 USER SETTINGS Screen DISBBL FEES SENSOR FREF TO ATMOSPHERE IEMWARE UERSIONH EHTER Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 53 6 Navigate to the PROG screen to set the required Oxygen level 7 The Alarm levels are automatically set to 0 596 above or below the programmed value but you can modify them in the ALARM screen Re arming the alarm can be delayed until the programmed value is achieved select the relevant option in the ALARM screen 9 At low oxygen levels the and O levels may not have fully recovered within the Alarm limits after the 15 minute Delay in arming after door opening This time period can be increased in the ALARM screen to suit individual circumstances 8 By controlling the Duty Cycle of the valve Oxygen Control can be tailored to achieve programmed Oxygen and Carbon Dioxide levels at approximately the same time 8 6 3 Operating Guidelines
60. mperature is outside the specification of 0 1 C and each reading is shown as a single pixel When the temperature has settled within specification the recording is compressed to one pixel representing ten 18 second readings as long as the temperature remains in specification This allows up to ten hours of readings to be displayed on one screen When the temperature moves outside specification for instance if the door is opened the graph reverts to individual 18 second readings until temperature is within specification again When the data is compressed or decompressed a light dotted line is displayed vertically on the screen to signify that the time axis is changing from 18 second to 10 x 18 second increments or vice versa A heavy dotted line is displayed when the incubator is switched on New Brunswick Scientific User s Guide 28 3 7 3 3 7 4 3 7 5 3 7 6 Figure 15 TEMPERATURE GRAPH DOOR OPEN BAR CHART Screen Compressing data allows memory space to be maximized Once the memory space has been filled the earliest events are overwritten as they are replaced by the latest recording Graphical recording can be extended however to a number of years if your incubator is equipped with an RS 232 port by connecting the port to a PC loaded with GalaxyLog Software see Section 6 13 for more information CO GRAPH DOOR OPEN BAR CHART These graphs record in a way similar to the Chamber Temperature graphs The s
61. n 1 Use the A amp V direction keys to move the cursor Use the ENTER function key to select an option This section explains each of the USER screen features There are other USER options that may be displayed on this screen if they are installed on your incubator See Section 8 1 for a list of options 3 6 1 DATE TIME ADJUST The date and time is factory set and will only require adjustment if you are in a different time zone or when you change your clocks to Daylight Saving Time and back again to Standard time You may also select the style of display for the date A battery back up system ensures that the correct time date setting is never lost 3 6 2 AUDIO VOLUME ADJUST The audible volume can be adjusted to your own preferences New Brunswick Scientific User s Guide 24 3 6 3 PROGRAMMABLE CO AUTOZERO When you select this feature the PROGRAM CO AUTOZERO screen see Figure 12 below allows you to program the AutoZero frequency and time or to run the AutoZero function manually The AutoZero System automatically re references the Sensor to atmospheric CO in the following way 1 A pump activates for two minutes pumping HEPA filtered atmosphere at 0 3 liters minute into the sensor s measuring chamber This displaces the chamber atmosphere completely from the sensor Figure 12 PROGRAM CO AUTOZERO Screen SET RUTOZERO FREQUENCY SET PREFERRED TIME FOR A Z 07 00 11704 2002 OK RES
62. n AutoZero the CO level is too high Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 39 6 3 High Temperature Disinfection If your incubator is supplied with the High Temperature Disinfection option follow the guidelines outlined in Section 8 5 for correctly and safely operating this option New Brunswick Scientific User s Guide 40 7 SPECIFICATIONS Table 3 Specifications Galaxy 170 R Incubator Specifications Temperature Management see important NOTE on the following page Digital programming via microprocessor control in 0 1 C increments Measurement of chamber and door temperature via 6 RT matched thermistors sensitivity 0 01 C Adjustable independent control of door heater Out of Limits temperature protection system independent of microprocessor control Range 4 C above ambient temperature to 50 C Control 0 1 C Stability 0 1 C at 37 C Uniformity lt 0 3 C at ambient 20 25 C Recovery Rate Please refer to recovery graph If ambient temperature is close to the programmed value NOTE control settings may need adjusting Please consult NBS for instructions CO Control Solid state infrared CO sensor operating independent of humidity Programmable fully automatic zeroing function Range 0 2 2096 Control 0 1 Stability 0 296 at 5 CO Uniformity 0 196 Recovery Rate Please refer to recove
63. ner glass door options are available to match the shelves you choose to install which help to reduce the loss of temperature and humidity conditions when the chamber door is opened 8 13 Available Accessories Accessories NBS Part Number Two Stage CO Regulator P0628 5010 Two Stage N Regulator P0628 7220 CO Supply Line HEPA Filter P0628 5020 CO In line Pressure Regulator P0628 5030 CO Oylinder Auto Changeover Controller P0628 5000 Auto Zero HEPA Filter P0628 5060 CO Gas Analzyer Kit P0628 5040 Spare Gas Analyzer Tubes P0628 5050 Electronic Gas Analyzer P0628 6150 Electronic CO O gt Gas Analyzer P0628 6631 Electronic CO7 O Humidity Temp Analyzer P0628 6632 Additional Shelf Perforated P0628 6251 Additional Shelf Non Perforated P0628 6241 Additional Humidity Pan P0628 6260 Stacking Kit and Stand P0628 6270 Stacking Kit with Casotrs P0628 6490 Spare CO Sensor P0628 5700 Spare O Sensor P0628 5790 Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 69 9 CE CERTIFICATION New Brunswick aneppendorf company CE CONFORMITY CERTIFICATION DECLARATION OF CONFORMITY e RS Biotech Laboratory Equipment Limited 1 Drummond Crescent Riverside Business Park Irvine Ayrshire KA11 5AN Scotland UK Declare that the Galaxy 170 R CO incubator to which this declaration relates is in conformity to the following standards
64. ork exactly the same way The following are examples of how each of the above commands might be used PTnn n P rogram new T emperature setpoint Type PT37 5 then press Enter to reprogram the incubator s temperature setpoint to 37 5 C PCnn n P rogram new C O level Type PC04 0 then press Enter to reprogram the incubator s CO level to 496 PATHnn n P rogram new A larm T emperature H igh setpoint Type PATH38 0 then press Enter to reprogram the incubator s high temperature alarm setpoint to 38 0 C PATLnn n P rogram new A larm T emperature L ow setpoint Type PATL36 0 then press 1 Enter to reprogram the incubator s low temperature alarm setpoint to 36 0 C PACHnn n P rogram new A larm level H igh setpoint Type PACH05 5 then press 1 Enter to reprogram the incubator s high CO level alarm setpoint to 5 596 PACLnn n P rogram new A larm C O setpoint Type PACL04 5 then press Enter to reprogram the incubator s low CO level alarm setpoint to 4 5 In each case the change is confirmed by a message from the incubator e g Program Temperature 37 5 C OK or Program Alarm Temperature High 38 0 C OK etc Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 47 Status Commands all commands beginning with S can be used to interrogate the incubator from the remote computer and to display the current status of th
65. pecification for is 0 1 NOTE Both CO and temperature graphs share the same time axis If the time axis changes to accommodate data in one graph it will also change in the other DIAGNOSTIC CHAMBER ELEMENT GRAPH This graph records chamber element temperature over time to assist troubleshooting DIAGNOSTIC DOOR GRAPH This graph records the door s inner surface temperature over time to assist troubleshooting DIAGNOSTIC DOOR ELEMENT GRAPH This graph records door element temperature over time to assist troubleshooting Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 29 3 7 7 RESTART GRAPHIC RECORD This feature removes the current graph and begins a new one The data cannot be recovered once it is deleted 3 8 CHAMBER ALARMS Menu Screen The CHAMBER ALARMS programming screen Figure 16 allows the various alarm options to be selected and modified Press the A or V direction key to move around the options and the 4 or direction key to adjust values The temperature and CO2 High and Low Alarm setpoints automatically adjust to within 0 5 of the temperature and CO setpoints The alarm setpoints can also be manually adjusted Figure 16 CHAMBER ALARMS Screen To arm the Chamber Alarms after a selectable delay 1 Choose the option ARM ALARMS WHEN AT SETPOINT 2 Select NO for both TEMP and CO2 as shown in Figure 16 above 3 Choose the option DELAY IN ARMING AFTER DOOR OPEN and sele
66. rear of the incubator is not rotating when COOL or AMBIENT mode is engaged and there are no obvious obstructions contact your authorized service engineer for assistance If the temperature inside the chamber drops below 5 C while the cooling system is operating frost may form on the heat sink fins reducing airflow and adversely affecting performance Raise the temperature in the chamber to no less than 5 C to avoid frost Check that the rear fan inlet and outlets are not blocked and that there is sufficient clearance 2 inches 50mm between the rear of the incubator and the nearest possible obstruction such as furniture or a wall New Brunswick Scientific User s Guide 44 6 3 BMS Relay Contact Alarm Option The BMS Building Management System Relay Contact Alarm allows a signal from a central alarm system to be switched ON or OFF to indicate an alarm condition at the incubator The following alarms would activate the system over temperature under temperature system failure CO high and CO low As an integral option the alarm can be programmed to indicate when the power fails perhaps due to an electrical fault or is switched off If the power failure warning is active the relay contacts will be reversed pin 4 which is normally open becomes normally closed and pin 6 which is normally closed becomes normally open The alarm will also respond to other types of alarm depending on the options install
67. required the humidity tray should be filled with at least 1 5 liters but no more than 2 5 liters of warm 37 0 C distilled water at this time 9 The humidity tray should always be left place even if the incubator is not being humidified It also serves as a spill tray For cell culture work we recommend the use of copper sulphate or a recognized biocide in the humidity tray Tests have shown that in addition to inhibiting bacterial growth in the tray this can reduce contamination on the chamber walls Add one small teaspoonful 0 5g of copper sulphate to the water in the humidity tray For IVF and other sensitive work we do not recommend the use of any biocide in the humidity tray To reduce the possibility of contamination every 10 to 14 days empty the tray clean it with a solution of 70 isopropyl alcohol and 30 distilled water and then refill it with 1 5 liters of warm distilled water CAUTION To avoid possible damage to the sensor never leave water in the humidity tray while the incubator is switched off or when a high temperature disinfection cycle is initiated if you have this option The humidity level within the chamber is not adjustable The internal chamber will reach 95 relative humidity at 37 C using the 1 5 liter humidity tray 3 4 Programming 3 4 4 Temperature and CO Level To set the desired operating temperature and level Press the PROG function key see Figure
68. riod indicates the temperature fell below 118 0 C during the disinfection phase Y Temperature increase during cool down phase indicates the temperature rose by more than 2 C during the cooling phase over a 60 second period 2 Cancel key pressed 0 If the incubator power is cycled OFF then ON during a disinfection cycle due to a power outage the incubator will power up as normal This condition will be indicated by the absence of a completed disinfection status message DISINFECTION COMPLETED OK If the chamber temperature is above the setpoint or the element temperature is greater than a factory preset control point cooldown will be entered until these conditions are satisfied 9 It is recommended that the AutoZero function be run following each disinfection cycle 8 5 2 High Temperature Disinfection Option with Oxygen Control The Oxygen Sensor is an electrochemical device that will be destroyed by the high temperature used to disinfect the incubator if left in place For this reason the Oxygen Sensor must be removed from the incubator prior to a High Temperature Disinfection Cycle The sensor can be accessed from the rear panel of the incubator Detailed removal and installation instructions are provided in Section 8 6 7 CAUTION The incubator should be positioned to allow access to the back of the incubator for removal of the Oxygen Sensor prior to starting a High Temperature
69. rt O sensor location not shown here 5 Sensor cover holder Location for mounting in line regulators 6 Removable lifting handles 2 Ensure that the white porous sensor cover remains in place 3 Using the power cord provided connect the incubator to a grounded power supply 4 Switch the incubator ON using the on off switch at the rear of the cabinet The display will illuminate immediately 5 Turn on the CO gas supply with the pressure regulator set to 5 psi or 0 35 bar 6 The chamber setpoints are pre programmed at 37 0 C and 5 Leave the incubator on until the programmed chamber temperature and concentration have been reached 0 NOTE The incubator s CO valve is disabled until the incubator reaches the temperature setpoint After the temperature setpoint is reached the CO valve is activated allowing the incubator to reach the CO setpoint If power is interrupted to the incubator long enough for the temperature to drop below setpoint the CO valve will be deactivated until temperature setpoint is again achieved This serves to avoid spurious readings while the incubator is below its temperature setpoint Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 21 7 Leave the incubator running for at least two hours preferably overnight to allow conditions to stabilize 3 8 Using the Humidity Tray amp Humidity Control If humidification is
70. ry graph Required Gas Pressure Gas Connections 6mm tubing 5 psi 0 35 bar Relative Humidity Removable stainless steel humidity tray Reservoir Capacity 2 5 liters Humidity Control Normal 9596 at 37 C Shelves Polished stainless steel perforated standard Dimensions WxD 520 x 426mm 20 5 x 16 8 inches Number of Shelves 4 standard up to 8 shelves with multiple position option Alarm Systems Two level alarm system giving programmable audio visual warnings with options for remote communication Level 1 signals system failures level 2 is programmable and monitors chamber conditions Dimensions Chamber HxWxD 693 x 540 x 444mm 27 3 x 21 3 x 17 5 inches Chamber Volume 174 liters External HxWxD 848 x 685 x 677mm 33 4 x 27 x 26 7 inches External Crated HxWxD 1100 x 830 x 830mm 43 x 33 x 33 inches pallet included RT Resistance Temperature curve Galaxy R Series 170 CO Incubator CO170R 0050 User s Guide 41 Galaxy 170 R Incubator Specifications Weight Crated 115kg 253 5 Ibs Uncrated 90kg 198 4 lbs with standard features Grounded Electrical Supply Voltage Power 100V 120V 50 60 Hz 1300 W 220V 240V 50 60 Hz 1600 W Energy to maintain 37 C lt 0 1 kWh Storage Temperature 10 50 C 120V 2
71. s estosen ossee Seneste 70 10 2 jUOnTr 8 70 11 INDEX e M 71 Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 1 INTRODUCTION The Galaxy R Series CO incubator model 170 R is microprocessor controlled and designed to ensure accurate and reliable operation The incubator incorporates a large back lit LCD display touch sensitive keypad and sophisticated control system that allows for easy programming control and monitoring of the chamber conditions The display also provides for graphical viewing of system data on screen messaging and help files to simplify operation and maintenance A direct heating system utilising a thermal heating element completely surrounds the incubator providing an even temperature within the chamber The independently and directly heated outer door is designed to ensure an even distribution of heat This system ensures a rapid controlled return to optimum chamber conditions after a door opening while also preventing any overshoot The incubator s direct heat system provides for optimal use of laboratory space by allowing the most efficient internal volume for the footprint of the instrument A solid state infrared sensor is used to control the level of providing excellent reliability and remaining unaffected by humidity
72. sensor s and may affect your warranty 5 Youcan clean the humidity tray by rinsing it in sterile water wiping it down with the alcohol solution and then rinsing it again with sterile water 6 Wipe down the inside of the chamber with the alcohol water solution and leave it to dry completely CAUTION Never use any of the following substances to clean the stainless steel or damage will result Sodium Azide Aqua Regia lodine Ferric Chloride or Sulphuric Acid 7 Wipe the internal components of the chamber twice with the alcohol water solution Wipe off excess liquid and leave it to dry completely 8 Reassemble the shelf racks shelves and humidity tray before switching the incubator on Wipe the inner door seal with the alcohol solution rinse and leave it to dry 9 Ensure the protective cover s are removed from all sensor s and replaced in the holder for safekeeping Be very careful as you remove the black CO sensor cover not to accidentally remove the white porous sensor cover This must remain in place 10 Refill the humidity tray as explained in Section 3 3 When you reinstall it ensure that the humidity tray is pushed fully back 11 Leave the incubator on for at least two hours preferably overnight to allow conditions to stabilize 12 When the incubator has stabilized carry out an AutoZero and reprogram the desired CO level It may be necessary to open the glass door briefly if after performing a
73. snsnssesnensnsenenene 32 RS232 Interface 33 ROUTINE MAINTENANCE eene nennen nennen nennen nennt nnne innen nn 34 jUnnnn S 34 DETINA Y C 34 I2 A ABL IO CH 34 Monthly Check HR 34 Sampling with Analyzer ssossossssnssnsnsnssnssnssnsnssnsnnsnnsnssnsnnsnnsnsnnsnnsnsnnsnnsnsnnsnnsnnsnssnsnnnsunsnnsnnnnen 35 Routine Checks of the Control Option sursssossnsnsnssnssnsnnsnsnsnnsnssnsnnsnnsnnsnsnnnnsnsnnsnnsnsnnsnnsnnunsnnen 35 SERVIGB E T A siiis aiia rcd Gad Cl E ar DER UE RI ap Gor eris E vex E CUM AE 36 Fuse soise ssois 36 CLEANING amp DISINFECTING unscunaunnsnann oko ons eee dn ediscere 37 Grip 37 Disinfecting usossossssssnssnsnnsnssnssnsnssnssnsnnsnssnsnnsnssnsnnsnnnssnsnnsnnsnsnnsnnsnnsnssnsnsnssnsnnsnsnsnnsnssnssnsnnsnssnsnnsnnsnnnnn 37 High Temperature Disinfection orsussussssnssnsnnsnssnsnnsnssnsnnsnnsnnsnsnnsnnsnssnsnnsnssnsnnsnnnsnnsnnnnsnsnnsnnsnnensnnen 39 ore cR b Ee 40 OPTIONS amp 42 Available Options RR 42 Cooling S
74. such as a heater an autoclave or an oven e Near the exhaust of heat or cold generating equipment like an ultra low temperature freezer Near a window exposed to direct sunlight e Directly on top of any heat generating apparatus e Without minimum ventilation clearance of 0 5 inch 10mm all around 2 inches 50mm in back if you have the cooling system option CAUTION NEVER try to lift the incubator by its door this would cause permanent damage to the incubator NEVER lean on or place objects on the open door Remove the incubator from the pallet as described in Section 2 2 and place the incubator in the working position on a level surface capable of bearing its weight approximately 200 15 90 kg actual in use weight will naturally be heavier and will depend on both the options installed and the material stored in the incubator The incubator is designed so that one incubator can be safely stacked on top of either another Galaxy 170 R or a Galaxy 170 S using the stacking kit which includes instructions It is not possible to put any other type of incubator or heavy apparatus on top as the top cover and stacking kit are designed to support only the feet of another Galaxy 170 incubator 9 When the incubator is securely place remove the four lifting handles Store them in a safe place for future use 2 5 Setting Up Place the anti slip covers provided onto the incubator s front feet keep them installed at all
75. the display flashes the audible alarm sounds and a message appears on the screen see Figure 17 You can acknowledge the alarm by pressing any key Figure 17 CHAMBER ALARM Message PROGRAMMED TO Sre UFL 5 02002 md H I eH ALARM After setpoints have been achieved for the first time when the inner door is opened the alarm system is disabled on closing the door if selected a programmable alarm delay starts If chamber conditions recover within the programmed alarm delay time the alarm system is immediately re armed After the delay the alarm system is armed and if the temperature and CO are outside the alarm high amp low settings the alarm will be activated Ifan alarm occurs and the chamber subsequently recovers the alarm stops and the system is re armed Details of the alarm event are stored in the datalogger Figure 18 on the following page is a flow chart representing the chamber alarm system Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide Figure 18 Chamber Alarm System Flow Chart v ARM ALARMS lt IS TEMPERATURE OR CO2 LEVEL MORE THAN 0 5 OUTSIDE PROGRAMMED POINTS o CHANGE OPEN INNER DOOR SWITCH ON PROGRAMMED VALUES Y DISARM ALARMS CLOSE INNER DOOR igi gt ARE TEMPERATURES AND CO2 LEVELS WITHIN 0
76. the wire leads disconnect the white connector body 3 Using the sensor removal tool see Figure 26 unscrew the oxygen sensor by turning it counter clockwise Because the oxygen sensor contains lead be sure to dispose of it according to local regulations Figure 26 Oxygen Sensor Removal Tool 4 Using the sensor removal tool install the new oxygen sensor by turning it clockwise until it is finger tight Do not use excessive force or any metal tool 5 Reconnect the sensor by plugging the white connector body back in 6 Make sure that the sensor wires are inside the metal sensor tube to protect them from damage then press the rear access cover snugly back in place 7 After replacing the sensor humidify the incubator and allow it to stabilize overnight 8 Calibrate the sensor with reference to the atmospheric oxygen level see Section 8 6 4 8 6 8 Replacing the Filter Disc The hydrophobic filter installed on your Oxygen Control system helps prevent condensation from reaching the sensor see Figure 27 To replace the hydrophobic filter disc membrane with reference to Figure 27 1 Carefully pull the complete hydrophobic filter holder away from the oxygen sensor holder on the rear wall of the chamber Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 2 57 With a fingertip or a 10 1 1mm inch rod from the rear of the filter holder gently push the filter membrane disc and the filter c
77. times New Brunswick Scientific User s Guide 14 Install the wire shelf racks and shelves and level the incubator 1 Oriented as shown in Figure 1 place the shelf racks inside the chamber Each rack has three rubber feet two stand on the floor of the chamber and one stands against the back wall Make sure the cushioned tubing spacers are snug against the side walls these spacers allow clearance for the shelves Figure 1 Inserting Shelf Racks 9 Only shelf racks for 4 shelves are shown this manual Shelf racks for 8 shelves are also available 2 Install the tie rod at the back of the shelves to hold both sides together as shown in Figures 2 amp 3 on the following page You may prefer to do this prior to inserting the racks in the chamber Figure 2 Fitting Tie Bar to Shelf Racks 2 1 Tie Bar 2 Detail 2 3 scale shows the tie bar s anti tip groove Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 15 Figure 3 Shelf Rack Assembly T Y Gf os mu m t nz 3 Install the shelves see Figure 4 beginning at the top Make sure that each shelf s anti tip groove see Figure 2 detail is properly inserted onto each of the shelf rack guides Figure 4 Inserting Shelves 4 Level the incubator by adjusting the feet Place a small level on the second shelf of the incubator Adjust the leveling feet unti
78. uch or handle the white CO sensor cover be sure to wear gloves and do not later touch those gloves to your face Discard or wash the gloves 2 3 Utilities In order to use the incubator you will need Table 2 Utilities Utility Requirement Electricity 100 120V 50 60 Hz grounded electrical supply with minimum capacity of 10 amps OR 220 240V 50 Hz grounded electrical supply with minimum capacity of 8 amps CO gas Cylinder with 10096 CO vapor withdrawal together with a two stage regulator for pressure control to 5 psi or 0 35 bar see WARNING below WARNING For proper incubator operation gas pressure must not exceed 5 psi Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 13 2 4 Location The incubator is designed to operate at a chamber temperature of 4 0 C above ambient and at an absolute minimum ambient temperature of 15 C if the incubator is being used at 37 C Maximum allowable ambient temperature is 35 C 9 Position the incubator allowing enough space to open the door and to access the CO sample port on the left side of the incubator Care should be taken to avoid placing the incubator in a position that may affect its performance such as those listed below DO NOT place the incubator e Directly under beside or within the air flow of heating or air conditioning ducts or other drafts e Directly beside heat generating equipment
79. ure regulator 9 the programmed O level is close to the ambient oxygen it may be necessary to reduce the cylinder pressure below 1 bar to stop the oxygen level from undershooting the programmed value Be sure to humidify the incubator and leave it overnight to stabilize before proceeding further To automatically calibrate the sensor to atmospheric oxygen levels select OXYGEN SENSOR REF TO ATMOSPHERE in the USER menu and then follow the onscreen instructions The oxygen reading is automatically adjusted to 19 7 which is the true reading taking into account the Relative Humidity in the chamber 8 8 3 Enabling or Disabling O2 Control To enable oxygen control 1 Select the USER screen by pressing the USER function key 2 Use the direction keys to select MANUAL DISABLE 3 Press the ENTER function key then use the direction keys to select ON for Oxygen Control 4 Press the ENTER function key again Program the required oxygen level in the PROG screen following the onscreen instructions To disable oxygen control follow the pattern used to enable it but this time select USER MANUAL DISABLE ENTER OFF and then ENTER again New Brunswick Scientific User s Guide 64 8 8 4 Important Notes for O2 Levels gt 80 If you are running the incubator with a dry chamber and no the recommended maximum O2 level is 9596 In addition open samples inside the chamber can cause RH to increase and the O2
80. wing page displays the following information New Brunswick Scientific User s Guide 26 Figure 13 DATALOGGER Screen This section explains these features 3 7 1 ALARM EVENTS The following alarm events are recorded in the order in which they occurred with the most recent event displayed at the top Power ON OFF Chamber Temperature High Low programmed value Level High Low programmed value Supply Failure System Alarms CO Auto Zero A Z Adjustments Auto Gain A G Adjustments reserved for use by authorized service technicians only Oxygen and Relative Humidity R H Alarms where these options are installed The capacity is 99 events after which the earliest event is overwritten and a later event is added The date and the time are also recorded for each event as shown in Figure 14 on the following page Galaxy R Series 170 R CO Incubator CO170R 0050 User s Guide 27 Figure 14 ALARM EVENTS Screen 1 1 1 1 1 1 1 1 1 1 1 1 1 To scroll the screen back press the W direction key Press A to go back to the first screen 3 7 2 TEMPERATURE GRAPH DOOR OPEN BAR CHART When you select this from the DATALOGGER screen the Door Open bar chart is shown at the top of the screen to associate it with a temperature disturbance note the correlation in Figure 15 on the following page A temperature reading is recorded every 18 seconds while the te
81. with the optional cooling system see Section 8 2 Factory testing can be carried out with the specific apparatus to ensure that the control conditions can be met To connect to the socket see Figure 29 on the following page inside the chamber a power cable with a matching IP66 plug is supplied This power cable should be fitted by a qualified person to the equipment or device to be used inside the chamber If the cable cannot be fitted contact your distributor giving details of the connectors required so they can supply a suitable lead CAUTION The Electrical Socket and the matching plug are both sealed to IP66 and are capable of being heated to 120 C during the High Temperature Disinfection cycle but the electrical cable is NOT rated for use at 120 C Therefore the equipment or device and the connecting cable should be removed from the chamber and the Sealing Cap replaced on the socket prior to starting a High Temperature Disinfection cycle New Brunswick Scientific User s Guide 68 8 11 Figure 29 IP66 Socket AOUN Copper Inner Chamber Pin Connections Live Neutral Ground Not in use at this time Copper naturally changes color This incubator had a bright finish when it was manufactured The dull finish that you see is a result of oxidation of the copper surface itis the properties of this oxidation that create the important anti microbial surface 8 12 Other Options Four and eight in
82. y 5 Repeat steps 3 and 4 every 30 minutes or so keeping an eye on the DATALOGGER Graph The oxygen level should rather suddenly recover after a few hours 6 Leave the incubator for a few more hours to be sure that the sensor membrane has thoroughly dried out 7 Replace the hydrophobic filter disc following the instructions in Section 8 6 8 8 Re humidify the incubator 9 After 2 or 3 hours carry out an OXYGEN SENSOR REF TO ATMOSPHERE procedure When the referencing has been successfully completed the incubator is ready for use DO 8 6 10 Specifications The Oxygen Control option has the following characteristics Sensor Type Self powered diffusion limited electrochemical cell with temperature compensation Zero Signal in Nitrogen lt 50 Temperature Compensation 2 of signal variation from 0 40 C Relative Humidity Range 0 99 non condensing Operating Temperature Range 20 C to 50 C Resolution 0 0196 Oxygen Expected Operating Life 1 2 years in ambient oxygen Hydrophobic Filter Operating No data available on the filter lifespan but we are Life confident to expect it will last at least 6 months Nitrogen Input Rate 20 l min at 1 bar 14 5 psi Typical Oxygen Reduction 3 minutes to 1696 Rates 4 minutes to 1196 8 minutes to 6 8 7 OsControl 0 1 19 This oxygen control option uses the controlled addition of nitrogen to reduce the oxygen level below ambi
83. ystem Option Troubleshooting the Cooling System BMS Relay Contact Alarm Option usssssssassnssssossnsnnsnssnsnnsnnsnssnsnnsnnsnssnsnnsnssnsnnsnnsnsnnsnnsnssnsnnsnnsnnunsnnen 44 jL 45 High Temperature Disinfection orsassussssnssnsnnsnrsnsnnsnssnsnnsnnsnnsnssnsnnsnssnsnnsnssnsnnsnnsnssnsnnnnnsnsnnnnsnnensnnen 48 Using High Temperature Disinfection 48 High Temperature Disinfection Option with Oxygen Control sss 50 Control 1 19 sassesnsnenesnsnenennsnenesnensnnsnsnenennenenssnsnensnnsnsnssnsnenssnensnnsnsnenssnenssnsnenesnensnssnsnssnsnensnnsnssene 51 Setting Up the No Tank eiecit EORR REO TOR RD RD ran 51 setting Up Oxygen Control irt eie an e n n e E D e e ERU re eranl 51 Operating Guidelines an ter rer P RYE ER EP EMEN RR P CE aeter oie 53 Referencing to Atmospliere i etae tton ee o HIR E ERE 54 Replace Sensor Soon ensure 54 Replace Sensor NOW eite giten e ei oce deest equites 54 Removing amp Replacing Sensor sse nennen 55 New Brunswick Scientific User s Guide 8 6 8 Replacing the Filter DISC es ree ed e a eH e Orte rni ex 8 6 9 Troubleshooting the Oxygen Sensor 8 6 10 Specifica ons E 8 7 OUnP 87 Setting Up the N5 Tank terror tdt terrier torri Den e e Mee PE v 8 7 2 Setting Up 8 7 3

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