Manual CAR_Ing V 4 Jul 09
Contents
1. Fig 42 Manual grid alignment screen Clinical Array reader CAR Manual Version 4 July 2009 39 Clinical Array Reader CAR User Manual GEN MICA ee x Webs Zooms no L E Zoom Zoom Zoom Reanalyze Reanalyze Cancel 2e yA 4 Fig 43 Aligning the metal markers on the reanalysis menu Zoom tool placed on the upper right corner of the screen facilitates the reanalysis to user Fig 44 VEE Zoom Fig 44 Manual grid alignment in strips using metal markers and zoom tool Once the metal markers are aligned press Reanalyse option Fig 44 and a image with the grid will show the final alignment If both dots and metal markers are well aligned then press Accepf lower right side If new adjustments are required press Retry Fig 45 Clinical Array reader CAR Manual Version 4 July 2009 40 Clinical Array Reader CAR User Manual GEN MICA fMo ololololololo ole OO oloo O O O O 6 6 O O o O O o O O O 6 G O 6 E O 6 e 6 o elelel e o e eBMoooccooco fe Zoom cept Fig 45 Grid alignment checking au Manual grid alignment implies user s intervention in the r2. g EE _ A I I l J aiin aiin T a q j X a HEE EEE EE amp amp Fig 7a Selecting Edit option on the New Analysis menu Selected column 4 Select Assay CLART_HP 2_MM v CLART_HP 2_MM CLART_HPV2 O Barcode Reader Pneumovir a amp eded EJES C le Ca L s ol LJ LJ Lessee i Fig 7b Manual setting of Assays IDs A pull down menu shows the multiple choices to choose among Clinical Array reader CAR Manual Version 4 July 2009 17 Clinical Array Reader CAR User Manual GEN MICA Press OK when finish to return to previous menu and follow up with the visualization process Once assigned the assays IDs strips will turn to the different colour combination specific for each type of analysis Fig 7C Select Rack 96 Well Plate x APRS Ra E ie 4 oma amp T _ h d d E bza ho TELLI Lae b i A ko a i d n h D mm EN ta E pm yh An y 80eeo00e 1 A a 4 Nae Ta Set Assay IDs Set Sample IDs Fig 7c Diferent colours combinations corresponding to different assays 8 3 SETTING SAMPLES IDs In the same way that in previous sections setting Samples IDs can be carried out automatically or manually In case of no Samples IDs assigment software will set automatically a ch correlative number to each sample from 1 to 96 starting with the sample placed in position 1A upper left
3. Setting Samples IDs Automaticall cy Barcode reader is required to set Samples IDs automatically and barcodes must be set and printed before start setting the IDs Set and print barcodes before starting Clinical Array reader CAR Manual Version 4 July 2009 18 Clinical Array Reader CAR User Manual GEN MICA Press Set Samples IDs option and read the previously assigned barcodes normally The software associates automatically a barcode to a sample position Samples with already assigned ID will appear in black Fig 8 Select Rack Set Manually o ooo a o a a ao a o m Ea Set Assay IDs Stop Import Cancel Next gt gt Fig 8 Setting Samples ID automatically Once finished importing IDs press Stop Import to return to the New Analysis menu Setting Samples IDs Manually Select each position individually by pressing twice on it A new window representing a tactile key board will pop up on the screen Fig 9 Use it to set samples ID manually Selected Strip Position A1 Samples ID 98987 Cancel OK Be a 0 ed HHHOH ISIE IASG AGC SPACE BAR 7 Copy Paste Y B N M CLEAR SHIFT Fig 9 Setting Samples IDs manually using the tactile keyboard Clinical Array reader CAR Manual Version 4 July 2009 19 Clinical Array Reader CAR User Manual GEN MICA The user can check the sample position at an
4. fem a S d q r b d E F oY OOOO be od DR amp S amp S a Mm d gt lt a g P 5 LAA A b p A A ji d ii an TS hah a A YY Y z i A i A e ai D ai L11 Y aa A d es Ve _ aS a www 7 I IS T j J g T Se aS aS a lana ae 4 Y Y Y WY w d IEA Cae A w i d d a q ye eT A Ge gt Gh Be amp LA AA A A A ae E d 5 Y Y Y Y Y A A arg a Si d 4 L b h gt ie E j m eee ie eae ena Edit Edit Edit Edit Edit Edil Edit Edi Edit Edit Edit Edit aE Set Assay IDs Set Sample IDs Cancel Next Stop Import Set Sample IDs Cancel Next A Fig 4 Left Set Assay ID option on the lower left side of the screen Right All strips turn unselected when chosen this option When reading a barcode an AS is activated automatically on the microplate sketch and colour combination turns from grey into a specific assay s colour combination Fig 5 Clinical Array reader CAR Manual Version 4 July 2009 15 Clinical Array Reader CAR User Manual GEN MICA Select Rack 96 VVell Pla TATTI dit Edit dit dit dit Edit Edit Edit Edit Edit Edit Stop Import Set Sample IDs Cancel Next gt gt Fig 5 Specific colours combinations Red HPV Blue Pneumovir Green Entherpex Manual setting Software s default settings establish the analysis of the whole microplate composed by up to 12 ASs All ASs not includ
5. menu following the next protocol New Analysis Service Select Archive option from the Main Menu GENS MICA Archive Shut Down Clinical Array reader CAR 1 July 2009 55 Clinical Array Reader CAR User Manual GEN MICA Fig 74 Once selected this option a searching engine will pop up On this new window it s possible to restrict the searching time period inserting a start searching date and end searching date Select Start End Date Start Date 2007 09 15 End Date Year Month Day lt lt Back Next gt gt Fig 75 Search engine will find out all results contained between the two indicated dates In order to specify the search three extra searching parameters are available Sample ID Search for samples with same code or ID analyzed during the specified time period Assay ID Search samples processed with same procedure or assay during the specified time None Search all samples included in the indicated time period Clinical Array reader CAR Manual Version 4 July 2009 56 Clinical Array Reader CAR User Manual GEN MICA Select Filters Sample ID 5647898 o Assay Name 40204 None lt lt Back Next gt gt O88 eo Jee Seog gee eee AHAHAH ee ce Fig 76 Press Next to continue 11 3 BARCODE READER CHARACTERISTICS AND INSTALLATION A simple barcode reader will be advisable since insta
6. CAR and then turn off the main switcher at the back side lt is important to shut down the CAR when not required as this may lengthen its half life Fig 1 Clinical Array reader CAR Manual Version 4 July 2009 12 Clinical Array Reader CAR User Manual GEN MICA New Analysis Service GEN MICA Archive Shut Down Fig 1 Shut down option at the GENOMICA S main menu Do not switch off the reader directly since it could damage tt Clinical Array reader CAR Manual Version 4 July 2009 13 Clinical Array Reader CAR User Manual GEN MICA 8 8 PERFORMING STRIPS ASs Reading and analyzing samples is a sequential easy procedure compose by multiple menus where user can choose among the several options of the application For starting a new analysis choose option New Analysis on the application s main menu Fig 2 GEN MICA Archive Shut Down Fig 2 Main menu of the GENOMICA s application Once this option is selected a new menu will pop up on the screen where 96 wells microplate sketch is depicted Fig 3 Select Rack 96 well plate o o jo o o o o o o o o a Sam ER z A koo t Hi a i q FY A b d pa cL ae lt bd h a TI rN d amp Y J E d ay a yy S g a z E a i i ON as ey aN E i 3 r Y YF L k N h a d E FEEEE Set Assay IDs Set Sample IDs Fig 3 96 wells microplat
7. FAQ Image To visualize the image obtained by the CAR Fig 18 This image can be exported or printed individually with print option Raw Data To consult raw and normalized numerical values obtained in the analysis of the samples Type 6 ype 11 ype 16 ype 18 ype 26 ype 31 Type 33 ype 35 Type 39 ype 40 ype 42 ype 43 Type 44 ype 45 ype 51 Tima 9 Virus MaAnatiin Dannan lt lt Back Fig 16 Results menu Export Image Raw Data By selecting the Image option user accesses to the image corresponding to the analyzed sample Fig 17 and 18 Clinical Array reader CAR Manual Version 4 July 2009 37 Clinical Array Reader CAR User Manual GEN MICA Virus Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative j Negative Passed Type 45 Negative Passed Type 51 Negative Passed Tuna FA Manntivn Darena Fig 17 Selecting Image option Fig 18 Example of a HPV assay performed in strips metal markers By selecting Raw Data option user accesses to the numerical values measured for each virus type Fig 19 and 20 Virus Virus Resut Controls Expo Type 6 Type 11 z Print Type 16 Negative Passed Type 18 Negative Passed Type 26 Negative Passed Image Type 31 5 1 0000 Type 33 Nega
8. marked in yellow Insert the safety lock screws recently removed green circles below in the positions marked in red See below 3 Once installed screen s arm should appears as follow Clinical Array reader CAR Manual Version 4 July 2009 9 Clinical Array Reader CAR User Manual GEN MICA 4 4 CONNECTING SCREEN AND READER Clinical Array reader CAR Manual Version 4 July 2009 10 Clinical Array Reader CAR User Manual GEN MICA 1 Connecting cables 1a Connect the power supply Type Jack 3 5 1b Connect the USB connection to one of those at the right side 1c Connect the VGA connection to the blue connector 2 Switch on the screen by pressing the button on top 5 SAFETY NOTES Please be particularly aware of electrical hazards Standard electrical safety precautions must be applied at all times Only qualified personnel are permitted to perform electrical servicing Never touch switchers or outlets with wet hands Always switch off the instrument before disconnecting the AC power cord Always unplug the instrument prior to cleaning major liquid spills and prior to servicing the electrical or internal components Do not open the instrument case Avoid any movement or vibration during reading operation 6 PROCEDURAL LIMITATIONS Clinical Array Reader CAR was designed for the detection and analysis of ASs Array Strips or ATs Array Tubes only Any other tubes vials or ma
9. them are in the main directory e g D and not included inside any folder Removable Disk D bis im File Edit wiew Favorites Tools Help Search ear Folders Eee Address S Di T Go D fel fel fi patch HPV2_ instal Mebtaboneli Preurnovir_ins 64 8 MB i My Computer Fig 63 Connect your pen drive to a free USB connection available on the back of the reader Clinical Array reader CAR Manual Version 4 July 2009 50 Clinical Array Reader CAR User Manual GEN MICA Fig 64 Go to the Service menu New Analysis GEN MICA Fig 65 Selecting Service option Press Import button Clinical Array reader CAR Manual Version 4 July 2009 51 Clinical Array Reader CAR User Manual GEN MICA System 0 ooog Backup Reader Menu Fig 66 In the dialog box you should see both drives C and D Corresponding to the USB stick In some cases it takes a couple of minutes to the reader to recognize the pen drive In those cases go back to the principal menu and try again in a few seconds CA i DA Fig 67 Browse the pen drive contents by pressing on the plus symbol You can see there the patch folder and the kits installation programs in blue Clinical Array reader CAR Manual Version 4 July 2009 52 Clinical Array Reader CAR User Manual GEN MICA C patch HPV2_install bat Metabone_install at Pneumo
10. 0003 O 40 Fig 40 Reading process 9 8 SUMMARY REPORT AND RESULTS SUMMARY REPORT The CAR generates a final report including following information Date and time Position of the sample Assay ID Sample ID Results for each sample User can look up the final report by selecting Report View option From this menu it is also possible to print it out in PDF format Fig 14 Working list GenomicaSAU Rev 14 CAR CAP System Run ID 190003 Date and time Wed Feb 25 09 54 28 2009 GEN MICA Pay amy 7 Sample sSrence 40207 LART HPV2 F 000000040207 402 ART HPV2 oR PEST A2 40207 CLART HPV2 MM 2000000 LART HPV2 MM z i Operational Mode Process and Reading Reading Fig 14 Summary report created by the CAR at the end of the analyzing process Clinical Array reader CAR Manual Version 4 July 2009 36 Clinical Array Reader CAR RESULTS User Manual GEN MICA To visualize a specific sample s result select the sample position on the microplate sketch screen Fig 15 by pressing twice on it A new menu showing working list and results will appear on the screen Fig 16 On this menu following options are shown Export To export reports and results to an external server LIMS or USB Print If an external printer is connected via USB port the selected result s page or all results data pages including test image can be printed by operating the Print button See
11. 0207 i 4 B2 40207 CLART HPV2 MM 000000090207 3 C2 40207 CLART HPV2 MM 00000040207 6 Lv Operational Mode Process and Reading Reading Fig 37 Workinglist when performing ATs 9 7 READING PROCESS Reader s tray opening and closing processes are automatic Do not try to force A or move it manually If neccessary press the emergency button placed on the rear side of the CAR User will be requested to initialize the process Fig 38 by pressing the Open Tray box Clinical Array reader CAR Manual Version 4 July 2009 34 Clinical Array Reader CAR User Manual GEN MICA GEN MICA Open Tray Fig 38 Message to initialize the reading process Once finished the opening process place the tubes adaptor ATs manually in the tray and operate the Close Tray box Fig 39 GEN MICA Close Tray Fig 39 Message to activate the closing process of reader s tray Reading procedure can be monitored in the screen since samples wells change their colours indicating the reading status Fig 40 Wells in green Sample is already analyzed Grid alignment succeded Wells in red Sample may be reanalyzed Grid alignment failed see Sample Reanalyze section below Wells in yellow Sample has not been analyzed yet Clinical Array reader CAR Manual Version 4 July 2009 35 Clinical Array Reader CAR User Manual GEN MICA Run Number 1442 Plate ID 10
12. Clinical Array Reader CAR User Manual GEN MICA CUINICAU ARRAY READER CAR USER MANUAL Clinical Array reader CAR Manual Version 4 July 2009 1 Clinical Array Reader CAR User Manual GEN MICA 1 TABLE OF CONTENTS 1 Table of contents 1 2 Introduction 4 3 Components Description and Specifications 5 4 Installation 7 4 1 Precautions 4 2 Unlock ILock 4 3 Assembling Screen 4 4 Connecting screen and reader 5 Safety Notes 11 6 Procedural Limitations 11 7 Switching on off 12 8 Performing Strips ASs 8 1 Setting Samples Number 14 8 2 Setting Assay IDs 17 8 3 Setting Sample IDs 18 8 4 Working List 20 8 5 Reading 20 8 6 Summary Report and Results 22 8 7 Sample Reanalysis 25 9 Performing ATs 9 1 Placing ATs on the Adaptor 28 9 2 Changing Rack Type 29 9 3 Setting Samples Number 30 9 4 Setting Assay IDs 31 9 5 Setting Sample IDs 32 9 6 Working List 34 9 7 Reading Process 34 9 8 Summary Report and Results 36 9 9 Sample Reanalysis 38 10 Main Menu Options 10 1 Archive 42 10 2 Service 43 10 2 1 Import 44 10 2 2 System 44 Clinical Array reader CAR Manual Version 4 July 2009 gt Clinical Array Reader CAR User Manual GEN MICA 10 2 3 Backup Backup Path 45 10 2 4 Settings 49 11 FAQ 11 1 Updating software 50 11 2 Exporting results Backup path 53 11 3 Barcode Reader Characteristics and Installation 56 11 4 Dongle What it is and its use 56 Appendix 1 Abbreviations Used 57 Appendix 2 Symbols a
13. NS USED AS Array Strips AT Array Tube CAR Clinical Array Reader Fig Figure PCR Polimerase Chain Reaction PDF Portable Document Format generate by Adobe LIMS Laboratory Information Management System CLART Clinical Array Technology 13 APPENDIX 2 SYMBOLS AND WARNING SIGNS Yellow triangles indicate general warnings Warnings include the risk injury to the user or serious equipment damage This symbol indicates severe injury to the instrument operator due to interference of the user with moving parts of the CAR This symbol indicates the risk of loss of life or severe injury to the user due to high voltage gt oo This symbol indicates important information about the CAR and analyzing process Please read these instructions carefully This symbol indicates important information regarding ATs analyse 9 Yo Clinical Array reader CAR Manual Version 4 July 2009 59
14. al components Remove these screws before switching on the instrument for the first time and keep them for the posterior assembling of the screen No special tools are required to remove both screws Clinical Array reader CAR Manual Version 4 July 2009 7 Clinical Array Reader CAR User Manual GENE MICA Fig 1 Unlocking the CAR Position of the locking screws Place the white plastic cap attached in the hole left by the lock screw at the side of the CAR Fig 2 Fig 2 Covering the side hole left by the screw Should you need to send or ship your instrument the lock screws must be screwed securely in their positions To install the transport lock screws please follow these short instructions Turn off the instrument and remove the main power connection Slowly slide the tray holder manually fully towards the rear wall and place the screw at the rear of the CAR Then slide the tray holder fully to the right and place the screw on the side Tight them securely Clinical Array reader CAR Manual Version 4 July 2009 g Clinical Array Reader CAR User Manual CGS GEN MICA 4 3 ASSEMBLING THE SCREEN Touch screen is provided separately In order to connect it please follow these indications 1 Unscrew partially the two lower screws yellow and totally those marked in red all of them are placed on the rear side of the reader 2 Lean the screen over the reader fitting the screws with the positions
15. ckup only Backup and Delete lt lt Back Next gt gt Fig 61 List with selected results and the two backup options Press Next to finalize the backup process ay User can change the backup path at any time on the Settings menu See FAQ 10 2 4 SETTINGS Settings menu Fig 62 allows the user to Adjust data time manually Insert both data export and backup path Language only English Spanish French and Greek are available Default Assay Pneumovir HPV2 HPV2 MM Entherpex System Information Date Time 04 21 2009 15 36 05 Adjust Export Path F z Backup Path C Genomica data Backup Language English xi Default Assay CLART_Pneumovir_13 x CLART_HPV2_MM CLART_HPV2 CLART_Pneumovir_13 Fig 62 Setting Default Assay Clinical Array reader CAR Manual Version 4 July 2009 49 Clinical Array Reader CAR User Manual GEN MICA 11 FAQ 11 1 SOFTWARE UPDATING GENOMICA will provide the software necessary to read eventual updates or changes in the kits Please take care that the USB used does not contain any virus GENOMICA is not responsible about virus infections and CAR cleaning will not be consider as a part of the technical support Uncompress the zip file we have sent you on your pen drive Check that the pen drive now contains one folder called patch and one or more bat files with the name of the kit that you want to install It is important that all of
16. d alignment consist in positioning the upper left marker over the upper left metal marker and then adjusting the whole grid size by using the lower right metal marker Once the grid is squared properly according to array s dot micromatrix press Reanalyze button Fig 22 Zoom Cancel Fig 22 Placing metal markers according array s matrix Zoom tool placed on the upper right corner of the screen facilitates the reanalysis process We Zoom Fig 23 Manual grid alignment in strips using metal markers and zoom tool Once the metal markers are aligned press Reanalyse option Fig 23 and an image with the grid will show the final alignment If both dots and metal markers fits press Accepf lower right side If new adjustments are required press Retry Fig 24 Clinical Array reader CAR Manual Version 4 July 2009 26 Clinical Array Reader CAR User Manual GEN MICA fMo ololololololo ole OO oloo O O O O 6 6 O O o O O o O O O 6 G O 6 E O 6 e 6 o elelel e o e eBMoooccooo fe Zoom cept Fig 24 Grid alignment checking cy Manual grid alignment implies us
17. e sketch on the New Analysis menu This menu Fig 3 represents the cornerstone of the application since it allows the user to Set rack type choose between 96 wells microtitter plate AS or tubes adaptor AT Clinical Array reader CAR Manual Version 4 July 2009 14 Clinical Array Reader CAR User Manual GEN MICA Set the sample s number to be processed Set assay ID HPV Pneumovir Entherpex Set sample ID Sample Reference 8 1 SETTING SAMPLE S NUMBER The number of samples to be analysed can be introduced manually or automatically using a barcode reader It s not necessary to place the ASs in correlative positions for their lecture au Nevertheless when the CAR is included in a Clinical Array Processor CAP ASs must be placed in correlative order at the left side of the frame Automatic setting For the automatic setting of sample s number user will need a bar code reader connected to the CAR normal USB connection See FAQ GENOMICA will provide the user with specific bar codes for each assay Press Set Assay IDS option placed on the lower left side of the screen and all ASs will turn unselected on the New Analysis menu Fig 4 Select Rack 96 well plate x Select Rack 16 well plate 0 0 0 9 0 0 0 0 0 0 0 0 0 0 0 0 a ba Ly So Ca Ps a h d r o TEY 7 3 Pa k h eS 4 de q W T Y y oe a e See ae
18. ed in the process must be manually unselected by the user To unselect them proceed to press on the symbol which will turn into Q indicating the no selection of the AS The unselected AS appears on a light grey colour Fig 6 Select Rack 96 well plate Set Plate ID o 0 00 00 00 00 0 Edit Edit Edit Set Assay IDs Set Sample IDs pn Fig 6 Selection of AS number Sample s number should be always a whole multiple of eight which is the number of wells included in each AS Clinical Array reader CAR Manual Version 4 July 2009 16 Clinical Array Reader CAR User Manual GEN MICA 8 2 SETTING ASSAYS IDs The automatic setting of sample s number in the previous section allows the user to skip this step Setting Assays IDs Manually Software processes by default the CLART HPV2 assay To read or analyse any other GENOMICA S CLART Kit press the button on the New Analysis menu Fig 7a placed under each column Option Edit activates a new window Fig 7b that lets the user choose among those shown in the pull down menu User can apply same Assay ID to the whole plate from the Edit menu SelectRack 96 well plate iy 0 0 0 9 0 9 0 Po 2 gt i fe N S Y OY Y 4 OY J l ae a Si J Fs q i J b dh h A d a w ws SS b T J i YY Fk i _ gt oy Se Ea ee D i eb ae i oy N J gt
19. elect Assay CLART_HPV2_MM v Barcode Reader Assign Assay to all samples MON OOOOoWWO w Fig 34 Multiple assays choices Software gives also the possibility to select all tubes and edit the assay ID in all of them at same time In case of processing all samples with same assay user should select the Assign Assay to all samples on the Edit menu Fig 34 9 5 SETTING SAMPLES IDs In the same way that in previous sections setting Samples IDs can be carried out automatically or manually In case of no Samples IDs assigment software will set automatically a au correlative number to each sample from 1 to 12 starting with the sample placed in position 1A upper left Setting Samples IDs Automaticall aA Barcode reader is required to set Samples IDs automatically and barcodes must be set and printed before start setting the IDs Set and print barcodes Press Set Samples IDs option and read the previously assigned barcodes normally The software associates automatically a barcode to a sample position Samples with already assigned ID will appear in black Fig 35 Clinical Array reader CAR Manual Version 4 July 2009 32 Clinical Array Reader CAR User Manual GEN MICA Select Rack Set Manually Stop Import Fig 35 Setting Samples ID automatically Once finished importing IDs press Stop Import to return to the New Analysis menu Setting Samples IDs Manually Se
20. er s intervention in the results If a manual grid alignment is required an informative message will be shown in the run report The image has been reanalyzed Clinical Array reader CAR Manual Fig 25 Informative message indicating the manual reanalysis Version 4 July 2009 97 Clinical Array Reader CAR User Manual GEN MICA 9 PERFORMING ARRAY TUBES 9 1 PLACING ARRAY TUBES ON THE TUBE ADAPTOR The ATs adaptor is provided by GENOMICA and allows the user to place up to 12 ATs for analyzing For the adequate analysis of the tubes is necessary to place them properly on the adaptor Open the AT tube completely and turn back its cap Hold the tube in this position and introduce both tube and cap at the same time into the adaptor as shown in Fig 26 Tubes will be read in columns like the ASs starting from position A1 which is placed on the blunted square of the tube adaptor upper left position Fig 26 Placing tubes in the adaptor Select New Analysis option at the main menu of Genomica s application Fig 27 Service GEN MICA Archive Shut Down Fig 27 Selecting New Analysis at the main menu of Genomica s application Clinical Array reader CAR Manual Version 4 July 2009 28 Clinical Array Reader CAR User Manual GEN MICA 9 2 CHANGING RACK TYPE The software establishes the analysis of a whole 96 plate as default composed by up to 12 ASs In case
21. esults If a manual grid alignment is required an informative message will be shown in the run report The image has been reanalysed Clinical Array reader CAR Manual Fig 25 Informative message indicating the manual reanalysis Version 4 July 2009 4 Clinical Array Reader CAR User Manual GEN MICA 10 MAIN MENU 10 1 ARCHIVE By operating the Archive option Fig 46 user accedes to a results search engine Search must be enclosed between two dates established by the user a starting date and an ending date Fig 46 Set the searching dates required and press Next Select Start End Date O End Date GEN MICA Year Month Day aal asa kaalia ica FOON Shut Down lt lt Back Next gt gt Fig 46 Archive option on the main menu Fig 47 Menu to set the searching period time Search engine will find out all results contained between the two indicated dates In order to specify the search three extra searching parameters are available Fig 48 in a next menu Sample ID Search samples with same code or ID analyzed during the specified time period Assay ID Search samples processed with same procedure or assay during the specified time None Search for all samples included in the indicated time period Select Filters Sample ID Assay Name None lt lt Back Next gt gt JP IL IPE J Lees eS a a ee Esse
22. fs svary DeNpatch ensovat ion Metabone A lgorithn svalg Deo patch ensovat ion MetabonesMetabone svexp DeoNpatch ensovat ion Poeumovirs12Her with 5 SquareRefs svary De patch Sensovat ion Poeumovir Algorithn svalg Deow patch Sensovat ion PoeumovirPoeumovir svexp 9 Filets gt copied CLART HPU2 install ok Please restart the reader for changes to take effect Fig 70 11 2 EXPORTING DATA EXPORT PATH User can select where to export all data Results can be exported to a USB stick an external hard disk or to the LIMS just by selecting the proper export path Steps for exporting results to an USB stick Press Service option of the Genomica s main menu New Analysis Service GEN MICA Archive Shut Down Fig 71 Select Settings options on the secondary menu Clinical Array reader CAR Manual Version 4 July 2009 54 Clinical Array Reader CAR User Manual Oo a Robot Menu GEN MICA Reader Menu Pa I Fig 72 Browse directories by using the option and select directory D which corresponds to the USB stick System Information Date Time 04 07 2008 09 26 19 Export Path C Genomica Data CARExport Cc Backup Path C Genomica data Backup PEN Language English 7 Fig 73 Once selected the export path all exported data will be send using the export path Data exporting should be done from the Archive
23. ication control No signal Type 33 Type 5 gt D1 40204 Papilloma 000000040204 Genomic DNA control No signal Amplification E1 40204 Papilloma 000000040204 5 5 control No signal Type 6 Type 11 Type 16 Type 31 Type 61 Type 84 F1 40204 Papilloma 000000040204 Grid alignment failed The image could not be 5 PA i analysed G1 40204 Papiloma 000000040204 7 7 Genomic DNA control No signal Amplification 02 00000004020 3 control Passed Type 5 oa oO Grid alignment failed The image could not be 2 i 1000004020 H1 40204 Papilloma 000000040204 S analysed A2 40204 Papiloma 000000040204 9 9 Senom TIUA Cat Do den cones control No signal Type 33 Type 58 Genomic DNA control No signal Amplification B2 40204 Papilloma 000000040204 10 en p control No signal Type 6 Type 11 Type 16 Type 51 Type 61 Tyne 84 Fig 14 Summary report created by the CAR at the end of the analyzing process RESULTS To visualize a specific sample s result select the sample position on the microplate sketch Fig 15 by pressing twice on it Anew menu showing working list and results will appear on the screen Fig 16 On this menu following options are shown Export To export reports and results to an external server LIMS or USB Print If an external printer is connected via USB port the selected result s page or all results data pages including test image can be printed b
24. ing process in the CAR s screen Different colours for different analysis status Once finished the reading process user will be requested again to initialize the opening and closing procedures Figs 12 and 13 Results will be shown once the microplate has been removed from reader s and tray is closed 8 6 SUMMARY REPORT AND RESULTS SUMMARY REPORT The CAR generates a final report including following information Date and time Position of the sample Assay ID Sample ID Results for each sample User can look up the final report by selecting Report View option It s possible to print out this run report in PDF format just by pressing the print option on the upper right side of the screen Fig 14 Clinical Array reader CAR Manual Version 4 July 2009 97 Clinical Array Reader CAR User Manual GEN MICA Genomica SAU Working list s CAR CAP System Run ID 100002 J Date and time Sat Feb 02 09 59 57 2008 GEN MICA E Assay Assay Array ID bsy Sample reference Result Genomic DNA control Passed Amplification 2 i 00000004020 Ae Al 40204 Papilloma 000000040204 controt No signal Type 58 en Genomic DNA control Passed Amplification 2 i 0000040204 2 2 sien B1 40204 Papilloma 000000040204 EE No a ei Genomic DNA control Passed Amplification 2 i 000000040204 3 3 d C1 40204 Papilloma 000000040204 3 3 controt No signal Type 18 Type 84 Genomic DNA control Passed Amplif
25. ion Software Version Hardware version Hardware Version Serial Number Serial Number Fig 54 Information included in System menu Press Back to return to the Service menu 10 2 3 BACKUP BACKUP PATH Reader s storage capacity is limited to 40 Gb Backup option represents a useful tool to save both data results reports and CAR s settings Before saving results with the backup option user must set the Backup Path Clinical Array reader CAR Manual Version 4 July 2009 A5 Clinical Array Reader CAR User Manual GEN MICA SETTING BACKUP PATH Backup path sets up the target destination for the back up A remote folder or any other network drive can be specified To set the backup path go to Settings option Fig 55 Import System Fig 55 Selecting Settings option at the Service menu Press button System Information Date Time 04 07 2008 09 26 19 Adjust Export Path C Genomica Data CARExport C Backup Path C Genomica data Backup Language English oe Fig 56 Box position Browse directories and external devices connected to the CAR and select the destination Fig 57 Press OK when finished Clinical Array reader CAR Manual Version 4 July 2009 46 Clinical Array Reader CAR User Manual GEN MICA DA D Fig 57 Selecting directories or external devices Once se
26. irus Type 6 11 0 0078 0 0081 Negative a ae DPEN Type 26 0 0032 0 0033 Negative Print pete eave Passed Type 31 0 0064 0 0066 Negative Typene Negative Paseed Type 33 0 0086 0 0089 Negative Teete hemel Passeg a TPES Type 40 0 0095 0 0098 Negative Type 3 Negative Passed Type 42 0 0036 Type 39 pets 0 0027 Type 40 Type 44 0 0010 0 Type 42 Negative Passed same 0 0066 0 Type 43 Negative Passed 0 005 Typen Negative Bh Type 52 0 0051 0 0053 Negative Type 45 Type 53 0 0091 0 0094 Negative Type 51 Negative Passed Type 54 0 0049 0 0051 Negative Tuna ED Manntivn Darena te a Fig 19 Selecting Raw Data option Fig 20 Raw data and normalized values 8 REANALYZING SAMPLES After the analysis process red coloured wells where the grid alignment has failed can be reanalyze manually by user In the microplate sketch menu Fig 15 press on a red coloured sample position to activate the reanalysis main menu window Browse the proper Assay among those from the pull down menu Fig 21 and press Start Reanalyze Reanalyzation Information Select Assay CLART_HPV4 x CLART_HPV2_MM CLART_HPV2 Pneumovir Start Reanalyze Fig 21 Main menu of the reanalyze option for ASs By operating Start Reanalyze button user accedes to the reanalysis application Clinical Array reader CAR Manual Version 4 July 2009 25 Clinical Array Reader CAR User Manual GEN MICA The correct gri
27. ist prior to analyzing The working list includes necessary information for the samples traceability references assay ID position on the microplate 8 5 READING PROCESS TAKING IMAGES Reader s tray opening and closing processes are automatic Do not try to force or move it manually If neccessary press the emergency button placed on the rear side of the CAR User will be requested to initialize the process Fig 11 by pressing the Open Tray box Clinical Array reader CAR Manual Version 4 July 2009 20 Clinical Array Reader CAR User Manual GEN MICA GEN MICA Open Tray Fig 11 Message to initialize the reading process Once finished the opening process place the microplate ASs manually in the tray and operate the Close Tray box Fig 12 GEN MICA Close Tray Fig 12 Message to activate the closing process of reader s tray Reading procedure can be monitored in the screen since samples wells change their colour indicating the reading status Fig 13 Wells in green Sample already analysed Grid alignment agreed Wells in red Sample may be reanalyzed Grid alignment failed see Sample Reanalyze section below Wells in yellow Sample has not been analyzed yet Clinical Array reader CAR Manual Version 4 July 2009 21 Clinical Array Reader CAR User Manual GEN MICA Run Number 837 Plate ID 100002 35 Abort Fig 13 Monitoring the read
28. lect each position individually by pressing twice on it A new window representing a tactile key board will pop up on the screen Fig 36 Use it to set samples ID manually Selected Strip Position A1 Samples ID 98987 Cancel OK PPP POPUP LEE PE IE lee FE SE a rE NR TEA rie BiG JILL SHIFT Z Y i B N CLEAR Fig 36 Setting Samples IDs manually using the tactile keyboard The user can check the sample position at any time since it appears at the upper left side of the screen Once finished with the samples identification process press OK to go back to the New Analysis menu Fig 29 Then press NEXT Clinical Array reader CAR Manual Version 4 July 2009 33 Clinical Array Reader CAR User Manual GEN MICA 9 6 WORKING LIST The working list allows the user to check whether the already introduced information samples references assays is correct or not To change any data just press Back and repeat the setting process again see Set Samples IDs Manually and Set Assays ID Manually If all information is correct press Start to carry on with the reading process Fig 37 Working list Print Genomica SAU Rev 14 v l CAR CAP System Run ID 100003 Date and time Wed Feb 25 09 54 28 2009 GEN MICA Watt Ci 40207 C 000040040207 J3 AZ 40207 CLAF DM 90000004
29. llation process is done automatically by the device just by connecting it at the rear PS 2 or USB port Clinical Array reader CAR Manual Version 4 July 2009 57 Clinical Array Reader CAR User Manual GEN MICA Barcode Reader specifications recommendation Type Barcode Scanner Scanning Element CCD Light Source 660 nm red visible LED UPC E UPC A EAN 8 EAN 13 JAN UCC s EAN 128 ISBN ISSN Code 39 Code 93 canner Code 128 Interleave 2 of 5 MSI Industrial 2 of Decode Capability 5 Matrix 2 of 5 IATA 2 of 5 China Post Code Code 32 Codabar ABC Codabar CX Codabar Code 11 UK Plessey Telepen Scanning Speed 45 scans per second Decode Speed 100 scans per second Barcode Max Working Distance 1 Connectivity Interface PS 2 Power Voltage 5 VDC Requirements Consumption Decoded Standby lt 30 mA Working lt 95 mA Operating Temperature 0 to 50 Degrees C Conditions Humidity 20 to 80 non condensing 11 4 DONGLE WHAT IT IS AND ITS USE Dongle is the name of an electronic key with USB appereance It s placed in the rear side of the CAR and attached with a bridle Please insert the Dongle in a free USB port in order to let the CAR work Without the Dobgle CAR will start a maximun of 30 times before stop working Please do not remove the Dongle from the CAR Dongle Clinical Array reader CAR Manual Version 4 July 2009 58 Clinical Array Reader CAR User Manual GEN MICA 12 APPENDIX 1 ABBREVIATIO
30. n oa oa ae Ea ES ERS anar SPACE BAR Copy Paste 1 2 3 4 Fig 48 Available searching parameters Clinical Array reader CAR Manual Version 4 July 2009 A Clinical Array Reader CAR User Manual GEN MICA 10 2 SERVICE By operating the Service button 49 user accedes to a submenu containing several reader s options Fig 50 New Analysis Service GEN MICA a Fig 49 Selecting Service option from the Main Menu Import System Calibration Backup Robot Menu Reader Menu Fig 50 Multiple options of the Service submenu Clinical Array reader CAR Manual Version 4 July 2009 A3 Clinical Array Reader CAR User Manual GEN MICA 10 2 1 IMPORT This option Fig 51 is mainly used for eventual updates in the software version See section 9 1 for an extended explanations l gt a eres gt System Backup Reader Menu Fig 51 Selecting Import option at the Service Menu 4 DA D Fig 52 Multiple importing sources 10 2 2 SYSTEM System menu provides the user with information regarding software hardware and CAP s serial number Fig 53 Clinical Array reader CAR Manual Version 4 July 2009 AA Clinical Array Reader CAR User Manual GEN MICA A System ee Backup Reader Menu Fig 53 Selecting System option System Information Software vers
31. nd Warning Signs 57 Clinical Array reader CAR Manual Version 4 July 2009 3 Clinical Array Reader CAR User Manual GEN MICA 2 INTRODUCTION The Clinical Array Reader CAR has been designed for a quick detection and analysis of ASs This detection and analysis represent the second step of an ASs assay The first step of a complete ASs assay also involves experimental procedures sample preparation DNA extraction DNA amplification and target labelling specific hybridisation and visualization of the results The detection and analysis of ASs by the CAR is always performed immediately after developing the specific precipitation staining pattern on the ASs chip Developing of this pattern generates a dot micromatrix microarray on the glass slide at the bottom of the ASs This micromatrix can be detected and analysed by the CAR The results obtained by the CAR are automatically shown on the touch screen and can be printed or exported to your LIMS or pen drive by its USB connection Sample preparation DNA extraction and DNA amplification labelling of targeted Standard molecules laboratory equipment Specific hybridisation labelled sample incubation against AS chip Visualization conjugation and precipitation of the staining o O u A i z Ta Q gt K LLI Colorimetric Detection and Analysis sss ee A GEN MICA CAR Visualization CAR Clinical Ar
32. of analyzing ATs user should change the rack type To change the rack type just select the pull down menu Select Rack on the top of the screen and choose Tube adaptor Fig 28 instead of 96 well plate hey 4 hd isa sad cad hca bca lesd ba bea fea ea Led Leal Fig 28 Select rack option at the upper left side of screen A new window will pop up showing the tube adaptor sketch Fig 29 Select Rack Tube Adapter x Set Plate ID Use Tube Edit Assay Set Assay IDs Set Sample IDs Fig 29 Main menu when reading ATs Clinical Array reader CAR Manual Version 4 July 2009 29 Clinical Array Reader CAR User Manual GEN MICA 9 3 SETTING SAMPLE S NUMBER The number of samples to be analysed can be introduced manually or automatically by using a barcode reader Automatic setting For the automatic setting of sample s number user will need a bar code reader connected to the CAR normal USB or PS 2 connection Once the adaptor type is changed see Changing Rack Type above press Set Assay IDs option on the lower left side of the screen and all positions will turn unselected Fig 30 and 31 Read as many barcodes as sample s number you have and tubes will turn to the specific assay s colour combination Fig 32 Select Rack Set Plate ID Select Rack Set Manually i q F q if b p q q J q J q j q J Set Assay IDs Set Sample IDs Cancel Nex
33. ray Reader Clinical Array reader CAR Manual Version 4 July 2009 A Clinical Array Reader CAR User Manual GEN MICA 3 COMPONENTS DESCRIPTION gt Touch screen Reader s tray Function s gauge Performance Resolution on the sample 3 um Camera pixel resolution 8 3 Mpixel scan time for complete 96 well plate 2 3 minutes Dynamic Range 8 bits 255 greyscale Mechanical Additional 10 cm space must be provided in front of the instrument for microplate loading as well as an additional 10 cm space at the back for cables and ventilation Weight without tactile screen 15 Kg Replacement fan filter 80x8Omm NMB Part No F80 MR Environmental a Relative Humidity non Operating Temperature Storage Temperature 0 70 C 40 a 85 C 10 75 Clinical Array reader CAR Manual Version 4 July 2009 5 Clinical Array Reader CAR User Manual GEN MICA Electrical Electrical Specifications 5x20 mm 250V 1 6A slow 110 230 V AC a 50 60 150 W blow Hz Image storage format 8 bit BMP greyscale Results storage format CSV spreadsheet Internal PC Specifications Operating System Windows XP home edition Processor Intel Pentium II 1 6 GHz o mayor RAM Memory 1 GByte DDR 2 Hard Disk 40 GByte Connections VGA RS 232 mouse and keyboard Serial100 Base T Ethernet 3x USB2 Touch screen Characteristics 12 1 TFT LCD 800x600 264 K 12V VGA USB B Mechanical moun
34. t gt gt Stop Import T Fig 30 and 31 Setting Assay IDs automatically Select Rack Set Manually ag Stop Import Fig 32 Different colours combinations for each array type Clinical Array reader CAR Manual Version 4 July 2009 30 Clinical Array Reader CAR User Manual GEN MICA Manual setting In case of analyzing ATs those tubes that are not going to be processed should be unselected manually by pressing on g symbol The no selection of one tube will be indicated with symbol and the tube position will turn into a light dark grey combination Fig 33 Select Rack Tube Adapter v Set Plate ID Edit Assay Set Assay IDs Set Sample IDs Cancel Next gt gt Fig 33 Selected positions appearing in assay s specific combination Unselected positions turned into light dark grey combination 9 4 SETTING ASSAYS IDs The automatic setting of sample s number in the previous section allows the user to skip this step because assays ID s are already set Setting Assays IDs Manually Software processes by default the CLART HPV2 tubes To read or analyse any other GENOMICA S CLART KIT select the position by pressing on it and then press Edit Assay option Edit Assay Select the proper assay from those appearing in the pull down menu as shown Fig 34 Clinical Array reader CAR Manual Version 4 July 2009 3 Clinical Array Reader CAR User Manual GEN MICA Selected column 1 S
35. t VESA 75 Max current for 12VDC tactile screen supply 3 Amp Clinical Array reader CAR Manual Version 4 July 2009 6 Clinical Array Reader CAR User Manual GEN MICA CP ee Ol ay Please follow the indications described in this manual to set up the Clinical Array Reader CAR 4 1 PRECAUTIONS Verify that the instrument does not present any obvious sign of damage If any damage is found please contact immediately to your local GENOMICA s representative Prepare approximately 1 m free area in the lab for the installation of the CAR Once unpacked place your CAR in a standing position on the bench space you have just prepared Relocation of your CAR is only recommended by lifting it DO NEVER PUSH YOUR CAR Make sure that the CAR is placed on a stable and perfectly horizontal bench Before connecting it to the power supply make sure that your power line voltage corresponds to the operating voltage of 110 230 V AC at 50 60 Hz Do not install the CAR in the influence area of a heat or intense light source 4 2 UNLOCK LOCK THE READER In order to avoid system damages during transport internal mobile components of the CAR are locked In order to unlock the reader remove the big screw on the back and side Readers contain two locking systems one on the rear side and one in the right side During transportations the position of the tray holder is fixed by lock screws in order to protect the mechanic
36. t the back up path user can backup results by pressing the Backup button Fig 58 Import System Backup w Reader Menu Settings Fig 58 Selecting Backup option Next select Results Fig 59 on the touch screen and then Next Clinical Array reader CAR Manual Version 4 July 2009 47 Clinical Array Reader CAR User Manual GEN MICA Select Backup Type GEN MICA Results O Configuration data lt lt Back Next gt gt Fig 59 Selecting Results backup To speed up data backup user can introduce a time interval All results reports included between the starting date and ending date will be back up Fig 60 Then press Next Select Backup Start End Date Start Date 2008 04 07 End Date Year Month Day iil Lata hasad biti cal la Fig 60 Introducing dates for the backup All selected runs are shown on an extra menu Fig 61 Both options Backup only or Backup and Delete are possible User decides whether to keep data on the CAR s reader or erase the already saved results Clinical Array reader CAR Manual Version 4 July 2009 Ag Clinical Array Reader CAR User Manual GEN MICA All None Run Date a lt 922 4 2 2008 1 27 30 PM 923 4 2 2008 1 35 42 PM 925 4 3 2008 11 53 11 AM 926 4 4 2008 1 03 48 PM 927 4 4 2008 3 56 33 PM Ml QPR ALAIPNNR 2 52 5R PHA lt Ba
37. terial must not be used with this equipment Only use Array Strips or Array Tubes processed in accordance to the AS AT experiment protocols provided by GENOMICA Use of this equipment in a different way of that described in this manual will result in lost of your warranty rights Please take care that the USB used for software updates or results export does not contain any virus GENOMICA is not responsible about virus infections and CAR cleaning will not be considered as a part of the technical support Clinical Array reader CAR Manual Version 4 July 2009 11 Clinical Array Reader CAR User Manual GEN MICA 7 SWITCHING ON OFF THE CAR 7 1 SWITCHING ON Connect the Dongle to a free USB port see FAQ 11 4 Plug in the reader and switch it on The main switcher is on the rear right side of the device Once pressed function gauge will display a yellow light Press then the secondary switcher on the front side of the CAR and wait until function s gauge turns green Once your CAR is switched on the instrument will initialise during this time the instrument will verify its good performance by a series of self tests After finishing this set of self tests the Main Menu screen will appear on the touch screen which will allow to start reading and analyzing the samples IEN MICA Fig Secondary switcher at the front side 7 2 SWITCHING OFF Press first Shut Down box on the main menu to switch off the
38. tive Passed Type 35 Negative Passed 0 0098 Type 40 we mae ae Type 42 Negative Passed m Type 0 si 0 0011 ser Type 43 Negative Passed Type Bsa a Type 44 Negative Passed ype st 0 0056 0 0058 A A i Type 45 Passed yese ame am an Type 51 Negative passed Type 53 0 0091 0 0094 Negative Tunn ED Niet a TERETA Type 54 0 0049 0 0051 Negative pe RawData Ss r ii 4 Fig 19 Selecting Raw Data option Fig 20 Raw data and normalized values 9 9 SAMPLE REANALYSIS After the analysis process red coloured wells where the grid alignment has failed can be reanalyze manually by the user In the tube adaptor sketch menu Fig 41 press on a red coloured sample position to activate the reanalysis main menu window Fig 42 Clinical Array reader CAR Manual Version 4 July 2009 38 Clinical Array Reader CAR User Manual GEN MICA Run Number 88 Plate ID 100003 View Report O O Fig 41 Grid alignment failed positions when performing Pneumovir Kit When performing CLART HPV2 in tubes alignment must be carried out with the biotin markers instead of Metal Markers Protocol stays the same See Fig 40 The correct grid alignment consist in positioning the upper left marker over the upper left array s dot and then adjusting the whole grid size by using the lower right marker Once the grid is squared properly according to array s dot micromatrix press Reanalyze button
39. vir_install bat D Fig 68 Select the kit that you want to install and press Import E C amp DA patch Metabone_install bat Pneumovir_install bat D HPV2_install bat Fig 69 Installation will be finished when a new window pops up indicating that the process has been completed successfully Please close that window by pressing Cross button on the top right square If the installation of more kits is required repeat the process from step 4 Once finished get back to the Main Manu and reinitialize the reader In case that an error occurs during the installation process please contact the technical service Do not forget to attach the appearing error messages in your assistance request Clinical Array reader CAR Manual Version 4 July 2009 53 Clinical Array Reader CAR User Manual GEN MICA GY C windows system3 cmd exe 2 x E D 5patchGenomicasScripts gt runreport error html DeNpatch Genomica Scripts runre port py DeNpatchGenomica cripts workinglist error html DeNpatch Genomica Scripts workinglist py De patch Genomica Scripts workinglist tmp html De patch GenomicaScripts sworkinglist voc 1633 De patch Genomica Scripts sworkinglist voc 1634 38 Filets copied D 5patchsfensovat ions CLART_HPU2 120er with 5 CircleRefs svary D2 patch ensovat ion CLART_HPU2 Algorithm svalg De patch Sensovat ion CLART_HPU2S CLART_HPUS svexp DeNpatch ensovation Metabone 12Her with 5 CircleRe
40. y operating the Print button See FAQ Image To visualize the image obtained by the CAR Fig 18 Raw Data To consult raw and normalized numerical values obtained in the analysis of the samples Clinical Array reader CAR Manual Version 4 July 2009 23 Clinical Array Reader CAR User Manual GEN MICA Virus ype 6 Passe ype 11 Passe ype 16 Passe ype 18 Passe ype 26 Passe Image ype 31 Passe ype 33 Passe ype 35 Passe Type 39 Passed ype 40 Passe ype 42 Passe ype 43 Passe ype 44 Passe Tuna ED Mananta Darren H i gt P 3 o D alajaja alolaolalajalala Fig 16 Results menu By selecting the Image option user accesses to the image corresponding to the analyzed sample Fig 17 and 18 Virus s Contr Export Type 6 Passe ype 11 Passe Print Type 16 Passe ype 26 Passe Type 31 Negative Passed Type 33 Passed Type 35 Passed Passed Type 40 Negative Passed x Image ype 18 Passe Q ao Oo po pa oe Oek ai Type 45 Type 51 Negative Passed Tuna ED Manntivn Narnend Fig 17 Selecting Image option Fig 18 Example of a HPV assay performed in strips metal markers Clinical Array reader CAR Manual Version 4 July 2009 24 Clinical Array Reader CAR User Manual GEN MICA By selecting Raw Data option user accesses to the numerical values measured for each virus type Fig 19 and 20 v
41. y time since it appears at the upper left side of the screen Once finished with samples identification process press OK to go back to the New Analysis menu Fig 2 Then press NEXT 8 4 WORKING LIST The working list allows the user to check whether the already introduced information samples references assays is correct or not To change any data just press Back and repeat the setting process again see Set Samples IDs Manually and Set Assays ID Manually If all information is correct press Start to carry on with the reading process Fig 10 Working list I J epep ae oe i Date and time Thu Jan 10 20 47 09 2008 GEN MICA Pos Ey Assay Array ID jiss Sample reference Result Al 40204 Papilloma 000000040204 1 654684 Bi 40204 Papilloma 000000040204 2 16687 C1 40204 Papilloma 000000040204 3 4987 Di 40204 Papilloma 000000040204 4 4687 E1 40204 Papilloma 000000040204 5 0136 F1 40204 Papilloma 000000040204 6 13786 Gi 40204 Papilloma 000000040204 7 34684 H1 40204 Papilloma 000000040204 8 1 68789 A2 40204 Papilloma 000000040204 9 1657345 B2 40204 Papilloma 000000040204 10 1678 C2 40204 Papilloma 000000040204 11 04797654 D2 40204 Papilloma 000000040204 12 996432175 E2 40204 Papilloma 000000040204 13 1657486 Operation Mode Process and Reading Reading Fig 10 Working l
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