EXT-02B manual ver1_3 - NPI Electronic Instruments
Contents
1. EXT 02 B has a differential input with high input impedance to avoid noise The output voltage signal can be HIGH PASS and LOW PASS filtered and 1 available either AC HIGH PASS and LOW PASS filtered with variable gain or DC coupled with a fixed gain of 10 The EXT 02 B is built in a master configuration and a slave configuration The master configuration has got a built in power supply and is capable of powering up to three EXT 02 B in slave configuration Furthermore one audio monitor AUD 08 B and one electrode resistance meter REL 08 B All these instruments are interconnected via the system bus which combines power and data lines 2 3 Description of the Front Panel 10 30 100 Ik 3k 5k gt e ON 100 gt 300 ON i 0 1 500 ie 20k A DIFF NOTCH HIGHPASS FILTER Hz GAIN LOWPASS FILTER Hz DIFF NOTCH HIGHPASS FILTER Hz GAIN OFF SINGLE OFF SINGLE oe i ia L2 INPUT MODE 48 INPUT MODE amp CHANNEL A ZERO ADJ OFFSET ZERO ADJ OFFSET E 1 0 00 190 qw OWN AC C OFF DC x10 TILIN AC DC x10 STIMULUS CONTROL OUTPUT HEADSTAGE STIMULUS CONTROL OUTPUT 2 9 2 YD Figure 1 EXT 02 B front panel view version 1 3 page 4 EXT 02 B User Manual In the following description of the front panel elements each element has a number that 1s related to that in Figure 1 The number is followed by2. Electronic npi Instruments for the Life Sciences oaa e made OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE EXT 02 B EXTRACELLULAR AMPLIFIER EXTRACELLULAR AMPLIFIER CH HIGHPASS FILTER Hz gt L DEF SNG ZR MODE CHANNEL A VERSION 1 3 npi 2014 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com EXT 02 B User Manual Table of Contents ROSBORN mE 3 2 S LER PAL ets 4 CONDON T 4 PAPE SS UU etic 4 2 5 Desenptiom Ob INE Front Pane 4 ZA Descriptionor the Rear eine aeons cana 8 2 35 Hedd to dpt KIM 10 Elemen oo a AER 10 Su PCL AU OM 10 3 1 Switching between recording and 10 3 2 Extracellular Recordings Single ended vs differential sssssssssss 11 3 3 System Bus interconnecting Inu HM SUE POOR E ads 11 _ WE cuis R 14 version 1 3 page 2 EXT 02 B User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical pu
3. a TTL input signal to switch the headstage from recording mode TTL low to stimulation mode TTL high 12 STIMULUS CONTROL switch Toggle switch to switch the headstage from recording mode OFF position to stimulation mode ON position 13 STIMULUS INPUT connectors 2mm banana jacks for connecting the high voltage signal for stimulation This input signal is fed to the headstage only if either the TTL at 11 is high or the switch 12 is in ON position Note The stimulus ground black connector and the analog system ground are connected internally 14 HEADSTAGE connector Connector for the standard sized headstage Banana plug 4 mm providing ground The plug is connected internally to the TH shields of the BNC connectors version 1 3 page 7 EXT 02 B User Manual 2 4 Description of the Rear Panel CHANNEL B CHANNEL A MONITORING MONITORING POWER e GAIN LOWPASS HIGHPASS SYSTEM BUS GAIN LOWPASS HIGHPASS ON OFF m lt Soe ee ei aA Caen ths GR D POWER SUPPLY C C 18V AC 1 8A J 3 CM Cie Jo Ju es m 4778 3 6 2 7 3 6 i SYSTEM BUS 2 7 ee electronlc GmbH DE 71732 Tamm HANNEL ADDRESS CHANNEL ADDRESS www npielect onic com X Figure 2 EXT 02 B rear panel view Since the rear panel elements for the two channel EXT 02B are identical for each channel A
4. and B with identical functions and labels these elements are numbered and described only once for channel B MONITORING section 1 GAIN 1V 4V connector BNC connector providing a voltage monitoring the position of the GAIN of the AC OUTPUT 1 V to 4 V 1 V STEP 2LOWPASS 1V 6V connector BNC connector providing a voltage monitoring the position of the LOWPASS FILTER switch 1 V to 6 V 1 V STEP 4 HIGHPASS 1V 6V connector BNC connector providing a voltage monitoring the position of the HIGHPASS FILTER switch 1 V to 6 V 1 V STEP 3 CHANNEL ADDRESS rotary switch only in slave configuration Switch for selecting the address in the system bus to which the recording channel will be linked This switch is only functional in amplifiers with slave configuration Master amplifiers will always have the addresses channel A and 1 channel B Important Each recording channel has to have its dedicated address i e no more than one channel is allowed on a single address number Otherwise incorrect output signals might occur or the system might even be damaged version 1 3 page 8 EXT 02 B User Manual 5 SYSTEM BUS connector see also chapter 3 3 50 pin connecter providing power lines and data lines for connection of three EXT 02 B in slave configuration to one EXT 02 B in master configuration An electrode resistance meter REL 08 B and an audio monitor AUD 08 B can also be connected via this syst
5. charge and must therefore be handled with care Electrostatic discharge can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals version 1 3 page 3 EXT 02 B User Manual 2 EXT 02 B 2 1 Components The following items are shipped with the EXT 02 B system Amplifier EXT 02 B User manual Headstages Power Supply System Bus cable slave configuration only lt 5 5 2 2 System Description The EXT 02 B is designed for extracellular recordings of small voltage signals It has a switchable headstage which enables the researcher to record and to stimulate through the same electrode The high voltage signal for stimulation is fed into the amplifier via connectors at the front panel separately for each channel Switching between recording and stimulation can be done via a TTL signal or by manual switching The
6. ctrode holder BNC jack PEL REF GND suBcuck Ground connector Jack REF m Holding bar single ended measurement configuration Figure 4 headstage connections A differential measurement B single ended measurement Hint The REF connector of the headstage can also be set to GND by setting the INPUT MODE switch 2 Figure 1 to SINGLE E version 1 3 page 12 EXT 02 B User Manual 4 Troubleshooting FAQ 50 Hz noise 50 Hz noise version 1 3 Magnetic field generated by Place power supply as distant as possible power supply from the amplifier Undervoltage in power supply If the power supply has adjustable voltage generates ripples in supply make sure it 15 set to the right values Make voltage of the amplifier also sure that the power supply is capable of providing enough current Best use the provided power supply page 13 5 Technical Data Input headstage Differential Input Input Capacitance OFFSET compensation NOTCH Filter HIGHPASS Filter LOWPASS Filter GAIN Output Power Supply Dimensions Headstage Input headstage Headstage Size Holding Bar version 1 3 EXT 02 B User Manual gt 10 range 1 V CMR gt 90 dB at 1 kHz tested with input resistance 30 pF set by potentiometer range 1 V 50 Hz Europe 60 Hz USA single pole attenuation 6 dB octave co
7. e researcher to record and to stimulate through the same electrode Switching between recording and stimulation mode is achieved either by a TTL signal fed into TTL IN connector 11 Figure 1 or by manually toggling a switch 12 Figure 1 In recording mode the signal detected at the electrode 1s pre amplified in the headstage and fed into the EXT 02 B for further amplification and filtering In stimulation mode the preamplifier in the headstage is disconnected from the electrode and the stimulation line 1s connected to the electrode instead The signal is fed into the stimulation line at the black and red input connectors at the front panel see 13 Figure 1 version 1 3 page 10 EXT 02 B User Manual 3 2 Extracellular Recordings Single ended vs differential Extracellular measurements are mostly done in slices or in vivo where distortions of the signal caused by other instruments and the animal itself are very common Additionally extracellular signals are very small and have to be amplified enormously The drawback is that noise 15 amplified as well Therefore each channel of the EXT 02 B is equipped with differential input that minimizes noise pick up Differential means that the signal for the amplifier is the difference between the positive at the headstage and negative REF at the headstage input of the amplifier This results in canceling of all signals which both electrodes record e g noise For differe
8. em bus 6 POWER ON OFF switch only in master configuration Switch to power the EXT 02 B amplifier 7 POWER SUPPLY connector only in master configuration Connector for an external power supply 18 V AC min 1 5 A Important If you encounter noise when operating the EXT 02 B try to place the amplifier as far away as possible from the power supply This will avoid magnetic coupling of the power supply to the amplifier version 1 3 page 9 EXT 02 B User Manual 2 5 Headstage headstage npi electronic GmbH electrode holder D 71732 Tamm REF 7 a www npielectronic com AMPLIFIER HEADSTAGE TYPE ak XI XI 02 EXT O2BP Figure 3 headstage and electrode holder optional and of the EXT 02 B Headstage Elements BNC connector for the electrode holder measuring electrode REF SMC connector for the reference electrode GND Ground connector holding bar A U Nm On request Pg can be implemented using 1 mm or 2 mm banana jacks or using SMC connectors Very Important EXT 02 B headstage are always labeled 02 B see Figure 3 and must not be exchanged with headstages from other npi electronic EXT amplifiers e g the modular EXT 10 2F or EXT 10C or desktop EXT 02F which are labeled EXT or EXT 02 3 Operation 3 1 Switching between recording and stimulation The EXT 02 B has a headstage which can be switched from recording mode to stimulation mode This enables th
9. ntial measurements both inputs are connected to electrodes using cables with grounded enclosure or electrode holders PEL 1 connected to the measuring electrode and REF to the reference electrode The experimental chamber is grounded by an Ag AgCl pellet or an AGAR bridge connected to GND of the headstage see Figure 4 Important differential measurement is not required single ended measurement configuration the REF input must be connected to ground GND at the headstage see Figure 4 or the INPUT MODE switch 2 Figure 1 must be set to SINGLE The amplifier is in an undefined state if the REF is left open and can go into saturation making reliable measurements impossible 3 3 System Bus interconnecting devices Connecting multiple devices The System Bus interconnects all amplifiers EXT 02 B in master and slave configuration and monitoring devices REL 08 B AUD 08 B It provides both power lines and all signal lines The number of signal lines depends on the number of connected amplifiers All devices of the B series EXT 02 B REL 08 B AUD 08 B have two SYSTEM BUS connectors at the rear panel 5 Figure 2 To interconnect two or more devices simply use the provided cable and plug it into one of the SYTEM BUS connectors of each device This will result in a chain of devices which has a maximum length of six 1 x EXT 02 B master 3 x EXT 02 B slave 1 x AUD 08 B 1 x REL 08 B The order in which the device
10. quency of the single pole Afix LOWPASS filter with 6 dB octave 20k Corner frequencies Hz 500 1k 5k 10k 20k LOWPASS FILTER Hz 7 OFFSET potentiometer f 0 Potentiometer for OFFSET compensation for the measured potential at the E DC x10 OUTPUT 9 OFFSET 8 DC x10 OUTPUT BNC connector providing the measured DC signal with a fixed amplification of 10 This signal is not filtered Use of this connector allows for instance measuring the signal AC signal is provided at AC OUTPUT 9 while watching ocxi0 offset simultaneously OUTPUT 9 AC OUTPUT connector BNC connector providing the recorded and conditioned signal The signal is amplified by the factor set at 5 and filters with the corner frequencies set at 4 and 6 AC OUTPUT 10 ZERO ADJ trimpot Trimpot for compensating for amplifier offsets If the baseline is not zero even if the e input is grounded INPUT MODE switch 3 set to OFF this offset can be ZERO ADJ compensated for Compensation procedure I Set the INPUT MODE switch 2 to OFF to ground the inputs and set the HIGH PASS FILTER to 300 Hz I Set the GAIN range switch to 1k and adjust the baseline to zero using the trimpot I Set the GAIN range switch to 10k and adjust the baseline again if necessary version 1 3 page 6 EXT 02 B User Manual STIMULUS CONTROL section Es Em un NT LUE 11 TTL IN connector BNC connector for
11. rner frequencies Hz 0 1 1 10 30 100 300 single pole attenuation 6 dB octave corner frequencies Hz 500 1k 5k 10k 20k rotary switch 10 100 1k 10k range 10 V into 1 1 V into 50 Q 18V AC min 1 8 305 x 180 x 105 mm W x D x H gt 10 range 1 V 70 x 26 x 26 mm length 150 mm diameter 8 mm page 14
12. rposes e g for diagnosis or treatment of humans or for any other life supporting system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is designed for use in scientific laboratories and must be operated by trained staff only General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic
13. s are chained up is not important Since it is a bus system all positions are treated equally Channel addresses When using monitoring devices REL 08 B AUD 08 B it 1s important that each recording channel has its dedicated address i e that there is no more than one channel on a single address number This can be achieved by selecting a channel s address number with the CHANNEL ADDRESS rotary switch 3 Figure 2 on the rear panel of the EXT 02 B in slave configuration The EXT 02 B in master configuration does not have a CHANNEL ADDRESS rotary switch Its recording channels will always addressed to 0 for channel A and 1 for channel B Therefore the selectable addresses for the EXT 02 B in slave configuration begin at 2 and go up to 7 Each recording channel can be monitored either acoustically with the AUD 08 B or its electrode resistance can be measured with the REL 08 B These devices have a CHANNEL SELECT rotary switch on their front panel whose numbers correspond to the channel address number selected at each channel of the EXT 02 B version 1 3 page 11 EXT 02 B User Manual A Experimental chamber Slice REF N AgAgC electrode __ X pellet Electrode holder BNC jack PEL REF GND _ SU BCLICK Ground eonnector jack REF Holding bar differential measurement configuration Experimental y _ chamber 5 UMP Ele
14. the name in uppercase letters written on the front panel and the type of the element in lowercase letters Then a short description of the element is given Since the front panel elements are identical for each channel A and B with identical functions and labels these elements are numbered and described only once for channel A Signal is the recorded signal REF is the signal from the reference input of the headstage 1 POWER LED D LED indicating that the EXT 02 B is powered on 2 INPUT MODE switch Rotary switch to select the input mode OFF st SN GU OFF both inputs of this channel Signal REF are grounded Lg E can be used to adjust the offset of the AC output DIFF differential mode signal is measured against REF ANPUT MC SINGLE single ended measurement signal is measured against GND 3 NOTCH switch Switch that enables upper position or disables lower position the NOTCH filter 4 HIGHPASS FILTER Hz switch 6 position switch for selecting the corner frequency of the single pole HIGHPASS filter with 6 dB octave Corner frequencies Hz 0 1 1 10 30 100 300 5 GAIN switch 10 1k 4position switch for selecting the GAIN of the output signal at the 10 0219 OUTPUT connector 10 Amplification factors x10 x100 x10k version 1 3 page 5 EXT 02 B User Manual 6 LOWPASS FILTER Hz switch 6 position switch for selecting the corner fre
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