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Multipette® M4 · Repeater® M4
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1. Symbol Meaning Handling 1 Actions in the specified order List Step in the figures Text Name of fields in the software 0 Useful information 1 4 Glossary A Adapter advanced Connecting piece for the dispenser when using Combitips advanced 25 mL and 50 mL Additional volume The total of the remaining stroke and the reverse stroke B Biopur Eppendorf Biopur9 is an Eppendorf AG purity level for consumables Eppendorf Biopur meets the requirements for standard products e g precision accuracy wetting behavior tightness Eppendorf Biopur also fulfills the requirements with regard to sterility absence of ATP PCR inhibitors human and bacterial DNA pyrogens DNase and RNase Consumables with the Biopur purity grade are controlled and certified by an external laboratory Certificates are available for downloading from our webpage www eppendorf com Operating instructions Multipette M4 Repeater M4 English EN C Coding The dispenser uses the Combitip coding to detect the Combitip s maximum volume Color code The color code displays the maximum volume Combitip advanced Consumables for all Eppendorf Multipettes and Repeaters With the Combitip advanced the dispenser becomes an operational dispenser Combitips advanced are intended for single use Combitips advanced consist of a piston and a cylinder and function according to the positive displacement principle For Combitips advanced
2. Troubleshooting Multipette M4 Repeater M4 39 English EN 6 1 3 Display Symptom message Cause Remedy Display remains No Combitip advanced K Insert Combitip dark used advanced Battery fully discharged Replace the battery Combitip sensor faulty Call service Movement sensor faulty Call service Number of the steps Incorrect interpretation of K When selecting the shown on the display is incorrect the information volume the possible dispensing steps are displayed After the reverse stroke the dispensing steps that were carried out are displayed Operating lever not fully pushed Always push the operating lever down as far as it will go 40 Troubleshooting Multipette M4 Repeater M4 English EN 6 1 4 Error codes Symptom message Cause Remedy C02 Err Combitip advanced very Do not bend or distort C03 Err heavily bent or distorted inserted Combitip during and after advanced inserting Combitip advanced 1 Press the filling lever incorrectly or not fully down all the way inserted 2 Operate the operating lever to eject the Combitip advanced 3 Check the Combitip advanced or the Adapter advanced for damage to the coding Combitip advanced not K Operate the operating fully released lever again fully and centered to eject the Combitip advanced Coding on the Combitip Insert a new Combi
3. Order no Order no North International America Description 0030 059 506 Combilong Aspirating aid for removing liquids from volumetric flasks and tall bottles 2 pieces Combitube Aspirating aid for removing liquids from volumetric flasks and tall bottles 022261550 2 pieces Combitips advanced Rack 1 piece 0030 089 758 0030089758 Eppendorf Quality Patents Multipette M4 Repeater M4 57 English EN 11 Patents U S Patent No 5 573 729 Is used by Multipette amp M4 and Repeater M4 U S Patent No 5 591 408 Is used by Multipette amp M4 and Repeater M4 U S Patent No 5 620 660 Is used by Multipette M4 and Repeater amp M4 U S Patent No 8 114 361 is used by Multipette amp M4 and Repeater M4 58 Index Multipette M4 Repeater M4 English EN Index A Adapter advanced LO ele E Lu Le NEE Autoclaving eene ten same B Battery Replace sees eee C Cleaning TTT Cleaning agents Isopropano sese Color code Color code testet ses Combitip Adapter ek Assembly Color code iei etes Unpacking eene Volume table Combitip rack Autoclaving sssseseeeseesseeerrssesseeee D Delivery D ckage Displays karote eter tette EE Sleep function oe eee 13 Disposal pes 50 F Free jet dispensing 35 H Holder Carousel tct ens 22 Malle ds 21 L Liquid ASDIFatlOD s ceret 33 Dis
4. 0 5 8 1 4 e 1 5 3 2 2 e 2 5 2 3 2 e 3 5 2 24 1 Operation Multipette M4 Repeater M4 35 English EN 1 Press the operating lever to trigger the reverse stroke When the reverse stroke is completed the display will stop blinking The steps display is set to 0 During the subsequent dispensing procedures the completed steps are displayed n steps LI m T bU ul 5 5 2 Dispensing liquid Prerequisites The liquid dispensing angle should always be as steep as possible A dispensing angle greater than 45 can result in an incorrect dispensing volume during the final dispensing steps 1 Place the tip of the Combitip at a steep angle on the inner wall of the target tube wall dispensing or hold the Combitip over the target tube free jet dispensing 2 Push the operating lever down as far as it will go The display shows the steps and the number of dispensing steps performed steps 5 n T U ul O The faster you push the operating lever down the faster the liquid is dispensed Adjust the liquid dispensing to the tube geometry to prevent liquid splashing out of the tube For highly viscous liquids always operate the operating lever very slowly Operation 36 Multipette M4 Repeater M4 English EN 3 Let the operating lever slide back fully to its initial position 4 Push the operating lever down again to perform the next dispensing
5. 1 mL 2 5 mL 5mL 10mL 25 mL 50 mL 16 Product description Multipette M4 Repeater M4 English EN 2 6 Delivery package Quantity Description Multipette M4 Repeater M4 Operating manual Mini CD with operating manual and additional information Combitip advanced 2 5 mL Holder for Multipette M4 Repeater M4 Battery fitted s Eppendorf certificate 2 7 Materials NOTICE Aggressive substances may damage dispensers Combitips and accessories Check the chemical resistance when using organic solvents or aggressive chemicals Please note the cleaning instructions The parts of the Multipette M4 Repeater M4 that can be accessed by the user are made from the following materials Component Material Housing parts Improved polypropylene PP Filling lever operating lever Improved polypropylene PP dyed Viewing window Polycarbonate PC Volume selection dial Acrylonitrile styrene copolymerisate with polycarbonate ASA PC Other external components Polyetherimide PEI Polybutylene terephthalate PBT Polyetheretherketone PEEK Acrylonitrile styrene copolymerisate with polycarbonate ASA PC Silicone Product description Multipette M4 Repeater M4 17 English EN Component Material Holder Acrylonitrile styrene copolymerisate with polycarbonate ASA PC Combitip advanced Mate
6. the piston movement maximum number of possible Aspiration A or dispensing V dispensing steps are displayed During dispensing the dispensing steps that have been carried out are Battery symbol Will be displayed briefly after the battery is inserted If the end of the displayed battery service life approaches the 2 Volume charging status will be displayed In uL or mL O If the display flashes the current or next operating step is nota dispensing step 14 Product description Multipette M4 Repeater M4 English EN 2 3 Features The Multipette M4 Repeater M4 dispenser can only be operated with a Combitip advanced This makes the Multipette M4 Repeater M4 dispenser a positive displacement dispenser Depending on the Combitip advanced used you can dispense volumes in the range from 1 ul to 10 mL The dispensing volume is set with the volume selection dial and shown on the display For each Combitip advanced 20 different dispensing volumes can be set with the volumen selection dial Depending on the dispensing volume 5 to 100 dispensing steps can be carried out with a Combitip advanced that is filled to capacity The display shows the number of possible or completed dispensing steps The display of the dispensing volume and the possible dispensing steps is made possible by the automatic detection of the size of the Combitip advanced Automatic detection is via a coding on the Combitip advanced and a sensor ring in the disp
7. 5 1 2 Unpacking the Combitip advanced O To ensure maximum protection against contamination use Combitip advanced of the Sterile and Biopur purity grades straight after opening the packaging K Open the packaging at the location indicated 26 Operation Multipette M4 Repeater M4 English EN 5 1 3 Assembling the Combitip advanced and Adapter advanced Combitips advanced with a maximum volume of 0 1 mL 10 mL can be used immediately Combitips advanced with a maximum volume of 25 mL or 50 mL can only be used with the corresponding Adapter advanced Adapter advanced and Combitips advanced have the same color code The maximum volume is also listed on the neck of the Adapter advanced NOTICE Sensor damage due to damaged or worn adapter Always put the adapter and Combitip together outside of the dispenser Do not used damaged or worn adapters Do not use adapters with damaged coding 1 Place the adapter on the Combitip 2 Tighten the adapter Operation Multipette M4 Repeater M4 27 English EN 5 1 4 Inserting the Combitip NOTICE Device damage due to incorrect handling of the inserted Combitip Insert the Combitip straight into the dispenser from below Do not rotate the inserted Combitip K Never grip the dispenser using the Combitip O If you keep pushing the operating lever while inserting the Combitip it will be easi
8. LRL RE Re ce t D ER d 17 E WEE 18 3 1 Intended Use 1 raa y eese eeu a 18 3 2 Warnings for intended use 18 3 3 Information on product liability 20 AC Installation se sdin eeu de tete eerte E 21 4 1 Using the holder supers uns eee STEEN ea Pete 21 4 1 1 Mounting the holder on a wall 21 4 1 2 Inserting the holder into the pipette carousel 22 Table of contents 4 Multipette M4 Repeater M4 English EN 5 JOp ratioiv MC E EE 23 5 1 Inserting the Combitip advanced 23 5 1 1 Selecting the Combitip advanced 24 5 1 2 Unpacking the Combitip advanced 25 5 1 3 Assembling the Combitip advanced and Adapter advanced 26 5 1 4 Inserting the Combitip 27 5 1 5 Picking up Combitips out of the rack 28 5 2 Setting the volume 29 5 2 1 Setting the volume before dispensing 29 5 3 Using the step counter 30 5 3 1 Step counter display for Combitip filled to capacity 30 5 3 2 Sequential step counter display for a Combitip that has been filled to capacity several times 31 5 3 3 Resetting the step counter to Zero when Combitip is filled repeatedly 32 5 3 4 Step counter display if Combitip
9. Quality i 0030089510 Sterile individually wrapped 0030 089 618 0030089618 Biopur individually wrapped 0030 089 766 PCR clean Combitips advanced 0 2 mL 100 pieces 0030 089 413 0030089413 Eppendorf Quality 0030089529 Sterile individually wrapped 0030 089 626 0030089626 Biopur individually wrapped 0030 089 774 PCR clean Combitips advanced 0 5 mL 100 pieces 0030 089 421 0030089421 Eppendorf Quality 0030089537 Sterile individually wrapped 0030 089 634 0030089634 Biopur individually wrapped 0030 089 782 PCR clean Combitips advanced 1 0 mL 100 pieces 0030 089 430 0030089430 Eppendorf Quality 0030089545 Sterile individually wrapped 0030 089 642 0030089642 Biopur individually wrapped 0030 089 790 PCR clean Combitips advanced 2 5 mL 100 pieces 0030 089 448 0030089448 Eppendorf Quality 0030089553 Sterile individually wrapped 0030 089 650 0030089650 Biopur individually wrapped 0030 089 804 e PCR clean 54 Ordering Information Multipette M4 Repeater M4 English EN Order no Order no North Description International America Combitips advanced 5 0 mL 100 pieces 0030 089 456 0030089456 Eppendorf Quality 0030089561 Sterile individually wrapped 0030 089 669 0030089669 Biopur individually wrapped 0030 089 812 PCR clean Combitips advanced 10 mL 100 pieces 0030 089 464 0030089464 Eppendorf Quality 0030089570 Sterile individually wrapped 0030 089 677 0030089677 Biopur individually
10. out 5 Change the volume The display shows the possible dispensing steps 6 Dispense the liquid The step counter starts again at step 1 The display shows the dispensing steps that were carried out Operation Multipette M4 Repeater M4 31 English EN 5 3 2 Sequential step counter display for a Combitip that has been filled to capacity several times Prerequisites The Combitip is inserted Volume is set Liquid has been aspirated The reverse stroke has been carried out The step counter will not continue to count when the Combitip is only partially filled after it has been filled to capacity 1 Dispense the liquid The display shows the dispensing steps that have already been carried out 2 Aspirate the liquid again The display shows the possible dispensing steps 3 Carry out a reverse stroke The display shows the dispensing steps that have been carried out 4 Dispense the liquid The step counter continues to count the dispensing steps that have been carried out 32 Operation Multipette M4 Repeater M4 English EN 5 3 3 Resetting the step counter to Zero when Combitip is filled repeatedly Prerequisites The Combitip is inserted Volume is set Liquid has been aspirated The reverse stroke has been carried out The step counter will not continue to count when the Combitip is only partially filled after it has been filled to capacity 1 Dispense the liquid The display sh
11. step If there is not enough liquid for the selected dispensing volume the residual stroke lock will prevent any further dispensing operations No liquid will be dispensed when you press the operating lever After the residual stroke lock is activated you have 2 options Renewed aspiration a Slide the filling lever up to aspirate liquid b Press the operating lever to trigger the reverse stroke When the Combitip is partly filled you need to push the operating lever several times volume selection dial 4 c Continue dispensing When the Combitip advanced is filled completely the dispensing steps will continue to be counted A maximum of 999 dispensing steps can be counted Empty the Combitip advanced a Hold the Combitip advanced over the waste tube b To empty the Combitip advanced completely push the filling lever down as far as it will go If you now re fill the Combitip advanced or use a different Combitip advanced counting the dispensing steps will re start at 7 5 6 Ejecting the Combitip advanced To eject the Combitip it must be completely empty Proceed as follows 1 Start by pushing the filling lever down as far as it will go The display flashes On the display the Wsymbol appears steps MEL Operation Multipette M4 Repeater M4 37 English EN 2 Push the operating lever down The Combitip is ejected 3 Dispose of the Combitip advanced 5 6 1
12. with a maximum volume of 25 mL or 50 mL the Adapter advanced is also required D Dispenser A dispenser is a dosing device The dispenser dispenses the aspirated volume in steps Dispensing volume Volume per dispensing step E Eppendorf Quality Eppendorf Quality is an Eppendorf AG purity grade for consumables Eppendorf Quality meets the requirements for standard products e g precision accuracy wetting behavior and tightness Operating instructions Multipette M4 Repeater M4 9 English EN F Free jet dispensing Dispensing the liquid vvithout the Combitip touching the tube inner vvall Ifa drip forms on the Combitip after free jet dispensing this drip always belongs to the next dispensing step We recommend completing a dispensing series using only the free jet method or wall dispensing method Complete the reverse stroke in accordance with the dispensing series using either the free jet or wall dispensing method Errors listed in the technical data were determined using the wall dispensing method G Graduation Incremental graduation of a range a surface or a volume I Increment Step size or resolution ISO 8655 The ISO 8655 standard defines limiting values for systematic errors accuracy random errors precision and the test methods for dispensing devices M Maximum volume The maximum volume that can be used for dispensing N Nominal volume The maximum dispensing volume of a Combitip in c
13. wrapped 0030 089 820 PCR clean Combitips advanced 25 mL 100 pieces 4 Adapter 0030 089 472 0030089472 Eppendorf Quality 0030089588 Sterile individually vvrapped 0030 089 685 0030089685 Biopur individually vvrapped 0030 089 839 PCR clean Combitips advanced 50 mL 100 pieces 4 Adapter 0030 089 480 0030089480 Eppendorf Quality 0030089596 Sterile individually wrapped 0030 089 693 0030089693 Biopur individually wrapped 0030 089 847 PCR clean Order no Order no North Description International America Adapter advanced 25 mL 1 piece 0030 089 715 0030089715 Eppendorf Quality Adapter advanced 50 mL 1 piece 0030 089 723 0030089723 Eppendorf Quality Ordering Information Multipette M4 Repeater M4 55 English EN Order no Order no North Description International America Adapter advanced 25 mL 7 pieces 0030 089 731 0030089731 Biopur individually wrapped Adapter advanced 50 mL 7 pieces 0030 089 740 0030089740 Biopur individually wrapped 56 Ordering Information Multipette M4 Repeater M4 English EN 10 2 Accessories CombilongjCombitube The Combilong Combitube is an aspiration tool for the Combitips advanced It enables liquids to be directly taken out of all bottles Combitips advanced Rack A Combitips advanced Rack is available for storing the Combitips advanced lt 10 mL
14. CASO software to determine the maximum permissible systematic and random errors A Standard Operation Procedure SOP for the inspection is available on the CD Multipette M4 Repeater M4 and on our webpage Maintenance Multipette M4 Repeater M4 43 English EN 7 Maintenance 7 1 Cleaning NOTICE Damage to device from unsuitable cleaning fluids or sharp or pointed objects Unsuitable cleaning fluids can damage the device gt Never use corrosive cleaning fluids strong solvents or abrasive polishes Check the compatibility with the materials used Please note the information on chemical resistance see CD Do not clean the Multipette M4 Repeater M4 with acetone or organic solvents with a similar effect Do not clean theMultipette M4 Repeater M4 with sharp objects NOTICE Damage to the device due to penetration of liquids gt Do not allow any liquids to penetrate the inside of the housing If liquid has entered the inside of the housing the inner parts may only be repaired by Eppendorf AG service partners Contact your local sales office before returning any devices Proceed as follows K To remove any contamination on the outside dampen a soft cloth with a mild detergent and wipe the housing gt To disinfect the dispenser wipe the housing with isopropanol 70 K Rinse the Adapter advanced with demineralized water and dry K The Adapter advanced can be steam autoclaved at 121 C at
15. Combitip advanced 25 mL and 50 mL 1 Unscrew the reusable Adapter advanced from the Combitip advanced 2 If required rinse the Adapter advanced with demineralized water and dry O The Adapter advanced is a wear part Do not reuse the adapter if it shows any visible signs of wear An Adapter advanced is included in each box of 25 mL or 50 mL Combitips advanced 5 7 Dispensing with an empty battery The Multipette M4 Repeater M4 is also operational when the battery is empty Use the volume table to set the volumes see p 23 38 Troubleshooting Multipette M4 Repeater M4 English EN 6 Troubleshooting 6 1 6 1 1 Battery General errors Symptom message Cause Remedy The battery symbol Battery capacity is very K Replace the battery pom appears on the low display The battery symbol Battery capacity is K Replace the battery hp appears on the extremely low immediately display 6 1 2 Combitip advanced Symptom message Cause Remedy Combitip cannot be inserted No Combitip advanced used K Insert Combitip advanced Combitip cannot be filled No Combitip advanced used K Insert Combitip advanced Combitip cannot be ejected Filling lever not pushed down fully Operating lever not fully operated 1 Push the filling lever down as far as it will go 2 Operate the operating lever firmly and centered
16. L 45 uL 90 uL 225 uL 450 uL 0 2 mL 12 25 mL 4 5 mL 5 10 10pL 20 uL SOUL 100pL 1250 uL 500 uL 1 0mL 2 50 mL 5 0 mL e 9 11pL 22 uL 55 uL 1110 uL 1275 uL 550guL 11 1 mL 2 75 mL 5 5 mL 6 8 12pL 24gL 60 uL N20YL 1200 uL 600 uL 1 2mL 13 00 mL 6 0 mL e 7 13uL 26gL 65 uL N3ouL 325gL ob u 1 3 mL 3 25 mL 6 5 mL 7 7 yb 28 UL 70 uL 140uL 1250 uL 700 uL 1 4mL j3 50mL 7 0 mL e 6 15uL 20 UL 75 uL SOUL 375gL 750uL 1 5mL 3 75 mL 7 5 mL 8 6 16pL 22 UL 50 uL eu 1400 uL 800 uL 1 6mL 4 00mL 8 0 mL e 5 17pL B4pL Bu M70pL 425gL j850uL 11 7 mL 14 25 mL 8 5 mL 9 5 18uL 26 UL SOUL M80yL 450 ul 900 uL 1 8mL 14 50 mL 9 0 mL e 5 19pL 25 UL Spl 190uL 475uL 950uL 11 9 mL 475mL 9 5 mL 10 5 20uL 40 uL 100 pL 200 uL 500 uL 1000 pL 2 0 mL 5 00 mL 10 0 mL Operation Multipette M4 Repeater M4 25 English EN The following table shows different ways of dispensing 50 uL Desired Combitip advanced Number of Position of the dispensing dispensing steps volume selection volume when completely dial filled 50 uL 0 5 mL 10 5 color code purple 1 0 mL 20 Point between 2 and color code yellow 3 2 5 mL 50 1 color code green 5 0mL 100 First point color code blue Select a Combitip advanced according to the following criteria The desired dispensing volume is possible The desired number of dispensing steps is possible The geometry of the Combitip matches the geometry of the aspiration and target tubes
17. QZ j Storage vc tov be ccr ADI MADDEN EV 49 9 3 Disposal eene ehe e E Sele ease Rat Set 50 Ordering Information 51 10 1 Combitips advanced 53 10 2 ACCESSORIES Li sk emis he 56 Patentsz D ste re EEA eg C PIA dr 57 Operating instructions Multipette M4 Repeater M4 English EN 1 Operating instructions 1 1 Using this manual Read this operating manual completely before using the device for the first time Please also note the operating instructions for the accessories if applicable K The instructions for use for the Combitips advanced can be found on our webpage www eppendorf com cbt K This operating manual is part of the product Please keep it in a place that is easily accessible K Enclose this operating manual when transferring the device to third parties 1 2 Danger symbols and danger levels The safety precautions in these instructions have the following danger symbols and danger levels 1 2 1 Danger symbols Biohazard Explosion Toxic substances Hazard point Ej Material damage 1 2 2 Danger levels DANGER Will lead to severe injuries or death WARNING Can lead to severe injuries or death CAUTION Can lead to light to moderate injuries ATTENTION May lead to material damage Operating instructions Multipette M4 Repeater M4 7 English EN 1 3 Symbols used
18. TT ara ti on c CE ce H ace pron rme schi 6 C cc roduct named sehagigen grun AA G E ig fanart tid e Anwendung as Pd e CE ce e verli esti Ki t c Produkt 5d In tno case of ur funda E esten Andring e C e c lt 3 ce ce C bezeichn EE a nen Aderng des ce ce C Multi ung Pr n become rre G ltigk H C i c C ce ipette ias ris M T tte na 5 e C m er M4 49 W lt Pour c c s C ce C a ei Ki d o A KE e Kor ce CE ce e AS ce C E ex et TAS ce C qe SC Ki e C E Ee ES KEN ett cc A Pr e C ct e A ERE 2004 C c c CE C 108 EG LEC e E ce 2011 65 A A GEG EU AT ES v S DET E ce C qe E Kom K ct SES Ge AG ze C in ed e f E ce ce ce Vorstand q m a E G E qe C ce C ce CE pe 012 nt e C ze C Y SECH E Pn T3 e C ce c amp C c e C e U e CE c cc C m mE qe C PE e C e OU c CE ec Le C ET are ce vy lt b e C E ce C T K qe C 15 033506 0 ds c C e c ge C e ve 0 A ve C ce c ce ap OLEO CRE EK CA c 2 ee ce ce C T Ce ES ge C ce C ce KLE S GE pe CE e C Eve REED A ce E LK e CE qe C e AA A ue qe m eie bc ge C ce qe C ce e CE c KAT C c E gt L e LK eppendorf Evaluate your manual Give us your feedback www eppendorf com manualfeedback Your local distributor www eppendorf com worldwide Eppendo
19. an overpressure of 1 bar for 20 min The Adapter advanced can be autoclaved a maximum of 100 times K The Combitip rack advanced can be steam autoclaved at 121 C at an overpressure of 1 bar for 20 min The Combitip rack advanced can be autoclaved a maximum of 100 times Maintenance Multipette M4 Repeater M4 English EN 7 2 Replacing the battery When the display shows the battery symbol mm this indicates that the battery can be used for approx another 2 weeks When the display shows the battery symbol you need to replace the battery Proceed as follows 1 Remove the battery compartment lid N Grip the battery at the sides and remove Ww Hold the battery at the sides The symbol faces the user 4 Insert the battery at the top and then let it click into place at the bottom After electrical contact the display switches on briefly and all segments light up briefly 5 Close the battery compartment lid Maintenance Multipette M4 Repeater M4 45 English EN 7 3 Decontamination before shipment If you wish to return the device to Eppendorf AG or an Eppendorf AG service partner to be checked or repaired please note the following CAUTION Use of a contaminated device may result in personal injuries and damage to the device Clean and decontaminate the Multipette M4 Repeater M4 in accordance with the cleaning instructions before shipping or st
20. an select the dispensing volume before aspiration and change it during dispensing if required 5 2 1 Setting the volume before dispensing O Do not turn the selection dial beyond the lower or upper stop 1 Insert the required Combitip advanced 2 Push the filling lever down all the way 3 Turn the volume selection dial until it locks into the desired position The display shows the volume and the possible dispensing steps O The Multipette M4 Repeater M4 is equipped with automatic stroke shortening When you turn the selection dial the stroke of the operating lever is automatically set to the selected volume For a smaller volume the stroke will also be shorter When stroke shortening is active do not apply force to push the operating lever up 30 Operation Multipette M4 Repeater M4 English EN 5 3 Using the step counter On the display the step counter shows the dispensing steps next to steps The possible dispensing steps are displayed when the volume is selected During dispensing the dispensing steps that have been carried out are displayed 5 3 1 Step counter display for Combitip filled to capacity Prerequisites The Combitip is inserted 1 Set the volume 2 Aspirate the liquid The display will blink and display the possible dispensing steps 3 Carry out a reverse stroke The display shows 0 dispensing steps 4 Dispense the liquid The display shows the dispensing steps that were carried
21. e Multipette M4 Repeater M4 comply with the requirements of ISO 8655 part 5 Technical specifications subject to change 8 2 Ambient conditions Ambience Only for use indoors Ambient temperature 5 C 40 C Relative humidity 10 Vo 95 non condensing Atmospheric pressure 795 hPa 1060 hPa Transport storage and disposal Multipette M4 Repeater M4 49 English EN 9 Transport storage and disposal 9 1 Transport K Use the original packaging for transport Air temperature Relative humidity Atmospheric pressure General transport 25 C 60 C 10 Vo 95 Vo 300 hPa 1060 hPa Air freight 40 C 45 C 10 95 300 hPa 1060 hPa 9 2 Storage NOTICE Damage to device due to incorrect storage Remove the battery if you will not be using the Multipette M4 Repeater MA for longer periods of time Do not clean the Multipette M4 Repeater M4 while the Combitip is inserted Select a secure storage location Do not expose the Multipette M4 Repeater M4 to aggressive gases over a longer period of time NOTICE Damage due to UV radiation gt Do not store consumables in areas with strong UV radiation Air temperature Relative humidity Atmospheric pressure In transport 25 C 55 C 10 Vo 95 Vo 700 hPa packaging 1060 hPa Without transport 5 C 45 C 10 Vo 95 Vo 700 hPa packagin
22. ed and or autoclaved Combitips for dispensing NOTICE Damage to the device due to incorrect Combitip The dispenser locating is only suitable for Combitips advanced Other Combitips could damage the locating K Only use Combitips advanced 24 Operation Multipette M4 Repeater M4 English EN 5 1 1 Selecting the Combitip advanced The Multipette M4 Repeater M4 can only be used with Combitips advanced With the Multipette M4 Repeater M4 and each Combitip advanced you can select 20 different dispensing volumes K Use the volume table to select the corresponding volume and Combitip Selec Dis 0 1mL 0 2mL 0 5mL 1 0mL 2 5 mL 5 0mL 10mL 25mL 50 mL tion pens mm 9 ESE mm pm NEN dial jing white light purple yellow green blue orange red light steps blue gray e 100 M OpL 2 0 uL 50pgL MOULE 125 uL SOUL 0 1mL 0 25 mL 0 5 mL 1 50 2 0 uL 4OuL MOUL j20 SOUL 100 uL 0 2 mL 0 50 mL 1 0 mL e 33 2 0 UL nu Tu 20 uL 175 uL 150 uL 0 3 mL 0 75 mL 1 5 mL 2 25 40uL j8 0HL j20 L 40 uL 1100 uL 200 uL 0 4mL 1 00 mL 2 0 mL e 20 5 0 uL 110 uL 25ygL js0yi 125uL 250 uL 0 5mL 11 25 mLj2 5 mL 3 16 6 0 UL M2pL 30ygL 60 uL 150guL 300 uL 0 6mL 1 50 mLj3 0 mL e 14 7 0 uL 14 uL 25 uL joni 175uL 250 uL 0 7 mi 11 75 mL 3 5 mL 4 12 8 0uL jeu 40 uL 80 uL 200uL 400gL 0 8 mL 2 00 mL 4 0 mL e 11 9 0 UL M8p
23. enser Multipette M4 Repeater M4 The electrical supply for the electronics is via a battery The reliable detection and the secure seating during dispensing the Combitip advanced in the Multipette M4 Repeater M4 dispenser is ensured by a center sleeve in the Multipette M4 Repeater M4 dispenser The Combitip advanced is filled or emptied via the lower filling lever Dispensing is carried out with the upper operating lever If the piston in the Combitip advanced is pushed down as far as it will go with the filling lever the Combitip advanced can be ejected by pushing the operating lever The 25 mL or 50 mL Combitip advanced can only be used together with the corresponding Adapter advanced Combitip advanced and Adapter advanced are assembled before inserting into the Multipette M4 Repeater M4 dispenser All Combitip advanced and the two Adapter advanced are color coded Product description Multipette M4 Repeater M4 15 English EN 2 4 Combitip advanced Combitips advanced are single use items for aspirating and dispensing liquids according to the positive displacement principle Combitips advanced are used in Eppendorf dispensers e g Multipettes Repeaters and are made up of a cylinder and a piston Combitips advanced in sizes 25 mL and 50 mL require an Adapter advanced Combitips advanced are available in various color coded sizes 2 5 Overview of Combitips advanced with color codes 0 1 mL 0 2 mL 0 5 mL
24. er to insert the Combitip O If you want to have a different view of the text printed on the Combitip eject the Combitip and re insert it in a different position 1 Push the filling lever down 2 If the Combitip piston is pushed out carefully push it into the Combitip cylinder 3 Insert the Combitip straight from below Do not rotate the Combitip 4 If required push the filling lever down again The display shows the following information depending on the Combitip inserted and the position of the selection dial Operation 28 Multipette M4 Repeater M4 English EN The direction of the next piston movement A The selected dispensing volume 50 uL The maximum number of possible dispensing steps 50 steps stepes SD A rn LI pl 5 1 5 Picking up Combitips out of the rack 1 Push the Multipette M4 Repeater MA onto the Combitip 2 Push the filling lever down The display shows the following information depending on the Combitip inserted and the position of the selection dial The direction of the next piston movement A The selected dispensing volume 50 uL The maximum number of possible dispensing steps 50 steps steps AH A Du Operation Multipette M4 Repeater M4 29 English EN 5 2 Setting the volume The volume selection wheel has 20 positions Every second position is marked with a figure The other positions are marked with a dot You c
25. g 1060 hPa 50 Transport storage and disposal Multipette M4 Repeater M4 English EN 9 3 Disposal In case the product is to be disposed of the relevant legal regulations are to be observed Information on the disposal of electrical and electronic devices in the European Community Within the European Community the disposal of electrical devices is regulated by national regulations based on EU Directive 2002 96 EC pertaining to waste electrical and electronic equipment WEEE According to these regulations any devices supplied after August 13 2005 in the business to business sphere to which this product is assigned may no longer be disposed of in municipal or domestic waste They are marked with the following symbol to indicate this As disposal regulations may differ from country to country within the EU please contact your supplier if necessary E WARNING Risk of explosion and fire due to overheated accumulators and batteries K Do not heat accumulators and batteries to over 80 C and do not throw them into fires Do not dispose of accumulators and batteries as household waste Dispose of accumulators and batteries according to the locally applicable legal regulations Disposing of accumulators and batteries Ordering Information Multipette M4 Repeater M4 51 English EN 10 Ordering Information 10 0 1 Multipette M4 Repeater M4 Order no Order no North Descriptio
26. gical material of the risk group Il or higher please refer to the Laboratory Biosafety Manual source World Health Organisation Laboratory Biosafety Manual in its respectively current valid version WARNING Damage to health due to toxic radioactive or aggressive chemicals Wear personal protective equipment K Observe the national regulations for handling these substances K Observe the material safety data sheets and manufacturer s application notes Safety Multipette M4 Repeater M4 19 English EN CAUTION Danger to individuals due to careless use Never point the opening of a Multipette M4 Repeater M4 which is equipped with a Combitip at yourself or other persons K Only initiate dispensing if it is safe to do so With any dispensing task please ensure that you do not endanger yourself and other persons CAUTION Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety functioning and precision of the device Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment K Only use accessories and original spare parts recommended by Eppendorf NOTICE Carry over contamination and incorrect dispensing results due to the incorrect use of Combitips Combi
27. hook and loop tape and stick the hook and loop tapes to the back of the holder Press the hook and loop tapes down firmly 2 Clean glass or ceramic surfaces e g with ethanol and allow them to dry 3 Remove the protective film on the second hook and loop tape and press the hook and loop tape firmly against the clean surface Join the hook and loop tape on the Multipette M4 holder to the hoop and loop tape on the wall It may only be placed under load after 24 hours 22 Installation Multipette M4 Repeater M4 English EN 4 1 2 Inserting the holder into the pipette carousel You can hang the Multipette M4 Repeater M4 into a pipette carousel To hang the Multipette M4 Repeater M4 into the pipette carousel you need to insert the holder Proceed as follows 1 Undo the screw at the top end of the pipette carousel and remove it 2 Remove the cover 3 Press the pipette holder from below out of the mounting 4 Push the holder into the recesses in the pipette carousel from above 5 Close the cover 6 Tighten the screw Operation Multipette M4 Repeater M4 23 English EN Operation Inserting the Combitip advanced NOTICE Carry over contamination and incorrect dispensing results due to the incorrect use of Combitips Combitips are intended for single use Prolonged use can have a negative impact on dispensing accuracy K Only use Combitips once Do not use wash
28. is partially filled 32 5 4 Aspiratingliquid 33 KS Dispensing lIqUid cs ete qt ht er dde a 34 5 5 1 Discarding the reverse stroke 34 5 5 2 Dispensing liquid 35 5 6 Ejecting the Combitip advanced 36 5 6 1 Combitip advanced 25 mL and 50 mL 37 5 7 Dispensing with an empty battery 37 6 Troubleshooting 38 61 General errors yes era EE 38 6 1 1 Battery s vRlri Ae E le ee E 38 6 1 2 Combitip advanced 38 6 3 DISPIAV ss se sienne a Peete er e e 39 6 1 4 Error sle L rr dr nine E ves 40 6 1 5 Aspirating liquid 41 6 1 6 Measurement errors 42 Table of contents Multipette M4 Repeater M4 English EN Maintenance seede ee xus Eet det T ERT RR RT ai rd d 43 AAA CGleaning uuo x EE etat MN nes Se te Ems 43 7 2 Replacing the battery 44 7 3 Decontamination before shipment 45 Technical data sa an r s ae set Re ten ES t 46 8 1 Measurement errors 46 8 2 Ambient conditions 48 Transport storage and disposal 49 9 1 Transporta ns rado da OO d dd gd gs a dA 49
29. l reasons The Combitip is completely filled when the filling lever has reached the upper stop 4 Wipe off any outer wetting on the tip of the Combitip on the inner wall of the tube O To empty the Combitip you can push the filling lever down at any time 34 Operation Multipette M4 Repeater M4 English EN 5 5 Dispensing liquid 5 5 1 Discarding the reverse stroke During and after the liquid aspiration the display vvill blink After the liquid is aspirated the reverse stroke must be triggered Proceed as follovvs O Dispense the reverse stroke into the aspiration tube or a waste tube The reverse stroke is not a dispensing step Ifyou vvant to complete all dispensing steps using the wall dispensing method also complete the reverse stroke using the wall dispensing method f you want to complete all dispensing steps using the free jet dispensing method also complete the reverse stroke using the free jet dispensing method Ifa drip forms after free jet dispensing this drip always belongs to the next dispensing step When the Combitip is filled completely the operating lever needs to be pushed only once for the reverse stroke If the Combitip is partially filled you need to press the operating lever repeatedly if the selection dial setting is below 4 Position of the volume Number of times the operating lever was pressed selection dial for the reverse stroke e
30. lue Increment 40 uL 0 8 0 32 1 5 0 6 2 uL 0 5 mL 10 uL 0 9 0 09 1 5 0 15 purple Increment 100 uL 0 8 0 8 0 6 0 6 5 uL 1 mL 20 uL 0 9 40 18 40 9 40 18 yellovv Increment 200 uL 0 6 1 2 0 4 0 8 10 pL Technical data Multipette M4 Repeater M4 47 English EN Combitip advanced Testing volume Error limits Error Systematic error Random error uL HL 2 5 mL 50 uL 0 8 0 4 0 8 0 4 green Increment 500 uL 0 5 2 5 0 3 1 5 25 uL 5 mL 100 uL 0 6 0 6 0 6 0 6 blue Increment 1 000 uL 0 5 5 0 0 25 42 5 50 uL 10mL 200 uL 0 5 1 0 0 6 1 2 orange 0 2 mL Increment 2000 uL 0 5 10 0 25 5 0 0 1 mL 2 mL 25 mL 500 uL 10 4 2 0 0 6 3 0 red 0 5 mL Increment 5 000 uL 0 3 15 0 25 12 5 0 25 mL 5 mL 50 mL 1 000 uL 20 3 3 0 0 5 5 0 light gray 1 mL Increment 10 000 uL 0 3 30 0 3 30 0 5 mL 10 mL 48 Technical data Multipette M4 Repeater M4 English EN Test conditions and test evaluation in compliance with ISO 8655 Part 6 Test using a standardized fine balance with a moisture trap Number of determinations 10 Use of water in accordance with ISO 3696 Test was carried out with a Combitip advanced that was filled to capacity e Test was carried out at 20 C 27 C 0 5 C Dispensing onto the tube wall The test volumes for the systematic and random errors of th
31. n international America 4982 000 012 Multipette M4 4982000020 Repeater M4 Multipette M4 Starter Kit Multipette M4 Combitip Rack 4982 000 314 Combitip Assortmentpack Repeater M4 Starter Kit Repeater M4 Combitip Rack 4982000322 Combitip Assortmentpack 52 Ordering Information Multipette M4 Repeater M4 English EN 10 0 2 Accessories CAUTION Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety functioning and precision of the device Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment K Only use accessories and original spare parts recommended by Eppendorf Order no Order no North Description International America Pipette carousel 3115 with 6 pipette holder for Research 3115 000 003 022444905 and Reference Pipettes Holder 4982 602 004 4982602004 for Multipette M4 Hook and loop tape 4982 603 000 4982603000 for holder Battery 4980 215 003 022269119 3V Ordering Information Multipette M4 Repeater M4 53 English EN 10 1 Combitips advanced Order no Order no North Description International America Combitips advanced 0 1 mL 100 pieces 0030 089 405 0030089405 Eppendorf
32. onjunction with the selected dispensing device The term nominal volume comes from the ISO 8655 standard 10 Operating instructions Multipette M4 Repeater M4 English EN P PCR clean PCR clean is an Eppendorf AG purity grade for consumables PCR clean meets the requirements for standard products e g precision accuracy wetting behavior tightness PCR clean also meets the requirements with regard to absence of human DNA DNase RNase and PCR inhibitors Consumables with the PCR clean purity grade are controlled and certified by an external laboratory Certificates are available for downloading from our webpage www eppendorf com Positive displacement principle The liquid comes into direct contact with the Combitip piston during aspiration and dispensing Unlike with a pipette the liquid and piston are not separated by an air cushion A small air bubble is visible at the piston during dispensing R Random error Precision Standard deviation of the average value of the dispensed volumes Remaining stroke Liquid reserve The liquid which remains after all dispensing steps have been completed You can discard the liquid of the remaining stroke or reuse it Residual stroke lock The residual stroke lock prevents that the incorrect volume is being dispensed if there is not enough liquid available for the dispensing volume Reverse stroke After aspiration the piston is moved into a defined position Liquid is dis
33. orage Hazardous substances are solutions presenting a hazard to health potentially infectious agents organic solvents and reagents radioactive substances proteins presenting a hazard to health DNA 1 Please note the information in the document Decontamination certificate for product returns You can find it as a PDF file on our webpage www eppendorf com The decontamination certificate can also be found on the enclosed CD 2 Enter the serial number of the Multipette M4 Repeater M4in the decontamination certificate 3 With the shipment please include the completed decontamination certificate for product returns with the Multipette M4 Repeater M4 4 Send the pipette to Eppendorf AG or an Eppendorf AG service partner The address details of Eppendorf AG can be found on the last page of this operating manual The list of service partners can be found at www eppendorf com worldwide 46 Technical data Multipette M4 Repeater M4 English EN 8 Technical data Multipette M4 Repeater M4 VVeight 105g Battery Type Button cell Voltage 3V Operational life approx 2 years 8 1 Measurement errors Combitip advanced Testing volume Error limits Error Systematic error Random error Din HL HL 0 1 mL 2 uL 1 6 0 032 3 0 x 0 06 white Increment 20 uL 1 0 0 2 2 0 0 4 1 uL 0 2 mL 4 uL 1 3 0 052 2 0 0 08 light b
34. ows the dispensing steps that were carried out 2 Push the filling lever down 3 Discard the remaining volume The display flashes 4 Aspirate the liquid again The display shows the possible dispensing steps 5 Carry out a reverse stroke The display shows 0 dispensing steps 6 Dispense the liquid The display shows the dispensing steps that were carried out 5 3 4 Step counter display if Combitip is partially filled Prerequisites The Combitip is inserted 1 Set the volume 2 Aspirate part of the liquid The display flashes 3 Carry out a reverse stroke The display shows 0 dispensing steps 4 Dispense the liquid The display shows the dispensing steps that were carried out The step counter will also continue to count when the lowest position residual stroke lock of the filling lever is reached Operation Multipette M4 Repeater M4 33 English EN 5 4 Aspirating liquid If you are aspirating solutions with a high viscosity into a Combitip with a large volume draw up the filling lever especially slowly This prevents any leakage occurring between the piston and the cylinder in the Combitip Proceed as follows 1 To ensure that the Combitip is in its basic position push the filling lever down 2 Immerse the tip of the Combitip in the liquid 3 Slowly and steadily slide the filling lever up The display flashes during aspiration The small air bubble at the Combitip piston is due to technica
35. pensed during this piston movement The reverse stroke is not a dispensing step Operating instructions Multipette M4 Repeater M4 11 English EN S Sterile Sterile is an Eppendorf AG purity grade for consumables Sterile meets the requirements for standard products e g precision accuracy wetting behavior tightness Sterile also meets the requirements with regard to sterility and freedom from pyrogens Stroke A stroke is the distance travelled by the piston Systematic error Accuracy Deviation of the average value of the dispensed volumes from the selected volume Product description 12 Multipette M4 Repeater M4 English EN 2 2 1 Product description Main illustration 1 Volume selection dial 2 Position indicator 3 Display 4 Operating lever 5 Filling lever 6 Serial number 7 Finger rest 8 Battery compartment lid Product description Multipette M4 Repeater M4 13 English EN 2 2 Display When you insert a Combitip the display switches on automatically The displays switches off automatically when it has not been used for a period of time Sleep function The display automatically switches back on when the Multipette M4 Repeater M4 is moved If no Combitip is inserted the display does not switch back on when moved dE steps Ge US C H d ALILI uk 1 Number of steps 3 Possible direction of the next When selecting the volume
36. pensing 34 35 Free jet dispensing 35 Reverse Stroke AEN 34 Wall dispensing 35 M Material reasda rai ni 16 Measurement errors sessen 46 R Reverse stroke 34 S Selection dial settings 34 Sleep function Display evene 13 Step counter ain nananshan 30 SS 30 steps Step counte Pysen ia 30 Storage diese 49 T Technical data Ambient conditions 48 Battery for M4 46 Multipette M4 Repeater MA 46 Troubleshooting Aspirating liquid 41 Batten iet 38 Combitip advanced 38 Display x ees 39 Error codes teo cn 40 Measurement errors 42 V Volume DETIN Ge viseve eine tee gei 29 Volume Lable eee 24 W Wall dispensing 35 Warranty siepi ini 17 Index Multipette M4 Repeater M4 59 English EN LE SEKR C ce C CE Sy LE ee C e CE CE e EE AE e CENE Le AS ee C qe C E C Ce c C C C ee e qe CE C e E amp S KG E Ke E ECKER ect ce EG K e C kek ke te E C re C gd zg KS onf qe C e C e C LES qe C C ormi ce C ce Co it c K e K v Arr T nformi atse Les A E c C t c CE c M mity rkl ru ee C cc C ge C E er c Mio e een Decl ng e ME AE das p s Rron und Nor
37. rf AG Hamburg Germany Phone 49 40 538 01 0 www eppendorf com
38. rial Cylinder Polypropylene PP Piston 0 1 mL and 0 2 mL Polyethylene PE with glass fiber GF Piston 0 5 mL to 50 mL Polyethylene PE Adapter advanced Polybutylene terephthalate PBT 2 8 Warranty Please contact your local Eppendorf contractual partner for warranty claims No warranty is given in the following cases n the case of misuse If unauthorized persons open the dispenser The following assemblies are excluded from the warranty Wear parts Battery Safety Multipette M4 Repeater M4 English EN 3 Safety 3 1 Intended use The Multipette M4 Repeater M4 is a lab device and in combination with a Combitip advanced it is intended for dispensing aqueous solutions in the volume range of 1 uL 10 mL In vivo applications applications in or on the human body are not permitted The Multipette M4 Repeater M4 may only be operated by trained specialists All users must have read the operating manual carefully and familiarized themselves with the device s mode of operation 3 2 Warnings for intended use WARNING Damages to health due to infectious liquids and pathogenic germs When handling infectious liquids and pathogenic germs observe the national regulations the biological security level of your laboratory the material safety data sheets and the manufacturer s application notes Wear personal protective equipment For comprehensive regulations about handling germs or biolo
39. tip advanced or on the advanced Adapter advanced faulty S03 Err e Volume selection dial not Let the number or point engaged engage exactly above the position display Troubleshooting Multipette M4 Repeater M4 41 English EN 6 1 5 Aspirating liquid Symptom message Cause Remedy Large air bubble in Air has been aspirated gt Re aspirate the liquid Combitip advanced while aspirating the after the liquid has liquid been aspirated Highly viscous liquid has Aspirate liquid more been aspirated too slowly quickly e Lag time of the liquid not gt Aspirate liquid more observed slowly 42 Troubleshooting Multipette M4 Repeater M4 English EN 6 1 6 Measurement errors Symptom message Cause Remedy Systematic and or e Reverse stroke given as gt Repeat dispensing random error is too dispensing volume by high mistake Operating lever not fully Repeat dispensing pushed during dispensing Combitip advanced used Repeat dispensing too many times too old Not enough liquid was gt Repeat dispensing aspirated e Many air bubbles in the Repeat dispensing aspirated liquid Combitip held at an incorrect angle during dispensing Regularly check the precision and accuracy of the Multipette M4 Repeater M4 with the Combitips advanced lots you are using to prevent dispensing errors You can use the PI
40. tips are intended for single use Prolonged use can have a negative impact on dispensing accuracy Only use Combitips once Do not use washed and or autoclaved Combitips for dispensing NOTICE Damage to the device due to penetration of liquids gt Do not allow any liquids to penetrate the inside of the housing If liquid has entered the inside of the housing the inner parts may only be repaired by Eppendorf AG service partners Contact your local sales office before returning any devices 20 Safety Multipette M4 Repeater M4 English EN 3 3 Information on product liability In the following cases the designated protection of the device may be compromised Liability for any resulting property damage or personal injury is then transferred to the operator The device is not used in accordance with the operating manual The device is used outside of its intended use The device is used with accessories or consumables which are not recommended by Eppendorf The device is maintained or repaired by people not authorized by Eppendorf The user makes unauthorized changes to the device Installation Multipette M4 Repeater M4 21 English EN 4 Installation 4 1 Using the holder The accessories include a holder for the Multipette M4 Repeater M4 The holder can be used for wall mounting or as a holder in the pipette carousel 4 1 1 Mounting the holder on a wall UA 1 Remove the protective film on a
41. www eppendorf com myeppendorf SQ Operating manual Copyright O 2013 Eppendorf AG Hamburg No part of this publication may be reproduced without the prior permission of the copyright owner Trademarks Eppendorf and the Eppendorf logo and are registered trademarks of Eppendorf AG Hamburg Germany eppendorf Multipette9 Repeater9 Combitips advanced9 and Biopur are registered trademarks of Eppendorf AG Hamburg Germany Registered trademarks are not marked in all cases with TM or 9 in this manual 4982 900 099 01 032013 Table of contents Multipette M4 Repeater M4 3 English EN Table of contents 1 Operating instructions 6 1 1 Using this manuali e ON 2k TAS 6 1 2 Danger symbols and danger levels 6 1 2 1 Danger symbols 6 1 2 2 Danger levels 6 1 53 Symbols used ii le se RS Lieder ves eus 7 L4 GIOSSARY is De AAA pa nd ERR UOS 7 2 Product descripti n iei eee da 12 2 1 Main illustration 12 2 2 Display ee eee e DEUS RE ERN ee 13 2 3 Features aid os eis DII UP teh PeeD d EN eut 14 24 Combitipadvanced cer i pee ee 15 2 5 Overview of Combitips advanced with color codes 15 2 6 Delivery package 3 152288 x mE Men cR Rep ans 16 2 4 Materials i wads eds de kc edis d BRA Re 16 2 87 Waranty vs i EE LIRE
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