Home

"user manual"

Contents

1. SSV cable Cover sheet Screws Solvent selection valve 172 1260 Binary Pump User Manual Maintenance 10 In tion of t it 3 Install the Solvent selection assembly by fixing both 4 Connect the outlet for solvent A upper row to the inlet indicated screws valve of the left pump head Connect the outlet for solvent B lower row to the inlet valve of the right pump head Put the solvent bottles into the solvent cabinet Connect the bottle heads of solvents A1 and A2 to the inlets in the upper row see labels on valve assembly Connect the bottle heads of solvents B1 and B2 to the inlets in the lower row see labels on valve assembly To solvent bottles Inlets Outlets For controlling the solvent selection valve please refer to the online help or user manual of your control software 1260 Binary Pump User Manual 173 10 Maintenance Exchanging the Solvent Selection Valve When If leaking internally croossflow between the ports or if one of the channels is blocked Tools required Screwdriver Pozidriv 1 Parts required Description G1381 60000 Solvent Selection Valve Upgrade Kit Preparations Switch off the pump at the main power switch 1 Lift solvent bottles out of the solvent cabinet and place 2 Disconnect all tubings from the solvent selection valve them on the table Disconnect the solvent tubes from the 7 solvent se
2. 76 1260 Binary Pump User Manual Using the Pump 4 Setting up the Pump with Agilent ChemStation Pump Auxiliary Parameters The parameters in this panel are pre set to fit most applications Adjustments should only be made when required The Pump Auxiliary panel can be accessed by the menu via Instrument gt More Pump gt Auxilaryor by left clicking the pump icon in the GUI Pump Auxiliary M Branne se A bar Breinnial AG bar Figure 21 Pump Auxiliary Parameter Panel 1260 Binary Pump User Manual 77 4 Using the Pump Table 17 Parameters of the Pump Auxiliary Panel Parameter Limits Description Maximum Flow Gradient Minimum Stroke Compressibility 0 1 100 mL min default 100 mL min 20 uL 100 uL default Auto 0 150 10 Sbar or enhanced compressibility calibration default use enhanced comp calibration With this parameter flow rate changes can be ramped up and down slowly to avoid pressure shocks to the column The default value is 100 ml min which in fact turns the function off Caution The flow is shut off immediately when the pump switched to standby When the pump is turned On from the Off status the pump drive initializes thereby ignoring the maximum flow gradient setting Depending on system delay volume and flow restriction the system pressure may rise very quickly to a high value To protect your column from damage it is suggested to open the pu
3. Agilent Technologies Notices Agilent Technologies Inc 2005 2008 2010 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61312 90012 Edition 06 10 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate
4. Figure 42 Active Inlet Valve Assembly 198 1260 Binary Pump User Manual Parts and Materials for Maintenance 11 Accessory Kit Accessory Kit p n G1312 68755 p n Description 0890 1195 Drain tube 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 5181 1519 CAN cable Agilent module to module 1 m 5988 8453EN Capillary fitting starter kit brochure 9222 0519 Bag plastic G1312 87303 SS Capillary 400 x 0 17 mm m m ps ps G1312 87304 SST capillary 700 mm 0 17 mm i d 1 32 1 32 01200 60001 RRLC system configurator A 01 01 CD ROM G1311 90107 Algae note 5042 9954 4x Tubing clip 2x re order 4 pk G1311 60003 Bottle head assembly 2x 1260 Binary Pump User Manual 199 11 Parts and Materials for Maintenance Accessory Kit Accessory Kit p n G1312 68765 p n Description 0890 1195 Drain tube 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 5181 1519 CAN cable Agilent module to module 1 m 5988 8453EN Capillary fitting starter kit brochure 9222 0519 Bag plastic G1312 87303 SS Capillary 400 x 0 17 mm m m ps ps G1312 87304 SST capillary 700 mm 0 17 mm i d 1 32 1 32 01200 60001 RRLC system configurator A 01 01 CD ROM G1311 90107 Algae note 5042 9954 4x Tubing clip 2x re order 4 pk G1311 60003 Bottle head assembly 4x 200 1260 Binary Pump User Manual Parts and Materials for Maintenance 11 Active Seal Wash Option Active Seal Wash Option kit p n G1
5. 122 1260 Binary Pump User Manual Troubleshooting and Diagnostics 7 Elasticity Calibration Various parts in the flow path of the binary pump have a certain elasticity which needs to be compensated to obtain the lowest pressure flow and composition ripple possible This is done by running an elasticity calibration after maintenance and major repairs For details see Pump Elasticity Calibration on page 138 Diagnostic Signals The pump has several signals pressure voltages and piston movement that can be used for diagnosing pressure stability composition and flow problems see 1260 Binary Pump User Manual 123 7 Troubleshooting and Diagnostics dicators Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status Indicator Power switch Serial number Figure 25 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 124 1260 Binary Pump User Manual Troubleshooting and Diagnostics 7 Module Status Indicator The module status indicator indicates one of six possible module conditions e When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis e A
6. 218 1260 Binary Pump User Manual Identifying Cables 12 RS 232 Cables p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1260 Binary Pump User Manual 219 12 Identifying Cables Agilent 1200 Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control Module 220 1260 Binary Pump User Manual This appendix provides general safety and environmental information 1260 Binary Pump User Manual 13 Appendix General Safety Information 222 The Waste Electrical and Electronic Equipment Directive 225 Lithium Batteries Information 226 Radio Interference 227 Sound Emission 228 Solvent Information 229 Agilent Technologies on Internet 230 ee Agilent Technologies 221 13 Appendix General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design m
7. external contact cable 218 F fan failed 106 features instrument layout 8 filter solvent inlet 70 firmware updates 183 183 upgade downgrade 183 upgrade downgrade 183 flow accuracy 42 flow connections 58 61 flow precision 42 flowrange 8 operational 42 setable 42 flow rate minimum 70 frequency range 41 G gas solubility 70 gradient formation 42 hexagonal key 3mm 156 162 165 hexagonal key 3mm 163 hexagonal key 4mm 156 162 165 hexagonal key 4mm 163 high pressure mixing 8 hints for successful use 70 humidity 41 hydraulic path with solvent selection valve 188 hydraulic path without solvent selection valve 190 hydraulic system 42 index limit 117 index adjustment 118 index missing 118 initialization failed 119 initialization 15 injection precision 88 inlet valve body 153 inlet valve 167 1260 Binary Pump User Manual Index inlet valve fuse 112 inlet valve missing 115 inserttool 163 installation bench space 40 instrument layout 21 interface board 182 interface cable 5 7 interfaces 24 internet 230 introduction to the pump 8 L LAN cable 217 leak sensor open 104 leak sensor short 105 leak 104 line frequency 41 line voltage 41 liquimeter 82 144 160 lithium batteries 226 M main assemblies overview 151 maintenance procedures 144 maintenance procedures 82 maintenance feedback 20 replacing firmware 183 183 message ignition without cover 107 107 remote timeout
8. Degasser Pump HiPALS 0 12 mm seat Cooler for ALS TCC DAD SL 50 This setup is used for best Signal to noise ratio using 2 1 mm and 3 0 mm columns For a more detailed help on configuring your instrument refer to the RRLC system configurator A 01 01 CD ROM p n 01200 60001 400 mm x 0 17 mm ca 320 mm x 0 12 mm amp K amp d d New LowDelayVolmne Heat Exchanger New LDV post column cooler 0 12 mmID cap 2 ul cell Figure 8 1200 RRLC system in low delay volume configuration for 2 1 mm amp 3 0 mm id columns 1260 Binary Pump User Manual Installing the Pump 3 Optimizing the Stack Configuration 1260 Binary Pump User Manual 51 3 Installing the Pump Optimizing the Stack Configuration 1200 RRLC in Low Delay Volume Configuration Degasser Pump HiPALS 0 12 mm seat Cooler for ALS TCC DAD SL 52 In this configuration the RRLC is optimized for speed with the 2 1 mm columns For a more detailed help on configuring your instrument refer to the RRLC system configurator A 01 01 CD ROM p n 01200 60001 400 mm x 0 17 mm ca 320 mm x 0 12 mm amp K amp d d New LowDelayVolmne Heat Exchanger New LDV post column cooler 0 12 mmID cap 2 ul cell Figure 10 1200 RRLC system in low delay volume configuration for 2 1 mm amp 3 0 mm id columns 1260 Binary Pump User Manual Installing the Pump 3 Optim S C i t j the St Configuration 12
9. Figure 35 Exchanging the Interface Board 182 1260 Binary Pump User Manual Maintenance 10 Replacing Module Firmware When Tools required Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third part control software requires a special version LAN RS 232 Firmware Update Tool or Agilent Diagnostic Software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 To load the firmware into the module follow the instructions in the documentation 1260 Binary Pump User Manual 183 10 Maintenance 184 Module Specific Information Table 25 Module Specific Information G1312B G1312B Binary pump SL Initial firmware A 06 01 Compatibility with 1100 1200 When using the G1312B ina system all ot
10. This would lead into invalid and not portable solvent compressibility data gt Make sure to perform accurate pump elasticity calibration Make absolutely sure that the pump is very thoroughly flushed with the solvent to be calibrated before starting the procedure Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the calibration to fail 1 Select the solvent from the test selection menu 2 Start the test and follow the instructions Make sure to release the pressure by opening the purge valve when the test has finished completed Otherwise the pump may generate an overpressure error 1260 Binary Pump User Manual 137 8 Tests Functions and Calibration Pump Elasticity Calibration CAUTION Description The flow path components of the Binary Pump SL pump have an inherent and pressure dependent elasticity which differs from pump to pump When this elasticity pressure function is known a correction algorithm can be applied This results in significantly improved pump performance in low delay volume mode damper and mixer bypassed The pump elasticity calibration uses a solvent with well known properties HPLC grade water to determine the pump elasticity over the entire operating pressure range and stores the calibration values in the non volatile RAM of the pump mainboard The initial calibration is done at the factory It only needs to be repeated after replacement of major pu
11. and repeat the pressure test Exclude each module one by one to determine which module is leaking Ifthe pump is determined to be the source of the leak run the pump test to identify the defective pump component 1260 Binary Pump User Manual Tests Functions and Calibration 8 Potential Causes of Pressure Test Failure After isolating and fixing the cause of the leak repeat the pressure test to confirm the system is pressure tight Table 20 Potential Cause Pump Potential Cause Pump Corrective Action Purge valve open Close the purge valve Loose or leaky fitting Tighten the fitting or exchange the capillary Damaged pump seals or plungers Run the pump test to identify the defective component Loose purge valve Tighten the purge valve nut 14 mm wrench Table 21 Potential Cause Autosampler Potential Cause Autosampler Corrective Action Loose or leaky fitting Tighten or exchange the fitting or capillary Rotor seal injection valve Exchange the rotor seal Damaged metering seal or plunger Exchange the metering seal Check the plunger for scratches Exchange the plunger if required Needle seat Exchange the needle seat Table 22 Potential Cause Column Compartment Potential Cause Column Compartment Corrective Action Loose or leaky fitting Tighten or exchange the fitting or capillary Rotor seal column switching valve Exchange the rotor seal 1260 Binary Pump User M
12. p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module 1 1 BCD 5 20 2 2 BCD7 80 8e 7 3 3 BCD6 40 4 4 BCD 4 10 5 5 BCDO 1 1e 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 216 1260 Binary Pump User Manual CAN LAN Cables Identifying Cables 12 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 1260 Binary Pump User Manual Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection 217 12 Identifying Cables External Contact Cable 5o C G 1 100 lt 6 ie e e11 One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent Module Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 a Brown 2 EXT 1 x Green 3 EXT 2 Bea Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected
13. 13 Appendix Safety Symbols Table 26 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the A instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced G by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up WARNING es alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 224 1260 Binary Pump User Manual Appendix 13 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates tha
14. 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1260 Binary Pump User Manual 209 12 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog E57 210 1260 Binary Pump User Manual Identifying Cables 12 Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module HES AAA Shield Shield Analog ft E pi Q i Center Center Analog Agilent Module to General Purpose p n 01046 60105 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Black Analog 3 Red Analog E xg 1260 Binary Pump User Manual 211 12 Identifying Cables Remote Cables 212 O O JNE One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be
15. Comment method printouts etc Timetable max number of lines Use the timetable to build solvent gradients flow gradients or combinations of depends on free space both Gradients are always linear Use multiple timetable entries to mimic in pump memory exponential or parabolic gradients Display There are three ways to display the timetable e in tabular form as flow pressure graph as solvent percentage plot Values can only be changed in tabular view 74 1260 Binary Pump User Manual Using the Pump 4 Pump Control The Pump Control panel is used to turn the pump on and off operate the optional seal wash pump and define an error method CAUTION Upon initialization the pump ignores the Maximum Flow Gradient value see Table 17 on page 78 This can result in a rapid and uncontrolled pressure increase gt To prevent harm to the column open the purge valve until the initialization is finished 1260 Binary Pump User Manual 75 4 Using the Pump Setting up the Pump with Agilent ChemStation 1 Open menu Instrument gt More Pump gt Control or click on the pump icon in the GUI Pump Control Sea Westar Oris SP wie fe Single Was Duration itt f Penance arle iia Wha For Tle 1 9 2006 15 32 31 Figure 20 Pump Control Panel The pump group enables you to switch the pump On Off or to Standby In Standby the pump motor is still energized When the pump is switched on again it does not re initialize
16. Prevent blocking of solvent inlet filters never use the pump without solvent inlet filters Growth of algae should be avoided see Prevent Blocking of Solvent Filters on page 86 Check purge valve frit and column frit in regular time intervals A blocked purge valve frit can be identified by black yellow or greenish layers on its surface or by a pressure greater than 10 bar when pumping distilled water at a rate of 5 mL min with an open purge valve Whenever possible use a minimum flow rate of 5 uL min per solvent channel to avoid crossflow of solvent into the unused pump channel Whenever exchanging the pump seals the purge valve frit should be exchanged too When using buffer solutions flush the system with water before switching it off The seal wash option should be used when buffer solutions with concentrations of 0 1 M or higher are being pumped for long periods of time Check the pump plungers for scratches grooves and dents when changing the piston seals Damaged plungers cause micro leaks and will decrease the lifetime of the seals 1260 Binary Pump User Manual Using the Pump 4 e After changing the plunger seals apply the seal wear in procedure see Exchanging the Pump Seals on page 159 e Place the aqueous solvent on channel A and the organic solvent on channel B The default compressibility settings are set accordingly 1260 Binary Pump User Manual 71 4 Using the Pump Setting up the Pump with
17. Serial Number Information ALL 23 Rear view of the module 23 Interfaces 24 Overview Interfaces 26 Setting the 8 bit Configuration Switch 30 Communication Settings for RS 232C 33 Special Settings 35 This chapter gives an introduction to the module instrument overview and internal connectors ee Agilent Technologies 1 Introduction to the Binary Pump Instrument and Operation Instrument Layout The Binary Pump comprises two identical pumps integrated into one housing Binary gradients are created by high pressure mixing An optional degasser is available for applications that require best flow stability especially at low flow rates for maximum detector sensitivity Pulse damper and mixer can be bypassed for low flowrate applications or whenever a minimal transient volume is desirable Typical applications are high throughput methods with fast gradients on high resolution 2 1 mm columns The pump is capable of delivering flow in the range of 0 1 5 mL min against up to 600 bar A solvent selection valve optional allows to form binary mixtures isocratic or gradient from one of two solvents per channel Active seal wash optional is available for use with concentrated buffer solutions The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP foam plas
18. Then the sequence starts again moving the pistons up and down between the two limits During the delivery stroke of the first piston the solvent in the pump head is pressed through the outlet ball valve into the second pumping unit The second piston draws in half of the volume displaced by the first piston and the remaining half volume is directly delivered into the system During the drawing stroke of the first piston the second piston delivers the drawn volume into the system 1260 Binary Pump User Manual 15 1 16 Introduction to the Binary Pump Delay volume From mixing point to pump outlet dependent on back pressure 120 pL without damper and mixer 600 800 pL with damper and mixer Materials in contact with mobile phase Pump head SST gold sapphire ceramic Active inlet valve SST sapphire ruby ceramic PTFE Outlet valve SST gold sapphire ruby tantalum Adapter SST gold Purge valve SST gold PTFE ceramic Damping unit Gold SST For pump specifications see Performance Specifications on page 42 What is Pump Elasticity Compensation The flow path of the pump consists of pump chambers sapphire pistons polymer seals stainless steel tubing of different dimension pressure sensor and so forth All of these parts deform when pressurized The sum of this deformation is called pump elasticity Let us look at a practical example Piston 1 draws solvent at ambient pressure The movement direction is
19. 103 minimum flow rate 70 missing pressure reading 111 missing parts 47 mixer 9 when to remove 92 1260 Binary Pump User Manual motor drive power 114 Non operating altitude 41 non operating temperature 41 0 operating Altitude 41 operating temperature 41 153 169 196 overpressure error 131 135 137 139 outlet valve P packaging damaged 46 parts identification cables 207 parts active inlet valve 198 bottle head assembly 186 damaged 47 hydraulic path with solvent selection valve 188 hydraulic path without solvent selection valve 190 missing 47 outlet valve 196 performance specifications 42 pHrange 42 physical specifications 41 piston 14 152 162 plunger 14 power supply indicator 124 power consideration 38 power consumption 41 power cords 39 power switch 56 pressure above upper limit 109 pressure below lower limit 109 pressure pulsation 13 pressure range 90 pressure pulsation 18 96 pressure ripple 136 pressure test results 135 pressure operating range 42 pulsation 42 priming with a pump 66 PTFE frit 153 153 pulsation pressure 13 18 ump elasticity calibration 139 ump head missing 117 O v pump seals for normal phase solvents 90 pump stroke AUTO mode 13 pump seals alternative material 90 purge valve 70 153 153 radio interference 227 reassembling the pump head 165 recommended pH range 42 remote cable 212 remove damper and mixer 92 removing the
20. 32 17 5062 2461 Waste tube 5 m reorder pack 188 1260 Binary Pump User Manual Parts and Materials for Maintenance 11 Hydraulic Path with Solvent Selection Valve an Figure 37 Hydraulic Path with Solvent Selection Valve 1260 Binary Pump User Manual 189 11 Parts and Materials for Maintenance Hydraulic Path without Solvent Selection Valve Item p n Description 1 G1322 67300 Kit of 4 solvent tubes for connection degasser to SSV including labels 2 0100 1847 Adapter AIV to solvent inlet tubes G1312 60066 Passive inlet valve 3 G1312 60025 Active inlet valve body optional without cartridge 4 G1312 60064 Pump Head without Seal Wash 5 G1312 60067 Outlet valve complete 6 G1312 87300 Absorber capillary 7 G1312 67302 Mixing capillary 8 G1312 87301 Restriction capillary mixing capillary to pressure sensor 9 5067 1527 Pressure sensor 10 G1312 87305 Capillary SSL 0 17 x 150 mm pressure sensor to damper 11 G1312 60031 Damper 12 G1312 87330 Mixer 13 G1312 87306 Capillary SSL 0 17 x 105 mm connections to solvent mixer G1312 04100 Bracket for solvent mixer 14 G1312 60061 Purge valve assembly 15 G1312 87303 SS Capillary 400 x 0 17 mm m m ps ps G1312 87304 SST capillary 700 mm 0 17 mm i d 1 32 1 32 16 5062 2461 Waste tube 5 m reorder pack 17 5042 8507 Seal wash pump cartridge silicone tubing 18 5065 9978 Tubing 1 mm i d 3 mm o d silicone 5 m for seal wash option 190 1260 Binary P
21. 8 Tests Functions and Calibration Pressure Test Description 130 Positioning the Blank Nut 131 Running the Pressure Test 131 Evaluating the Results 132 Pump Test 134 Running the Pump Test 135 Evaluating the Results 135 Binary Pump Solvent Calibration 136 Running the Solvent Compressibility Calibration 137 Pump Elasticity Calibration 138 Running the Pump Elasticity Calibration 139 This chapter explains all test functions that are available for the binary pump te Agilent Technologies 129 8 Tests Functions and Calibration Pressure Test Description CAUTION Description The pressure test is a quick built in test designed to demonstrate the leak tightness of the system The test involves monitoring the flow profile while the pump delivers against a blank nut The result is presented as the leak rate of the module and provides information about the leak tightness of the system between the outlet ball valves of the pump and the blank nut The blank nut can be positioned anywhere between the purge valve of the pump and the detector inlet to pressure test the desired part of the system Blank nut placed at the outlet of flow cell The applied pressure may cause permanent leaks or bursting of the flow cell gt Never include the flow cell in the pressure test 130 Step 1 The test begins with the initialization of both pump heads After initialization the pump is starting the compression phase and the required flow r
22. AS G1364D FC uS G1367E HiP ALS G1377A HiP micro ALS G2258A DL ALS G4226A ALS Detectors G1314B VWD VL G1314C VWD VL G1314E F VWD Yes No Yes Yes Yes Yes Yes No No Yes No No No No No Yes Yes Yes Yes Yes Yes Yes Yes Yes No No No No No Yes Yes Yes Yes Yes Yes Yes Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330B THERMOSTAT for G1330B CAN DC OUT for CAN slaves 24 1260 Binary Pump User Manual Introduction to the Binary Pump 1 Table 2 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4212A B DAD 2 No Yes Yes 1 Yes G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 1 Yes G1362A RID G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1316A C TCC 2 No No Yes No Yes G1322A DEG No No No No No Yes AUX G1379B DEG No No No Yes No No AUX G4227A Flex Cube 2 No No No No No G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software RS 232C as interface to a computer e REMOTE connector as in
23. Binary Pump User Manual Introduction to the Binary Pump 1 Table 1 Pump Details Delay volume From mixing point to pump outlet dependent on back pressure 120 pL without damper and mixer 600 800 uL with damper and mixer Materials in contact with mobile phase Pump head SST gold sapphire ceramic Active inlet valve SST sapphire ruby ceramic PTFE Outlet valve SST gold sapphire ruby tantalum Adapter SST gold Purge valve SST gold PTFE ceramic Damping unit Gold SST For pump specifications see Performance Specifications on page 42 1260 Binary Pump User Manual 11 1 12 Introduction to the Binary Pump Optimization Features What is Pump Elasticity Compensation The flow path of the pump consists of pump chambers sapphire pistons polymer seals stainless steel tubing of different dimension pressure sensor and so forth All of these parts deform when pressurized The sum of this deformation is called pump elasticity The performance of the pump is greatly enhanced by correcting for this elasticity The Pump Elasticity Calibration calculates correction factors to compensate for the individual elasticity of the pump that was calibrated The elasticity is different for every pump and may change with the replacement of parts in the flow path e g pump seals All Binary Pumps SL are elasticity calibrated at the factory and require recalibration only after preventive maintenance or major repairs
24. Description 1 01018 22707 PTFE frit pack of 5 1 G1312 60061 Purge valve assembly 1 Using a 1 4 inch wrench disconnect the pump outlet capillary at the purge valve 2 Disconnect the waste tube Beware of leaking solvents due to hydrostatic pressure 3 Using the 14 mm wrench unscrew the purge valve and remove it from the purge valve holder 4 Remove the plastic cap with the gold seal from the purge valve 5 Use a pair of tweezers or a toothpick to remove the frit 154 1260 Binary Pump User Manual Maintenance 10 Valun Valve nging the Purge Valve Frit or the Purge 1 Valve body Purge valve assembly p n G1312 60061 2 PTFE frit pack of 5 p n 01018 22707 3 Seal cap p n 5067 4728 6 Place a new frit into the purge valve with the slit facing the seal cap 7 Replace the seal cap 8 Insert the purge valve into the purge valve holder and orient he waste outlet nozzle downward as shown below 9 Tighten the purge valve and reconnect outlet capillary and waste tubing Pump outlet capillary Purge valve Waste tube Figure 28 Exchanging the Purge Valve 1260 Binary Pump User Manual 155 10 Maintenance Removing the Pump Head Assembly When Tools required Preparations CAUTION Exchanging pump seals Exchanging pistons Exchanging seals of the seal wash option Wrench 1 4 inch 3 mm hexagonal key 4 mm hexagonal key 1 4 inch slitted socket wrench Switch off the pump at
25. Solvent compressibility No No Yes calibration Pump elasticity calibration No No Yes 1260 Binary Pump User Manual Troubleshooting and Diagnostics 7 Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues 1260 Binary Pump User Manual 127 7 Troubleshooting and Diagnostics Agilent Lab Advisor Software 128 1260 Binary Pump User Manual 1260 Binary Pump User Manual
26. The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 13 on page 41 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed CAUTION Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 38 1260 Binary Pump User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the
27. Ts tf aj cuff Waste tubing Outlet capillary to autosampler Waste outlet Figure 18 Flow Connection of Binary Pump without Solvent Selection Valve 1260 Binary Pump User Manual 63 3 Installing the Pump Priming the System Initial Priming When Parts required Preparations Before a new degasser or new solvent tubing can be used it is necessary to prime the system Isopropanol IPA is recommended as priming solvent due to its miscibility with nearly all HLPC solvents and its excellent wetting properties Description 1 lsopropanol Connect all modules hydraulically as described in the respective module manuals Fill each solvent bottle with 100 mL isopropanol Switch the system on When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can bear health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 64 The purge tool of the LabAdvisor or Instrument Utilities can be used for automatically purging the pump If the pump is not able to aspirate the solvent from the bottles a syringe can be used to draw the solvent manually through tubing and degasser 1260 Binary Pump User Manual Installing the Pump 3 When priming the vacuum
28. adapter part no 0100 1847 onto the Active Inlet Valve and connect the inlet tube directly to it 3 Connect the restriction capillary part no 5022 2159 to the purge valve Put its other end into a waste container 4 Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 mL min 5 Close the purge valve set the flow to a value that gives a pressure of 350 bar Pump 15 min at this pressure to wear the seals in The pressure can be monitored on the analog output connector of the pump with the Instant Pilot chromatographic data system or any other controlling device connected to your pump 6 Turn the pump Off slowly open the purge valve to release the pressure from the system disconnect the restriction capillary and reconnect the outlet capillary to the purge valve Reconnect the intake tubing to the solvent selection valve and the connecting tube from solvent selection valve if installed to the AIV 7 Purge your system with the solvent used for your next application 1260 Binary Pump User Manual 161 10 Maintenance Exchanging the Pistons When When scratched Tools required 3 mm hexagonal key 4 mm hexagonal key Parts required p n Description 1 5063 6586 Piston Preparations Switch off the pump at the main power switch Remove the front cover to have access to the pump mechanics Removing the Pump Head Assembly on page 156 Disassembling the pump head on page 1
29. installed correctly screws Install the pump head correctly Ensure nothing not secured or pump head not seated e g capillary is trapped between the pump correctly head and body 2 Broken piston Exchange the piston Index Limit Index Limit Pump channel A B Index Limit Pump channel B The time required by the piston to reach the encoder index position was too short pump During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is reached too fast the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative 1260 Binary Pump User Manual 117 118 Error Messages Index Adjustment Index Adjustment Pump channel A B Index Adjustment Pump channel B The encoder index position in the module is out of adjustment During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the time to reach the index position is too long the error message is generated Probable caus
30. next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces Some modules have module specific interfaces connectors They are described in the module documentation 1260 Binary Pump User Manual 29 1 Introduction to the Binary Pump Setting the 8 bit Configuration Switch Setting the 8 bit Configuration Switch On Board LAN The 8 bit configuration switch is located at the rear of the module Switch settings provide configuration parameters for LAN serial communication protocol and instrument specific initialization procedures All modules with on board LAN e g G1315 65C D G1314D E F G4212A B G4220A e Default is ALL switches DOWN best settings Bootp mode for LAN e For specific LAN modes switches 3 8 must be set as required e For boot test modes switches 1 2 must be UP plus required mode Figure 5 Location of Configuration Switch example shows a G4212A DAD To perform any LAN configuration SW1 and SW2 must be set to OFF For details on the LAN settings configuration refer to chapter LAN Configuration 30 1260 Binary Pump User Manual Introduct
31. of the peristaltic pump can be controlled from the data system or the Instant Pilot The binary pump comes with pre installed seal wash capable support rings When the user decides to use seal wash it is recommended to replace the secondary seals and gaskets by new ones to ensure tightness For information on the installation of the continuous seal wash option refer to the Service Manual 1260 Binary Pump User Manual 89 5 Optimizing Performance When to Use Alternative Seals The standard seals for the binary pump can be used for most applications However normal phase applications for example hexane are not compatible with the standard seals They cause extremely high abrasion and significantly shorten seal life time For the use with normal phase applications special polyethylene pistons seals yellow color PE seals pack of 2 p n 0905 1420 are available These seals have less abrasion compared to the standard seals WARNING The seal wear in procedure causes problems to the normal phase seals yellow They will be destroyed by the procedure gt DO NOT apply the seal wear in procedure performed to normal phase seals 1 Remove the standard seals from the pump head Exchanging the Pump Seals on page 159 2 Install normal phase seals Polyethylene seals have a limited pressure range of 0 200 bar When used above 200 bar their lifetime will be significantly reduced 90 1260 Binary Pump User Manual Op
32. power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment 1260 Binary Pump User Manual 39 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 13 on page 41 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric conne
33. product user guides CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 1260 Binary Pump User Manual 149 10 Maintenance Cleaning the Module The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth as liquid may drip into the module WARNING Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings 150 1260 Binary Pump User Manual Maintenance 10 Overview of Maintenance and Simple Repairs Figure 27 on page 151 shows the main user accessible assemblies of the binary pump The pump heads and its parts do require normal maintenance for example seal exchange and can be accessed from the front simple repairs Replacement of valve cartridges or filters don t require to remove the pump from the system stack Figure 27 Overview of Maintenance and Simple Repair Procedures 1 Purge valve Exchanging the Purge Valve Frit or the Purge Valve on
34. risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Cleaning the Solvent Filters Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 35 for one hour Thoroughly flush the filter with HPLC grade water remove all nitric acid some capillary columns can be damaged by nitric acid Replace the filter Never use the system without solvent filter installed 1260 Binary Pump User Manual 87 5 Optimizing Performance When to Use a Vacuum Degasser The binary pump does not necessarily require degassing However for the following conditions a vacuum degasser is mandatory e Your detector is used with maximum sensitivity in the low UV wavelength range e Your application requires highest injection precision or Your application requires highest retention time reproducibility flow rates below 0 5 mL min e The binary pump is used with bypassed damper and mixer Operational Hints for the Vacuum Degasser If you are using the vacuum degasser for the first time if the vacuum degasser was switched off for any length of time for example overnight or if the vacuum degasser chambers are empty you have to prime the vacuum degasser before running an a
35. sure that the pump is very thoroughly flushed with water before starting the test Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to generate misleading results 1 Select the pump test from the test selection menu 2 Start the test and follow the instructions Make sure to release the pressure by opening the purge valve when the test has finished completed Otherwise the pump may generate an overpressure error Evaluating the Results Refer to the help file of the Agilent Lab Advisor for further details 1260 Binary Pump User Manual 135 Tests Functions and Calibration Binary Pump Solvent Calibration 136 Description Each solvent or solvent mixture has unique compressibility at different pressures In order to deliver accurate flow with minimal pressure and composition ripple over the full operational pressure range it is necessary that the pump compensates precisely for the compressibility of the solvents in use The binary pump comes with compressibility parameters for the most common HPLC solvents and solvent mixtures If a solvent is not available in the list of pre calibrated solvents the solvent compressibility calibration allows the appropriate compressibility data to be generated Technical background The solvent compressibility calibration relies on an accurate elasticity calibration of the pump With a proper elasticity calibration in place the pump is
36. switched into pressure control mode A restriction capillary is connected to the purge valve outlet By varying the flow rate the pump maintains a certain pressure The pump optimizes the compressibility value of the solvent until the lowest possible pump ripple is reached The pump increases the flow rate and adjusts the pressure to the next calibration step where the pump ripple is minimized again This process is repeated until solvent compressibility data for the whole operating pressure range of the pump are available The compressibility data set for this solvent is stored in an XML file in C Documents and Settings lt username gt Application Data Agilent Technologies Agilent Lab Advisor 2 02 0 0 data It can be shared with other G1312B pumps via the controlling data system 1260 Binary Pump User Manual Tests Functions and Calibration 8 Running the Solvent Compressibility Calibration When Tools required Parts required Preparations CAUTION Running the Solvent Compressibility Calibration from the Agilent Lab Advisor If a solvent is not available in the list of pre calibrated solvents the solvent compressibility calibration allows to generate appropriate compressibility data Wrench 1 4 5 16 inch Description G1312 67500 Calibration capillary assembly Place a bottles with solvent to be calibrated in channel A resp A1 if a solvent selection valve is installed Avoid inaccurate pump elasticity calibration
37. the G4208A Instant Pilot Generic operation of the G4208A Instant Pilot is covered in the Instant Pilot User s Guide part number G4208 90000 Details about setting up module specific parameters can be found in the Instant Pilot online help The pump parameters are described in depth in Overview on page 73 72 1260 Binary Pump User Manual Using the Pump 4 Setting up the Pump with Agilent ChemStation Overview Most of these panels can be accessed in two different ways Pulling down the Instrument menu or left clicking on the icon the GUI Setup of Basic Pump Parameters The most important parameters of the pump are grouped in the Set up Pump panel Open it either from the Instrument menu or by left clicking the pump icon in the graphical user interface GUD p Control Pressure Limits Flow StopTime PostTime Nite 0 800 ml min 5 00 min 7 00 min o bar SNA Min Solvent type Solvent A 97 0 ho Ej om Solvent comment pee 30a h2 z J j Timetable i Time _ ZB __ Flow Max Press Insert 1 oo 30 2 4o 0 0 Lara Gat Copy Paste Display Timetable Timetable Flow Pr Caneel Heb sovens gt Figure 19 Set up Pump Panel 1260 Binary Pump User Manual 73 4 Using the Pump Table 16 Parameters of the Set up Pump Panel Parameter Limits Description Flow 0 001 5 mL min Total flow rate of the pump See When to Remove D
38. the main board Probable cause 1 Loose fittings 2 Broken capillary 3 Loose or leaking purge valve inlet valve or outlet valve 4 Defective pump seals Leak Sensor Open Suggested actions Ensure all fittings are tight Exchange defective capillaries Ensure pump components are seated correctly If there are still signs of a leak exchange the appropriate seal purge valve inlet valve outlet valve Exchange the pump seals The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause 1 Leak sensor not connected to the main board 2 Defective leak sensor 3 Leak sensor incorrectly routed being pinched by a metal component Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative 1260 Binary Pump User Manual Error Messages 6 Leak Sensor Short The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increas
39. the pump test check both pump heads individually Tools required Wrench 1 4 inch 3 mm hexagonal key 4 mm hexagonal key Insert tool Parts required p n Description 1 0905 1503 Piston seal PTFE carbon filled black pack of 2 default 1 0905 1420 PE seals pack of 2 1 5022 2159 Restriction capillary Preparations Switch off the pump at the main power switch Remove the front cover to have access to the pump mechanics 1 Disassemble the pump head assembly see Disassembling the pump head on page 158 2 Use the insert tool to carefully remove the seal from the pump head Se Insert tool A Pump seal Pump head 1260 Binary Pump User Manual 159 10 Maintenance Exchanging the Pump Seals 3 Using the insert tool insert the new seals into the pump head and press them firmly in position Insert tool Pump seals 4 Reassemble the pump head assembly see Reinstalling the Pump Head Assembly on page 165 NOTE Reset the seal wear counter and liquimeter in the Agilent Lab Advisor 160 1260 Binary Pump User Manual Maintenance 10 Seal Wear in Procedure This procedure is required for standard seals only part no 5063 6589 but it will definitely damage the normal phase application seals part no 0905 1420 1 Puta bottle with 100 ml of isopropanol in the solvent cabinet and place the solvent intake filter of the pump head you want to wear in into this bottle 2 Screw the PEEK
40. the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended 1260 Binary Pump User Manual 13 1 introduction to the Binary Pump Overview of the Hydraulic Path Overview of the Hydraulic Path The solvent from the bottle in the solvent cabinet enters the pump through an active inlet valve Each side of the binary pump comprises two substantially identical pump units Both pump units comprise aball screw drive and a pump head with two sapphire pistons for reciprocating movement gt To mixing chamber Inlet From solvent bottle valve rH Piston Ball screw drive Motor with encoder 14 1260 Binary Pump User Manual Introduction to the Binary Pump 1 A servo controlledvariable reluctance motor drives the two ball screw drives in opposite directions The gears for the ball screw drives have different circumferences ratio 2 1 allowing the first piston to move at double the speed of the second piston The solvent enters the pump heads close to the bottom limit and leaves it at its top The outer diameter of the piston is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap in between The first piston has a stroke volume in the range of 20 uL to 100 uL depending on the flow rate The microprocessor controls all flow rates in a range of 1 uL min to 5 mL min The inlet of the first pumping unit is connected to the active
41. to the flow path Replacement of capillaries or PTFE frits are not considered a major repair What is Solvent Compressibility Compensation Although the compressibility of liquids is orders of magnitude lower then the compressibility of gases a noticeable volume error is seen when typical chromatographic solvents are compressed to operating pressures as high as 600 bar In addition the compressibility changes with pressure temperature and the amount of dissolved gas In order to minimize the influence of the latter the use of a vacuum degasser is mandatory for high precision delivery of liquids Unfortunately the influence of the temperature on compressibility is non linear and cannot be calculated The Agilent Binary Pump SL features a new multi point compressibility calibration The compressibility of a solvent is determined at different pressures from 0 600 bar and stored in an XML file This file can be distributed to other pumps because the solvent compressibility is independent from the pump The Binary Pump SL and ChemStation come with predetermined solvent compressibility data for the most common HPLC solvents like water acetonitrile methanol etc Users can calibrate their own solvent mixtures with the help of an easy to use calibration procedure in the Agilent LC Diagnostic software 1260 Binary Pump User Manual Introduction to the Binary Pump 1 A correct pump elasticity calibration is an essential prerequisite for suc
42. with water 1260 Binary Pump User Manual Site Requirements and Specifications 2 Table 14 Performance Specifications of the Agilent 1260 Infinity Binary Pump G1312B Type Specification Comments Composition range settable range 0 100 recommended range 1 99 or 5 L min per channel whatever is greater Composition precision lt 0 15 RSD or lt 0 04 min SD whichever is at 1 mL min greater Composition accuracy 0 35 absolute water caffeine tracer Control Agilent ChemStation for LC 32 bit G4208A Revision B 02 00 or above Handheld Controller EZ Chrom Elite Analog output For pressure monitoring 1 33 mV bar one output Communications Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN optional For use with flow rates below 500 l min or for use without damper and mixer a vacuum degasser is required All specification measurements are done with degassed solvents 1260 Binary Pump User Manual 43 2 44 Site Requirements and Specifications Performance Specifications 1260 Binary Pump User Manual 1260 Binary Pump User Manual 3 Installing the Pump Unpacking the Binary Pump 46 Optimizing the Stack Configuration 48 Installing the Binary Pump 55 Flow Connections with Solvent Selection Valve 58 Flow Connections without Solvent Selection Valve 61 Priming the System 64 Initial Priming 64 Regular Priming 66 Changing Solvents 67 This chapte
43. 00 RRLC in Low Delay Volume Configuration with Post Column Cooler Degasser Pump HiPALS 0 12 mm seat Cooler for ALS TCC DAD SL This configuration is usually used for short 2 1 mm and 3 0 mm columns optimized for high flow rates For a more detailed help on configuring your instrument refer to the RRLC system configurator A 01 01 CD ROM p n 01200 60001 400 mm x 0 17 mm ca 320 mm x 0 12 mm New LowDelayVolmne Heat Exchanger New LDV post column cooler 0 12 mmID cap 2 ul cell Figure 11 1200 RRLC system in low delay volume configuration for 2 1 mm amp 3 0 mm id columns 1260 Binary Pump User Manual 53 el iv A ts ing the otacK CONTIG 3 Installing the Pump 1200 RRLC in Low Delay Volume Configuration with Automated Column Regeneration and MS This is the recommended setup to achieve minimum cycle time using MS detection For a more detailed help on configuring your instrument refer to the RRLC system configurator A 01 01 CD ROM p n 01200 60001 DAD or MWD 2 ul cell New LDV post colum cooler 0 12 mmID cap ca 320 mm x 0 12 mm Degasser Reg Pump WPS 0 12 mm seat Pump ee x w Cooler f WPS ToF MSD 650 mm x 0 17 mm Figure 12 1200 RR with Automated column regeneration and TOF in low delay volume configuration 54 1260 Binary Pump User Manual Installing the Pump 3 Installing the Binary Pump Parts required Preparations p n Descr
44. 3 10 Maintenance Using the insert tool press the seal spring pointing upwards into the recess of the support ring Place a seal wash gasket in the recess of the support ring and replace the seal keeper Insert tool Seal S upport rin g 4 Reassemble the pump head assembly see Reinstalling the Pump Head Assembly on page 165 164 1260 Binary Pump User Manual Maintenance 10 Reinstalling the Pump Head Assembly When When reassembling the pump Tools required 3 mm hexagonal key 4 mm hexagonal key Parts required p n Description 1 79846 65501 Pump head grease 1 Slide the pump head assembly onto the pump drive Purge valve holder Pump head screws l 2 Using a 4 mm hexagonal key tighten the pump head screws stepwise with increasing torque 3 Using a3 mm hexagonal key fix the purge valve holder to the pump head 1260 Binary Pump User Manual 165 10 Maintenance Reinstalling the Pump Head Assembly 4 Reconnect the tubing and capillaries to the connector 166 1260 Binary Pump User Manual Maintenance 10 Exchanging the Inlet Valve When If internally or externally leaking Tools required Wrench 14 mm Parts required Description G1312 60066 Passive inlet valve Preparations Switch off the pump at the main power switch 1 Remove the front cover 2 Disconnect the solvent inlet tube at the inlet valve beware of leaking solvents 3 Using a 14 mm wrench loosen
45. 3 control and data evaluation 43 performance 42 status indicator 125 stroke volume 13 stroke length 119 stroke volume 15 18 synchronization lost 103 T temperature limit exceeded 113 temperature out of range 113 temperature sensor 104 test functions 122 tests pressure test results 132 pump elasticity calibration 139 running the pressure test 131 solvent compressibility calibration 136 137 timeout 102 tools blank nut 1 16 inch 131 screwdriver pozidriv 1 156 screwdriver pozidriv 1 1 4 wrench 1 4 inch slitted socket 156 wrench 1 4 5 16 inch 131 wrench 14mm 169 wrench 1 4 5 16 inch 135 137 139 troubleshooting errormessages 101 122 status indicators 122 124 U unpacking the pump 46 V vacuum degasser 8 43 70 70 operational hints 88 when to use 88 valve inlet valve replacement 153 solvent selection 174 variable reluctance motor 15 variable stroke volume 13 variable stroke volume 18 voltage range 41 1260 Binary Pump User Manual Index Ww wash seals 163 wash seals 152 wash solvent 89 waste electrical and electronic equipment 225 WEEE directive 225 weight 41 wrench 1 4 5 16inch 131 135 137 139 wrench 1 4 inch slitted socket 156 wrench 1 4 inch 154 wrench 14mm 154 167 169 Z zero solvent counter 108 1260 Binary Pump User Manual 235 www agilent com In This Book This manual contains technical reference information about the Agilent 1260 Infinity Binary Pump G1
46. 312 68721 p n Description 5065 9953 Seal wash pump assembly 5042 8507 Seal wash pump cartridge silicone tubing 0905 1175 Secondary seal pre installed in support rings 5062 2484 Gasket seal wash pack of 6 0890 1764 Silicone rubber tubing 1 mm i d 3 m 5063 6589 2x Standard seals pack of 2 01018 2370 Seals insert tool 1260 Binary Pump User Manual 201 11 Parts and Materials for Maintenance G1316B SL Capillary System Kit 202 p n G1316 83200 G1316 80002 G1316 80003 G1316 80004 G1316 68716 Description Carrier for Heater and Cooler Heater long up 0 12 mm i d 1 6 uL internal volume Heater long down 0 12 mm i d 1 6 pL internal volume Post column cooler 0 12 mm i d 1 5 uL internal volume Capillary System Kit For items see also Heater and Cooler Devices for G1316B in the G1316B User Manual 1260 Binary Pump User Manual p n G1367 87303 G1315 87339 G1316 87319 G1316 87318 G1316 87317 G1316 87316 G1316 87315 G1316 87314 61316 87313 G1316 87312 G1316 87327 61316 87309 G1315 87307 1260 Binary Pump User Manual Parts and Materials for Maintenance 11 Description Seat capillary 150 mm 0 12 mm i d DAD Heat Exchanger Capillary 310 mm 0 12 mm i d SST Capillary 340 mm 0 12 mm i d m m SST Capillary 300 mm 0 12 mm i d m m SST Capillary 210 mm 0 12 mm i d m m SST Capillary 170 mm 0 12 mm i d m m SST Capillary 130 mm 0 12 mm i d m f SST Capilla
47. 312B The manual describes the following introduction e site requirements and specifications e installing the pump using the binary pump e optimizing performance e troubleshooting and diagnostics maintenance e parts and materials for maintenance e identifying cables appendix Agilent Technologies 2005 2008 2010 Printed in Germany 06 10 G1312 90012 fe Agilent Technologies
48. 58 1 Check the plunger surface and remove any deposits or layers Most suitable is polishing of the plunger rod with toothpaste Replace the plunger if scratched of if dents are visible A Piston surface Figure 29 Plunger The best way to inspect a piston is to hold it up and watch e g a light bulb though the piston rod The transparent sapphire acts as a very strong magnifier and even smallest surface abnormalities become visible 162 1260 Binary Pump User Manual Exchanging the Wash Seals Tools required hexagonal key3 mm 4 mm hexagonal key Insert tool Small flat head screwdriver Maintenance 10 Parts required p n Description 1 0905 1175 Wash seal 1 5062 2484 Gasket seal wash pack of 6 Preparations Switch off the pump at the main power switch Remove the front cover to have access to the pump mechanics Removing the Pump Head Assembly on page 156 Disassembling the pump head on page 158 1 Remove the seal keeper and the seal wash support rings from the plunger housing Remove the seal keeper from the support ring assembly Seal keeper s 7 SS Ss X Ss Seal wash support ring gt gt 7 gt y Plunger housing Plunger 2 Using the blade of a flat head screwdriver remove seal wash gasket and the secondary seal from the support ring Nd Secondary seal T L f gt D Seal wash support ring m 1260 Binary Pump User Manual 16
49. A B Servo Restart Failed Pump channel B The pump motor in the module was unable to move into the correct position for restarting When the module is switched on the first step is to switch on the C phase of the variable reluctance motor The rotor should move to one of the C positions The C position is required for the servo to be able to take control of the phase sequencing with the commutator If the rotor is unable to move or if the C position cannot be reached the error message is generated Probable cause Suggested actions 1 Disconnected or defective cable Please contact your Agilent service representative 2 Mechanical blockage of the module Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly 3 Defective pump drive assembly Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative 116 1260 Binary Pump User Manual Error Messages 6 Pump Head Missing Pump Head Missing Pump channel A B Pump Head Missing Pump channel B The pump head end stop in the pump was not found When the pump restarts the metering drive moves forward to the mechanical end stop Normally the end stop is reached within 20 seconds indicated by an increase in motor current If the end point is not found within 20 seconds the error message is generated Probable cause Suggested actions 1 Pump head not
50. Fan Failed The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 4 Improperly positioned cables or wires Ensure the fan is not mechanically blocked obstructing fan blades 1260 Binary Pump User Manual Error Messages 6 Open Cover The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during operation Reinstall the top foam 2 Foam not activating the sensor Please contact your Agilent service representative 3 Dirty or defective sensor Please contact your Agilent service representative 4 Rear of the module is exposed to strong Ensure that the rear of module is not directly direct sunlight exposed to strong sunlight 1260 Binary Pump User Manual 107 6 Error Messages Module Error Messages Restart Without Cover The modul
51. ad assembly onto the metering drive Apply a small amount of pump head grease part no 79846 65501 to the pump head screws and the balls of the spindle drive Tighten the pump head screws stepwise with increasing torque until they are tight 15 Reconnect all capillaries tubes and the active inlet valve cable to its connector 16 Route the wash tubings as shown in inlet tube into a bottle filled with a mixture of distilled water and isopropanol 90 10 and place the bottle in the solvent cabinet 1260 Binary Pump User Manual Maintenance 10 17 Route the outlet of the wash tube back into the wash solvent bottle Figure 34 Routing of Active Seal Wash Tubing 1260 Binary Pump User Manual 181 10 Maintenance Exchanging the Optional Interface Board When Board defective Parts required Description 1 BCD Interface board CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD can damage electronic boards and components gt In order to prevent damage always use an ESD protection when handling electronic boards and components 1 Switch OFF the module at the main power switch Unplug the module from main power Disconnect cables from the interface board connectors Loosen the screws Slide out the interface board from the module Install the new interface board Secure the screws oa A Ww N Reconnect the cables to the board connector BCD Interface board
52. al 1260 Binary Pump User Manual 000 J Ja Using the Pump Hints for Successful Use of the Binary Pump 70 Setting up the Pump with the G4208A Instant Pilot 72 Setting up the Pump with Agilent ChemStation 73 Overview 73 Setup of Basic Pump Parameters 73 Pump Control 75 Pump Auxiliary Parameters 77 DataCurves 79 Bottle Filling 80 Early Maintenance Feedback EMF 82 EMF Counters 82 Using the EMF Counters 83 This chapter explains the operational parameters of the binary pump ee Agilent Technologies 4 Using the Pump Hints for Successful Use of the Binary Pump 70 Place solvent cabinet with the solvent bottles always on top or at a higher level of the pump When using the binary pump without vacuum degasser shortly degas your solvents for example water vacuum pump for 15 30 s in an appropriate vessel before using them in the pump If possible apply solvent conditions that will decrease the gas solubility over time for example warming up the solvents The use of avacuum degasser is mandatory for flow rates below 0 5 mL min and for configurations without damper and mixer When using the binary pump with vacuum degasser flush the degasser with at least 5 mL per channel before operating the pump especially when the pumping system had been turned off for a certain length of time for example overnight and volatile solvent mixtures are used in the channels see Regular Priming on page 66
53. amper and Mixer on page 92 for pump hardware modifications to achieve lowest delay volume Stop Time 0 01 min no limit The stop time of the pump usually controls the run time of the whole LC system Use no limit to stop the run manually useful for method development PostTime off 99999 min Time between the end of a run and the start of the next Used for column equilibration after a gradient Pressure Max 0 600 bar Max must be bigger than Min Set max pressure to the maximum operating Limits Min 0 600 bar pressure of your column A min pressure setting of e g 10 bar will turn off your pump automatically when running out of solvent A smarter way however is to use the bottle fillings function see Bottle Filling on page 80 SolventA 0 100 Although channel A can be set to 0 it cannot be turned off This channel should be used for the aqueous phase water SolventB off 100 The percentage of channel B is automatically complemented by channel A to give 100 Solvent H20 ACN MeOH IPA Select the solvent you are using in the respective solvent channel from the type drop down list In case your solvent is not listed perform a solvent compressibility calibration see Running the Solvent Compressibility Calibration on page 137 For details on solvent compressibility see Binary Pump Solvent Calibration on page 136 Solvent Free text field for a description of the solvent This description will show up in
54. anual 133 8 Tests Functions and Calibration Pump Test 134 Description The pump test provides a fast and accurate way to verify proper hydraulic operation of the binary pump Problems related to defective valves seals or pistons can be diagnosed and usually the defective part is identified Step 1 The system is setup with water on both channels and a restriction capillary is attached to the outlet of the pump Pump head A is delivering at 1 mL min The pressure signal is monitored and overlaid with the piston movement plot The pressure pattern and the slope of the pressure signal are evaluated for the delivery strokes of both pistons Step 2 The procedure from step 1 is repeated on pump head B Step 3 The data from step 1 and 2 are evaluated In case test failed a conclusion about the defective part is made 1260 Binary Pump User Manual Tests Functions and Calibration 8 Running the Pump Test When Tools required Parts required Preparations Running the test from the Agilent Lab Advisor The test should be used to prove proper operation of the binary pump after repairs or when a the pressure test see Pressure Test Description on page 130 determined a problem with the pump Wrench 1 4 5 16 inch Description G1312 67500 Calibration capillary assembly Place two bottles of HPLC grade water in channels A and B A1 and B1 if the pump is equipped with a solvent selection valve Make absolutely
55. anufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation 222 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation 1260 Binary Pump User Manual Appendix 13 Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders m
56. ash ol 0905 1175 Wash seal 01018 07102 Gasket Seal wash 7 G4220 26210 Seal Holder 8 G4220 24013 Backup Ring for Seal Holder 9 G1312 87300 Absorber capillary 10 0905 1503 Piston seal PTFE carbon filled black pack of 2 default 11 0515 0175 Mounting screw for manual purge valve holder M4 20 mm long 12 G1312 23200 Holder for manual purge valve 13 G1312 60061 Purge valve assembly 14 G1312 60067 Outlet valve complete 15 5042 1303 Screw lock 16 G1312 25260 Pump housing 17 G1312 60066 Passive inlet valve 18 G1312 23201 Adapter 19 0515 2118 Screw M5 60 mm long 194 1260 Binary Pump User Manual Parts and Materials for Maintenance Pump Head Assembly with Seal Wash Option 11 Figure 40 1260 Binary Pump User Manual Pump Head Assembly With Seal Wash Option 195 11 Parts and Materials for Maintenance Outlet Valve Outlet Valve p n Description G1312 60067 Outlet valve complete Figure 41 Outlet Valve 196 1260 Binary Pump User Manual Purge Valve Assembly Item p n 1 G1312 60061 2 01018 22707 3 5067 4728 1260 Binary Pump User Manual Parts and Materials for Maintenance 11 Purge Valve Assembly Description Purge valve assembly PTFE frit pack of 5 Seal cap 197 11 Parts and Materials for Maintenance Active Inlet Valve Assembly Active Inlet Valve Assembly Item p n Description 1 G1312 60025 Active inlet valve body 2 G1312 60020 Cartridge for active inlet valve 600bar
57. ate is constantly monitored and adjusted The pump continues to pump until a system pressure of around 600 bar is reached Step 2 When the system pressure reaches 600 bar the pump continues to pump at a flow rate that keeps the pressure constant The flow that is needed to keep the pressure constant is directly translated into a leak rate 1260 Binary Pump User Manual Tests Functions and Calibration 8 Positioning the Blank Nut If a specific component is suspected of causing a system leak place the blank nut immediately before the suspected component then run the Pressure Test again If the test passes the defective component is located after the blank nut Confirm the diagnosis by placing the blank nut immediately after the suspected component The diagnosis is confirmed if the test fails Running the Pressure Test When Tools required Preparations Running the test from the Agilent Lab Advisor application The test should be used when problems with small leaks are suspected or after maintenance of flow path components e g pump seals injection seal to prove pressure tightness up to 600 bar e Wrench 1 4 5 16 inch 1 16 inch blank nut Place two bottles of HPLC grade water in channels A and B A1 and B1 if the pump is equipped with a solvent selection valve Make absolutely sure that all parts of the flow path that are part of the test are very thoroughly flushed with water before starting to pressurize the syste
58. bubble inside the flow path definitely will cause the calibration to fail If a solvent selection valve is installed flush all four solvent channels to avoid that air from a dry solvent intake tube is drawn into the flow path upon initialization 1 Select the pump elasticity calibration from the test selection menu 2 Start the test and follow the instructions Make sure to release the pressure by opening the purge valve when the test has finished completed Otherwise the pump may generate an overpressure error 1260 Binary Pump User Manual 139 8 Tests Functions and Calibration Pump Elasticity Calibration 140 1260 Binary Pump User Manual 1260 Binary Pump User Manual 9 Diagnostic Signals Analog Pressure Output 142 Description 142 Diagnostic Signals in the ChemStation Software 143 Directly Accessible Signals 143 Hidden Signals 143 Early Maintenance Feedback EMF 144 EMF Counters 144 Using the EMF Counters 145 This chapter explains all diagnostic signals and counter of the Binary Pump SL te Agilent Technologies 141 9 Diagnostic Sign als Analog Pressure Output Description Analog pressure output use analog signal cable 35900 60750 with Agilent Integrators of A D converters A BNC connector at the rear of the Binary Pump SL provides the reading of the pressure sensor as analog value with a resolution of 1 33 mV bar The maximum reading of 660 bar equals 800 mV The signal is available in real time and can be
59. c acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCly O gt gt 2COCl 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF 1260 Binary Pump User Manual 229 13 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download 230 1260 Binary Pump User Manual Index Index 8 bit configuration switch on board LAN 30 without On Board LAN 32 A absorber capillary 15 active inlet valve 198 Active seal wash seal wash active 8 active seal wash 89 adapter 62 Agilent Diagnostic software 127 Agilent Lab Advisor software 127 Agilent Lab Advisor 127 131 Agilent oninternet 230 algae growt
60. ce the pressure pulsation to below 2 of system pressure which is sufficient for most applications If the compressibility values for the solvents used differ from the default settings it is recommended to change the compressibility values accordingly Compressibility settings can be optimized by using the values for various solvents described in Table 19 on page 97 If the solvent in use is not listed in the compressibility table when using premixed solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the compressibility settings 1 Start channel A of the binary pump with the required flow rate 2 Before starting the optimization procedure the flow must be stable Use degassed solvent only Check the tightness of the system with the pressure test see Pressure Test Description on page 130 3 Your pump must be connected to an Agilent data system or Instant Pilot the pressure and ripple can be monitored with one of these instruments otherwise connect a signal cable between the pressure output of the isocratic pump and a recording device for example 339X integrator and set following parameters Zero 50 Att 2 3 Chart Speed 10 cm min 4 Start the recording device in plot mode 5 Starting with a compressibility setting of 10 x 10 bar increase the value in steps of 10 Re zero the integrator as required The compressibility compensation setting that ge
61. cessful solvent compressibility calibrations Solvent compressibility calibrations acquired with a miscalibrated pump will work but they are not transferable to other pumps How Does Variable Stroke Volume Work The smaller the solvent volume in the pump chamber is the faster it can be recompressed to operating pressure The Binary Pump SL allows to manually or automatically adjust the pump stroke volume of the first piston in the range of 20 100 uL Due to the compression of the solvent volume in the first pump chamber each piston stroke of the pump will generate a small pressure pulsation influencing the flow ripple of the pump The amplitude of the pressure pulsation is mainly dependent on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes generate less pressure pulsation than larger stroke volumes at the same flow rate In addition the frequency of the pressure pulsation will be higher This will decrease the influence of flow pulsations on quantitative results In gradient mode smaller stroke volume results in less flow ripple and improves the composition ripple The Binary Pump SL uses a processor controlled ball screw system to drive its pistons The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume The stroke volume for the pump is by default set to AUTO mode This means that
62. connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3394 Pin Agilent Signal Name Active module TTL Z 9 1 White Digital ground a NC 2 Brown Prepare run Low 3 3 Gray Start Low z NC 4 Blue Shut down Low 5 NC 5 Pink Not connected i NC 6 Yellow Power on High o 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators p n 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1260 Binary Pump User Manual Identifying Cables 12 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground gt NC 2 Brown Prepare run Low e 3 3 Gray Start Low m s NC 4 Blue Shut down Low e O a NC 5 Pink Not connected NC 6 Yellow Power on High o 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 A D Pin Agilent Signal Name Active module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low Zz 4 Blue 4 Blue Shut down Low 5 Pink 5 Pin
63. ctions If the bench should carry an Agilent system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 40 1260 Binary Pump User Manual Site Requirements and Specifications 2 Physical Specifications Table 13 Physical Specifications Type Specification Comments Weight 15 5 kg 34 Ibs Dimensions height x 180 x 345 x 435 mm 7 x 13 5 x 17 inches width x depth Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 220 VA 74 W 253 BTU Maximum Ambient operating 0 55 C 32 131 F temperature Ambient non operating 40 70 C 4 158 F temperature Humidity lt 95 at 25 40 C 77 104 F Non condensing Operating Altitude Up to 2000 m 6562 ft Non operating altitude Up to 4600 m 15091 ft For storing the module Safety standards IEC CSA Installation Category II Pollution Degree 2 For indoor use only UL 1260 Binary Pump User Manual 41 2 Site Requirements and Specifications Performa
64. cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 1260 Binary Pump User Manual 21 1 Introduction to the Binary Pump Electrical Connections e The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems The interface board slot is used for external contacts and BCD bottle number output or LAN connections The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch e The power input socket accepts a line voltage of 100 240 VAC 10 witha line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the pow
65. damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1260 Binary Pump User Manual Contents Contents Introduction to the Binary Pump 7 Instrument and Operation 8 Overview of the Hydraulic Path 14 Early Maintenance Feedback 20 Instrument Layout 21 Electrical Connections 22 Interfaces 24 Setting the 8 bit Configuration Switch 30 Site Requirements and Specifications 37 Site Requirements 38 Physical Specifications 41 Performance Specifications 42 Installing the Pump 45 Unpacking the Binary Pump 46 Optimizing the Stack Configuration 48 Installing the Binary Pump 55 Flow Connections with Solvent Selection Valve 58 Flow Connections without Solvent Selection Valve 61 Priming the System 64 Using the Pump 69 Hints for Successful Use of the Binary Pump 70 Setting up the Pump with the G4208A Instant Pilot 72 Setting up the Pump with Agilent ChemStation 73 Early Maintenance Feedback EMF 82 1260 Binary Pump User Manual Contents Optimizing Performance 85 Prevent Blocking of Solvent Filters 86 When to Use a Vac
66. dapter pack of 4 Solvent inlet filter 20 pm 1260 Binary Pump User Manual I ce Figure 36 1260 Binary Pump User Manual Bottle Head Assembly Parts Parts and Materials for Maintenance Bottle Head Assembly 11 187 11 Parts and Materials for Maintenance Hydraulic Path with Solvent Selection Valve Item p n Description 1 G1322 67300 Kit of 4 solvent tubes for connection degasser to SSV including labels 2 G1312 60000 Solvent selection valve PN gives half of a complete solvent selection block 5041 8365 Blank plug for unused SSV channels 3 G1311 67304 Connecting tube SSV to AIV G1312 60066 Passive inlet valve G1312 60025 Active inlet valve body optional without cartridge G1312 60065 Pump Head with Seal Wash 4 5 6 G1312 60067 Outlet valve complete 7 G1312 87300 Absorber capillary 8 G1312 67302 Mixing capillary 9 G1312 87301 Restriction capillary mixing capillary to pressure sensor 10 5067 1527 Pressure sensor 11 G1312 87305 Capillary SSL 0 17 x 150 mm pressure sensor to damper 12 G1312 60031 Damper 13 G1312 87330 Mixer 14 G1312 87306 Capillary SSL 0 17 x 105 mm connections to solvent mixer G1312 04100 Bracket for solvent mixer 15 G1312 60061 Purge valve assembly 5042 8507 Seal wash pump cartridge silicone tubing 5065 9978 Tubing 1 mm i d 3 mm o d silicone 5 m for seal wash option 16 G1312 87303 SS Capillary 400 x 0 17 mm m m ps ps G1312 87304 SST capillary 700 mm 0 17 mm i d 1 32 1
67. ded using update utilities Table 11 Boot Resident Settings without on board LAN Mode Select SW1 sw2 SW3 SW4 SW5 SW6 SW7 Swe LAN TEST BOOT 1 1 1 0 0 0 0 0 No LAN TEST BOOT 1 1 0 0 1 0 0 0 1260 Binary Pump User Manual 35 1 introduction to the Binary Pump Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 12 Forced Cold Start Settings without on board LAN Mode Select SW1 sw2 SW3 sw4 SW5 SW6 SW7 Sws LAN TEST BOOT 1 1 0 0 0 0 0 1 No LAN TEST BOOT 1 1 0 0 1 0 0 1 36 1260 Binary Pump User Manual 1260 Binary Pump User Manual 6 ee 2 e Site Requirements and Specifications 8 Site Requirements 38 Physical Specifications 41 Performance Specifications 42 This chapter provides information about site requirements and specifications for the binary pump ot Agilent Technologies 37 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations
68. degasser with a syringe the solvent is drawn through the degasser tubes very quickly The solvent at the degasser outlet will therefore not be fully degassed Pump for approximately 10 minutes at your desired flow rate before starting an analysis This will allow the vacuum degasser to properly degas the solvent in the degasser tubes Open the purge valve of the pump Set the flow rate to 5 mL min Select channel Al Turn the flow on ao A WH Observe if the solvent in the tubing of channel A1 is advancing towards the pump If it isn t disconnect the solvent tubing from the solvent selection valve attach a syringe with a syringe adapter and pull the liquid through the degasser Reattach the tubing to the solvent selection valve 6 Pump 30 mL isopropanol to remove residual air bubbles 7 Switch to the next solvent channel and repeat steps 5 and 6 until all channels have been purged 8 Turn the flow off and close the purge valve 1260 Binary Pump User Manual 65 3 Installing the Pump Regular Priming When When the pumping system has been turned off for a certain time for example overnight air will rediffuse into the solvent channel between the vacuum degasser and the pump Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time Preparations Switch the system on The purge tool of the LabAdvisor or Instrument Utilities can be used for automaticall
69. e compressibility of gases a noticeable volume error is seen when typical chromatographic solvents are compressed to operating pressures as high as 600 bar In addition the compressibility changes with pressure temperature and the amount of dissolved gas In order to minimize the influence of the latter the use of a vacuum degasser is mandatory for high precision delivery of liquids Unfortunately the influence of the temperature on compressibility is non linear and cannot be calculated The Agilent 1260 Infinity Binary Pump features a new multi point compressibility calibration The compressibility of a solvent is determined at different pressures from 0 600 bar and stored in an XML file This file can be distributed to other pumps because the solvent compressibility is independent from the pump The binary pump and ChemStation come with predetermined solvent compressibility data for the most common HPLC solvents like water acetonitrile methanol etc Users can calibrate their own solvent mixtures with the help of an easy to use calibration procedure in the Agilent Lab Advisor software Let us use the practical example from the last section once again to understand how compressibility compensation works Piston 1 draws solvent at ambient pressure The movement direction is reversed and piston 1 now compresses the solvent until the operating pressure of the HPLC system is reached The outlet ball valve opens and solvent is pumped by
70. e Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative Index Missing Index Missing Pump channel A B Index Missing Pump channel B The encoder index position in the module was not found during initialization During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is not recognized within a defined time the error message is generated Probable cause Suggested actions 1 Disconnected or defective encoder cable Please contact your Agilent service representative 2 Defective pump drive assembly Please contact your Agilent service representative 1260 Binary Pump User Manual Error Messages 6 Stroke Length Stroke Length Pump channel A B Stroke Length Pump channel B The distance between the lower piston position and the upper mechanical stop is out of limits pump During initialization the module monitors the drive current If the piston reaches the upper mechanical stop position before expected the motor current increases as the module attempts to drive the piston beyond the mechanical stop This current increase causes the erro
71. e described in Overview of Maintenance and Simple Repairs on page 151 148 1260 Binary Pump User Manual Maintenance 10 Warnings and Cautions Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The amount of substances should be reduced to the minimal volume required for the analysis gt Do not operate the instrument in an explosive atmosphere Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the metal top cover of the module No serviceable parts inside gt Only certified persons are authorized to carry out repairs inside the module Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent
72. e was restarted with the top cover and foam open The sensor on the main board detects when the top foam is in place If the module is restarted with the foam removed the module switches off within 30 s and the error message is generated Probable cause Suggested actions 1 The module started with the top cover and Please contact your Agilent service foam removed representative 2 Rear of the module is exposed to strong Ensure that the rear of module is not directly direct sunlight exposed to strong sunlight Zero Solvent Counter The error message is triggered if the remaining volume in a solvent bottle falls below the set limit Probable cause Suggested actions 1 Volume in bottle below specified volume Refill bottles and reset solvent counters 2 Incorrect setting Make sure the set solvent volume matches the actual bottle filling and set the shutoff limit to a reasonable value e g 100 mL for 1 L bottles 108 1260 Binary Pump User Manual Error Messages 6 Pressure Above Upper Limit The system pressure has exceeded the upper pressure limit Probable cause Suggested actions 1 Upper pressure limit set too low Ensure the upper pressure limit is set to a value suitable for the analysis 2 Blockage in the flowpath after the damper Check for blockage in the flowpath The following components are particularly subject to blockage inline filter frit needle autosampler seat capillary autosampler sample loop aut
73. eals Only necessary when Seal Wash Option is installed Leaks at lower pump head side loss of wash solvent 152 1260 Binary Pump User Manual Table 24 Simple Repair Procedures Maintenance 10 Procedure Typical Frequency Notes Exchanging the Purge Valve Frit or the If internally leaking Purge Valve on page 154 Exchanging the Inlet Valve on page 167 If leaking externally If solenoid is defective Exchanging the Outlet Valve on page 169 If internally leaking Exchanging the Solvent Selection Valve on If internally leaking If page 174 solenoid is defective Installing the Active Seal Wash Option see When upgrading to Service Manual Active Seal Wash Solvent dripping out of waste outlet when valve is closed Error messages Inlet Valve Fuse or Inlet Valve Missing Pressure ripple unstable run leak test for verification Cross port flow Error message Valve Failed Recommended if buffers of gt 0 1M are used regularly 1260 Binary Pump User Manual 153 10 Maintenance Exchanging the Purge Valve Frit or the Purge Valve When Frit when plunger seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across the frit at a flow rate of 5 mL min of H20 with purge valve opened Purge valve if purge valve cannot be closed leak tight Tools required Wrench 1 4 inch Wrench 14mm Pair of tweezers or toothpick Parts required p n
74. ed the EMF flag in the user interface is displayed Using the EMF Counters The use of the EMF counter is described in Early Maintenance Feedback EMF on page 82 1260 Binary Pump User Manual 145 9 Diagnostic Signals Early Maintenance Feedback EMF 146 1260 Binary Pump User Manual 1260 Binary Pump User Manual 10 Maintenance Introduction to Maintenance and Repair 148 Warnings and Cautions 149 Cleaning the Module 150 Overview of Maintenance and Simple Repairs 151 Maintenance Procedures 152 Exchanging the Purge Valve Frit or the Purge Valve 154 Removing the Pump Head Assembly 156 Disassembling the pump head 158 Exchanging the Pump Seals 159 Seal Wear in Procedure 161 Exchanging the Pistons 162 Exchanging the Wash Seals 163 Reinstalling the Pump Head Assembly 165 Exchanging the Inlet Valve 167 Exchanging the Outlet Valve 169 Installation of the Solvent Selection Valve Upgrade Kit 171 Exchanging the Solvent Selection Valve 174 Installing the Active Seal Wash Option 177 Exchanging the Optional Interface Board 182 Replacing Module Firmware 183 This chapter describes the maintenance of the module ot Agilent Technologies 147 10 Maintenance Introduction to Maintenance and Repair The pump is designed for easy repair The most frequent repairs such as piston seal replacement and purge valve frit exchange can be done from the front side without removing the pump from the system stack These repairs ar
75. ed waste tube to the waste outlet of the pump leak handling system 9 Connect the pump outlet capillary pump to injection device to the outlet of the purge valve 1260 Binary Pump User Manual 59 3 Installing the Pump Flow Connections with Solvent Selection Valve 10 Purge your system prior to the first use see Initial Priming on page 64 Bottle head assembly Solvent cabinet Tube clip not visible Degasser Channel Al Pressure sensor 3 D TEM a Static Mixer Je Se F H Pr Purge valve eg ei Oa e Qa Pump head B Pump head A e DA Active inlet valve A l Te J Active inlet valve B Waste tubing Outlet capillary to autosampler Channel A2 Leak drain Solvent selection valve Channel B2 Figure 16 Binary Pump with Solvent Selection Valve 60 1260 Binary Pump User Manual Installing the Pump 3 Flow Connections without Solvent Selection Valve Parts required p n Description 1 Other modules 1 G1312 68755 Accessory Kit 1 G1312 68765 Accessory Kit 2 wrenches 1 4 5 16 inch for capillary connections Preparations Pump is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can bear health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the s
76. entative 6 Restriction capillary blocked at pre mixing Exchange restriction capillary union 114 1260 Binary Pump User Manual Error Messages 6 Encoder Missing Encoder Missing Pump channel A B Encoder Missing Pump channel B The optical encoder on the pump motor in the module is missing or defective The processor checks the presence of the pump encoder connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable cause Suggested actions 1 Defective or disconnected pump encoder Please contact your Agilent service connector representative 2 Defective pump drive assembly Please contact your Agilent service representative Inlet Valve Missing Inlet Valve Missing Pump channel A B Inlet Valve Missing Pump channel B The active inlet valve in the module is missing or defective The processor checks the presence of the active inlet valve connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable cause Suggested actions 1 Disconnected or defective cable Please contact your Agilent service representative 2 Disconnected or defective connection cable Please contact your Agilent service front panel to main board representative 3 Defective active inlet valve Exchange the active inlet valve 1260 Binary Pump User Manual 115 6 Error Messages Servo Restart Failed Servo Restart Failed Pump channel
77. er supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 22 1260 Binary Pump User Manual Introduction to the Binary Pump 1 Electrical Connections Serial Number Information ALL The serial number information on the instrument labels provide the following information CCXZZ00000 Format CC Country of manufacturing DE Germany X Alphabetic character A Z used by manufacturing ZZ Alpha numeric code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same module 00000 Serial number Rear view of the module Configuration switch Slot for interface board RS232 Remote Analog output CAN Bus NANAANNANANN Power Plug Security Lever Figure 3 Electrical Connections to the Binary Pump 1260 Binary Pump User Manual 23 1 introduction to the Binary Pump Interfaces Table 2 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD optional LAN on board RS 232 Analog APG Remote The Agilent 1200 Infinity Series modules provide the following interfaces Special Pumps G1310B Iso Pump G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G4220A B Bin Pump G1361A Prep Pump Samplers G1329B ALS G2260A Prep ALS G1364B FC PS G1364C FC
78. es above the upper limit the error message is generated Probable cause Suggested actions 1 Defective flow sensor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative Compensation Sensor Open The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 1260 Binary Pump User Manual 105 106 Error Messages Compensation Sensor Short The ambient compensation sensor NTC on the main board in the module has failed short circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative
79. etime of the solvent filter and will maintain the performance of the module e Use a sterile if possible amber solvent bottle to slow down algae growth e Filter solvents through filters or membranes that remove algae e Exchange solvents every two days or refilter e Ifthe application permits add 0 0001 0 001M sodium azide to the solvent e Place a layer of argon on top of your solvent Avoid exposure of the solvent bottle to direct sunlight Never use the system without solvent filter installed Checking the Solvent Filters The solvent filters are located on the low pressure side of the binary pump A blocked filter therefore does not necessarily affect the high pressure readings of the pump The pressure readings cannot be used to check whether the filters are blocked or not If the solvent cabinet is placed on top of the binary pump the filter condition can be checked in the following way Remove the solvent inlet tube from the inlet port of the solvent selection valve or the adapter at the active inlet valve If the filter is in good condition the solvent will freely drip out of the solvent tube due to hydrostatic pressure If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube 1260 Binary Pump User Manual Optimizing Performance 5 WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can bear health
80. ew 208 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n Description 35900 60750 Agilent module to 3394 6 integrators 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs Remote cables p n Description 03394 60600 Agilent module to 3396A Series integrators 3396 Series Il 3395A integrator see details in section Remote Cables on page 212 03396 61010 Agilent module to 3396 Series III 3395B integrators 5061 3378 Agilent module to Agilent 35900 A D converters or HP 1050 1046A 1049A 01046 60201 Agilent module to general purpose BCD cables p n Description 03396 60560 Agilent module to 3396 integrators G1351 81600 Agilent module to general purpose 1260 Binary Pump User Manual Identifying Cables 12 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable Agilent module interface board to general purposes RS 232 cables p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable
81. fed into an appropriate recording device e g integrator or strip chart recorder for troubleshooting purposes Figure 26 Location of Analog Output Connector 142 1260 Binary Pump User Manual Diagnostic Signals 9 Diagnostic Signals in the ChemStation Software Directly Accessible Signals In ChemStation the following instrument parameters are accessible during data acquisition and can be stored in the data file e actual pump pressure solvent composition gradient Hidden Signals Piston Movement When overlaid with the pump pressure signal this function allows to diagnose valve problems However it is recommended to use the pump test see Pump Test on page 134 instead as it is optimized for use with the Binary Pump SL The piston movement signal needs to be turned on by typing the following command into the ChemStation command line Ipmpdiagmode 1 ChemStation resets this function upon bootup It is necessary to turn it back on every time ChemStation is restarted If needed the function can be manually disabled by typing the command below into the ChemStation command line lIpmpdiagmode 0 1260 Binary Pump User Manual 143 9 Diagnostic Signals Early Maintenance Feedback EMF Components in the flow path are subject to mechanical wear or stress and require regular maintenance Ideally the frequency at which components are exchanged should be based on the intensity of usage of the inst
82. g the Solvent Selection Valve 174 Installing the Active Seal Wash Option 177 Exchanging the Optional Interface Board 182 Replacing Module Firmware 183 1 Parts and Materials for Maintenance 185 Bottle Head Assembly 186 Hydraulic Path with Solvent Selection Valve 188 Hydraulic Path without Solvent Selection Valve 190 Pump Head Assembly Without Seal Wash 192 Pump Head Assembly with Seal Wash Option 194 Outlet Valve 196 Purge Valve Assembly 197 Active Inlet Valve Assembly 198 Accessory Kit 199 Active Seal Wash Option 201 G1316B SL Capillary System Kit 202 Solvent Cabinet 204 Preventive Maintenance Kit G1312 68750 206 1260 Binary Pump User Manual Contents 12 Identifying Cables 207 Cable Overview 208 Analog Cables 210 Remote Cables 212 BCD Cables 215 CAN LAN Cables 217 External Contact Cable 218 RS 232 Cables 219 Agilent 1200 Module to Printer 220 13 Appendix 221 General Safety Information 222 The Waste Electrical and Electronic Equipment Directive 225 Lithium Batteries Information 226 Radio Interference 227 Sound Emission 228 Solvent Information 229 Agilent Technologies on Internet 230 6 1260 Binary Pump User Manual 1260 Binary Pump User Manual 1 Introduction to the Binary Pump Instrument and Operation 8 Instrument Layout 8 Principle of Operation 9 Optimization Features 12 Overview of the Hydraulic Path 14 Early Maintenance Feedback 20 Instrument Layout 21 Electrical Connections 22
83. ges Inlet Valve Missing 115 Servo Restart Failed 116 Pump Head Missing 117 Index Limit 117 Index Adjustment 118 Index Missing 118 Stroke Length 119 Initialization Failed 119 Electronic fuse of SSV 120 This chapter gives a complete overview over all error messages of the binary 1260 Binary Pump User Manual Error Messages 6 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook 1260 Binary Pump User Manual 101 Error Messages General Error Messages 102 The timeout threshold was exceeded Probable cause 1 The analysis was completed successfully and the timeout function switched off the module as requested 2 Anot ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Shut Down Suggested actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required An external instrument has generated a shut down signal on the re
84. green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running e An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis A red blinking modules with on board LAN or yellow blinking modules without on board LAN indicator indicates that the module is in resident mode e g during update of main firmware A fast red blinking modules with on board LAN or fast yellow blinking modules without on board LAN indicator indicates that the module is in boot loader mode e g during update of main firmware In such a case try to re boot the module or try a cold start 1260 Binary Pump User Manual 125 7 Troubleshooting and Diagnostics User Interfaces 126 Depending on the user interface the available tests vary Some descriptions are only available in the Service Manual Test ChemStation Instant Pilot Agilent LabAdvisor G4208A Pressure Test No Yes Yes Pump Test No No Yes
85. h 86 algae 70 229 alternative seal material 90 ambient operating temperature 41 ambient non operating temperature 41 analog signal output 43 analog signal 27 analog cable 210 apgremote 28 assembly bottle head 186 AUTO mode 19 AUTO pump stroke 13 ball screw drive 14 1260 Binary Pump User Manual battery safety information 226 BCD board 182 cable 215 bench space 40 blank nut 1 16 inch 131 blockage 114 bottle head assembly 186 186 buffer application 70 buffer solutions 8 C cable analog 210 BCD 215 CAN 217 external contact 218 interface 57 LAN 217 remote 212 RS 232 219 cables analog 208 BCD 208 CAN 209 external contact 209 LAN 209 overview 208 remote 208 RS 232 209 CAN cable 217 capillary absorber 15 cleaning 150 Communication settings RS 232C 33 compensation sensor open 105 compensation sensor short 106 compensation compressibility 42 composition accuracy 43 composition precision 43 composition range 43 composition ripple 136 compressibility calibration 122 136 compressibility compensation 42 95 condensation 40 configuration low flowrates 70 connections flow 58 61 control and data evaluation 43 D damaged parts 47 damper when to remove 92 data evaluation and control 43 defect on arrival 46 degreaser spray 163 delay volume 16 delay volume 11 42 92 delivery checklist 47 design 9 diagnostic signals 123 Diagnostic software 127 dimensions 41 dual piston in series de
86. he liquid level in the solvent bottles With total bottle volume and initial filling volume set correctly the pump subtracts the displaced volume continuously from the initial value and reacts before the system runs dry or an analysis is corrupted ve ee i CAUTION The bottle filling feature will fail if multiple channels are fed from one solvent bottle gt In that case implement a minimum pressure limit see Table 16 on page 74 to avoid that the pump runs dry when solvents are empty 1 Open menu Instrument gt More Pump gt Bottle Filling or click on the solvent bottles below the pump icon in the GUI Solvent Bottles Filling X Actual Volume Total Volume amp 10 80 liter 1 00 liter B 10 63 liter 1 00 liter IV Prevent analysis if level falls below 0 100 liter MV Tum pump off if unning out of solvent Cancel Help Figure 23 Bottle Filling Panel 80 1260 Binary Pump User Manual Using the Pump 4 Table 18 Bottle Filling Parameters Parameter Limits Description Total Volume 0 1000L Enter the total capacity of the solvent vessel in this box Mind that the dimension default 0 L is Liters Actual 0 1000L After filling the solvent bottles enter the actual volumes into these boxes The Volume default 0 L Actual Volume must not be larger than the Total Volume of the bottle Prevent default turned off When ticked the pump won t start a new run if the solvent level in one or more anal
87. her modules must have series modules firmware revision A 06 01 B 01 01 or above main and resident Otherwise the communication will not work Conversion to emulation of Special emulation mode firmware is available for conversion to G1367C G1312A A 05 01 03 A 05 06 10 A 05 11 12 and A 06 01 are available as emulation mode firmware If an emulation mode firmware is installed the resident firmware must also be downgraded 1260 Binary Pump User Manual 1260 Binary Pump User Manual J 6 eec 11 e Parts and Materials for Maintenance J Bottle Head Assembly 186 Hydraulic Path with Solvent Selection Valve 188 Hydraulic Path without Solvent Selection Valve 190 Pump Head Assembly Without Seal Wash 192 Pump Head Assembly with Seal Wash Option 194 Outlet Valve 196 Purge Valve Assembly 197 Active Inlet Valve Assembly 198 Accessory Kit 199 Active Seal Wash Option 201 G1316B SL Capillary System Kit 202 Solvent Cabinet 204 Preventive Maintenance Kit G1312 68750 206 This chapter lists all parts and tools that are required for maintenance ee Agilent Technologies 185 11 Parts and Materials for Maintenance Bottle Head Assembly Item p n 1 9301 1450 2 9301 1420 3 G1311 60003 4 5063 6598 5 5063 6599 6 5062 2483 7 5062 8517 8 5041 2168 186 Description Solvent bottle amber Solvent bottle transparent Bottle head assembly Ferrules with lock ring 10x Tube screw 10x Solvent tubing 5 m Inlet filter a
88. inary Pump SL introduces a new compressibility compensation concept The compressibility of a solvent is determined at different pressures between 0 600 bar The pump uses the obtained non linear function to select the correct compressibility value for the actual pump pressure Compressibility data for the most common solvents is readily available in the pump firmware The compensation algorithm is so powerful that the damper and mixer can be removed from the pump flow path at low flow rate while the pressure ripple and composition ripple remain at low levels For method compatibility reasons the legacy compressibility compensation is still available Solvent Compressibility Calibration Unlisted or premixed solvents can be calibrated with the Solvent Compressibility Calibration function For a detailed description see Binary Pump Solvent Calibration on page 136 1260 Binary Pump User Manual 95 5 96 Optimizing Performance Optimization of Legacy Compressibility Settings The compressibility compensation default settings are 50 x 10 bar best for most aqueous solutions for pump head A and 115 x 106 bar to suit organic solvents for pump head B The settings represent average values for aqueous solvents A side and organic solvents B side Therefore it is always recommended to use the aqueous solvent on the A side of the pump and the organic solvent on the B side Under normal conditions the default settings redu
89. ing conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1260 Binary Pump User Manual Introduction to the Binary Pump 1 Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help
90. inlet valve which is processor controlled opened or closed allowing solvent to be drawn into the first pump unit The outlet of the first pump chamber is connected by a 500 uL absorber capillary to the second pump chamber The outlets of the second chambers of both pump channels joined via a small mixing chamber A coiled restriction capillary connects the mixing chamber via a pressure pulse damper a mixer and a pressure sensor to the purge valve assembly The outlet of the purge valve assembly is then connected to the attached chromatographic system When turned on the pump runs through an initialization procedure to determine the upper dead center of the first piston of both pump channels The first piston moves slowly upwards to the mechanical stop of the pump head and from there it moves back a predetermined path length The controller stores this piston position in memory After this initialization the pump starts operation with the set parameters for the two pump channels The active inlet valve is opened and the down moving piston draws solvent into the first pump head At the same time the second piston is moving upwards delivering into the system After a controller defined stroke length depending on the flow rate the drive motors are stopped and the active inlet valve is closed The motor direction is reversed and moves the first piston up until it reaches the stored upper limit and at the same time moving the second piston downwards
91. into the hole on the z panel 1260 Binary Pump User Manual 177 10 Maintenance 3 Guide the wire of the active seal wash assembly through the hole and screw it onto the z panel 4 Guide the wire over the fan and plug the connector onto the mainboard connector P7 Figure 31 Mounting the Seal Wash Pump 5 Replace the foam and top cover 6 Disconnect all capillaries and tubes from the pump head and disconnect the active inlet valve cable 7 Using a 4 mm hexagonal key stepwise loosen and remove the two pump head screws and remove the pump head from the pump drive 178 1260 Binary Pump User Manual Maintenance 10 Installing the Active Seal Wash Option 8 Place the pump head on a flat surface Loosen the lock screw two revolutions and while holding the lower half of the assembly carefully pull the pump head away from the plunger housing Figure 32 Exploded Diagram of the Pump Head with Seal Wash 9 Remove the support rings from the plunger housing and lift the housing away from the plungers 10 Replace the seal wash seals and the film gaskets of the support rings Figure 33 Inserting the active seal wash support rings 1260 Binary Pump User Manual 179 10 Maintenance 180 11 Place the support rings on the plunger housing plungers not installed and snap the pump head and plunger housing together 12 Insert the plungers and carefully press them into the seal 13 Tighten the lock screw 14 Slide the pump he
92. ion to the Binary Pump 1 Table5 8 bit Configuration Switch with on board LAN Mode Function SW 1 SW 2 SW 3 SW 4 SW 5 SW 6 SW7 SW 8 LAN 0 0 Link Configuration Init Mode Selection Auto negotiation 0 xX x x x x 10 MBit half duplex 1 0 0 X X xX 10 MBit full duplex 1 0 1 xX x xX 100 MBit half duplex 1 1 0 x X X 100 MBit full duplex 1 1 1 Xx x xX Bootp x xX x 0 0 0 Bootp amp Store Xx X X 0 0 1 Using Stored x X x 0 1 0 Using Default Xx xX xX 0 1 1 TEST 1 1 System NVRAM Boot Resident System 1 x Revert to Default Data Coldstart X X X 1 Legend 0 switch down 1 switch up x any position When selecting the mode TEST the LAN settings are Auto Negotiation amp Using Stored For explanation of Boot Resident System and Revert to Default Data Coldstart refer to Special Settings on page 35 1260 Binary Pump User Manual 31 1 Introduction to the Binary Pump Setting the 8 bit Configuration Switch without On Board LAN The 8 bit configuration switch is located at the rear of the module Modules that do not have their own LAN interface e g the TCC can be controlled through the LAN interface of another module and a CAN connection to that module Figure 6 Configuration switch settings depend on configured mode All modules without on board LAN e default is ALL DIPS DOWN best settings Bootp mode for LAN e for boot test modes DIPS 1 2 must be UP plus required mode S
93. iption 1 Pump 1 Data System 1 G4208A Instant Pilot 1 Power cord For other cables see text below and Cable Overview on page 208 Locate bench space Provide power connections Unpack the module Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 1260 Binary Pump User Manual 55 3 Installing the Pump Installing the Binary Pump 1 Place the module on the bench in a horizontal position 2 Ensure the power switch on the front of the pump is OFF switch stands out Status Indicator wt SKK atmaca a Patos Power switch Serial number Figure 13 Front of Binary Pump 3 At the rear
94. ixer are disconnected while the other part is still in line are not supported by Agilent Technologies Tools required Wrench 1 4 5 16 inch Wrench 14 mm Hex driver open 1 4 inch Preparations Flush the system water if buffers were used otherwise isopropanol Turn the flow off 1 Remove the front cover by pressing the clip fasteneron 2 Use the 1 4 inch hex driver to remove fitting B from port 2 both sides of the cover of the pressure sensor 1260 Binary Pump User Manual 93 5 Optimizing Performance When to Remove Damper and Mixer 3 Fold capillary end B away It remains 4 Connect fitting A to port 2 of the pressure sensor Seal unconnected Disconnect fitting A from outlet 7 of the port 7 of the mixer with a plastic blank nut mixer ae as Fe me 94 1260 Binary Pump User Manual Optimizing Performance 5 How to Optimize the Compressibility Compensation Setting When a solvent is metered at ambient pressure and compressed to a higher pressure the volume decreases This is due to an effect known as solvent compressibility Solvent compressibility is a non linear function of pressure and temperature It is unique to every solvent In order to deliver the desired flow accurately at all pressures Agilent pumps use a compressibility compensation Usually an average compressibility value for the solvent is used across the whole pressure range of the pump The G1312B B
95. k Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 1260 Binary Pump User Manual 213 12 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Pin Universal Pin Agilent Signal Name Active module TTL 1 White Digital ground A O 1 2 B oo Brown Prepare run Low o0 oo og 3 Gray Start Low KEY coal oo BG 4 Blue Shut down Low o0 am 5 Pink Not connected oOo 0 ji 6 Yellow Power on High O 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 214 1260 Binary Pump User Manual BCD Cables Identifying Cables 12 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connec ted to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 Violet 2 BCD7 80 4 Blue 3 BCD 6 40 m Yellow 4 BCD 4 10 SS Black 5 BCD 0 1 ay Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 1260 Binary Pump User Manu 215 12 Identifying Cables Agilent Module to 3396 Integrators
96. lection valve and empty the tubes into the H i E bottles Place the bottles back into the solvent cabinet i ji S RN 174 1260 Binary Pump User Manual Maintenance 10 Exchanging the Solvent Selection Valve 3 Using a Pozidriv screwdriver 1 loosen the holding screws of the valve holder 4 Carefully pull the valve holder out and disconnect the valve cable at the connector 6 Tighten the screws of the valve holder 1260 Binary Pump User Manual 175 10 Maintenance Exchanging the Solvent Selection Valve 7 Reconnect all tubings to the solvent selection valve NOTE After an exchange of the valve it may be required to pump several mL of solvent before the flow stabilizes at a pressure ripple as low as it used to be when the system was still working properly 176 1260 Binary Pump User Manual Maintenance 10 Installing the Active Seal Wash Option When When using concentrated buffers gt 0 1 M Tools required 4 mm hexagonal key Screwdriver Pozidrive 1 Parts required p n Description 1 G1312 68721 Active Seal Wash Option kit Preparations Switch off the pump at the main power switch Remove the front cover Remove the top cover and foam 1 By using a screwdriver remove the metal plug in the z panel A j dor Figure 30 Removing the Metal Plug from the Z Plane 2 Insert the socket delivered with the Seal Wash pump assembly
97. lve 0 Failed valve A1 Valve 1 Failed valve A2 Valve 2 Failed valve B2 Valve 3 Failed valve B1 Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative One of the solvent selection valves in the module failed to switch correctly The processor monitors the valve voltage before and after each switching cycle If the voltages are outside expected limits the error message is generated Probable cause 1 Solvent selection valve disconnected 2 Connection cable inside instrument not connected 3 Connection cable inside instrument defective 4 Solvent selection valve defective Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Exchange the solvent selection valve 1260 Binary Pump User Manual Error Messages 6 Missing Pressure Reading The pressure readings read by the pump ADC analog digital converter are missing The ADC reads the pressure signal of from the damper every Ims If the readings are missing for longer than 10 seconds the error message is generated Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service rep
98. m Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail 1 Select the pressure test from the test selection menu 2 Start the test and follow the instructions Make sure to release the pressure by opening the purge valve when the test has finished completed Otherwise the pump may generate an overpressure error 1260 Binary Pump User Manual 131 132 Tests Functions and Calibration Evaluating the Results The sum of all leaks between the pump and the blank nut will add up to the total leak rate Note that small leaks may cause the test to fail but solvent may not be seen leaking from a module Please notice the difference between an error in the test and a failure of the test An error is caused by the abnormal termination during the operation of the test whereas a failure of a test indicates that the test results were not within the specified limits If the pressure test fails e Ensure all fittings between the pump and the blank nut are tight Repeat the pressure test Often it is only a damaged blank nut itself poorly shaped from overtightening that causes the test to fail Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened e Ifthe test fails again insert the blank nut at the outlet of the previous module in the stack e g autosampler port 6 of the injection valve
99. margin of 10 as new EMF limits Reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed at the appropriate time providing a reminder that maintenance needs to be scheduled 1260 Binary Pump User Manual 83 4 Using the Pump Early Maintenance Feedback EMF 84 1260 Binary Pump User Manual 1260 Binary Pump User Manual 5 Optimizing Performance Prevent Blocking of Solvent Filters 86 Checking the Solvent Filters 86 Cleaning the Solvent Filters 87 When to Use a Vacuum Degasser 88 Operational Hints for the Vacuum Degasser 88 When to Use the Active Seal Wash Option 89 When to Use Alternative Seals 90 When to Use the Low volume mixer 91 When to Remove Damper and Mixer 92 Convert the Binary Pump to Low Delay Volume Mode 93 How to Optimize the Compressibility Compensation Setting 95 Solvent Compressibility Calibration 95 Optimization of Legacy Compressibility Settings 96 This chapter gives information on how to optimize the performance of the Binary Pump under special operational conditions ot Agilent Technologies 85 5 Optimizing Performance Prevent Blocking of Solvent Filters 86 Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lif
100. mote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition Check the vacuum degasser for an error condition Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built in 1260 Binary Pump User Manual Remote Timeout Error Messages 6 A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective component
101. mp parts mainboard pump drive The test allows to define which pump head will be calibrated Results of the pump elasticity calibration rely on known compressibility parameters for pure water If the water is not HPLC grade not well degassed or degasser and pump are not flushed properly the pump elasticity calibration will fail The pump elasticity calibration has to be performed for each pump head individually Incorrect pump elasticity calibration Solvent compressibility calibrations acquired with a miscalibrated pump will work but they are not transferable to other pumps A correct pump elasticity calibration is an essential prerequisite for successful solvent compressibility calibrations gt Calibrate the pump elasticity correctly 138 1260 Binary Pump User Manual Tests Functions and Calibration 8 Running the Pump Elasticity Calibration Running the Pump Elasticity Calibration from the Agilent LC Diagnostic Software When The initial calibration is done at the factory It only needs to be repeated after replacement of major pump parts mainboard pump drive Tools required Wrench 1 4 5 16 inch Parts required Description G1312 67500 Calibration capillary assembly Preparations Place all bottle heads in to a bottle of HPLC grade water Make absolutely sure that the pump is very thoroughly flushed with the solvent to be calibrated before starting the procedure Any trace of other solvents or the smallest air
102. n board Suggested actions Restart the module If the error message appears again exchange the active inlet valve Please contact your Agilent service representative Please contact your Agilent service representative 1260 Binary Pump User Manual Error Messages 6 Temperature Out of Range Temperature Out of Range 0 Pump channel A Temperature Out of Range 1 Pump channel B One of the temperature sensor readings in the motor drive circuit are out of range The values supplied to the ADC by the hybrid sensors must be between 0 5 V and 4 3 V If the values are outside this range the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Temperature Limit Exceeded Temperature Limit Exceeded 0 Pump channel A Temperature Limit Exceeded 1 Pump channel B The temperature of one of the motor drive circuits is too high The processor continually monitors the temperature of the drive circuits on the main board If excessive current is being drawn for long periods the temperature of the circuits increases If the temperature exceeds the upper limit the error message is generated Probable cause Suggested actions 1 High friction partial mechanical blockage in Ensure the capillaries and frits between the the pump drive assembly pump head and damper inlet are free from blockage 2 Partial blockage of the flowpath in front of Ensure the o
103. nalysis Priming is usually done by pumping at a high flow rate 8 5 mL min Alternatively a syringe can be used to draw the solvent through the empty degasser if the pump does not aspirate the solvent by itself For details see Initial Priming on page 64 For more information see the Agilent 1260 Infinity Standard Degasser User Manual p n G1322 90012 88 1260 Binary Pump User Manual Optimizing Performance 5 When to Use the Active Seal Wash Option Concentrated buffer solutions will reduce the lifetime of the seals and plungers in your binary pump The active seal wash option allows to maintain the seal lifetime by flushing the low pressure side of the seals with a wash solvent The seal wash option is strongly recommended if buffer concentrations of 0 1 M or higher are used regularly with the pump The active seal wash option kit can be ordered by quoting Active Seal Wash Option kit p n G1312 68721 The seal wash option comprises a peristaltic pump secondary seals gaskets seal keepers and tubing for both pump heads A bottle of premixed water isopropanol 90 10 vol is placed in the solvent cabinet and connected to the peristaltic pump as described in the technical note that comes with the active seal wash kit Always use a mixture of HPLC grade water 90 and isopropanol 10 as wash solvent This mixture prevents bacteria growth in the wash bottle and reduces the surface tension of the water The operation
104. nce Specifications Table 14 Performance Specifications of the Agilent 1260 Infinity Binary Pump G1312B Type Specification Comments Hydraulic system Setable flow range Flow range Flow precision Flow accuracy Pressure Pressure pulsation Compressibility compensation Recommended pH range Gradient formation Delay volume 42 Two dual piston in series pumps with proprietary servo controlled variable stroke drive floating piston design Dual piston in series pump with proprietary servo controlled variable stroke drive floating pistons 0 05 5 0 mL min lt 0 07 RSD or lt 0 02 min SD whatever is greater 1 or 10 pL min what ever is greater pumping degassed H20 at 10 MPa Operating range 0 60 MPa 0 600 bar 0 8700 psi up to 5 mL min lt 2 amplitude typically lt 1 3 or lt 3 bar at 1 mL min isopropanol at all pressures gt 10 bar 147 psi Low delay volume configuration lt 5 amplitude typically lt 2 Automatic pre defined based on mobile phase compressibility 1 0 12 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel High pressure binary mixing Standard delay volume configuration 600 800 uL dependent on back pressure includes 400 uL mixer Low delay volume configuration 120 uL based on retention time at constant room temperature measured with water at 1 mL min water measured
105. nd 1 means that the switch is up 1260 Binary Pump User Manual 33 1 34 Introduction to the Binary Pump Table 8 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table9 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 10 Parity Settings without on board LAN Switches Parity 7 8 0 0 No Parity 1 0 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity 1260 Binary Pump User Manual Introduction to the Binary Pump 1 Special Settings The special settings are required for specific actions normally in a service case The tables include both settings for modules with on board LAN and without on board LAN They are identified as LAN and no LAN Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loa
106. nerates the smallest pressure ripple is the optimum value for your solvent composition 6 Repeat step 1 through step 5 for the B channel of your binary pump 1260 Binary Pump User Manual Optimizing Performance 5 Table 19 Solvent Compressibility Solvent pure Compressibility 10 bar Acetone 126 Acetonitrile 115 Benzene 95 Carbon tetrachloride 110 Chloroform 100 Cyclohexane 118 Ethanol 114 Ethyl acetate 104 Heptane 120 Hexane 150 Isobutanol 100 lsopropanol 100 Methanol 120 1 Propanol 100 Toluene 87 Water 46 1260 Binary Pump User Manual 97 5 Optimizing Performance How to Optimize the Compressibility Compensation Setting 98 1260 Binary Pump User Manual 1260 Binary Pump User Manual 6 Error Messages What Are Error Messages 101 General Error Messages 102 Timeout 102 Shut Down 102 Remote Timeout 103 Synchronization Lost 103 Leak 104 Leak Sensor Open 104 Leak Sensor Short 105 Compensation Sensor Open 105 Compensation Sensor Short 106 Fan Failed 106 OpenCover 107 Module Error Messages 108 Restart Without Cover 108 Zero Solvent Counter 108 Pressure Above Upper Limit 109 Pressure Below Lower Limit 109 Pressure Signal Missing 110 Valve Failed 110 Missing Pressure Reading 111 Pump Configuration 111 Selection Valve Fuse 112 Inlet Valve Fuse 112 Temperature Out of Range 113 Temperature Limit Exceeded 113 Motor Drive Power 114 Encoder Missing 115 App Agilent Technologies 99 100 Error Messa
107. of the module move the security lever to its maximum right position 4 Connect the power cable to the power connector at the rear of the module The security lever will prevent that the cover is opened while the power cord is connected to the module 56 1260 Binary Pump User Manual Installing the Pump 3 Installing the Binary Pump 5 Connect the required interface cables to the rear of the module Configuration switch i Slot for interface board _ _ __ lt __ RS232 Remote Analog output CAN Bus Power Plug Security Lever Figure 14 Rear of the Binary Pump 6 Connect the capillary solvent tubes and waste tubings see Flow Connections with Solvent Selection Valve on page 58 or Flow Connections without Solvent Selection Valve on page 61 7 Press the power switch to turn on the module NOTE The power switch stays pressed in and a green indicator lamp in the power switch is on when the module is turned on When the line power switch stands out and the green light is off the module is turned off 8 Purge the pump see Initial Priming on page 64 1260 Binary Pump User Manual 57 3 Installing the Pump Flow Connections with Solvent Selection Valve Parts required p n Description 1 Other modules 1 G1312 68755 Accessory Kit 1 G1312 68765 Accessory Kit 2 wrenches 1 4 5 16 inch for capillary connecti
108. olvent vendor especially when toxic or hazardous solvents are used 1260 Binary Pump User Manual 61 3 62 Installing the Pump 1 Remove the front cover by pressing the snap fasteners on both sides Figure 17 Removing the Front Cover Place the solvent cabinet on top of the module Place the bottles into the solvent cabinet and place a bottle head assembly into each bottle Connect the solvent tubes from the bottle head assemblies to the inlet adapters of the active inlet valves Fix the tubes in the clips of solvent cabinet and binary pump Hold the waste tubing with a piece of sandpaper and push it onto the purge valve outlet Place the end into your waste system If the pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system Connect the pump outlet capillary pump to injection device to the outlet of the purge valve 1260 Binary Pump User Manual Installing the Pump 3 Flow Connections without Solvent Selection Valve 8 Purge your system before first use see Initial Priming on page 64 Bottle head assembly H piee e Tube clip not visible T H J F di Pressure sensor fea al tx Y Static Mixer p S Purge valve BAR Se Pump head A Lh a Oe Pump head B Sw EE Adapter iar a ae j Active inlet valve A a I HH Active inlet valve B
109. ons Preparations Pump is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can bear health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 58 1260 Binary Pump User Manual Installing the Pump 3 1 Remove the front cover by pressing the snap fasteners on both sides Figure 15 Removing the Front Cover N If available place the online degasser on top of the pump amp Place the solvent cabinet on top of the module A Set the four bottles into the solvent cabinet and screw a bottle head assembly onto each bottle 5 Connect the solvent tubes from the bottle head assemblies to the inlet connectors Al A2 B1 and B2 of the solvent selection valve Make sure to use the brown bottle for the aqueous solvent usually channel A1 6 Label the tubes accordingly using the supplied stickers and fix the tubes in the clips of solvent cabinet and binary pump 7 Hold the waste tubing with a piece of sandpaper and push it onto the purge valve outlet Place the end into your waste system 8 Ifthe pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the corrugat
110. or monitors the pump pressure A purge valve with integrated PTFE frit is fitted to the pump outlet for convenient priming of the pumping system Damper Purge valve Mixer Pump outlet J Inlet valve Mixing Inlet valve gt Chamber s from from solvent Seals solvent bottle A bottle B Pistons Pistons Pump head A Pump head B Figure 1 The Hydraulic Path of the Binary Pump with Damper and Mixer 1260 Binary Pump User Manual 9 1 Introduction to the Binary Pump Instrument and Op eration Damper and mixer can be bypassed for lowest delay volume of the binary pump This configuration is recommended for low flow rate applications with steep gradients see the Rapid Resolution System Manual Figure 1 on page 9 illustrates the flow path in low delay volume mode For instructions on how to change between the two configurations see Convert the Binary Pump to Low Delay Volume Mode on page 93 NOTE Bypassing the mixer while the damper remains in line is not a supported configuration and abate may lead to undesired behavior of the binary pump Purge valve eo Pump outlet Pressuresensor to waste Outlet valve Inlet valve Inlet valve _ 2 from solvent from solvent bottle A bottle B Pistons Pistons Pump head A Pump head B Figure 2 The Hydraulic Path of the Binary Pump with Bypassed Damper and Mixer 10 1260
111. or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false e fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal 1260 Binary Pump User Manual Introduction to the Binary Pump 1 Table4 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for
112. ork but they are not transferable to other pumps A correct pump elasticity calibration is an essential prerequisite for successful solvent compressibility calibrations gt Calibrate the pump elasticity correctly 18 How Does Variable Stroke Volume Work The smaller the solvent volume in the pump chamber is the faster it can be recompressed to operating pressure The binary pump allows to manually or automatically adjust the pump stroke volume of the first piston in the range of 20 100 uL Due to the compression of the solvent volume in the first pump chamber each piston stroke of the pump will generate a small pressure pulsation influencing the flow ripple of the pump The amplitude of the pressure pulsation is mainly dependent on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes generate less pressure pulsation than larger stroke volumes at the same flow rate In addition the frequency of the pressure pulsation will be higher This will decrease the influence of flow pulsations on quantitative results In gradient mode smaller stroke volume results in less flow ripple and improves the composition ripple 1260 Binary Pump User Manual Introduction to the Binary Pump 1 The binary pump uses a processor controlled ball screw system to drive its pistons The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow
113. osampler column frits and capillaries with small internal diameters e g 50 pm ID 3 Blockage in the flowpath after the damper Check for blockage in the flowpath 4 Defective damper Please contact your Agilent service representative 5 Defective main board Please contact your Agilent service representative Pressure Below Lower Limit The system pressure has fallen below the lower pressure limit Probable cause Suggested actions 1 Lower pressure limit set too high Ensure the lower pressure limit is set to a value suitable for the analysis 2 Air bubbles in the mobile phase Ensure solvents are degassed Purge the module Ensure solvent inlet filters are not blocked 3 Leak Inspect the pump head capillaries and fittings for signs of a leak Purge the module Run a pressure test to determine whether the seals or other module components are defective 4 Defective damper Please contact your Agilent service representative 5 Defective main board Please contact your Agilent service representative 1260 Binary Pump User Manual 109 110 Error Messages Pressure Signal Missing The pressure signal of the damper is missing The pressure signal of the damper must be within a specific voltage range If the pressure signal is missing the processor detects a voltage of approximately 120mV across the damper connector Probable cause 1 Damper disconnected 2 Defective damper Valve Failed Va
114. ovides the following EMF counters liquimeter pump A seal wear pump A liquimeter pump B and e seal wear pump B Liquimeters The liquimeters display the total volume of solvent pumped by the left and right pump heads since the last reset of the counters Both liquimeters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed 1260 Binary Pump User Manual Usingthe Pump 4 Seal Wear Counters The seal wear counters display a value derived from pressure and flow both contribute to seal wear The values increment with pump usage until the counters are reset after seal maintenance Both seal wear counters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed Using the EMF Counters The user settable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The wear of pump components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Note the pumped volumes and the seal wear values of both pump heads when symptoms of excessive seal wear are observed Perform pump maintenance and enter the noted EMF values minus a safety
115. p User Manual Appendix 13 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 1260 Binary Pump User Manual 227 13 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 228 1260 Binary Pump User Manual Appendix 13 Solvent Information Observe the following recommendations on the use of solvents e Brown glass ware can avoid growth of algae e Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 2 um filters e Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid and nitri
116. page 154 2 Outlet valve Exchanging the Outlet Valve on page 169 3 Passive inlet valve 4 Delay volume reduction ol Pump head Removing the Pump Head Assembly on page 156 Solvent selection valve Exchanging the Solvent Selection Valve on page 174 1260 Binary Pump User Manual 151 10 Maintenance Maintenance Procedures The procedures described in this section can be done with the binary pump in place in the system stack Table 23 Maintenance Procedures Procedure Typical Frequency Notes Exchanging the Purge Valve Frit or the Yearly or if the fritshows A pressure drop of gt 10 bar across the frit Purge Valve on page 154 indication of 5 mL min H20 with purge valve open indicates contamination or blockage blockage Removing the Pump Head Assembly on During yearly Necessary to get access to pump seals and page 156 maintenance plungers Disassembling the pump head on page 158 During yearly maintenance Exchanging the Pump Seals on page 159 Yearly or if pump performance indicates seal wear Exchanging the Pistons on page 162 If scratched or if dents are visible Exchanging the Wash Seals on page 163 Yearly Necessary to get access to pump seals and plungers Leaks at lower pump head side unstable retention times pressure ripple unstable run pump test for verification Seal life time shorter than normally expected check plungers while changing the s
117. piston 1 into pump chamber 2 Without any compensation the delivered volume at operating pressure would be too low In addition it would take a noticeable amount of time to recompress the solvent to operating pressure During this time frame no 1260 Binary Pump User Manual 17 1 Introduction to the Binary Pump CAUTION solvent would be delivered into the system and as a result a high pressure fluctuation known as pressure ripple would be observed When both solvent compressibility at the current operating pressure and pump elasticity are known the pump can automatically correct for the missing volume by drawing the appropriate larger solvent volume at ambient pressure and speed up the piston during the recompression phase in the first pump chamber As a result the pump delivers the accurate volume with any calibrated solvent at any pressure at a greatly reduced pressure ripple For applications that require lowest transition volume of the pump damper and mixer can be bypassed For compatibility with older methods from G1312A binary pumps the old one point compressibility compensation is available too However since the compressibility is a non linear function one single compressibility value per solvent will only give good results at one particular pressure which is at 200 bar for the G1312A binary pump Incorrect pump elasticity calibration Solvent compressibility calibrations acquired with a miscalibrated pump will w
118. pump head 156 repair procedures 152 repairs definition of 148 introduction 148 replacing firmware 183 183 warnings and cautions 148 233 Index restart without cover 108 results pressure test 132 pressure test 135 ripple composition 136 pressure 136 RS 232C cable 219 communication settings 33 S safety class 222 safety information lithium batteries 226 safety general information 222 standards 41 symbols 224 sapphire piston 14 screwdriver pozidriv 1 156 screwdriver pozidriv 1 163 174 screwdriver flat head 163 seal wash wash solvent 89 when touse 89 seal wear counter 145 seal wear counter 160 seal wear 83 seals alternative material 90 normal phase 90 security lever 56 selection valve failed 110 selection valve fuse 112 serial number information 23 servo restart failed 116 setable flowrange 42 234 shut down 102 sieve 153 169 simple repair procedures 152 site requirements 37 power cords 39 snap fastener 59 62 solubility gas 70 solvent cabinet 59 solvent compressibility calibration 136 137 solvent filters cleaning 87 solvent inlet filter 70 solvent selection valve 8 174 solvent cabinet 70 solvent filters checking 86 prevent blocking 86 solvent selection valve 58 solvents 229 sonic bath 169 sound emission 228 special interfaces 29 special settings boot resident 35 forced cold start 36 specification physical 41 specifications analog signal output 43 communications 4
119. r gives information about the preferred stack setup for your system and the installation of your binary pump ott Agilent Technologies 45 3 Installing the Pump Unpacking the Binary Pump Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 46 1260 Binary Pump User Manual Delivery Checklist Installing the Pump 3 Compare the delivery checklist with the contents of the shipping boxes to ensure completeness of the shipment The contents lists is shown below For parts identification check the illustrated parts breakdown in Parts and Materials for Maintenance on page 185 Please report missing or damaged parts to your local Agilent Technologies sales and service office p n G1312 67500 G1312 68700 0100 1681 9301 0411 5067 1531 5067 1532 9301 1450 9301 1420 827700 902 827975 902 927975 902 G1312 68755 G1312 68765 1260 Binary P
120. r message to be generated Probable cause Suggested actions 1 Defective pump drive assembly Please contact your Agilent service representative Initialization Failed Initialization Failed Pump channel A B Initialization Failed Pump channel B The module failed to initialize successfully within the maximum time window A maximum time is assigned for the complete pump initialization cycle If the time is exceeded before initialization is complete the error message is generated Probable cause Suggested actions 1 Blocked passive inlet valve Exchange the inlet valve 2 Defective pump drive assembly Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 1260 Binary Pump User Manual 119 6 Error Messages Electronic fuse of SSV The electronic fuse protecting the solvent selection valve electronics has blown Probable cause Suggested actions 1 Recoverable error of the SSV electronic Restart module the electronic fuse can recover If not contact Agilent service 2 Short cut of SSV cable Replace cable between board and SSV 120 1260 Binary Pump User Manual 1260 Binary Pump User Manual 7 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 122 Status Indicators 124 Power Supply Indicator 124 Module Status Indicator 125 User Interfaces 126 Agilent Lab Advisor Software 127 Overview of the troubleshoo
121. rates use a higher stroke volume The stroke volume for the pump is by default set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended 1260 Binary Pump User Manual 19 1 Introduction to the Binary Pump Early Maintenance Feedback 20 Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operat
122. resentative Pump Configuration At switch on the pump has recognized a new pump configuration The binary pump is assigned its configuration at the factory If the active inlet valve and pump encoder of channel B are disconnected and the binary pump is rebooted the error message is generated Probable cause Suggested actions 1 Active inlet valve and pump encoder of Reconnect the active inlet valve and pump channel B disconnected encoder of channel B 1260 Binary Pump User Manual 111 112 Error Messages Selectio Inlet Val n Valve Fuse Valve Fuse 0 Channels Al and A2 Valve Fuse 1 Channels B1 and B2 One of the solvent selection valves in the module has drawn excessive current causing the selection valve electronic fuse to open Probable cause 1 Defective solvent selection valve 2 Defective connection cable front panel to main board 3 Defective main board ve Fuse Inlet Valve Fuse 0 Pump channel A Inlet Valve Fuse 1 Pump channel B Suggested actions Restart the pump If the error message appears again exchange the solvent selection valve Please contact your Agilent service representative Please contact your Agilent service representative One of the active inlet valves in the module has drawn excessive current causing the inlet valve electronic fuse to open Probable cause 1 Defective active inlet valve 2 Defective connection cable front panel to main board 3 Defective mai
123. residual isopropanol Capillaries and filter may be cloged by precipitating salt gt Don t perform steps 5 to 7 for channels run with aqueous buffer as solvent Replace the solvent bottle by a bottle of isopropanol Flush the channel at a flow rate suitable for the installed tubing typically 3 5 mL min for 5 min Swap the bottle of isopropanol with a bottle of solvent for your application Repeat steps 1 to 7 for the other channel s of the pump Install the desired column set the required composition and flow rate for your application and equilibrate the system for approx 10 minutes prior to starting a run 1260 Binary Pump User Manual 67 3 68 Installing the Pump Table 15 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of When switching between lsopropanol the system reverse phase and normal Miscible with almost all phase both times solvents After an installation To clean the system when using buffers After changing aqueous solvents After the installation of normal phase seals P N 0905 1420 Ethanol or methanol HPLC grade water HPLC grade water Hexane 5 isopropanol Alternative to isopropanol second choice if no isopropanol is available Best solvent to re dissolve buffer crystals Best solvent to re dissolve buffer crystals Good wetting properties 1260 Binary Pump User Manu
124. reversed and the piston 1 now compresses the solvent until the operating pressure of the HPLC system is reached The outlet ball valve opens and solvent is pumped by piston 1 into pump chamber 2 Due to two factors the solvent volume that is delivered into the system at high pressure is smaller than it is supposed to be 1 The solvent is compressible 2 The pump has a certain elasticity which causes it s internal volume to increase with pressure In order to compensate for these two influences their absolute value must be known Since the properties of pure water are very well documented it s compressibility can be preset When pumping water any deviations from the theoretical pressure profile during solvent recompression are caused by the elasticity of the pump 1260 Binary Pump User Manual Introduction to the Binary Pump 1 The Pump Elasticity Calibrationcalculates correction factors to compensate for the individual elasticity of the pump that was calibrated The elasticity is different for every pump and may change with the replacement of parts in the flow path e g pump seals All binary pumps are elasticity calibrated at the factory and require recalibration only after preventive maintenance or major repairs to the flow path Replacement of capillaries or PTFE frits are not considered a major repair How Does Compressibility Compensation Work Although the compressibility of liquids is orders of magnitude lower then th
125. rge valve during initialization The volume one pump piston delivers per stroke Generally a smaller stroke volume results in lower pump ripple The Auto setting adjusts the strokes dynamically to the lowest possible value The strokes can be set individually for pump heads A and B It is highly recommended to tick the Use enhanced compressibility calibration box This forces the pump to use either stored solvent compressibility data or user generated compressibility parameters from solvent compressibility calibrations For legacy support the solvent compressibility can still be set manually for each channel when the box is unticked 78 1260 Binary Pump User Manual Using the Pump 4 Setting up the Pump with Agilent ChemStation Data Curves The binary pump provides the possibility to store operational data in the data file of the Agilent data system Solvent percentage for each channel pump flow and pressure are stored when the respective boxes are ticked Access the Pump Data Curves panel either from the Instrument gt More Pump gt Data Curves menu or by left clicking the pump icon in the GUI Pump Data Curves Figure 22 Data Curves Panel NOTE The pressure data curve is generated from the pressure sensor readings while A B and flow are calculated from the method settings of the pump 1260 Binary Pump User Manual 79 4 Using the Pump Bottle Filling The pump offers a powerful feature to monitor t
126. rument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits are exceeded The visual feedback in the user interface indicates when maintenance procedures should be scheduled EMF Counters The Binary Pump SL provides a series of EMF counters for the left and right pump heads Each counter increments with pump use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The Binary Pump SL provides the following EMF counters liquimeter pump A seal wear pump A liquimeter pump B and seal wear pump B Liquimeters The liquimeters display the total volume of solvent pumped by the left and right pump heads since the last reset of the counters Both liquimeters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed 144 1260 Binary Pump User Manual Diagnostic Signals 9 Seal Wear Counters The seal wear counters display a value derived from pressure and pumped volume both contribute to seal wear The values increment with pump usage until the counters are reset after seal maintenance Both seal wear counters can be assigned an EMF maximum limit When the limit is exceed
127. ry 90 mm 0 12 mm i d m f SST capillary 70 mm 0 12 mm i d male female SST capillary 50 mm 0 12 mm i d male female SST Capillary 170 mm 0 12 mm i d m f SST Capillary 500 mm 0 12 mm i d m m SST Capillary 500 mm 0 12 mm i d m m 203 11 Parts and Materials for Maintenance DOIVE GADINCGE Solvent Cabinet Item p n Description 1 5065 9981 Solvent cabinet including all plastic parts 2 5042 8901 Name plate 3 5065 9954 Front panel solvent cabinet 4 5042 8907 Leak pan solvent cabinet 5 9301 1450 Solvent bottle amber 9301 1420 Solvent bottle transparent 7 G1311 60003 Bottle head assembly Figure 43 Solvent Cabinet Parts 1 204 1260 Binary Pump User Manual Parts and Materials for Maintenance 11 Solvent Cabinet cap l Figure 44 Solvent Cabinet Parts 2 1260 Binary Pump User Manual 205 11 Parts and Materials for Maintenance Preventive Maintenance Kit G1312 68750 206 Description Pump seal Wash seal Gold seal outlet Cap Gasket wash seal PTFE frits o O wo A 1260 Binary Pump User Manual 1260 Binary Pump User Manual 6 ee 12 e Identifying Cables J Cable Overview 208 Analog Cables 210 Remote Cables 212 BCD Cables 215 CAN LAN Cables 217 External Contact Cable 218 RS 232 Cables 219 Agilent 1200 Module to Printer 220 This chapter provides information on cables Agg Agilent Technologies 207 12 Identifying Cables Cable Overvi
128. s in the instrument showing the not ready condition Synchronization Lost Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause 1 CAN cable disconnected 2 Defective CAN cable 3 Defective main board in another module 1260 Binary Pump User Manual Suggested actions Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly Exchange the CAN cable Switch off the system Restart the system and determine which module or modules are not recognized by the system 103 104 Error Messages Leak A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on
129. seal for damage Check the gold seal It should be exchanged when heavily deformed Inspect the cap and replace it with a new one if cracks are visible 4 Reinstall the outlet valve and tighten it 1260 Binary Pump User Manual 169 10 Maintenance Exchanging the Outlet Valve 5 Reconnect the valve capillary Absorber capillary Outlet valve 170 1260 Binary Pump User Manual Maintenance 10 Installation of the Solvent Selection Valve Upgrade Kit When Tools required Parts required Preparations A solvent selection valve allows you choosing between 4 different solvents that can be used with a binary pump The valve switches between two solvents Al and A2 for channel A of the left pump head and two solvents B1 and B2 for channel B of the right pump head Applicable modules This kit is compatible to the 1260 Binary Pumps G1312B and G1312C Screwdriver Pozidriv 1 Description G1381 60000 Solvent Selection Valve Upgrade Kit If required remove solvent tubes from the inlet valves The figures below show a Binary Pump G1312B The kit can be used similarly for the Binary Pump G1312C Figures show passive inlet valves If the pump uses active inlet valves they can be used similarly 1260 Binary Pump User Manual 171 10 Maintenance Installation of the Solvent Selection Valve Upgrade Kit 1 Remove the front cover sheet by removing both screws 2 Plug in the connector of the solvent selection valve Connector
130. sign 9 231 Index E early maintenance feedback 82 elasticity calibration 123 electrical connections descriptions of 22 electronic waste 225 electrostatic discharge ESD 182 EMF counter 144 EMF flag 144 EMF counters maximum limit 82 liquimeter 82 sealwear 83 setting limits 83 usage 83 EMF flag 82 EMF limits 83 EMF early maintenance feedback 20 encoder missing 115 error messages fan failed 106 overpressure 131 135 137 139 error messages compensation sensor open 105 compensation sensor short 106 encoder missing 115 ignition without cover 107 107 index adjustment 118 index limit 117 index missing 118 initialization failed 119 inlet valve fuse 112 inlet valve missing 115 eak sensor open 104 eak sensor short 105 eak 104 missing pressure reading 111 motor drive power 114 pressure above upper limit 109 232 pressure below lower limit 109 pump head missing 117 remote timeout 103 selection valve failed 110 selection valve fuse 112 servo restart failed 116 shut down 102 stroke length 119 synchronization lost 103 temperature limit exceeded 113 temperature out of range 113 timeout 102 error zero solvent counter 108 exchanging inlet valve 153 167 interface board 182 outlet valve sieve 169 outlet valve sieve 153 outlet valve 153 169 pistons 152 162 pump seals 159 pump seals 152 purge valve frit 153 153 purge valve 153 153 solvent selection valve 174 wash seals 163 177 wash seals 152
131. t you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product s Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1260 Binary Pump User Manual 225 13 Appendix Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 226 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1260 Binary Pum
132. terface to other Agilent products e Analog output connector s for signal output 1260 Binary Pump User Manual 25 1 26 Introduction to the Binary Pump Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369A B LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a connected PC with the appropriate control software If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for e 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable 1260 Binary Pump User Manual Introduction to the Binar
133. the inlet valve and remove the valve from the pump head 1260 Binary Pump User Manual 167 10 Maintenance 4 Screw the valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight 5 Using the 14 mm wrench tighten the valve do not over tighten 6 Reinstall the front cover After an exchange of the valve it may be required to pump several mL of the solvent used in the current application before the flow stabilizes at a pressure ripple as low as it used to be when the system was still working properly 168 1260 Binary Pump User Manual Maintenance 10 Exchanging the Outlet Valve When Tools required Parts required Preparations if leaking internally tools wrench 1 4 5 16 inchWrench 1 4 inch Wrench 14 mm Description G1312 60067 Outlet valve complete Switch off the pump at the main power switch Before exchanging the outlet valve you can try to clean it in an ultrasonic bath Remove the gold seal and put the plastic cap back on to protect the sealing surface from scratches Place the valve in upright position sitting on the plastic cap in a small beaker with premixed water isopropanol 50 50 Sonicate for 5 to 10 minutes Replace the gold seal 1 Using a 1 4 inch wrench disconnect the valve capillary from the outlet valve 2 Unscrew the valve with the 14 mm wrench and remove it from the pump body 3 In case you are only sonicating the valve check the plastic cap and the gold
134. the main power switch Make sure that the pump head is not removed This may damage the pump drive gt Never start the pump when the pump head is removed 156 Both pump head assemblies use the same internal components In addition pump head A is fitted with the purge valve The following procedure describes the removal and disassembly of pump head A left For pump head B right proceed in the same way and skip steps that deal with the purge valve 1 Remove the front cover 2 2 Disconnect the capillaries at the back of the purge valve holder the pump head adapter and the tube at the inlet valve Beware of leaking solvents 1260 Binary Pump User Manual Maintenance 10 Removing the Pump Head Assembly 3 Using a 4 mm hexagonal key stepwise loosen and remove the two pump head screws Pump head screws 4 Using a 3 mm hexagonal key loosen the purge valve holder and remove it from pump head body 1260 Binary Pump User Manual 157 10 Maintenance embling the pump head Disassembling the pump head Place the pump head with the front face onto a bench Open the two hex screws on the rear side with a 3 mm hex driver Open the PEEK lock screw two to three turns Aa QO N Pull the spring housing up and remove it from the pump head Spring housing lt 5 Giese screw a A 158 1260 Binary Pump User Manual Maintenance 10 Exchanging the Pump Seals When Seals leaking if indicated by the results of
135. tic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 1260 Binary Pump User Manual Introduction to the Binary Pump 1 Principle of Operation The binary pump is based on a two channel dual piston in series design which comprises all essential functions that a solvent delivery system has to fulfill Metering of solvent and delivery to the high pressure side are performed by two pump assemblies which can generate pressure up to 600 bar Each channel comprises a pump assembly including pump drive pump head active inlet valve with replaceable cartridge and outlet valve The two channels are fed into a low volume mixing chamber which is connected via a restriction capillary coil to a damping unit and a mixer A pressure sens
136. timizing Performance 5 When to Use the Low volume mixer The low volume mixer is designed for use with the Rapid Resolution LC system in low delay volume mode This configuration is typically used for 2 1 mm ID 1 8um particle size columns where emphasis is put on S N ratio The low volume mixer helps mixing gradients starting with a low concentration of organic solvents which can cause noise on the baseline The maximum benefit of the mixer is achieved using the mixer together with FW revisions A 06 06 or higher 1260 Binary Pump User Manual 91 5 Optimizing Performance When to Remove Damper and Mixer When to Remove Damper and Mixer The binary pump is equipped with a pressure pulsation damper and a static mixer The total delay volume of the pump is 600 800 uL depending on system pressure The mixer has a volume of 400 uL For applications that require lowest delay volume e g fast gradient methods or gradient applications with low flow rates damper and mixer can be bypassed Standard configuration Low delay volume configuration Figure 24 Flow Path Modifications of the Binary Pump 92 1260 Binary Pump User Manual Optimizing Performance 5 ten n M Convert the Binary Pump to Low Delay Volume Mode The binary pump is delivered in standard configuration damper and mixer connected This paragraph shows how to bypass damper and mixer and convert the pump to low delay volume mode Configurations where only damper or m
137. ting and diagnostic features gy Agilent Technologies 121 7 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Tests and Calibrations Compressibility Calibration Solvent compressibility is a function of solvent type and pressure In order to optimize flow accuracy and pressure ripple the compressibility of the solvent must be considered The binary pump firmware contains compressibility parameters for most commonly used solvents A compressibility calibration function is available to generate compressibility data for unlisted solvents see Binary Pump Solvent Calibration on page 136 The compressibility data are stored in an XML file and can be transferred to other G1312B pumps
138. ump User Manual Description Binary Pump Calibration capillary assembly LabAdvisor DVD 1200 Binary Pump SL Start up Kit Syringe adapter luer barb Syringe Plastic Solvent Cabinet Kit 1290 of Infinity Binary Pump complete Solvent cabinet 2 bottles for pumps without solvent selection valve Solvent bottle amber Solvent bottle transparent Column Agilent ZORBAX SB C18 2 1 x 50 mm1 8 pm Column SB C18 4 6x50 mm 1 8 pm 600 bar Column Eclipse XDB C18 4 6x50 mm 1 8 pm 600 bar Power cord Accessory Kit Accessory Kit 47 3 Installing the Pump Optimizing the Stack Configuration 48 1200 RRLC System in Standard Delay Volume Configuration This configuration is typically used when using 4 6 mm and 3 0 mm ID columns It is optimized for high flow rates and maximum sensitivity For a more detailed help on configuring your instrument refer to RRLC system configurator A 01 01 CD ROM p n 01200 60001 1260 Binary Pump User Manual Degasser Pump HiP ALS 0 17 mm seat Cooler for ALS TCC DAD SL Installing the Pump 3 Optimizing the Stack Configuration 400 mm x 0 17 mm 105 mm x 0 17 mm 105mm x 0 17 mm amp ZeroDeadVolume union 13 ul cell Figure 7 1200 RRLC system in standard delay volume configuration for 4 6 mm amp 3 0 mm id columns 1260 Binary Pump User Manual 49 3 Installing the Pump Optimizing the Stack Configuration 1200 RRLC in Medium Delay Volume Configuration
139. ump User Manual Parts and Materials for Maintenance 11 Hydraulic Path without Solvent Selection Valve ih Figure 38 Hydraulic Path with Solvent Selection Valve with Active Seal Wash 1260 Binary Pump User Manual 191 11 Parts and Materials for Maintenance Pump Head Assembly Without Seal Wash Item p n Description 1 5067 4695 Sapphire piston default 2 G1312 60062 Piston housing incl spring 3 G4220 63015 Support Ring without Seal Wash 4 G4220 24013 Backup Ring for Support Ring 5 G1312 87300 Absorber capillary 6 0905 1503 Piston seal PTFE carbon filled black pack of 2 default 7 G1312 25260 Pump housing 8 0515 0175 Mounting screw for manual purge valve holder M4 20 mm long 9 G1312 23200 Holder for manual purge valve 10 G1312 60061 Purge valve assembly 11 G1312 60067 Outlet valve complete 12 5042 1303 Screw lock 13 G1312 60066 Passive inlet valve 14 G1312 23201 Adapter 15 0515 2118 Screw M5 60 mm long 192 1260 Binary Pump User Manual Parts and Materials for Maintenance Pump Head Assembly Without Seal Wash 11 Figure 39 1260 Binary Pump User Manual Pump Head Assembly Without Seal Wash 193 11 Parts and Materials for Maintenance Pump Head Assembly with Seal Wash Option Item p n Description 1 5065 9953 Seal wash pump assembly 5042 8507 Seal wash pump cartridge 2 5067 4695 Sapphire piston default 3 G1312 60062 Piston housing incl spring 4 G4220 63010 Support Ring Seal W
140. ust be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1260 Binary Pump User Manual 223
141. utlet valve is not blocked the damper 3 Defective pump drive assembly Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative 1260 Binary Pump User Manual 113 6 Error Messages Motor Drive Power Motor Drive Power Pump channel A B Motor Drive Power Pump channel B The current drawn by the pump motor exceeded the maximum limit Blockages in the flow path are usually detected by the pressure sensor in the damper which result in the pump switching off when the upper pressure limit is exceeded If a blockage occurs before the damper the pressure increase cannot be detected by the pressure sensor and the module will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the module is switched off and the error message is generated Probable cause Suggested actions 1 Flow path blockage in front of the damper Ensure the capillaries and frits between the pump head and damper inlet are free from blockage 2 Blocked outlet valve Exchange the outlet valve 3 High friction partial mechanical blockage in Remove the pump head assembly Ensure there the pump drive assembly is no mechanical blockage of the pump head assembly or pump drive assembly 4 Defective pump drive assembly Please contact your Agilent service representative 5 Defective main board Please contact your Agilent service repres
142. uum Degasser 88 When to Use the Active Seal Wash Option 89 When to Use Alternative Seals 90 When to Use the Low volume mixer 91 When to Remove Damper and Mixer 92 How to Optimize the Compressibility Compensation Setting 95 Error Messages 99 What Are Error Messages 101 General Error Messages 102 Module Error Messages 108 Troubleshooting and Diagnostics 121 Overview of the Module s Indicators and Test Functions 122 Status Indicators 124 User Interfaces 126 Agilent Lab Advisor Software 127 Tests Functions and Calibration 129 Pressure Test Description 130 Pump Test 134 Binary Pump Solvent Calibration 136 Pump Elasticity Calibration 138 Diagnostic Signals 141 Analog Pressure Output 142 Diagnostic Signals in the ChemStation Software 143 Early Maintenance Feedback EMF 144 1260 Binary Pump User Manual Contents 10 Maintenance 147 Introduction to Maintenance and Repair 148 Warnings and Cautions 149 Cleaning the Module 150 Overview of Maintenance and Simple Repairs 151 Maintenance Procedures 152 Exchanging the Purge Valve Frit or the Purge Valve 154 Removing the Pump Head Assembly 156 Disassembling the pump head 158 Exchanging the Pump Seals 159 Seal Wear in Procedure 161 Exchanging the Pistons 162 Exchanging the Wash Seals 163 Reinstalling the Pump Head Assembly 165 Exchanging the Inlet Valve 167 Exchanging the Outlet Valve 169 Installation of the Solvent Selection Valve Upgrade Kit 171 Exchangin
143. witch settings provide configuration parameters for GPIB address serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN The following tables represent the configuration switch settings for the modules without on board LAN only 32 1260 Binary Pump User Manual Introduction to the Binary Pump 1 Table 6 8 bit Configuration Switch without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 rsvo sys rsvo rsvo Fc The LAN settings are done on the LAN Interface Card G1369A B Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 7 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down a
144. written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in
145. y purging the pump 1 Open the purge valve of your pump by turning it counterclockwise and set the flow rate to 5 mL min Flush the vacuum degasser and all tubes with at least 10 mL of solvent Repeat step 1 and 2 for the other channel s of the pump Set the required composition and flow rate for your application and close the purge valve 5 Pump for approximately 10 minutes before starting your application 66 1260 Binary Pump User Manual Installing the Pump 3 Changing Solvents When Parts required Preparations CAUTION When the solvent of a channel is to be replaced by another solvent that is not compatible solvents are immiscible or one solvent contains a buffer it is necessary to follow the procedure below to prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system Description 1 Purging solvent s see Table 15 on page 68 Remove the column and replace it by a ZDV fitting Prepare bottles with appropriate intermediate solvents see Table 15 on page 68 1 Ifthe channel is not filled with a buffer proceed to step 4 Place the solvent intake filter into a bottle of water Flush the channel at a flow rate suitable for the installed tubing typically 3 5 mL min for 10 min 4 Modify the flow path of your system as required for your application For delay volume optimization see the Rapid Resolution System manual Buffer salt of aqueous buffers may precipitate in
146. y Pump 1 The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table3 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 lt y 2 RX TX 3 x 3 TX DTR 4 4 4 DTR GND 5 y 5 GND DSR 6 6 DSR RTS 7 X 7 RTS CTS 8 f 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 4 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board 1260 Binary Pump User Manual 27 1 28 Introduction to the Binary Pump APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on
147. ysis bottles is below the given value When setting this parameter consider the size and shape of the solvent vessel and make sure the pump does not draw air when coming close to the limit Turn pump default turned off When ticked the pump will turn off before air is aspirated However the residual off solvent volume has been calculated for 1 L solvent bottles and may be too small for large bottles or other vessels 1260 Binary Pump User Manual 81 4 Using the Pump Early Maintenance Feedback EMF 82 Maintenance requires the exchange of components in the flow path which are subject to mechanical wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The binary pump provides a series of EMF counters for the left and right pump heads Each counter increments with pump use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The binary pump pr

Download Pdf Manuals

Related Search

Related Contents

Oster countertop oven Oven User Manual  CP5-Series User Manual  Andersen HD20V36WH Installation Guide      床下用換気扇  

Copyright © All rights reserved. Failed to retrieve file