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Analytical Sample Preparation

1. Columns and Plates ISOLUTE CA Chemical structure of C4 Silane covalently bonded to the surface of a silica No si O Si CH CH particle d Si OH Average particle size 50 um Pore diameter 60 Sorbent Type Non Polar Application Extraction from aqueous matrix using non polar interactions for acidic neutral and basic compounds Secondary silanol or ionic interactions can be used to enhance extract purity and method robustness for basic compounds ISOLUTE C4 SPE Columns Part Number Description Qty 390 0010 A ISOLUTE C4 100 mg 1 mL 100 390 0010 B ISOLUTE C4 100 mg 3 mL 50 390 0020 B ISOLUTE C4 200 mg 3 mL 50 390 0050 B ISOLUTE C4 500 mg 3 mL 50 390 0050 C ISOLUTE C4 500 mg 6 mL 30 390 0100 C ISOLUTE C4 1 g 6 mL 30 ISOLUTE 96 C4 Fixed Well Plates Part Number Description Qty 390 0025 1 ISOLUTE 96 C4 25 mg plate 1 ISOLUTE Array C4 Loose Wells Part Number Description Qty 390 0025 R ISOLUTE Array C4 25 mg 1 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE C4 TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents 27 28 SOLUTE Columns and Plates ISOLUTE C2 Chemica
2. Biotage 2015 ISOLUTE C8 EC Chemical structure of C8 Silane and trimethyl silyl group covalently bonded 1 to the surface of a silica O particle jSi O Si CH Average particle size 50 um Pore diameter 60 A Sorbent Type Non Polar Application Extraction from aqueous matrix using non polar interactions for acidic neutral and basic compounds ISOLUTE C8 EC SPE Columns Part Number Description Qty 291 0010 A ISOLUTE C8 EC 100 mg 1 mL 100 291 0010 B ISOLUTE C8 EC 100 mg 3 mL 50 291 0010 G ISOLUTE C8 EC 100 mg 10 mL 50 291 0020 B ISOLUTE C8 EC 200 mg 3 mL 50 291 0050 B ISOLUTE C8 EC 500 mg 3 mL 50 291 0050 C ISOLUTE C8 EC 500 mg 6 mL 30 291 0100 C ISOLUTE C8 EC 1 g 6 mL 30 291 0200 D ISOLUTE C8 EC 2 g 15 mL 20 291 0500 E ISOLUTE C8 EC 5 g 25 mL 20 ISOLUTE 96 C8 EC Fixed Well Plates Part Number Description Qty 291 0025 1 ISOLUTE 96 C8 EC 25 mg plate 1 291 0050 1 ISOLUTE 96 C8 EC 50 mg plate 1 291 0100 1 ISOLUTE 96 C8 EC 100 mg plate 1 ISOLUTE Array C8 EC Loose Wells Part Number Description Qty 291 0025 R ISOLUTE Array C8 EC 25 mg 1 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE C8 EC TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples SOLUTE
3. 420 0050 B 420 0050 C 420 0100 C 420 0100 P01 420 0100 T 430 0010 A 430 0010 B 430 0020 B 430 0050 B 430 0050 C 430 0050 H 430 0100 C 460 0002 A 460 0010 A 460 0010 B 460 0010 G 460 0020 B 460 0020 H 460 0050 B 460 0050 C 460 0050 H 460 0100 B 460 0100 C 460 0200 C 460 0200 D 460 0200 L 460 0500 E 460 1000 F 470 0002 A 470 0005 A 470 0010 A 470 0010 B 470 0010 G 470 0020 B 470 0050 B 470 0050 C 470 0050 H 470 0050 P01 470 0100 C 470 0100 P01 470 0200 C 470 0200 D 480 0010 A 480 0010 B 480 0020 B 480 0025 P01 480 0025 R 480 0050 B 480 0050 C 480 0100 C 480 0100 P01 480 0100 T 500 0002 A 62 62 62 62 62 29 29 29 29 29 29 29 29 29 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 33 34 500 0005 A 500 0005 G 500 0010 A 500 0010 B 500 0010 C 500 0010 G 500 0020 B 500 0020 H 500 0025 P01 500 0050 B 500 0050 C 500 0050 H 500 0050 P01 500 0050 R 500 0100 B 500 0100 C 500 0100 CG 500 0100 P01 500 0100 T 500 0200 D 503 0002 A 503 0005 A 503 0010 A 503 0010 B 503 0010 C 503 0020 B 503 0025 P01 503 0050 B 503 0050 C 503 0050 R 503 0100 C 503 0100 P01 503 0200 D 520 0002 A 520 0005 A 520 0010 A 520 0010 B 520 0020 B 520 0025 P01 520 0025 R 520 0050 B 520 0050 C 520 0050 P01 520 0050 R 520 0100 C 520 0100 P01 520 0200 D 530 0005 A 530 001
4. Processing Products 62 Biotage Extrahera SLE and SPE Automation System Biotage Extrahera Biotage Extrahera is a compact eight channel automation instrument designed for speed flexibility and with end user operation in mind The system has been designed to automatically process methods using well plate or column consumables Ideal for processing Supported Liquid Extraction SLE and Solid Phase Extraction SPE based methods The system benefits from a compact two level layout for solvent and sample pipette tips extraction consumables and samples The system also features an innovative lower level carousel based design and is capable of switching between plates or column in a matter of minutes The system processes a full standard SPE plate method in less than 30 minutes including sample pre treatment conditioning equilibration load wash and elution steps even when using volatile and low surface tension solvents Fully automated processing station Supports 96 amp 48 fixed well plates and 96 tabless 1 mL columns or 1 3 and 6 mL industry standard columns Features five automatically pump fed solvent reservoirs Built in controller with 12 touch screen graphical user interface Simple amp intuitive method creation Compact footprint Built in fume hood capabilities suitable for bench top usage Positive pressure processing for accurate control of flow Specified 50 to 1000
5. SOLUTE Myco solid phase extraction columns provide robust retlable sample preparation for multiple mycotosin classes HO from a wide range off uffs Ro ag a ting syt sample p tiga product g h optimized m note emi prepare dive ifcrog pies f alysis by LOMSM twe Analytes Aflatoxin 81 aflatoala 82 aflatoxin Gt aflatoxin G2 ergotryptine ergocornine ochratonin A fumonisin 81 zearalenone 1 2 mcot nis 2 mycotoxin Sample Preparation Procedure Column configuration ISOLLITE Myco 66 mg 3 mi column Tablews Part Number 150 0006 86 Sample pretreatment 1 Sample processing barley 90 g Store ground sample ina aled containe d int le 5 with 50 acetonitrile aq 20 mt e fer the extract to a 50 mi centrifuge tube and g cent oo 1 Dilution Take the supernatant 8 mil transfer to a new 30 ml centrifuge tube and dilute with water 32 mi Centrifuge diluted extract at 1000 g far a further 10 minutes 0 Biotage Qty 50 2015 Analytical Sample Preparation Catalog Biotage 2015 ISOLUTE Bulk Sorbents 44 SOLUTE Bulk Sorbents The same high quality sorbents used to manufacture ISOLUTE SPE columns and 96 well plates are also available in bulk The tightly controlled physical and chemical properties of these sorbents which provide reproducible performance in SPE procedures also ensure their suitability l q i BULK ISOLUF when used in applications that require loose material Ci Mean
6. 20 x 150 mm tubes P N C40709 C44283 Tube Rack 16 x 100 mm Prelude compatible C44880 Tube Rack for 15 mL Conical Centrifuge Tubes P N C44941 C47818 Tube Rack 15 mL Autotrace tubes with conical bottom suitable for tubes C47816 C47820 Tube Rack 10 mL conical tubes suitable for tubes C47811 C48928 Tube Rack 1 5 2 0 mL flip cap microcentrifuge tubes C48929 Tube Rack 1 5 2 0 mL screw cap microcentrifuge tubes C59546 Tube Rack GC vials C61345 Tube Rack ASE 40 60 mL IChem Vials For further information download the Evaporation brochure from www biotage com Literature part number PPS296 68 Index 101 Polystyrene divinylbenzene SPE columns 1 Point Support H 48 vvell plates 96 vvell plates 96 vvell accessories 96 vvell filtration plates A Accessories SPE and filtration Adaptors polyethylene for SPE columns Adaptors PTFE for SPE columns AFFINILUTE AL Alumina SPE columns Aminopropyl see NH2 Applications Aromatic sulfonic acid see SCX SCX 3 Array SPE wells and 96 well plates Automated Sample Processing Products Benzenesulfonic acid see SCX SCX 3 Bulk sorbents ISOLUTE Butyl see C4 C C2 C2 EC Ethyl 96 well SPE plates C4 Butyl 96 well SPE plates SPE columns C8 C8 EC Octyl 96 well SPE plates Bulk sorbent SPE columns C18 C18 EC n Octadecyl 96 well SPE plates Bulk sorbent SPE columns Caps SPE column Carboxypropyl acid see CBA CBA Carboxypropyl
7. 22 604 0025 R 604 0030 PX01 610 0005 B 610 0005 BG 610 0010 B 610 0010 H 610 0020 B 610 0020 C 610 0050 C 611 0005 B 611 0005 BG 611 0010 B 611 0010 BG 611 0010 H 611 0020 B 611 0020 C 611 0050 C 611 0050 CG 612 0005 B 612 0010 B 612 0010 H 612 0020 C 612 0050 C 612 0050 CG 613 0005 B 613 0010 B 613 0010 BG 613 0010 H 613 0020 C 613 0050 C 613 0050 CG 614 0005 B 614 0010 B 614 0010 H 614 0020 C 614 0050 C 650 0010 PX01 650 0030 PX01 712 0010 A 712 0020 B 712 0050 B 712 0050 BG 712 0050 C 712 0050 H 712 0050 L 712 0100 B 712 0100 C 712 0200 D 712 0500 E 712 2000 F 713 0010 A 713 0020 B 713 0050 B 713 0050 C 713 0100 B 713 0100 C 713 0200 D 713 0500 E 714 0010 A 714 0020 B 714 0050 B 714 0050 C 714 0100 B 714 0100 C 22 22 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 21 21 21 21 21 21 21 21 21 21 21 21 21 22 22 22 22 22 22 22 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 714 0200 C 714 0200 D 714 0500 E 714 1000 F 715 0010 A 715 0020 B 715 0050 B 715 0050 C 715 0100 B 715 0100 C 715 0200 D 715 0500 E 715 1000 F 752 0100 C 753 0100 CD 753 0300 FD 800 0040 BM 800 0100 CM 800 0220 DM 800 0350 EM 800 0700 FM 800 1300 FM 802 0250 M 820 0055 B 820 0055 B 500 820 0055 BG 820 0055 BG 500 820 0140 C 820 0140 C 1000 820 0140 CG 820 0140 CG 1000 820 0200 P01 820 0200 T 820 0290 D 820 0290
8. Advanced Polymeric Sorbents EVOLUTE WAX en Or Del R R e TN TN Low pH RNH3 High pH Chemical structure of EVOLUTE WAX Chemical Description Primary secondary amine modified polystyrene divinylbenzene incorporating non ionizable hydroxyl groups Average particle size Pore diameter 40A Sorbent Type 30 um 50 um Mixed mode non polar weak cation exchange pKa 5 Exchange capacity 0 3 mmol g 0 7 mmol g Application EVOLUTE WAX can be used to extract strongly acidic analytes e g sulfonic acids from biological fluids and other aqueous matrices Performance is not affected by drying the sorbent EVOLUTE WAX 30 um SPE Columns Part Number Description Qty 604 0002 A EVOLUTE WAX 25 mg 1 mL 100 EVOLUTE WAX 50 um SPE Columns 614 0005 B EVOLUTE WAX 50 mg 3 mL 50 614 0010 B EVOLUTE WAX 100 mg 3 mL 50 614 0010 H EVOLUTE WAX 100 mg 10 mL XL 50 614 0020 C EVOLUTE WAX 200 mg 6 mL 30 614 0050 C EVOLUTE WAX 500 mg 6 mL 30 EVOLUTE EXPRESS WAX 30 um Fixed Well Plates 604 0010 PX01 EVOLUTE EXPRESS WAX 10 mg 1 Fixed Well Plate 604 0030 PX01 EVOLUTE EXPRESS WAX 30 mg 1 Fixed Well Plate EVOLUTE WAX 30 um Array Loose Wells 604 0025 R EVOLUTE Array WAX 25 mg imL Wells 100 EVOLUTE EXPRESS Sorbent Selection Plate The EVOLUTE EXPRESS Sorbent Selection plate contains the four mixed mode EVOLUTE sorbents CX WCX AX and WAX on one plate EVOLUTE WAX gt EVOLUTE WCX Method 1 EVOLUT
9. C18 500 mg 6 mL Glass 30 Depth filter 220 0100 B ISOLUTE C18 1 9 3 mL 50 221 0050 H ISOLUTE C18 EC 500 mg 10 mL 50 220 0100 C ISOLUTE C18 1 g 6 mL 30 221 0100 B ISOLUTE C18 EC 1 g 3 mL 50 220 0100 CG ISOLUTE C18 1 g 6 mL Tabless 30 221 0100 C ISOLUTE C18 EC 1 g 6 mL 30 220 0200 C ISOLUTE C18 2 g 6 mL 30 221 0200 C ISOLUTE C18 EC 2 g 6 mL 30 220 0200 D ISOLUTE C18 2 g 15 mL 20 221 0200 D ISOLUTE C18 EC 2 g 15 mL 20 220 0500 E ISOLUTE C18 59 25 mL 20 221 0500 E ISOLUTE C18 EC 5 g 25 mL 20 220 1000 F ISOLUTE C18 10 g 70 mL 16 221 1000 F ISOLUTE C18 EC 10 g 70 mL 16 ISOLUTE 96 C18 Fixed Well Plates ISOLUTE 96 C18 EC Fixed Well Plates Part Number Description Qty Part Number Description Qty 220 0025 1 ISOLUTE 96 C18 25 mg plate 1 221 0025 P01 ISOLUTE 96 18 25 mg plate 1 220 0050 1 ISOLUTE 96 C18 50 mg plate 1 221 0050 1 ISOLUTE 96 C18 EC 50 mg plate 1 220 0100 1 ISOLUTE 96 C18 100 mg plate 1 221 0100 1 ISOLUTE 96 C18 EC 100 mg plate 1 ISOLUTE Array C18 Loose VVells ISOLUTE Array C18 EC Loose Wells Part Number Description Qty Part Number Description Qty 220 0025 R ISOLUTE Array C18 25 mg 1 mL wells 100 221 0025 R ISOLUTE Array C18 EC 25 mg 1 mL wells 100 220 0050 R ISOLUTE Array C18 50 mg 1 mL wells 100 221 0100 T ISOLUTE Array C18 EC 100 mg 2 mL wells 100 220 0100 T ISOLUTE Array C18 100 mg 2 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order Pre assembled ISOLUTE Array plates ar
10. D 1000 820 0400 P01 820 0400 T 820 0690 E 820 1000 Q01 820 1420 F 902 0002 A 902 0002 AG 902 0005 A 902 0010 A 902 0010 B 902 0013 A 902 0013 B 902 0013 BG 902 0015 C 902 0013 CG 902 0013 H 902 0020 B 902 0020 H 902 0025 P01 902 0025 R 902 0030 B 902 0030 C 902 0030 CG 902 0030 H 902 0050 C 902 0050 P01 902 0050 T 902 0100 P01 902 0100 38 38 38 38 38 38 38 38 38 38 38 38 38 39 40 40 38 38 38 38 38 38 53 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 2015 Analytical Sample Preparation Catalog Biotage 2015 903 0005 A 903 0010 A 903 0020 B 903 0020 C 903 0020 H 903 0025 P01 903 0025 R 903 0100 P01 904 0010 A 904 0030 B 904 0030 C 904 0050 B 904 0100 C 905 0002 A 905 0005 A 905 0010 A 905 0025 01 905 0025 905 0100 P01 905 0100 T 906 0002 A 906 0005 A 906 0010 A 906 0010 G 906 0013 H 906 0025 P01 906 0025 R 906 0100 P01 915 0001 A 915 0002 A 915 0002 G 915 0005 A 915 0005 B 915 0005 G 915 0010 A 915 0010 B 915 0010 BG 915 0010 C 915 0010 H 915 0010 P01 915 0020 B 915 0020 C 915 0020 CD 915 0020 CG 915 0025 P01 915 0025 R 915 0050 B 915 0050 BG 915 0050 C 915 0050 D 915 0050 L 915 0100 C 915 0100 E 918 0050 P01 9220 0100 9221 0100 924 0050 C 924 0100 C 927 0075 B 927 0150 C 927 0150 CD 9
11. Particl Size Micron 4 7 erage Pore Size Average Pora lili QUANTITY Non Polar Sorbents nA b l crue Part Number Description Qty oral Internations So 2 m 9220 0100 ISOLUTE C18 bulk 100 g Ma LA 9221 0100 ISOLUTE C18 EC bulk 100 g Prone 6 9290 0100 ISOLUTE C8 bulk 100 g 9291 0100 ISOLUTE C8 EC bulk 100 g 9320 0100 ISOLUTE C2 bulk 100 g 9321 0100 ISOLUTE C2 EC bulk 100 g EC endcapped a chemical process to reduce the concentration of silica surface silanol groups that provide polar and weak cation exchange secondary interactions For more details see the QuickStart Guide to SPE Literature part number QSG_2013 V1 Polar Sorbents Part Number Description Qty 9460 0100 ISOLUTE SI bulk 100 g 9470 0100 ISOLUTE NH2 bulk 100 g 9480 0100 ISOLUTE PSA bulk 100 g 9712 0100 ISOLUTE FL bulk 100g Ion Exchange Sorbents Part Number Description Qty 9470 0100 ISOLUTE NH2 bulk 100 g 9480 0100 ISOLUTE PSA bulk 100 g 9500 0100 ISOLUTE SAX bulk 100 g 9503 0100 ISOLUTE PE AX bulk 100 g 9520 0100 ISOLUTE CBA bulk 100 g 9532 0100 ISOLUTE SCX 2 bulk 100 g 9533 0100 ISOLUTE SCX 3 bulk 100 g ISOLUTE sorbents are available in additional quantities to those detailed in the above table ISOLUTE HM N a modified form of diatomaceous earth is also available in bulk The material is used for applications requiring an inert support as part of the sample preparation process e g SFE For details of pr
12. Plates 17 Protein precipitation plates 45 Propylsulfonic acid see SCX 2 PRS PSA Primary secondary amine 96 well SPE plates 33 Bulk sorbent 44 SPE columns 33 PTFE adaptors 52 PTFE needles VacMaster 57 PTFE stopcocks VacMaster 57 PTFE stopcock needles VacMaster 57 PTFE stopcocks for universal use 57 Q Quality issues 7 Quaternary amine see PE AX and SAX QuEChERS 47 QuickStart Guide to SPE 4 R Rack Extrahera 62 Rack Gravity 60 Rack TurboVap 67 RapidTrace 63 Rack for VacMaster 57 Reservoirs 55 s Sample Preparation Format Options 10 11 SAX Quaternary amine 96 well SPE plates 34 SPE columns 34 SAX PSA SPE columns 39 SCX Benzenesulfonic acid 96 well SPE plates 35 SPE columns 35 SCX 2 Propylsulfonic acid 96 well SPE plates 36 Bulk sorbent 44 SPE columns 36 SCX 3 Ethylbenzene sulfonic acid 96 well SPE plates 36 SPE columns 36 SI Silica Bulk sorbent 44 SPE columns 37 SLE plates and columns 14 Sodium sulfate drying cartridge 53 Solvent evaporation 65 67 Solvent trap 59 Sorbents Bulk ISOLUTE 43 SPE Dry 96 sample concentrator systems 66 Stainless steel needles VacMaster 57 Stopcock needles PTFE 57 Stopcocks PTFE for universal use 57 Strong anion exchange see SAX PE AX Strong cation exchange see SCX 2 3 Supported Liquid Extraction SLE columns and plates 13 also see HM N columns and SLE plates Styrene divinyl benzene SDVB s
13. SLE and filtration applications MEME VacMaster 10 Sample Processing Manifold Part Number Description 121 1010 121 1012 121 1016 VacMaster 10 Sample Processing Manifold with 10 mm rack VacMaster 10 Sample Processing Manifold with 12 mm rack VacMaster 10 Sample Processing Manifold with 16 mm rack VacMaster 10 Replacement Parts Part Number Description Qty 121 1039 Replacement tank vvith fittings 1 121 1045 Replacement lid vvith fittings 1 121 1030 Silicone Lid Gasket 1 121 1132 Replacement rack 10 mm 1 121 1133 Replacement rack 12 mm 1 121 1134 Replacement rack 16 mm 1 VacMaster 20 Sample Processing Manifold Part Number Description 121 2010 Sample Processing Manifold with 10 mm rack 121 2012 Sample Processing Manifold with 12 mm rack 121 2016 Sample Processing Manifold with 16 mm rack VacMaster 20 Replacement Parts Part Number Description Qty 121 2068 Replacement tank vvith fittings 1 121 2075 Replacement lid vvith fittings 1 121 2059 Silicone Lid Gasket 1 121 2161 Replacement rack 10 mm 1 121 2162 Replacement rack 12 mm 1 121 2163 Replacement rack 16 mm 1 VacMaster Accessories Part Number Description Qty 121 0010 PTFE T valve for connecting 2 tanks 1 Biotage VacMaster 10 amp 20 Sample Processing Manifolds Part Number Description 121 0009 121 0001 121 0002 121 0003 121 0004 121 0005 121 0009 5 Universal PTFE stopcock PTFE stopcock needl
14. SOLUTES PERS EYOLULE EXPRESS SOLLTE Sikes Your Complete Partner for Effective Chemistry Biotage is a worldwide supplier of instruments and accessories designed to facilitate the work of laboratory chemists With our deep knowledge of the industry academic contacts and in house R amp D teams we can deliver the best solutions to your challenges We take great pride in our flexibility and ability to meet our customer s individual needs With strong foundations in both analytical and organic chemistry we can offer the widest range of solutions available on the market Biotage Extrahera EUROPE NORTH amp LATIN AMERICA JAPAN CHINA Main Office 46 18 565900 Main Office 1 704 654 4900 Tel 81 3 5627 3123 Tel 86 21 2898 6655 Toll Free 800 18 565710 Toll Free 1 800 446 4752 Fax 81 3 5627 3121 Fax 86 21 2898 6153 Fax 46 18 591922 Fax 1 704 654 4917 jp_order biotage com cn_order biotage com Order Tel 46 18 565710 Order Tel 1 704 654 4900 jp 1 pointsupport biotage com cn 1 pointsupport biotage com Order Fax 46 18 565705 Order Fax 1 434 296 8217 order biotage com ordermailbox biotage com To locate a distributor Support Tel 46 18 56 59 11 Support Tel 1 800 446 4752 please visit our website at Support Fax 46 18 56 57 11 Outside US 1 704 654 4900 www biotage com eu 1 pointsupport biotage com us 1 pointsupport biotage com Part Number PPS354 V 1 2015 Biotage All rights reserved No
15. Support Documents for ISOLUTE PH TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents SOLUTE Columns and Plates ISOLUTE CN Chemical structure of cyanopropyl silane covalently bonded to the surface of a silica particle Si O i CH CN Si OH l Average particle size 50 um Pore diameter 60 Sorbent Type Non Polar Application Extraction from aqueous matrix using both non polar interactions for acidic neutral and basic compounds and secondary silanol or ionic interactions for basic compounds only Can also be used in polar SPE mode ISOLUTE CN SPE Columns Part Number Description Qty 420 0010 A ISOLUTE CN 100 mg i mL 100 420 0010 B ISOLUTE CN 100 mg 3 mL 50 420 0020 B ISOLUTE CN 200 mg 3 mL 50 420 0050 B ISOLUTE CN 500 mg 3 mL 50 420 0050 C ISOLUTE CN 500 mg 6 mL 30 420 0100 C ISOLUTE 1 g 6 mL 30 ISOLUTE 96 CN Fixed Well Plates Part Number Description Qty 420 0025 P01 ISOLUTE 96 CN 25 mg plate 1 420 0100 P01 ISOLUTE 96 100 mg plate 1 ISOLUTE Array CN Loose Wells Part Number Description Qty 420 0100 T ISOLUTE Array CN 100 mg 2 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documen
16. User Guide Supported Liquid Extraction SLE is a quick easy and cost effective re sample preparation technique for agnen analytical chemistry This guide describes how to develop supported liquid extraction methods using ISOLUTE SLE products along with hints and tips for optimizing 8 Biotage performance and extending the range of analytes that can be extracted Literature part number U1304 V 2 EVOLUTE User Guide The EVOLUTE User Guide contains a wealth of technical information EVOLUTE User Guide and practical tips to make method development using EVOLUTE SPE products fast and simple The guide describes generic methodologies and method optimization strategies for use with all the EVOLUTE sorbents and introduces the streamlined Load Wash Elute procedure for EVOLUTE EXPRESS products Literature part number EVO UG_2012 rev 1 E Biotage SPE QuickStart Guide Use our QuickStart Guide for an QuickStart Guide to SPE introduction to SPE Literature part number QSG_2013_V 1 To download a copy of any of the above guides please visit www biotage com 6 Biotage 2015 Analytical Sample Preparation Catalog Biotage 2015 Contact Biotage 1 Point Support Contact Biotage 1 Point Support VVVVVV biotage com The Biotage vveb site offers our customers easy access to current information on new products applications and events Europe Service and Support Telep
17. and method robustness for basic compounds ISOLUTE C8 SPE Columns Part Number Description Qty 290 0002 A ISOLUTE C8 25 mg 1 mL 100 290 0005 A ISOLUTE C8 50 mg 1 mL 100 290 0010 A ISOLUTE C8 100 mg 1 mL 100 290 0010 B ISOLUTE C8 100 mg 3 mL 50 290 0010 C ISOLUTE C8 100 mg 6 mL 30 290 0020 B ISOLUTE C8 200 mg 3 mL 50 290 0020 H ISOLUTE C8 200 mg 10 mL 50 290 0050 B ISOLUTE C8 500 mg 3 mL 50 290 0050 C ISOLUTE C8 500 mg 6 mL 30 290 0100 C ISOLUTE C8 1 g 6 mL 30 ISOLUTE 96 C8 Fixed Well Plates Part Number Description Qty 290 0025 1 ISOLUTE 96 C8 25 mg plate 1 290 0050 1 ISOLUTE 96 C8 50 mg plate 1 290 0100 1 ISOLUTE 96 C8 100 mg plate 1 ISOLUTE Array C8 Loose Wells Part Number Description Qty 290 0025 R ISOLUTE Array C8 25 mg 1 mL wells 100 290 0050 R ISOLUTE Array C8 50 mg 1 mL wells 100 290 0100 T ISOLUTE Array C8 100 mg 2 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE C8 TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents TN126 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Biological Fluid Samples 2015 Analytical Sample Preparation Catalog
18. available contact Biotage for details 2015 Analytical Sample Preparation Catalog Biotage 2015 ISOLUTE SI Chemical structure of b silanol groups on the SI OH surface of a silica particle SI OH Average particle size 50 um Pore diameter 60 Sorbent Type Polar Nominal moisture content 7 Application Extraction of polar compounds from a non polar sample matrix using hydrogen bonding retention mechanism ISOLUTE SI SPE Columns Part Number Description Qty 460 0002 A ISOLUTE SI 25 mg 1 mL 100 460 0010 A ISOLUTE SI 100 mg 1 mL 100 460 0010 B ISOLUTE SI 100 mg 3 mL 50 460 0010 G ISOLUTE SI 100 mg 10 mL 50 460 0020 B ISOLUTE SI 200 mg 3 mL 50 460 0020 H ISOLUTE SI 200 mg 10 mL 50 460 0050 B ISOLUTE SI 500 mg 3 mL 50 460 0050 C ISOLUTE SI 500 mg 6 mL 30 460 0050 H ISOLUTE SI 500 mg 10 mL 50 460 0100 B ISOLUTE SI 1 g 3 mL 50 460 0100 C ISOLUTE SI 1 g 6 mL 30 460 0200 C ISOLUTE SI 2 g 6 mL 30 460 0200 L ISOLUTE SI 2 g 6 mL Glass 30 460 0200 D ISOLUTE SI 2 9 15 mL 20 460 0500 E ISOLUTE SI 5 g 25 mL 20 460 1000 F ISOLUTE SI 10 g 70 mL 16 Support Documents for ISOLUTE SI TN102 Method Development in Solid Phase Extraction using Polar ISOLUTE SPE Columns for the Extraction of Non aqueous Samples SOLUTE Columns and Plates ISOLUTE DIOL Chemical structure of DIOL Silane covalently bonded NE Si 0 Si CH OCH CH CH to the surface of a silica particle OH OH Si OH Aver
19. exchange pKa 10 1 and 10 9 or polar Exchange capacity 0 4 meq g Application ISOLUTE PSA can be used to extract strong acids and polyacidic compound from aqueous sample matrix Analyte elution can be achieved by neutralizing the charge on the sorbent Sorbent will complex with certain metal ions Supplied as free base Alternatively ISOLUTE PSA can be used for extraction of polar compounds from a non polar matrix using a hydrogen bonding retention mechanism Less retentive than silica when used in this mode ISOLUTE PSA SPE Columns Part Number Description Qty 480 0010 A ISOLUTE PSA 100 mg 1 mL 100 480 0010 B ISOLUTE PSA 100 mg 3 mL 50 480 0020 B ISOLUTE PSA 200 mg 3 mL 50 480 0050 B ISOLUTE PSA 500 mg 3 mL 50 480 0050 C ISOLUTE PSA 500 mg 6 mL 30 480 0100 C ISOLUTE PSA 1 g 6 mL 30 ISOLUTE 96 PSA Fixed Well Plates Part Number Description Qty 480 0025 1 TSOLUTE 96 PSA 25 mg plate 1 480 0100 P01 ISOLUTE 96 PSA 100 mg plate 1 ISOLUTE Array PSA Loose Wells Part Number Description Qty 480 0025 R ISOLUTE Array PSA 25 mg 1 mL wells 100 480 0100 T ISOLUTE Array PSA 100 mg 2 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE PSA TN102 Method Development in Solid Phase Extraction using Polar ISOLUTE SPE Columns for the Extraction of Non aqueous Samples TN105 Method Development in Solid Phase Extract
20. lt 0 1 End Capping Test PAS s Exchange Capacity N A Particle Size Analysis Special Recovery Test N A Elemental Analysis C18 EC BATCH No 221 0 02 Packed Column and Component Test Column Flovv Resistance PASS Column Purity Test GC PASS Frit Purity Test GC PASS Material Weight Check PASS 2 1 1 10 100 Average Particle Diameter um N A Not Applicable tage of Particles 10um Percentage of Particles gt 90 Biotage welcomes feedback on all Quality issues Percentage of Particles 30 90 This ISOLUTE product has passed jhe Biotage s rigorous QC tests Gr Quality Control Manager Europe North America Japan Service and Support Service and Support Service and Support Telephone 46 18 56 59 11 Telephone 1 800 446 4752 Telephone 81 3 5627 3124 E mail EU 1 PointSupport biotage com press 3 at the auto attendant E mail JP 1 PointSupport biotage com e mail US 1 PointSupport biotage com Europe and ROW if your country is not listed here contact your local distributor Please visit our website at www biotage com for your local distributor contact details 2010 Biotage All rights reserved ISOLUTE is a registered trademark of Biotage AB Reproducible optimized chemistry for ISOLUTE and EVOLUTE sorbents e Minimize method development time e Eliminate need for time consuming method changes when different batches of sorbent are used High and reproducible capacities of ISOLUTE ion exchange
21. non ionizable hydroxyl groups Average particle size Pore diameter 40A Sorbent Type 30 um 50 um Mixed mode non polar strong cation exchange Exchange capacity 0 5 mmol g Application EVOLUTE CX can be used to extract basic analytes from biological fluids and other aqueous matrices Performance is not affected by drying the sorbent EVOLUTE CX 30 pm SPE Columns Part Number Description Qty 601 0001 A EVOLUTE CX 10 mg 1 mL 100 601 0001 AG EVOLUTE CX 10 mg 1 mL Tabless 100 601 0002 A EVOLUTE CX 25 mg 1 mL 100 601 0002 AG EVOLUTE CX 25 mg 1 mL Tabless 100 EVOLUTE CX 50 um SPE Columns 611 0005 B EVOLUTE CX 50 mg 3 mL 50 611 0005 BG EVOLUTE CX 50 mg 3 mL Tabless 50 611 0010 B EVOLUTE CX 100 mg 3 mL 50 611 0010 BG EVOLUTE CX 100 mg 3 mL Tabless 50 611 0010 H EVOLUTE CX 100 mg 10 mL XL 50 611 0020 B EVOLUTE CX 200 mg 3 mL 50 611 0020 C EVOLUTE CX 200 mg 6 mL 30 611 0050 C EVOLUTE CX 500 mg 6 mL 30 611 0050 CG EVOLUTE CX 500 mg 6 mL Tabless 30 EVOLUTE EXPRESS CX 30 um Fixed Well Plates 601 0010 PX01 EVOLUTE EXPRESS CX 1 10 mg Fixed Vvell Plate 601 0030 PX01 EVOLUTE EXPRESS CX 1 30 mg Fixed Well Plate EVOLUTE CX 30 um Pre Assembled 96 Well Plates 601 0010 RP EVOLUTE Array CX 10 mg 1 mL Plate 1 601 0025 RP EVOLUTE Array CX 25 mg 1 mL Plate 1 EVOLUTE CX 30 um Array Loose Wells 601 0010 R EVOLUTE Array CX 10 mg 1 mL Wells 100 601 0025 R EVOLUTE Array CX 25 mg 1 mL Wells 100 EVOLUTE EXPRESS products can be us
22. sorbents e Reproducible high recoveries in ion exchange SPE without using columns with excessively large sorbent mass e Saves time money and increases analyte concentration in final extract High purity sorbents frits columns and 96 Well SPE plates e The purity of all the components used to manufacture ISOLUTE and EVOLUTE SPE products are monitored to ensure compatibility with the most demanding applications 2015 Analytical Sample Preparation Catalog Biotage 2015 Sample Preparation Format Options 10 Sample Preparation Format Options Actual Size Columns C amp L mL 3 mL 6 mL 15mL 25mL 70 mL U D U TT r All the SPE column and u u reservoir size options are T illustrated here as actual size 2 mL dravvings The last character of the SOLUTE or EVOLUTE SPE column or empty reservoir part number identifies the column R reservoir size For example the Tiit 100 mg C18 ISOEUME column P N 220 0010 A uses column A whereas the 100 mg C18 ISOLUTE XL SPE column P N 220 0010 G contains the same sorbent and sorbent mass packed in column G The nominal volume listed for each reservoir is for the reservoir without sorbent Tabless columns 1 3 and 6 mL for use on Biotage PRESSURE manifolds are also available ISOLUTE Array 2015 Analytical Sample Preparation Catalog Biotage 2015 Sample Preparation Format Options Actual Size
23. such media in accordance with Biotage s standard operating procedures Should any Biotage consumable fail to meet the limited warranty above after being tested in accordance with the applicable Biotage standard operating procedures Biotage will provide at its sole option either a replacement cartridge or reaction vial If a cartridge it will be packed with the original media or packed with new media at no cost to the Purchaser If such failed cartridge is packed with media provided by the Purchaser Biotage will make a reasonable effort to re pack the original media or pack a replacement cartridge with new media provided by the Purchaser Returns of any Consumable must be authorized in advance Please contact Biotage for a Return Authorization RA number and shipping instructions All claims must be made within thirty 30 days of shipment from Biotage or after the initial test of the cartridge at the Customer s facility whichever is first BIOTAGE MAKES NO OTHER WARRANTY EXPRESSED OR IMPLIED INCLUDING MERCHANTABILITY OR SUITABILITY FOR ANY PURPOSE BIOTAGE WILL NOT BE LIABLE IN ANY EVENT FOR SPECIAL INDIRECT OR CONSEQUENTIAL DAMAGES WHETHER ARISING IN TORT UNDER ANY WARRANTY OR OTHERWISE 2015 Analytical Sample Preparation Catalog Biotage 2015 Notes 75 76 Notes 2015 Analytical Sample Preparation Catalog Biotage 2015 a R ep D x ean l Piste 9 my Bi Inn 0 20 in a Im otage 46
24. uL liquid handling performance Aspirate dispense mixing Ultrasonic sensing Three elution positions for fraction collection and multiple elution Biotage Extrahera Brochure For further information download the Extrahera brochure from www biotage com Literature part number PPS353 Biotage Extrahera SLE and SPE Automation System LL m eee 1 6 Biotage Part Number 414001 414007 414008 414141 413640SP 414174SP 414169SP 414253SP 414254SP 414255SP 414256SP 414257SP 414045SP 414203SP 414201SP Description Biotage Extrahera Configuration Kit 96 Positions Configuration Kit 24 Positions Biotage Disposable Tips 1000 uL Clear Column Rack 24 x 6 mL tabless Column Rack 24 x 3 mL Column Rack 24 x 1 mL Column Rack 96 x 1 mL tabless Sample Rack 16 x 100 mm 24 Positions Sample Rack 13 x 100 mm 24 Positions Sample Rack 12 x 75 mm 24 Positions Collection Rack 18 mm 24 Positions Solvent Reservoir 25 mL Flow Through Plate 24 Flow Through Plate 96 Eatrahera Hmm Blotage Extraher urn Qty 1 1 1 10 x Pk 96 2015 Analytical Sample Preparation Catalog Biotage 2015 RapidTrace Automated High Throughput Solid Phase Extraction Reagent mixing chamber allows automated online reagent blending in the method Closed flu
25. 0 A 530 0010 B 530 0020 B 530 0025 P01 530 0050 B 530 0050 C 530 0050 H 530 0050 P01 530 0050 R 530 0100 B 530 0100 C 530 0100 P01 530 0100 T 530 0200 D 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 34 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 35 71 72 532 0002 A 532 0005 A 532 0010 A 532 0010 B 532 0020 B 532 0025 P01 532 0025 R 532 0050 B 532 0050 C 532 0050 H 532 0050 P01 532 0100 B 532 0100 C 532 0100 CG 532 0100 P01 532 0200 D 533 0002 A 533 0005 A 533 0010 A 533 0010 B 533 0020 B 533 0025 P01 533 0025 R 533 0050 B 533 0050 C 533 0050 H 533 0050 P01 533 0100 B 533 0100 C 533 0100 P01 533 0100 T 533 0200 D 600 0001 A 600 0002 A 600 0002 AG 600 0002 B 600 0002 H 600 0010 PX01 600 0010 R 600 0010 RP 600 0025 R 600 0025 RP 600 0030 PX01 601 0001 A 601 0001 AG 601 0002 A 601 0002 AG 601 0010 PX01 601 0010 R 601 0010 RP 601 0025 R 601 0025 RP 601 0030 PX01 602 0002 A 602 0010 PX01 602 0025 R 602 0030 PX01 603 0002 A 603 0002 AG 603 0010 PX01 603 0025 R 603 0030 PX01 604 0002 A 604 0010 PX01 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 36 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 21 21 21 21 21 21 21 21 21 22
26. 0 D ISOLUTE PE AX 2 g 15 mL 20 ISOLUTE 96 PE AX Fixed Well Plates Part Number Description Qty 503 0025 1 ISOLUTE 96 25 mg plate 1 503 0100 P01 ISOLUTE 96 PE AX 100 mg plate 1 ISOLUTE Array PE AX Loose Wells Part Number Description Qty 503 0050 R ISOLUTE Array PE AX 50 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE TN103 Method Development in Solid Phase Extraction using ISOLUTE Part Number Description Qty 500 0002 A ISOLUTE SAX 25 mg 1 mL 100 500 0005 A ISOLUTE SAX 50 mg 1 mL 100 500 0005 G ISOLUTE SAX 50 mg 10 mL 50 500 0010 A ISOLUTE SAX 100 mg 1 mL 100 500 0010 B ISOLUTE SAX 100 mg 3 mL 50 500 0010 C ISOLUTE SAX 100 mg 6 mL 30 500 0010 G ISOLUTE SAX 100 mg 10 mL 50 500 0020 B ISOLUTE SAX 200 mg 3 mL 50 500 0020 H ISOLUTE SAX 200 mg 10 mL 50 500 0050 B ISOLUTE SAX 500 mg 3 mL 50 500 0050 C ISOLUTE SAX 500 mg 6 mL 30 500 0050 H ISOLUTE SAX 500 mg 10 mL 50 500 0100 B ISOLUTE SAX 1 g 3 mL 50 500 0100 C ISOLUTE SAX 1 g 6 mL 30 500 0100 CG ISOLUTE SAX 1 g 6 mL Tabless 30 500 0200 D ISOLUTE SAX 2 g 15 mL 20 ISOLUTE 96 SAX Fixed Well Plates Part Number Description Qty 500 0025 1 ISOLUTE 96 SAX 25 mg plate 1 500 0050 1 ISOLUTE 96 SAX 50 mg plate 1 500 0100 1 ISOLUTE 96 SAX 100 mg plate 1 ISOLUTE Array SAX Loose Wells Part Number Description Qty 500 0050 R ISOLUTE A
27. 0 g 70 mL 16 714 1000 F 715 1000 F 2015 Analytical Sample Preparation Catalog Biotage 2015 ISOLUTE EPH SPE Columns Fractionate extractable petroleum hydrocarbons in soil extracts prior to GC analysis ISOLUTE EPH columns are optimized to ensure no breakthrough of lower MW aromatic hydrocarbons PAHs in the aliphatic fraction Application notes with methodology optimized for automated SPE processing are available Download application note AN703 ASPEC or AN704 RapidTrace for automated methods Part Number Description Qty 928 0145 B ISOLUTE EPH 1 45 g 3 mL 50 Columns for automated method 928 0500 E ISOLUTE EPH 5 g 25 mL 20 Columns for manual method ISOLUTE Layered SPE Columns Simultaneously extract analytes with a broad range of polarity characteristics from aqueous samples Part Number Description Qty 933 0050 B ISOLUTE C2 C18 EC 500 mg 3 mL 50 933 0100 C ISOLUTE C2 C18 EC 1 g 6 mL 30 934 0040 C ISOLUTE C8 ENV 400 mg 6 mL 30 935 0040 C ISOLUTE C18 ENV 400 mg 6 mL 30 941 0020 B ISOLUTE C2 ENV 200 mg 3 mL 50 SOLUTE Columns and Plates ISOLUTE PAH SPE Columns Extract PAHs from water samples containing polar interferences such as humic acids The layered column removes humic acids from the final extract so that they cannot interfere in the final analysis Download application note IST1025A for more information Part Number Description Qty 927 0075 B ISOLUTE PAH 750 mg 3 mL 50 927 0150
28. 01 0121 A 52 121 2195 59 221 0050 C 25 1201 0122 B 52 121 5202 15 18 54 56 58 221 0050 CD 25 1201 0123 C 52 121 5203 15 18 54 56 58 221 0050 H 25 1201 0125 H 52 121 5204 54 221 0050 P01 25 1201 0126 D 52 121 5205 54 221 0100 B 25 1201 0127 E 52 121 5210 15 221 0100 C 25 1201 0128 F 52 121 9600 58 221 0100 P01 25 120 1021 A 53 121 9601 58 221 0100 T 25 120 1022 B 53 121 9602 58 221 0200 C 25 120 1022 P01 54 121 9610 58 221 0200 D 25 120 1023 C 53 121 9611 58 221 0500 E 25 120 1024 H 53 121 9612 58 221 1000 F 25 120 1025 D 53 121 9613 58 240 0005 A 26 120 1026 E 53 121 9614 58 240 0005 G 26 120 1028 F 53 121 9615 58 240 0010 A 26 120 1031 A 53 123 2016 60 240 0010 B 26 120 1032 G 53 123 2019 60 240 0010 G 26 120 1033 B 53 124 1001 59 240 0020 B 26 120 1034 H 53 124 2001 59 240 0020 H 26 120 1035 C 53 150 0006 BG 42 240 0025 P01 26 120 1036 D 53 220 0002 A 25 240 0050 B 26 120 1037 E 53 220 0002 AG 25 240 0050 C 26 120 1038 F 53 220 0005 A 25 240 0050 H 26 120 1100 52 220 0005 G 25 240 0100 C 26 120 1101 52 220 0010 A 25 240 0200 D 26 120 1102 52 220 0010 B 25 290 0002 A 26 120 1103 52 220 0010 G 25 290 0005 A 26 120 1104 52 220 0020 B 25 290 0010 A 26 120 1200 46 54 220 0020 C 25 290 0010 B 26 120 1201 46 54 220 0020 H 25 290 0010 C 26 120 1202 46 54 220 0025 P01 25 290 0020 B 26 120 2040 P01 46 220 0025 R 25 290 0020 H 26 120 2040 R 46 220 0050 B 25 290 0025 P01 26 120 2040 T 46 220 0050 BG 25 290 0025 R 26 121 0001 57 60 220 0050
29. 100 mg 1 mL 100 906 0010 G ISOLUTE HCX 5 100 mg 10 mL 50 906 0013 H ISOLUTE HCX 5 130 mg 10 mL 50 ISOLUTE 96 HCX 5 Fixed Well Plates Part Number Description Qty 906 0025 P01 ISOLUTE 96 HCX 5 25 mg plate 1 906 0100 P01 ISOLUTE 96 HCX 5 100 mg plate 1 ISOLUTE Array HCX 5 Loose Wells Part Number Description Qty 906 0025 R ISOLUTE Array HCX 5 25 mg 1 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE HCX 5 TN116 Generic Method for the Extraction of Basic Drugs from Biological Fluids using ISOLUTE Mixed mode SPE Columns and 96 well Plates SOLUTE Columns and Plates ISOLUTE HCX Q ISOLUTE HCX Q combines C8 Octyl and carboxylic Si O i CH COO acid functionalities Si O i CH CH Average particle size 50 um Pore diameter 60 A Sorbent Type Mixed Mode Application Extraction of quaternary amine and polybasic analytes from aqueous matrix using dual non polar and vveak cation exchange interactions ISOLUTE HCX Q SPE Columns Part Number Description Qty 986 0002 A ISOLUTE HCX Q 25 mg 1 mL 100 986 0005 A ISOLUTE HCX Q 50 mg 1 mL 100 986 0010 A ISOLUTE HCX Q 100 mg 1 mL 100 ISOLUTE 96 HCX Q Fixed Well Plates Part Number Description Qty 986 0025 P01 ISOLUTE 96 HCX Q 25 mg plate 1 986 0100 P01 ISOLUTE 96 HCX Q 100 mg plate 1 ISOLUTE Array HCX Q Loose Wells Part Num
30. 23 Fax 81 3 5627 3121 jp_order biotage com China Tel 86 21 2898 6655 Fax 86 21 2898 6153 cn_order biotage com www biotage cn Distributors Please visitour Web site at www biotage com for contact details Orders can also be placed using your VISA or MasterCard account and American Express IN THE US ONLY 2015 Analytical Sample Preparation Catalog Biotage 2015 Product Quality Biotage is committed to developing and manufacturing sample preparation products ofthe highest quality State of the art manufacturing techniques are supported by a comprehensive Quality Control QC testing program documented under our 1509001 2008 registered Quality Management System All the components used to make our sample preparation products tubes frits 96 well plates and SPE sorbents are rigorously cleaned and QC tested to ensure they meet our demanding purity specifications We use sophisticated instrumental techniques to confirm the physical and chemical nature of every batch of SPE sorbent ensuring reproducible performance in your application Every ISOLUTE or EVOLUTE SPE product is accompanied by a detailed Quality Assurance QA report for your reference This page explains the importance of the information it contains and the impact this has on every sample preparation procedure you perform Reproducible sorbent mass packed into SPE columns e Column capacity and analyte elution volume requirements are c
31. 28 0145 B 928 0500 E 9290 0100 32 32 32 32 32 32 32 32 32 32 32 32 32 30 30 30 30 30 30 30 31 31 31 31 31 31 31 31 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 18 44 44 39 39 39 39 39 39 39 44 9291 0100 9320 0100 9321 0100 933 0050 B 933 0100 C 934 0040 C 935 0040 C 941 0020 B 9460 0100 9470 0100 9480 0100 9500 0100 9503 0100 9520 0100 9532 0100 9533 0100 9712 0100 976 0400 C 9800 1000 9800 5000 986 0002 A 986 0005 A 986 0010 A 986 0025 P01 986 0025 R 986 0100 P01 C103186 C103187 C103188 C103189 C103190 C103192 C103193 C103194 C103196 C103197 C103198 C103199 C103200 C103201 C103202 C103203 C103263 C103264 C109301 C112175 C125713 C133718 C133968 C44139 C44282 C44283 C44577 C44880 C45269 C45286 C47818 C47820 C48928 C48929 C48950 C50000 C50974 C50976 44 44 44 39 39 39 39 39 44 44 44 44 44 44 44 44 44 40 44 44 31 31 31 31 31 31 67 67 67 67 67 67 67 67 67 67 67 67 67 67 67 67 67 67 67 67 63 67 63 67 67 67 67 67 67 67 67 67 67 67 67 63 63 63 C52006 C52689 C55941 C56536 C56786 C58309 C59546 C61345 M01 0002 G MO2 0002 B MO2 0002 G M06 0002 B M06 0002 G 08 0002 10 0002 10 0002 18 0002 M18 0002 G M21 0005 B M21 0005 G M28 0002 B M34 0005 B M69 0002 B M72 0002 B M72 0002 G M73 0002 B M73 0010 H PP
32. 48 and 96 VVell Plate Fixed VVell and Array Plate Dimensions of both 96 vvell plates fixed vvell and Array format and 48 vvell plates are shovvn above Orange outlines shovv the distribution of the wells in the 48 vvell format grey squares represent the 96 well format As with individual columns the last character s of the part number identifies the format column type The suffix for 96 vvell plates S 1 48 vvell plates is Q01 and pre assembled Array plates is RP 1 mL and TP 2 mL Scale diagrams 60 of actual size of the 96 vvell and 48 vvell fixed well plates and the pre assembled Array plate are shown below Note the skirt height distance from well outlet to sealing edge differs between plate types To prevent cross talk when processing plates well outlets should penetrate the collection plate correctly To accommodate the skirt height difference the VacMaster 96 is supplied complete with a spacer for use with the Array format This spacer is not required for processing 96 well or 48 well plates Total height 1 mL R 44 mm eee eaee aes TUUUUUUUUUUT Side elevation 60 of actual size of populated Array plate bottom 96 well plate top left and 48 well plate top
33. Analytical Sample Preparation 2015 Catalog i e 18 Biotage CD Biotage CD Analytical Sample Preparation 2015 Catalog Contents Biotage 1 Point Support 3 Sample Preparation Format Options 9 ISOLUTE SLE Supported Liquid Extraction 13 Columns and Plates ISOLUTE PLD Protein and Phospholipid Removal Plates 17 EVOLUTE Sample Preparation Products 19 ISOLUTE SPE Columns and 96 Well Plates 23 ISOLUTE Myco SPE Columns 41 ISOLUTE Bulk SPE Sorbents 43 ISOLUTE Protein Precipitation Products 45 ISOLUTE QuEChERS 47 AFFINILUTE MIP Molecularly Imprinted Polymers 49 Sample Preparation Accessories 51 Manual Sample Processing Products and Accessories 7 Biotage PRESSURE 48 and PRESSURE 96 Biotage VacMaster 10 and 20 Biotage VacMaster 96 Sample Processing Accessories Biotage Gravity Rack Automated Sample Processing Products 61 Biotage Extrahera RapidTrace Evaporation 65 Biotage SPE Dry 96 and 96 Dual TurboVap Index 68 Part Number Index 70 2015 Analytical Sample Preparation Catalog Biotage 2015 Biotage 1 Point Support The Answer to All Your Questions Customer Support and Resources Method Development and Troubleshooting Advice Biotage is ready to assist you vvith our team of Analytical Chemists who have many years of experience in providing practical theoretical and technical knovvledge relating to sample preparat
34. C 25 290 0050 B 26 121 0002 57 60 220 0050 H 25 290 0050 C 26 121 0003 57 60 220 0050 L 25 290 0050 P01 26 121 0004 57 60 220 0050 P01 25 290 0050 R 26 121 0005 57 220 0050 R 25 290 0100 C 26 121 0009 57 60 220 0100 B 25 290 0100 P01 26 121 0009 S 57 220 0100 C 25 290 0100 T 26 121 0010 57 220 0100 CG 25 291 0010 A 27 2015 Analytical Sample Preparation Catalog Biotage 2015 291 0010 B 291 0010 G 291 0020 B 291 0025 P01 291 0025 R 291 0050 B 291 0050 C 291 0050 P01 291 0100 C 291 0100 P01 291 0200 D 291 0500 E 320 0002 A 320 0005 A 320 0010 A 320 0010 B 320 0010 G 320 0020 B 320 0025 P01 320 0025 R 320 0050 B 320 0050 C 320 0050 P01 320 0100 C 320 0200 D 321 0005 A 321 0010 A 321 0010 B 321 0010 G 321 0020 B 321 0025 P01 321 0025 R 321 0050 B 321 0050 C 321 0050 H 321 0100 C 321 0100 T 360 0002 A 360 0005 A 360 0010 A 360 0010 B 360 0025 P01 360 0050 B 360 0050 C 360 0050 P01 360 0100 C 360 0100 P01 360 0100 T 390 0010 A 390 0010 B 390 0020 B 390 0025 P01 390 0025 R 390 0050 B 390 0050 C 390 0100 C 413640SP 414045SP 414169SP 414174SP 414201SP 414203SP 27 27 27 27 27 27 27 27 27 27 27 27 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 29 29 29 29 29 29 29 29 29 29 29 27 27 27 27 27 27 27 27 62 62 62 62 62 62 414253SP 414254SP 414255SP 414256SP 414257SP 420 0010 A 420 0010 B 420 0020 B 420 0025 P01
35. C ISOLUTE PAH 1 5 g 6 mL 30 927 0150 CD ISOLUTE PAH 1 5 g 6 mL Depth filter 30 ISOLUTE SAX PSA Columns Remove polar interferences and pigments from plant extracts prior to pesticide residue analysis Download application note IST1027A for more information Part Number Description Qty 924 0050 C ISOLUTE SAX PSA 500 mg 6 mL 30 924 0100 C ISOLUTE SAX PSA 1 g 6 mL 30 ISOLUTE TPH SPE Columns Extract total petroleum hydrocarbons from water samples and eliminate tedious shaking and emulsion problems common in liquid liquid extraction methods Manual and automated methodologies are available download application notes 1571042 and IST1018 respectively for more information Part Number Description Qty 752 0100 C ISOLUTE TPH 1 g 6 mL 30 2 40 SOLUTE Columns and Plates ISOLUTE O amp G SPE columns ISOLUTE Na S0 FL SPE columns Remove halogenated interferences from mineral oil extracts Application note IST1077 complies with the 1509377 2 200 method Extract total Oil and Grease from water samples and fractionate into HEM and SGT HEM fractions in one step Application note IST1005 meets US EPA 1664 guidelines Part Number Description Qty 976 0400 C ISOLUTE Na2SO4 FL 4 g 6 mL 30 Part Number Description Qty 753 0100 CD ISOLUTE O amp G 1 g 6 mL 30 753 0300 FD TSOLUTE O amp G 3 g 70 mL 16 2015 Analytical Sample Preparation Catalog Biotage 2015 SOLUTE Myco SPE Columns Solid Phase Extraction Column
36. CBA SPE Columns for the Extraction of Aqueous Samples 530 0200 D ISOLUTE SCX 2 4 15 mL 20 ISOLUTE 96 SCX Fixed Well Plates Part Number Description Qty 530 0025 P01 ISOLUTE 96 SCX 25 mg plate 1 530 0050 P01 ISOLUTE 96 SCX 50 mg plate 1 530 0100 P01 ISOLUTE 96 SCX 100 mg plate 1 ISOLUTE Array SCX Loose Wells Part Number Description Qty 530 0050 R ISOLUTE Array SCX 50 mg 1 mL wells 100 530 0100 T ISOLUTE Array SCX 100 mg 2 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE SCX TN106 Method Development in Solid Phase Extraction using ISOLUTE SCX and SCX 3 SPE Columns for the Extraction of Aqueous Samples 35 SOLUTE Columns and Plates ISOLUTE SCX 2 E Si OH Chemical structure Of propylsulfonic acid bonded to the surface of a Silica particle i o i cH so H functional group covalently Average particle size 50 pm Pore diameter Sorbent Type Exchange capacity 60A Strong cation exchange 0 6 meq g Application Extraction of basic analytes from aqueous or partially aqueous sample matrix Supplied in the protonated form Sorbent of choice if strong cation exchange is primary retention mechanism or if aqueous conditions required for analyte elution For methods listing PRS as the SPE sorbent SCX 2 is a direct replacement ISOLUTE SCX 2 SPE Columns Part Num
37. Columns Part Number Description Qty Part Number Description Qty 603 0002 A EVOLUTE AX 25 mg 1 mL 100 602 0002 A EVOLUTE VVCX 25 mg 1 mL 100 603 0002 AG EVOLUTE AX 25 mg 1 mL Tabless 100 EVOLUTE VVCX 50 um SPE Columns EVOLUTE AX 50 um SPE Columns 612 0005 B EVOLUTE VVCX 50 mg 3 mL 50 613 0005 B EVOLUTE AX 50 mg 3 mL 50 612 0010 B EVOLUTE VVCX 100 mg 3 mL 50 613 0010 B EVOLUTE AX 100 mg 3 mL 50 612 0010 H EVOLUTE VVCX 100 mg 10 mL XL 50 613 0010 BG EVOLUTE AX 100 mg 3 mL Tabless 50 612 0020 C EVOLUTE VVCX 200 mg 6 mL 30 613 0010 H EVOLUTE AX 100 mg 10 mL XL 50 612 0050 C EVOLUTE VVCX 500 mg 6 mL 30 613 0020 C EVOLUTE AX 200 mg 6 mL 30 612 0050 CG EVOLUTE VVCX 500 mg 6 mL Tabless 30 vt EVOLUTE EXPRESS VVCX 30 um Fixed Well Plates EVOLUTE EXPRESS AX 30 um Fixed Well Plates 10 mg Fixed Well Plate 603 0010 PXO1 EVOLUTE EXPRESS AX 1 602 0030 PX01 EVOLUTE EXPRESS WCX 1 10 mg Fixed Well Plate 30 mg Fixed Well Plate 603 0030 PX01 EVOLUTE EXPRESS AX 1 EVOLUTE WCX 30 um Array Loose Wells 30 mg Fixed Well Plate EVOLUTE AX 30 um Array Loose Wells 603 0025 R EVOLUTE Array AX 25 mg 1 mL Wells 100 602 0025 R EVOLUTE Array WCX 25 mg 1 mL Wells 100 EVOLUTE EXPRESS products can be used with the Load VVash Elute procedure See www biotage com for more information Tabless columns for use with PRESSURE Positive Pressure Manifolds and other automated SPE systems Other tabless columns are available contact Biotage for details 21 EVOLUTE
38. Drugs from Biological Fluids using Non polar Non endcapped Sorbents TN126 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Biological Fluid Samples ISOLUTE C2 EC Chemical structure of C2 silane and trimethyl silyl group covalently bonded VW 51 0 51 CH CH to the surface of a silica O particle Average particle size 50 um Pore diameter 60 Sorbent Type Non Polar Application Extraction from aqueous matrix using non polar interactions for acidic neutral and basic compounds ISOLUTE C2 EC SPE Columns Part Number Description Qty 321 0005 A ISOLUTE C2 EC 50 mg 1 mL 100 321 0010 A ISOLUTE C2 EC 100 mg 1 mL 100 321 0010 B ISOLUTE C2 EC 100 mg 3 mL 50 321 0010 G ISOLUTE C2 EC 100 mg 10 mL 50 321 0020 B ISOLUTE C2 EC 200 mg 3 mL 50 321 0050 B ISOLUTE C2 EC 500 mg 3 mL 50 321 0050 C ISOLUTE C2 EC 500 mg 6 mL 30 321 0050 H ISOLUTE C2 EC 500 mg 10 mL 50 321 0100 C ISOLUTE C2 EC 1 g 6 mL 30 ISOLUTE 96 C2 EC Fixed Well Plates Part Number Description Qty 321 0025 1 ISOLUTE 96 C2 EC 25 mg plate 1 ISOLUTE Array C2 EC Loose Wells Part Number Description Qty 321 0025 R ISOLUTE Array C2 EC 25 mg 1 mL wells 100 321 0100 T ISOLUTE Array C2 EC 100 mg 2 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE C2 EC TN101 Method De
39. E WAX 30 um SPE Columns Part Number Description Qty 650 0010 PX01 EVOLUTE EXPRESS 10 mg 1 Sorbent Selection Plate 650 0030 PX01 EVOLUTE EXPRESS 30 mg l Sorbent Selection Plate EVOLUTE User Guide For further information dovvnload the EVOLUTE User Guide from vvvvvv biotage com Literature part number EVO UG_2012 rev 1 EVOLUTE User Guide Biotage Biotage are constantly developing new applications on EVOLUTE and EVOLUTE EXPRESS products Visit www biotage com for the latest information EVOLUTE EXPRESS products can be used with the Load VVash Elute procedure See www biotage com for more information 2015 Analytical Sample Preparation Catalog Biotage 2015 ISOLUTE SPE Columns and 96 Well Plates Sorbents and Formats for a Wide Range of Sample Preparation Applications 24 SOLUTE Columns and Plates ISOLUTE ENV OH Chemical structure of rl ISOLUTE ENV a hydroxylated polystyrene divinylbenzene co polymer OH CH 2 5 cc N A DO ge Gi C Ch gair CH CH o Average particle size 110 um Pore diameter 800 A Sorbent Type Non Polar Application Extraction of very polar compounds that are not retained by C8 and C18 non polar silica based sorbents ISOLUTE ENV SPE Columns Part Number Description Qty 915 0001 A ISOLUTE ENV 10 mg 1 mL 100 915 0002 A ISOLUTE ENV 25 mg 1 mL 100 915 0002 G ISOLUTE ENV 25 mg 10 mL 50 915 0005 A ISOLUTE ENV 50
40. HL Sample Volume 500 820 0055 BG 500 ISOLUTE SLE 400 uL Sample Volume 500 tabless 820 0140 C 1000 TSOLUTE SLE 1 mL Sample Volume 1000 820 0140 CG 1000 ISOLUTE SLE 1 mL Sample Volume 1000 tabless 820 0290 0 1000 TSOLUTE SLE 2 mL Sample Volume 1000 Biotage Biotage are constantly developing new applications on ISOLUTE SLE products Visit www biotage com for the latest information 15 16 2015 Analytical Sample Preparation Catalog Biotage 2015 SOLUTE PLD Protein and Phospholipid Removal Plates Simple Effective Sample Clean up for LC MS MS SOLUTE PLD Effortlessly Improve Analyte Sensitivity ISOLUTE PLD Protein and Phospholipid Removal Plates ISOLUTE PLD Protein and Phospholipid Removal Plates provide a very effective but extremely simple sample clean up for LC MS MS analysis Requiring next to no method development ISOLUTE PLD can be integrated quickly and easily into routine workflow increasing productivity and reducing instrument downtime ISOLUTE PLD plates remove gt 99 of plasma proteins and phospholipids the main causes of ion suppression leading to cleaner extracts and increased sensitivity signal to noise S N for a broad range of analytes Step 1 Dispense crash solvent M Phospholipids Protein A Analyte s Typical ISOLUTE PLD procedure Using a simple solvent crash filtration based procedure proteins and phospholipids are simultaneously re
41. L 50 712 0050 L ISOLUTE FL 500 mg 6 mL Glass 30 712 0100 B SOLUTE FL 1 9 3 mL 50 712 0100 C ISOLUTE FL 1 9 6 mL 30 712 0200 D ISOLUTE FL 2 g 15 mL 20 712 0500 E ISOLUTE FL 5 g 25 mL 20 712 2000 F ISOLUTE FL 20 g 70 mL 16 Support Documents for ISOLUTE FL IST1080 Multi residue Extraction and Clean up of Pesticides from Fruits and Vegetables ISOLUTE HM N Columns for Supported Liquid Extraction Part Number Description Qty 800 0040 BM ISOLUTE HM N 300 uL sample 100 800 0100 CM TSOLUTE HM N 1 mL sample 100 800 0220 DM TSOLUTE HM N 3 mL sample 100 800 0350 EM TISOLUTE HM N 5 mL sample 100 800 0700 FM TSOLUTE HM N 10 mL sample 50 800 1300 FM TSOLUTE HM N 20 mL sample 50 Tabless columns for use with Pressure Positive Pressure Manifolds and other automated SPE systems Other tabless columns are available contact Biotage for details ISOLUTE Alumina High activity 50 200 um particle size range alumina available in acidic neutral and basic surface pH options The surface of the alumina can absorb molecules by interaction with the aluminum metal center hydrogen bonding with surface hydroxyl groups or by ion exchange if the surface carries a charge The extent of these different interactions can be enhanced by control of the surface pH by treatment with acidic basic or neutral solutions ISOLUTE AL A Acid washing results in a surface with decreased capacity for basic compounds Compounds are retained by io
42. M 48 PPM 96 PPM A48 1232 PPM A48 1275 PPM A48 13100 PPM A48 16100 PPM A48 1RCK PPM A48 3RCK PPM A48 6RCK PPM A48 GSKT PPM A48 WST PPM A96 1024 PPM A96 GSKT PPM GA Q0000 50V Q0010 15V Q0020 15V Q0030 15V Q0035 15V Q0050 15V Q0060 15V Q0070 15V Q0080 15V Q0090 15V SD2 9600 DHS EU SD2 9600 DHS NA 5D2 9600 DFHS T EU SD2 9600 DHS T NA SD 9600 DHS EU SD 9600 DHS NA SD 9600 DHS T EU SD 9600 DHS T NA 63 63 67 63 63 63 67 67 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 56 56 56 56 56 56 56 56 56 56 56 56 56 56 48 48 48 48 48 48 48 48 48 48 66 66 66 66 66 66 66 66 74 Limited VVarranty Biotage warrants that Biotage Consumables including all FLASH FLASH ISOLUTE EVOLUTE and Samplet purification cartridges scavenger resins solid bound reagents and microwave vials will be of good quality and workmanship and will meet the applicable product specification This warranty applies only to the initial test performed at the Customer s facility upon the initial start up of the consumables and expires at the time the user applies an actual sample to the consumable If the cartridge is packed with media provided by the Customer the Biotage warranty applies only to the plastic tube frits and labor required for packing and testing the cartridge Biotage will not be liable for any damage to media provided by the Customer that may be caused when Biotage packs
43. TE Multimode 300 mg 6 mL 30 903 0020 C ISOLUTE HAX 200 mg 6 mL 30 904 0050 B ISOLUTE Multimode 500 mg 3 mL 50 903 0020 H ISOLUTE HAX 200 mg 10 mL 50 904 0100 C ISOLUTE Multimode 1 g 6 mL 30 ISOLUTE 96 HAX Fixed Well Plates Support Documents for ISOLUTE Multimode Part Number Description Qty i IST1022 Extracti Aflatoxi 903 0025 P01 ISOLUTE 96 HAX 25 mg plate 1 20 00 903 0100 1 ISOLUTE 96 HAX 100 mg plate 1 IST1076 Extraction of Acrylamide from Cooked Foodstuffs ISOLUTE Array HAX Loose Wells Part Number Description Qty 903 0025 R ISOLUTE Array HAX 25 mg 1 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose vvell part number Support Documents for ISOLUTE HAX TN127 Method Development in Solid Phase Extraction using ISOLUTE HAX for the Extraction of Drugs from Biological Fluid Samples 2015 Analytical Sample Preparation Catalog Biotage 2015 ISOLUTE NH2 SOLUTE Columns and Plates ISOLUTE PSA Chemical structure of NH2 aminopropyl silane covalently bonded to the surface ofa Chemical structure of PSA ethylenediamine n propyl Silane covalently bonded Si O Si OH 2 Si CH NH Average particle size 50 pm Pore diameter Sorbent Type Exchange capacity Application Extraction of strong acids and polyacidic 60 Weak anion exchange pKa 9 8 or polar 0 6 meq g silica particle compounds
44. V 1 g 25 mL 20 ISOLUTE ENV Fixed Well Plates Part Number Description Qty 915 0010 1 TSOLUTE 96 ENV 10 mg plate 1 915 0025 1 ISOLUTE 96 ENV 25 mg plate 1 ISOLUTE ENV Loose Wells Part Number Description Qty 915 0025 R ISOLUTE Array ENV 25 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE ENV TN109 Method Development in Solid Phase Extraction using ISOLUTE ENV SPE Columns for the Extraction of Aqueous Samples Tabless columns for use with PRESSURE Positive Pressure Manifolds and other automated SPE systems Other tabless columns are available contact Biotage for details 2015 Analytical Sample Preparation Catalog Biotage 2015 SOLUTE Columns and Plates ISOLUTE C18 ISOLUTE C18 EC Chemical structure of C18 Octadecyl endcapped Silane and trimethyl silyl group covalently bonded Chemical structure of C18 Octadecyl non endcapped Silane covalently bonded to the surface of a silica N SO CH CH AIO CH CH O particle O to the surface of a silica i LER particle Si OH 91 9 Average particle size 50 um Average particle size 50 um Pore diameter 60 A Pore diameter 60 A Sorbent Type Non Polar Sorbent Type Non Polar Application Most commonly used C18 sorbent for the extraction of acidic neutral and basic compounds from aqueous matrices Second
45. acid 96 well SPE plates Bulk sorbent SPE columns 24 3 11 14 15 11 54 54 51 54 52 52 49 38 11 54 61 43 28 27 27 26 27 44 26 27 25 43 25 52 35 44 35 CN Cyanopropyl 96 well SPE plates 29 SPE columns 29 Collection plates 96 well 54 Column adaptors 52 Column caps 52 Customer Support 4 Cyanopropyl see CN D Deep well collection plates 54 Depth filter reservoirs 52 DIOL 2 3 Dihydroxypropoxypropyl SPE columns 37 Drying adaptor VacMaster 59 E Empty reservoirs 53 ENV Hydroxylated polystyrene divinylbenzene 96 well SPE plates 24 SPE columns 24 EPH SPE columns 39 Ethyl see C2 C2 EC Ethylbenzene sulfonic acid see SCX 3 Evaporation 65 EVOLUTE 4 19 22 Extrahera SLE and SPE Automation System 62 F Filtration columns 55 Filtration plates 54 Florisil SPE columns 38 Frits polyethylene 53 G Gravity Rack 60 H HAX 96 well SPE plates 32 SPE columns 32 HCX 96 well SPE plates 30 SPE columns 30 HCX 3 96 well SPE plates 30 SPE columns 30 HCX 5 96 well SPE plates 31 SPE columns 31 HCX Q 96 well SPE plates 31 SPE columns 31 HM N diatomaceous earth Bulk material 44 Packed columns 38 Ion exchange bulk sorbents 44 Ion exchange SPE columns and 96 well plates 33 36 ISOLUTE 96 SPE plates 11 15 23 36 ISOLUTE Array wells and SPE plates 11 23 36 ISOLUTE Bulk sorbents 43 ISOLUTE EPH 39 ISOLUTE HM N diatomaceous earth Bulk sorbe
46. age particle size 50 um Pore diameter 60 Sorbent Type Polar Application Extraction of polar compounds from a non polar sample matrix using hydrogen bonding retention mechanism Less retentive sorbent than SI Can also be used as a non polar sorbent with aqueous sample matrices ISOLUTE DIOL SPE Columns Part Number Description Qty 430 0010 A ISOLUTE DIOL 100 mg 1 mL 100 430 0010 B ISOLUTE DIOL 100 mg 3 mL 50 430 0020 B ISOLUTE DIOL 200 mg 3 mL 50 430 0050 B ISOLUTE DIOL 500 mg 3 mL 50 430 0050 C ISOLUTE DIOL 500 mg 6 mL 30 430 0050 H ISOLUTE DIOL 500 mg 10 mL 50 430 0100 C ISOLUTE DIOL 1 g 6 ML 30 Support Documents for ISOLUTE DIOL TN102 Method Development in Solid Phase Extraction using Polar ISOLUTE SPE Columns for the Extraction of Non aqueous Samples 37 38 SOLUTE Columns and Plates ISOLUTE FL Average particle size 150 250 um Pore diameter 60 Nominal moisture content lt 2 Surface pH 8 Sorbent Type Polar Application Extraction of polar compounds from a non polar sample matrix Alternative to silica based polar sorbents Minimal retention of basic compounds Activated for separation of chlorinated pesticides ISOLUTE FL SPE Columns Part Number Description Oty 712 0010 A ISOLUTE FL 100 mg 1 mL 100 712 0020 B ISOLUTE FL 200 mg 3 mL 50 712 0050 B ISOLUTE FL 500 mg 3 mL 50 712 0050 BG ISOLUTE FL 500 mg 3 mL 50 Tabless 712 0050 C ISOLUTE FL 500 mg 6 mL 30 712 0050 H ISOLUTE FL 500 mg 10 m
47. analytes from aqueous or partially aqueous sample matrix Supplied in the protonated form Sorbent of choice where significant non polar secondary interactions are required ISOLUTE SCX 3 SPE Columns Part Number Description Qty 533 0002 A SOLUTE SCX 3 25 mg 1 mL 100 533 0005 A ISOLUTE SCX 3 50 mg 1 mL 100 533 0010 A ISOLUTE SCX 3 100 mg 1 mL 100 533 0010 B ISOLUTE SCX 3 100 mg 3 mL 50 533 0020 B ISOLUTE SCX 3 200 mg 3 mL 50 533 0050 B ISOLUTE SCX 3 500 mg 3 mL 50 533 0050 C SOLUTE SCX 3 500 mg 6 mL 30 533 0050 H ISOLUTE SCX 3 500 mg 10 mL 50 533 0100 B ISOLUTE SCX 3 1 g 3 mL 50 533 0100 C iSOLUTE SCX 3 1 0 6 mL 30 533 0200 D ISOLUTE SCX 3 2 g 15 mL 20 ISOLUTE 96 SCX 3 Fixed Well Plates Part Number Description Qty 533 0025 P01 ISOLUTE 96 SCX 3 25 mg plate 1 533 0050 P01 ISOLUTE 96 SCX 3 50 mg plate 1 533 0100 P01 ISOLUTE 96 SCX 3 100 mg plate 1 ISOLUTE Array SCX 3 Loose Wells Part Number Description Qty 533 0025 R 533 0100 T ISOLUTE Array SCX 3 25 mg 1 ML wells 100 ISOLUTE Array SCX 3 100 mg 2 mL vvells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE SCX 3 TN106 Method Development in Solid Phase Extraction using ISOLUTE SCX and SCX 3 SPE Columns for the Extraction of Aqueous Samples Tabless columns for use with PRESSURE Positive Pressure Manifolds and other automated SPE systems Other tabless columns are
48. are collected Select the correct ISOLUTE SLE product based on the volume of sample to be extracted see table 1 Extraction solvent volumes are also listed Table 1 Recommended sample and elution volumes for ISOLUTE SLE products Product Description Maximum Elution load volume protocol volume 200 uL 96 vvell plate 200 HL 1x LME 400 HL 96 vvell plate 400 uL 2 x 900 uL or 3 x 700 uL 1 mL 48 vvell plate 1 mL 5 X LME 200 HL Array well plate 200 uL 2x 600 HL 400 uL Array well plate 400 uL 3 x 750 uL 400 uL column 400 uL 2 x 900 uL 1 mL column 1 mL 2 x 2 5 mL 2 mL column 2 mL 2 65 mi 5 mL column 5 mL 3x8mL 10 mL column 10 mL 2 X 20 imi 2015 Analytical Sample Preparation Catalog Biotage 2015 Supported Liquid Extraction Achieving Simplicity and Success in Sample Preparation ISOLUTE SLE Supported Liquid Extraction Method Selection ACIDIC DRUG NEUTRAL DRUG BASIC DRUG Buffer 1 Buffer 2 Buffer 3 Buffer 4 1 v v formic acid 0 1 v v formic acid Water 0 5M ammonium hydroxide Mix plasma or urine sample 1 1 v v Mix plasma or urine sample 1 1 v v Mix plasma or urine sample Mix plasma or urine sample 1 1 v v with 1 formic acid aq v v with 0 1 formic acid aq v v 1 1 v v with water with 0 5M ammonium hydroxide Urine pHi 2 7 Plasma pH 3 5 Urine pH 4 0 Plasma pH 6 2 Urine pH 7 0 Plasma pH 8 2 Urine pH 10 8 Plasma pH 10 7 Extraction Solvent 1 Extraction Solvent 2 Extraction Solvent 3 E
49. ary silanol or ionic interactions can be used to enhance extract purity and method robustness for basic compounds ISOLUTE Ci8 SPE Columns Application Extraction of polar compounds from aqueous sample matrices ISOLUTE C18 EC SPE Columns Part Number Description Qty Part Number Description Qty 220 0002 A ISOLUTE C18 25 mg 1 mL 100 221 0002 A ISOLUTE C18 EC 25 mg 1 mL 100 220 0002 AG ISOLUTE C18 25 mg 1 mL Tabless 100 221 0005 A ISOLUTE C18 EC 50 mg i mL 100 220 0005 A ISOLUTE C18 50 mg 1 mL 100 221 0005 G ISOLUTE C18 EC 50 mg 10 mL 50 220 0005 G ISOLUTE C18 50 mg 10 mL 50 221 0010 A ISOLUTE C18 EC 100 mg 1 mL 100 220 0010 A ISOLUTE C18 100 mg 1 mL 100 221 0010 B ISOLUTE C18 EC 100 mg 3 mL 50 220 0010 B ISOLUTE C18 100 mg 3 mL 50 221 0010 C ISOLUTE C18 EC 100 mg 6 mL 30 220 0010 G ISOLUTE C18 100 mg 10 mL 50 221 0010 G ISOLUTE C18 EC 100 mg 10 mL 50 220 0020 B ISOLUTE C18 200 mg 3 mL 50 221 0020 B ISOLUTE C18 EC 200 mg 3 mL 50 220 0020 C ISOLUTE C18 200 mg 6 mL 30 221 0020 C ISOLUTE C18 EC 200 mg 6 mL 30 220 0020 H ISOLUTE C18 200 mg 10 mL 50 221 0020 H ISOLUTE C18 EC 200 mg 10 mL 50 220 0050 B ISOLUTE C18 500 mg 3 mL 50 221 0050 B ISOLUTE C18 EC 500 mg 3 mL 50 220 0050 BG ISOLUTE C18 500 mg 3 mL Tabless 50 221 0050 BS TSOLUTE C18 EC 500 mg 3 mL 50 220 0050 C ISOLUTE C18 500 mg 6 mL 30 221 0050 C ISOLUTE C18 EC 500 mg 6 mL 30 220 0050 H ISOLUTE C18 500 mg 10 mL 50 221 0050 CD ISOLUTE C18 EC 500 mg 6 mL 30 220 0050 L ISOLUTE
50. ation exchange Application Extraction of basic analytes from aqueous or partially aqueous sample matrix Supplied in the protonated form ISOLUTE SCX SPE Columns Part Number Description Qty 530 0005 A ISOLUTE SCX 50 mg 1 mL 100 530 0010 A ISOLUTE SCX 100 mg 1 mL 100 530 0010 B ISOLUTE SCX 100 mg 3 mL 50 530 0020 B ISOLUTE SCX 200 mg 3 mL 50 530 0050 B ISOLUTE SCX 500 mg 3 mL 50 530 0050 C ISOLUTE SCX 500 mg 6 mL 30 530 0050 H ISOLUTE SCX 500 mg 10 mL 50 530 0100 B ISOLUTE SCX 1 g 3 mL 50 530 0100 C ISOLUTE SCX 1 g 6 mL 30 520 0002 A ISOLUTE CBA 25 mg 1 mL 100 520 0005 A ISOLUTE CBA 50 mg 1 mL 100 520 0010 A ISOLUTE CBA 100 mg 1 mL 100 520 0010 B ISOLUTE CBA 100 mg 3 mL 50 520 0020 B ISOLUTE CBA 200 mg 3 mL 50 520 0050 B ISOLUTE CBA 500 mg 3 mL 50 520 0050 C ISOLUTE CBA 500 mg 6 mL 30 520 0100 C ISOLUTE CBA 1 g 6 mL 30 520 0200 D ISOLUTE CBA 2 g 15 mL 20 ISOLUTE 96 CBA Fixed Well Plates Part Number Description Qty 520 0025 1 ISOLUTE 96 CBA 25 mg plate 1 520 0050 1 ISOLUTE 96 CBA 50 mg plate 1 520 0100 P01 ISOLUTE 96 CBA 100 mg plate 1 ISOLUTE Array CBA Loose Wells Part Number Description Qty 520 0025 R ISOLUTE Array CBA 25 mg 1 mL wells 100 520 0050 R ISOLUTE Array CBA 50 mg 1 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE CBA TN108 Method Development in Solid Phase Extraction using ISOLUTE
51. ber Description Qty 986 0025 R ISOLUTE Array HCX Q 25 mg 1 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose vvell part number Support Documents for ISOLUTE HCX Q TN129 Generic Method for the Extraction of Quaternary Amine and Polybasic Drugs from Biological Fluids using ISOLUTE HCX Q SPE Columns and 96 well Plates 31 SOLUTE Columns and Plates o ISOLUTE HAX ISOLUTE Multimode ISOLUTE HAX combines ISOLUTE Multimode C8 Octyl and quaternary i combines C18 Octadecyl _ amine functionalities 5 quaternary amine and ua CH CI 4 sulfonic acid functionalities Si O Si R SO H Average particle size 50 um Average particle size 50 um Pore diameter 60 A Pore diameter 60 Sorbent Type Mixed Mode Sorbent Type Mixed Mode Application Extraction of acidic analytes from aqueous matrix Application Isolation of small neutral highly water soluble using dual non polar and strong anion exchange interactions species from complex mixtures Suitable for a broad range of acidic compounds ISOLUTE HAX SPE Columns ISOLUTE Multimode SPE Columns Part Number Description Qty Part Number Description Qty 903 0005 A ISOLUTE HAX 50 mg 1 mL 100 904 0010 A ISOLUTE Multimode 100 mg 1 mL 100 903 0010 A ISOLUTE HAX 100 mg 1 mL 100 904 0030 B ISOLUTE Multimode 300 mg 3 mL 50 903 0020 B ISOLUTE HAX 200 mg 3 mL 50 904 0030 C ISOLU
52. ber Description Qty 532 0002 A ISOLUTE SCX 2 25 mg 1 mL 100 532 0005 A ISOLUTE SCX 2 50 mg 1 mL 100 532 0010 A ISOLUTE SCX 2 100 mg 1 mL 100 532 0010 B ISOLUTE SCX 2 100 mg 3 mL 50 532 0020 B ISOLUTE SCX 2 200 mg 3 mL 50 532 0050 B ISOLUTE SCX 2 500 mg 3 mL 50 532 0050 C ISOLUTE SCX 2 500 mg 6 mL 30 532 0050 H ISOLUTE SCX 2 500 mg 10 mL 50 532 0100 B ISOLUTE SCX 2 1 g 3 mL 50 532 0100 C ISOLUTE SCX 2 1 g 6 mL 30 532 0100 CG ISOLUTE SCX 2 1 g 6 mL Tabless 30 532 0200 D ISOLUTE SCX 2 2 g 15 mL 20 ISOLUTE 96 SCX 2 Fixed Well Plates Part Number Description Qty 532 0025 1 ISOLUTE 96 SCX 2 25 mg plate 1 532 0050 P01 ISOLUTE 96 SCX 2 50 mg plate 1 532 0100 1 ISOLUTE 96 SCX 2 100 mg plate 1 ISOLUTE Array SCX 2 Loose Wells Part Number Description Qty 532 0025 R ISOLUTE Array SCX 2 25 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE SCX 2 TN107 Method Development in Solid Phase Extraction using ISOLUTE SCX 2 SPE Columns for the Extraction of Aqueous Samples ISOLUTE SCX 3 So nal Si O si CH SO H O Si OH Average particle size 50 um Pore diameter 60 A Sorbent Type Exchange capacity 0 6 meq g Chemical structure of ethylbenzene sulfonic acid functional group covalently bonded to the surface of a Silica particle Strong cation exchange Application Extraction of basic
53. ber Description Qty 905 0002 A ISOLUTE HCX 3 25 mg 1 mL 100 905 0005 A ISOLUTE HCX 3 50 mg 1 mL 100 905 0010 A ISOLUTE HCX 3 100 mg 1 mL 100 ISOLUTE 96 HCX 3 Fixed Well Plates Part Number Description Qty 905 0025 P01 ISOLUTE 96 HCX 3 25 mg plate 1 905 0100 P01 ISOLUTE 96 HCX 3 100 mg plate 1 ISOLUTE Array HCX 3 Loose Wells Part Number Description Qty 905 0025 R ISOLUTE Array HCX 3 25 mg 1 mL wells 100 905 0100 T ISOLUTE Array HCX 3 100 mg 2 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE HCX 3 TN116 Generic Method for the Extraction of Basic Drugs from Biological Fluids using ISOLUTE Mixed mode SPE Columns and 96 Well Plates 2015 Analytical Sample Preparation Catalog Biotage 2015 ISOLUTE HCX 5 ISOLUTE HCX 5 combines C4 Butyl and sulfonic acid N l R SO functionalities PI OT SI CH CH Average particle size 50 um Pore diameter 60 Sorbent Type Mixed Mode Application HCX 5 provides the cleanest extract of all the mixed mode sorbents Ideal choice where the basic analyte to be extracted has sufficient non polar character to be vvell retained by the C4 non polar component of the mixed mode sorbent ISOLUTE HCX 5 SPE Columns Part Number Description Qty 906 0002 A ISOLUTE HCX 5 25 mg 1 mL 100 906 0005 A ISOLUTE HCX 5 50 mg 1 mL 100 906 0010 A ISOLUTE HCX 5
54. e or imprint vvhich is sterically and chemically complementary to a particular analyte or class of analytes The interactions mimic antibody or receptor binding and are stronger than interactions obtained vvith conventional separation materials Part Number Description M01 0002 G MO2 0002 B MO2 0002 G M06 0002 B M06 0002 G M08 0002 G M10 0002 B M10 0002 G M18 0002 B M18 0002 G M21 0005 B M21 0005 G M28 0002 B M34 0005 B M69 0002 B M72 0002 B M72 0002 G M73 0002 B M73 0010 H AFFINILUTE MIP Clenbuterol 25 mg 10 mL AFFINILUTE MIP Beta agonists 25 mg 3 mL AFFINILUTE MIP Beta agonists 25 mg 10 mL AFFINILUTE MIP NNAL 25 mg 3 mL AFFINILUTE MIP NNAL 25 mg 10 mL AFFINILUTE MIP Triazines 25 mg 10 mL AFFINILUTE MIP Chloramphenicol 25 mg 3 mL AFFINILUTE MIP Chloramphenicol 25 mg 10 mL AFFINILUTE MIP Beta blockers 25 mg 3 mL AFFINILUTE MIP Beta blockers 25 mg 10 mL AFFINILUTE MIP TSNAs 50 mg 3 mL AFFINILUTE MIP TSNAs 50 mg 10 mL AFFINILUTE MIP Amphetamines 25 mg 3 mL AFFINILUTE MIP Nitroimidazoles 50 mg 3 mL AFFINILUTE MIP Fluoroquinolones 25 mg 3 mL AFFINILUTE MIP NSAIDs 25 mg 3 mL AFFINILUTE MIP NSAIDs 25 mg 10 mL AFFINILUTE MIP PAH 25 mg 3 mL AFFINILUTE MIP PAH 10 mL 100 mg For more information visit www biotage com Qty 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 50 2015 Analytical Samp
55. e sealing cap Part Number Description Qty 121 5202 Collection plate 1 mL 50 121 5203 Collection plate 2 mL 50 Array Accessories Populate the reusable base plate to transfer individual Array wells into microplate format Seal any unused positions with base plate plugs Align wells and depopulate base plate after use with the well removing tool Use Luer adaptors to process individual Array wells using a VacMaster 10 or 20 vacuum manifold Part Number Description Qty 120 1000 1 ISOLUTE Array base plate 1 120 1200 ISOLUTE base plate sealing strips 50 strips of 8 120 1201 Luer Adaptors 25 to fit any vacuum manifold 120 1202 Well removing tool 1 2015 Analytical Sample Preparation Catalog Biotage 2015 Manual Sample Processing Products and Accessories 56 Biotage PRESSURE 48 and PRESSURE 96 Positive Pressure Manifolds Biotage PRESSURE 48 and PRESSURE 96 Biotage PRESSURE manifolds deliver positive pressure parallel processing for 96 well plates 1 mL 3 mL and 6 mL column formats The systems utilize a consistent uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns Biotage PRESSURE 96 Part Number PPM 96 Accessories PPM A96 GSKT PPM A96 1024 Description PRESSURE 96 Positive Pressure Manifold 96 position Includes 1 x 1 mL 96 well collection plate 1 x 2 mL 96 well collection plate 1 x 10 mL 24 well c
56. e 5m 5 00 mg 00 mg 00 mg EN VVaxed Fruit and Vegetables 15V 1 L 2 1 900 150 Q0060 15 Cleah up Tube 5m 5 50 mg mg mg AOAC Pigmented Fruit and T 2 4 12 4 Q0070 15V Vedetsbles Clean up Tube 15 mL 5 00 mg 00 mg 00 mg EN Pigmented Fruit and Vegetables 15V 1 L 2 150 900 15 Q0080 15 Clean up Tube 5m 5 mg mg mg EN Highly Pigmented Fruit and 1 L 2 1 4 un Vegetables Clean up Tube eal 7 Centrifuge Tubes Part Number Description Tube Pack Size 50 mL Centrifuge Tube Q0000 50V with Rack 50 mL 25 2015 Analytical Sample Preparation Catalog Biotage 2015 AFFINILU TE MIP Molecularly Imprinted Polymers Biotage 2015 49 50 AFFINILUTE MIP Molecularly Imprinted Polymers AFFINILUTE MIP Biotage AFFINILUTE MIP products offer analytical chemists ultimate selectivity compared with standard solid phase extraction techniques Unlike other methods AFFINILUTE MIP products rely on the specific molecular structure of the analyte for targeted capture resulting in exceptional clean up AFFINILUTE MIP columns combine high affinity with standard flow through sample preparation methodology Functional monomers Crosslinker Pr Az Formation of AS imprinting a cavities by polymerisation Template removal L Selective interaction Mechanism of MTP formation Unlike most separation particles that exhibit only non selective interactions MTP particles have a selective synthetic recognition sit
57. e available To order add the suffix P to the equivalent loose well part number add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE C18 EC Support Documents for ISOLUTE EC18 TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN126 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Drugs from Biological Fluid Samples Tabless columns for use with PRESSURE Positive Pressure Manifolds and other automated SPE systems Other tabless columns are available contact Biotage for details Column contains stainless steel frits SOLUTE Columns and Plates ISOLUTE MFC18 Chemical structure of CH monofunctional C18 silane covalently bonded to the pi OT Si CH CH surface of a silica particle CH SI OH Average particle size 50 pm Pore diameter 125A Sorbent Type Non Polar Application Extraction from aqueous matrix using both non polar interactions for acidic neutral and basic compounds and readily accessible secondary silanol or ionic interactions for basic compounds only Not the ideal ch
58. e packed columns containing ISOLUTE HM N see page 38 Inert Support modified diatomaceous earth Part Number Description Qty 9800 1000 ISOLUTE HM N bulk 1 kg 9800 5000 ISOLUTE HM N bulk 5 kg ISOLUTE HM N is available in additional quantities to those detailed in the above table Please contact your local Biotage representative 2015 Analytical Sample Preparation Catalog Biotage 2015 LA SOLUTE SORBENT C18 nn 40 70 100g gm LET TT Protein Precipitation Products Fast Simple Protein Removal SOLUTE PPT Protein Precipitation Plates 96 Well Plates The optimized filtration system in ISOLUTE PPT plates Be ee dr S Ser 120 2040 1 ISOLUTE PPT fixed well plate 2 mL 1 provides an easy to automate solution for efficient protein removal from biological fluid samples ISOLUTE Array Wells Part Number Description Qty 120 2040 R ISOLUTE Array PPT wells 1 mL 100 120 2040 T ISOLUTE Array PPT wells 2 mL 100 ISOLUTE Array Accessories Part Number Description Qty 120 1000 1 ISOLUTE Array base plate 100 120 1200 Strip of 8 base plate sealing plugs 100 120 1201 Luer adaptors to fit any standard sample 25 processing manifold 120 1202 VVell removing tool 1 Required when processing a partially populated ISOLUTE Array PPT plate Support Documents for ISOLUTE PPT TN130 Sample Preparation using ISOLUTE PPT Protein Precipitation Plates Apply crash Apply Precipitation A
59. e unit PTFE needle Stainless steel needle Stainless steel needle retainer Port sealing plugs PTFE stopcock pressure positive PTFE Stopcock Option and Spare Parts Qty 10 10 10 20 10 30 10 57 58 Biotage VacMaster 96 Sample Processing Manifold Biotage VacMaster 96 Vacuum manifold for processing 96 well SPE SLE or filtration plates Part Number Description Qty 121 9600 VacMaster 96 Sample Processing Manifold 1 without vacuum control Vacuum Control Options Part Number Description Qty 121 9601 VacMaster VCU 1 Vacuum Control Unit 1 121 9602 VacMaster VCU 2 Vacuum Control and 1 Generation Unit 96 Well Collection Plates Deep Well Part Number Description Qty 121 5202 Collection plate 1 mL 50 121 5203 Collection plate 2 mL 50 Replacement Parts Part Number Description Qty 121 9612 VacMaster 96 replacement gasket 1 121 9613 VacMaster 96 replacement o ring 1 121 9614 Collection plate spacer 2 mm i for deep well collection plates 121 9610 Array insert 6 mm acetal 1 121 9615 Collection plate spacer 29 mm 1 for shallovv vvell collection plates ni VacMaster 96 insert 12 mm for O j shallow skirt plates 2015 Analytical Sample Preparation Catalog Biotage 2015 Sample Processing Accessories Biotage VacMaster Drying Adaptor Connect to laboratory air or nitrogen supply to dry 10 or 20 SPE columns simul
60. ed with the Load Wash Elute procedure See www biotage com for more information Tabless columns for use with Pressure Positive Pressure Manifolds and other automated SPE systems Other tabless columns are available contact Biotage for details 2015 Analytical Sample Preparation Catalog Biotage 2015 EVOLUTE Advanced Polymeric Sorbents EVOLUTE AX EVOLUTE WCX All pH conditions COOH COO High pH L H CH2N CHs3 3 57 Chemical structure of EVOLUTE AX Chemical structure of EVOLUTE WCX Chemical Description Chemical Description Quaternary amine modified polystyrene divinylbenzene Carboxylic acid modified polystyrene divinylbenzene incorporating non ionizable hydroxyl groups incorporating non ionizable hydroxyl groups Average particle size 30 um 50 um Average particle size 30 um 50 um Pore diameter 40 Pore diameter 40 Sorbent Type Mixed mode non polar strong anion Sorbent Type Mixed mode non polar weak cation exchange exchange pKa 5 Exchange capacity 0 7 mmol g Exchange capacity 0 4 mmol g Application EVOLUTE AX can be used to extract acidic Application EVOLUTE WCX be used to extract strongly analytes from biological fluids and other aqueous matrices basic analytes e g quaternary amines from biological fluids and Performance is not affected by drying the sorbent other aqueous matrices Performance is not affected by drying the sorbent EVOLUTE AX 30 um SPE Columns EVOLUTE VVCX 30 um SPE
61. ee EVOLUTE ABN ENV and 101 T Tabless columns 20 21 24 25 30 34 36 42 Technical support 4 Trap Kit VacMaster 59 TPH SPE columns 39 TurboVap 67 Tube Rack TurboVap 67 U Universal PTFE stopcock 57 V VacMaster 10 20 57 VacMaster 96 58 VacMaster accessories 57 59 VacMaster drying adaptor 59 VacMaster LVE kit 59 VacMaster sample processing manifolds 57 59 Vacuum control units VCU 1 58 VCU 2 with integral vacuum generator 58 Vacuum manifold see VacMaster and VacMaster 96 VV VVarranty 74 VVeak anion exchange see NH2 PSA and EVOLUTE VVeak cation exchange see CBA and EVOLUTE 69 Part Number index Part Number Page 121 1010 57 220 0100 P01 25 414001 62 121 1012 57 220 0100 T 25 414007 62 121 1016 57 220 0200 C 25 414008 62 121 1030 57 220 0200 D 25 414141 62 121 1039 57 220 0500 E 25 101 0010 B 24 121 1045 57 220 1000 F 25 101 0020 B 24 121 1132 57 221 0002 A 25 101 0020 C 24 121 1133 57 221 0005 A 25 101 0050 B 24 121 1134 57 221 0005 G 25 101 0050 C 24 121 2010 57 221 0010 A 25 120 1000 P01 46 54 121 2012 57 221 0010 B 25 120 1001 A 53 121 2016 57 221 0010 C 25 120 1002 B 53 121 2059 57 221 0010 G 25 120 1003 C 53 121 2068 57 221 0020 B 25 120 1003 CD 52 121 2075 57 221 0020 C 25 120 1006 D 53 121 2090 59 221 0020 H 25 120 1007 E 53 121 2095 59 221 0025 P01 25 120 1009 F 53 121 2161 57 221 0025 R 25 120 1009 FD 52 121 2162 57 221 0050 B 25 1201 0120 52 121 2163 57 221 0050 BS 25 12
62. filtration column 3 mL 20 um PE ISOLUTE double fritted filtration column 6 mL 20 um PE ISOLUTE double fritted filtration column 10 mL 20 um PE ISOLUTE double fritted filtration column 15 mL 20 um PE ISOLUTE double fritted filtration column 25 mL 20 um PE ISOLUTE double fritted filtration column 70 mL 20 um PE Qty 100 100 100 100 100 100 100 100 Qty 100 100 100 50 100 100 50 Accessories For SPE and Filtration ISOLUTE Sodium Sulfate Drying Cartridges Dry organic solvents with this easy to use format contains 2 5 g of high purity sodium sulfate per cartridge Stack beneath SPE columns during elution for efficient in line solvent drying Part Number Description Qty 802 0250 M TSOLUTE Sodium 50 Sulfate Drying Cartridge 53 Accessorles For SPE and Filtration ISOLUTE Filtration Plates Each well contains a 20 um polyethylene frit for high throughput filtration applications Part Number Description Qty 120 1022 P01 Filtration plate fixed well 1 2 mL 20 um Plate Sealing Accessories Seal wells and Luer outlets of ISOLUTE 96 fixed well plates Piercable sealing cap can also be used to seal collection plates Part Number Description Qty 121 5204 Piercable Sealing Cap 50 121 5205 Luer Cap Mat 25 Deep Well Collection Plates 1 or 2 mL volume per well constructed from high purity solvent resistant polypropylene Seal with piercabl
63. from aqueous sample matrix Analyte elution can be performed by neutralizing charge on the sorbent Sorbent supplied as the free base Alternatively ISOLUTE NH2 can be used for extraction of polar compounds from a non polar matrix using hydrogen bonding retention mechanism Less retentive than SI ISOLUTE NH2 SPE Columns Part Number Description 470 0002 A 470 0005 A 470 0010 A 470 0010 B 470 0010 G 470 0020 B 470 0050 B 470 0050 C 470 0050 H 470 0100 C 470 0200 C 470 0200 D ISOLUTE NH2 25 mg 1 mL ISOLUTE NH2 50 mg 1 mL ISOLUTE NH2 100 mg 1 mL ISOLUTE NH2 100 mg 3 mL ISOLUTE NH2 100 mg 10 mL ISOLUTE NH2 200 mg 3 mL ISOLUTE NH2 500 mg 3 mL ISOLUTE NH2 500 mg 6 mL ISOLUTE NH2 500 mg 10 mL ISOLUTE NH2 1 g 6 mL ISOLUTE NH2 2 g 6 mL ISOLUTE NH2 2 g 15 mL ISOLUTE 96 NH2 Fixed Well Plates Part Number Description 470 0050 1 ISOLUTE 96 NH2 50 mg plate 470 0100 1 TSOLUTE 96 NH2 100 mg plate Support Documents for ISOLUTE NH2 TN102 Method Development in Solid Phase Extraction using Polar Qty 100 100 100 50 50 50 50 30 50 30 30 20 Qty ISOLUTE SPE Columns for the Extraction of Non aqueous Samples TN104 Method Development in Solid Phase Extraction using ISOLUTE NH2 SPE Columns for the Extraction of Aqueous Samples Si O i CH NH CH NH O to the surface of a silica particle Si OH Average particle size 50 um Pore diameter 60 A Sorbent Type VVeak anion
64. g 6 mL Tabless 30 902 0030 H ISOLUTE HCX 300 mg 10 mL 30 902 0050 C ISOLUTE HCX 500 mg 6 mL 30 ISOLUTE 96 HCX Fixed Well Plates Part Number Description Qty 902 0025 P01 ISOLUTE 96 HCX 25 mg plate 1 902 0050 1 ISOLUTE 96 HCX 50 mg plate 1 902 0100 1 ISOLUTE 96 HCX 100 mg plate 1 ISOLUTE Array HCX Loose Wells Part Number Description Qty 902 0025 R ISOLUTE Array HCX 25 mg 1 mL wells 100 902 0050 T ISOLUTE Array HCX 50 mg 2 mL wells 100 902 0100 T ISOLUTE Array HCX 100 mg 2 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE HCX TN116 Generic Method for the Extraction of Basic Drugs from Biological Fluids using ISOLUTE Mixed mode SPE Columns and 96 vvell Plates TN125 Method Development in Solid Phase Extraction using ISOLUTE HCX for the Extraction of Drugs from Biological Fluid Samples ISOLUTE ISOLUTE HCX 3 combines a C18 Octadecyl and OOS HEER SO H sulfonic acid functionalities a N Si O Si CH 17CH N Average particle size 50 um Pore diameter 60 Sorbent Type Mixed Mode Application Extraction of basic analytes from aqueous matrix using dual non polar and strong cation exchange interactions A good alternative to HCX for basic compounds that require more retentive non polar character from the mixed mode sorbent ISOLUTE HCX 3 SPE Columns Part Num
65. hone 46 18 56 5911 E mail eu 1 pointsupport biotage com North amp Latin America Service and Support Telephone 1 800 446 4752 Outside the USA 1 704 654 4900 E mail us 1 pointsupport biotage com Japan Service and Support Telephone 81 3 5627 3123 E mail jp 1 pointsupport biotage com China Service and Support Telephone 86 21 2898 6655 E mail cn 1 pointsupport biotage com To locate a distributor please visit our web site at www biotage com Hovv to Place Your Order In this catalog product information is displayed with easy to follow application details and supporting documentation Ordering information is found following each product listing How to Order You can now order through our web site www biotage com Alternatively you may place your order by telephone email or fax Europe Main Office 46 18 56 59 00 Toll Free 800 18 56 57 10 Fax 46 18 59 19 22 Order Tel 46 18 56 57 10 Order Fax 46 18 56 57 O5 order biotage com North amp Latin America Main Office 1 704 654 4900 Toll Free 1 800 446 4752 Fax 1 704 654 4917 Order Tel 1 704 654 4900 Order Fax 1 434 296 8217 ordermailbox biotage com When placing your order please have available Your purchase order number e Biotage part number s e Product description s e Shipping address e Billing address e Contact person including telephone number e Product username and department Japan Tel 81 3 5627 31
66. id path system with segregated waste lines maintain integrity of sample Positive pressure stepper motor syringe pump delivers precise flow rates Eight solvent reagent lines for maximum methods flexibility L Biotage Magnetically encoded Compatible 1 3 and 6 mL racks of routine samples version SPE columns Part Number Description Part Number Description Qty C50000 RapidTrace Workstation Racks Each RapidTrace requires one rack 1 mL and 3 mL 10 columns C50974 13 x 100 mm Sample Tube 1 C125713 RapidTrace Workstation 12 x 75 Fraction Tube 6 mL 5 columns 3 mL and 1 mL operation possible by purchasing appropriate plunger see accessories brochure C50976 13 x 100 mm Sample Tube 1 12 x 75 Fraction Tube m C58309 13 x 100 mm Sample Tube 1 C52006 RapidTrace Start Up Kit vvith Softvvare 13 x 100 mm Fraction Tube C52689 RapidTrace Notebook Controller C56786 16 x 100 mm Sample Tube 1 16 x 100 mm Fraction Tube C56536 13 x 100 mm Sample Tube 1 RapidTrace Brochure 12 x 75 mm Fraction Tube For further information download the RapidTrace 9 Position Chilled Rack brochure from www biotage com Literature part number C133968 40 mL Sample Flask 5 position 1 RT 10 2011 rev 16 x 100 Fraction Tube 10 position RapidTrace Automated SPE Workstation Biotage 63 64 2015 Analytical Sample Preparation Catalog Biotage 2015 Evaporation Biotage SPE Dry 96 amp 96 Dual Samp
67. ion techniques If you need help choosing the best sample preparation method for your application or want to solve an existing sample preparation problem please contact your local Biotage 1 Point Support team who will ensure you receive fast advice from our sample preparation experts Technical Information Throughout this catalog we highlight chemistry data sheets and application notes that provide detailed information on sample preparation techniques and SPE sorbents Literature Library Biotage s Literature Library is a vital resource for analytical chemists looking to optimize sample preparation procedures As sample preparation experts Biotage are dedicated to finding the ideal solution to your sample preparation requirements with a regularly updated library containing application notes technical notes and scientific presentations The Biotage Literature Library has been optimized to provide the most appropriate efficient and effective sample preparation solutions taking into consideration matrix effects analyte structure functionality and relevant regulatory requirements The Biotage Literature Library is fully searchable by keyword analyte matrix analytical technique format product type and industry type This search feature allows you to obtain relevant application notes suitable for your needs in an efficient and dynamic manner Visit the Biotage Literature Library www biotage com applications ISOLUTE SLE
68. ion using ISOLUTE PSA SPE Columns for the Extraction of Aqueous Samples 33 SOLUTE Columns and Plates ISOLUTE SAX Chemical structure of SAX quaternary amine silane Ai P _ covalently bonded to the DS Di CH Cl surface of a silica particle O SI OH Average particle size 50 pm Pore diameter 60 A Sorbent Type Strong anion exchange Exchange capacity 0 6 meq g Application Extraction of acidic analytes from aqueous sample matrix Supplied vvith chloride counter ion ISOLUTE SAX SPE Columns ISOLUTE PE AX Chemical structure of PE AX quaternary amine Si 0 5i CH N CH Silane covalently bonded O to the surface of a silica CH COO particle pi On Average particle size 50 um Pore diameter 60 A Sorbent Type Strong anion exchange Exchange capacity 0 6 meq g Application Extraction of acidic analytes from aqueous sample matrix Supplied vvith acetate counter ion for more efficient extraction of acidic analytes including those vvith polar vvater soluble characteristics ISOLUTE PE AX SPE Columns Part Number Description Qty 503 0002 A ISOLUTE PE AX 25 mg 1 mL 100 503 0005 A ISOLUTE PE AX 50 mg 1 mL 100 503 0010 A ISOLUTE PE AX 100 mg 1 mL 100 503 0010 B ISOLUTE PE AX 100 mg 3 mL 50 503 0010 C ISOLUTE PE AX 100 mg 6 mL 30 503 0020 B ISOLUTE PE AX 200 mg 3 mL 50 503 0050 B ISOLUTE PE AX 500 mg 3 mL 50 503 0050 C ISOLUTE PE AX 500 mg 6 mL 30 503 0100 C ISOLUTE PE AX 1 g 6 mL 30 503 020
69. l structure of C2 Silane covalently bonded to the surface of a silica 51 0 51 CH CH particle x 9 Average particle size 50 um Pore diameter 60 A Sorbent Type Non Polar Application Most commonly used C2 sorbent for the extraction of acidic neutral and basic compounds from aqueous matrices Secondary silanol or ionic interactions can be used to enhance extract purity and method robustness for basic compounds ISOLUTE C2 SPE Columns Part Number Description Qty 320 0002 A ISOLUTE C2 25 mg 1 mL 100 320 0005 A ISOLUTE C2 50 mg 1 mL 100 320 0010 A ISOLUTE C2 100 mg 1 mL 100 320 0010 B ISOLUTE C2 100 mg 3 mL 50 320 0010 G ISOLUTE C2 100 mg 10 mL 50 320 0020 B ISOLUTE C2 200 mg 3 mL 50 320 0050 B ISOLUTE C2 500 mg 3 mL 50 320 0050 C ISOLUTE C2 500 mg 6 mL 30 320 0100 C ISOLUTE C2 1 g 6 mL 30 320 0200 D ISOLUTE C2 2 g 15 mL 20 ISOLUTE 96 C2 Fixed Well Plates Part Number Description Qty 320 0025 P01 ISOLUTE 96 C2 25 mg plate 1 320 0050 1 ISOLUTE 96 C2 50 mg plate 1 ISOLUTE Array C2 Loose Wells Part Number Description Qty 320 0025 R ISOLUTE Array C2 25 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE C2 TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic
70. le Concentrator System Biotage SPE Dry 96 Sample Concentrator System Part Number Description SD 9600 DHS NA SPE Dry 96 Sample Concentrator System 100 120V SPE Dry 96 Dual Biotage SD 9600 DHS EU SPE Dry 96 Sample Concentrator System 220 240V SD 9600 DHS T NA SPE Dry 96 Sample Concentrator System with PTFE coated needles Top Head Only 100 120V SD 9600 DHS T EU SPE Dry 96 Sample Concentrator System with PTFE coated needles Top Head Only 220 240V Biotage SPE Dry 96 Dry Dual Sample Concentrator System Part Number Description SD2 9600 DHS NA SPE Dry 96 Dual Sample Concentrator System 100 120V SD2 9600 DHS EU SPE Dry 96 Dual Sample Concentrator System 220 240V SD2 9600 DHS T NA SPE Dry 96 Dual Sample Concentrator System with PTFE coated needles Biotage SPE Dry 96 Dual Top Head Only 100 120V SD2 9600 DHS T EU SPE Dry 96 Dual Sample Concentrator System with PTFE coated needles Top Head Only 220 240V Evaporation Brochure For further information download the Evaporation brochure from www biotage com Literature part number PPS296 Solvent Evaporation Fast Reliable and Affordable x Biotage 2015 Analytical Sample Preparation Catalog Biotage 2015 TurboVap Evaporators and Concentrators TurboVap Product Family TurboVap 500 TurboVap 96 TurboVap II TurboVap LV Part Number Description TurboVap 500 C103202 TurboVap 500 100 120V 50 60Hz C103203 Turb
71. le Preparation Catalog Biotage 2015 Sample Preparation Accessories Accessorles For SPE and Filtration 120 1103 PE Adaptor 70 mL column F 120 1104 PTFE Adaptor 6 mL Glass columns amp ISOLUTE Column Adaptors ISOLUTE Depth Filter Reservoirs Column adaptors attach empty reservoirs filtration Reservoirs with pre fitted depth filters remove particulate columns and depth filters to SPE columns matter from aqueous samples and prevent column blockage Also allow for separate analysis of particulate Part Number Description Qty bound compounds 120 1100 PTFE Adaptor 1 3 amp 6 mL and XL 10 columns A B C G amp H Part Number Description Qty 120 1101 PE Adaptor 1 3 amp 6 mL 10 120 1003 CD ISOLUTE depth g 100 columns A B amp C filter 6 mL 120 1102 PE Adaptor 15 amp 25 mL 10 120 1009 FD ISOLUTE depth 50 columns D amp E filter 70 mL ag 4 1 2 ISOLUTE Column Caps Seal prepared columns sample loaded columns or immunoaffinity columns for transportation or storage Part Number Description Column Qty Type 1201 0120 Bottom Luer cap All 100 fits all columns 1201 0121 A Top cap 1 mL column A 100 1201 0122 B Top cap 3 mL column B 100 1201 0123 C Top cap 6 mL column C 100 1201 0125 H Top cap 10 mL G amp H 100 XL column 1201 0126 D Top cap 15 mL column D 100 1201 0127 E Top cap 25 mL column E 100 1201 0128 F Top cap 70 mL column F 100 2015 Ana
72. lytical Sample Preparation Catalog Biotage 2015 ISOLUTE Empty Reservoirs Stack above SPE columns to increase the reservoir volume Part Number Description 120 1001 A ISOLUTE Reservoir 1 mL 120 1002 B ISOLUTE Reservoir 3 mL 120 1003 C ISOLUTE Reservoir 6 mL 120 1006 D ISOLUTE Reservoir 15 mL 120 1007 E ISOLUTE Reservoir 25 mL 120 1009 F ISOLUTE Reservoir 70 mL ISOLUTE Frits Qty 100 100 100 100 100 50 20 um porosity sintered polyethylene frits to fit all SPE column sizes Part Number Description Column Type 120 1031 A ISOLUTE Frit 6 mm A 1 mL 1 4 diameter 20 um PE 120 1032 G ISOLUTE Frit 6 mm G XL 1 4 diameter 20 um PE 120 1033 B ISOLUTE Frit 9 mm B 3 mL 120 1034 H 120 1035 C 120 1036 D 120 1037 E 120 1038 F 7 8 diameter 20 um PE ISOLUTE Reservoir 15 mL H XL 7 57 diameter 20 um PE SOLUTE Frit 13 mm 1 27 C 6 mL diameter 20 um PE ISOLUTE Frit 16 mm s D 15 mL diameter 20 um PE ISOLUTE Frit 20 mm E 25 mL 57 67 diameter 20 um PE ISOLUTE Frit 27 mm F 70 mL 11 16 diameter 20 um PE ISOLUTE Filtration Columns Pre fitted with two 20 um polyethylene frits Use as standalone filters or stacked above an SPE column Part Number Description 120 1021 A 120 1022 6 120 102 120 1024 H 120 1025 D 120 1026 E 120 1028 F ISOLUTE double fritted filtration column 1 mL 20 um PE ISOLUTE double fritted
73. material may be reproduced or published without the written permission of Biotage Information in this document is subfect to change vvithout notice and does not represent any commitment from Biotage ESOE A list of all trademarks owned by Biotage AB is available at www biotage com legal Other product and company names mentioned herein may be trademarks or registered trademarks and or service marks of their respective owners and are used only for explanation B 1 d e and to the owners benefit without intent to infringe
74. mg 1 mL 100 915 0005 B ISOLUTE ENV 50 mg 3 mL 50 915 0005 G ISOLUTE ENV 50 mg 10 mL 50 915 0010 A ISOLUTE ENV 100 mg 1 mL 100 915 0010 B ISOLUTE ENV 100 mg 3 mL 50 915 0010 BG ISOLUTE ENV 100 mg 3 mL Tabless 50 915 0010 C ISOLUTE ENV 100 mg 6 mL 30 915 0010 H ISOLUTE ENV 100 mg 10 mL 50 915 0020 B ISOLUTE ENV 200 mg 3 mL 50 915 0020 C ISOLUTE ENV 200 mg 6 mL 30 915 0020 CD ISOLUTE ENV 200 mg 6 mL Depth filter 30 915 0020 CG ISOLUTE ENV 200 mg 6 mL Tabless 30 915 0050 B ISOLUTE ENV 500 mg 3 mL 50 915 0050 BG ISOLUTE ENV 500 mg 3 mL Tabless 50 Chemical structure ISOLUTE 101 of ISOLUTE 101 an Src ene unmodified polystyrene ace divinylbenzene co polymer ig ar x Average particle size 50 um Pore diameter 100 A Sorbent Type Non Polar Application Extraction of polar compounds from aqueous sample matrices ISOLUTE 101 SPE Columns Part Number Description Qty 101 0010 B ISOLUTE 101 100 mg 3 mL 50 101 0020 B ISOLUTE 101 200 mg 3 mL 50 101 0020 C ISOLUTE 101 200 mg 6 mL 30 101 0050 B ISOLUTE 101 500 mg 3 mL 50 101 0050 C ISOLUTE 101 500 mg 6 mL 30 Support Documents for ISOLUTE 101 TN119 Method Development in Solid Phase Extraction using ISOLUTE 101 SPE Columns for the Extraction of Aqueous Samples 915 0050 C ISOLUTE ENV 500 mg 6 mL 30 915 0050 D ISOLUTE ENV 500 mg 15 mL 20 915 0050 L ISOLUTE ENV 500 mg 6 mL Glass 30 915 0100 C ISOLUTE ENV 1 g 6 mL 30 915 0100 E ISOLUTE EN
75. moved from plasma and other blood based samples while high reproducible analyte recoveries are maintained The optimized frit arrangement acts as a depth filter efficiently trapping precipitated proteins without blocking or plugging ISOLUTE PLD plates can be processed using positive pressure or vacuum processing systems see page 56 for ordering information 18 Step 2 Dispense sample matrix e g plasma blood with force Iso S0 p 332535 01 l Unu Biotage lz Step 3 Apply low vacuum positive pressure Mix if required Precipitated proteins scat yi dr retained by depth filter 99 l Phospholipids retained v v 4 Collect purified analytes A A V 4 gt Part Number Description Qty 918 0050 1 ISOLUTE PLD Protein and Phospholipid 1 Removal Plate Accessories Part Number Description Qty 121 5202 Collection plate 1 mL 50 121 5203 Collection plate 2 mL 50 For more information visit www biotage com 2015 Analytical Sample Preparation Catalog Biotage 2015 EVOLUTE Sample Preparation Products Advanced Polymeric Sorbents 20 EVOLUTE Advanced Polymeric Sorbents EVOLUTE ABN Polar hydrophilic groups Non polar hydrophobic groups Chemical structure of EVOLUTE ABN Chemical Description Water wettable polystyrene divinylbenzene incorporating non ionizable hydroxyl groups Average particle size 30 um 50 um Pore diameter 40 A S
76. n exchange with the positively charged surface or by specific interactions with the metal center ISOLUTE AL A has a nominal moisture content of lt 0 1 Brockman Activity Super 1 grade and surface pH of 4 5 ISOLUTE AL N Neutral surface allows interactions of the aluminum metal center with compounds whose heteroatoms are electronegative e g N O P amp S or whose highly aromatic structure makes them electron rich The adsorbent can be useful for retaining amines and aromatic compounds from either aqueous or non aqueous solvents ISOLUTE AL N has a nominal moisture content of lt 0 1 Brockman Activity Super 1 grade and surface pH of 7 5 ISOLUTE AL B Washing this material with a basic solution results in a net negative charge Cationic compounds are retained on the negatively charged surface or by specific interactions with the metal center ISOLUTE AL B has a nominal moisture content of lt 0 1 Brockman Activity Super 1 grade and surface pH of 10 Column Qty Part Numbers configuration AL A AL N AL B 100 mg i mL 100 713 0010 A 714 0010 A 715 0010 A 200 mg 3 mL 50 713 0020 B 714 0020 B 715 0020 B 500 mg 3 mL 50 713 0050 B 714 0050 B 715 0050 B 500 mg 6 mL 30 713 0050 C 714 0050 C 715 0050 C 1 g 3 mL 50 713 0100 B 714 0100 B 715 0100 B 1 g 6 mL 30 713 0100 C 714 0100 C 715 0100 C 2 g 6 mL 30 714 0200 C uni 2 15 mL 20 713 0200 D 714 0200 D 715 0200 D 5 g 25 mL 20 713 0500 E 714 0500 E 715 0500 E 1
77. nt 44 Packed columns 38 ISOLUTE Myco SPE columns 41 ISOLUTE PLD 17 ISOLUTE QuEChERS 47 ISOLUTE SLE 4 13 ISOLUTE SPE columns see individual sorbent chemistries For example for ISOLUTE C18 see C18 L Large volume extraction LVE kits 59 Layered columns 39 Literature Library 4 M Manual sample processing products and accessories 55 60 Method development assistance 4 MFC18 n Octadecyl monofunctional 96 well SPE plates 26 SPE columns 26 Mixed mode SPE columns 30 32 Molecularly Imprinted Polymers 49 Multimode 32 2015 Analytical Sample Preparation Catalog Biotage 2015 Needles PTFE and stainless steel 57 NH2 Aminopropyl 96 vvell SPE plates 33 Bulk sorbent 44 SPE columns 33 Non polar sorbents 4 4 Non polar ISOLUTE SPE columns and 96 well SPE plates 24 29 O Octadecyl see C18 C18 EC MFC18 Octyl see C8 C8 EC Oil amp Grease column O amp G 40 Ordering How to Place Your Order 6 P Part numbering system 10 Part Number Index 70 PAH SPE columns 39 PE AX Pre equilibrated anion exchanger 96 well SPE plates 34 SPE columns 34 PH Phenyl 96 well SPE plates 29 SPE columns 29 Phenyl see PH Plate sealing accessories 54 Polar sorbents 44 Polyethylene frits 53 Polypropylene reservoirs 53 Polystyrene divinylbenzene see EVOLUTE ABN 101 and ENV Primary secondary amine see PSA PRESSURE 48 and PRESSURE 96 Positive Pressure Manifolds 56 Protein and Phospholipid Removal
78. o LC MS MS Analysis AN784 Extraction of Multiple Mycotoxins From Nuts Using ISOLUTE Myco prior to LC MS MS Analysis AN785 Extraction of Aflatoxins and Ochratoxin from Dried Chili Using ISOLUTE Myco prior to LC MS MS ANS804 Extraction of Multiple Mycotoxins From Animal Feed Using ISOLUTE Myco SPE Columns prior to LC MS MS Analysis AN807 Extraction of Aflatoxin M1 From Infant Formula Using ISOLUTE Myco SPE Columns prior to LC MS MS Analysis AN823 Extraction of Multiple Mycotoxins From Cereal Based Infant Food Using ISOLUTE Myco prior to UHPLC MS MS Analysis ISOLUTE Myco Columns Part Number Description 150 0006 BG ISOLUTE 60 mg 3 mL Tabless Extraction of Multiple Mycotoxins From Grain s Using ISOLUTE Myco prior to LC MS MS Analysis scribe thigh This application note describes a Solid Phase Extraction SPE protocol for the extraction of a range of mycotoxins ange of rain ment from wheat flour wheat maize and barley using ISOLUTE Myco with LC MS MS a high y pean Introduction he b Mytotoxins t metabolites p fungal mol wot af ses from food crops Regulat d legisiat for testing of m contamination has established which mycotoxins are preva lent on a wide ps This application ane Vu trom ets priste for LC MS MS analysis 1 SIMS In n food Crops alang Hits can 6 SIMS rain tes w s Quire OH O H m European regulatie ent of these o
79. oVap 500 220 240V 50 60Hz TurboVap 96 C103263 TurboVap 96 100 120V C103264 TurboVap 96 220 240V TurboVap II C103186 TurboVap II 200 mL with 0 5 mL Endpoint Stem 100 120V C103187 TurboVap II 200 mL with 1 0 mL Endpoint Stem 100 120V C103188 TurboVap II 50 mL with 0 5 mL Endpoint Stem 100 120V C103189 TurboVap II 50 mL with 1 0 mL Endpoint Stem 100 120V C103190 TurboVap II 200 mL with 0 5 mL Endpoint Stem 220 240V C103192 TurboVap II 200 mL with 1 0 mL Endpoint Stem 220 240V C103195 TurboVap II 50 mL with 0 5 mL Endpoint Stem 220 240V C103194 TurboVap II 50 mL with 1 0 mL Endpoint Stem 220 240V C103196 TurboVap II 40 mL Dionex ASE Vial Compatible 100 120V C103197 TurboVap II 40 mL Dionex ASE Vial Compatible 220 240V TurboVap LV C103198 TurboVap LV without Racks 100 120V C103199 TurboVap LV without Racks 220 240V C103200 TurboVap LV ASE 40 60 mL vial 100 120V C103201 TurboVap LV ASE 40 60 mL vial 220 240V C112175 TurboVap LV PTFE 50 position 100 120V C133718 TurboVap LV PTFE 50 position 220 240V C55941 TurboVap LV PTFE Coated Upper Manifold Replacement Kit C109301 TurboVap LV Assy Water Bath PTFE Coated Tube Racks C48950 Tube Rack for 10 x 75 mm tubes P N C48985 C44577 Tube Rack for 12 x 75 mm tubes P N C44651 C45286 Tube Rack for 13 x 100 mm tubes P N C40707 C44139 Tube Rack for 16 x 100 mm tubes P N C40708 C45269 Tube Rack for 16 x 125 mm tubes P N C45273 C44282 Tube Rack for
80. oice when working at extreme pH C18 is the preferred option for these methods ISOLUTE MFC18 SPE Columns Part Number Description Qty 240 0005 A ISOLUTE MFC18 50 mg 1 mL 100 240 0005 G ISOLUTE MFC18 50 mg 10 mL 50 240 0010 A ISOLUTE MFC18 100 mg 1 mL 100 240 0010 B ISOLUTE MFC18 100 mg 3 mL 50 240 0010 G ISOLUTE MFC18 100 mg 10 mL 50 240 0020 B ISOLUTE MFC18 200 mg 3 mL 50 240 0020 H ISOLUTE MFC18 200 mg 10 mL 50 240 0050 B ISOLUTE MFC18 500 mg 3 mL 50 240 0050 C ISOLUTE MFC18 500 mg 6 mL 30 240 0050 H ISOLUTE MFC18 500 mg 10 mL 50 240 0100 C ISOLUTE MFC18 1 g 6 mL 30 240 0200 D ISOLUTE MFC18 2 g 15 mL 20 ISOLUTE 96 MFC18 Fixed Well Plates Part Number Description Qty 240 0025 1 ISOLUTE 96 MFC18 25 mg plate 1 Support Documents for ISOLUTE MFC18 TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents ISOLUTE C8 Chemical structure of C8 Silane covalently bonded to the surface of a silica LL Si O Si CH CH particle o Si OH Average particle size 50 um Pore diameter 60 A Sorbent Type Non Polar Application Most commonly used C8 sorbent for the extraction of acidic neutral and basic compounds from aqueous matrices Secondary silanol or ionic interactions can be used to enhance extract purity
81. ollection plate waste plate 96 column sealing gasket gas supply adaptor kit 6 of 1 8 i d polyethylene tubing and 1 8 and 1 4 NPT connectors User Manual CD ROM PRESSURE 96 Sealing Gasket 96 position PRESSURE 96 Collection Tray 10 mL 24 well PPM GA PRESSURE Gas Supply Adaptor for all models 121 5202 96 well collection plate 1 mL pack of 50 121 5203 96 well collection plate 2 mL pack of 50 H Biotage PRESSURE 48 Part Number PPM 48 Racks PPM A48 1RCK PPM A48 3RCK PPM A48 6RCK PPM A48 1275 PPM A48 13100 PPM A48 1232 PPM A48 16100 Accessories PPM A48 GSKT PPM A48 WST PPM GA Description PRESSURE 48 Positive Pressure Manifold 48 position Includes Sealing gasket 48 position gas supply adaptor kit 6 of 1 8 i d polyethyl ene tubing and 1 8 and 1 4 NPT connectors waste bin rack waste bin inserts User Manual CD ROM Racks must be ordered separately PRESSURE 48 SPE Column Rack 1 mL PRESSURE 48 SPE Column Rack 3 mL PRESSURE 48 SPE Column Rack 6 mL PRESSURE 48 Collection Rack 12 X 75 mm PRESSURE 48 Collection Rack 13 x 100 mm PRESSURE 48 Sample Vial Rack 12 x 32 mm PRESSURE 48 Collection Rack 16 x 100 mm PRESSURE 48 Sealing Gasket 48 position PRESSURE 48 Waste Bin Inserts PRESSURE Gas Supply Adaptor for all models 2015 Analytical Sample Preparation Catalog Biotage 2015 Biotage VacMaster 10 and 20 10 or 20 position vacuum manifolds for SPE
82. onstant e Consistent column to column and batch to batch recoveries Well controlled particle size distribution with minimal fines from column to column and batch to batch e Reliable sample processing using manifolds and automated devices Consistent flow through columns from column to column and batch to batch Low back pressure automation friendly Gravity loading of samples with some column configurations No fines in final extract to plug injectors or absorb analytes when sample is reconstituted in another solvent No channeling in sorbent beds minimizes sorbent mass requirements reducing elution volumes and costs Minimal drying time reproducible from batch to batch e Large sorbent mass columns with good flow characteristics 4 Quality Assurance Report Biotage Part No 221 0010 A Descriptio ISOLUTE C18 EC 100mg imi SPE Columns Lot No 10221002GP Sorbent Batch No 221 0 02 This product has been manufactured tested and assembled under the control of an ISO 9001 2008 registered quality system and has been subjected to the following QC tests Base Material Analysis Material HPLC Analvsis Pore Diameter A Mobile Phase _60 MeOH 40 H20 Surface Area m g Uracil Surface pH Acetophenone robenzene Methyl Benzoate Carbon Loading C 5 Toluene Bonded Phase Coverage umol m 1 6 Capacity Factor K PASS UV Abs of MeOH Extract 254 nm 0 1 Selectivity Alpha 4 3 PASS Extraction Residue
83. orbent Type VVettable non polar sorbent with no secondary interactions Application EVOLUTE ABN can be used to extract a diverse range of acidic neutral and basic analytes from biological fluids and other aqueous matrices Performance is not affected by drying the sorbent EVOLUTE ABN 30 um SPE Columns Part Number Description Qty 600 0001 A EVOLUTE ABN 10 mg 1 mL 100 600 0002 A EVOLUTE ABN 25 mg 1 mL 100 600 0002 AG EVOLUTE ABN 25 mg 1 mL Tabless 100 600 0002 B EVOLUTE ABN 25 mg 3 mL 50 600 0002 H EVOLUTE ABN 25 mg 10 mL XL 50 EVOLUTE ABN 50 um SPE Columns 610 0005 B EVOLUTE ABN 50 mg 3 mL 50 610 0005 BG EVOLUTE ABN 50 mg 3 mL Tabless 50 610 0010 B EVOLUTE ABN 100 mg 3 mL 50 610 0010 H EVOLUTE ABN 100 mg 10 mL XL 50 610 0020 B EVOLUTE ABN 200 mg 3 mL 50 610 0020 C EVOLUTE ABN 200 mg 6 mL 30 610 0050 C EVOLUTE ABN 500 mg 6 mL 30 EVOLUTE EXPRESS ABN 30 um Fixed Well Plates 600 0010 PX01 EVOLUTE EXPRESS ABN 1 10 mg Fixed Well Plate 600 0030 PX01 EVOLUTE EXPRESS ABN 1 30 mg Fixed Well Plate EVOLUTE ABN 30 um Pre Assembled 96 Well Plates 600 0010 RP EVOLUTE Array ABN 10 mg 1 mL Plate 1 600 0025 RP EVOLUTE Array ABN 25 mg 1 mL Plate 1 EVOLUTE ABN 30 um Array Loose Wells 600 0010 R EVOLUTE Array ABN 10 mg Wells 100 600 0025 R EVOLUTE Array ABN 25 mg Wells 100 EVOLUTE CX All pH conditions Chemical structure of EVOLUTE CX Chemical Description Sulfonic acid modified polystyrene divinylbenzene incorporating
84. pply solvent Plasma occurs no vacuum vortex mixing required Protein is retained by depth filter system Optimized filtration aes Vacuum system ae Collect purified Bottom frit prevents dripping filtrate Protein precipitation procedure using ISOLUTE PPT plates 2015 Analytical Sample Preparation Catalog Biotage 2015 ISOLUTE QuEChERS Fast and Efficient Clean up of Complex Food Samples Biotage 2015 47 SOLUTE QuEChERS ISOLUTE QuEChERS ISOLUTE QuEChERS Quick Easy Cheap Effective Rugged and Safe products provide simple clean up of complex samples using salt assisted extraction and partitioning followed by dispersive SPE The pre weighed extraction and clean up tubes conform to AOAC and EN methodologies and include options for waxed and highly pigmented fruit and vegetable samples LUTE QUEChERS EN FRUIT VEGE TABLE ISOLUTE QuECHERS VVAXED f RUIT VEG AUDAU Extraction Tubes Part Number Description Tube Pack Size MgSO Na Na Na Citrate NaCl Acetate Citrate sesqui hydrate 15 g QuEChERS AOAC LLL 15 mL Extraction Tube ne og 10 g QuEChERS EN Clean up Tubes Part Number Description Tube Pack Size PSA MgSO 18 GCB purest AOAC Fruit and Vegetables Q0030 15V Clean up Tube 15 mL 25 400 mg 1200 mg EN Fruit and Vegetable Q0035 15V Clean up Tube 15 mL 25 150 mg 900 mg AOAC Waxed Fruit and Vegetables 15V 1 L 2 4 12 4 Q0050 15 Clean p Tub
85. right 11 12 2015 Analytical Sample Preparation Catalog Biotage 2015 ISOLUTE SLE Supported Liquld Extraction Columns and Plates Simple Load Wait Elute Methodology 14 Supported Liquid Extraction Achieving Simplicity and Success in Sample Preparation ISOLUTE SLE Plates and Columns for Supported Liquid Extraction Improve productivity and maximize analyte recovery using a simple Load Wait Elute methodology Step 1 Load A Analyte A Matrix components e g phospholipids salts and proteins SLE Support material diatoms Aqueous sample flows onto extraction bed and is dispersed in small droplets Supported liquid extraction mechanism The recommended workflow for processing ISOLUTE SLE columns and plates Pre treat sample as required Ensure appropriate collection vessel is in place Load sample onto ISOLUTE SLE column or plate RB U N e Apply vacuum 15 Hg 0 5 bar or pressure 3 5 psi for 5 seconds to initiate loading 5 Wait 5 minutes for sample to completely absorb and form extraction layer 6 Apply water immiscible extraction solvent and allow to flow for 5 minutes under gravity 7 Apply vacuum 15 Hg 0 5 bar or pressure 10 15 psi for 20 30 seconds to complete elution 8 Evaporate eluate to dryness and reconstitute as required Chemical Description Modified form of diatomaceous earth Step 3 Elute Analytes partition into elution solvent and
86. rray SAX 50 mg 1 mL wells 100 500 0100 T ISOLUTE Array SAX 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents for ISOLUTE SAX TN103 Method Development in Solid Phase Extraction using ISOLUTE PE AX and SAX SPE Columns for the Extraction of Aqueous Samples PE AX and SAX SPE Columns for the Extraction of Aqueous Samples Tabless columns for use with PRESSURE Positive Pressure Manifolds and other automated SPE systems Other tabless columns are available contact Biotage for details 2015 Analytical Sample Preparation Catalog Biotage 2015 ISOLUTE CBA Chemical structure of CBA Silane covalently bonded to the surface of a silica particle SI OH 2 Average particle size 50 um Pore diameter 60 A Sorbent Type VVeak cation exchange pka 4 8 or polar Exchange capacity 0 6 meq g Application Extraction of strong bases and polybasic compounds from aqueous sample matrix Analyte elution can be performed by neutralizing charge on the sorbent ISOLUTE CBA SPE Columns Part Number Description Qty ISOLUTE SCX No SO H pl OF Average particle size 50 um Pore diameter 60 A Sorbent Type Exchange capacity 0 4 meq g SOLUTE Columns and Plates Chemical structure of benzenesulfonic acid functional group covalently bonded to the surface of a Silica particle Strong c
87. s for Mycotoxin Analysis 42 ISOLUTE Myco ISOLUTE Myco SPE columns offer simple and efficient multiple mycotoxin sample preparation from a wide range of matrices ideally suited for selective and fast LC MS MS analysis ISOLUTE Myco SPE columns contain a novel polymer based sorbent designed specifically to be selective enough to isolate a wide variety of different mycotoxins ISOLUTE Myco SPE columns are available in tabless flangeless format for use on both manual and automated sample processing manifolds and systems Matrix specific application notes Each method confidently meets the maximum residue limits MRL set down by EU and US regulations in terms of detection limits RSD and recoveries in most cases far exceeding these requirements ISOLUTE Myco clean up methods are quick simple to use and require no offline steps making the technique suitable for automation especially useful for high throughput testing laboratories Unlike immunoaffinity columns ISOLUTE Myco columns are cost effective easy to use and have no special storage requirements Matrix Specific Application Notes Matrix specific application notes now available AN781 Extraction of Patulin from Apple Juice Using ISOLUTE Myco prior to LC MS MS Analysis AN782 Extraction of Multiple Mycotoxins From Grain Using ISOLUTE Myco prior to LC MS MS Analysis AN783 Extraction of Deoxynivalenol From Grain Using ISOLUTE Myco prior t
88. taneously Part Number Description Qty 124 1001 VacMaster 10 Drying Adaptor 1 for 1 3 amp 6 mL columns 124 2001 VacMaster 20 Drying Adaptor 1 for 1 3 amp 6 mL columns Biotage VacMaster Trap Kit VVaste traps should be installed betvveen the outlet ofthe VacMaster sample processing manifold and the vacuum source trapping all vvaste liquids exiting the manifold Compatible with VacMaster 10 amp 20 and 96 processing manifolds VacMaster Trap Kits are available with 1 L or 10 L capacity Part Number Description Qty 121 2095 VacMaster Trap Kit 1 L 1 121 2195 VacMaster Trap Kit 10 L 1 H TM Biotage VacMaster Large Volume Extraction LVE Kit For unattended loading of large volume samples Inert PTFE tubing prevents sample contamination Part Number Description Qty 121 2090 VacMaster LVE kit complete 1 for 1 3 and 6 mL columns 59 60 Biotage Gravity Rack Biotage Gravity Rack Process up to 20 samples simultaneously under gravity Part Number Description Qty 123 2016 Gravity Rack with 16 mm 1 collection tube rack 123 2019 Gravity Rack with 19 mm 1 collection tube rack Accessories Part Number Description Qty 121 0009 Universal PTFE stopcock 10 121 0001 PTFE stopcock needle unit 10 121 0002 PTFE needle unit 10 121 0003 Stainless steel needle 20 121 0004 Stainless steel needle retainer 10 2015 Analytical Sample Preparation Catalog Biotage 2015 Automated Sample
89. ts for ISOLUTE CN TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN102 Method Development in Solid Phase Extraction using Polar ISOLUTE SPE Columns for the Extraction of Non aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents 29 30 SOLUTE Columns and Plates ISOLUTE HCX ISOLUTE HCX combines C8 Octyl and sulfonic acid functionalities N Si O Si R SO H Si O i CH CH Average particle size 50 pm Pore diameter 60 A Sorbent Type Mixed Mode Application The first choice sorbent for extracting drugs of abuse from biological fluid samples ISOLUTE HCX SPE Columns Part Number Description Qty 902 0002 A ISOLUTE HCX 25 mg 1 mL 100 902 0002 AG ISOLUTE HCX 25 mg 1 mL Tabless 100 902 0005 A ISOLUTE HCX 50 mg 1 mL 100 902 0010 A ISOLUTE HCX 100 mg 1 mL 100 902 0010 B ISOLUTE HCX 100 mg 3 mL 50 902 0013 A ISOLUTE HCX 130 mg 1 mL 100 902 0013 B ISOLUTE HCX 130 mg 3 mL 50 902 0013 BG ISOLUTE HCX 130 mg 3 mL Tabless 50 902 0013 C ISOLUTE HCX 130 mg 6 mL 30 902 0013 CG ISOLUTE HCX 130 mg 6 mL Tabless 30 902 0013 H ISOLUTE HCX 130 mg 10 mL 50 902 0020 B ISOLUTE HCX 200 mg 3 mL 50 902 0020 H ISOLUTE HCX 200 mg 10 mL 50 902 0030 B ISOLUTE HCX 300 mg 3 mL 50 902 0030 C ISOLUTE HCX 300 mg 6 mL 30 902 0030 CG ISOLUTE HCX 300 m
90. velopment in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples 2015 Analytical Sample Preparation Catalog Biotage 2015 ISOLUTE PH Chemical structure of phenyl silane covalently b i bonded to the surface of SI OT SI a silica particle 2 Si OH Average particle size 50 um Pore diameter 60 Sorbent Type Non Polar Application Extraction from aqueous matrix using both non polar interactions for acidic neutral and basic compounds and secondary silanol or ionic interactions for basic compounds only This sorbent exhibits a different selectivity compared with C18 and C8 phases when both aromatic and non aromatic compounds are being extracted ISOLUTE PH SPE Columns Part Number Description Qty 360 0002 A ISOLUTE PH 25 mg 1 mL 100 360 0005 A ISOLUTE PH 50 mg 1 mL 100 360 0010 A ISOLUTE PH 100 mg 1 mL 100 360 0010 B ISOLUTE PH 100 mg 3 mL 50 360 0050 B ISOLUTE PH 500 mg 3 mL 50 360 0050 C ISOLUTE PH 500 mg 6 mL 30 360 0100 C ISOLUTE PH 1 g 6 mL 30 ISOLUTE 96 PH Fixed Well Plates Part Number Description Qty 360 0025 1 ISOLUTE 96 PH 25 mg plate 1 360 0050 P01 ISOLUTE 96 PH 50 mg plate 1 360 0100 1 ISOLUTE 96 PH 100 mg plate 1 ISOLUTE Array PH Loose Wells Part Number Description Qty 360 0100 T ISOLUTE Array PH 100 mg 2 mL wells 100 Pre assembled 1SOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number
91. xtraction Solvent 4 MTBE DCM DCM IPA 95 5 v v Ethyl Acetate 96 Well Plates Array Wells Part Number Description Qty Part Number Description Qty 820 0200 P01 ISOLUTE SLE 200 uL 1 820 0200 T ISOLUTE SLE 200 HL Array Wells 100 Supported Liquid Extraction Plate 820 0400 T ISOLUTE SLE 400 uL Array Wells 100 820 0400 P01 ISOLUTE SLE 400 uL 1 Supported Liquid Extraction Plate 48 Well Plates Extract 1 mL sample volumes in high throughput microplate format Accessories Part Number Description Qty Part Number Description Qty 820 1000 Q01 ISOLUTE SLE 1 mL Supported Liquid 1 121 5202 Collection plate 1 mL 50 Extraction Plate 48 well 121 5203 Collection plate 2 mL 50 121 5210 Collection plate 5 mL 48 well 20 Columns Part Number Description Qty 820 0055 B ISOLUTE SLE 400 uL Sample Volume 50 pe 2 820 0055 BG ISOLUTE SLE 400 uL Sample Volume 50 ISOLUTE SLE User Guide 775 For further information download the ISOLUTE SLE U 820 0140 C ISOLUTE SLE 1 mL Sample Volume 30 820 0140 CG ISOLUTE SLE 1 mL Sample Volume 30 Guide from www biotage com Literature part number Tabless Ul304 V 2 820 0290 D ISOLUTE SLE 2 mL Sample Volume 20 820 0690 E ISOLUTE SLE 5 mL Sample Volume 20 820 1420 F ISOLUTE SLE 10 mL Sample Volume 16 ISOLUTE SLE User Guide Bulk Packs To reduce packaging and improve workflow ISOLUTE SLE columns are available in bulk pack sizes Part Number Description Qty 820 0055 B 500 ISOLUTE SLE 400

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