Flexcell® FX-5000™ Compression System
Contents
1. E eee eker kek ee keke kek ke ke RA AAS A RA DEA Ke 15 3 11 Res ming Regimes aca kan e le wn ea ie vc xurek n n Mak Aida b ra n k EW a ewan nea 15 E FLEXCELL INTERNATIONAL CORPORATION 3 12 Stopping Regimens AE eee eke kek e kek ke ke ke kek Ke keke KRA KE KK KA KAK Ke 15 3 13 Determine the Progress of the Regimen keke kek kek k 15 3 14 Resetting the FlexLink eee rent eet eat eee oaeesoeaooaseusoosoesoensoaseusooun 16 3 15 Using the Pressure Force Conversion Calculator E e kee 16 3 16 Data AnalySiS es ka la e at e a d a en an tina saute j Se eae aud erm 16 3 17 Exiting the FX 5000 Program c cceccsccssssssssssessscsssssesesssssessessessessesnesessseeesessees 17 4 System Troubleshooting foi e pa Ske ke bi ka a b ie 18 5 Application IN OU Si t k fey ik kak kek ae kb bik byen e M ya kk kak pi Hek WERA HER N kaka 20 Sample Pressure Calculations Converting Sample Pressures to Forces 20 Baseplate and Gasket Cleaning and Disinfecting keke eke ek 20 WayeformSlap LIEN WO NE ka ka l l n a a n bene a e nek ka a n kay k 22 Flexcell FX 5000 Compression System Specifications 24 Appendix FX 5000 Compression System Dual Baseplate Operation 27 E FLEXCELL INTERNATIONAL CORPORATION ONE YEAR LIMITED PARTS WARRANTY Flexcell FX 5000 Comp2. 2 3 4 5 6 Figure 15 FX 5000 regimen editor Choose the desired waveform shape under Shape by clicking on the downward arrow and selecting an entry from the drop down list Enter the desired minimum and maximum elongation for the plate membranes 0 30 in the boxes under Min and Max Enter the frequency for the waveform 0 01 5 Hz in the box under Freq If a static regimen is required enter the desired elongation at which the membrane is to be held in the Max box Enter 0 in the Min box and 1 0 in the Freq box Min and Freq are irrelevant for static regimens If a duty cycle for the waveform is to be defined enter the desired value in the box under DC The duty cycle is the percent of time that the waveform shape remains on at the high square wave or rising triangle part of the waveform Duty cycle only affects the triangle and square waves 12 7 8 9 Enter the number of times for the regimen to repeat 0 65 535 in the box under Cycles When moving out this box the box underneath dd hh mm ss will update to the calculated regimen time according to the set parameters The format for this entry is days hours minutes seconds However if the time is entered first the program will calculate the number of cycles To make a regimen step except for the first step return jump back to a previous regimen step enter the return step number in the box under Back To T
3. Figure 12 FX 5000 tutorial 3 3 MAIN DISPLAY WINDOW The main display window Fig 13 for each FX5K Compression FlexLink has several features that allow more precise monitoring of the regimen To access this window select the appropriate FlexLink FlexLink n from the Controllers menu where n is the FlexLink number to which the regimen will be downloaded The FlexLink number can be found on the LCD screen on the front of the FlexLink controller box All of the remaining information and command button states in the main window display reflect the current state of the system for the currently selected FX5K Compression FlexLink It is possible to simultaneously assign and run a different regimen for each of the attached FlexLinks In the upper left hand corner of the window is a section labeled Plot Units This function allows users to change the units of the y axis force Ibs or kPa To select a particular unit click on the appropriate button beside the unit label E FLEXCELL INTERNATIONAL CORPORATION P Fie ink 1 Compression l Phot Units Smoothing f None Moving Average Regmen Test as e J JERI Platform BioPress Compression Too wf anme j ee mn 0 00 oo 20 40 60 eo 100 Zo mo 1680 io ao e Assigned Figure 13 Main display window The next block to the right is a section labeled Smoothing One expects data patterns to r
4. Culture Plates and Baseplate Clamping Assembly setan vs il ol kt oi bk a ti u n k Ka H NAN A U DV K RA H ke daner KERALA na Ken nk ARO n Veke n AR 4 2 6 Connecting the System Positive Pressure LinmesS eee keke keke 6 2 7 FlexSoft FX 5000 Software and System Power up cssccssssssssssssesseesesssseeseseessees 7 2 8 LCD Display on the FX5K Compression FlexLink cccccsssesssesseessessseesseeseesseeees 7 2 9 Using Additional Flex Links eee ereke eee ereke ekek eke 8 3 Operation In StrilCi lO Sy i Gok ik ki bal Hi kak pa ecm Rees 9 3 1 Starting the FX 5000 Program a tetetretettetesosoesosoesosoosoososoososneunososoouoeooanesosnaanosous 9 3 2 Using the FX 5000 Tutorial kwit via ai foi pl kas t foi kaka kana an e ob 9 3 3 Main Display WindOW eke e keke konn paket tandans rans KAKE KRA KA KA 9 JA Configuring L IS ES ba ss al koki boko pasan ata ia ak a e st ae pa E A ak n R ae 11 3 5 Gfeating GO ANE TYE un a tn kek nn n ka ok en KR L a e A RE a SEE a ya S AA pe a A bh Sa WEKA 11 3 6 Creating a Regimen with a Custom Waveform Shape ekere 13 Dd POSS OTIS RSC MATEN 562 pce ian kaa kk in ak a a kk np cera a n n e pe te c nan s so 14 3 8 Starting Regil TS cx anaes kok pak twasan 444554134448 kan a wen ka b n M w ne n ba bek bke ke nos a l e H RE 15 3 9 Simulating Ml DI N at a l al e e ti a ak mm 15 3 10 Pausing RegimernsS
5. Flexcell FX 5000 mpression System 200 pm 12 15 14 Rev 13 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation e 2730 Tucker Street Suite 200 e Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 e www flexcellint com COPYRIGHT 2009 FLEXCELL INTERNATIONAL CORPORATION E FLEXCELL INTERNATIONAL CORPORATION TABLE OF CONTENTS One Year Limited Parts Warranty u eee ke keke kek ee kek kek ka keke kek Ka kr Ke KK KA KH iii Dell Computer Warranty Information keke keke ee kek kek ee kek kek k ke ke iv Federal Communications Commission Radio Frequency Interference Statement v FX 5000 Quick Start Instructions c c ccccsccccssessssssesssessesessesscsesarsucstsusssstsesstsessessnsaeseeanenees vi 1 Flexcell FX 5000 Compression System Components l 2 Setup of the Flexcell FX 5000 Compression System l AA PO Weta E xa ene err a n a E n n ks a n e D e ko ak ES l 2 2 Location of the FX 5000 Compression System c c csscsssssssseesessessessessessesesseseessees l 2 3 Setup of the Computer and FX5K Compression FlexLink ee 2 2A Incubator Access POTIS ii 4 545144aka a4a ini akena ki b zi ka z sa w b ena pa b na ka DE RAW ka asse a k s aaa aksan 3 2 5 Sample Preparation and Setup of BioPress
6. click on Pause in the main display window Click on Yes when the program asks for confirmation Pausing will cause the current simulation or actual running of the regimen to stop at the next cycle of the current regimen step Pausing is different from stopping in that pausing leaves the regimen at its current position whereas stopping causes the regimen position to be set to the beginning of the first regimen step 3 11 RESUMING REGIMENS To resume a paused regimen click on Resume in the main display window This function is available only when a simulated or running regimen has been paused The regimen will restart its prior activity simulation or actual run from its last point 3 12 STOPPING REGIMENS To stop a simulated or running regimen click on Stop in the main display window Click on Yes when the program asks for confirmation Stopping causes the current activity to cease immediately and sets the regimen position back to the beginning of the first regimen step Stopping is different from pausing in that pausing leaves the regimen at its current position so that it can be resumed from where it stopped 3 13 DETERMINE THE PROGRESS OF THE REGIMEN The progress of the regimen for the currently selected FlexLink will be monitored continuously The displays for Run Time Step number Cycle number Jump to number and Min and Max elongations will be updated in the upper right hand corner of the main display E FLE
7. and that the computer be allowed to run at full power during an experiment 2 8 LCD DISPLAY ON THE FX5K COMPRESSION FLEXLINK The front of the FX5K Compression FlexLink box has an LCD graphic display When the FX5K Compression FlexLink is turned ON the LCD will display the FlexLink number i c 1 2 3 or 4 and the regimen status i c Idle Ready or Left the time remaining in minutes of a E FLEXCELL INTERNATIONAL CORPORATION running regimen When a regimen has been downloaded and or is running the LCD will also display the user name platform and regimen name See Table 1 below for the display messages with various FlexLink statuses Table 1 Messages displayed on the FX5K Compression FlexLink LCD FlexLink Status LCD message Power ON 1 Regimen IDLE Flexcell International Corp www flexcellint com Regimen 1 Regimen READY downloaded User name Platform Regimen Name Flexcell International Corp www flexcellint com Regimen is 1 Regimen LEFT 5 min running User name or being simulated Platform Regimen Name www flexcellint com Flexcell International Corp Regimen has 1 Regimen IDLE stopped User name Platform Regimen Name www flexcellint com Flexcell International Corp 2 9 USING ADDITIONAL FLEXLINKS Using additional FXSK Compression FlexLinks will allow two or more baseplates platforms with different regimens to ru
8. the center screw can be turned counterclockwise x number of turns however it should be noted that the sample will no longer be fully compressible as the base of the sample holder will no longer be able to contact the base of the center screw Using sterile forceps turn the center screw clockwise the number of turns required by the above equation s Be careful not to screw the center down too far as this will preload the sample and give inaccurate force readings Add or remove fluid medium through any of the eight holes around the inner periphery of the platen Fig 7 Center the BioPress compression plates on top of the four black gaskets in the baseplate Fig 8 Adjust the eight clamping pads in the clamping system to their maximum height and turn them so that the bottom of each pad is parallel with the surface of the baseplate Place the Plexiglas window into the clamping system Fig 9 evenly along the bottom four clamping bars Baseplate without plates Baseplate with plates Figure 9 Clamping system with Plexiglas window NOTE The Plexiglas window is used as a backing support for the baseplate to enable it to withstand high pressures Never operate the compression baseplate without this window in place E FLEXCELL INTERNATIONAL CORPORATION Carefully slide the baseplate plate assembly into the clamping system so th
9. unit only 3 65 x 12 45 x 13 40 9 3 x 31 6 x 34 0 cm 115 230V 8 8 4 3A or 4 3 2 2A 60 50 Hz 15 0 Ibs 6 8 kg 10 5 x 7 0 x 18 5 26 7 x 17 8 x 46 9 cm 115 230V 6 3A 60 50 Hz 22 15 lbs 10 06 kg 14 6 x 15 65 x 7 1 37 1 x 39 75 x 18 0 cm 115V 60 Hz Auto switch to 230 V 11 22 lbs 5 1kg FX 5000 COMPRESSION COMPRESSED AIR REQUIREMENTS To achieve the maximum system capability the recommended compressed air requirements are e The pressure source must be able to produce 12 PSI 83 kPa of pressure at 0 9 cfm 25 5L min e If a house pressure system is being used and there are no means to test the flow rate capabilities run the house pressure system with the FX 5000 to determine if it is able to maintain 12 15 PSI 83 104kPa consistently while the FlexLink is running a regimen If it is able to maintain 12 15 PSI 83 104kPa while the FX 5000 is running then your house pressure source is sufficient e The following is an example of specifications for a floor compressor pump that can supply enough pressure to run the FX 5000 The pressure versus flow curve for this pump shows that it can achieve the necessary 12 PSI 83 kPa of pressure at 0 9 cfm 25 5L min Contact the manufacturer for the specifications of the floor compressor pump to determine if it will meet the necessary requirements o Maximum Pressure 60 PSI 414 kPa o Maximum Free Airflow Rate 1 1 cfm 31
10. where n is the FlexLink number to which the regimen will be downloaded In the Regimen menu select Assign then choose the Platform User and Regimen from each drop down menu Fig 16 A message box will appear to confirm regimen assignment Click Yes NOTE Be sure to choose the platform that properly corresponds to the setup being used 14 For the Flexcell FX 5000 Compression System the BioPress and StagePresser platforms will be used depending on the purchased device An error message will be given if an incompatible platform is selected P FX 5000 Flex ink 1 Compression Cortrcllers Congue Regmen Data Analysis Window Help asin 9 Figure 16 Assigning regimens for downloading to the FX5K Compression FlexLink If the download progresses no further messages will appear and the Start and Simulate buttons will be enabled If an error occurs during the downloading of the regimen to the FlexLink a message will alert the user of a problem and they will not be able to run the regimen In which case click on Reset in the FlexLink window and then assign the desired regimen again E FLEXCELL INTERNATIONAL CORPORATION 3 8 STARTING REGIMENS To run an assigned regimen click on Start in the main display window Fig 13 after the regimen has finished downloading Be sure that the positive pressure source is ON and all other connections are made The membranes shoul
11. L min 26 A FLEXCELL INTERNATIONAL CORPORATION Appendix A FX 5000 COMPRESSION SYSTEM DUAL BASEPLATE OPERATION E FLEXCELL INTERNATIONAL CORPORATION When using the FX 5000 Compression System in dual baseplate operation the user may notice a drop in the overall programmed Force depending upon the desired Force and frequency of the regimen This drop is due to the pressure source being shared between both baseplates It is therefore recommended that users reduce the frequency of such a regimen to 0 5 Hz or lower A lower frequency will allow the FX 5000 Compression System the correct amount of time to intake and exhaust the air which in turn gives the system enough time to reach the desired Force For example if an FX 5000 Compression System is connected in the dual baseplate configuration and a regimen is programmed with the following variables Waveform Sine Force 15 lbs Frequency 1 Hz The maximum Force achieved might only be 10 Ibs Reducing the frequency of the regimen to 0 5 Hz will produce an achievable Force of around 13 Ibs 28 E FLEXCELL INTERNATIONAL CORPORATION NOTICE The information in this document is subject to change without notice Flexcell International assumes no responsibility for any errors that may appear in this guide This manual is believed to be complete and accurate at the time of publication In no event shall Flexcell International Corporation be liable for in
12. PORTS Consult the operations manual of the CO incubator for the location of the incubator access ports Most incubators have an access port at the top or the rear of the incubator If this opening is not immediately apparent consult the incubator manufacturer about the location of an access port To prevent voiding the incubator warranty or encountering an electrical hazard a trained incubator representative should open the incubator access ports Once the access port has been located insert the FLEX IN and FLEX OUT tubing through the access port into the incubator Attach the 4 6 4 mm blue tubing to the 6 4 mm quick disconnect on the baseplate Attach the 9 5 mm clear tubing to the 36 9 5 mm quick disconnect on the baseplate Fig 4 If two baseplates are to be used simultaneously connect the baseplates using the configuration as shown in Figure 5 3 8 9 53mm tubing Ja il i j ET rin sh Ha 10 ft 3m 3 8 9 asa m 10 ft 3m 1 4 6 35mm tubing to Flex Out tubing to Flex In Figure 4 Single baseplate connection clamping system not shown T ji e tin gt 7 e J 10 fi Gm ME m 10 fe fan a areas m o Flex Figure 5 mT TT Tn for two mile damping systems not shown E FLEXCELL INTERNATIONAL CORPORATION 2 5 SAMPLE PREPARATION A
13. Regimen menu select Create new and create a regimen by entering values into the appropriate spaces or click on the Run Wizard button in the bottom left hand corner for a step by step procedure When completed click on Save Regimen Exit the regimen editor In Controllers menu select the appropriate FlexLink number to which the regimen will be downloaded In the Regimen menu select Assign Choose the proper platform user and regimen Important see section 3 7 Assigning Regimens from the drop down menus Confirm with a Yes to download the regimen Once the program is downloaded click on Simulate in the main window display Once satisfied that the regimen is correct click on Stop Turn ON the pressure source Do not allow the input pressure to exceed 20 psi or the transducers may be damaged Click on Start to run the regimen The plates should begin flexing To pause or stop the regimen click on Pause or Stop at any time Otherwise the program will stop once the regimen is complete vii E FLEXCELL INTERNATIONAL CORPORATION 1 FLEXCELL FX 5000 COMPRESSION SYSTEM COMPONENTS 1 Dell OptiPlex desktop computer 4 USB 2 button entry mouse Do not delete any software or change any 5 17 Flat Panel monitor settings on this computer It has been 6 Accessory pack nuts ferrules pre configured to operate the FlexSoft 7 Flexcell positive pressure baseplate FX 5000 software 8 Gaskets and clamping
14. a leak somewhere in the system First check all tubing to be sure that it is straight and not bent to restrict air flow To find the location of pressure leaks do the following Solution 1 Check the Flex In and Flex Out connections on the back of the FlexLink unit Be sure that these are connected properly and sealed tightly Next run a regimen and take note of the max and min forces shown on the screen Disconnect the quick disconnects from the baseplate and then connect them together bypassing the baseplate Take note again of the max and min forces They should increase slightly because of the decrease in system volume If the force outputs on the screen are now reaching the forces that you requested in your regimen then the leak is likely in your baseplate clamping system If the force output remains incorrect with respect to your regimen inputs check your input pressure 18 level from your pressure source Try increasing it slightly If the problem remains then the FlexLink may need recalibration Call Flexcell International for further instructions regarding recalibration To correct a leak problem in the baseplate try increasing the clamping pressure on the BioPress compression plates If this doesn t work remove the clamping pressure from the BioPress plates Remove the BioPress plates from the gaskets and check the indentation made by the plates on the gaskets to be sure that the plates were appro
15. and operating this device The equipment must be operated in strict accordance with the manufacturer s instructions The unit must be plugged into a properly grounded power outlet and used with the power cord supplied with the unit unmodified The unit must be operated with the factory installed cover in place No modifications of the equipment can be made which may result in the equipment failing to meet the limits of the FCC Rules The equipment must be maintained in a satisfactory state of repair Shielded cables must be used with this equipment to ensure compliance with the FCC Rules If the unit does interfere with radio or television reception which can be tested by turning the unit off and on the user is encouraged to try the following suggestions to correct the interference ROP OR q Reorient the antenna of the receiver Relocate the equipment to increase the distance between the unit and the radio TV Connect the unit to an outlet on a different circuit from the receiver Consult with the dealer or an experienced radio TV technician for advice on ways to reduce interference 10 11 E FLEXCELL INTERNATIONAL CORPORATION FX 5000 Quick Start Instructions Choose a location for the FX 5000 Compression system that is near the incubator Place the computer monitor keyboard mouse and FX5K Compression FlexLink on a sturdy flat surface Connect the keyboard mouse and monitor to the comput
16. by side with the keyboard and mouse in front The total weight of the system is 48 37 Ibs 21 96 kg Power Surge Protector Source Compressed Air Source Vacuum Line To SYSTEM Port Blue Tubing Computer FX5000 FLEX OUT Clear Tubing Baseplate FLEX IN Blue Tubing Incubator Access CO Incubator Figure 1 Flexcell FX 5000 Compression System Flexcell Intemational Corp 437 Dimmocks MI Rood Hi NG 27278 Inus oak 1 800 728 3714 Intemaflonct 1 919 722 1891 www flexcellint com PUMP CONTROL CONNECTION Figure 2 FX5K Compression FlexLink rear view Voltage Selector Switch Power Connector Ethernet Port Connect FX5K FlexLink 6 USB Ports 4 Available 7 1x Keyboard 1 x Mouse Monitor Connection Ethernet Port Figure 3 Dell OptiPlex rear view 2 3 SETUP OF THE COMPUTER AND FX5K COMPRESSION FLEX LINK Setup the Dell OptiPlex desktop computer and peripherals according to the manufacturer s instructions Refer to Figures 1 10 for proper setup of the FX 5000 system instruments Place the FX5K Compression FlexLink next to the computer and connect the FlexLink to the computer via the Ethernet cable provided If additional FlexLinks have been purchased they are to be placed side by side For multiple FlexLinks to be controlled by one comp
17. in the FX5K Compression FlexLink To be sure that the proper input pressure level is achieved when running the system first adjust the pressure level to 15 psi 103 kPa when the unit is not yet running a regimen This can be done using a pressure regulator or by adjusting the relief valve on the pressure output side of the pump if using a small floor pump Once the regimen has begun check to see how much the pressure drops on the gauge Readjust the pressure regulator or relief valve as necessary to get the input pressure between 12 and 15 psi 83 and 104 kPa while the regimen is running This will assure a proper pressure level for the unit to function correctly NOTE With optimal pressure settings there will still be some system limitations due to airflow Combinations of high frequencies and forces may not be achievable due to the high pressure level in the short period of time requested An example would be a regimen programmed to run from 0 12 pounds at 1 0 Hz The system is not able to achieve the pressure required to reach 12 pounds in 0 5 seconds because of airflow limitations Therefore the output may only reach 9 or 10 pounds The exact output is dependent on several system variables such as tubing and overall system volume To maximize system performance use the shortest tubing lengths possible see section 2 2 2 7 FLEXSOFT FX 5000 SOFTWARE AND SYSTEM POWER UP The FlexSoft FX 5000 software F
18. single sample in mm Given the force programmed into the software the pressure applied to the samples given can be calculated using the following equation Papa 5 65 Forcem Dmm where Pypa is the pressure applied to the sample in megapascals MPa Force is the force entered into the regimen or displayed on the software screen in pounds and D is the diameter of a single sample in mm 20 BASEPLATE AND GASKET CLEANING AND DISINFECTING The black urethane baseplate should not be autoclaved as the high temperature could cause warping The baseplate is not dishwasher safe because the high water temperature may cause warping The black rubber gasket should not be subjected to the high temperatures involved with autoclaving and or in dishwashers Both baseplate and gasket should be cleaned with regular laboratory detergent rinsed with warm tap water followed by distilled or deionized water and allowed to dry The baseplate should be dried and stored on a flat surface Do not hang the black gasket on pegs to dry as the gasket can be stretched Protect both pieces from UV light exposure To disinfect use a mild bleach commercial bleach solution such as Clorox or other laundry bleach solution is normally 5 25 sodium hypochlorite solution 1 500 bleach to water or a 1 benzalkonium chloride solution which can be sprayed poured or wiped on both pieces and allowed to stand on surfaces for at least 5 min Rinse wit
19. system 9 Plexiglas baseplate bottom support Preinstalled software 10 Inlet and outlet tubing FlexSoft FX 5000 11 Flexcell FX 5000 Compression Microsoft Windows 7 Microsoft Office 2010 Adobe Acrobat 2 FX5K Compression FlexLink 3 USB Keyboard System Users Manual and other ancillary manuals for computer and monitor 12 Surge protected outlet strip 13 Pop off valve Pressure gauge assembly 14 Positive pressure source Not supplied 2 SETUP OF THE FLEXCELL FX 5000 COMPRESSION SYSTEM Do not power up the Flexcell FX 5000 Compression system until all connections among components have been properly made and checked Never power up the FX5K Compression FlexLink with the cover removed an electrical shock hazard exists Operation of the computer without the cover is in violation of FCC regulations and will void the warranty 2 1 PACKING LIST Remove the packing list and check off the instrument components as they are removed from the shipping containers If any parts are missing contact Flexcell International Corp immediately Copyright 2009 Flexcell International 2 2 LOCATION OF THE FX 5000 COMPRESSION SYSTEM Choose a location for the FX 5000 Compression system Fig 1 that is adjacent and as close as possible to the CO incubator and positive pressure source A shorter distance in the pressure lines decreases the airflow resistance for compression and release of the fl
20. to 14 pounds of applied force to a tissue or 3D cell culture sample Minimum force resolution capability is 0 1 lb force Applies a defined controlled static or cyclic deformation to growing cells in vitro Housed valving mechanism automatically regulates and maintains pressure to provide the specified compression regimen Capable of driving up to 4 independent FlexLink remote compression and or strain controllers Capable of delivering frequency ranges from 0 0135 Hz Works with BioPress series culture plates Able to program multiple frequency amplitude and wave changes in one regimen Waveforms available Static wave Sinusoidal wave Heart wave Electronic and Pressure Triangular wave Square wave and custom waveforms Optional StagePresser device allows compression application to cells while viewing in real time under an upright microscope FX 5000 COMPRESSION COMPONENTS AND SPECIFICATIONS Dell OptiPlex Desktop Computer USB Keyboard USB Mouse 17 Flat panel Monitor FlexSoft FX 5000 V1 0 Flexcell FX 5000 Software Microsoft Windows 7 FX5K Compression FlexLink controller Accessory Pack nuts ferrules Flexcell positive pressure baseplate gaskets clamping system and Plexiglas baseplate bottom support Inlet and outlet tubing Flexcell FX 5000 Compression System User s manual Culture Plate amp Loading Stations manual and other ancillary manuals for computer and monitor Surge protected power o
21. ND SETUP OF BIOPRESS CULTURE PLATES AND BASEPLATE CLAMPING ASSEMBLY The BioPress compression culture plate contains a foam ring and sample holder in each well Fig 6 The foam ring is used to contain the removable sample holder In preparing samples be sure that the sample thickness is no less than 1000 um and no greater than 0 118 3 mm The thickness of the compressed foam is 350 um therefore thinner samples will not be compressed A sample thickness of 1000 um or more will ensure compressibility The maximum chamber height is 0 122 3 1 mm therefore samples with a thickness greater than 0 118 3 mm will be preloaded when the platens are inserted into the wells of the culture plates Figure 6 Compression culture plate foam ring and sample holder The sample diameter should be equal to or less than the inner diameter of the foam sample holder in the chamber which is 0 200 5 mm If the user wishes to use a larger sample they can remove the sample holder and place the sample within the larger ring that normally contains the sample holder This will allow a sample diameter of up to 0 525 13 mm To prepare a sample for loading remove the sample holder from the plate using a pair of sterile forceps Place the tissue or gel sample into the hole at the center of the removable sample holder Carefully place the sample holder back into the BioPress compression plate so that it is contained
22. Users box so that it is highlighted in blue then click on Delete User The program will ask for a confirmation and issue a warning click Yes to confirm deletion The user and any of the user s defined regimens will be deleted To exit the Users window click on Close 3 5 CREATING REGIMENS Before beginning a regimen must be created for downloading In the Regimen menu select Create new A new window will appear entitled Regimens with a list of the existing users and their regimens Fig 15 In the Existing Users box click on the user name under which the regimen is to be created A list of current regimens created under this user will appear in the Regimens for Selected User box unless no regimens have been saved under this user in which case this box will be empty To create a new regimen click on Run Wizard in the bottom left hand corner This option will bring up a step by step process of creating a new regimen Alternatively follow the sequence of steps below 1 Run Weard E FLEXCELL INTERNATIONAL CORPORATION Type a regimen name in the box labeled Regimen Name Regimens for Selected User Test Step 1 Duration 0 01 01 00 DC Cycles o 0 fa dd hh mm ss Back To Repeat 0 00 00 30 fo fo ri OC Cydes Duration BackTo Repeat Pump 5 r r 0 0 00 59 00 0 0 30 3 00 00 50 0 30 0 00 00 30 50 0 3600 0 01 00 00 w a Add Step Remove step Save Regimen Delete Regimen
23. XCELL INTERNATIONAL CORPORATION window The actual waveform data returned by the controller will also be continuously updated on the graph A timeline progress bar below the plot illustrates the percent of the regimen that has been completed 3 14 RESETTING THE FLEXLINK Resetting a simulated or running regimen causes the current activity to stop at the next cycle of the current regimen step and disassociate the regimen from the FlexLink controller To reset click on Reset in the main display window Click on Yes when the program asks for confirmation The system will now be in state similar to that which exists when the FX5K Compression FlexLink is first turned ON 3 15 USING THE PRESSURE FORCE CONVERSION CALCULATOR To convert pressure kPa to force Ibs or vice versa select Calculate from the Regimens menu To select the Convert Pressure to Force or Convert Force to Pressure calculator Fig 17 click on the appropriate button beside the calculator label Calculate Convert Pressure to Force Pressure kPa Sample Diameter mm Force Ibs fo 1 a Convert Force to Pressure Force Ibs Sample Diameter mm Pressure kPa Calculate Figure 17 Calculator for converting pressure to force or vice versa For pressure to force conversions enter the pressure in kPA in the Pressure kPa box and the sample diameter in millimeters in the Sample Diameter mm box Clic
24. at the four BioPress plates are centered underneath the eight clamping pads Fig 10 Check the Plexiglas window and move as necessary to center it under the baseplate Turn the wing nuts on the clamping pads until they are finger tight This will compress the plates downward onto the gaskets creating a seal Cpe Won lt gt Baseplate slides into clamping system Plexiglas ty 2 sy window on bottom and then l W S lt T tighten the clamps down over S2 3 the compression plates Do not over tighten a ee A Plexiglas window must fit under the baseplate to keep seal Figure 10 Clamping system with baseplate and culture plates NOTE If using less than 4 plates in an experiment use an old plate or support in place of the missing plate s and tighten the clamping pads onto this surface This will ensure that the baseplate is seeing an even and sufficient pressure across its surface and will prevent baseplate blowout Place the entire assembly into the incubator Attach the FLEX IN and FLEX OUT quick disconnect fittings to the fittings on the compression baseplate Fig 1 amp 4 2 6 CONNECTING THE SYSTEM POSITIVE PRESSURE LINES Connect a minimal length of the blue 9 5 mm tubing to the positive pressure source The pressure source should be capable of maintaining a pressure of 12 15 psi 83 104 kPa when the FlexLink is running Most house pressure systems will be ca
25. cidental or consequential damages in connection with or arising from the use of the manual Opening the case of the FX5K Compression FlexLink voids the warranty Customers must contact Flexcell International Corporation or an authorized distributor before opening the case on the FX5K Compression FlexLink 29
26. d begin flexing and the assigned regimen will be in progress The progress of the regimen for the currently selected FlexLink will be monitored continuously The displays for Run Time Step Cycle Jump and Min and Max will update as will the actual waveform data returned by the FlexLink Additionally a progress bar below the graph will display the percentage of the regimen that has been completed 3 9 SIMULATING REGIMENS The Simulate button is used to simulate a regimen graphically without actually running it Simulating a regimen will give the user an idea of the waveform output before actually running the regimen To start a simulation click on Simulate in the main display window A waveform will begin moving across the graphic display This waveform corresponds to the ideal waveform as generated by the FX 5000 software and is the actual waveform pattern sent to the FlexLink during the download process For certain waveforms e g square waves the idealized pattern may appear to have some unusual elements e g rounded corners The purpose of these unusual elements is to accommodate for the lag time during sudden changes in pressure e g rising falling edge of a square wave Without these unusual elements the valve controllers may oscillate for a period of time e g dampened sine wave during beginning of plateau in a square waveform 3 10 PAUSING REGIMENS 15 To pause a simulated or running regimen
27. enly along the bottom four clamping bars NOTE This window is used as a backing support for the BioPress baseplate to enable it to withstand high pressures Never operate the compression baseplate without this window in place Slide the BioPress baseplate plate assembly into the clamping system and adjust so that the four plates are centered underneath the eight clamping pads Check the Plexiglas window below and move vi 12 13 14 15 16 17 18 19 20 21 E FLEXCELL INTERNATIONAL CORPORATION as necessary to center it under the BioPress baseplate Tighten the pads down until the wing nuts are finger tight At this point the BioPress plates should be sufficiently clamped NOTE If using less than 4 culture plates in an experiment put an old plate or support in place of the missing plate s and tighten the clamping pads onto this surface Place the baseplate clamping system assembly into the incubator Insert the 1 4 6 4 mm blue and 3 4 9 5 mm OD clear tubing into the access port of the incubator Snap the quick disconnects for the 1 4 6 4 mm and 34 9 5 mm tubing to the quick disconnects on the baseplate Turn the FlexLink and computer system ON The Windows desktop should appear on the monitor Double click on the FX 5000 icon on the left hand side of the screen to start the software In the Configure menu select User Editor and register as a user In the
28. epresent normal distributions of a function describing a straight line sinusoidal curve etc Therefore the plotted points of a mathematical function should be a predictable data set that is smooth i e a curve comprised of points whose coordinates are predicted by the function For a straight line the equation would be y mx b for a power function the equation may be y x or some derivative thereof In general data patterns may be confounded with noise For the case of the pressure plot as a function of voltage from the FX 5000 Compression System pressure transducer the rough data yield a plot with a coefficient of variation of only 0 01 Therefore the signal to noise ratio for the combined Flexcell system of circuits hardware and transducers is high lending confidence in the displayed plot Nevertheless the user can select a data smoothing option that is a simple weighted average of the points The Smoothing section contains the option of either applying no smoothing None to the data points returned from the FlexLink or of applying a three point moving average filter Moving Average to the incoming data Choose the desired affect by clicking on the appropriate button 10 Below the box for Smoothing is a section used to show the current X and Y coordinates for the waveform These are displayed when the left mouse button is clicked and held down in the graph area When this is done a pair of crosshair
29. er according to the manufacturer s instructions Connect the FlexLink COMM to the Ethernet port on the back of the computer using the Ethernet cable supplied see Figs 2 and 3 p 2 Connect the computer monitor and FlexLink to a power source using a surge protector Connect the system pressure line to the SYSTEM port on the back of the FlexLink using the 34 9 5mm OD blue tubing Cut the SYSTEM tubing near the unit and add the pop off valve and pressure gauge assembly in line with the tubing Cut a 10 3 m length of the 4 6 4 mm blue and 9 5 mm clear tubing and connect each to the back of the FlexLink in the FLEX IN and FLEX OUT ports accordingly Attach the provided quick disconnects to the other end of each piece of tubing Place the tissue gel samples onto the foam disks in the center of each well of the four BioPress compression plates Add 3 5 mL of medium into each well Place a stationary platen into each well of the plates and screw the center piece down until it touches the top of the tissue gel sample Center the four BioPress plates on the four black compression gaskets in the baseplate Adjust the clamping pads to their maximum height on the compression clamping system Be sure that each pad surface is parallel with the surface of the BioPress baseplate to prevent any of the pads from catching the plate lids during insertion Place the Plexiglas window into the clamping system ev
30. exible bottomed plates allowing for a greater range of compression at higher frequencies The length of the pressure line should be a minimum of 4 1 2 m All cables mouse monitor keyboard and Ethernet communications cables will connect to the rear of the computer Fig 3 All tubing and the Ethernet communications cable connect to the rear of the FX5K Compression FlexLink Fig 2 The length of the clear and blue tubing from the FlexLink to the baseplate should be 10 3 1 FLEXCELL FlexLink and FlexSoft are trademarks of Flexcell International Corporation MS Windows and MS Office are trademarks of the Microsoft Corporation Adobe Acrobat is a trademark of Adobe Systems Inc Dell OptiPlex is a trademark of Dell General Notice Other product names mentioned in this manual are for identification purposes only and maybe trademarks of their respective owners Flexcell International disclaims any and all rights in those marks E FLEXCELL INTERNATIONAL CORPORATION m long This length can vary if necessary but no more than 5 1 5 m longer or shorter Cut the tubing after the system is fully set up to ensure of the proper length NOTE The PUMP CONTROL CONNECTION Fig 2 feature is only available with the FX 5000 Tension system The FX 5000 Compression system will require a space 28 75 73 03 cm wide by 22 44 57 00 cm deep if the computer FX5K Compression FlexLink and monitor are placed side
31. filename 2 the data presented defaults to a moving average of the raw data with data averaged every 10 seconds E FLEXCELL INTERNATIONAL CORPORATION Figure 18 FX 5000 data display The y Axis control box allows for automatic or manual control of the scale on the y axis If Auto is selected the y axis scale can be changed by clicking on one of the two small double arrow buttons at either end of the axis The button with arrows pointing away from each other increases the axis scale while the button with arrows pointing toward each other decreases the scale The minimum value is 2 kPa and the maximum is 100 kPa If Manual is selected the user can enter the upper and lower limits of the y axis scale in the white boxes to the left side of the axis The small double arrows will not function if Manual is selected The x axis scale allows for a time interval to be set between 0 1 and 1000 seconds Click on one of the two small double arrow buttons at either end of the axis to change this scale The button with arrows pointing away from each other increases the axis scale while the 17 button with arrows pointing toward each other decreases the scale The Input data box allows the user to switch the data in the graph from raw data to averaged data If the Raw data option is selected the graph will plot the pressure in kPa over time in seconds for the selected regimen If the Moving average option is selected t
32. gives you specific legal rights and you may also have other rights that vary from state to state 3 INTERNATIONAL CUSTOMERS The full text of the foregoing limited warranty and all disclaimers is applicable to international customers purchasers except when the purchase was made from an international distributor or reseller the warranty will be covered through your distributor or reseller If technical advisory support service is not available through your distributor or reseller for service contact warranty headquarters by phone or fax Within the United States only 1 800 728 3714 toll free Fax 1 919 732 5196 International Customer s telephone 01 919 732 1591 Fax 01 919 732 5196 Email info flexcellint com FLEXCELL INTERNATIONAL CORPORATION 2730 Tucker Street Suite 200 Burlington NC 27215 1 800 728 3714 within the U S only 1 919 732 1591 fax 1 919 732 5196 http www flexcellint com Customers should contact Dell Inc for all warranty questions or concerns with regards to the Dell OptiPlex computer and monitor iii E FLEXCELL INTERNATIONAL CORPORATION Dell COMPUTER WARRANTY INFORMATION Dear Customer Congratulations on your purchase of the Flexcell FX 5000 system This system comes with a one 1 year warranty The Dell computer that is included with this equipment will be transferred into the Principal Investigator and University s name at the time of purchase This computer comes with an excellent warran
33. gn a regimen Solution 6 This problem is usually due to a lack of communication between the computer and one or more FX5K Tension FlexLinks Check the FX5K Tension FlexLink Ethernet port connection on the computer and the communications connection on the back of the FlexLink to be sure that these are secure Check the FlexLink to see if the power is ON the FlexLink screen should power on If both of these conditions are true close and restart the software Try downloading the regimen again If the problem is not resolved call Flexcell for further instructions E FLEXCELL INTERNATIONAL CORPORATION 5 APPLICATION NOTES SAMPLE PRESSURE CALCULATIONS CONVERTING SAMPLE PRESSURES TO FORCES The actual pressure applied to a sample in the well of the BioPress compression plate is dependent on the surface area of the sample to which the force is being applied Therefore the FX 5000 Compression System is designed to apply a force to 3 D samples so that force is not dependent on sample size To convert a pressure to a force input the desired pressure to be applied to the sample into the equation below along with the sample diameter and program the resulting force into the FlexSoft software Forcems 0 177 Pupa Dmm where Force is the force applied to a single sample or cell in pounds Pmpra is the pressure to be applied to the sample in megapascals MPa and Dmm is the diameter of a
34. h distilled or deionized water The above solutions are effective against yeast and fungi A 70 ethanol solution also can be applied and pieces allowed to air dry do not wipe dry Tubing on the baseplate may be flushed with 70 ethanol but be sure tubing is completely dry before connecting to the Flexcell Unit Contact time time surface remains wet of the above solutions is critical to the effectiveness of disinfecting Times given are minimal and longer contact times are recommended in cases where contamination is severe E FLEXCELL INTERNATIONAL CORPORATION The above decontamination procedure should be performed before and after each use of the baseplate and gasket or whenever media is spilled onto these surfaces Be sure that the openings and the channels in the baseplate are dry and clear of any obstructions before using 21 Do not store baseplate or gasket in incubator as the warm humid conditions will promote growth of microorganisms After cleaning and disinfecting seal baseplate and gasket in a plastic bag Store baseplate flat and be sure that the entire bottom of baseplate is supported to prevent warping Store gasket away from UV light exposure E FLEXCELL INTERNATIONAL CORPORATION WAVEFORM SHAPE DEFINITIONS 14 Sine Wave Sine Wave This waveform provides a smooth This waveform provides a smooth physiological variation in pressure at the physiological variation in pressure from min max with a quick
35. he graph will plot the average pressure over the designated time period in seconds range of 0 1 1000 seconds shown in the Time s box The Start time box can be set so that the data viewed in the plot begin at the specified time entered here Use the timeline at the top of the graph to navigate through the regimen Users can move the slider function within the timeline to manually progress through the regimen plot If the user clicks on the arrows at either end of the timeline the plot will progress to the next time period as set by the x axis scale For instance if the scale is set to show 20 seconds of data and the user clicks on the right arrow button the plot will progress to the next 20 seconds of data 3 17 EXITING THE FX 5000 PROGRAM To exit the FX 5000 software in the Controllers menu select Exit E FLEXCELL INTERNATIONAL CORPORATION 4 SYSTEM TROUBLESHOOTING NOTE If your FX 5000 system is not functioning properly and the solution is not clearly outlined or defined below please contact Flexcell before proceeding with any repair work Problem 1 Gaskets on baseplate are not sealing Symptoms a System is not performing to specified parameters i e not reaching maximum elongation entered in regimen b Baseplate is making a whistling or blowing sound when air pressure is added Test to determine if this is the problem If you are experiencing either of the above symptoms there is likely
36. hen type the number of times to repeat this process in the box under Repeat For instance if the user was entering Step 4 and wanted to return to Step 2 and repeat Steps 2 through 4 three times enter 2 under Back To and 3 under Repeat when inputting Step 4 When the regimen step is complete click on Add Step at the bottom Every time a new edited step is saved the overall duration of the regimen will be calculated and displayed in the box labeled Duration next to the Step number box If the total duration exceeds the maximum allowable duration this value will be MAXIMUM and the regimen cannot be saved Adjust the time and or cycle number to decrease the total duration time 10 To add other steps to a regimen click in the box labeled Step and type in the next step number For example the first step will automatically be Step 1 To add another step delete the 1 and replace it with a 2 E FLEXCELL INTERNATIONAL CORPORATION After this is completed change the parameters as needed for the new step according to steps 2 to 9 above 11 To delete a step that has already been added click on the step in the window labeled Regimen Steps which contains the list of steps This will cause the step to be highlighted Then click on Remove Step at the bottom of the window Confirm the deletion by clicking on Yes when prompted 12 After all steps and parameters have been entered click on Save Regimen 13 To delete a
37. ial damages based upon breach of warranty or contract or negligence Such damages include but are not limited to loss of profits revenue loss of data down time customer s material or time f DISCLAIMER OF WARRANTIES The Limited Warranty expressed in the foregoing language is the only warranty applicable to this product Any other warranty expressed or implied warranty or of merchantability or fitness for a particular purpose are hereby disclaimed No oral or written information or advice provided by Flexcell through its agents or employees in the use and functioning of the equipment shall in any way create a warranty or in anyway increase the scope of this limited warranty g DISCLAIMER LANGUAGE This warranty document accompanying instruction manual and supplemental applicable laws appear in the English language In the event of any inconsistency in the meaning of the words and terminology and any foreign language translation the English language shall prevail 2 GOVERNING LAW The performance of the duties and liabilities of the parties under the terms and conditions of this Limited Warranty shall be governed in all respects by the laws of the State of North Carolina the United States of America APPLICATION OF STATE LAWS Some states do not allow the exclusion or limitation of consequential damages nor do some states allow limitations on how long an implied warranty lasts so the above limitations may not apply to you This warranty
38. itive E FLEXCELL INTERNATIONAL CORPORATION pressure is being applied to the membrane to compress it upwards against an immovable barrier the platen The scale on the x and y axes can be changed by clicking on one of the two small double arrow buttons at either end of the axis The button with arrows pointing away from each other increases the axis scale while the button with arrows pointing toward each other decreases the scale Below the plot is a timeline that displays the percentage of the regimen that has been completed To the right of the plot is a set of command buttons which allow the user to 1 Start a regimen 2 Stop a regimen 3 Pause a regimen 4 Resume a paused regimen 5 Simulate a regimen 6 Reset the controller 7 Access Help 8 Cancel the program 3 4 CONFIGURING USERS Prior to creating and downloading a regimen a user needs to be registered Once this task is completed regimens can be personalized under the registered user name In the Configure menu select User Editor A new window will appear entitled Users Fig 14 To add a user type in a user name must be at least 4 characters in the provided space and click on Add User The new name should appear in the Existing Users box Existing Users Flexcell Shutdown User Name Flexcell Delete User Figure 14 FX 5000 user editor To delete a user click on the appropriate user name in the Existing
39. k 16 Calculate The calculated force in lbs will appear in the Force lbs box For force to pressure conversions enter the force in lbs in the Force lbs box and the sample diameter in millimeters in the Sample Diameter mm box Click Calculate The calculated pressure in kPA will appear in the Pressure kPa box To exit this window click on Close 3 16 DATA ANALYSIS The FX 5000 software has the capability of storing data a set of waveform points from a regimen run to a file on the hard drive The filename consists of the FlexLink number a V or C code letter V for vacuum or C for compression the regimen name and a sequence number If a compression regimen is ran three times on FlexLink 1 then there will be three data files on the hard drive with numbers 000 001 and 002 i e 1_C_regimenname_001 dat 1_C_regimenname_002 dat etc The file size depends on the regimen duration The current settings take pressure data every 2 milliseconds i e 500 points sec or 1 800 000 points hour Therefore a one hour regimen will have approximately a 4 0 Mb data file To turn this feature OFF please contact Flexcell To see the stored data in the Data Analysis menu select Data Viewer Open the appropriate dat file from the C FlexSyst directory Two new windows will appear Fig 18 In the window labeled filename 1 the data presented defaults to the raw data In the window labeled
40. lexcell Copyright Protected Software Property of Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 has been pre installed on the hard drive Keep the CD package included with the system and store it in a safe place Be sure to shutdown Windows properly by using the Shut Down command activated by the Start button Failure to follow the above directions can cause software and hardware malfunctions The computer has Windows XP installed on the hard drive All backup software is provided with the system Check all connections and connect the surge protector into an appropriate power outlet 115 VAC or 230 VAC Turn ON the surge protector strip and check that the indicator light on the strip is lit If the power light does not come on the surge protector has failed Replace the surge protector and attempt to determine what caused the surge protector to fail Turn ON the power switch on the computer monitor and FX5K Compression FlexLink The system should then boot and load Windows Select and double click on the FX 5000 icon NOTE Flexcell recommends that the power saving or screen saver features in the Windows software are disabled Allowing the hard drive to power down while an experiment is running may create the need to reboot the computer before data can be viewed or before a regimen can be downloaded again It is recommended that the monitor be turned OFF when not in use
41. n simultaneously All tubing connections made to the first FlexLink should be made exactly the same with any additional FlexLinks Each FlexLink will have its own separate FLEX IN and FLEX OUT tubing for each baseplate However the pressure or SYSTEM tubing can be shared between FlexLinks with T fittings as long as the pressure source is capable of running two or more FlexLinks with the programmed regimens Regimens with a combination of high frequencies and force levels will require a more powerful pressure source The capability of the pressure source with more than one FlexLink will need to be tested experimentally with the particular setup FlexLinks must be placed side by side during use To connect additional FlexLink s to the computer connect the Ethernet cable from each FlexLink to an Ethernet hub Then connect the hub to the Ethernet port on the back of the computer using the Ethernet communications cable provided The LCD on the front of the FlexLink will display the number 1 4 that the FXSK Compression FlexLink has been assigned at the time of production NOTE The numbering of each FX5K Compression FlexLink will not depend on what order it appears in the connection chain but according to the configuration of each FlexLink as FlexLink 1 FlexLink 2 etc during production When powering up the FlexLink s and computer turn the FlexLink s ON before the computer T
42. nk Contact Flexcell for further instructions Problem 4 You want to program a long regimen 1 day or more and the software will not allow you Solution 4 The software has a limit on the number of cycles that can be entered into any given step 65 535 see section 3 2 5 7 of the manual If you program a regimen with a step that requires more than 65 535 cycles the software will give you an invalid entry error To run the regimen for the time required you will need to make an additional step This will not create any breaks in the regimen Simply divide your total time in half or as many times as is necessary and make additional steps with the same parameters See section 3 2 5 of the manual for instructions on programming additional steps 19 Problem 5 An invalid argument was encountered error is shown when attempting to assign a regimen Solution 5 This problem is caused when a user name is entered into the software without any regimens listed under his her name If the assign function sees that a user has nothing to assign an error is displayed Simply delete the user s without a regimen or make and save a regimen under the user s name s Problem 6 One ot more of the FX5K Tension FlexLinks connected to the system are not being recognized by the software This is usually represented by the etror message Cannot Connect to Controller when attempting to connect a FlexLink to assi
43. o use a particular FlexLink select the appropriate FlexLink number 1 2 3 or 4 from the Controllers menu Assign download and run regimens just as would be done when using one FlexLink CA FLEXCELL INTERNATIONAL CORPORATION 3 OPERATION INSTRUCTIONS 3 1 STARTING THE FX 5000 PROGRAM The Flexcell system should now be set up and ready for operation Be sure that the system positive pressure supply is adequate to operate the instrument s see section 2 6 Turn ON the power switch on the computer monitor and FX5K Compression FlexLink The system should then boot and load Windows Select and double click on the FX 5000 icon Fig 11 on the desktop to start the FlexSoft FX 5000 software A main display window will appear see Fig 13 A Fa SOO Figure 11 FX 5000 icon 3 2 USING THE FX 5000 TUTORIAL In the Help menu click on View Tutorial to view the FX 5000 tutorial at any time Fig 12 The tutorial will appear on the left side of the screen The tutorial explains the main steps involved in configuring users creating regimens and starting regimens The tutorial will start automatically as a default setting when the software is opened To turn OFF this feature uncheck the box marked Show Tutorial at Startup Controllers Configure Regimen Data Analysis Window Help Welcome to FX 5000 Tutorial Configure Users Create Regimen Start Regimen
44. pable of maintaining this pressure If a floor pump is to be used the following specifications are recommended as a minimum Max Pressure 60 psi 414 kPa Max Free Airflow Rate 1 1 cfm 31 L min If the pump does not meet one of the above specifications there is still a possibility that it will have the capability of providing the necessary pressure and flow rate combination to run the FX 5000 Compression System If the pump is capable of producing a minimum of 0 9 cfm 25 5 L min at 12 psi 83 kPa the pump can be used with the FX 5000 Compression System Check with the pump manufacturer to determine if the pump is capable of meeting these specifications NOTE Flexcell highly recommends the use of a pressure reservoir when using a floor pump as the positive pressure source Contact Flexcell for information on purchasing a pressure reservoir Keep the length of tubing between the pressure source and FlexLink as short as possible or 1 2 m minimum If the pressure source does not have an adjustable regulator built into it a pressure regulator will likely required to keep the input pressure to the system at an acceptable level recommended at 12 15 psi 83 104 kPa no greater than 20 psi 140 kPa Most house pressure systems will produce E FLEXCELL INTERNATIONAL CORPORATION pressures up to several hundred pounds per square inch 1 MPa or more Such pressure levels will damage the pressure transducers
45. regimen simply double click on the desired regimen name in the Regimens for Selected User box and then click on Delete Regimen Confirm the deletion by clicking on Yes NOTE If the regimen to be deleted is currently assigned the regimen must be removed from assignment before deleting 14 When finished with regimen editing click on Close 3 6 CREATING A REGIMEN WITH A CUSTOM WAVEFORM SHAPE In the top row of an Excel spreadsheet enter the elongation values for the desired waveform shape for one cycle Enter one value per cell Values should be chosen along a curve at even time intervals i e every 100 msec milliseconds Save this spreadsheet as a csv file comma delimited file format in the C FlexSyst directory Example Custom waveform shape waveform shape Ramp 13 Elongation 0 100 200 300 400 500 600 700 800 900 1000 Time msec Example Excel file Ramp waveform shape 1 1 2 2 3 3 4 4 5 5 10 5 5 5 5 5 Example CSV file viewed in Notepad Ramp waveform shape 1 1 5 2 2 5 3 3 5 4 4 5 5 5 5 0 In the Regimen menu of the FX 5000 software select Create new A new window will appear entitled Regimens with a list of the existing users and their regimens In the Existing Users box click on the user name under which the regimen is to be created A list of current regimens created under this user will appear in the Regimens for Selec
46. ression System 1 FLEXCELL INTERNATIONAL CORPORATION warrants to the original purchaser customer all hardware components of the Flexcell FX 5000 Compression System serial for a period of one year from the date of delivery to the purchaser customer to be free from manufacturing defects in workmanship or materials with the following exceptions terms and conditions a ITEMS EXCLUDED FROM THE WARRANTY ARE Dell OptiPlex desktop computer and monitor software disks manuals and external peripherals such as printers mouse or track ball units imaging devices vacuum pumps air tanks electric voltage converters compressors surge suppressers and all other accessory equipment b DURING THE WARRANTY PERIOD the purchaser customer must notify Flexcell of any warranty claim in writing by telephone fax transmission or email identifying each defective part or specifically describe the exact problem no later than the last day the warranty is in effect C FLEXCELL AGREES to correct any defect in workmanship or material and supply new or rebuilt parts in exchange for defective parts upon completion and submission by purchaser customer of a printed Parts Return Authorization form furnished by Flexcell Parts must be properly packed in original container and shipped to our factory service center or distributor with all shipping costs prepaid if the unit is out of warranty coverage If the original shipping box is not available Flexcell will send
47. s appears and the coordinates at the intersection of the crosshairs are shown in the X and Y spaces The crosshairs and values will track the mouse as long as the left mouse button is held down The next block to the right is a section used to display the current User Regimen Platform and Run time Run time has two blocks The one on the left displays the elapsed regimen time and the one on the right displays the remaining regimen time using the following format dd hh mm ss days hours minutes seconds The block at the top right contains the current regimen Step Cycle Jump when a regimen step is using the Jump Back to option minimum level Min and maximum level Max The minimum and maximum levels will be listed according to the units specified under the Plot Units section as the minimum and maximum points at which the membrane stretches with each cycle The major portion of the main display window is taken up with a plot designed to show the current state of a simulated or running regimen This plot is generated from the data returned by the FlexLink during regimen activity When using a platform configuration that uses positive pressure to generate an upward movement of the membrane resulting in compression the vertical scale represents increasing compression force When the selected unit of display is kPa the vertical scale will start at 0 0 and move upwards as an increasing positive level indicating that pos
48. ted User box unless no regimens have been saved under this user in which case this box will be empty To create a new regimen click on Run Wizard in the bottom left hand corner This option will bring up a wizard to create a new regimen Or follow the sequence of steps below 1 Choose Custom from the list of waveform shapes under Shape by clicking on the downward arrow and selecting this entry from the drop down list 2 Choose the appropriate file name under File by clicking on the downward arrow E FLEXCELL INTERNATIONAL CORPORATION and selecting an entry from the drop down list 3 Type a regimen name in the box labeled Regimen Name 4 Enter the time interval in milliseconds between two elongation values in the box under Time 5 Enter the number of times for the regimen to repeat 0 65 535 in the box under Cycles 6 When the regimen step is complete click on Add Step at the bottom Every time a new edited step is saved the overall duration of the regimen will be calculated and displayed in the box labeled Duration next to the Step number box If the total duration exceeds the maximum allowable duration this value will be MAXIMUM and the regimen cannot be saved Adjust the time and or cycle number to decrease the total duration time 7 Click on Save Regimen 8 When finished with regimen editing click on Close 3 7 ASSIGNING REGIMENS In the Controllers menu select the appropriate FlexLink n
49. the required protective shipping container Flexcell will recommend the insurance value for parts or equipment to be shipped Return carrier shipping costs will be paid by Flexcell from the service center The purchaser customer is solely responsible for payment of custom fees taxes holding fees or value added taxes d THIS LIMITED WARRANTY only covers failures due to defects in materials or workmanship which occur during normal use It does not cover damage which occurs in shipment or failures of original equipment due to products identified as add ons not manufactured by Flexcell International Corporation or its distributors nor does this limited warranty cover damages or failures which result from accident or disaster such as fire explosion flood wind lightning or earthquake or misuse abuse neglect mishandling misapplication alteration faulty installation modification or service by anyone other than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty This warranty is extended only to the original purchaser customer unless a transfer of ownership is approved by Flexcell in writing e LIMITED LIABILITY Flexcell or its distributor s only liability shall be to remedy any defect to comply with its warranty and return the repaired equipment to function as designed Under no circumstances shall Flexcell or its distributors be liable for any special incidental or consequent
50. transition at the min to max P Flex ink 1 Compression P FlexLink 1 Compression i 3 05 i 1 5 2 0 2 5 3 0 35 j 45 so tee g 05 E 1S F 25 ze 35 F 45 so e al Time Sec oj e I Time Sec Fi le Enn Enn Triangle Wave Square Wave This waveform provides a linear ramped rise This waveform provides the fastest possible to the max and a linear ramped fall back to rise to max followed by the fastest possible the min fall to min P Flext ink 1 Compression me P Flext ink 1 Comptession ok 25 Time Sec 22 E FLEXCELL INTERNATIONAL CORPORATION Heart E Wave Heart P Wave This waveform applies a pressure pattern that This waveform applies a pressure pattern that mimics the shape as seen on an mimics the actual pressure wave generated by electrocardiogram a heart beat F Flext ink 1 Compression lal F Flext ink 1 Compression Wok 25 Time Sec Static Wave This waveform applies a constant state of pressure F Flext ink 1 Compression Wo 0 0 0 5 10 1 5 20 25 3 0 35 4 0 45 5 0 pese EDI Time Sec ej Grad 23 A FLEXCELL INTERNATIONAL CORPORATION Flexcell FX 5000 Compression System Specifications E FLEXCELL INTERNATIONAL CORPORATION FX 5000 COMPRESSION SYSTEM FEATURES Uses air pressure to deform a flexible bottom culture plate yielding up
51. ty through Dell If you have any problems with this computer you will contact Dell directly The details of the warranty are listed below You will need to have the service tag number and Express Service Code number which are located on the side of the computer e Dell Hardware Limited Warranty Plus Onsite Service Extended Year e Dell Hardware Limited Warranty Plus Onsite Service Initial Year e Thank you choosing Dell ProSupport For tech support visit http support dell com ProSupport or call 1 866 516 3115 e ProSupport for IT Next Business Day Parts and Labor Onsite Response 2 Year Extended e ProSupport for IT Next Business Day Parts and Labor Onsite Response Initial Year e ProSupport for IT 7x24 Technical Support for certified IT Staff 2 Year Extended e ProSupport for IT 7x24 Technical Support for certified IT Staff Initial year E FLEXCELL INTERNATIONAL CORPORATION FEDERAL COMMUNICATIONS COMMISSION RADIO FREQUENCY INTERFERENCE STATEMENT This computing equipment has been type tested and found to comply with the limits for a Class B digital device in accordance with the specifications for Subpart J of Part 15 of FCC Rules These limits are designed to provide reasonable protection against harmful radio and TV interference in a residential installation There is no guarantee that interference will not occur in a particular installation The following instructions and precautions must be observed by the user when installing
52. uter connect the Ethernet cable from each FlexLink to an Ethernet Hub Then connect the hub to the Ethernet port on the back of the computer E FLEXCELL INTERNATIONAL CORPORATION Connect the system tubing as follows 1 Connect the 9 5 mm O D blue tubing from the pressure source to the SYSTEM port on the back of the FlexLink Fig 1 amp 2 2 Attach the free end of the clear 3 4 9 5 mm tubing to the FLEX OUT port on the back of the FlexLink Fig 2 3 Attach the free end of the blue 1 4 6 4 mm tubing to the FLEX IN port on the back of the FlexLink Fig 2 The VENT port should be left open NOTE When connecting the tubing to the back of the FlexLink slip the brass nut with the nylon ferrule over the tubing then push the tubing onto the fitting as far as possible and tread the nut onto the fitting Tighten each nut by hand until the tubing is completely crimped If the tubing is not firmly connected it may blow off under high pressures Connect the power cables 115 or 230 VAC to the rear power inlet FlexLink Power of the FlexLink Fig 2 If the available power supply is 115 VAC or 230 VAC be sure the voltage selection switch ON the computer Fig 3 is set to 115 V or 230 V respectively When the instruments are completely set up the power cords should be connected to a surge protected power strip and then to a 115 VAC or 230 VAC 60 Hz power outlet 2 4 INCUBATOR ACCESS
53. utlet strip Pop off valve Pressure gauge assembly FX 5000 COMPRESSION COMPUTER SPECIFICATIONS Dell OptiPlex Small Form Factor Intel Core i5 processor 3 2 GHz 250 GB SATA 6 0 Gb s hard drive DVD RW Drive 2 GB of DDR3 RAM Integrated video Intel HD2500 Operating System Windows 7 Professional FX5K COMPRESSION FLEXLINK COMPONENT SPECIFICATIONS Custom design positive pressure controller board e Microprocessor sampling rate for display data transducer 200 Hz e Microprocessor sampling rate for feedback error valve adjustment transducer 1 KHz 2 proportional solenoid valves Ethernet port RJ45 25 E FLEXCELL INTERNATIONAL CORPORATION FX5K COMPRESSION FLEXLINK CALIBRATION SPECIFICATIONS Static Performance Dynamic Performance Maximum allowable waveform variance from static line 0 15 Ibs Maximum force error actual vs programmed 0 35 Ibs Maximum allowable transducer inaccuracy 2 0 kPa Maximum force error actual vs programmed 0 35 lbs at the following Frequencies lt 10 lbs 0 5 and 1 0 Hz 10 lt x lt 14 lbs 0 5 Hz Maximum allowable transducer inaccuracy 2 0 kPa FX 5000 COMPRESSION PHYSICAL SPECIFICATIONS FX 5000 Computer Size W x Hx D Power Requirements Weight unit only FX5K Compression FlexLink Size W x Hx D Power Requirements Weight unit only Monitor 17 LCD Flat Panel Size Wx HxD Power Requirements Weight
54. within the foam ring Do this for the remainder of the wells Add 3 5 mL of culture medium to each well Prepare all stationary platens by adjusting the center screw Fig 7 such that the screw bottom part that touches the sample is exactly flush with the bottom of the platen body Use a pair of sterile forceps and the two holes at the adjustable center of the platen to turn the center screw Figure 7 Stationary platen and compression culture plate Place a stationary platen into each well of the compression plate Fig 7 Calculate the number of revolutions required to bring the bottom of the center screw to the point where it just touches the top of the sample by using the following equations For sample height in millimeters x 3 11 h 0 62 E FLEXCELL INTERNATIONAL CORPORATION where x is the of 180 degree clockwise turns required for the center screw to touch the sample and h is the sample height in millimeters For sample height in inches x 0 122 h 0 025 where x is the of 180 degree clockwise turns required for the center screw to touch the sample and h is sample height in inches NOTE If the sample height is larger than 3 11 mm 0 122 x in the above equations will be a negative number In this case the bottom of the center screw will compress the sample when it is flush with the bottom of the platen body as the platen is first inserted into the well To compensate for this
55. ximately centered on the gaskets i e not sitting on an area near the end of the gasket width Reseat plates as necessary and reclamp as before NOTE In many cases it may not be possible to get a perfect seal between the culture plates and gaskets When you are not able to reach your maximum requested force because of a baseplate leak problem try increasing your input pressure from your pressure source Be careful not to exceed 20psi Problem 2 Regimen wave is flatlining Solution 2 Remove the SYSTEM pressure line at the back of the controller and check to be sure that there is pressure coming through the line Check the VENT port at the back of the controller when the system is running to see if air is moving in or out If not it is possible that the valves are locked due to particle accumulation over time Try applying air pressure to the vent port to free the valves If the regimen wave is flatlining but the plates are still cycling air pressure try the following Disconnect the blue 1 4 6 4mm FLEX IN tubing from the back of the unit and place E FLEXCELL INTERNATIONAL CORPORATION your finger over the end of the tubing while a regimen is running If air is cycling at the end of the tubing then the problem is with the FlexLink Contact Flexcell International for further instructions Problem 3 Regimen wave is fluttering ot not smooth Solution 3 This problem is due to a miscalibration of the FlexLi
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