HTC PAL User Manual Edition2 1.5 MB mod. 2007-04-30
Contents
1. HTC PAL User Manual Printing Historv Edition 1 Mav 2000 Software Version 2 0 Edition 2 April 2003 Software Version 2 0 The PAL Svstem mav be operated with another level of firmware Details of the current level are given with the leaflet Firmware Overview which is placed in the front of this binder CTC Analytics AG reserves the right to make improvements and or changes to the product described at any time without notice CTC Analytics AG makes no warranty of any kind with regards to this product including but not limited to the implied warranties or merchantability and suitability for a particular purpose In no case shall CTC Analytics AG be liable for any coincidence ore consequential damages in connection with or arising from the use of this document 2003 CTC Analytics AG All rights reserved Neither this publication nor any part of this publication may be copied photocopied reproduced translated or reduced to any electronic medium or machine readable form without the prior written permission of CTC Analytics AG except as allowed under the copyright laws CTC Analytics AG acknowledges all trade names and trademarks used as the property of their respective owners Declaration of Conformitv General Considerations HTC PAL User Manual Safety Information See Declaration of Conformity sheet enclosed with the instrument A Changes or modifications to this unit not expressly approved by the party responsible2. 2 HTC PAL User Manual Figure 5 Attachment of Mounting Claws Installing the Injection Unit note Installation of the Injection Unit should be done carefully When installing it for the first time have someone hold it in place while the mounting screws are inserted see Figure 8 D Guiding Pins Figure 6 Attaching the PAL Injection Unit Remove the 3 Torx mounting screws A B and C that will be used to fix the Injection Unit to the Y Crossrail Place one of the screws onto the end of the supplied Torx driver Slide the clear plastic cover on the Injection Unit all the way to the top HTC PAL User Manual 2 Connect the flat ribbon cable A protruding from the front end of the Y Crossrail to the corresponding connector on the Injection Unit see Figure 7 A Guide plug into socket B Guiding pins into guiding holes 3 Hold the Injection Unit in place against the Y Crossrail Make sure the two locating pins on the Y Crossrail fit into the two guiding pin holes on the Injection Unit Figure 7 Connecting the Injection Unit Flat Cable 29 HTC PAL User Manual Locate the three large holes in the black anodized frame attached to the Z Crossrail inside the Injection Unit Slide the frame upwards until the top hole is centered on the top threaded hole in the end of the Y Crossrail Insert and securely tighten the Torx screw A see Figure 8 Install the two remaining Torx screws B C in the left an
3. Attach one end of the supplied transparent Tygon tubing to the Wash station waste port Place the other end of tubing into a waste reservoir not supplied which is lower positioned than the Wash station assembly Stretch the Tygon tubing to make sure that no used wash solvent is trapped before the Waste Reservoir The waste reservoir MUST be placed in a lower position than the Wash Station assembly Connect the control cable between WASHSTATION and the four pronged connector located on the Fast Wash Station assembly Figure 14 Electrical Connection Fast Wash Station 54 HTC PAL User Manual Installing a Microplate Stack If a Stack a Trayholder with multiple drawers was shipped with the HTC PAL loosen the two Torx screws on the two mounting clamps located on top of the Stack Figure 15 Installing a Microplate Stack Carefully lift the X Crossrail assembly on top of the stack with the mounting clamp teeth fitting into the grooves on the bottom of the X Crossrail Be sure that the damps fit completely into the grooves Alternately tighten the two Torx screws until the two mounting clamps are firmly in place Double check if the Stack clamps are correctly attached to the X Crossrail see Figure 5 Installing a Trayholder If a Trayholder was shipped with the HTC PAL loosen the two Torx screws on the two mounting clamps located on top of the Trayholder legs Figure 16 Installing a Trayholder 55 no
4. Update button to load the new firmware The process will take several minutes If the update is successfully completed the message Update of target memory succeeded will be displayed Click the Close button to exit the Backup dialog box Click the Start PAL button to terminate control by the Loader program On the Keypad terminal the Job Queue screen Is displayed Disconnect the RS 232 cable from the PAL 48 HTC PAL User Manual 12 Troubleshooting note l The HTC PAL represents always one of several components in a chromatography system The following Troubleshooting Guide is limited to the HTC PAL only Symptom or Error Message Possible Cause No or very low detector signal The valve needle guide and or needle seal are not Is observed properly installed Recommended Action Check valve needle guide and seal see chapter 9 2 Valve Needle Guide The valve ports are not plumbed correctiv to the pump and or detection svstem Check plumbing connections see chapter 9 1 Injection valve flow path Wrong valve tvpe specified Check valve type by pressing Menu Setup Objects Injectors LC Vlvi Valve Clogged syringe Remove syringe and aspirate dispense liquid manually Clean syringe Bent needle Inspect and or change syringe No sample liquid is injected Check and or adjust Needle penetration into sample vial see chapter 4 2 Sample volume too low Mobile phase
5. which hold the valve to the valve drive Check that the valve guiding pin remains at it s position Install the replacement valve in the reverse order Valve Rotor To replace and or clean the valve rotor follow the steps shown in Figure 34 Enlargement from the back Figure 33 Replacing Valve Rotor 52 HTC PAL User Manual 1 Do not remove the valve from the valve drive or any tubing or the loop 2 Unscrew the knurled pre load assembly Do not tamper with the preset socket adjustment screw 3 Engage the end of the rotor with a magnet or a pair of tweezers 4 Locate the letter e g H stamped on the new or cleaned rotor and orient the letter to the lower half of the valve 5 Insert the new rotor Make sure the rotor tab fits into the slot in the valve drive Replace the knurled pre load assembly 7 Tighten the pre load assembly It must end up fully bottomed out OD 13 3 Injection Unit Follow the sequence of steps shown in chapter 6 2 Assembling the HTC PAL 13 4 Wash Station Follow the sequence of steps shown in chapter 6 2 Assembling the HTC PAL 55 HTC PAL User Manual 14 Maintaining the PAL System Regularly performing maintenance helps ensure accuracy and precision of the PAL System Suggested intervals for maintenance procedures to ensure uninterrupted operation are given below If you use the system heavily for example nights and weekends or if you use aggressive solvents you may need to pe
6. OFF or more than one power cord has to be pulled from power supply or from the wall outlet to shut down the complete PAL System If the basic PAL System is installed than a single power supply is installed only Turning OFF the power supply or pulling this single power cord in an emergency case will stop the complete PAL System It is important that the power supply ies are in a location where the power ON and OFF switch is accessible and easy to operate and where it is possible to unplug the AC power cord from the power supply wall outlet in case of emergency Other Hazards HTC PAL User Manual A To avoid injury during PAL System operation keep your hands away from the syringe A Do not operate the PAL System without the safety shield The safety shield must be installed for safe operation To avoid injury observe safe laboratory practice when you handle solvents change tubing or operate the PAL System Know the physical and chemical properties of the solvents you use See the Material Safety Sheets from the manufacturer for the solvents in use A Use caution when working with any polymer tubing under pressure e Always wear eye protection when near pressurized polymer tubing e Do not use polymer tubing that has been severely stressed or kinked e Do not use polymer tubing in particular not PEEK or Tefzel tubing with Tetrahydrofuran THF Dimethylsulfoxid DMSO chlorinated organic solvents concentrated miner
7. Object e g LC VIV1 Travi or Stack1 01 Injection valve drive not connected Insert sample vial Deepwell or Microplate Redefine Z value for the affected Object see chapter 7 1 Defining Object positions Switch OFF PAL and check connection between PAL and LC Viv see chapter 6 3 Defective LC Vlv1 Connection cable Change connection cable Control Board defective Replace Control Board see chapter 15 1 Control Board 50 HTC PAL User Manual 13 Replacing Parts 13 1 Control Board 1 Follow the sequence shown in Figure 31 to detach and release the Control board from its position inside the X Crossrail 2 Carefully pull the two flat cable connectors upwards to free from the board 3 Install the replacement board in the reverse order note After reconnecting the two flat cables to the board ensure that the cables are folded flat and can be slid inside the cross rail without damage LI POWER LED Buzzen SERS SER2 SER1 c lil Lit ee a O 0 0 0 INTERFACE was e oo E o o e Figure 31 Replacing Control Board 5 gt UNN HTC PAL User Manual 13 2 Injection Valve and Valve Rotor Injection valve To replace and or clean the injection valve follow the steps shown in Figure 32 Figure 32 Replacing the Injection Valve Remove the sample loop needle guide fitting and connection tubes from the valve body Unscrew to the hex screws
8. PAL User Manual 4 4 Wash Station After selecting the specific Wash Station the following Functions are available Function Key Description F2 Act Valve F2 switches the selected Wash Station valve between the Open and Close position It may be used to check the valve function or to prime the wash solvent lines F4 HOME The Injection Unit moves to its HOME position and the Job Queue Menu is displayed The following Wash Station Parameter may be changed by selecting the particular item Item Description Rinse Time Rinse Time may be used to expand the solvent flow time through the wash station glass liners after the last wash stroke is performed and the needle is retracted If Rinse Time is set to 0 the wash station valve is closed immediately after the last wash stroke 21 HTC PAL User Manual B HTCPAL Description and Installation 5 General Svstem Overview T H L Injection unit Keypad Terminal Syringe and Y Crossrail syringe adapter Plunger holder X Crossrail Safety guard Microplate stack Trayholder Tray Fast Wash Station LC Injection Valve Figure 4 HTC PAL Major System Components The basic HTC PAL is equipped with a 6 port LC Injection Valve and a Fast Wash Station 1 or 2 microplate stacks or trayholders may be added to complete the system Optional cooled Trayholdes and Stacks and 4 or 10 port Injection Valves are available and can be added at
9. Recommended practice Is to create the following directory structure to store PAL firmware updates and backups Directory Path Contents C ProgramFiles PAL Loader Backup Full backup files of current and previous PAL configurations includes firmware and parameter settings maintained for archival purposes C ProgramFiles PAL Loader Update Extracted Update files in Loader format sss may include firmware only or firmware and parameter settings Shut off power to the PAL and the computer Connect the supplied gray cable between the PAL SER1 and a PC serial port Switch on power to the PAL When the PAL Job Menu is displayed start up the computer On the PC desktop click the PAL Loader icon the PAL Loader screen appears PAL Loader ji Info Update Backup Setup Start Pal i Figure 29 PAL Loader menu screen Click the Backup button A dialog box entitled Backup Target Memory will be displayed The path storage location and file name to be assigned to the new backup will be shown A customized path and or filename may be entered B ackup Target Memory Filename Browse CAPROGRAMMENPALNLOADERNBACKUPABK 380825 Backup Log ii oe Backup pam 4 Figure 30 PAL Loader Backup Target Memory 4 note l HTC PAL User Manual Click the Backup button Backup will proceed and information on the backed up components will be displayed in the Backup Log window The pro
10. Z Positions by pressing F1 Check Pos 58 HTC PAL User Manual 7 2 Description of Object Reference Positions Trayholder e g THldr1 Figure 20 Trayholder Reference Position For Trayholder the Reference Position is a hole in the base plate of the Holder The lower needle guide should be centered in the hole with the bottom of the needle guide flush with the bottom of the base plate Trayholder with six or three drawers e g Stack1 Figure 21 Stack Reference Position For a six drawer Stack the reference position is a hole located in the second drawer see Figure 21 For a three drawer Stack the reference position is in the first top drawer see Figure 21 The lower needle guide must be centered in the hole with the bottom of the needle guide flush with the bottom of the drawer 39 note l note l HTC PAL User Manual Figure 22 Fast Wash Station Reference Positions For a Fast Wash Station the reference positions are two holes above the Wash Station glass liners see Figure 22 Wash and Wash2 The lower needle guide should be centered in these holes with the bottom of the lower needle guide lightly touching the surface of the Wash Station assembly The Waste position represents an Injector within the PAL software It is defined in the Object class Injectors see Fig 23 Injectors e g LC Vlv1 or Waste Figure 23 LC Valve and Waste Reference Position For an LC Valve the reference pos
11. after the syringe needle is moved out of the sample liquid Pre injection syringe wash strokes with solvent from Wash1 Pre injection syringe wash strokes with solvent from Wash2 Pre injection syringe rinse cycles with sample Speed of plunger movement used to aspirate sample Number of filling strokes to aspirate sample Delay time between sample pull up and ejection Name of Injector used for injection Speed of plunger movement used during sample injection Delay time prior sample injection Delay time after sample injection Post injection syringe wash strokes with solvent from Wash Post injection syringe wash strokes with solvent from Wash2 Valve rinse cycles with solvent from Wash Valve rinse cycles with solvent form Wash2 59 YU0ISNAY c ro qp e D T QO KO T ad ABI mauad APAN US up AIA Mi LAS UL AI tk ZNS UD ISd adj Ap1 IAS UD Bd mUd IJPIAN palu Ed 1 uj ald Laut aie DAS ati BS UY ad Ae1 0 palu 5 Juno weap npud IPN pa dnjng auinpA WED jw SOMOS 1jguag apaaN paads Il O TEEM adila paads poluj ids up aug LL spuoas Mead paN paads pala INS UD ald sainuiy Rana es ZLIW LOLW paads i4 INS UD 34d i SINOH qor pug ONS Wa 5A5 0 WeD ja dnd auunjo Aly co uuo jo feq apA pug hay dns Bwen wasAs SIA ESL al Ae SO OJS II auunjo adwes Uu a0 UEM Aay pote JWEN aus Ijguad IPWN Jue APAN auinpo Ild JONMAS F A xog 38esso y Aay
12. for compliance could void the user s authority to operate the equipment The user shall be made aware that if the equipment Is used In a manner not specified by the manufacturer the protection provided by the equipment my be impaired When you use the PAL System follow the generally accepted procedures for quality control and methods development When you use the PAL System in the field of chromatographic analysis and you observe a change in the retention of a particular compound in the resolution between two compounds or in peak shape immediately determine the reason for the changes Until you determine the cause of a change do not rely on the separation results Electrical Hazards HTC PAL User Manual Every analytical instrument has specific hazards so be sure to read and comply with the following precautions They will help ensure the safe long term use of your PAL System The Installation Category Over voltage Category for this instrument is Level Il The Level Il Category pertains to equipment that receives its electrical power from the local level such as an electrical wall outlet Only use fuses of the type and current rating specified Do not use repaired fuses and do not short circuit the fuse holder LN The supplied power cord must be inserted into a power outlet with a protective earth contact ground When using an extension cord make sure that the cord also has an earth contact A Do not change the e
13. from electrical shock Class identifies a single level of insulation between live parts wires and exposed conductive parts metal panels in which the exposed conductive parts are connected to a grounding system In turn this grounding system Is connected to the third pin ground pin on the electrical power plug b Over Voltage Category Il Over Voltage category Il pertains to instruments that receive their electrical power from a local level such as an electrical wall outlet c Pollution Degree2 This is a measure of pollution on electrical circuits that may produce a reduction of the dielectric strength or surface resistivity Degree 2 refers to normally only non conductive pollution Occasionally however a temporary conductivity caused by condensation must be expected d Moisture Protection Normal IPXO IPXO means that there is NO Ingress Protection against any type of dripping or sprayed water The X is a place holder to identify protection against dust if applicable 24 HTC PAL User Manual Electrical Specifications Continued Parameter Requirements 4 w we Z PAL System nj PAL System Power Supply A Me ee PAL Single Valve Module a PAL 3 Valve Module a PAL 4 Valve Module a Input Voltage A N PAL Valve Module Power Supply serial control Grounded AC 100 t0 240V __ Grounded AC 100 to 240 V a TAE Stack Cooler DW MT or Tray Cooler MEE Peltier Element o CT Tga Power Su
14. of filling strokes in a HTC PAL Method Gas tight syringes must be primed manually Remove the plunger and fill the syringe from the top of the barrel until solvent either overflows or forms a bead at the top of the syringe Then insert the plunger Plunger Holder Figure 24 Syringe and Syringe adapter 4 note l 8 3 HTC PAL User Manual Installing a Syringe Select Menu and press F1 Chang Syr The Injection Unit will move to a location that will facilitate installation of the syringe Place the syringe in the appropriate syringe adapter Pull the plunger out to approximately 20 of it s length If necessary loosen the plunger retaining screw in the plunger holder see Figure 25 Move the syringe installed in the syringe adapter partially into the Injection Unit Guide the needle first into the upper needle guide and then into the lower needle guide Place the plunger button into the plunger holder Allow the syringe adapter to dick into place by magnetic force against the syringe carrier Tighten the plunger retaining screw against the plunger button Press Home The plunger moves down until it hits the mechanical stop This position is stored as the syringes zero volume position Then the Injection Unit returns to the HOME position After replacing a syringe always check the needle penetration in the LC valve see chapter 9 5 page 36 Injection Valve Needle Penetration E Figure 25 Install
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16. 0 ml 6 Trays may be used in Deep well Stack PAL Stack6DW 56 HTC PAL User Manual C Injection Cvcles HTC PAL LC Inj Cycle No Processing Step Method Parameter Object Class Used Object Value Reference X Y Z axis and syringe System Start Ref ON plunger 2 Wait for Start signal Start Sync Signals 3 Read Barcode if enabled TRAY Tray Type Barcode None 4 Syringe wash strokes with solvent Pre Cln Slv1 SYRINGE Syringes Fill Speed from Wash Eject Speed Wash Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 5 Syringe wash strokes with solvent Pre Cln Slv2 SYRINGE Syringes Fill Speed from Wash2 Eject Speed Wash2 Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 6 Rinse syringe with sample selected Pre Cln Spl SYRINGE Syringes Eject Speed number of times Sample Volume Fill Volume Fill Speed TRAY Tray Type Needle Penetr Pullup Delay Waste Injectors Needle Penetr 7 Fill syringe with sample using Sample Volume SYRINGE Syringes Eject Speed several filling strokes aspirate air Air Volume Overfill outside of vial if Air Volume 7 0 Fill Speed Fill Volume Fill Strokes TRAY Tray Type Needle Penetr Pullup Del 8 Move to injection valve Inject to 9 Wait for Inject signal Inject Sync Signals 10 Drive needle to penetration depth Inject to Injectors Needle Penetr 11 Delay Pre Inj Del 12 Switch va
17. 8 Figure 9 Figure 10 Figure 11 Figure 12 Figure 13 Figure 14 Figure 15 Figure 16 Figure 17 Figure 18 Figure 19 Figure 20 Figure 21 Figure 22 Figure 23 Figure 24 Figure 25 Figure 26 Figure 27 Figure 28 Figure 29 Figure 30 Figure 31 Figure 32 Figure 33 PAL Control Terminal and Conventions Accessing a Method Screen Example Job Queue Screen HTC PAL Major System Components Attachment of Mounting Claws Attaching the PAL Injection Unit Connecting the Injection Unit Flat Cable Inserting the Injection Unit Mounting Torx Screws HTC PAL with Injection Unit and Standalone Supports Installing Keypad Terminal Installing the Injection Valve Drive Installing the Fast Wash Station Fast Wash Station Assembly Electrical Connection Fast Wash Station Installing a Microplate Stack Installing a Trayholder HTC PAL Electrical Connections Object Reference Position Menu Screen Object Trayholder Trayholder Reference Position Stack Reference Position Fast Wash Station Reference Positions LC Valve and Waste Reference Position Syringe and Syringe Adapter Installing and Removing a Syringe LC Injection Valve Flow Path Valve Needle Guide and Valve Needle Seal Menu Screen Valve Needle Penetration Depth PAL Loader Menu Screen PAL Loader Backup Target Memory Replacing Control Board Replacing Injection Valve Replacing Valve Rotor HTC PAL User Manual note l HTC PAL User Manual How to Use this Manual The manual is di
18. HTC PAL Objects j D d a A A jr ue i lU j 3 a a A Fig 18 Object Reference Position For Trayholder the Reference Position is a hole slightly larger than the lower needle guide in the base plate of the Holder The lower needle guide should be centered in the hole with the bottom of the needle guide flush with the bottom of the base plate Switch ON the HTC PAL power supply When the Job Queue menu is displayed complete the following sequence common to all objects Menu Setup Objects Tray Holders Named Tray Holder where Named Tray Holder represents a predefined Trayholder e g THldr1 or Stack1 57 HTC PAL User Manual Tray Holder Position X Position Y Position 4 Access Path 1998 08 31 11 80 85 Check Clear Pos Pos Pos Figure 19 Menu Screen Object Trayholder After selecting Named Tray Holder e g THldr1 or Stack1 the X Y Z positions for the selected object will be displayed see Figure 19 Highlight the item Position X with the cursor bars and press ENTER The Injection Unit will move to the previous defined X axis position Rotate the outer knob to adjust the X axis position to the Trayholder Reference Position see Figure18 page 28 Press the inner knob to ENTER the Position X value Repeat steps 3 5 for Position Y and Position Z Press the F3 button Moveto Zero The Injection Unit will move to the HOME position Verify the defined X Y
19. al acids such as Nitric Phosphoric or Sulfuric acids or any related compounds to above listings Lithium batterv HTC PAL User Manual An onboard lithium battery buffers the electronic memories when the instrument Is turned off Replace it only with the same or equivalent type recommended by the equipment manufacturer Battery Panasonic VL 2330 soldered directly on the electronic board Discharged lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries There are no operator serviceable parts on the electronic boards If an electronic board fails contact a CTC Analytics representative HTC PAL User Manual Commonly Used Symbols Electrical Power ON Used with Main PAL Power Supply Electrical Power OFF Used with Main PAL Power Supply Electrical Power ON for Only Part of the System Used with Optional Device s Electrical Power OFF for Only Part of the System Used with Optional Device s Caution Risk of Electrical shock high voltage HTC PAL Operating Manual General Information Printing History Satetv Information Tables of Content HTC PAL Operating Instructions Using the Control Terminal Menu Screens Function Kevs ESCape and STOP Kevs Scroll Knob and ENTER Button Methods Creating Methods Edit View Methods Delete Methods Job and Job Queue Building and Starting a Job Queue Aborting a Job Queue Restarting an Aborted Job Qu
20. and or sample The valve rotor is reversed 180 out of alignment flows out of the wrong ports on the LC injection valve Sample is backing up on the Syringe needle OD too small valve needle guide Increase sample volume Remove the rotor and reinstall in the correct position Look for a letter e g H stamped on the rotor The letter must be on the lower half of the rotor see chapter 13 3 Valve Rotor Check the syringe needle for gauge 22 Valve needle seal leaks Change Valve needle seal see chapter 9 2 Valve needle guide The needle penetration depth for the injection valve is not set correctly Adjust the Injection Valve Needle Penetration see chapter 9 5 The Syringe Plunger Speed is too high resulting in excessive pressure in inlet Poor reproducibility Syringe pressure differences when filling the syringe Reduce Inject Speed in method Increase Pullup Delay value Vacuum created in sample vial Reduce sample volume in sample vial High volatile solvent Use gastight syringe and sample tray cooling Increase Pullup Delay value Reduce Filling Speed in method Not properly crimped vials Excessive carryover between Dirty syringe and or valve injection port samples Check vial cap by attempting to rotate by hand Loose caps may cause selective loss of lighter components from sample Adjust crimping tool correctly Increase Pst Inj Slv1 and or Viv Cln Slv1 va
21. any time 22 Sample capacitv Thermostatted Sample Travs Thermostatted Stack Injection volume range Replicate injections Wash Station Precision Carryover Injection cycle time 5 1 HTC PAL User Manual Specifications 98 x 2ml vials per Tray 200 x Iml vials per Tray 32 x 10ml 20ml vials per Tray up to 2 Trays may be configured 6pcs Deepwell or Standard Microplates in a 3 drawer Stack 12pcs Standard Microplates in a 6 drawer Stack 6pcs 54 position 2ml Vial Trays in a 3 drawer Stack up to 2 Stacks may be configured Optional up to 2 thermostatted Trayholders 4 C 70 C Optional 1 thermostatted 3 drawer Stack 4 C 70 C 10 100ul with standard 100ul syringe Down to 0 5ulwith solvent sandwich injection Down to 0 5ul with optional 4 port internal loop configuration up to 5000ul with optional larger loop and syringe 1 99 from one vial 2 different wash solvents Typically lt 0 5 RSD of peak area from 10ul 100 Typically lt 1 0 RSD of peak area from lt 10ul Typically lt 0 1 Typically 20 60 sec depending on plunger speeds injection volume and wash cycles 25 HTC PAL User Manual Electrical Specifications Parameter Protection Class Class Over Voltage Category Category ll Pollution Degree 2 Moisture Protection Normal IPXO a Protection Class l Protection class describes the insulating scheme used in the instrument to protect the user
22. bench and remove the foam pieces from each end Unpack the remaining small boxes and any other containers Place the HTC PAL onto a stable surface Make sure that no objects will interfere with either the Y Crossrail or Injection Unit throughout the entire range of potential movements Assembling the HTC PAL Before beginning the assembly process determine approximately where the LC injection valve and keypad terminal will be located The terminal can be mounted on either side of the X Crossrall If a Stack a Trayholder with multiple drawers was shipped with the HTC PAL loosen the Torx screws on the two mounting clamps located on top of the stack Move the Y Crossrail to the center of the X Crossrail and temporarily place the X Crossrail assembly on top of the stack with the mounting clamp teeth fitting into the grooves on the bottom of the X Crossrail The stack should be near the center of the Crossrail For HTC PAL s without a Stack support the Crossrail in a suitable manner a sturdy cardboard box can be used before attempting to install the legs Install the legs near the ends of the X Crossrail Loosen the Torx screws on the mounting clamps and then fit one leg into the grooves in the X Crossrail Be sure that the clamps fit completely into the grooves Tighten the Torx screws until the legs are firmly in place Double check if the legs and stack claws are correctly attached to the X Crossrail see Figure 5 following page
23. cess will take several minutes The message Backup of Target Memory succeeded indicates a successful backup Click the Close button to exit the Backup dialog box Click the Start PAL button to terminate control by the Loader program On the Keypad terminal the Job Queue screen Is displayed Disconnect the RS 232 cable from the PAL 11 2 Reloading a Backup This operation can potentially overwrite existing parameter settings Methods created by the user will be lost The firmware file to be loaded must reside on the PC hard drive The file cannot be loaded from a floppy drive A full update including parameter settings will not fit on a single floppy diskette in the PAL Loader format It must be transferred to the PC hard drive in compressed zip format and extracted into the sss file which is the PAL Loader format Complete the following steps to reload a PAL backup Shut off power to the PAL and the computer Connect the brown 9 pin cable between the PAL SERT and a PC serial port Switch on power to the PAL When the PAL Job Menu is displayed start up the computer On the PC desktop double click the PAL Loader icon the PAL Loader screen appears Click the Update button Click the Browse button and select the appropriate sss backup file Click the Open button The Update Flash Memory window will be displayed The path and filename for the update file will be shown in the Filename entry box Click the
24. d right mounting holes respectively It may be necessary to move the elastic cord slightly to the left to insert the Torx screw C into the right hand hole Figure 9 HTC PAL with Injection Unit and Standalone Supports 50 note l HTC PAL User Manual if Ye B Doo RF hhmgfl l t Figure 10 Installing Keypad Terminal Install the Safety Shield on the left and right sides to the outside of the X Axis Use the provided longer thumbscrew on the side where vou plan to install the kevpad Install the Keypad mounting bracket on either the right or left side of the X Crossrail Connect one end of the white coiled cable Part SS8J 700 to the Keypad and the other end to the SER3 interface jack on the rear side of the X Crossrail Place the Keypad terminal onto it s mounting bracket Installing the Power Supply Locate the power supply the DC power cable Part RS3M 2000 and the AC power cable Set the power supply switch to the OFF position Connect one end of the DC power cable to the power supply and the other end to the POWER connector at the rear side of the X Crossrail Connect the female end of the AC power cable to the power supply Then connect the male end to an AC power outlet Set the switch on the power supply to the ON position Observe the Keypad display The model name HTC PAL will be displayed along with the software version number The Job Queue menu screen will then be dis
25. down stepwise into the Injection port When the syringe needle tip enters the valve needle guide slow down the Z movement again Always observe the syringe needle during this operation step Move single step wise down until you notice a click sound inside the Injection Unit STOP immediately the Z down movement Rotate the outer knob two steps in the opposite direction and press ENTER to save the Needle Penetration Depth value Press F3 Movto Zero Verify the defined Needle Penetration Depth value by repeating Step 2 and 3 After all positions have been set and the Valve Needle Penetration has been adjusted perform a PC backup of the existing configuration see Chapter 11 PAL Loader Program 45 Menu HTC PAL User Manual 10 Interfacing the HTC PAL to Other Devices 10 1 Svnchronization and Output Signals Synchronization Signals Sync Signals are inputs that tell the HTC PAL when to wait or proceed with a sample processing step Output Signals Out Signals are sent from the PAL to external devices to indicate status or completion of particular processing steps These signals are classified as Objects Physical Events e g TTL In1 are also Objects and may be assigned to named signals PAL Cycles require that certain signals such as Start Inject and Injected be defined See Table below for the PAL Events and signal assignments associated with the standard PAL LC Inj Cycle To assign a physical Event e g SW Ou
26. e Add a new Job for the Tray Press the Add Job key to bring up the default Job For TRAY select the Tray Name e g Stk1 01 that corresponds to the location of the Tray that was just loaded Enter the First and Last sample number for this Job Select and enter the sample processing Method for this Job Press the Home function key F4 to return to the JOB QUEUE screen To add additional samples to be processed repeat steps 2 7 If necessary replace and or clean the Syringe Press the Menu key to see the available options for changing F1 Change Syringe and cleaning F2 Clean Syringe To completely remove air bubbles the Syringe should be primed manually see chapter 8 2 page 32 Syringe priming Close all Stack drawers 1 52 3 3 HTC PAL User Manual If only one Job will be processed select that Job with the scroll knob Press Start key In the dialog box Select Job s to Process select one of the following options All Entire Job Queue starting from the top Selected Job selected with the cursor bars Resume Continue with the next Job after the aborted one Aborting a Job Queue Press STOP Select one of the available options Continue Sample Job or Job Queue Select Continue to resume processing with the current sample Select Sample to abort processing of the current sample Processing will resume with the next sample Select Job to abort processing of all samples in the curre
27. elected This feature is especially useful for handling viscous fluids or syringe sizes 2100 Speed of plunger movement used in all syringe filling operations Speed of plunger movement used in all syringe eject operations except sample injection Speed of plunger movement for sample injection Plunger position during Chang Syringe operation The syringe plunger is moved to a position where the syringe can be removed and replaced The value may be changed for different types of syringes HTC PAL User Manual 4 2 Tray After selecting the particular Tray to be accessed the following Functions are available Function Key Description F3 Movto nnn This function serves as a quick check to determine if the XYZ coordinates are defined correctly for the selected Tray To use this utility the selected Tray including the sample vials or Microplate must be present After pressing Movto 001 the Injection Unit moves to sample position no 1 This procedure can be repeated for the last sample position in the first row and the last sample position in the last row F4 HOME The Injection Unit moves to its HOME position and the Job Queue Menu is displayed The following Tray Parameters may be changed by selecting the particular item Item Description Needle Penetr Needle penetration depth into the sample vial The stored default values for each Sample Tray Type are approx 2mm above the sample vial Microplate bottom The needle pe
28. eue Utility Functions Syringe Tray Injector Wash Station HTC PAL Description and Installation General System Overview Specifications Installation Unpacking the Components Assembling the HTC PAL Electrical Connections HTC PAL Object Positions Defining Object Reference Positions Description of Object Reference Positions HTC PAL User Manual O 13 14 14 14 14 15 15 16 16 17 17 18 18 19 20 20 2 22 22 25 2 27 27 56 57 57 59 8 Syringes 8 1 Selecting Syringes 8 Svringe Priming 8 5 Installing a Syringe 8 4 Removing a Syringe 9 Injection Valve 9 1 Injection Valve Flow Path 9 2 Valve Needle Guide 9 5 Injection Valve Needle Penetration 10 Interfacing the HTC PAL to other Devices 10 1 Synchronization and Output Signals 11 PAL Loader Program 11 1 Backing up the PAL Control Program 11 2 Reloading a Backup 12 Troubleshooting 13 Replacing Parts 13 1 Control Board 15 2 Injection Valve Valve Rotor 15 3 Injection Unit 15 4 Fast Wash Station 14 Maintaining the PAL System Appendices A Definition of Terms B Conventions of Naming C HICPAL Injection Cycle D Software Flow Chart E External Connectors HTC PAL User Manual 4 4 4 42 42 45 45 44 45 46 46 4 4 48 49 5 5 52 90 54 55 99 56 5 60 62 List of Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure
29. ing and Removing a Syringe Removing a Syringe Select Menu and press F1 Chang Syr The Injection Unit will move to a location that will facilitate removal of the syringe Loosen the plunger retaining screw Move the plunger slightly out of the plunger holder Pull the syringe adapter out and then carefully up to remove the syringe adapter with the syringe from the Injection Unit 42 HTC PAL User Manual 9 Injection valve 9 1 Injection Valve Flow Path A HTC PAL is equipped with 1 LC Injection valve The standard LC injection valve is a VICI VALCO Type W 6 port valve The 6 port valve is connected to the detection system according the flow path showed in Figure 26 Other avallable LC Injection valves are 4 port valves with internal loop and 10 port valves Sample in Carrier mobile phase Figure 26 LC Injection Valve Flow Path 45 note l HTC PAL User Manual 9 2 Valve Needle Guide All valves are equipped with a special valve needle guide fitting Part MV 30 12A This fitting has a wide diameter to mate with the syringe needle guide on the injection unit The valve needle guide also has a countersunk hole to facilitate insertion of the syringe needle into the injection port Valve Needle Guide Valve Needle Seal Figure 27 Valve Needle Guide and Valve Needle Seal The valve needle guide holds the needle seal The needle seal which is a short length of Teflon tubing forms the seal around
30. ions for that item or to change a numeric value Then press the inner knob again to ENTER the displayed option The inner knob is also used for other operations that require an ENTER operation to continue or complete an operation note l HTC PAL User Manual 2 Methods 2 1 Creating Methods Methods can be defined by the user and assigned names up to eight characters in length Methods can be created copied edited and viewed from the Method menu Methods can be viewed but not edited from the Job menu Methods are created by either copying an existing Method or creating a new Method To copy a Method complete the following sequence 1 Menu Methods Method name Copy Method 2 You will be prompted to enter a name for the new Method Use the scroll knob and the left right arrow function keys F2 and F3 to select among alphanumeric characters and spaces Press the ENTER function key F4 to accept the name To create a new Method complete the following steps l Menu Methods Select the insertion point Insrt Method 2 Assign and enter a new Method name as above After a copy of the Method has been created the Method parameters will be displayed and can be edited The Cycle and Syringe entries cannot be changed 5 If the Method is new i e added select and enter a Cycle that is appropriate for the application 4 Select the specific Syringe to be used by the Method Once a Method has been created and saved the Cycle and Syringe can n
31. ition is the valve needle guide fitting mounted on the top valve port see Figure 23 The lower needle guide of the Injection Unit should be centered In the valve needle guide fitting Adjust the Z Position that the bottom of the lower needle guide Is just touching the surface of the valve needle guide fitting Then reduce the value by 2 0 mm For the Waste Port the reference position Is a hole slightly larger than the needle guide in front of Wash2 see Figure 23 The lower needle guide should be centered in this hole with the bottom of the lower needle guide lightly touching the surface of the Wash Station assembly To adjust the Valve Needle Penetration value see chapter 9 3 page 36 40 HTC PAL User Manual 8 Syringes 8 1 Selecting Syringes The HTC PAL is shipped with 1 PAL SyrLC100ul Kit LC Syringe 100ul for HTC PAL consisting of pc syringe adapter 1 pc plunger holder 2 pc syringe SYRC G100 22S 3 The SYRC G100 22S 3 Syringe gastight 100ul for HTC PAL equipped with 22 gauge needles is appropriate for injection into the standard CTC injection valve However certain applications may require different syringe sizes Consult the PAL Accessories guide section HTS PAL in Appendix G 8 2 Syringe Priming It is critical that syringes be primed before beginning sample preparation Prime the syringe by using the Utility function Clean Syr For fixed needle syringes priming may also be achieved by using an adequate number
32. lues in method Use PreClnSlv1 and PreCInSpl Waste tubing I D at Injection valve is too small causing waste liquid to be pulled back by capillary action into the rotor groove Replace the waste tubing with larger I D tubing Damaged or grooved valve rotor Replace valve rotor see chapter 15 5 Valve Rotor Valve needle seal leaks Inappropriate wash solvent Unexplained chromatographic Air is being introduced into the injection loop Needle peaks penetration depths may be inadequate causing air to be pulled into syringe Change Valve needle seal see chapter 9 2 Valve needle Guide Use appropriate wash solvent Check and or adjust Needle Penetration into sample vial see chapter 42 Make sure the Air Volume Method parameter is set to zero 49 Unexplained collisions of the Injection Unit Error Message Object Not Detected Object e g Travi LCVIvI Stk1 01 Error Message Object Collision before Z Tolerance Object e g Travi LCVIv1 Stk1 01 Error Message Motor AUX failed HTC PAL User Manual Object s not defined correctly Incorrect Z value for the affected Object e g LC Vlv1 Travi or Stack1 01 Define Object s correctiv see chapter 7 1 Defining Object Positions Redefine Z value for the affected Object see chapter 7 1 Defining Object positions Missing sample vial Deepwell or Microplate Incorrect Z value for the affected
33. lve to Active position 13 Activate Injected signal Injected Out Signals Pulse Time 14 Activate Injectd Signal Injectd Out Signals Pulse Time 15 Dispense syringe contents into Inject Speed sample loop 16 Delay Pst Inj Del 17 Switch valve to Standby position 18 Retract needle from injector 19 Clean syringe and needle with Pst Cln Slv1 SYRINGE Syringes Fill Speed solvent from Wash1 Eject Speed Wash Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 5 HTC PAL User Manual 20 Clean syringe and needle with solvent from Wash2 SYRINGE Syringes Fill Speed Eject Speed Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 21 Rinse valve inlet with solvent from Wash1 SYRINGE Syringes Fill Speed Inject Speed Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 22 Rinse valve inlet with solvent from Wash2 SYRINGE Syringes Fill Speed Inject Speed Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 25 Move to Home position 50 HTC PAL User Manual LC Inj Cycle Parameter Description Parameter YCLE SYRINGE Sample Volume Air Volume Pre Cln Slv1 Pre Cln Slv2 Pre Cln Spl Fill Speed Fill Strokes Pullup Del Inject to Inject Speed Pre Inj Del Pst Inj Del Pst Cln Slv1 Pst Cln Slv2 Viv Cln Slv1 Viv Cln Slv2 Description LC Inj Cycle Selected syringe Selected sample volume Volume aspirated
34. netration depth for the selected Tray may be changed by entering the desired value Tray Type The Tray Type which is selected for the Tray is shown If the Tray enables use of different Tray Types it can be changed here Alternate use of Microtiter or Deepwell plates in a Stack 4 5 Injector After selecting the particular Injector to be accessed the following Functions are available Function Key Description FI Rinse Inj After pressing F1 either Wash1 or Wash2 may be used to clean the LC injection valve port without running a Job F2 Act Valve F2 switches the LC injection valve between the Load and Inject position It may be used to test the valve drive function or to perform manual injections F3 Movto In The Injection Unit moves to the injection valve position Bv selecting the Parameter Needle Penetr on the same screen the Injection Valve Needle Penetration value mav be checked or changed see chapter 9 3 F4 HOME The Injection Unit moves to its HOME position and the Job Queue Menu is displaved The following Injector Parameter may be changed by selecting the particular item Item Description Needle Penetr By selecting the Parameter Needle Penetr the Injection Valve Needle Penetration value may be checked and or changed To ensure reproducible sample injection and minimize carryover it is critical that the needle penetration depth is set accurately see chapter 9 3 Injection Valve Needle Penetration 20 HTC
35. nt Job Processing will resume with the next Job The aborted Job is marked with an X Select Job Queue to abort processing of all Jobs The JOB QUEUE screen will be displayed The aborted Job is marked with an X Restarting an Aborted Job Queue Press the START key Select the option Resume The job after the last one marked as aborted will be started note l 4 1 HTC PAL User Manual Utility Functions Utility functions selectable from the Menu screen provide quick access to checking operations and parameters that may need to be changed These functions are available for the actual Syringe Trays Injectors and Wash Stations They allow access to key functions without having to set up and execute a Method and Job If an item is used in the sample processing cycle the appropriate Utility value will be overwritten by the Method value Syringes By pressing a Function Key the following functions are available Function Key F1 Chang Syr F2 Clean Syr F3 Set Pos F4 HOME Description The syringe is moved to a position where the syringe assembly can be completely lowered to facilitate removal of the syringe adapter The syringe can then be removed and replaced A prompt will be displayed to specify the new syringe The syringe must be installed before pressing Enter see Chapter 8 Syringes This Function is used to clean or prime the syringe prior use After selecting F2 either Wash1 or Wash2 can be selec
36. on of Terms A Job Queue is a list of sample processing Jobs Jobs are executed in the order displayed on the JOB QUEUE menu screens New Jobs may be added to the queue while samples are being processed A Job contains the information needed by the PAL to process multiple samples by the same processing steps The elements of a Job are a Method and a Tray that defines the location of the samples to be processed For identification Jobs are automatically numbered from 01 to 99 and then restarting with 01 when they are added to the Job Queue A Cycle consist of the specific operations necessary to process one sample The Cycle operations are repeated for each sample within a Job Cycles are designed for specific applications The Standard HTC PAL Cycle is named LC Inj A Method defines how the samples are processed The elements of a Method are a Cycle a Syringe and a Parameter List Methods have names with up to 8 characters and can be edited copied and deleted Method Parameters are associated with the Cycle operations User assigned Parameter values define how a processing operation is performed A zero Parameter value will disable a Cycle operation Cycle parameters are application specific Objects are data structures describing the properties of physical modules Certain modules e g a Stack require several objects A Travholder can hold one or more trays Each Trayholder has a reference position X Y Z co ordinates that defines i
37. orable conditions e g subjected to heat water etc In any case disconnect the power cord s from the power supply or from the different power supplies if optional devices are installed before attempting any type of maintenance Capacitors inside the instrument may still be charged even if the instrument is turned off A To avoid damaging electrical parts do not disconnect an electrical assembly while power is applied to the PAL system Once the power is turned off wait approximately 30 seconds before you disconnect an assembly The instrument includes a number of integrated circuits These circuits may be damaged if exposed to excessive line voltage fluctuations and or power surges HTC PAL User Manual A Never try to repair or replace any components of the instrument that is not described in this manual without the assistance of a CTC Analytics representative There are no operator serviceable or replaceable parts inside the power supply ies or in the PAL System If a power supply Is not functioning contact a CTC Analytics representative The power supply for the PAL Instrument has the symbols 1 0 on the label for the power switch to switch ON OFF Any additional power supply for other devices like Cooled Stack or a Valve Module shows the symbols as shown below on the label for the power switch Power ON Power OFF The symbols shall warn the user that in a emergency case more than one power supply has to be turned
38. ot be changed To use a different Cycle or Syringe a new Method must be created 5 Assign Parameter values according to the application requirements Consult Appendix D HTC PAL LC Inj Cycle Parameter for details of the specific items HTC PAL User Manual 2 2 Edit View Methods Method parameters excluding Cycle and Syringe can be viewed and changed from the Method menu as follows Menu Methods Method name Enter NI Scroll to and select the Parameter to be changed Assign the new value and press the ENTER key 3 kitfrom Parameter List by pressing either the Home function key F4 to return to the top level Job Queue menu or the ESCape key to return to the previous menu 4 Method contents may be viewed from the Job Queue displays by selecting the desired Job pressing ENTER and the View Method function key 2 3 Delete Methods Methods can be deleted from the Method menu Methods in use by an active Job cannot be deleted Complete the following menu selections to delete a Method Menu Methods method name Del Method 3 HTC PAL User Manual Job and Job Queue 3 1 Building and Starting a Job Queue Power up the HTC PAL The JOB QUEUE screen is displayed JOB QUEUE 05 Stk1 01 001 096 eml 1 001 013 Method As CT Menu Add Del Start Job Job Figure 3 Example Job Queue Screen Load a sample Tray onto an available location in a Tray Holder or Stack Note the corresponding Tray nam
39. played Before proceeding with the remaining steps switch OFF power to the HTC PAL 21 HTC PAL User Manual Installing the LC Injection Valve Locate the blue valve drive It will have one clamp that is identical to other object clamps Loosen the clamp as above see Figure 11 Figure 11 Installing the Injection Valve Drive 2 Attach the valve drive to the X Crossrail and tighten the mounting screw note To avoid dead volume between sample injection point and detection the injection valve should be located near the detection device 5 Connect the control cable from the valve drive to AUX 4 The injection valve and rotor is pre installed on the valve drive 32 HTC PAL User Manual Installing the Fast Wash Station Attach the Wash Station assembly to the Solvent bottle holder Figure 12 Installing the Fast Wash Station Loosen the screw on the Fast Wash Station mounting clamp Attach the Fast Wash Station to the X Crossrail as close as possible to the Injection Valve This will minimize the time required to move between the two positions Tighten the mounting screw Figure 13 Fast Wash Station Assembly 99 note l HTC PAL User Manual Locate the white Teflon fittings and two lengths of Teflon tubing that connect the Wash Station valves to the wash solvent reservoirs Place the reservoirs onto their holders and attach the tubing and fittings
40. pply for Stack Cooler DW MT or Tray Cooler Peltier Element 25 HTC PAL User Manual Physical Specifications Parameter HTC PAL Systems Da X4 Height HTC PAL Depth Weight Operating and environmental Requirements Parameter Operating Temperature Range 4 to 40 C 39 to 104 F Maximum Relative Humidity 75 non condensing Bench Space At least 16 cm 6 in at the rear space for fluid waste container below the instrument and solvent containers next to the instrument Access to power switch es and power cord s Clean level and smooth surface Solid bench plate Static electricity 26 note l 6 6 1 6 2 HTC PAL User Manual Installation Unpacking the Components A HTC PAL system Is shipped in two boxes One box contains the X Y Crossrail assembly the injection unit the keypad terminal standalone supports connecting cables power supply syringe kit the injection valve fast wash station assembly safety guard and miscellaneous parts The other box will typically contain Microplate Stacks and or Trayholders Open the large box containing the X Y Crossrail First remove the Injection Unit and the accessory boxes before attempting to remove the X Y Crossrail assembly Remove any loose foam packing around the X Y Crossrail Carefully lift the X Y Crossrail and remove it from the box Hold the Y Crossrail in place as the assembly is removed from the box Set the X Y Crossrail assembly on a
41. rform the maintenance procedure more frequently Clean the outside of the instrument Use only a soft lint free paper or cloth dampened with mild soap and water Clean Instrument syringe and surfaces Weekly or as needed Every 1500 to 2000 penetrations Check the tightness of the needle in the needle seal Replace the valve rotor Annually or more a year depending on the throughput and quality of the sample solution and Mobile Phase Syringe Plunger has to be replaced on a regular base The interval is highly dependent on the application throughput quality of sample solution particles etc washing solvent It is advisable to check the tightness of the plunger on a regular base to gain the experience with the own application Weekly or as needed Replace the valve needle seal Replace the valve rotor Replace the syringe plunger The other steps are described in the Preventative Maintenance Procedure Specific kits for the GC or the HPLC techniques are available The kits include the parts which shall be changed annually PM Kit1 for HPLC and PM Kit2 for GC technique note There are no operator serviceable or replaceable parts inside the power supplv ies or the PAL System In case of any failure contact a representative of CTC Analytics 54 Job Queue Job Cycle Method Method Parameters Object Trayholder Stack Tray Tray Type HTC PAL User Manual Appendices Definiti
42. t 1 to an existing signal Object Injected complete the following menu selections Out Signals Injected Destination SW Outl PAL s are shipped with all Cycle events pre defined as shown in the table below If a different physical signal needs to be used then it will be necessary to assign a new Event to the signal Object If a signal is to be ignored then it s corresponding Event must be set to Immediat Standard Assignment for HTC PAL Description Default INTERFACE Injection Cycles Events Pin Start Svnc Signal Start a cycle Input from LC or TIL InI 7 data system 8 GND Inject Sync Signal Inject Immediat READY to INJECT Ignore Injected Out Signal Is activated at the moment when SW Outl 3 the sample has been injected 4 Running Out Signal Active as long as the Job Queue is Off being processed and the PAL is not in an error state 46 note l 11 11 1 HTC PAL User Manual PAL Loader Program The following procedures require a PC with Windows 95 98 NT 2000 XP Operating System the supplied PAL Loader program Version 1 1 or higher the supplied 9 pin brown cable Part RS9B 3000 and the correct version of the PAL firmware to be loaded Backing Up the PAL Control Program The following procedure should be performed after the installation of a new PAL System is completed Furthermore periodically System backups ensures that an updated configuration of the PAL is available any time
43. t s location A Stack Is a particular type of Trayholder that is designed to hold micro plates A six drawer Stack holds 12 standard micro plates two in each drawer A three drawer Stack holds six deep well micro plates two in each drawer A Tray that holds multiple samples Trays are defined by designating the Tray Type see below and the Trayholder Tray names are used to identify the sample source within a PAL Job A Tray Type defines the pattern and sampling sequence of sample locations within a Tray Standard Tray Types include shallow and deep well 96 position Microplates and Trays that hold 1 2 10 and 20ml vials 55 HTC PAL User Manual Conventions of Naming This section recommends standard naming conventions for HTC PAL Trays Tray Types and Tray holders Following these conventions will allow HTC PAL s to be pre configured for certain applications will simplify software backups and application development and will improve technical support and training Tray Type M196 DW96 M1384 VI200 VT98 VI78 VI54 Tray Description Standard 96 position microplates Deep well 96 position microplates High density 384 position microplates Vial Tray 200 positions 10 X 20 for 7mm micro vials 1 0 ml Vial Tray 98 positions 7 X 14 for 12mm vials 2 0 ml Vial Tray 78 positions 6 X 13 for 7mm micro vials 1 0ml opposite side of 98 positions Tray Vial Tray 54 positions 6 X 9 for 12mm vials 2
44. te l 2 6 3 HTC PAL User Manual Install the Trayholder with the mounting clamp teeth fitting into the grooves on the bottom of the X Crossrail The Trayholder should be near the center of the X Crossrail Be sure that the clamps fit completely into the grooves Alternately tighten the two Torx screws until the two mounting clamps are firmly in place Double check if the two Trayholder clamps are correctly attached to the X Crossrail see Figure 5 page 19 Electrical connections Before defining the HTC PAL object positions make sure the LC Injection Valve Fast Wash Station and Keypad Terminal are correctly connected to the HTC PAL X axis rear side see Figure 17 Figure 17 HTC PAL Electrical Connections Always switch OFF the PAL power supply before connecting or disconnecting any HTC PAL accessories cable Keypad Terminal gt SER3 Terminal LC Injection Valve AUX Fast Wash Station gt WASHSTATION 56 note l HTC PAL User Manual 7 HTCPAL Object Positions 7 1 Defining Object Reference Positions Remove the Syringe Adapter from the Injection Unit before performing the following steps The objective is to define the Reference Positions for standard HTC PAL Objects Make sure the Trayholder s Valve s and Fast Wash Station are properly mounted to the PAL X axis The following description is an example how to teach the Reference Position for a Trayholder The described procedure is common to all
45. ted Set Pos is used to define the Chang Syringe position The Injection Unit moves to its HOME position and the Job Queue Menu is displayed The following Syringe Parameters may be changed by selecting the particular item Item Actual Id Fill Volume Fill Strokes Pullup Del Fill Speed Eject Speed Inject Speed Plunger Pos Description Indicates the Identification number ID of the currently inserted syringe If the syringe detection system Is set to manual the extension man is displayed This value can not be changed This parameter serves to control the filling of the syringe It may happen that air bubbles remain below the plunger after the first pull up If the plunger is moved up and down several times see Fill Strokes these air bubbles are worked out With this operation the syringe may be completely filled even when using very small sample volumes Number of Fill Strokes All Fill Strokes except the last one use the selected Fill Volume If the selected Sample volume is higher than the Fill Volume the Sample Volume Is used for all Fill Strokes If zero is selected the plunger is pulled up only once using the Sample volume value Using this item a delay time between sample pullup and ejection while filling the syringe can be selected The same delay time is used after the last plunger pullup until the syringe needle is moved out of the sample vial well It is only used if more than one Fill Stroke is s
46. tem in a list Function Key Definitions Function Keys Press STOP to abort operation Press the inner knob to select an item highlighted by the cursor bars Press again to ENTER the selection Rotate the outer knob to move the cursor bars Press ESCape to return to the previous menu Menu Methods 1 1 HTC PAL User Manual Menu Screens Different menu screens are displayed depending on the HTC PAL operating state and the particular function being accessed by the operator All menu screens have the same basic format The menu title is displayed at the top of the screen A list of items is displayed below the title The date and time or a status is shown in the highlighted area above the Function key labels on the bottom of the screen Method A CYCLE LC Inj SYRINGE 100w Sample Volume zenem Figure 2 Accessing a Method Screen Function Keys Options for a particular menu are assigned to the corresponding function keys F1 F2 F3 and F4 directly below each function key label Pressing the function key defined as Home will always return to the Job Queue menu ESCape and STOP Keys Press the ESCape key to return to the previous menu Press the STOP key to abort the current Cycle Job or Job Queue Scroll Knob and ENTER Button Rotate the outer knob to scroll through items in a menu list To select a highlighted item press the central knob ENTER button Then use the outer knob to scroll through available opt
47. the syringe needle A stainless steel ferrule is tightened around the Teflon sleeve to ensure a leak proof fit To ensure reproducible sample injection and minimize carryover it is critical that The valve needle guide and the valve needle seal are installed properly P N PAL NdlSeal pkg of 10 The valve needle seal is changed every 1500 2000 penetrations The needle penetration depth is set accurately see chapter 9 3 Injection Valve Needle Penetration 44 note l note l HTC PAL User Manual 9 3 Injection Valve Needle Penetration Before performing the following steps make sure the Object Position X Y Z for LC Vivi is properly defined and a syringe adapter including a syringe is inserted in the Injection Unit see chapter 7 2 and 8 3 Complete the following steps to define the Valve Needle Penetration Depth In the Object Class Injectors choose the Object LC Vlv1 The following screen is displayed Menu Setup Injector LC Vivl Position Y 2 6mm Position Z 86 0mm Needle Penetr 20 0mm Valve DC6 1 C Objects 1998 08 31 11 20 25 Check Clear Movto Home Pos Pos Zero Figure 28 Menu Screen Valve Needle Penetration Depth Press the F1 button Check Pos The Injection Unit moves to the previous defined LC Vlvi position Highlight the ttem Needle Penetr with the cursor bars and press ENTER Slowly rotate the outer knob to adjust the Needle Penetration Depth The syringe moves
48. uog5og x u gsag UpIHL Ie 910593 led S592IY 7 uonsog A U0N60d x u nsog DES ied 31052 je 5929 7 WONSOd A UOQEOJ y UDIBOg ppeis 61 E External Connectors Connector SERI Pin Signal Name DCD RXD TXD DTR GND DSR RTS CTS RI WO CON DMD OT PI WIN Connector SER2 SER3 Pin Signal Name GND 5V reserved reserved O YA UB WN O 014 co HTC PAL User Manual 62 HTC PAL User Manual Connector INTERFACE 8 1 99999999 f o 3999999 15 9 Signal Name Pwr Outl Pwr Out2 SW Outi NO SW Outl COM SW Out2 NO Opto In1 TIL Inl GND Pwr Outl Pwr Out2 TTL In2 SW Out2 COM TTL In3 Opto InT 5V O 2 al a a KO Mm AINNI O 63 HTC PAL User Manual Connector AUX Pin Signal Name 1 3 Motor A1 2 4 Motor A2 5 7 Motor B1 6 8 Motor B2 10 16 18 GND 11 reserved 12 reserved 13 Sens 14 45V 15 17 reserved 19 20 436V Connector WASHSTATION l 2 l 2 Pin Signal Name I Pwr Out1 l Pwr Outl 24 Pwr Out2 2 Pwr Out2 64 HTC PAL User Manual This page is intentionally blank 65
49. vided into three major sections HTC PAL Operating Instructions Section A HTC PAL Description and Installation Section B Appendices The HTC PAL Operating instructions in Section A are intended for infrequent PAL users or new users that are experienced in using automated systems to perform existing analytical methods The HTC PAL must be installed and set up properly before the Operating Instructions in Section A can be used Users who are installing a HTC PAL system HTC PAL accessories or who need to make adjustments to an installed system should consult HTC PAL Description and Installation In Section B The Appendices provide useful information such as the Software Flow Chart Definition of Terms or the HTC PAL Accessories guide Job HTC PAL User Manual A HTCPAL Operating Instructions 1 Using the Control Terminal The following procedures present the kev steps required to set up and process multiple groups of samples with the HTC PAL It is intended to provide an overview for new users and a reminder for infrequent users The HTC PAL and all accessories should be installed and Objects defined correctly A Syringe of the type called for in any Method to be used should also be installed Figure 1 illustrates the PAL Control Terminal and the conventions used to enter edit and view information JOB QUEUE ACTIVE Figure 1 PAL Control Terminal and Conventions Menu Title Cursor bars highlight an i
50. xternal or internal grounding connections Tampering with or disconnecting these connections could endanger you and or damage the PAL System The instrument is properly grounded in accordance with these regulations when shipped You do not need to make any changes to the electrical connections or the instrument s chassis to ensure safe operation The combination of a PAL System with a LC MS System does require the safety measure as described by the LC MS System manufacturer Detailed instructions for the safety grounding on the LC MS system are outlined in the corresponding operating installation manual CTC Analytics recommends to use a grounding cable connected on one side at the Injection Valve Loop or any other suitable direct metallic contact and the other side at an appropriate grounding point at the LC MS System This supplementary grounding measure will support the safety strategy of the LC MS System manufacturer HTC PAL User Manual A Do not turn the instrument on if you suspect that it has incurred any kind of electrical damage Instead disconnect the power cord and contact a CTC Analytics representative for a product evaluation Do not attempt to use the instrument until it has been evaluated Electrical damage may have occurred if the PAL System shows visible signs of damage exposure to any liquids or has been transported under severe stress Damage can also result if the instrument is stored for prolonged periods under unfav
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