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CellaVision® ProficiencySoftware - CellaVision Proficiency Software

1. Schistocytoss Helmet cels Sickle cels Spherocytosis Elliptocytosis Ovalocytosis Tear drop cells Stomatocytosis Acanthocytosis Echinocytosis Howell Joly Pappenheimer Basophilic stippling Parasites O CH Malaria oe i RBC characterization Reportallas ormal 1 RBC tab 2 Zoom slider 3 Magnified area 4 Report all as normal General Workflow 1 Click on the RBC 1 tab 2 Zoom in to the RBC image using either the mouse wheel or the zoom slider 2 The RBC image corresponds to the area of 8 microscopic high power fields HPF 100x objective and a 22mm ocular Note Be aware that RBC images captured using a Cella Vision Image Capture System can be of different magnifications 3 Move around the image by clicking and dragging it 4 Double click anywhere on the RBC image to magnify that area Move the mouse around the RBC image to move the position of the magnified area 3 Click again to close it 5 Grade the RBC morphology using the radio buttons in the side panel If required reset all morphologies grades to a normal level by clicking Report all as normal 4 User s Manual PM 10509 2013 03 04 41 CELLAVISION CellaVision Proficiency PARTICIPANT EE RBC NT Grades Normal Normal level e aaa
2. 3 Select individual orders by clicking on the check mark next to the orders Select multiple orders at once by holding down SHIFT or CTRL whilst clicking on order rows 4 Click Mark all highlighted Select and add all orders m the database by clicking the check box in the column header Note Orders that have already been marked for transfer in a CellaVision DM Software are indicated by the ra Mark for Transfer Tool icon The option to mark orders for transfer is only available in CellaVision DM Software version 4 0 or higher Right click in the order list view to mark an order for transfer 5 Click Transfer in the Transfer selection panel to the right 6 Select a location on the hard drive and save the files Step 3 Upload 1 In the CellaVision Proficiency Software navigate to the SLIDES tab and click Upload slides 2 Navigate to the previously saved slide files and select one or more slides for upload 3 Click Open The slides may take a few minutes to upload Note No patient identification information is transferred with the slides All transferred cell images are copies from the original 20 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Software EN MER 2 3 2 The SLIDES Tab 2 Tam SLIDES n PARTICIPANTS SETTINGS A Kapferer Log ou 9 CELLAVISION OF TESTS Tam Slides with peripheral blood 12 14 Z Slide ID Analyze
3. If a test closes before all Participants have verified all slides it will reduce the data available for the results statistics Any slide a Participant has not completed will be excluded from the results for that particular Participant End dates of ongoing tests can be extended as described in 2 2 3 3 Extending the Test End Date Do not set an automatic If no end date 1s set the test will be flagged as completion date completed as soon as all Participants have verified all slides 8 PM 10509 2013 03 04 User s Manual CellaVision Proficiency CELLAVISION EXAMINER User s Manual Visible Information Show patient information Make reference cells available Show additional slide data Give early access to results Test Comment Write a test comment Software If checked patient information associated with a slide if available will be visible to all Participants See 2 3 3 5 Add or Edit Patient Information for more information If checked Participants are able to open reference cell libraries whilst they are verifying slides If no reference cells are available this setting is disabled See 2 3 4 4 White Blood Cell Classification Create WBC Reference Libraries for more information If checked additional data associated with a slide if available will be visible to all Participants See 2 3 3 6 Add or Delete Additional Slide Data for more information If checked individual Partic
4. 4 Infographics 44 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency PARTICIPANT Software Click the Summary of results tab to see an overview of how Participants performed in the test Participant names are anonymized but your results are indicated by You 3 3 4 1 Bar Chart The bar chart 1 shows the average WBC and RBC agreement for each Participant displayed as percentage values The higher the agreement the better a Participant is considered to have performed in the test Switch between using either the Examiner or Majority result as a reference for the agreement using the radio buttons in the Compare to 2 box The Agreement percentages 3 box shows the values used for drawing the bars in the bar chart Sort the bar chart using the drop down menu Filter the bar chart using the drop down menus Only available in the Enterprise version 3 3 4 2 Infographics The infographics 4 display the following key metrics of the test e Total Participant number e Average WBC and RBC agreement for all Participants compared to the reference selected in the compare to box Examiner or Majority result e Highest and lowest WBC and RBC agreements compared to the reference selected in the Compare to box Examiner or Majority result 3 3 5 WBC Classes Click the WBC classes tab to see the results of WBC classification on a class level Switch between Examiner and Majority in the comp
5. 20 TROUBLE SHOOTING ereen 33 2 6 1 No Slides Shown in Wizard Gren 33 2 6 2 Missing Slides in Wizard Step 1 33 2 6 3 No Participants Shown in Wizard Step 2 33 IV PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Software 2 6 4 No Results Available in Test Results View 33 S PARIICIPANT Fri 34 EENet 34 EOIN 34 3 1 2 Retrieving the Hasesword NEEN 34 TN NES 35 o De TE To T D e ie 35 eZ L VIG WANG T TOS ae E 36 SLO Wel e Elte 37 TSN ho e 42 oo VIEWING EST RE RE 42 STENE 42 3 3 2 Opening a Completed lest 43 3 3 3 The EC 43 JA TeS SUMMA E 44 Ee 45 DO EEE 47 SE E ett Le 50 E ene Cl EE 50 APPENDIX A BUTTONS AND SYMBOLS eevvrrernnnnnnnnnnnnnnnnnnnnnnnnnnnnnnsnnnnnnnnnnensnnnnnnnnnnnnr 51 EE EEE EEE ai 51 APS 51 1 19 0 EEE EE 52 APPENDIX B RECOMMENDED WORKFLOW rnnnnovnnnennnnnnnnnnnnnnnnsnnnnnnnnnnennnnnnnnnnnnner 53 APPENDIX C CALGULATIONS annnnnnneennnnnnnnnnnnensnnnnnnnnnnnnensnnnnnnnnnnnnensnnnnnnnnnnenennnnnnnnnenne 55 APPENDIA DGLOSSARY ili 57 User s Manual PM 10509 2013 03 04 V CELLAVISION CellaVision Proficiency INTRODUCTION 1 2 1 3 Software INTRODUCTION General Description and Intended Use The CellaVision Proficiency Software is a tool for education and proficiency testing of manual blood cell differentials in laboratories There are two types of users in the CellaVision Proficiency S
6. 3 Click Log in If more than one account are associated with the login credentials a drop down menu will be shown after logging in where the desired account can be chosen Retrieving the Password 1 In the login screen click the Forgot password link 2 Enter your email address and click Reset password 3 A new password will be sent to this email address Customizing the Workspace The first log in to a CellaVision Proficiency Software account prompts the Customize your account window to open Take a minute to e Choose a new password to replace the temporary password e Set the preferred Language for the user interface e Check that the Email address is correct Account settings can be changed at any time later in the SETTINGS tab See 2 5 SETTINGS for more information PM 10509 2013 03 04 3 CELLAVISION EXAMINER CellaVision Proficiency Software 2 2 CREATING MANAGING AND EVALUATING TESTS 2 2 1 The TESTS Tab 1 CELLAVISION Tame SLIDES A PARTICIPANTS SETTINGS A A Kapferer Logout 9 2 K Create a new test Create 5 A Under construction Test 3 Hodgkins Lymphoma Test 3 Hodgkins Lymphoma Ends on 2012 12 12 Double click to continue setup i 3 EG Test comment Ongoing 4 BX includes participants CH Completed Sie Ges 1 Create a new test 2 Under construction 3 Ongoing 4 Completed 5 Detailed view The TESTS tab is the starting point for creating a new test 1
7. Pappenheimer Basophilic stippling Parasites ORM amp 2 N La foe di OH w Ze OH O DI D Reportallas normal 1 RBC tab 2 Zoom slider 3 Magnified area 26 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Software E E General Workflow Click on the RBC 1 tab 2 Zoom in to the RBC image using either the mouse wheel or the zoom slider 2 The RBC image corresponds to the area of 8 microscopic high power fields HPF 100x objective and a 22mm ocular Note Be aware that RBC images captured using a CellaVision Image Capture System can be of different magnifications LA Move around the image by clicking and dragging it D Double click anywhere on the RBC image to magnify that area Move the mouse around the RBC image to move the position of the magnified area 3 Click again to close it 5 Grade the RBC morphology using the radio buttons in the side panel If required reset all morphologies grades to a normal level by clicking Report all as normal RBC Characterization Grades Normal level Morphology is present at a low level Morphology is present at a moderate level Morphology is present at a high level Using the Ruler In the top left hand corner of the RBC image there is a cross shaped ruler that represents 14 um across Click on the ruler and drag it to
8. tab in the side panel to see how the WBC classification results compare between you and a chosen reference Change the reference using the drop down menu in the Compare 3 box The first two columns in the Disagreement results 4 table show the number of cells you and the reference classified to each class The third column shows the number of cells where the classification differs Sort WBC classes with the most disagreements to the top of the list by clicking on the table headers in the Disagreement results 4 table Click on a cell class name to show cell images with disagreements in the main view 2 The two differing classifications are shown above the cell image Use the arrow buttons underneath the cell image to click through all disagreements of that class Cell comments if available are shown underneath the cell image 3 3 6 3 Showing Cell Libraries Click Open cell class to show all cells for that slide that were classified to this class by the Examiner Use the drop down menu to change to a different class User s Manual PM 10509 2013 03 04 49 CELLAVISION CellaVision Proficiency Software PARTICIPANT 3 3 7 RBC Characteristics Cc E L L AV S I Oo N de TESTS Results view Browse test results a J Stevens Log gu Is Results for re Close Summary WBC WBC RBC a of results classes cells Show data for slide Filter AI Slide 1 RBC 1 slide Show RBC image E 1 degree
9. CELLAVISION CellaVision Proficiency Software EE Customize the Layout Switch between a one class two class or all classes layout using the Layout Toggle buttons Increase or decrease the size of the cell images using the Zoom Slider Magnify Individual WBC 1 Double click on a cell 2 Use the mouse wheel to change the zoom level 3 Use the arrow keys on the keyboard to show the next cell in the gallery 4 Click Close to close the magnified view Add or Edit Individual Cell Comments 1 Double click on a cell 2 Click in the comment box underneath the magnified cell and write a comment 3 Click Save to save the comment 4 Click Close to close the magnified view Cells with comments are marked by a comment icon in the top right corner Cell Marker The cell marker is a square marking the cell for identification Click to show hide the square Split WBC 1 Right click on a cell and choose Split cell from the menu 2 Click on the center of all individual cells to mark them 3 Click OK to split the cell The new cells are added to the same class as the original cell Delete WBC Delete cells by dragging them to the Trash class in the side panel or by choosing Trash from the right click menu Deleted cells are not visible for Participants User s Manual PM 10509 2013 03 04 25 CELLAVISION CellaVision Proficiency SINE Software Retrieve deleted cells by opening the Trash clas
10. Close to close the magnified view Add or Edit Individual Cell Comments 1 Double click on a cell A magnified version of that cell will open User s Manual PM 10509 2013 03 04 39 CELLAVISION CellaVision Proficiency PARTICIPANT Software 2 Click in the Comment box underneath the magnified cell and write a comment 3 Click Save to save the comment 4 Click Close to close the magnified view Cells with comments are marked by a comment icon in the top right corner i p u Ee T We b de EF fa pe i K 7 Li E Cell Marker The cell marker is a square that surrounds the cell for identification Click to show hide the square WBC Reference Libraries 1 Switch to either the one class or two class layout 2 Open the cell class of interest 3 Click Reference cells The reference cell gallery for that class will open as a separate gallery Note Reference libraries are only available if specified so by the Examiner 40 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency PARTICIPANT Software 3 2 3 5 Red Blood Cell Characterization CELLAVISION R TESTS Verification of Slide 1 Ai Stevens Logout 9 Slide 1 Q Sign slide Closes 0123 Polychromasia Hypochromasia Anisocytosis Microcytosis Macrocytosis Poikilocytosis Target cels
11. It also displays a list of all tests that are currently either under Construction 2 Ongoing 3 or Completed 4 sorted by test creation date Test results can be accessed from this tab by double clicking on a completed test See also 2 2 4 Viewing Test Results for more information Click on any test in the list to view more detailed information in the detailed view 5 2 2 1 1 Under Construction Tests A test stays under construction until the creation of the test is finished and the test is distributed to the Participants Double click on an under construction test to continue working on it Under construction tests are not visible to the Participants 2 2 1 2 Ongoing Tests Once test creation is finished the test 1s flagged as ongoing and becomes visible to its Participants PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Software E 2 2 1 3 2 2 2 CELLAVISION Basic test Participants can now classify and or characterize cells Double click on an ongoing test to view the Participants progress see also 2 2 3 Monitoring Ongoing Tests Completed Tests A test is flagged as completed when one of the following applies e The completion date of the test has been reached e All Participants have completed and signed all slides in the test e The test is manually set to completed by the Examiner see also 2 2 3 2 Stop an Ongoing Test An email is sent to both the Examiner and the
12. Participants when a test is completed Double click on a completed test to view the results see also 2 2 4 Viewing Test Results Creating a New Test 1 Enter a test name and click Create 2 Select one or more slides in the Available slides list s Click on the column headers to sort the list If no slides are shown click on the SLIDES tab and follow instructions see also 2 6 1 No Slides Shown in Wizard Step 1 and 2 6 2 Missing Slides in Wizard Step 1 _ A PARTICIPANTS SETTINGS AT User Logout 9 a la H pes Step 1 Step 2 Step 3 Step 4 Select slides Select the participants Select preferences Review and finish Available slides Selected slides Peripheral blood samples Slide ID Analyzed Patientinformation Slide note WBC RBC 1003M 2005 04 05 Lymphocytes Variant v v 1004M 2005 04 05 Normal v v 1005M 2005 04 05 Normal v v 1006M 2005 04 05 Immature Gran v v Body fluid samples Slide ID Analyzed Patent information Slide note 1008 2012 06 12 CSF Exit Next User s Manual PM 10509 2013 03 04 5 CELLAVISION SE SS EXAMINER CellaVision Proficiency Software Note A maximum of 20 slides can be added per test Tests can only include slides with either peripheral blood or body fluid samples but not both 3 Click to add the selected slides to the test and then click Next to proceed to Step 2 of the wizard 4 Select one or more Participants in the Avai
13. cells where the Participant and reference agreed on the classification The orange part indicates where they disagreed 14 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Software EE Change Participant using the drop down menu in the Display box One Cell Class All Participants CELLAVISION Tame SLIDES PARTICIPANTS SETTINGS AA Kapferer Logout 9 In Results for re i Close Summary WBC WBC RBC EEE OT FESUNS x ell haracteristic Show data for slides Slide 1 1004M WBC classes Total numbers 1 slide Sorted by Slide 2 1005M Lymphocyte C Examiner result DS Number of cells where classification agrees with examiner results DI Number of cells where classification disagrees with examiner results Compare to Anna 4 Examiner Majority Display One participant all cell classes Pau v One cell class all participants Lymphocyte When selecting One cell class all participants the total number of cells classified to the selected cell class by all Participants as well as the reference is shown The top bar dark grey shows the reference result The following bars show the Participant results The blue part of the bar indicates the number of cells where the Participant and reference agreed on the classification The orange part indicates where they disagreed Change cell class using the drop down menu in the dis
14. generated in the detailed view The file format must be either a PDF file Microsoft Word document or TXT JPG PNG GIF or BMP file The maximum allowed file size is 3MB and only two files can be added per slide 22 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Software EE 4 Click on a link to open it The file will open in an program suitable for that file type 5 To delete additional slide data click on W Delete next to the link Note Additional slide data is only visible to the Participants if the setting Show additional slide data is selected in the test creation wizard 2 3 4 Verifying Slides 2 3 4 1 Opening a Slide for Verification In the SLIDES tab double click on a slide to verify it 2 3 4 2 The Verification View 1 CELLAVISION EE en PARTICIPANTS SETTINGS A Kapferer Logout 9 1214Z Makasdone Close Eosinophil Basophil Lymphocyte 96 5 111 Unidentified Band neutrophil SEI Segmented neutrophil D Reference cells CFA Lymphocyte D Monocyte Promyelocyte Myelocyte 2 Metamyelocyte Promonocyte E om Prolymphocyte Blast Lymphocyte variant form Plasma cell Erythroblast Giant thrombocyte Thrombocyte aggregation Megakaryocyte Smudge cell 1 WBC RBC navigation 2 Collapse Expand 3 Main view WBC RBC Navigation Switch between WBC and RBC 1 verification using th
15. list is shown in brackets next to the heading All cells above the cell image The Cell classifications box 3 shows the cell classes and number of total classifications to each cell class for the currently shown cell It also shows which slide the cell originates from The Individual cell classifications 4 box shows how the Examiner and each Participant classified the cell Use the arrow buttons underneath the cell to click through all cells The cells are sorted so that the cells with highest number of different classifications are at the beginning of the list 48 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Sowara PARTICIPANT 3 3 6 2 Compare Individuals a CELLAVISION de TESTS results view Browse test results J Stevens Logout Ila Results for be Giona Summary i WBC RBC of results 0 classes characteristics Show data for slides WBC cells Compare individuals 1 slide Slide 1 Slide 2 Segmented neutrophil Disagreements 2 Examiner Segmented neutrophil _ You Band neutrophil 2 FA a o D Comparison results Examiner You Segmente 58 58 Monocyte 10 10 Band 7 7 Lymphocyte 28 28 Open cell class Smudge cell 1 1 Giant 1 1 Lymphocyt 2 2 o e e e e Me WM Eosinophil 1 1 TOTAL 108 108 2 Compare individuals 2 Main view 3 Compare 4 Disagreement results Click on the Compare individuals 1
16. you The blue part of the bar indicates the number of cells where you and reference agreed on the classification The orange part indicates where you disagreed 46 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency PARTICIPANT Software 3 3 5 2 One cell class all participants CELLAVISION de TESTS Results view Browse test results J Stevens Logout In Results for rbc 2 Close Summary d WBC WBC l RBC of resul i ha pristi Show data for slides Filter Ia 3 Slide 1 WBC classes Total numbers 1 slide Sorted by e Slide 2 Segmented neutrophil Examiner result ia Number of cells where classification agrees with examiner results o Number of cells where classification disagrees with examiner results User 1 User 2 Compare to User 3 4 O Examiner Majority User 4 Display ll participant all cell classes User 5 One cell class all participants pest td nd dio cia User 6 Segmented neutrophil la 3 c User 8 User 9 A CO Q ep n da 72 When selecting One cell class all participants the total number of cells classified to the selected cell class by all Participants as well as the reference is shown The top bar dark grey shows the reference result The following bars show the Participant results The blue part of the bar indicates the number of cells where the Participant and reference a
17. 3 04 33 CELLAVISION PARTICIPANT CellaVision Proficiency 3 3 1 3 1 1 Software PARTICIPANT The following chapters are intended for the Participant An Examiner cannot access these parts of the program GETTING STARTED Logging in 1 Open an internet browser window and go to https www cellavision proficiency software com Log in CellaVision Proficiency Software Email or username Password Forgot password Log in New user Create a free trial account CELLAVISION 2 Enter your email username and password 3 Click Log in Retrieving the Password 1 In the login screen click the Forgot password link 2 Enter your email address and click Reset password 3 A new password will be sent to this email address Note Ifno email address is associated with your account please contact your Examiner 34 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency E Ee PARTICIPANT 3 2 TAKINGATEST 3 2 1 The TESTS Tab CELLAVISION de TESTS welome James You have the 3 mg tests in your account 4 BJ Stevens Logout 1 amp Ongoing Ae N test number six 2012 11 21 2012 11 30 Double click to open test 9 6 e amp Completed 2 Harry test 2012 11 21 open end 1 Ongoing 2 Completed 3 Test completion dates 4 Test comments 5 Test results 6 Account settings The TESTS tab lists all Ongoin
18. AMINER Empty field Slide is not being used in an ongoing or completed test and is not locked Slide is locked Verification cannot be changed and the slide cannot be deleted 2 3 3 2 Sorting Click on the column headers to sort the list 2 3 3 3 Edit the Slide Identifier 1 Click on a slide in the slide list to focus the detailed view to that slide 2 Click on the slide identifier in the detailed view 3 Edit the identifier and click Save 2 3 3 4 Add or Edit Slide Notes Slide notes are only visible to the Examiner and serve as an internal memo about that slide 1 Click on a slide in the slide list to focus the detailed view to that slide 2 Click on Add note or if a slide note already exists on the note itself 3 Add or edit the note 4 Click Save to save the change or Cancel to return the slide note to its previous state 2 3 3 5 Add or Edit Patient Information 1 Click on a slide in the slide list to focus the detailed view to that slide 2 Click on Add information or if patient information already exists on the information itself 3 Add or edit the information 4 Click Save to save the change or Cancel to return the patient information to its previous state 2 3 3 6 Add or Delete Additional Slide Data 1 Click on a slide in the slide list to focus the detailed view to that slide 2 Click Upload file in the detailed view 3 Navigate to the files on the hard drive and click Open A link to the file is
19. CellaVision Proficiency Soft CELLAVISION de TESTS Results View Browse the results of your tests amp m Pongthaisong Logout Results for Test Case Number One 8 x K Less ga Show data for slides 1003W WBC View classifications 3 sides 1004W i All cells 242 1006W 1007W 1008W Cell classifications Slide 1003W Cell 89 Total Classifications Lymphocyte Segmented Neutrophil Eosinophil Individual cell classifications Slide 1003W Cell 89 Cell class Examiner Lymphocyte You Segmented neutrophil User 1 Lymphocyte User 2 Eosinophil User 3 Lymphocyte lt 1 of 242 User 4 Lymphocyte P Examiner Round or kidney shaped nucleus diffusely lumpy to compact USER S MANUAL 2 0 CELLAVISION CellaVision Proficiency Software User s Manual CELLAVISION CellaVision Proficiency Software Preface CellaVision is a trademark of Cella Vision AB All other trademarks used in this document are property of their respective owners No part of this document or the products it describes may be reproduced or transmitted by any means or in any form without prior consent in writing from CellaVision AB Article No PM 10509 Revision 2013 03 04 Copyright 2013 CellaVision AB All rights reserved Headquarter CellaVision AB Ideon Science Park SE 223 70 Lund Sweden Phone 46 0 46 286 44 00 Fax 46 0 46 286 44 70 User s Manual PM 10509 2013 03 04 iii CELLA
20. CellaVision Proficiency Calculations ORNA Example The table below shows how the RBC agreement is calculated for one slide Reference Participant Morphology Characterization Characterization ODSmax indaitt Polychromasia Hypochromasia Anisocytosis Microcytosis Marcocytosis Poikilocytosis Target cells Schistocytosis Helmet cells Sickle cells Spherocytosis Elliptocytosis Ovalocytosis Tear drop cells Stomatocytosis Acanthocytosis Echinocytosis Howell Jolly Pappenheimer Basophilic stippling Parasites d iffmax indiota 46 20 ind 1 total 100 1 22 x 100 56 5 The RBC skillfulness is iff max 46 56 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Glossary Software Appendix D Glossary The person assessing proficiency Participant The person taking the proficiency tests Lab Supervisor A Participant given allowance by the Examiner to view the test results with Participant names within a specific category performed by the Examiner WBC Agreement WBC Agreement shows how well the Participant s classification agrees percentage with the reference RBC Agreement A measure that describes how close to the reference result a Participant has characterized the RBC The process of assigning WBC s to pre defined classes The process of assigning RBCs to pre defined classes Verification The process of verifying existing cell classificati
21. IPANT Software If the majority cannot be decided unanimously one of the cell classes characterizations is at random used as majority 3 3 1 3 Reference The reference is either the Examiner result or Majority result depending on the selection made by the viewer 3 3 1 4 WBC Agreement WBC Agreement shows how well the Participant s classification agrees percentage with the reference 3 3 1 5 RBC Agreement The RBC Agreement shows how well the Participant s characterization agrees percentage with the reference 3 3 2 Openinga Completed Test In the TESTS tab double click on a completed test to open the results view 3 3 3 The Results View 1 Vw Results for Test 1 Basic Peripheral Blood e Summa WBC WBC i classes cells characteristics Show data for slides Slide 1 1004M Summary Slide 2 1005M 1 slide nin 1 Results view navigation 2 Collapse Expand 3 Slide list 4 Slide comments 5 Slide counter 3 3 3 1 Results View Navigation Click the results view navigation 1 tabs to browse the results Test results are automatically compiled to show e A summary of results e WBC classification statistics on a class level e WBC classification statistics on a cell level User s Manual PM 10509 2013 03 04 A3 CELLAVISION Ee SS PARTICIPANT CellaVision Proficiency Software e RBC characterization statistics 3 3 3 2 Collapse Expand Click on the arrow to co
22. Reference Libraries for more information 2 2 4 7 RBC Characteristics CELLAVISION Tame SLIDES A PARTICIPANTS SETTINGS AA Kapferer Logout 9 2 ja 1 i Its Results for rbe coss men Summary WBC WBC RBC of results Classes cells Show data for slide Filter 1 slide Show RBC i Slide 1 1004M RBC aa em OE nage z Slide 2 1005M E 1 degree difference to reference D 2 degree difference to reference fei 3 degree difference to reference Compare to Characterization degree Examiner Majority Examiner Majority Paul David Anna Mary Linda John Karen Suzan Laura Tear drop cells 0 Elliptocytosis Spherocytosis Sickle cells Helmet cells Schistocytosis Acanthocytosis Echinocytosis Target cells Stomatocytosis Anisocytosis Macrocytosis Poikilocytosis Parasites Howell Jolly Pappenheimer Basophilic stippling Polychromasia VO OO OO OO OO OO OO OO OO OO CH 1 O O d 0 OO 000200 OO ac E 0000 EFFE LD 0 e 00000200 00000000 CO 1 D e e oo a e e e ai a e e e 000082 amp 1 29 0 00 OO OO OO OO OO CO OO 00000005 CO 1 D e o o e E e e e Oe e e e 3202 02008208 amp 1 D OO OO OO OO OO OO ee 0202 CO oo 1 O o o 0 o CG Ge e e e e Ge e e Oe E OO 8 8 1 29 GO GO GO GO GO 000202 0202 00000 09 DN OO 000 0000 00 00 CO o co Microcytosis Hypochromasia Click the RBC characteristics tab to see the results of RBC char
23. Reset will restore the default settings 38 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency olaaa PARTICIPANT Adjust brightness and color Cancel 3 2 3 4 White Blood Cell Classification General Workflow 1 Click on the WBC tab When starting WBC classification all cells will be listed in the Unidentified class 2 Click on a WBC class in the side panel to show a gallery of all cells classified to that class in the main view When using the two class layout left click on a class to open it in the left gallery and right click on a class to open it in the right gallery Use the drop down menu in an open WBC class gallery to switch between classes 3 Classify cells by dragging and dropping them from one open class gallery to another or from an open class gallery to a class in the side panel or by right clicking on a cell and selecting a class from the menu Select multiple cells at once by pressing CTRL or SHIFT 4 Continue until all cells are classified Customize the Layout Switch between a one class two class or all classes layout using the layout toggle buttons Increase or decrease the size of the cell images using the zoom slider Magnify Individual WBC 1 Double click on a cell A magnified version of that cell will open 2 Use the mouse wheel to change the zoom level 3 Use the arrow keys on the keyboard to show the next cell in the gallery 4 Click
24. Stevens Jane Tennison 238534fa 482 Boston Clinical Lab Brookline USA 0 4 Email No email provide James Stevens 6703db8a b9c Cellavision Lab Lund Sweden 1 2 te Password Reset Sara Ulvesson 442b5487 64a Stockholms Lab Stockholm Sweden 0 3 Location Edit Ongoing tests 11 2 2012 Completed tests 23 5 2012 rbe Add participant Import CSV Ne Filter 1 Participant list 2 Detailed view Participant List The participant list 1 shows all Participants in the account It also shows the number of ongoing and completed tests for each Participant Detailed View Click on a Participant in the participant list to view the detailed view 2 28 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Software EE 2 4 2 Adding Participants 2 4 2 1 Add Participants Manually 1 Click on Add participants Add new participants Where possible add email addresses for participants to enable account notification emails First name Last name Email address Lab Region Print all _ Required fields 2 Enter First name and Last name of the Participant If available enter the Participant s Email address to enable account notification and test status emails to be sent Enter Lab Region and Country 3 Select the print checkbox if the account information of the newly created Participants should be printed 4 Click Create An email listing all newly created Participants will be sent to the Exa
25. VISION CellaVision Proficiency Software TINTRODUC HON eea E EEE cina 1 1 1 General Description and Intended Uee cccescescceeessessseeeeeeeeessssneeeeeeeeeessnes 1 12 AOL NS SEF Si IWAN TEE 1 Rig RR E E RA 1 Z EXAMINER ccr 2 2I6ETTINGSRRIELD_ inn 2 2 1 1 Creating a Free Trial Account 2 2 1 2 Requesting PN 2 2 We e e 6 e Le eree E 2 2 1 4 Retrieving the Password 3 2 1 5 Customizing the Workspace rrrrnnrrvrnnnrnnnvvennnnnnnrvrrnnnnnnrversnnnnnrvessnnnnnnnnsnnnnnr 3 2 2 CREATING MANAGING AND EVALUATING TEST 4 221 In TESTS VAD ed 4 227 Creating a New EL 5 2 2 3 Monitoring Ongoing Iesets i 10 2 2 4 Viewing Test TT IC 11 225 BOOM TET RES E 18 2 2 6 Attaching a Test Concusion ii 19 22 OC NG RE kee ee ene ie ee ee eee ee ee ee 19 2 3 VERIFYING AND MANAGING GUIDE 19 TNT ares 19 252 Me UM Ee rinite 21 225 INVA E e We Le El 21 2 34 Vening NES EE 23 2 3 5 Adding or Editing Slide Comments ii 2 2 3 6 Marking Slides as one 28 PA TA BIE Eeer Me EEE 28 2 4 ADDING AND MANAGING PARTICIPANTS mnnsrrvverrrnorrrverrnrnrrrvrrsrrnnrrvrrsnrnnnnn 28 24 1 VAG EAR EC KH Ie Tibia 28 e ell lege e Been 29 2 4 3 Filtering the Participant List EE 30 2 4 4 Managing Participants i 30 2 4 5 Setting Lab Supervisor PrivilegeS iii 30 2 4 6 Deleting Participants ek 31 ER RE 31 2 5 1 Editing Account Information 31 bl 31 2 5 3 Managing User defined Classes 32
26. Wizard Step 1 1 Navigate to the SLIDES tab 2 Upload slides to your CellaVision Proficiency Software see also 2 3 1 Uploading Slides 3 Return to the TESTS tab and double click on the respective under construction test to continue setup 2 6 2 Missing Slides in Wizard Step 1 Slides containing un classified cells cannot be included in tests and are marked with in the SLIDES tab To make these slides available for tests 1 Navigate to the SLIDES tab 2 Double click on the slide in question 3 Classify all WBC in the Unidentified class See also 3 2 3 Verifying Slides for more information 2 6 3 No Participants Shown in Wizard Step 2 1 Navigate to the PARTICIPANTS tab 2 Click Add particpants or Import CSV file and add Participants 3 Return to the TESTS tab and double click on the respective under construction test to continue setup see also 2 4 ADDING AND MANAGING PARTICIPANTS 2 6 4 No Results Available in Test Results View The following scenarios can cause no results to be shown e The test was completed early and no Participant completed the selected slide s e Nobody completed the test If possible select a different slide or a different Participant to see results If no results are available for any of the slides it 1s likely that an ongoing test was manually stopped before any slides could be completed See 2 2 3 2 Stop an Ongoing Test for more information User s Manual PM 10509 2013 0
27. acterization Switch between Examiner and Majority in the Compare to box to change the reference characterization The table shows the Examiner Majority and Participant s characterization grades for the different morphologies Cells with an orange background color indicate where the characterization differs from the reference The stronger the orange color the greater the deviation Click Show RBC image to bring up the RBC image Click outside of the image to close 1t Note RBC characterization results can only be viewed for one slide at a time 2 2 5 Exporting Test Results The Summary of results WBC classes and RBC characteristics tabs allow exporting of the presented data to a CSV file 1 In the TESTS tab double click on a completed test to open it 2 In the side panel click Export data 18 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency EXAMINER Software 3 Choose a location to save the file and click Save 2 2 6 Attaching a Test Conclusion Test Conclusions are reports created externally by the Examiner 1 Save a test conclusion as either a PDF file Microsoft Word document or JPG PNG GIF or BMP file The maximum allowed file size is 3MB Only one test conclusion can be added per test 2 In the TESTS tab click on the completed test relevant to the test conclusion to so that test is highlighted 3 In the detailed view of that test click Attach conclusion 4 Na
28. are to box to change the reference classifications Switch between showing One participant all cell classes and One cell class all participants in the Display box to show different views of the data User s Manual PM 10509 2013 03 04 A5 CELLAVISION CellaVision Proficiency PARTICIPANT ers 3 3 5 1 One participant all cell classes ier CELLAVISION de TESTS Results view Browse test results A Stevens ans In Results for rbe G Close Summary of results Show data for slides Slide 1 WBC classes Total numbers 1 slide Slide 2 You Examiner result Segmented E neutrophil E Number of cells where classification agrees with examiner results a Number of cells where classification disagrees with examiner results Lymphocyte Monocyte Compare to 4 Examiner Majority Lymphocyte variant form Display One participant all cell classes a Eosinophil You One cell class all participants Unidentified e Giant thrombocyte Band neutrophil Smudge cell o um When selecting One participant all cell classes your classification results are shown as a total number of cells per cell class compared to the reference classification Fach cell class 1s shown with a top and bottom bar The top bar dark grey shows the total number of cells classified to this class by the reference The bottom bar shows the total number of cells classified to this class by
29. come James You have the following tests in your account 4 Stevens Logout 2 test number six Close Slides to verify 7 CD Mete i Slide 1 double dlick slide to verify Slide 2 Slide 3 Slide 4 Slide 5 Slide 6 Slide 7 Slide 1 Slide comment add comments Fach test contains one or more slides that need to be verified and signed in order to complete the test 36 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency E e PARTICIPANT 3 2 2 2 Slide States Unsigned slide The verification of this slide is not yet Slide 1 complete and the slide has not yet been signed Signed slide Slide 1 Vv Verification of this slide is complete and the slide has been signed Note To complete a test all slides must be signed 3 2 2 3 Slide Information Click on a slide to obtain more information about that slide at the bottom of the page Slide Comment 1 Click Add comment 2 Add or edit the comment 3 Click Save to save the change or Cancel to return the comment to its previous state Note Slide comments are visible to the Examiner Additional Slide Data Additional slide data is sometimes provided and gives information to help correctly classify and characterize the cells Click on the link to open the file It will open in a program suitable for that file type 3 2 3 Verifying Slides 3 2 3 1 Opening a Slide for Verification In the
30. d Slide note wv e Contains WBC RBC 12142 2010 09 10 KLL PN Double click to verify gt EQ Slide note not visible to participants 1 1314Z 2010 09 10 KLL BNi rrr 151Z 2010 09 10 Blaster DN 1 2010 09 29 KLL BNi 100929 P6 HZ 2010 12 29 Normal IMa 110208 ES Patient information ONVZ 2010 12 29 Normal IMa 110208 mula didclinicenatoa 081 Z 2010 12 29 Normal IMa 110208 de included in texts Tala Slides with body fluid Side ID Analyzed Slide note vie m 4064782 2012 06 12 4064896 2012 06 12 A f Additional slide data 4064577 2012 06 12 A cal nin fose 4064710 2012 06 12 es 4064732 2012 06 12 A 4064885 2012 06 12 4065317 2012 06 12 A Upload slides cA Get the Transfer Tool 1 Slide list 2 Detailed view Navigate to the SLIDES tab to e Upload slides to your CellaVision Proficiency Software account e View and manage uploaded slides e Verify slides Slides are automatically grouped into distinct slide lists 1 according to their sample type Detailed View Click on a slide in a slide list s to view the detailed view 2 of that slide 2 3 3 Managing Slides 2 3 3 1 Slide States wp Empty field Slide has not been marked as done but all cells are classified Iw Slide is marked as done optional step TA Slide contains unclassified cells User s Manual PM 10509 2013 03 04 21 CELLAVISION CellaVision Proficiency Software opes foo EX
31. difference to reference Bi 2 degree difference to reference KS 3 degree difference to reference Examiner Majority User 1 User 2 User 3 User 4 User 5 User 6 You User 8 User 9 0 Tear drop cells Elliptocytosis Spherocytosis Sickle cells Helmet cells Schistocytosis lt Acanthocytosis Echinocytosis Target cells Stomatocytosis Anisocytosis Microcytosis Macrocytosis Poikilocytosis Parasites Howell Jolly Pappenheimer Basophilic stippling Compare to Polychromasia Examiner Majority Hypochromasia 10 OO OO 00 00 00 0 OO 0 0 OO CO 10000002 02 020200 OO OO CO CO Oo EFFE 1 GO 09 0 GO OG 020202 02020202 00002020 1 O e e E E 00 000000 00000230 2 Ge e e e e e e e e e Ce e e e E E E OS 1 GO 020002 0202 000202 00002020 1 O e e 3 DD 000 00000000 Oe OD OO 1 Ge o e e e e e e e e e e Ce e e e e E E E 1 e OO GO 909 0202 000202 00002020 1 E 5 5 3 Oe Oe e e E e Ou E OO Oe Oe CO Oe CO E Click the RBC characteristics tab to see the results of RBC characterization Switch between Examiner and Majority in the compare to box to change the reference characterization The table shows the Examiner Majority and Participant s characterization grades for the different morphologies Cells with an orange background color indicate where the characterization differs from the reference The stronger the orange color the greater the deviation Click Show RBC image to bring
32. e tabs When a slide has no RBC only the WBC tab is shown and vice versa Collapse Expand Click on the arrow to collapse expand 2 the side panel Main View The main view 3 shows either the WBC images of the currently selected classes or the RBC image User s Manual PM 10509 2013 03 04 23 CELLAVISION CellaVision Proficiency Software EXAMINER 2 3 4 3 Adjust Image Brightness and Color 1 Click 2 2 Adjust the sliders 3 Click Save Clicking Reset will restore the default settings Adjust brightness and color Cancel 2 3 4 4 White Blood Cell Classification General Workflow Click on the WBC tab 2 Click on a WBC class in the side panel to show a gallery of all cells classified to that class in the main view When using the two class layout left click on a class to open it in the left gallery and right click on a class to open it in the right gallery Use the drop down menu in an open WBC class gallery to switch between classes 3 If necessary re classify cells by dragging and dropping them from one open class gallery to another or from an open class gallery to a class in the side panel Select multiple cells at once by pressing CTRL or SHIFT Note It is not possible to re classify cells from slides that are locked Note It is possible to add user defined WBC classes See also 2 5 3 Managing User defined Classes for more information 24 PM 10509 2013 03 04 User s Manual
33. eSNSISN Morphology is present at a low level Morphology is present at a moderate level Morphology is present at a high level Using the Ruler In the top left hand corner of the RBC image there 1s a cross shaped ruler that represents 14 um across Click on the ruler and drag it to the desired area in the RBC image The ruler automatically scales up or down according to the zoom level of the RBC image po Gg 09050 Da Oda O A 3 2 4 Signing Slides Signing a slide indicates that the verification of that slide is completed ada 1 Double click on a slide to open it for verification 2 Classify all WBC s until there are no cells left in the unidentified class and characterize the RBC image The Sign slide button activates 3 Click Sign slide Signed slides are indicated in the test overview page by a check mark See also 3 2 2 2 Slide States for more information Note Once a slide has been signed it is no longer possible to change verification 3 3 VIEWING TEST RESULTS 3 3 1 Definitions 3 3 1 1 Examiner result The Examiner result is the WBC classification and or RBC characterization performed by the Examiner 3 3 1 2 Majority result For WBC it is the cell class assigned to a particular cell by the majority of Participants For RBC it is the characterization grade chosen by the majority of Participants 42 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency PARTIC
34. eement The WBC Agreement in the Summary of results tab is the percentage of cells where the participant s classification agrees with the reference classification for the selected orders in the test case Example Assume that there are 200 cells in the selected orders and that the participant s classification agrees with the reference for 165 of the 200 cells The WBC Agreement is now calculated as 165 100 82 5 5092 100 eil Mean The mean average arithmetic mean is calculated by dividing the sum of the observations with the number of observations RBC Agreement The RBC agreement measure for each Participant is calculated as follows For all morphologies and all selected orders the maximum difference from the A me ref ref 0 if ref gt 0 or ind gt 0 0DSmax reference is calculated as 0 otherwise where efx is the reference characterization and 9 is the Participant s characterization of the morphology Then the total maximum difference from di b the reference is the sum of all the differences YI max 2 max For all slides and morphologies the absolute difference between the Participants and the indgige lindy refa diotal gt ind ait reference 1s calculated as The sum of these differences is calculated n The RBC agreement is now calculated as Aa 1 te 100 if dif fnax gt 0 R r 0 max 100 otherwise User s Manual PM 10509 2013 03 04 55 CELLAVISION
35. el to see how the WBC classification results compare between each individual Participant and a chosen reference Change the Participant or the reference using the drop down menus in the Compare 3 box The first two columns in the Comparison results 4 table show the number of cells the two selected individuals have classified to each class The third column shows the number of cells where the classification differs between the two individuals Sort WBC classes with the most disagreements to the top of the list by clicking on the table headers in the Comparison results 4 table Click on a cell class name to show cell images with disagreements in the main view 2 The two differing classifications are shown above the cell image Use the arrow buttons underneath the cell image or the arrow keys on the keyboard to go through all disagreements of that class Cell comments if available are shown underneath the cell image Showing Examiner Cells Click Open Cell Class to show all cells for that slide that were classified to this class by the Examiner Use the drop down menu to change to a different class User s Manual PM 10509 2013 03 04 17 CELLAVISION CellaVision Proficiency Software EXAMINER Note Examiner cells are not to be confused with WBC reference libraries Cells from WBC reference libraries can be compiled from many different slides and serve as examples for their particular cell class see Create WBC
36. er A Participant cannot access these parts of the program GETTING STARTED Creating a Free Trial Account 1 Open an internet browser window and go to https www cellavision proficiency software com 2 Click the Create a free trial account link O Create a trial account 4 Get started with a free 3 month trial account It is possible to upgrade to a full account at any time First name Last name Email address _ accept CellaVision s Terms amp Conditions CellaVision s Terms amp Conditions Return to login page 3 Fill out the form and accept CellaVisions Terms amp Conditions 4 Click Create account The trial account will be active for 90 days during which time it is possible to view the results of completed example tests add up to two Participants and create tests using example slides Requesting a Full Account A full account requires the purchase of a CellaVision Proficiency Software license Find out more at http www cellavision proficiency com licenses Logging in 1 Open an internet browser window and go to https www cellavision proficiency software com O Log in a CellaVision Proficiency Software Password Forgot password Log in New user Create a free trial account CELLAVISION PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency EXAMINER User s Manual Software 2 Enter your email username and password
37. g 1 and Completed 2 tests set by the examiner 3 2 1 1 Test Completion Dates Test completion dates 3 are shown as the second date next to the test name If a completion date is given a test must be completed before that date 3 2 1 2 Test Comments Test comments 4 are indicated by the ES comment icon next to a test Move the mouse over the test comment icon to see the comment 3 2 1 3 Results Status x Hourgass Icon before a completed test indicates that the test results 5 are not yet available E Early access icon before a completed test indicates that results are available but not all Participants in the test group have yet completed the test Incomplete test results from fellow Participants will not be shown User s Manual PM 10509 2013 03 04 35 CELLAVISION E SS PARTICIPANT CellaVision Proficiency Software 3 2 1 4 Account Settings 1 Click on the user name in the top right corner The Account settings 6 window opens 2 Click on the text box next to Email to edit your email address 3 Click on Change next to Password to customize your password 4 Select the language for the user interface using the drop down menus 5 Click Save to save your changes Account settings Name James Stevens Email james stevens lab coml Password Change Save Cancel 3 2 2 Viewing a Test 3 2 2 1 Opening a Test Double click on an ongoing test CELLAVISION de TESTS wel
38. ge using the drop down menu Account information Nowe Tae TT Email _examiner organization com Password Change Language English English r License The License box contains information about license type number renewal date and the organization name User s Manual PM 10509 2013 03 04 31 CELLAVISION CellaVision Proficiency SE Software 2 5 2 1 Upgrading the License Click the Read more about how to upgrade licenses link for more information 2 5 3 Managing User defined Classes Classes Peripheral blood O Body fluid Manage user defined classes Create old cells D Smear cells 1 In the Classes box select either Peripheral blood or Body fluid samples 2 Under Manage user defined classes click on either the WBC non WBC or RBC tab 3 Enter the name of the new class and click Create Editing User defined Classes 1 Click on the class 2 Edit the class name 3 Click Save Deleting User defined Classes Click Ei Delete next to the class to delete it All cells of a deleted class are automatically moved to the Unidentified class and need to be re classified Affected slides will be marked with A in the SLIDES tab Note User defined cell classes that is used in a test cannot be deleted 32 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Software E E 2 6 TROUBLESHOOTING 2 6 1 No Slides Shown in
39. greed on the classification The orange part indicates where they disagreed Change cell class using the drop down menu in the Display box 3 3 6 WBC Cells Click the WBC cells tab to see the results of WBC classification on a cell level User s Manual PM 10509 2013 03 04 47 CELLAVISION CellaVision Proficiency PARTICIPANT E 3 3 6 1 View Classifications CELLAVISION de TESTS Results view Browse test results amp J Stevens Logout In Results forrbc 2 Close Summary WBC WE RBC 1 Show data for slides e Slide 2 Q Compare Cell classifications Slide 1004M E Fiter WBC cells View classifications 1 slide Monocyte 8 Lymphocyte variant form serer cell classifications Slide 1 Cell class Examiner Monocyte Open cell class Examiner Monocyte You Monocyte User 1 Monocyte User 2 Monocyte User 3 Monocyte 4 1 of 108 b User 4 Monocyte User 5 Monocyte User 6 Monocyte User 8 Lymphocyte variant form User 9 Monocyte J 2 1 View classifications 2 Main view 3 Cell classifications 4 Individual cell classifications Click on the View classifications 1 tab in the side panel to see which WBCs caused the most classification disagreements All cells from the currently selected slides are compiled and shown in the main view 2 starting with the cell that has the highest number of disagreements The total number of cells in the current
40. ieri 11 reference cell ce 26 reference cclls iii 9 Reference Libraries 9 18 26 1 0 sssesssseresseserersrssrsssossosesesaresserne 27 S slide data 9 Slide Bh ee 9 22 SO ER 5 10 DIO EE 5 10 33 T Test COMMENT Luci 9 Test Conclusions 19 Test Durapon iii 8 Test End Date 8 11 test PES 6 Test results rici 42 Transfer Tool 19 Tras EE 26 Trash class 26 Trial Account 2 U Under Construcnon 4 User defined Classes 24 32 W WBC Agreement 11 WBC Cells errorroronornornernernernersnenn 15 WBC Classes 14 Work flow cccceccsecescescesceecs 53 54 User s Manual CE CELLAVISION CellaVision AB Ideon Science Park SE 223 70 Lund Sweden Phone 46 0 46 286 44 00 Fax 46 0 46 286 44 70 info cellavision com www cellavision com PM 10509 2013 03 04
41. ipants who have completed the test will be able to view their results even before the test is completed by the entire group Early access results only show that particular Participants results compared to the Examiner results No incomplete results from fellow Participants are shown Add a comment about the test Test comments are visible to all Participants taking this test PM 10509 2013 03 04 CELLAVISION CellaVision Proficiency Software EXAMINER 2 2 3 Monitoring Ongoing Tests CELLAVISION A Kapferer Log out 2 1 Progress of Test 3 Hodgkins Lymphoma A Close O 0 5 participants have completed this test Endtestnow 1 Progress bar 2 Progress grid 3 Sort 2 2 3 1 View an Ongoing Test Double click on an ongoing test in the TESTS tab to view its progress Progress Bar The progress bar 1 indicates how many Participants have completed the test Participants are only classed as having completed the test once they have verified and signed all slides Progress Grid The progress grid 2 shows which slides each individual Participant has completed Participants are shown on the y axis and slides on the x axis of the progress grid Sorting Choose to sort 3 the progress grid on names completed or not completed 2 2 3 2 Stop an Ongoing Test Stop an ongoing test at any time by clicking End test now This will immediately close the test for all Participant
42. lable Participants list Click on the column headers to sort the list Click Y to filter the list on Lab Region or Country If no participants are shown click on the PARTICIPANTS tab and add Participants see also 2 6 3 No Participants Shown in Wizard Step 2 Only available in the Enterprise version CELLAVISION Tame SLIDES PARTICIPANTS SETTINGS AT User Log ot 9 Basic iaat Step 1 Step 2 Step 3 Step 4 Select slides Select the participants Select preferences Review and finish Available participants Selected participants First name Last name Username Email Lab Y Region Y Country Y First name Last name Username Email Mary Johanson cbbc805f Paul Nelson 8b47f562 Lee Lewis af2f2ef7 f60 David Wilson df5cf557 13b Anna Smith 07088cc1 Back Exit Next 5 Click to add the selected participants to the test and then click Next 6 Choose the test preferences see 2 2 2 1 Test Settings for more information and then click Next 6 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Software E CELLAVISION arme iaaa Basic test Select preferences Decide what participants can see J W Show patient information Show additional slide data Make reference cells available Give early access to results lt Bak J Eit We 7 Review the test to ensure that the correct slides and Partici
43. llapse expand 2 the side panel 3 3 3 3 Slide list The slides list 3 shows all slides included in the test Include and exclude slides from the results by clicking on the slide Hold down CTRL or SHIFT whilst clicking to select multiple slides Only results from selected slides i e highlighted in blue are presented 3 3 3 4 Slide comments If one or more slide comments 4 exist for a slide the comment icon P will be shown next to the slide in the slide list Click on the comment icon to open a window with all comments 3 3 3 5 Slide counter The slide counter 5 shows how many slides are currently selected and contributing to the shown results 3 3 4 Test Summary CELLAVISION de TESTS results view Browse test results Ai Stevens Logout 2 In Results for be Close Summar WBC RBC Show data for slides E Filter All ary 1 side Legendi e der Sei names 7 Sec SH Lon ege j A Slide 2 hh 9 participants completed these slides Ba User 2 e Compare to Average 9 o Examiner Majority 3 User 3 WBC 4 A Al Agreement percentages A User 4 Average O O RBC 1 Of A WBC RBC e agreement agreement Participants User 1 94 4 98 4 User 2 92 6 100 0 User 3 94 4 100 0 an 98 100 User 4 95 4 100 0 User 6 92 6 100 0 User 8 88 9 100 0 WBC RBC Export data 0 25 50 75 100 H HOA QRo 1 Bar chart 2 Compare to 3 Agreement percentages
44. miner Participants where an email address was provided will also receive an email containing their account information Only available in the Enterprise version Note The number of available Participants that can be added is determined by the license type See 2 5 2 1 Upgrading the License on how to increase Participant seats 2 4 2 2 Add Participants Via CSV File Upload CSV File Guidelines 1 Arrange the column as shown in the table A B c D E SE 1 Firstname Last name Email adress Lab Region Country 2 John Smith john smith company com John s Lab Neverwood Neverland 3 Note The fields Lab Region and Country are only available in the Enterprise version 2 Ensure that the maximum number of free Participant seats is not exceeded 3 Save the file as a csv file User s Manual PM 10509 2013 03 04 29 CELLAVISION CellaVision Proficiency Software EXAMINER 2 4 3 Filtering the Participant List 1 Click Filter Group by Select r Search 2 Choose Group by or Search and then select a category 3 Click OK Only available in the Enterprise version 2 4 4 Managing Participants Click ona Participant in the participant list to focus the detailed view to that Participant 2 4 4 1 Edit Participant Location 1 Click Edit next to Location 2 Enter Lab Region and Country and click OK Only available in the Enterprise version 2 4 4 2 Reset Participant Password 1 Click Rese
45. n showing One participant all cell classes and One cell class all participants in the Display box to show different views of the data One Participant All Cell Classes CELLAVISION Tame SLIDES PARTICIPANTS SETTINGS AA Kapferer Logout 9 In Results for be 2 Close _ Summary WBC WBC 1 RBC of results cells characteristics Show data for slides Slide 1 1004M WBC classes Total numbers 1 slide Slide 2 1005M Paul au DI Examiner result EI Num classification agrees wit examiner results E Number of cells where classification disagrees with Segmented neutrophil ber of cells where Lymphocyte examiner results Monocyte Compare to Band neutrophi Examiner Majority and neutrophi Display Smudge cell One participant all cell classes Paul v Giant One cell class all participants thrombocyte Basophil Eosinophil Lymphocyte variant form o A n LA N amp CO n CH When selecting One participant all cell classes the selected Participant s classification results are shown as a total number of cells per cell class compared to the reference classification Each cell class 1s shown with a top and bottom bar The top bar dark grey shows the total number of cells classified to this class by the reference The bottom bar shows the total number of cells classified to this class by the Participant The blue part of the bar indicates the number of
46. nt percentages 4 Infographics Click the Summary of results tab to see an overview of how Participants performed in the test Bar Chart The bar chart 1 shows the average WBC and RBC agreement for each Participant displayed as percentage values The higher the agreement the better a Participant is considered to have performed in the test Switch between using either the Examiner or Majority result as a reference for the agreement using the radio buttons in the Compare to 2 box The Agreement percentages 3 box shows the values used for drawing the bars in the bar chart Sort the bar chart using the drop down menu Filter the bar chart using the drop down menus Only available in the Enterprise version User s Manual PM 10509 2013 03 04 13 CELLAVISION T SS gt ON VIN ine CellaVision Proficiency Software Infographics The infographics 4 displays the following key metrics of the test e Total Participant number e Average WBC and RBC agreement for all Participants compared to the reference selected in the Compare to box Examiner or Majority result e Highest and lowest WBC and RBC agreements compared to the reference selected in the Compare to box Examiner or Majority result 2 2 4 5 WBC Classes Click the WBC classes tab to see the results of WBC classification on a class level Switch between Examiner and Majority in the Compare to box to change the reference classifications Switch betwee
47. oftware Examiner and Participants The Examiner performs the testing and education by creating and managing Participants correct classifications and tests The Examiner can view the results of all Participants Participants can view their own results and compare them to the correct result the majority result or anonymized fellow Participant results About this User s Manual This User s Manual will guide you step by step through the operation of the CellaVision Proficiency Software also referred to as the software aiming for an efficient way to give you a good understanding and knowledge of the program and its features References are made to appendices providing additional information Some self explaining dialogs have been left out in the text Typographical convention Names of keys and on screen objects are presented in bold e g click Print Help A help button is located in the top right hand corner Click it to open a window containing links to the following help resources e Online version of this manual e Support community e FAQ s The Help window can be opened or closed at any time and does not affect the current state of work Additional resources can be found at http www cellavision proficiency com User s Manual PM 10509 2013 03 04 1 CELLAVISION EXAMINER CellaVision Proficiency Software 2 2 1 2 1 1 EXAMINER The following chapters are intended for the Examin
48. ons and or re classifying WBCs Reference cells Cells displaying typical characteristics for their class Reference results The Examiner result or the majority result depending on the context which it appears in Signing Finally confirming analysis results before locking and reporting them Unidentified A cell class for cells and objects which are not yet classified User s Manual PM 10509 2013 03 04 57 CELLAVISION Index CellaVision Proficiency 58 Software A access tO resultS Lie 9 Available Participants 6 Available slides 5 C Cell Market 0 ccceccescoeeeess 40 classification disagreements 16 Collapnseiexpand 12 Compare Individuals 17 COMPIE 5 CSV FILE 29 D databases ccccsccsccescecceccescesceecs 19 distribute the test 7 E End test circeo iene iene 10 EXAMINED ice rien rie scenes l Examiner result ve 11 F Full Account 2 L Lab Supervisor 30 ITT ia 2 os 2 34 M Majority result 11 N New RE 5 notification email 7 O ONSON EE DATORE 4 PM 10509 2013 03 04 P PPs l Password 3 Patient Information 9 22 Progress EE gece 10 Progress GU avsa 10 R RBC Agreement 11 RBC Characteristics 18 reference
49. pants were added and the correct preferences were set CELLAVISION Basic test Review test Test ends Tuesday Jan 29 2013 J v Show patient information V Give early access to results E No test comment 8 Click Finish to distribute the test to all Participants A notification email will be sent to all Participants who have an email address associated with their account Participants with no email address need to be notified manually User s Manual PM 10509 2013 03 04 7 CELLAVISION CellaVision Proficiency Software EXAMINER 2 2 2 1 Test Settings CELLAVISION da Tame SLIDES A PARTICIPANTS SETTINGS T User Logout 9 Basic test Step 1 Step 2 Step 3 Step 4 Select slides Select the participants Select preferences Review and finish Select preferences Set test duration Automatically close this teston 1 29 13 E Do not set an automatic completion date Va Decide what participants can see wl Show patient information how additional slide data EI s Make reference cells available V Give early access to results ES Write a test comment Back Exit Next Test Duration Automatically close Specify an end date using the drop down menu or this test on calendar At the end of the chosen day the test will close automatically independent of the Participants progress and be flagged as completed Test end dates will be visible to the Participants Note
50. play box 2 2 4 6 WBC Cells Click the WBC cells tab to see the results of WBC classification on a cell level User s Manual PM 10509 2013 03 04 15 CELLAVISION CellaVision Proficiency EXAMINER Software View Classifications CELLAVISION Tia SLIDES A PARTICIPANTS 3 SETTINGS AA Kapferer Logout 9 Il Results for be Close Summary WBC WE RBC of results Classes cells characterization WBC cells View classifications 1 slide Show data for slides Slide 1 1004M e Slide 2 1005M G Cell classifications Slide 1004M Examiner Smudge cell Smudge cell 5 Lymphocyte Individual cell classifications Slide 1004M Cell class Examiner Smudge cell Paul Smudge cell David Lymphocyte Anna Lymphocyte Mary Smudge cell Linda Smudge cell John Lymphocyte Karen Smudge cell EI 23 of 108 gt Suzan Lymphocyte Laura Smudge cell 2 1 View classifications 2 Main view 3 Cell classifications 4 Individual cell classifications Click on the View classifications 1 tab in the side panel to see which WBCs caused the most classification disagreements All cells from the currently selected slides are compiled and shown in the main view 2 starting with the cell that has the highest number of disagreements The total number of cells in the current list 1s shown in brackets next to the heading All cells above the cell image The Cell classifications 3 box
51. results view Include and exclude slides from the results by clicking on the slide Hold down CTRL or SHIFT whilst clicking to select multiple slides Only results from selected slides i e highlighted in blue are presented Slide Comments If one or more slide comments 4 exist for a slide the comment icon will be shown next to the slide in the slide list Click on the comment icon to open a window with all comments 12 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Software EE Slide Counter The slide counter 5 shows how many slides are currently selected and contributing to the shown results 2 2 4 4 Test Summary CELLAVISION Tim SLIDES AB PARTICIPANTS SETTINGS AA Kapferer Logout 9 Ila Results forme Close Summa WBC WBC RBC asses cell haracteristic 4 Show data for slides Summary slide Legend n Sorted by Names x Slide 1 1004M Slide 2 1005M Anna we 9 1 participants 2 BSS O Average e 4 o y d Examiner ee EE sa Rec 100 aura RBC O Agreement percentages Participants WBC agr RBC agr Linda Paul 94 4 98 4 David 926 100 0 Anna 94 4 100 0 Mary 98 100 Mary 95 4 100 0 Linda John Karen Suzan Sc g BC RBC e 89 98 Paul Laura Suzan ees e o o o CH S S Ee 25 50 75 100 9 1 Bar chart 2 Compare to 3 Agreeme
52. rint Ca me CE venom Lab Supervisor 52 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Recommended Workflow Software Appendix B Recommended Workflow Confirm correct classification of slides Examiner Go to the SLIDES tab Double click on a slide Verify WBC classification Click the RBC tab Verify RBC classification Mark the slide as done Click Close Repeat with the next slide in the list as needed Create a New Test Go to the TESTS tab Enter a new test name and click Create The wizard opens Follow each step of the wizard and click Next Review the test and click Finish Create Participants Examiner Go to the PARTICIPANTS tab Click Add participant for manual adding or click Import CSV file Add participants and click Create User s Manual PM 10509 2013 03 04 53 CELLAVISION CellaVision Proficiency Recommended Workflow Software Take a test Participant Go to the TESTS tab and click on an ongoing test Double click on a slide Classify all WBCs Click on the RBC tab if avaliable and characterize the RBC morphology Sign the slide by clicking Sign slide and click Close View Results Go to the TESTS tab Double click on a Completed test 54 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Calculations Software Appendix C Calculations WBC Agr
53. s independent of their progress Once stopped the test is flagged as completed and its results will be available for viewing See also 2 2 4 Viewing Test Results on how to access test results 10 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency EXAMINER Software Note Closing a test before all Participants have verified all slides will reduce the data available for the results statistics Any slide a Participant has not completed will be excluded from the results for that particular Participant Note Once a test has been stopped it cannot be returned to an ongoing status 2 2 3 3 Extending the Test End Date 1 Go to the TESTS tab and click on an ongoing test 2 In the detailed view of that test click on the date underneath Test end date 3 Enter a new date and click Save Note The test end date can never be set to a more recent date than the previous end date It is only possible to extend the test end date if a test end date has been set from the beginning 2 2 4 Viewing Test Results 2 2 4 1 Definitions Examiner Result The Examiner result is the WBC classification and or RBC characterization performed by the Examiner Majority Result For WBC it is the cell class assigned to a particular cell by the majority of Participants For RBC it is the characterization grade chosen by the majority of Participants If the majority cannot be decided unanimously one of the cell cla
54. s and dragging cells from the Trash class to another cell class Create WBC Reference Libraries 1 Right click on the cell that should be used as a reference for that class 2 Select Add as reference cell from the right click menu To view reference libraries 1 Switch to either the one class or two class layout 2 Open the cell class of Interest 3 Click Reference cells The reference cell gallery for that class will open as a separate gallery To delete cells from reference libraries 1 Right click on a cell 2 Select Delete from the menu Note Reference libraries will be available to Participants if specified during test creation Only reference cells created before the test is created will be shown to Participants See 2 2 2 1 Test Settings for more information 2 3 4 5 Red Blood Cell Characterization CELLAVISION rests ra SLIDES PARTICIPANTS Q SETTINGS AA Kapferer Logout 2 gt 121 Z 189 Markasdone rw Close WBC 0123 Polychromasia Hypochromasia Anisocytosis Microcytosis Macrocytosis Poikilocytoss Target cells Schistocytoss Helmet cells Sickle cels Spherocytosis Elliptocytosis Ovalocytosis Tear drop cells Stomatocytosis Acanthocytosis Echinocytosis Howell Joly
55. shows the cell classes and number of total classifications to each cell class for the currently shown cell It also shows which slide the cell originates from The Individual cell classifications 4 box shows how the Examiner and each Participant classified the cell Use the arrow buttons underneath the cell or the arrow keys on the keyboard to go through all the cells The cells are sorted so that the cells with highest number of different classifications are shown first 16 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Software EGNER Compare Individuals CELLAVISION Tame SLIDES PARTICIPANTS XX SETTINGS AA Kapferer Logout 9 Ih Results for pe Close Summary WBC RBC i ill of results classes characteristics Show data for slides Slide 1 1004M Slide 2 1005M WBC cells Compare individuals 1 slide Segmented neutrophil Disagreements 4 Examiner Band neutrophil em Paul Segmented neutrophil N Compare e 4 Examiner 2 Paul Comparison results Open cell class Examiner Paul Lymphocyte 28 30 Monocyte 10 10 Lymphocyt 2 0 Band T 6 Smudge cell Giant Eosinophil Basophil TOTAL 10 lt 10f4 gt A e e e t Me MS 1 1 1 1 8 Q 1 CH sch 1 10 2 l 2 Main view Compare individuals 3 Compare 4 Comparison results Click on the Compare individuals 1 tab in the side pan
56. sses characterizations is at random used as majority Reference The reference is either the Examiner result or Majority result depending on the selection made by the viewer WBC Agreement WBC Agreement shows how well the Participant s classification agrees percentage with the reference RBC Agreement The RBC Agreement shows how well the Participant s characterization agrees percentage with the reference 2 2 4 2 Opening a Completed Test In the TESTS tab double click on a completed test to open the results view User s Manual PM 10509 2013 03 04 11 CELLAVISION CellaVision Proficiency EXAMINER Software 2 2 4 3 The Results View 1 Is Results for Test 1 Basic Peripheral Blood K Summa WBC WBC 1 RBC classes cells 4 characteristics e Show data for slides Summa 1 slide Slide 1 1004M Slide 2 1005M 1 Summary of results 2 Collapse Expand 3 Slide list 4 Slide comments 5 Slide counter Results View Navigation In the results view the test results are automatically compiled to show e A summary of results e WBC classification statistics on a class level e WBC classification statistics on a cell level e RBC characterization statistics Click the results view navigation tabs to browse the results Collapse Expand Click on the arrow to collapse expand 2 the side panel Slide List The slides list 3 shows all slides in the test that contribute to the current
57. t next to Password 2 A new password is automatically generated and emailed to the Examiner Note Passwords can only be reset for Participants that have no email address associated with their account Participants with email addresses can reset their password from within their own account 2 4 5 Setting Lab Supervisor Privileges 1 In the PARTICIPANTS tab click on any Participant to focus the detailed view to that Participant 2 Click Set Lab Supervisor Privileges 3 Choose Lab Region or Country to allow the Lab Supervisor to view test results with Participant names within the selected category 30 PM 10509 2013 03 04 User s Manual 2 4 6 2 5 2 5 1 2 5 2 CELLAVISION CellaVision Proficiency Software Set Lab Supervisor privileges View test results with Participant names within the selected category EXAMINER None Lab Region Country Only available in the Enterprise version Deleting Participants 1 In the PARTICIPANTS tab click on any Participant to focus the detailed view to that Participant 2 Click Delete to delete the Participant SETTINGS Click on the SETTINGS tab to manage your account and customize the workspace Editing Account Information 1 In the Account information box click on the current name or email address 2 Edit the information and click Save 3 Customize the password by clicking Change and entering a new password 4 Customize the langua
58. test overview page double click on a slide to verify it User s Manual PM 10509 2013 03 04 37 CELLAVISION CellaVision Proficiency Software PARTICIPANT 3 2 3 2 The Verification View 1 4 CELLAVISION de mg Verification of Slide 5 ac Potter Log out Slide5 Q ss J e Sign Slide Close I CS PE E 100 Unidentified tb Band neutrophil 7 Band neutrophil Segmented neutrophil be r Eosinophil Basophil Lymphocyte Monocyte Promyelocyte Myelocyte Metamyelocyte Promonocyte Prolymphocyte Blast This cell class is empty Lymphocyte variant form Plasma cell x Add cell images by dragging them here Large granular lymphocyte Hairy cell S zary cell Erythroblast Giant thrombocyte Thrombocyte aggregation Megakaryocyte Smudge cell Artefact 1 WBC RBC navigation 2 Collapse Expand 3 Main view 4 Sign slide WBC RBC Navigation Switch between WBC and RBC 1 verification using the tabs When a slide has no RBCs only the WBC tab is shown and vice versa Collapse Expand Click on the arrow to collapse expand 2 the side panel Main View The main view 3 shows either the WBC images of the currently selected classes or the RBC image Sign Slide Button Click Sign Slide 4 to sign the slide when verification is complete 3 2 3 3 Adjust Image Brightness and Color 1 Click 2 Adjust the sliders 3 Click Save Clicking
59. the desired area in the RBC image The ruler automatically scales up or down according to the zoom level of the RBC image O GO wO 9 3500 9 98 OOOO ep w E en Ga CH een 2 3 5 Adding or Editing Slide Comments AIN 1 In the SLIDES tab double click on a slide to open the verification view 2 Click ld Comment next to the slide identifier 3 Add or edit the comment and click Save User s Manual PM 10509 2013 03 04 27 CELLAVISION SE SS EXAMINER CellaVision Proficiency Software 2 3 6 Marking Slides as Done To remember which slides are ready to be used in tests they can be marked with a check mark by clicking in the verification view Note This can only be done if there are no unidentified cells 2 3 7 Deleting Slides 1 In the SLIDES tab click on any slide to bring up the slide information in the detailed view 2 Click Ei Delete to delete the slide Note Slides that are currently included in tests are locked and cannot be deleted 2 4 ADDING AND MANAGING PARTICIPANTS 2 4 1 The PARTICIPANTS Tab CELLAVISION 1 R PARTICIPANTS SETTINGS 2 AA Kapferer Logout 9 BX Participants James Stevens Firstname Last name Username mail Lab Y Region Y Coun oing tests Completed tests Firstname a Last name Usemame X h ty Quaona E A Username 329da87b 6b7 d i Carl Svensson 97d7517d e00 Berlin Medical Lab Berlin Germany 0 3 Name James
60. up the RBC image Click outside of the image to close it Note RBC characterization results can only be viewed for one slide at a time 3 3 8 Test Conclusions Test conclusions are reports created externally by the Examiner and may be attached to the result of a test 1 Double click on a completed test in the TESTS tab to open the results 2 A button next to the test name indicates that a test conclusion 1s available 3 Click on the button The test conclusion will open in a program that supports that file type 50 PM 10509 2013 03 04 User s Manual CELLAVISION CellaVision Proficiency Soi Buttons and Symbols Appendix A Buttons and Symbols A 1 General Place the mouse pointer over any button or symbol to view a short explanation in a tooltip A 2 Buttons FT Change the WBC gallery layout Zoom In sare Adjust image color and brightness Show the cell markers Open the attached test conclusion rieti Show next cell image 4 Show previous cell image 2 0 previous cell image Lei Set Lab Supervisor privileges User s Manual PM 10509 2013 03 04 51 CELLAVISION CellaVision Proficiency Buttons and Symbols EE A 3 Symbols Additional slide data Slide contains unclassified cells general warning symbol Slide is locked Verification cannot be changed and the slide cannot be deleted Examiner Slide is marked as done Participant Slide 1s signed Comment P
61. vigate to the file 5 Click OK Deleting a Test Conclusion Click W Delete next to the test conclusion link 2 2 Deleting Tests 1 In the TESTS tab click on any test to bring up the test information in the detailed view 2 Click W Delete to delete the test Note Deleting an ongoing or completed test will remove it from all associated Participant accounts Deleting a completed test will also delete its results 2 3 VERIFYING AND MANAGING SLIDES 2 3 1 Uploading Slides The CellaVision Proficiency Software accepts slides from the following CellaVision databases e CellaVision Processing Database e CellaVision Competency Software Database Step 1 Preparation 1 Go to the computer running the CellaVision Remote Review Software and open an internet browser window 2 Navigate to https www cellavision proficiency software com 3 Log in to your CellaVision Proficiency Software account 4 Click on the SLIDES tab 5 Click Get the Transfer Tool 6 Install the Transfer Tool User s Manual PM 10509 2013 03 04 19 CELLAVISION SE SS EXAMINER CellaVision Proficiency Software Note Administrator privileges are required to install the Transfer Tool Note Steps 2 6 can be skipped if the Transfer Tool has previously been installed on the computer Step 2 Export 1 Start the Transfer Tool and select the database containing the slides orders to transfer 2 Log in to that database

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