Install Fraction collector F9-R
Contents
1. UNICORN The system will restart automatically when the configuration has been changed and the system can be reconnected Follow the instructions below to update the system in UNICORN Step Action 1 e Onthe Tools menu in the Administration module click System Proper ties or click the System Properties icon to open the dialog Result The System Properties dialog is displayed e Select the system of interest in the System Properties dialog Note Only active systems can be edited Result The Edit dialog is displayed The KTA pure dialog is shown in the example below Edit LEE204 Instrument configuration Instrument server Instrument serial no Connect by Component types AKTA pure 1 0 0 11 HCE ZC107DKT2 Rename LEE204 Fixed IP address Instrument serial no Valves Monitors and sensors her Fraction collectors Core components always present 10 1 1 1 Connection Test Component selection Property Fraction collector F9 R Fraction collector 2 F9 R AKTA avant and AKTA pure Instructions 29 0214 62 AC 9 Step Action 2 Select Fraction collectors from the Component types list Result All available fraction collectors are shown in the Component selection list e Click the checkbox to select the added fraction collector Note Instrument modules are referred to as Components in UNICORN 3 Click OK to apply t2. then fingertighten the Tubing holder nut This ensures the correct length of the exposed tubing end 6 Re install the tubing holder in the Delivery arm Check that the Tube sensor is in the correct position for the tube size The eluent tubing should be above the center of the collection tube Use the sensor control to position the Tube holder Connection to AKTA pure For Fraction collector F9 R Connect the tubing from the Fraction collector to e port Frac on Outlet valve V9 O V9H O V9 Os or V9H Os For Fraction collector F9 R 2nd Connect the tubing from the second Fraction collector to e port Out 10 on Outlet valve V9 O or V9H O e port Out 1 on Outlet valve V9 Os Connection to AKTA avant For Fraction collector F9 R 2nd Connect the tubing from the second Fraction collector to e port Out 10 on Outlet valve V9 O or V9H O Set the delay volume according to the length of the tubing used according to the instruction above AKTA avant and AKTA pure Instructions 29 0214 62 AC 13 Standard tubing dimensions The table below shows recommended standard tubing dimensions to connect Fraction collector F9 R Tubinglength Tubing diameter mm mm AKTA pure 25 AKTA pure 150 AKTA avant 25 AKTA avant 150 Note Precut standard tubing for AKTA pure are delivered with the fraction collector For AKTA avant cut the tubing according to the table above from the spare tubing delivered with t
3. 00 145 yl Fraction collection Tubing and Delay volumes Tubing Injection valve to column Delay volume pH valve Delay volume Monitor to frac Delay volume Monitor to frac 2 Wash settings Watch parameters Advanced Data collection lt 4 e Select Tubing and Delay Volumes and select the delay volume option of interest Note For KTA avant Delay Volumes are found under Fraction collection e Check the delay volume in the Volume fields and enter a new value if necessary KTA avant and KTA pure Instructions 29 0214 62 AC 11 Connect tubing Follow the instructions in the table below to connect the tubing from AKTA avant and AKTA pure to Fraction collector F9 R or to both Fraction collector F9 R and Fraction collector F9 R 2nd 12 Step Action 1 Lift out the Tubing holder 4 from the Delivery arm 1 000a OAN OSSO Function Delivery arm Tube adjustment cavity Sensor control Tubing holder Tubing holder nut Exposed tubing end Loosen the nut of the Tubing holder Do not remove the Tubing holder nut 5 from the Tubing holder Insert the tubing through the Tubing holder KTA avant and KTA pure Instructions 29 0214 62 AC Step Action Place the Tubing holder with the tubing over the Tube adjustment cavity 2 of the Delivery arm Push the tubing down against the bottom of the Tube adjustment cavity and
4. AKTA avant and AKTA pure Instructions 29 0214 62 AC 3 Illustration of Fraction collector F9 R Function Lock knob Stationary part of delivery arm Delivery arm Tubing connector Tube sensor Collection tubes Tube rack Base unit KTA avant and KTA pure Instructions 29 0214 62 AC Unpacking Follow the instruction below to unpack the Fraction collector and to remove the transport fixations Step Action 1 Open the transportation box 2 Lift off the piece of foam and check the content according to the supplied package list AKTA avant and AKTA pure Instructions 29 0214 62 AC Step Action 3 Lift out Fraction collector F9 R by the Base unit and place it on the bench NOTICE Never lift Fraction collector F9 R by the Delivery arm This may damage the Fraction collector 4 Remove the plastic bag 5 Lift the Delivery arm and gently move the Delivery arm to the outer stop KTA avant and KTA pure Instructions 29 0214 62 AC Step Action 6 Remove the piece of foam from under the Tube rack Installation For information on how to assemble Fraction collector F9 R please refer to the respective instrument User manual and Fraction collector F9 C and F9 R Operating instructions Illustration of Fraction collector F9 R connector panel Part Function Node ID switch UniNet 9 communication and power supply KTA avant and KTA pu
5. Instructions 29 0214 62 AC AKTA avant and AKTA pure Install Fraction collector F9 R Scope Instructions on how to install one or for AKTA pure two optional Fraction collector F9 R 29 0113 62 modules Description Introduction Fraction collector F9 R collects fractions from AKTA avant and AKTA pure purification runs Fraction collector F9 R can be used for e Time or volume based fractionation e Peak fractionation e Combined time or volume based fractionation and peak fractionation It is possible to install up to two Fraction collector F9 R modules when using AKTA pure AKTA avant has a built in fraction collector and it is possible to install one Fraction col lector F9 R as an extra module Note If two fraction collectors are to be used the second one must be configured as Fraction collector F9 R 2nd independent of the first fraction collector type Location AKTA pure The illustration below shows Fraction collector F9 R together with AKTA pure It is recom mended to place Fraction collector F9 R on the left side of the instrument Fraction col lector F9 R can also be placed on a shelf located beneath the system AKTA avant and AKTA pure Instructions 29 0214 62 AC AKTA avant In AKTA avant it is recommended to place Fraction collector F9 R on the right side of the instrument Note Consider the tubing going to Fraction collector F9 R if AKTA avant is turned on its swivel foot
6. he AKTA avant system 14 AKTA avant and AKTA pure Instructions 29 0214 62 AC Page intentionally left blank For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com AKTA GE and GE monogram are trademarks of General Electric Company AKTA and UNICORN are trademarks of General Electric Company or one of its subsidiaries Any use of UNICORN is subject to GE Healthcare Standard Software End User License Agreement for Life Sciences Software Products A copy of this Standard Software End User License Agreementis available on request 2012 2015 General Electric Company All rights reserved First published Sep 2012 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 29 0214 62 AC 04 2015 a30
7. he changes Check Set delay volume When a module has been installed after the UV monitor in the flow path the delay volume has to be adjusted in the System Settings dialog in UNICORN to make sure that the collected fractions correspond to the fractions indicated in the chromatogram Delay volumes can be set for the options Monitor to outlet valve Monitor to frac Monitor to frac 2 and pH valve Depending on the system configuration used different delay volume options will be available for selection in the System Settings dialog The delay volume has to be set for all displayed options Delay volumes for KTA avant and KTA pure modules and standard tubing configura tions are found in the respective User Manual Follow the instructions below to check set the delay volumes Step Action 1 On the System menu in the System Control module click Connect to Sys tems or click the Connect to Systems icon Result The Connect to Systems dialog opens 2 e Select a system e Select Control mode Result The selected instrument can now be controlled by the software 10 AKTA avant and AKTA pure Instructions 29 0214 62 AC Step Action 3 On the System menu click Settings when the system is in state Ready Result The System Settings dialog is displayed The KTA pure dialog is shown in the example below System Settings System 1 Instructions Parameters for Delay volume Monitor to outlet valve Volume 0 100
8. re Instructions 29 0214 62 AC 7 Connect the Fraction collector Connect the UniNet cable between any of the UniNet 9 ports on the back of the instru ment and on the back of the Fraction collector F9 R or Fraction collector F9 R 2nd Make sure that all unused UniNet 9 ports on the instrument are plugged Node ID Node ID is a unit number designation that is used by the instrument to distinguish be tween several units of the same type All standard valves and available optional modules are pre configured to give the desired function However the function of a valve or module can be changed by changing the Node ID Also in a troubleshooting situation it may be useful to check the Node ID of the respective valve or module Note The function of a valve or module is defined by a combination of the module type and its Node ID not by its physical position Check change Node ID The Node ID is set by positioning the arrow of the rotating switch at the back of the Fraction collector Use a screwdriver to set the arrow of the switch to the desired number Step Action 1 Set Node ID to 0 for Fraction collector F9 R and to 1 for Fraction collector F9 R 2nd 2 Connect Fraction collector F9 R and or Fraction collector F9 R 2nd to the instrument 8 KTA avant and KTA pure Instructions 29 0214 62 AC Software configuration When a Fraction collector has been installed the System Properties for the system must be updated in
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