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Stabilizor® T1 Quick Guide

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1. Ex vivo time The time the sample has been outside the living organism Further sample preparations After the stabilization the sample can for example be gt Dissected gt Homogenized for further analysis gt Microtome sectioned for imaging gt Stored frozen for later preparation analysis Stabilizor T1 Quick Guide DPM 0010 2010 Denator www denator com March 2010 Denator Stabilizor and Maintainor are trademarks of Denator AB samples in an experimental series minimizes result variation due to e g degradation Plan your experiment to combine short ex vivo time and careful sample collection Perform sample collection efficiently but without stress Make all preparations before starting sample collection i e prepare Maintainor cards enter data into Stabilizor T1 instrument Perform a mock run before Starting to ensure that everything is prepared before risking any samples Denator AB Arvid Wallgrens Backe 20 SE 413 46 Gothenburg Sweden Phone 46 0 31 412 841 Fax 46 0 31 412 840 order denator com support denator com www denator com denalor
2. Stabilizor T1 Quick Guide Turn on power and insert a USB flash drive 1 Yat System ready after 3 5 min Enter Sample name gt Maintainor card number Select Pb Method Place or centered in Maintainor card fe AE TA a gt Instrument ready W gt Start ex vivo time and immediately collect the sample Place Maintainor card in Stabilizor T 1 Press start Green illumination stabilization finished Remove the USB flash drive with the log file View the log file on any computer with Microsoft Excel Remove Maintainor card The sample is ready for further preparation analysis see back page or long time storage in freezer A Please refer to Stabilizor T1 User Manual for safety instructions and more detailed information genalor Stabilizor T1 Quick Guide Advice for multiple samples gt Similar ex vivo time between Sample stabilization methods gt Structure Preserving For preserving the anatomical structures of the sample Measures sample and adjusts the heating time Fresh Auto For most fresh samples Measures sample and adjusts the heating time Frozen Auto For frozen samples Measures sample and adjusts the heating time includes extra time due to low starting temperature Custom Manual mode where all parameters can be set by the user Maintainor Tissue for tissues up to 7 mm thick

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