How to use the Peloris Dual Retort Rapid Tissue Processor
Contents
1. Ip Pacific Pathology Associates Inc Clinical Anatomic Pathology Histology RL Procedure histo17 01 How to use the Peloris Dual Retort Rapid Tissue Processor Final Approval May 2010 Effective May 2010 Next Review Date May 2012 List all stakeholder s and dates of approval Stakeholder Name s Shelly M Siegel HT Date 8 25 2010 Reviewed X Revised _ Stakeholder Name s Mark Magilner MD Date 8 2710 Reviewed xX Revised _ Stakeholder Name s Shelly Siegel Date 5 3 11 Reviewed X Revised _ Stakeholder Name s Date Reviewed _ Revised L Stakeholder Name s Date Reviewed _ Revised _ Describe briefly the most recent revision made to this policy procedure or protocol amp why New Procedure Purpose Policy Statement This procedure addresses on how to start change reagents and do daily weekly and monthly maintenance on the Peloris Definitions e na POLICY CONTENT The Peloris dual retort rapid tissue processor preserves tissue specimens by fixing in 10 neutral buffered formalin dehydrating in alcohols clearing in xylene then infiltrates with wax This allows tissue to be sectioned stained and ready to be diagnosed by a pathologist STEPS KEY POINTS TO START THE PELORIS Step 1 Load the protocol e Press the SELECT or PROTOCOL button e Choose the protocol SMALL MEDIUM or LARGE e Press LOAD e Pick the retort you are loading A or B Step 2 Start the Run e Pre2. lean sensors with 70 100 alcohol Check reagent wax levels Make sure they are not over or under the MAX line Clean the top surface of the retort lids and handle Clean touch screen WEEKLY Check condensation bottle empty if needed QUARTERLY Change carbon filter JAN APR JUL OCT Clean reagent bottle Equipment Supplies If Applicable Alcohol Xylene graded reagent PPE gloves goggles apron or face mask Funnel Paraplast X tra How to use the Peloris Dual Retort Rapid Tissue Processor Page 2 of 3 Cassette basket Gauze paper towel 10 NB formalin Form Name amp Number or Attachment Name If Applicable Histo17 Author Position Lead Histologist Review Revision Authority Position Not Individual Name Lead Histologist Expert Consultant Position s Not Individual Name s n a References Required for Clinical Documents Peloris Rapid Tissue Processor user manual Peloris alarm and error code manual Is there a Regulatory Requirement Yes No L If yes insert requirement information here Review History No Changes Revision History Note changes in area under header Computer Search Words Policy Procedure or Protocol Cross Reference Information n a How to use the Peloris Dual Retort Rapid Tissue Processor Page 3 of 3
3. ss RUN e Enter the of CASSETTES Press OK e Prompts you to load Press DONE Step 3 Scheduling e Select the endtime to ASAP should read required endtime ASAP The ASAP button will be highlighted in Orange e Press the GREEN START button How to use the Peloris Dual Retort Rapid Tissue Processor Page 1 of 3 REAGENT WAX REPLACEMENT Replace reagent wax when chamber appears striped on the status screen or when warned at protocol start Concentration threshold exceeded for last step Document all reagent wax changes on the equipment maintenance form REAGENT Step 1 Step 2 waste Step 3 DAILY Pull reagent bottle from peloris Empty according to chemical waste management Down the drain or in a labeled waste container to recycle or Push the bottle back into the same station State FULL Press RESET Connect wax waste line hose Insert or rest the hose into container or funnel Open the REAGENT tab then press REMOTE FILL DRAIN Select WAX WASTE and the wax chamber to drain then click DRAIN TO WASTE Monitor drain progress wait until notified of completion Add new wax Clean the wax bath using gauze Add molten paraffin to MAX line Do not over fill Update Station State Open the REAGENT tab then find STATIONS Press the WAX CHAMBER Press the DRY state on the wax chamber that was emptied Press FULL MAINTENANCE Clean gasket seal on retort with 70 100 alcohol Make sure it is free of paraffin C
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