User Manual CxL - Meyer Instruments
Contents
1. 09 This is a special feature provided to compensate the tube length change while changing IPD setting Follow this procedure to obtain optimum parfocality of system to your IPD setting Using the Eye Shades When Wearing Eyeglasses Use with the eye shades in the normal folded down position This will prevent the eyeglasses from being scratched When Not Wearing Eyeglasses Extend the folded eye shades outwards direction of the arrow to prevent extraneous light from entering into your line of vision 9 CxL a Adjusting the Condenser Position and Aperture Iris Diaphragm use is fully illuminated SSS The condenser is most often used in the highest position If the observed x S ls F field of view is not uniform enough it may be improved by lowering the condenser slightly A L0 2 Rotate the condenser height adjustment knob 1 in clockwise direction to move the condenser to the highest position 1 IET Slide the aperture iris diaphragm 2 so that the aperture of the objective in BSS Fig 23 Switching the Objectives Hold and rotate the revolving nosepiece 1 so that the objective to be used is in line above the specimen Always use the ribbed grip to rotate the objective nosepiece Fig 24 oa 20 a Using the 100X Immersion2. immediately PA k CxL 1 c TROUBLESHOOTING GUIDE Under certain conditions performance of the unit may be adversely affected by factors other than defects If problems occur please review the following list and take remedial action as needed It you cannot solve the problem after checking the entire list please contact Labomed for assistance Trouble Cause Remedy 1 Uneven brightness in observation The objective is not engaged in the Engage the objective into position field light path Until the nose turret clicks The condenser is too low Raise up to achieve maximum light The objective eyepiece condenser Clean them thoroughly and or window lens are dirty Clean them thoroughly with lens tissue and alcohol 2 Dust or stains are visible in The eyepiece condenser window lens observation field and or specimen glasses are dirty 3 Observation image has glare The condenser is too low Raise it The condenser iris diaphragm ring is excessively closed Adjust the aperture according to the objective magnification 4 Observation image is hazy The objective is not engaged in the Engage the objective into position or unclear light path until it clicks The objective eyepiece condenser Clean them thoroughly and or specimen glasses are dirty Immersion oil is not used with an Use immersion oil immersion objective Bubbles are present in immersion oil Remove the bubbles by agitation The spe
3. adjustment knob 2 for fine focusing Working Distance WD The WD refers to the distance between each objective and the specimen when precise focus of the specimen is obtained Objective Magnification 4X 10X 40X 100X WD mm 22 10 5 0 56 0 1 18 Adjusting the Interpupillary Distance Fig 22 The inter pupillary distance adjustment consists of regulating the two eyepieces to align with both eyes pupils so that you can observe a single microscopic image through two eyepieces in stereo vision This greatly helps to reduce fatigue and discomfort during observation While looking through the eyepieces move both eyepieces until the left and right fields of view coincide completely The position of index dot indicates the inter pupiliary distance value Note your interpupillary distance so that it can be quickly duplicated Procedure for adjusting the diopter 1 Rotate the right eyepiece to match the markings of your IPD If your IPD is 64 rotate the eyepiece to 64 mark While looking through the right eyepiece with your right eye rotate the coarse and fine adjustment knobs to bring the specimen into focus While looking through the left eyepiece with your left eye rotate only the diopter adjustment ring on the eyepiece until specimen is at its best possible focus D
4. charged with a direct input power supply of 110V 240V AC 50Hz 60Hz This ensures continuous operation even under fluctuating voltages Our Halogen bulb 6V 20W on this instrument has an average life span of up to 2000 hours and LED has an average life span of upto 100 000 hours The CxL comes equipped with a removable N A 1 25 Abbe condenser for brighter illumination levels and an iris diaphragm for better resolution and contrast control CxL SAFETY INFORMATION 1 After the microscope has been used for observation of a specimen containing bacteria clean all parts coming in contact with the specimen to prevent infection E Be sure to remove the specimen before moving this product W n case the specimen is damaged by erroneous operation it is important to clean all surfaces that may have come in contact with the specimen 2 To avoid potential shock hazards and burns when replacing either halogen bulb or LED turn the microscope s main switch to OFF position and disconnect power cord from wall outlet in advance Whenever you replace bulb during use or right after use allow lamp socket and bulb to cool before touching Fig 1 Applicable bulb LED replacement 6V20W Halogen bulb P N CX 013 or LED P N 9135000 901 3 Install microscope on a sturdy level table or bench and avoid any blockage of air vents on the bottom side of the base Do not place microscope on a flexible surface as this could result
5. this will damage the rotary mechanisms When the specimen holder reaches stop position the rotation force of the above knobs become heavy Stop rotating the knob at this time 17 9 CxL Cover glass Slide glass Fig 17 Fig 18 Adjusting the Focus Fig 19 Coverglass This is the glass plate placed on the specimen For optimum optical performance the cover glass thickness which is the distance from its surface to the specimen surface should be 0 17 mm Slide glass This glass plate should ideally have a length of 76 mm width of 26 mm 1 mm and thickness between 0 9 and 1 4mm Specimen holder scales Theses scales allow for easy identification of the specimen s position coordinates making it easy to return to a particular region of interest after scanning the slide 1 The horizontal coordinate can be read at position 1 on the specimen holder fig 18 2 The vertical coordinate can be read at the index line 2 Focusing Procedure 1 Rotate the coarse adjustment knob 1 clockwise so that the objective 3 is as close as possible to the specimen We recommend starting with 10X See figure 19 2 While observing the specimen through the eyepieces slowly rotate the coarse adjustment knob 1 counterclockwise to lower the stage 3 When coarse focusing of the specimen is obtained an image is detected rotate the fine
6. FF O gt gt gt Warning Label A warning indication label is attached to every part where special precaution is required while handling and using the microscope Always read the warnings Warning label Bottom of microscope Warning against high temperature in position frame bulb LED compartment Warning against risk of electric shock J Warning against damage in non N compliance with instructions manual If the warning label is stained or peeled off contact your LABOMED distributor Getting Ready 1 Amicroscope is a precision instrument with delicate glass components Please handle with care 2 Do not use the microscope where it is subjected to direct sunlight high temperature humidity dust and vibrations For the operating conditions see chapter 15 SPECIFICATIONS on Page 24 3 The microscope is ventilated by natural convection Be sure to leave enough space 10 cm or more around body while installing it 4 Arm handle is provided for carrying the microscope NTo prevent damage do not hold the microscope by the stage or observation tube Be sure to remove the specimen from the stage clip while transporting unitto avoid damage to the specimen slide Maintenance and Storage 1 Clean all glass components by wiping gently with cleaning cloth provided To remove fingerprints or oil smudges wipe with cleaning cloth slightly moi
7. LABOMED CxL User Manual Laboratory Microscopy To ensure proper use of this instrument as well as to avoid injury while operating instrument understanding this manual completely before use is highly recommended CONTENTS 1 INTRODUCTION The CxLis a laboratory microscope reflecting a modern design as well as the latest in optical and mechanical advancements Designed for professionals as well as students this microscope offers many features and functions for a diverse set of applications Extra clarity and contrast is provided through a 360 rotatable Binocular body inclined at 45 The pressure die cast stand consists of Ball bearing friction less sideways focusing to avoid any loss in motion The sturdy new stylish design provides comfort as well as stability The high powered objectives are spring loaded to prevent accidental damage to specimen slides The quadruple nosepiece has a comfortable ribbed grip for easy rotation that also safeguards the turret system against any damage All positions are par centered and par focalised ensuring the highest level of accuracy The ball bearing mechanical stage allows smooth travel over a 76 x 50mm area with spring loaded stage clamps for retaining the specimen atthe exact position desired A0 1mm vernier scale provides accurate location of specimen area The LED configuration is operational with an in built re chargeable battery and charging circuit The battery will be
8. Objective The designated immersion oil should be in contact with the top lens of the 100X immersion objective If not the specimen will appear distorted and dull It is recommended that LABOMED immersion oil is always used m Immersion Process TZ 1 Bring the specimen in focus using first the 10x then 40x objective LS J BEER a 2 Disengage the 40x cycling towards 100x and place a drop of immersion oil on the center point of the specimen 3 Rotate the revolving nosepiece to engage the immersion objective and rotate the fine adjustment knob to bring the specimen into focus Since air bubbles in the oil will affect the image quality make sure that the oil Is free of bubbles To remove bubbles rotate the revolving nosepiece slightly to agitate the oil 4 The condenser of this microscope manifests the full performance when oil is placed between the slide glass and the front lens of condenser If oil is not placed there the observed image may appear dark 5 After use remove oil from the objective front tens by wiping with lens tissue slightly moistened with petroleum 85 and isopropanol 15 mixture A Caution If immersion oil makes contact with your eyes rinse eyes out thoroughly with fresh water If immersion oil makes contact with skin wash affected areas with soap and water If prolonged discomfort is experienced consult your physician
9. brightness Observe Specimen _ DETAILED OBSERVATION PROCEDURE Turning the Lamp ON Fig 16 Flip the main switch to I ON as shown in figure 14 Rotating the light intensity adjustment knob fig 15 in the direction of the arrow increases brightness and rotating it in the opposite direction decreases brightness The figures around the knob indicate the intensity lever Place the specimen gently on the stage If the bow shaped lever 3 is returned with a strong force or the control knob 1 or the bow T shaped leveris released in the middle the slide glass may be broken P See figure 16 A Rotate the coarse adjustment knob 2 in anticlockwise direction to fully lower the stage Open the bow shaped lever 3 outward by pulling lever handle 1 place the specimen by sliding the specimen glass plate s on the stage from the front toward the rear After positioning your specimen slides return the bow shaped lever 3 gently by slowly releasing control knob 1 Rotating the upper co axial knob controlling the Y axis movement 4 moves the specimen in the vertical direction Rotating the lower knob which is the X axis movement control Knob 5 moves the specimen in the horizontal direction Do not adjust the specimen holder directly by hand for
10. cified immersion oilis notused Usethe immersion oil supplied by Labomed 5 Partof image is defocused The objective is not properly engaged Engage the objective into position inthe light path Until the nose turret clicks The specimen is not set properly on Setthe specimen correctly on the the stage stage and secure using the specimen holder 6 High magnification objective touches The specimen is upside down Setthe specimen correctly with the specimen just before coming into focus cover glass on the top 7 Coarse focus adjustment cannot The condenser is too low Raise the condenser lowerthe stage low enough 8 Fields of view of two eyes do not The interpupillary distance is not Adjust IPD match adjusted properly IPD compensation for the two eyes Adjustit correctly is notset Theleft and right eyepieces are of Replace one of them so that the left different magnification and right eyepieces are identical e 22 Trouble Cause Remedy 9 Objective hits the specimen when an objective is switched to a higher magnification objective The specimen is upside down Set the specimen correctly with the cover glass on the top The cover glass is too thick Use acover glass with thickness of 0 17mm The stage is raised too high Lower the stage The slide is slipped from the slide holder Re position the slide in the slide holder Slid
11. d set can be assembled by simply attaching filter and power cord Installing or Replacing the Bulb Before attaching the bulb remove the parts that may drop such as the filter and specimen from the microscope frame and place the microscope on its back so that the bottom plate is exposed 1 Pull the lock knob 1 on the bottom to open lamp housing door fig 11 2 Hold the halogen bulb 2 without taking it out of the polyethylene bag so as not to taint the bulb with fingerprints and push the bulb into the pin holes on the ocket 3 After attaching remove the polyethylene bag 3 With the lock knob pulled out close the lamp housing door then push the lock knob back to lock the cover Applicable bulb 6V20W Halogen bulb P N CX 013 Fig 11 Always use the designated bulb Using a bulb other than those specified by LABOMED may lead to a fire hazard Fingerprints or stains on the lamp bulb reduce its life If contamination occurs wipe bulb surface with a cloth slightly moistened with alcohol A Caution For Bulb ReplacementDuring Use or RightAfter Use The bulb lamp socket and areas near these will be extremely hot during and right after use Set the main switch to O OFF disconnect the power cord from the wall outlet and allow the bulb and lamp socket to cool before replacing the bulb with a new bulb of the designated type Cooling time may vary to users discretion Procedure for LED replace
12. diaphragm ring o Coarse and fine focus knob Intensity regulator CE X Y stage movement control knob e a 10 STANDARD COMPONENTS After removing microscope from packaging make sure that all of the following contents are present The differences in configurations are the number of objectives type of observation head type of illumination and purchased optional accessories m The objectives have been adjusted for a tight fit to prevent any damage during transportation To remove an objective rotate it counterclockwise while gripping it with a rubber sheet etc to avoid any slippage Focusable Eyepieces CO z Monocular Binocular Trinocular observation tube Allen screw Objectives Microscope Arm Stage o Condenser Daylight blue filter PowerCod wI SNS Qe Allen Wrench 3mm Allen Wrench 1 2mm 6V 20W Halogen bulb Spare fuses 11 CxL tmt OPTIONAL ACCESSORIES w System Diagram of Optional Accessories Photo adapter Photo eyepiece DSC adapter iVU 1500 1 1 1 J Video adapter L Monocular 1 LP 4x Papias LP 40x prase K amp g Eh A aED WOND e ts e e Abbe Condenser Phase Slider Polarizing Kit DF Slider Blue Fiter Green Filter l i 1 l Gee x Mirror Halogen Bulb LED M
13. e is of excessive thickness Use slide having thickness between 0 9 and 1 4mm 10 Bulb LED does not light Bulb LED is not mounted Attach a bulb LED Bulb LED is blown Replace the bulb LED The power cord is unplugged Plug it securely Fuse is blown Check and replace with live fuse Battery is low Charge battery 11 Bulb LED blows easily The specified bulb LED is not used Replace with a specified bulb LED SPECIFICATIONS CxL k Illumination Built in illumination system LED Halogen NM Focusing mechanism Stage height adjustment mechanism Fine adjustment scale 3 0um per graduation Fine adjustment stroke 0 2mm per turn Total stroke 12 7mm Co axial coarse and fine focusing on ball drive 3 Revolving nosepiece Quadruple positions fixed front oriented 4 Observation tube Monocular Binocular Trinocular Field number 18 18 18 Tube tilting angle 45 45 45 Interpupillary distance NA 54 74 54 74 adjustment range 5 Stage Size 135 x 124mm with mechanical stage Movement range 76 x 50mm Specimen holder Holds a single specimen 6 Condenser Type Abbe condenser daylight filter detachable N A 1 25 Aperture iris diaphragm Built in 7 Dimensions 255 0mm L x 227 0mm W x 375 23mm H 8 Electrical Battery 7 4V 1000mAH Charging time up to 5 hours with totally consum
14. ed battery Back up time up to 4 hours 9 Operating environment Indoor use Altitude Max 2000 meters Ambient temperature 5 to 40 C 41 to 104 F Maximum relative humidity 80 for temperature up to 31 C 88 F decreasing linearly through 70 at 34 C 93 F to 50 relative humidity at 40 C 104 F Supply voltage fluctuations Not to exceed 10 of the normal voltage Pollution degree 2 in accordance with IEC60664 Installation Overvoltage category II in accordance with IEC60664 24 www laboamerica com Our policy is one of continuous development Labo America Inc reserves the right to change design and specifications without prior notice Labo America Inc U S A 920 Auburn Court Telephone 510 445 1257 Fremont Fax 510 445 1317 CA 94538 sales laboamerica com LABOMED and CxL are registered trademarks of Labo America Inc With a policy of continuous development Labo America Inc reserves the right to change design and specifications without prior notice 2009 Labo America Inc 9135000 990A 12 2009 ISO 9001 2000 File No A9020 Printed in U S A
15. epeat the cleaning using a solvent Optical Cleaning Solution 85 petroleum ether and 15 isopropanol d Grease must always be removed using a solvent e Cleaning is achieved by using a spiral motion from the center to the rim Never wipe using zig zag movements as this will only spread the dirt With larger optical surfaces e g tube lenses the spiral motion starts initially at the rim before moving to the middle and is only then followed by a center to rim cleaning motion Normally several spiral wipes are recommended We recommend pure volatile petroleum ether or Optical Cleaning Solution as explained in point 3 above zig zag motion X spiral motion v Wipe using a spiral movement Do not use a zig zag motion 2 Cleaning of painted surfaces Avoid the use of any organic solvent e g thinner xylene ether alcohol etc for cleaning of painted surfaces of the instrument Painted surfaces can be cleaned with a very lightly moistened micro fiber cloth Loose dust and other dirt can be removed using a brush of soft hair used exclusively for this purpose Caution Do notuse aggressive organic solventsuch as acetone for cleaning painted surfaces and plastic parts of the microscope 3 Neverattemptto dismantle Never attempt to dismantle the instrument so as to avoid the possibility of impairing its operational efficiency and accuracy 4 Periodical checking To maintain the performance of the instrument we recommend custo
16. in blocking the air vents and cause overheating fire 4 Always use the power cord provided by LABOMED If the proper power cord is not used product safety performance cannot be warranted 5 When installing the microscope route the power cord away from the microscope frame Should the power cord come in contact with the microscopes base the power cord could short circuit 6 Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected If the equipment is not grounded LABOMED can not warrant the electrical safety performance of the equipment 7 Neverallow metallic objects to penetrate the air vents of the microscope frame as this could result in user injury and equipment damage 8 After operation of microscope be sure to disconnect power cord from connector on the microscope or from the wall power outlet Safety Symbols The following symbols are found on the microscope For optimal use it is recommended that users understand these symbols and always use the equipment as prescribed Symbol Explanation Indicates that the surface has a tendency to heat up and should not be touched unless system has completely cooled down Before use carefully read the instruction manual Improper use could result in injury to the user and or damage to the equipment Warning against risk of electric shock Indicates that the main switch is ON Indicates that the main switch is O
17. ment figure 12 1 Lay the microscope on aclean surface toward right so that the microscope base is exposed 2 Using a screw driver unscrew four screws 1 to open the bottom plate of the microscope 3 Remove two screws 2 provided on the lamp housing use screw driver 4 Replace existing LED mount with live LED mount 5 Reverse the steps 3 to 1 to complete the process Applicable LED replacement LED P N 9135000 901 Su a This filter modifies the color of observation light into a natural color daylight color 2 1 mann Il Fit the daylight filter 1 into the bottom of the condenser 2 until it _ L90 Jh clicks into place See figure 13 E i 1 T II 15 CxL I Z SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE Flip the main switch to ON Place the specimen on the stage Engage the 10X objective in the light path l Bring the specimen in focus Main switch Adjust the observation tube and eyepieces Adjust the interpupillary distance Adjust the dioptric setting Adjust the aperture iris diaphragm Engage the objective to be used in the light path and bring the specimen in focus Engage the required filters Adjust the
18. mers have their microscopes serviced periodically by a factory authorized dealer rep For details contact your nearest dealer or Labo America s main office in California CxL gt CxL Monocular Monocular viewing tube Revolving nosepiece Objectives Mechanical stage Coarse and fine focus knob X Y movement control knob 6 CxL Binocular Binocular viewing tube Revolving nosepiece Objectives Mechanical stage Coarse and fine focus knob Light regulator X Y movement control knob CxL CxL Trinocular C Port for camera adapter Focusable eyepieces Trinocular viewing tube e Revolving nosepiece Objectives z Mechanical stage Abbe condenser Light regulator s Coarse and fine focus knob SS X Y movement control knob OO 8 UNPACKING YOUR MICROSCOPE Mirror attachment if ordered Eyepieces Camera if ordered Observation tube Software CD if ordered Microscope Arm CxL SYSTEM DIAGRAM Focusable Eyepieces Wa e Observation tube clamping Revolving nosepiece Screw objectives Specimen holder s SS Mechanical stage Ze gt 0 Aperture iris
19. not specified by this manual the safety of the user may not be warranted In addition the equipment may also suffer damage Always use the equipment as outlined in this instruction manual Care amp Maintenance Your microscope has been engineered for a long and safe operational life with the least amount of maintenance required In general routine maintenance is limited to keeping the microscopes working parts lubricated and optics clean Always cover the microscope with the provided dust cover when notin use 1 Cleaning the lenses To clean the lens surfaces remove dust using a soft brush or gauze compressed air dust cans are ideal For removing finger marks or grease soft cotton cloth lens tissue or gauze lightly moistened with cleaning solution 85 petroleum ether and 15 isopropanol should be used For cleaning the objective optics use xylene Observe sufficient caution in handling xylene Cleaning procedure Place the objectives and or eyepieces on a dust free surface e g fresh aluminum foil All other optical components to be cleaned should be as accessible as possible a Blowallloose dust particles away with a dust blower b Remove all water soluble dirt with distilled water If this is unsuccessful repeat using a solution of diluted hand soap liquid Remove any remaining residue with a dry cotton swab c To remove oil use a solution of diluted hand soap liquid initially If this does not produce a satisfactory result r
20. ount Rechargeable Battery Installation and Operation of Optional Accessories Darkfield Slider The darkfield slider enables darkfield observation using an objective from 4X to 40X SeS oo Fig 4 12 Reflection Mirror The reflection mirror is designed to be used in out door observation settings wherein a power source is not readily available The mirror attachment makes it possible to use natural light to illuminate your specimen Fig 6 The microscope should be installed in any place that is not exposed to direct sunlight Direct sunlight reflection can cause eye injury or in some cases blindness The eyepieces should be oriented toward the rear of the microscope so that the reflection mirror receives unobstructed light To do this loosen the observation tube clamping knob and rotate the tube 180 A Fit the reflection mirror in the window lens of the microscope frame by aligning the mounting tab Remove the daylight filter because it renders the image blue under natural light conditions The reflection mirror fig 5 should be pointed toward anywhere with stray light While observing the image through the eyepieces adjust the orientation of the reflection mirror to capture stray light planar reflection minor is used normally However if the image brightness is uneven or the outside view is visible in the image
21. stened with a mixture of petroleum 85 and isopropanol 15 Since solvents such as petroleum and isopropanol are highly flammable they mustbe handled carefully Be sure to keep these chemicals away from open flames or potential sources of electrical sparks for example electrical equipmentthat is being switched ON or OFF Also remember to always use these chemicals only in a well ventilated room 2 Donotattemptto use organic solvents to clean the microscope components other than the glass components To clean non glass components use a lint free soft cloth slightly moistened with a diluted neutral detergent 3 Do not disassemble any part of the microscope as this could result in malfunction or mitigated performance 4 When not using the microscope ensure that the frame is fully cooled and store the unit in a dry locker or cover with a dust cover provided 5 To clean the condenser fully loosen the securing knob lower and remove the condenser by lowering condenser bracket wipe the front lens of the condenser with optical cleaning solution mixture suggested above and lens tissue The condenser can be re attached by replacing the condenser in its seat tightening securing screws and raising condenser bracket to desired position Fig 3 6 Besuretoobserve yourlocal rules regulations for product disposal 3 CxL Caution If the microscope is used in a manner
22. use a concave reflection mirror The standard 10X eyepieces are clamped using screws 1 Using allen wrench 1 2mm loosen the clamping screw 1 from the ocular tube and remove it Insert the optional eyepieces into the ocular sleeve and tighten the clamping screw 1 CxL INITIAL SET UP Fig 7 Objectives are factory set Objectives are par centered and par focalised during assembly phase All objectives have been secured for a tight fit to prevent them from coming loose during transit To remove an objective rotate it counterclockwise while holding it with a rubber sheet etc to avoid any slippage Install the observation head using following procedure 1 Using allen wrench 3mm loosen the Head Locking Screw 1 and remove the dust cover cap provided in dovetail cavity as well as on observation head dovetail 2 Mount the Observation Head by engaging the dovetail provided at the bottom of the head into the dovetail cavity provided in the microscopes arm 3 Tighten the Head Locking Screw 1 after positioning the Observation Head as desired Insert the eyepieces into the ocular tube of Observation Head using following procedure 1 Remove the protective caps from the observation tube 2 Insert 10x eyepieces into the ocular sleeve and tighten the clamping screw 1 using allen wrench 1 2mm See figure 10 Each standar
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