Home

CY-7051

1. 4 5 6 7 SN 38 treatment time hr Roa CY 7051 11 Version 140318 o c ww Phospho p53 Ser392 ELISA Kit gt O e ycLex User s Manual AS For Research Use Only Not for use in diagnostic procedures m __ lt lt lt 3 4 5 6 Ts 8 9 10 11 12 13 14 Hollstein M Sidransky D Vogelstein B and Harris C C 1991 Science 253 Greenblatt M S Bennett W P Hollstein M and Harris C C 1994 Cancer and Bradley A 1992 Nature 356 215 221 Harvey M McArthur M J Montgomery C A Jr Bradley A and DonehoweQ y A 1993 FASEB J 7 938 943 54 4855 4878 Chen H E Chang S Trub T and Neel B G 1996 Mol Cell Biol 16 3685 697 Honda R Tanaka H and Yasuda H 1997 FEBS Lett 420 25 27 Yon Kubbutat M H Jones S N and Vousden K H 1997 Nature 387 29 Haupt Y Maya R Kazaz A and Oren M 1997 Nature 387 oe Ni McCoy M A Gesell J J Senior M M and Wyss D F 2003 PRS atl Acad Sci U S A 100 1645 1648 Barak Y Juven T Haffner R and Oren M 1993 EMBO J 1246 1 468 Appella E and Anderson C W 2001 Eur J Biochem 268 4 2772 Wahl G M and Carr A M 2001 Nat Cell Biol 3 E277 Buschmann T Potapova O Bar Shira A Ivanov V N Fuchs S Y Henderson S Fried V A Minamoto T Alarcon Vargas D Pincus M R
2. Gaard A Holbrook N J Shiloh Y and Ronai Z 2001 Mol Cell Biol 21 2743 2754 Dumaz N and Meek D W 1999 EMBO J 18 70097010 Jabbur J R Huang P and Zhang W 2000 Onc egne 19 6203 6208 Oda K Arakawa H Tanaka T Matsuda K nikawa C Mori T Nishimori H Tamai K Tokino T Nakamura Y and Taya Y 2000 102 849 862 Kapoor M and Lozano G 1998 Proc N cad Sci USA 95 2834 2837 Lu H Taya Y Ikeda M and Levine A 98 Proc Natl Acad Sci USA 95 6399 6402 Hupp T R Meek D W Midgley C A Lane D P 1992 Cell 71 875 886 Sakaguchi K Sakamoto H Lewis Anderson C W Erickson J W Appella E and Xie D 1997 Biochemistry 36 10117 101 References A RA Levine A J 1997 Cell 88 323 331 gt 2 Donehower L A Harvey M Slagle B L McArthur M J Montgomery C A p J S Roa CY 7051 12 Version 140318 o c S Phospho p53 Ser392 ELISA Kit 9 ycLex User s Manual 3 For Research Use Only Not for use in diagnostic procedures 4 Related Products 3 CycLex Total p53 ELISA Kit Cat CY 7049 we CycLex Phospho p53 Ser46 ELISA Kit Cat CY 7050 RY CycLex Phospho p53 Ser392 ELISA Kit Cat CY 7051 Anti Phospho p53 Ser46 TK 4D4 monoclonal antibody Cat CY M1022 g wW gt eo PRODUCED BY amp Y xs CycLex Co Ltd 1063 103 Terasawaoka xO Ina Nagano 396 0002 S Japa
3. OR IMPLIED rA Roa CY 7051 7 Version 140318 o c amp the Stop Solution Roa CY 7051 8 Version 140318 C 2 Ss Phospho p53 Ser392 ELISA Kit Pa P ycLex User s Manual For Research Use Only Not for use in diagnostic procedures ELISA N AN Remove the appropriate number of microtiter wells from the foil pouch and place them in afh e well holder Return any unused wells to the foil pouch refold seal with tape and store at 4 C N Dilute the lysate 1 10 with Dilution Buffer e g 30 uL sample 270 uL Dilution Bulg Lysates prepared in Cell Extraction Buffer must be diluted 1 10 or greater in Dilu Sauter While a Nn oOo 9 10 11 12 13 14 15 o AO 1 10 lysate dilution has been found to be satisfactory higher dilutions such au or 1 40 may be optimal The dilution chosen should be optimized for each experimental YSTER Pipette 100 uL of Standard Solutions Std1 Std7 Blank and diluted rog in duplicates into the appropriate wells Incubate the plate at room temperature ca 25 C for 1 hour shal at ca 300 rpm on an orbital microplate shaker A Wash 4 times by filling each well with Wash Buffer 350 using a squirt bottle multi channel pipette manifold dispenser or microplate washer Add 100 uL of Primary Antibody Solution into each wg Incubate the plate at room temperature ca 25 C for 1 hour shaking a
4. for use in diagnostic or therapeutic procedures Storage c C e Upon receipt st omponents at 4 C e Don t expose gens to excessive light RS Q Ki Reo Qe Phospho p53 Ser392 ELISA Kit P 4 r ycLex User s Manual For Research Use Only Not for use in diagnostic procedures 4 A Q O Q Introduction 3 p53 is a short lived non abundant protein that regulates the response of cells to DNA dam n part through transcriptional activation of genes involved in cell cycle control DNA repair and apQptosis 1 p53 is a tumor suppressor protein consequently mice in which the p53 gene has been disrapied develop tumors with high frequency 2 3 and deletions or mutations in the p53 gene are prevalegfyin a majority of human cancers 4 5 The protein level and activity of p53 are regulated and this i omplished by MDM2 murine double minute 2 6 10 The MDM2 gene is induced by p53 piipu prevents apoptosis by inhibiting p53 activity and promoting its degradation 6 9 11 In response to DNA damage the p53 protein is phosphorylated on each of the seven serines and one threonine the in the first 50 amino acids of its N terminal transactivation domn as well as at several sites in its C terminal tetramerization regulatory domain 12 13 As a raQPrion factor p53 induces or represses several genes that regulate cell cycle arrest DNA repair or apoptosis including p21 WAFI MDM2 GADD45 p53R2 and p53AIP1 Recent stud
5. Ss Phospho p53 Ser392 ELISA Kit Pa ycLex User s Manual For Research Use Only Not for use in diagnostic procedures gt ELISA Kit for Measuring Phosphorylated Human p53 Ser392 l CycLex Phospho p53 Ser392 ELISA K Cat CY 7051 O Intended ISG ocaracarsaanteanadniotaiennecissamkectond 1 Meg SOOO Se T 1 oO TCO G HOM pcp 5385s ddoweacasomneuarniuene 2 aS Principle Of the Assay 2 3 XN Materials Provided cccscceceesseceeereeeeees 4 Yon Materials Required but not Provided 4 gt Precautions and Recommendations 5 N Detailed Proto jaicassescessvetavesssncsessuaniooensees 6 9 KS AL CRL ALI ONS sich sssini risinn 9 re Measurement RAN isis csscustsaneteuceetstarntiaamts 9 Q Troubleshooting eeeseeeeeeeeeeeerrererrrerrerreeee 9 10 Reagent Stability 10 gt Assay Characteristics 10 Example of Test RESUS sjsscssstansstohssaceencsss PY Refer nt S rissen 6 Related Proqucts issssesenesevsvessasecsastenaveness 3 Intended Use sO The CycLex Research Product Cyelg Phospho p53 Ser392 ELISA Kit is designed to detect and quantify the level of human p53 ro that is phosphorylated at serine 392 Since the amino acid sequence surrounding serine 392 is conserved in mouse and rat p53 this ELISA kit can not be used for mouse and rat cells This assagfjs intended for the detection of phosphorylated human p53 at serine 392 from cell lysate X This assay kit is for ss only and not
6. Wash cells twice with cold PBS KG 3 Remove and discard the supernatant and collect t pellet At this point the cell pellet can be frozen at b 70 C and lyse at a later date 4 Lyse the cell pellet in 0 5 mL of Cell Exon Buffer for 30 minutes on ice with vortexing at 10 minute intervals Py To get a rough idea you co djust the cell concentration to around 2 x 10 cells mL Resulting protein concentratiOm of the cell lysate should be 2 4 mg mL using this Cell Extraction Buffer 74 The volume of Cell raction Buffer depends on the cell number in cell pellet and Phosphorylation leve 53 For example 1 2 x 10 MCF 7 cells in 10 cm dish can be extracted in 0 5 mL Cell Extraction Buffer Under these conditions the protein concentration should be 2 4 m and use of 5 10 uL of the clarified cell extract diluted to a volume of 100 ul well in Diluti uffer is sufficient for the detection of phospho p53 Ser392 5 Transfer the lysat Wp microcentrifuge tubes and centrifuge at 13 000 rpm for 10 minutes at 4 C 6 Aliquot the cl ysate to clean microfuge tubes These samples are ready for assay The lysates can be stored at w 70 C Avoid multiple freeze thaw cycles NOTE tR anove PROCEDURES ARE INTENDED ONLY AS A GUIDELINE THE TIMAL EXPERIMENTAL CONDITIONS WILL VARY DEPENDING ON THE PARAMETERS BEING INVESTIGATED AND MUST BE DETERMINED BY THE INDIVIDUAL USER NO WARRANTY OR GUARANTEE OF PERFORMANCE USING THESE PROCEDURES IS MADE
7. absorbance of blank A blank 3 SD blank is better than 1 418 wnits ml of sample Dilution Buffer is pipetted into fe wells Eighty assays were evalu and the minimum detectable dose MDD of Phospho p53 Ser392 ranged from 0 723 1 624 s mL The mean MDD was 1 413 units mL The MDD was determined by adding three standard d vjations to the mean optical density value of twenty zero standard replicates and calculating the corr ding concentration 2 Specificity The antibodies in the CycLex Phospho p53 Ser392 ELISA Kit are highly specific of human Phospho p53 seg with no detectable cross reactivity to mouse and rat Phospho p53 3 Linearity To asse linearity of the assay samples containing and or spiked with high concentrations of Phosp Ser392 were serially diluted with the Dilution Buffer to produce samples with values within W dynamic range of the assay S amp Phospho p53 Ser392 ELISA Kit gt Pi Oo ycLex User s Manual lt For Research Use Only Not for use in diagnostic procedures amp Example of Test Results 3 Fig 1 Typical standard of phospho p53 Ser392 N Standard curve K 0 20 40 60 80 100 Phospho p53 S392 U ml w Fig 2 Phospho p53 Ser392 ELISA using breast c lt a line MCF 7 that expresses wild type p53 which have been treated with 10 uM SN 38 ndicated time ue Phospho p53 392 ELISA MCF 7 treated with 10 uM SN 38 for indicated time 35 F A450 0 1 2 3
8. and read the corresponding Phospho p53 Ser392 concentration If the samples hav en diluted the concentration read from the standard curve must be multiplied by the dilution factogy A The dose response curve of this assay fits beste a sigmoidal 5 parameter logistic equation The results of unknown samples can be calcul with any computer program having a 5 parameter logistic function It is important to make an A rropriate mathematical adjustment to accommodate for the dilution factor B Most microtiter plate readers performtomatic calculations of analyte concentration The calibration curve is constructed by plotting h absorbance Y of calibrators versus log of the known concentration X of calibrators ng the four parameter function Alternatively the logit log function can be used to lineariz calibration curve i e logit of absorbance Y is plotted versus log of the known concentration RC calibrators Measurement R The measurement raya 1 56 units mL to 100 units mL Any sample reading higher than the highest standard should be S d with Dilution Buffer in higher dilution and re assayed Dilution factors need to be taken into consi ration in calculating the phospho p53 Ser392 concentration Ra amp gt o G oe Roa CY 7051 9 Version 140318 o c Roa CY 7051 10 Version 140318 ge A Ss Phospho p53 Ser392 ELISA Kit e cA ycLex User s Manual For Research Use Only Not for use in diagnos
9. b Of strips required for the particular determination Since experimental conditions may vary an Sr of the Phospho p53 Standard within the kit should be included in each assay as a calibrator Digpysable pipette tips and reagent troughs should be used for all liquid transfers to avoid oropan Re of reagents or samples Y Preparation of Working Solutions wW All reagents need to be brought to room temperature prior to the assay reagents are supplied ready to use with the exception of 10X Wash Buffer Cell Extraction uffer and Phospho p53 Ser392 Standard rw 1 Prepare a working solution of Wash Buffer by adding 100 mL of yx Wash Buffer to 900 mL of deionized distilled water ddH20 Mix well y 2 Prepare a working solution of Cell Extraction Buffer by addi 50 uL of Protease inhibitor cocktail Sigma Cat P 2714 to 5 mL of Cell Extraction Buffer Nix well 3 Reconstitute Phospho p53 Ser392 Standard with 1 L of ddH20 The concentration of the phospho p53 Ser392 in vial should be 200 units mL which is referred as a Master Standard of phospho p53 Ser392 A s Prepare Standard Solutions as follows Use the Master Standard to produce a HRon series below Mix each tube thoroughly before the next transfer The 100 units mL stadard Std 1 serves as the highest standard The Dilution Buffer serves as the zero standard Volume of a Dilution Buffer Concentration Std 1 300 uL of a 300 uL 100
10. ecision pipettors with disposable tips e Precision repeating pip ttor e Microcentrifuge and tubes for sample preparation e Vortex mixer e Microplate wash Saal Manual washing is possible but not preferable e Plate reader capabl of measuring absorbance in 96 well plates at dual wavelengths of 450 nm 540 nm Dual wav acts of 450 550 or 450 595 nm can also be used The plate can also be read at a single wave of 450 nm which will give a somewhat higher reading e Software p ge facilitating data generation and analysis optional e 500 or 1000 graduated cylinder Reage Servoirs Deiofiized water of the highest quality e Disggsable paper towels oe Roa CY 7051 4 Version 140318 o AO S Phospho p53 Ser392 ELISA Kit 9 ycLex User s Manual 3 For Research Use Only Not for use in diagnostic procedures 4 Precautions and Recommendations 3 e Allow all the components to come to room temperature before use we All microplate strips that are not immediately required should be returned to the zip lock Na which must be carefully resealed to avoid moisture absorption g e Do not use kit components beyond the indicated kit expiration date Yon e Use only the microtiter wells provided with the kit e Rinse all detergent residue from glassware e Use deionized water of the highest quality RU S Y A N e Do not mix reagents from different kits amp The buffers and reage
11. for use in diagnostic procedures 4 gt Note 1 Complete removal of liquid at each step is essential to good performance After Ajast wash remove any remaining Wash Buffer by aspirating or decanting Invert these and blot it against clean paper towels Note 2 Reliable standard curves are obtained when either O D values do not exceed units for the blank zero concentration or 2 5 units for the highest standard N ta The plate should be monitored at 5 minute intervals for approximately 30 minutes Note 3 If the microplate reader is not capable of reading absorbance greater tha absorbance of the highest standard perform a second reading at 405 nm A standard curve constructed using the values measured at 405 nm is used to shoul ae ospho p53 Ser392 concentration of off scale samples The readings at 405 nm shoul replace the on scale readings at 450 nm Calculations Average the duplicate readings for each standard control and saple and subtract the average zero standard optical density Plot the optical density for the stand versus the concentration of the standards and draw the best curve The data can be linearize using log log paper and regression analysis may be applied to the log transformation To determige the Phospho p53 Ser392 concentration of each sample first find the absorbance value on the y axis extend a horizontal line to the standard curve At the point of intersection extend a vertical Ra the x axis
12. ies suggest thi ecific p53 phosphorylation events are important for the activation or repression of specific promot 14 17 Phosphorylation of human p53 Ser 392 C terminal regulatory doin occurs following UV but not ganmma irradiation 18 19 and results in enhancement of sequ specific binding activity in vitro 20 possibly by promoting stable tetramer formation 21 Principle of the Assay 2 The CycLex Research CycLex Phospho p53 Ser392 BLISA Kit is a solid phase sandwich ELISA An antibody specific for human p53 has been coate to the wells of the microtiter strips provided Samples including a standard containing phospho d form of p53 at Serine 392 control specimens and unknowns are pipetted into these wells Duri e first incubation p53 protein binds to the capture antibody on the well After washing mouse oclonal antibody specific for phosphorylated p53 Ser392 as a detection antibody is added to thg wells During the second incubation this antibody serves as a detector by binding to the pe een osphorylated p53 protein at Ser392 captured during the first incubation After removal of exce etection antibody followed by binding with horseradish peroxidase conjugated anti mouse LAS hich then catalyzes the conversion of the chromogenic substrate tetra methylbenzidine TMB from a colorless solution to a blue solution or yellow after the addition of stopping reagent The 9 or is quantitated by spectrophotometry and reflects the relative amou
13. n e2 Fax 81 265 76 7618 KX e mail info cyclex co jp amp URL http www cyclex co j Q CycLex CircuLex products are Syupplied for research use only CycLex CircuLex products and components thereof may ee modified for resale or used to manufacture commercial products without prior wr approval from CycLex Co Ltd To inquire about licensing for such commercial use pleage ntact us via email RX Q O A Roa CY 7051 13 Version 140318 o AO
14. nt of acetylated form of p53 serine 392 present in the original specimen S o oe Roa CY 7051 2 Version 140318 e ee A gt Phospho p53 Ser392 ELISA Kit re ycLex User s Manual For Research Use Only Not for use in diagnostic procedures 4 Summary of Procedure l Culture cells in culture flask or dish at 50 70 confluency SS Incubate O N at 37 C in CO incubator S Add appropriate amount of the drug for induction of p53 Ser392 oo 4 Incubate appropriate time at 37 C in CO2 incubator g Harvest the cells by scraping and centrifugation F Make cell lysate by adding extraction buffer and centrifugati S i gt Add 100 uL of diluted cell lysate to the wells 4 Incubate for 1 hour at room temp N Wash the wells o Add 100 uL of Primary Antibody Solution quFPospho ps 3 Ser392 monoclonal antibody 4 Incubate for 1hour at roq temp Wash the wells SS t O Add 100 uL of Secondary Antiledy Solution HRP conjugated anti mouse IgG antibody 4 Incubate fo ur at room temp Wash the wells amp amp Add 100 uL of Subte Reagent Lor Add 100 Laf op Solution Meanie psorbance at 450 nm F amp a 2 Roa CY 7051 3 Version 140318 e c A Ss Phospho p53 Ser392 ELISA Kit e cA ycLex User s Manual For Research Use Only Not for use in diagnostic procedures 4 Materials Provided 3 All samples and standards should be assayed in duplicate The following components a
15. nts in this kit may contain preservatives ier chemicals Care should be taken to avoid direct contact with these reagents Do not mouth pipette or ingest any of the reagents RZ e Do not smoke eat or drink when performing the say or in areas where samples or reagents are handled A e Dispose of tetra methylbenzidine TMB contei g solutions in compliance with local regulations e Avoid contact with the acidic Stop Soluig Substrate Solution which contains hydrogen peroxide e Wear gloves and eye protection whet Fandting immunodiagnostic materials and samples of human origin and these reagents In case ontact with the Stop Solution and the Substrate Solution wash skin thoroughly with water and se gmedical attention when necessary Biological samples may Pacer ssaiel with infectious agents Do not ingest expose to open wounds or breathe aerosdls Wear protective gloves and dispose of biological samples properly handli p Solution O nw a Qe amp eo Roa CY 7051 5 Version 140318 e CAUTION handia id is a strong acid Wear disposable gloves and eye protection when o AO Phospho p53 Ser392 ELISA Kit P 4 r ycLex User s Manual For Research Use Only Not for use in diagnostic procedures 4 A Q O Q Detailed Protocol 3 The CycLex Research Product CycLex Phospho p53 Ser392 ELISA Kit is provided with vable strips of wells so the assay can be carried out on separate occasions using only the num
16. re uta and are sufficient for the one 96 well microplate kit RY Microplate One microplate supplied ready to use with 96 wells 12 strips of 8 wells foil zip lock bag with a desiccant pack Wells are coated with anti human p53 antibody as a epr ws ody 10X Wash Buffer One 100 mL bottle of 10X buffer containing 2 Tween 20 2 K Cell Extraction Buffer One bottle containing 20 mL of 1X buffer For 1R term storage over 3 months store at 20 C a Dilution Buffer One bottle containing 50 mL each of 1X buffer use for ple dilution Ready to use Phospho p53 Ser392 Standard One vial containing 240 units amp yophilized phosphorylated p53 Ser392 Primary Antibody Solution Anti Phospho p53 Ser392 ph iors Antibody FPS392 One vial containing 12 mL of Anti Phospho p53 Ser392 monoclonal ay ody Ready to use Secondary Antibody Solution HRP conjugated AnthSlouse IgG One vial containing 12 mL of HRP horseradish peroxidase conjugated anti mouse IGG antibody Ready to use Substrate Reagent One bottle containing 20 mL the chromogenic substrate tetra methylbenzidine TMB Ready to use x Stop Solution One bottle supplied ready L containing 20 mL of 1 N H2SO Materials Required but n rovided e Protease inhibitor cocktail ex S igma Cat P 2714 reconstituted according to manufacturer s guideline Add 250 uL per 5 mfQ ell Extraction Buffer e Orbital microplate shaker e Pipettors 2 20 uL 20 20 and 200 1000 uL pr
17. t ca 300 rpm on an orbital microplate shaker Wash 4 times by filling each well with Wasepuffer 350 uL using a squirt bottle multi channel pipette manifold dispenser or microplate wa hgr Add 100 uL of Secondary Antibody Sion into each well Incubate the plate at room tem erature ca 25 C for 1 hour shaking at ca 300 rpm on an orbital microplate shaker Be Wash 4 times by filling ae with Wash Buffer 350 uL using a squirt bottle multi channel pipette manifold dispens icroplate washer Add 100 uL of Substrate Reagent Avoid exposing the microtiter plate to direct sunlight Covering the plate with e g um foil is recommended Return Substrate A to 4 C immediately after the necessary volume i femoved Incubate the plate at room temperature ca 25 C for 10 20 minutes shaking at ca 300 rpm on an orbital micr e shaker The incubation time may be extended up to 30 minutes if the reaction temperatu elow than 20 C Add Q of Stop Solution to each well in the same order as the previously added Substrate Re M asure absorbance in each well using a spectrophotometric microplate reader at dual wavelengths Qf 450 540 nm Dual wavelengths of 450 550 or 450 595 nm can also be used Read the microplate t 450 nm if only a single wavelength can be used Wells must be read within 30 minutes of adding A S Phospho p53 Ser392 ELISA Kit 9 A ycLex User s Manual 3 For Research Use Only Not
18. tic procedures 4 Troubleshooting 3 1 The Human Phospho p53 Ser392 Standard should be run in duplicate using the protocol Koea in the Detailed Protocol Incubation times or temperatures significantly different from thos amp sspecified may give erroneous results 2 Poor duplicates accompanied by elevated values for wells containing no sample i te insufficient washing If all instructions in the Detailed Protocol were followed accurately s esults indicate a need for washer maintenance Q 3 Overall low signal may indicate that desiccation of the plate has occurred been the final wash and addition of Substrate Reagent Do not allow the plate to dry out Add S te Reagent immediately after wash Reagent Stability S All of the reagents included in the CycLex Research Product ex Phospho p53 Ser392 ELISA Kit have been tested for stability Reagents should not be used amp gYond the stated expiration date Upon receipt kit reagents should be stored at 4 C except the rekonstituted Human Phospho p53 Ser392 Standard must be stored at below 70 C Coated assay pl should be stored in the original foil bag sealed by the zip lock and containing a desiccant pack R For research use only not for use in diagnostic or peutic procedures Assay Characteristics Q 1 Sensitivity 4 The limit of detection defined as suck oncentration of Phospho p53 Ser392 giving absorbance higher than mean absorbance of blank us three standard deviations of the
19. units mL Std 2 300 uL of St 100 units mL 300 uL 50 units mL Std 3 300 uL ofk 2 50 units mL 300 uL 25 units mL Std 4 300 uL td 3 25 units mL 300 uL 12 5 units mL Std 5 300 b Std 4 12 5 units mL 300 uL 6 25 units mL Std 6 30 f Std 5 6 25 units mL 300 uL 3 13 units mL Std 7 a of Std 6 3 13 units mL 300 uL 1 56 units mL Blank 300 uL O units mL u bef ispensing Unused portions of Standards should be aliquoted and stored at below 70 C i ately Avoid multiple freeze and thaw cycles Q S 72 Note Do amp a Repeating pipette Change tips for every dilution Wet tip with Dilution Buffer Roa CY 7051 6 Version 140318 o AO Ss Phospho p53 Ser392 ELISA Kit Pa ycLex User s Manual For Research Use Only Not for use in diagnostic procedures s gt Assay Procedure l A Treatment of Cells with compounds 1 Plate adherent cells or non adherent cells in culture flasks at 50 70 confluency KS 2 Incubate the culture flasks at 37 C over night in CO2 incubator N 3 Add appropriate amount of test compounds to each flask 2 4 Incubate the culture flasks at 37 C for appropriate time B Cell Extraction y D gt Note This protocol has been successfully applied to several cell li sers should optimize the cell extraction procedure for their own applications Y 1 Collect cells in PBS by centrifugation non adherent cells of amp scraping from culture flasks adherent cells 2

CY-7051

Contents

Download Pdf Manuals

Related Search

Related Contents