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Typhoon™ FLA 7000 - GE Healthcare Life Sciences

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1. e Protect the phosphor stage with the protective cover when removing an exposed storage phosphor screen from the cassette and placing it on the Phosphor stage Set the exposure time Set the exposure time so that the exposure finishes right before the reading starts Note The exposure time of the storage phosphor screen is approximately one twentieth of the time required for X ray film Take this into consideration when setting the initial exposure time TIP For increased image quality minimize the time between exposing the storage phosphor screen and reading it with the Typhoon FLA 7000 78 Typhoon FLA 7000 User Manual 28 9607 62 AB 11 About storage phosphor screens 11 5 Exposing the storage phosphor screen 11 5 Exposing the storage phosphor screen Step Action il Erase the storage phosphor screen completely 2 Wrap the radio isotope sample with plastic film Make sure not to wrinkle the wrapping film Note When using a tritium storage phosphor screen to detect tritium place the sample directly on the storage phosphor screen A tritium storage phosphor screen can be used only once 3 Open the top cover of the cassette Unlock the cassette by sliding the buttons on both sides up Note Keep the radio imaging sample away from the edge of the storage phos phor screen Otherwise the recorded data may become corrupted Typhoon FLA 7000 User Manual 28 9607 62 AB 79 11 About storage phosphor screens 11 5 Exposi
2. Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood Cautions CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood Typhoon FLA 7000 User Manual 28 9607 62 AB 5 1 Introduction 1 1 Important user information Notices NOTICE gt NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips Note A Note is used to indicate information that is important for trouble free and optimal use of the product TIP A tip contains useful information that can improve or optimize your procedures Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch 1 2 Regulatory information This section lists the directives and standards that are fulfilled by the Tyohoon FLA 7000 system Manufacturing information Requirements Content Name and address of manufacturer GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Swe
3. ok n 5 hnhoon LA 7000 Dl use cin Dirina Me zone DosM g i liesalhpe Se pla ili 13 14 Click the OK button in the Install from disk dialog 15 Click the Next button in the Add hardware wizard 16 Click the Next button again 17 Click the Continue Anyway button in the Hardware Installation dialog Hordvare Installation The soltuare ja are ins aling lor thir haidware FIMA AS 1 S3 Tevi te M rieynent Drives has rot passac v indowe Lego tasting o verif t compatibiiy wth Wirdows tI Lel me ww this testira is nacranl I Continuing your installation of this sottware may impair ar destahilize the ranect aperation nf grau system either immediately or in the tuturc Microzolt stionaly recommend that you stop thit installation now and contact the hardware vendo for software that has passed Windows Logo testing Centnuea veav STOP Irstalstix 18 Click the Finish button to complete the driver installation 38 Typhoon FLA 7000 User Manual 28 9607 62 AB 5 Installing Typhoon FLA 7000 Control Software 5 2 Install Typhoon FLA 7000 Control Software under Windows XP Install the USB function driver Windows XP Step Action 1 Connect the computer and the Typhoon FLA 7000 with a USB cable and turn ON the power switch of Typhoon FLA 7000 The scanner is automatically detected by the com puter In the Found New Hardware Wizard dialog choose No not this time Welcome la The
4. 3 Comment enter an optional comment The comment is embedded in the file where the image is saved and can be viewed with the analyzing software 4 Method set the method to use in the scan Up to 4 scans can be performed in a row all with individual methods 5 PMT set the voltage of the photo multiplier tube The higher the value the higher the sensitivity Click to increase the number of scans Click to decrease the number of scans Typhoon FLA 7000 User Manual 28 9607 62 AB 11 1 Introduction 1 4 Typhoon FLA 7000 Control Software Part Description 6 Set the scanning area 1 Select a stage in the drop down menu 2 Drag the red square to the desired position of the scanning area 3 Drag the sides of the red square as needed to adjust the size of the scanning area Pixel Size set the pixel size Choose a small pixel size for high quality images Note that a small pixel size setting increases the reading time and the size of the image file Latitude Specify the dynamic range e L5 provides a larger detectable range than L4 e L4provides a finer density gradation if the signals of the sample areas are in the correct range Mode Specify the mode for the reading area borders e Select Grid to limit the borders to the grid lines e Select Free for an arbitrary area e Select All to use the entire Fluor stage as the reading area 10 Save Condition click this button to
5. s 0580 EtBr 532nm 0580 FAM 473nm BLV520 FITC 473nm rs520 Phosphor EBssonm PI Pro Q Diamond 3532nm 0580 pi Delete Edit Add Cancel OK 2 Select the desired method to edit 3 Click the Edit button 4 Enter the desired changes 5 Click the OK button 6 Finalize the changes by clicking the OK button in the Method Settings dialog box Note Default methods cannot be edited Names of default methods are surrounded by square brackets 7 3 Deleting a method Note Default methods cannot be deleted Names of default methods are surrounded by square brackets Typhoon FLA 7000 User Manual 28 9607 62 AB 49 7 Registering editing and deleting methods 7 3 Deleting a method Action Click the Method button in the main window The Method Settings dialog box appears LS Method Settings Method List Method Name 7 Laser __ FiterName JE 10580 3532nm 0580 3535nm BIRG70 DDAO 635nm R670 Deep Purple Gj532nm s 0580 EtBr 532nm 0580 FAM 3473nm rvs20 FITC 473nm r520 Phosphor s5onm BPI Pro Q Diamond EB532nm rosso E Delete Edit Add Cancel OK Select the desired method to delete Click the Delete button Click the Yes button A confirmation dialog appears In the screenshots below the method name given is no name dd Are you sure you want to delete this method no name Finalize the c
6. Deep Purple Gj532nm s 0580 EtBr 532nm 10580 FAM 3473nm rv52o FITC E473nm rs520 Phosphor EBsonm Ss BLIP Pro Q Diamond Gj532nm s 0580 si Delete Edit Add Cancel OK Typhoon FLA 7000 User Manual 28 9607 62 AB 47 48 7 Registering editing and deleting methods 7 1 Registering a new method Step Action 2 Click the Add button The following dialog box appears Method a U Name TEE Laser ED s32nm Filter BB rvszol ox Cancel Select a laser in the Laser drop down box You can select lasers that are not currently loaded Select a filter in the Filter drop down box You can select filters that are not currently loaded Type a name in the Name field Click the OK button The list in the Method Settings window now contains the new method with the selected laser and filter combination Note Methods containing lasers or filters that are not currently loaded cannot be selected in the Reader Condition screen Typhoon FLA 7000 User Manual 28 9607 62 AB 7 Registering editing and deleting methods 7 2 Editing a method 7 2 Editing a method Step Action 1 Click the Method button in the main window The Method Settings dialog box appears Method Settings Method List Method Name Laser Fitername JF 310580 Cy3 532nm s 0580 Cy5 635nm R670 DDAO 3635nm BIRG70 Deep Purple Gj532nm
7. 11 2 Compatible storage phosphor SCreens pes aeasaeencatasinai cacao eat mean age a nu a it 76 TLs Handling eC ANTICS aan aaa e MA pa da 76 11 4 Preparing the storage phosphor Screen saia US e ia 78 11 5 Exposing the storage phosphor Screen icasachcescssscsesasevoccescinabanesepesvoscnesossinneoyeerbesshdoversuodebapseostassnshacstbecchehconielianis 79 lo IVES FUNC RER E E EE RS A RR E A E RP at 81 Troubleshoot Ng rrer ERE PE IAR RA PART e eisses eA RA A RA NES IE A RIP RAR 83 D fault sample detection MELO ssa sia asa dia pe Rae iz a iai bi eee i op et a 87 STN Lea vile IN Soccer aces eens RC ves a N A AIR SE E 89 Typhoon FLA 7000 User Manual 28 9607 62 AB 1 Introduction 1 Introduction In this chapter This chapter contains important user information and a general description of Typhoon FLA 7000 and its intended use 1 1 Important user information Read this before using the Typhoon FLA 7000 All users must read this entire manual to fully understand the safe use of Typhoon FLA 7000 Intended use Typhoon FLA 7000 is a fast laser scanner optimized for quantitative phosphorimaging ECL Plus Westerns visible fluorescence and gel documentation Typhoon FLA 7000 is intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Safety notices This user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of Typhoon FLA 7000 See definitions below
8. Found New llardware Wizard Click the Next button in the Found New Hardware Wizard dialog Insert the installation CD Select Install the software automatically Recommended Click the Next button in the Found New Hardware Wizard dialog Click the Finish button to complete the installation Install Typhoon FLA 7000 Control Software Windows XP Step Action 1 2 3 Insert the Typhoon FLA 7000 Control Software CD Locate and double click the file Typhoon FLA 7000 msi In the Typhoon FLA 7000 InstallShield Wizard click the Next button Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative see back cover of this manual for contact information Select I accept the terms in the license agreement and click the Next button Typhoon FLA 7000 User Manual 28 9607 62 AB 39 5 Installing Typhoon FLA 7000 Control Software 5 2 Install Typhoon FLA 7000 Control Software under Windows XP Step Action 5 Select destination folder in the dialog 180 sepale HA LOCC res al ie al Wier 1 Drstination Fairte Zara eee hve thteey e dr Tranh vette wits e Click the Next button to install the software at the default folder C Program Files e Click the Change button to install to a different folder 6 Click the Install button in the installation dialog 7 Click the Finish button to finish the installation of Typhoon FLA 7000 Control Software 5 3 Install Typhoon F
9. Should you find any external damages or if any items on the packaging list are missing notify the delivery company and contact GE Healthcare for further advice Store Typhoon FLA 7000 in an environment according to Appendix B Specifications on page 89 until the product is unpacked and installed Typhoon FLA 7000 User Manual 28 9607 62 AB 33 4 Installing and moving Typhoon FLA 7000 4 2 Transporting Typhoon FLA 7000 4 2 Transporting Typhoon FLA 7000 CAUTION Typhoon FLA 7000 must be secured before long distance transports Contact GE Healthcare for help and advice before transporting Typhoon FLA 7000 long distances The Typhoon FLA 7000 weighs approximately 62 kg and requires at least two persons to lift and move Precautions before moving the instrument Step Action 1 Turn off the Typhoon FLA 7000 2 Turn off the computer and any peripheral devices 3 Disconnect the power cords and the USB connection 4 3 Connections CAUTION Only authorized service personnel is allowed to install Typhoon FLA 7000 Contact your local GE Healthcare representative for help and advice Communication Step Action 1 Connect a USB cable to the USB port on the left side of the Typhoon FLA 7000 2 Connect the other end of the USB cable to a USB port on the computer CAUTION Do not connect any USB devices other than the Typhoon FLA 7000 to the computer in which the Typhoon FLA 7000 Control Software is install
10. Using Typhoon FLA 7000 comprises a series of steps outlined below Detailed explanations are provided in subsequent chapters Turn on the Typhoon FLA 7000 Phosphorimaging samples Start the computer and launch the Typhoon FLA 7000 Control Software Expose the storage phosphor screen Set the reading parameters Place the stage and the sample in the Typhoon FLA 7000 Read the sample 9 2 Preparations before starting Typhoon FLA 7000 Checklist before starting Typhoon FLA 7000 e Typhoon FLA 7000 is placed on a sturdy horizontal surface e Typhoon FLA 7000 is connected to a grounded wall outlet e The air intake fan on the rear side of Typhoon FLA 7000 is unobstructed e The air exhaust fan on the rear side of Typhoon FLA 7000 is unobstructed e There are no objects on top of Typhoon FLA 7000 Typhoon FLA 7000 User Manual 28 9607 62 AB 57 9 Operation 9 2 Preparations before starting Typhoon FLA 7000 e There is no stage in the stage rack 9 3 Starting Typhoon FLA 7000 and Typhoon FLA 7000 Control Software Start Typhoon FLA 7000 CAUTION Do not insert a storage phosphor screen in Typhoon FLA 7000 before turning on the machine If a storage phosphor screen is detected during the self diagnosis of the Typhoon FLA 7000 the sensitivity of the storage phosphor screen may deteriorate and reduce the quality of the scanned data Step Action 1 Push the power switch on the left side of the body to the I positio
11. image can also be saved in TIFF file format For TIFF files image data type is always set to Linear format Launch Applica Contains the path to the application used to analyze the data tion Select the analysis application by clicking the Select button Then browse to the software executable and click OK User account specific settings Changes to the settings in the Preferences dialog affect only the current Windows user account Changes made using a different user account do not affect the current user account Typhoon FLA 7000 User Manual 28 9607 62 AB 75 11 About storage phosphor screens 11 About storage phosphor screens 11 1 Introduction The Storage phosphor screen is a radiation energy memory type two dimensional sensor which has an image recording layer consisting of polyester base material densely coated with accelerated phosphorescent fluorescent material of fine crystals e Exposure A Storage phosphor screen accumulates and stores radiation energy while it is exposed It is exposed in close contact with an RI sample in a cassette like X ray film e Scanning The recording surface of an exposed Storage phosphor screen is scanned with a laser beam inside Typhoon FLA 7000 and emits fluorescent light according to the exposure level A photo multiplier tube PMT detects the fluorescent light and converts it into electric signals A radiation image recorded on the Storage phosphor screen during exposu
12. ina i loud Dutra E Alea Lelo BB Dotat cr ndonse haeret Explorer 3 HECPLLED Tal due D AANO BB soartei peri fer unea rena Pepene BERT Teldec L Ereren be rerum ciety serrer a 32B LRF er musa 49 SU mare Valder a scurta peste Fer vindos 2 Br 22080 3 Confirm the uninstallation by clicking Yes when prompted Note Correction files created during calibration such as shading data are required by the Typhoon FLA 7000 Control Software They are not deleted during the uninstallation and remain in the Data folder of Typhoon FLA 7000 Control Software folder Uninstalling Typhoon FLA 7000 Control Software under Windows Vista Step Action 1 Open the control panel and Select Uninstall a program under Programs Typhoon FLA 7000 User Manual 28 9607 62 AB 45 6 Uninstalling and upgrading Typhoon FLA 7000 Control Software Step Action 2 Select Typhoon FLA 7000 and then click Uninstall 753 o Control Panel Programs Programa and Features s search P Uninstall or change a program To uninstall a program elect it feom the list and then click Uninstall Change or Repair Organize Jil Views Cy Unites E Change Eh Repair e Name i Publaher Installed On IR Decyser 20 Database GE Healthcare 19 10 2009 Tec per 20 Oracte Server GE Heahhcare Gnome D DeCyder 2D Program GE Healthcare 6 10 2009 A imageManter 20 Platinum 7 GE Meatthcare 6 10209 ImageQuant
13. retrieved from the scanner Countermeasure Check that the scanner is properly connect ed to the computer then try to run the scan again Countermeasure Disconnect all other USB devices from the computer and run the scan again Countermeasure Restart the computer and Typhoon FLA 7000 Control Software then try to run the scan again Typhoon FLA 7000 User Manual 28 9607 62 AB 85 13 Troubleshooting Error message Meaning and countermeasure The stage is not properly inserted The stage is not properly inserted Please insert the stage in its cor rect position Countermeasure Insert the stage properly Errors and warnings in the scan progress window Error message Meaning and countermeasure Overexposure error occurred Set a lower PMT voltage The scanned image was overexposed due to a high PMT voltage setting Countermeasure Decrease the PMT voltage in the Reader Settings window Scanning stopped because the door was opened The door was opened during the scan Countermeasure Close the door and scan the image again Do not open the door until the scanning is fin ished The combination of the laser and filter might be inappropriate Check the laser and filter The selected method may be inappropriate Countermeasure Select another method or edit the method to suit the current application A scanner error was detected Please restart Typhoon FLA 7000 imager and Scan
14. set of stored conditions by selecting it in the list then click OK Remove a set of stored conditions by selecting it then click Remove Confirm the removal by clicking the Yes button in the next dialog box Set the pixel size for reading Pixel Size Click to select from one of the four types A sample with a C 25 um smaller pixel size is analyzed in finer detail 50 pm e 100 um 200 100 50 25 q Short Reading Time Long wo Small Image File Size Large This setting specifies the dynamic range The dynamic range that can be detected is larger with L5 than with L4 If the signals of the sample are in the L4 range the density gradation is represented in more detail if L4 is selected Select the stage which will be used in the scan The image under Sampling Area changes to reflect the selection Sampling area Displays the area or areas to be scanned as red boxes e Change the size of the area or areas to be scanned by clicking and dragging the edges of the red box with the mouse e Change the position of the area or areas to be scanned by clicking and dragging the red box with the mouse Typhoon FLA 7000 User Manual 28 9607 62 AB 71 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Mode Sets the selection behavior of Sampling area Setting Description Grid The sampling area is increased or decreased in steps corresponding to the grid of th
15. the power switch of Typhoon FLA 7000 Result The scanner will automatically be detected by the computer and the Plug and Play function in Windows Vista starts In the Found New Hardware dialog select Locate and install driver software recom mended Insert the Typhoon FLA 7000 Control Software CD when prompted and click the Next button in the Found New Hardware dialog Typhoon FLA 7000 User Manual 28 9607 62 AB 5 Installing Typhoon FLA 7000 Control Software 5 3 Install Typhoon FLA 7000 Control Software under Windows Vista Step Action 4 Select Install this driver software anyway we See dotado 5 A successful installation message appears Click the Close button Install Typhoon FLA 7000 Control Software Windows Vista Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if orompted then click Continue to proceed with the installation Step Action Insert the Typhoon FLA 7000 Control Software CD 2 Locate and double click the file Typhoon FLA 7000 msi 3 In the Typhoon FLA 7000 InstallShield Wizard dialog click the Next button 4 Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative See the back cover of this manual for contact information Select accept the terms in the license agreement and click the Next but
16. 0 and the computer Cleaning the stage CAUTION Wear gloves to prevent direct contact with chemical substances Step Action 1 Remove the stage from the main body of the instrument 2 Wipe the stage with a sponge moistened with a fluorescence free neutral detergent 3 Thoroughly rinse the stage with water and dry with a lint free cloth 4 Place the stage in the main body of the instrument Typhoon FLA 7000 User Manual 28 9607 62 AB 81 12 Maintenance Storing the Fluor or Multi stage Store the Fluor or Multi stage in the original packaging when they are not in use DO NOT store stages inside Typhoon FLA 7000 82 Typhoon FLA 7000 User Manual 28 9607 62 AB 13 Troubleshooting 13 Troubleshooting This chapter describes various problems that can foreseeably occur with the Typhoon FLA 7000 Control Software Suggestions of possible countermeasures are given Do the following if an error occurs Step Action 1 Take note of the error code and error message on the monitor 2 Turn off the power to Typhoon FLA 7000 and the computer then turn them on again after about ten seconds 3 Try to perform the action again If the error persists contact your GE Healthcare repre sentative General errors and warnings Error message Meaning and countermeasure Failed to open User Manual Please note that a PDF The software required to read the online reader e g Adobe Reader is needed PDF documentation i
17. 000 Placing the Fluor or Multi stage in the Typhoon FLA 7000 Step Action 1 Pull the knob up and open the lid 2 Position the stage so that the black triangle mark is aligned with the corresponding mark on the setting block Fit the setting block pin in the hole of the stage 3 Pullthe knob up and close the lid Typhoon FLA 7000 User Manual 28 9607 62 AB 65 9 Operation 9 7 Placing the stage in Typhoon FLA 7000 Placing the phosphor stage in Typhoon FLA 7000 Step Action 1 Pull the knob up and open the lid 2 Position the stage so that the black triangle mark is aligned with the corresponding mark on the setting block 3 Fit the setting block pin in the hole of the stage 4 Pull the knob up and close the lid 9 8 Scanning the sample Click the Start Scan button to start reading the sample The scanning progress window opens 66 Typhoon FLA 7000 User Manual 28 9607 62 AB 9 Operation 9 8 Scanning the sample Typhoon FLA 7000 Reader Scanning Gondition F Auto Range Scope tagntieston aa Whe Save AS fas Note If you click the Stop button during reading the part that has not been read yet will be saved as an image with a data value of 0 light intensity of 0 Note When you click the Stop button the reading is aborted You cannot start reading again from the location where reading stopped 9 9 Adjusting the display parameters Step Action 1 Adjust the display para
18. 07 n Name Type Date met Location gt Faes Current en the Disc 2 Recent Places USE Control E fe foder cont Ji USB Fecsction tea Typhoon FLA 7000 User Manual 28 9607 62 AB Select Show All Devices and click the Next button Fiom the Bat below select the type of hardware you are installing Insert the Typhoon FLA 7000 Control Software CD and click the Browse button Select to install the driver from the Typhoon FLA 7000 Control Software CD 41 5 Installing Typhoon FLA 7000 Control Software 5 3 Install Typhoon FLA 7000 Control Software under Windows Vista Step Action 11 Select the file DevMng and click the Open button Tioti E Lookin fy USE Cortes OF Po S de A z A 12 Click the OK button in the dialog Install from disk 13 Click the Next button in the wizard Add hardware 14 Click the Next button once again 15 The following warning is displayed Proceed by cliking Install this driver software anyway 16 Click the Finish button in the Add Hardware wizard to complete the installation Install the USB function driver Windows Vista 42 Note Step During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Action Connect the computer and the Typhoon FLA 7000 with a USB cable and turn ON
19. 3 2 Instrument body 3 2 Instrument body Power switch The power switch is located on the left side of the Typhoon FLA 7000 Inlet Cooling fans The instrument has two cooling fans on the back side CAUTION AN Do not block the cooling fans with a wall or objects If they are blocked the instrument may become damaged Typhoon FLA 7000 User Manual 28 9607 62 AB 25 3 System configuration 3 2 Instrument body Air intake fan Exhaust fan Indicator lamps Three indicator lamps indicate the instrument status POWER sus ennor 26 Typhoon FLA 7000 User Manual 28 9607 62 AB 3 System configuration 3 2 Instrument body Stage setting block Knob j St d Stage setting block age aoor Typhoon FLA 7000 User Manual 28 9607 62 AB 27 3 System configuration 3 2 Instrument body Filter module The filter module is located behind the filter door Filter module Filter module slot Filter change door 28 Typhoon FLA 7000 User Manual 28 9607 62 AB 3 System configuration 3 2 Instrument body USB port The USB port is located on the left side of Typhoon FLA 7000 USB label 3 3 Accessories Digitization plate included The digitization plate is a fluorescent plate that emits light when exposed to a laser beam This plate is used when the digitization mode is used Typhoon FLA 7000 User Manual 28 9607 62 AB 29 3 System conf
20. 8 cm x 36 cm valid field angle Fluorescent 24 cm x 40 cm maximum 22 cm x 36 cm valid field angle 238 mm x 398 mm setting size Digitization 24 cm x 40 cm maximum 22 cm x 36 cm valid field angle 238 mm x 398 mm setting size Parameter DJojro Pixel size 25 50 100 or 200 um selectable Gradation 16 bit Dynamic range Five orders of magnitude Typhoon FLA 7000 User Manual 28 9607 62 AB 89 B Specifications Parameter Maximum image capacity 307 2 MB 25 um 76 8 MB 50 um 19 2 MB 100 um 4 8 MB 200 um Dimensions amp weight Unit Dimensions mm wx Weight kg dxh Reading block 940 x 556 x 360 62 projections not includ ed Storage phosphor screen cassette 227 5 x 427 5 x 15 Approx 1 Phosphor stage 429 x 283 x 35 Approx 2 Fluor stage 429 x 283 x 35 Approx 1 Multi stage 429 x 283 x 33 Approx 1 3 FLA Image Eraser 603 x 512 x 163 14 5 Note The Reading block is a standard unit The other units are optional Power supply Parameter Data Input voltage 100 to 240V AC Allowable variations in volt 10 age Frequency 50 to 60 Hz Rated current 1 5 to 3 0 A Environmental conditions Parameter Operating conditions Temperature 15 to 30 C Humidity 30 to 70 no dew condensation 90 Typhoon FLA 7000 User Manual 28 9607 62 AB B Specifications Parameter DJojro Non operating co
21. GE Healthcare Typhoon FLA 7000 User Manual Table of Contents 1 Introduction Important user information Regulatory information The Typhoon FLA 7000 laser scanner Typhoon FLA 7000 Control Software 1 1 1 2 1 3 1 4 2 Safety instructions co fe Le q PRESSE RIO IER E General precautions Personal protection LaSer SOMEM rss cecossesscecansass RR OR RARI PREIA IRI 2 1 2 2 23 2 4 2 5 2 6 2 7 2 8 2 9 Radiation safety Radiation hazard prevention ERC Safety eserinin Labels on Typhoon FLA 7000 Emergency procedures 2 10 Recycling and disposal 3 System configuration 3 1 3 2 3 3 4 Installing and moving Typhoon FLA 7000 Site requirements Transporting Typhoon FLA 7000 COMME CU ONS toat meat i asia vot ein avale 4 1 4 2 4 3 5 Installing Typhoon FLA 7000 Control Software Installation sequence Install Typhoon FLA 7000 Control Software under Windows XP Install Typhoon FLA 7000 Control Software under Windows Vista 5 1 5 2 5 3 6 Uninstalling and upgrading Typhoon FLA 7000 Control Software 7 Registering editing and deleting methods Registering a new method Editing a method Deleting a method 7 1 7 2 7 3 8 Filter module settings Registering a new filter name Saving a filter combination Loading a filter combination Installing and replacing filters 8 1 8 2 8 3 8 4 Typhoon FLA 7000 User Manual 28 9607 62 AB Parts and functions Instrument body aeee e EE E Table of Contents Table
22. GE Healthcare 6140 2000 HE itm Graphics Media Accelerator Driver Intel Corporation 6710 2009 Dea esiR Network Connections 131 331 Intel 6 10 2000 betel TV Wizard Intel Corporation 812 2009 QT LSecustTy GE Healthcare 6 10 2009 PB Microsott NET Framework 4 Microsoft 6700 2000 1 Microsett NET Framework 3 5 SP1 Microsoft Cerperation 6 10 2009 REF Typhoon FLA 7000 GE Meahheare SAI 3 Confirm the uninstallation by clicking Yes when prompted 4 If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified program wants access to your computer Click Allow Upgrading Typhoon FLA 7000 Control Software 46 Step Action 1 Uninstall the current version of Typhoon FLA 7000 Control Software 2 Install the new version of Typhoon FLA 7000 Control Software Follow the instructions in the installation chapter of Getting Started Typhoon FLA 7000 User Manual 28 9607 62 AB 7 Registering editing and deleting methods 7 Registering editing and deleting methods Note Method settings are registered by a serviceman upon installation Under normal circumstances it is not necessary to change these settings 7 1 Registering a new method Step Action 1 Click the Method button in the main window The Method Settings dialog box appears Method Settings Method List Method Name 7 Laser Fiter Name F 310580 532nm s 0580 s35nm BBIR670 3635nm BBIR670
23. LA 7000 Control Software under Windows Vista Before you begin Log in using a Windows account with administrator privileges Install the USB Control Driver Windows Vista Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Step Action 1 Disconnect Typhoon FLA 7000 Control Software from the computer 2 Open the control panel and click Classic View in the upper left corner 3 Open Add Hardware 4 In the Add Hardware dialog click the Next button 5 Select Install the hardware that I manually select from a list Advanced and click the Next button 40 Typhoon FLA 7000 User Manual 28 9607 62 AB Step Action 5 Installing Typhoon FLA 7000 Control Software 5 3 Install Typhoon FLA 7000 Control Software under Windows Vista 6 Wt you do net see the hardware category you mart chick Show Al Devices Common hardware typer By Digi sector Eq IDE ATA ATAPI coensoiers ii IEEE 1394 Bus host contokers Imaging devers ze dences Maka Cet E tarm Gsi Cosa 7 Click the Have Disk button uan 6 DORN Om prennan O 9 gt E Location te medi Recent Places Pi E Compete baa Bake 10 Select the USB Control folder and click Open lok DVO AW Deve D Typhoon FLA NOO 09
24. LA 7000 is in self diagnosis Please wait Ready The unit is ready to use 9 4 Selecting the reading mode Click the button that corresponds to the desired reading mode 60 Typhoon FLA 7000 User Manual 28 9607 62 AB 9 Operation 9 4 Selecting the reading mode To Click this button Read a fluorescent sample amp Ei Eivoruocanco Read a phosphorimaging sample E menu Be Read a digitization sample oigtaston 9 5 Setting the reading conditions Set the reading conditions by following the instructions in Chapter 10 Reading conditions display parameters and other settings on page 69 9 6 Placing the object to be scanned on the stage CAUTION Always wear gloves protective glasses and a lab coat or similar when handling samples Placing a gel sample on the Fluor stage Note e The sample must not be thicker than 30 mm For solutions the maximum height is 4 mm e Make sure there are no bubbles or gaps on the contact surface Step Action 1 Place the sample on the Fluor stage Typhoon FLA 7000 User Manual 28 9607 62 AB 61 9 Operation 9 6 Placing the object to be scanned on the stage Placing a titer plate on the Multi stage Step Action 1 Place the titer plate frame on the Multi stage j af miy A Bic ioi elrialnii ylikiLi uiniole 2 Place the titer plate in the desired position in the titer plate frame GY eesnime orare za re
25. SITION AU RAYON CAUTION Class 3B Laser product when open and interlock defeated Avoid exposure to the beam Do not attempt to defeat the safety interlocks under the sample lid or behind the filter door or otherwise try to gain access to the interior of the instrument through any other opening Exposure to laser light can cause injury Viewing the laser light directly can cause blindness 18 Typhoon FLA 7000 User Manual 28 9607 62 AB Labels at the front of Typhoon FLA 7000 1 21V5 DEY F R FLA 7000B AOD AIRS CSCMOFIRCSCED TCE 2Q ITMIEVA WECVYES SIE ARE E CHER ICFLA 7000K ANELAA THEO 1 Please set or exchange filter as directed in operation manual 2 Please insert the filter module into FLA 7000 firmly till it reaches the end 2 Safety instructions 2 8 Labels on Typhoon FLA 7000 ER TTERNT AVI OD EMIRIBEOTAIB Ei RUT TENTE LORE ERII KIER CAUTION CLASS 3B VISIBLE AND INVISIBLE LASER RADIATION WHEN OPEN AND INTERLOCKS DEFEATED AVOID EXPOSURE TO THE BEAM ATTENTION RAYONNEMENT LASER VISIBLE ET INVISIBLE DE CLASSE 3B A L OUVERTURE ET QUAND LE VERROUILLAGE EST DEJOUE EVITER L EXPOSITION AU RAYON DAZ VIEAADE VITHRICELAGESICVY FUTCKKESEU QVREAF VICLY F CSSYUIWVOEASImMMUAFCS 1 Please insert the stage so that it fits pins on the loading section firmly 2 Sample thickness on the fluor stage is 30mm or less Typhoon FLA 7000 User Manual 28 9607 62 AB 19 2 Safety in
26. Typhoon FLA 7000 Control Software 5 2 Install Typhoon FLA 7000 Control Software under Windows XP Action 10 11 Select Add a new hardware device and click the Next button dalil Hea urari Wize Thy fulluwiny headway e eos indad uri yuu computer sirmity tina yter mh ee her are taie Fak mgn te elindacrala m ul ku Ti Te sd Farhuare st aber riw acd aren wrewes derie sealed pe doare Sy JSD ox lt SE aa sase Jon e in Hut carmel bear Ia Select Install the hardware that manually select from a list Advanced and click the Next button Select Show All Devices and click the Next button dubil Hea alura Wizard From tvs ligt Loco pulusi ihe ype of han puu ave inelaliriy ya cand see bre atat caber rascal due shoe Lene Terre g8icna s types Click the Have Disk button in the Add hardware wizard Insert the Typhoon FLA 7000 Control Software CD and click the Browse button Select to install the driver from the Typhoon FLA 7000 Control Software CD Lacate Lile ozki EIS Zorputzi ee Loca B a Rezart Jocumente rym ey STAR Bare My Compute sra en aq ce SICFENGO E CaS at Cucu eds 3 My Dozunerts Sy He atok Places He zone DosM g inl lies al ne Typhoon FLA 7000 User Manual 28 9607 62 AB 37 5 Installing Typhoon FLA 7000 Control Software 5 2 Install Typhoon FLA 7000 Control Software under Windows XP Step Action 12 Open the USB Control folder Lacate Lile
27. a SUKI slegge a E E ms 1 Li S 0 ERT S E E a OD ID E miniote Placing a gel sample with glass on the Multi stage Step Action 1 Place a gel sample with glass on the Multi stage 62 Typhoon FLA 7000 User Manual 28 9607 62 AB 9 Operation 9 6 Placing the object to be scanned on the stage Step Action 2 Carefully fold down the spring loaded glass holders on the glass Placing the storage phosphor screen on the phosphor stage CAUTION Always wear cotton gloves when handling storage phosphor screens Step Action 1 Turn down the lighting in the room Typhoon FLA 7000 User Manual 28 9607 62 AB 63 9 Operation 9 6 Placing the object to be scanned on the stage Step Action 2 Place the cassette with the exposed storage phosphor screen next to the phosphor stage 4 Press one end of the suction rod against the storage phosphor screen Cover the other end of the suction rod with a finger Lift up the storage phosphor screen by the suction rod and place the storage phosphor screen on the phosphor stage Release the storage phosphor screen by releasing your finger from the suction rod 4 Place the storage phosphor screen on the back of the phosphor stage with the white or blue reading surface of the storage phosphor screen facing up 64 Typhoon FLA 7000 User Manual 28 9607 62 AB 9 Operation 9 7 Placing the stage in Typhoon FLA 7000 9 7 Placing the stage in Typhoon FLA 7
28. abels on left side of Typhoon FLA 7000 Ra TOEBICLOSASB OURAROFTRADWES E LORIE lt E RUF TRIE CAUTION CLASS 38 VISIBLE AND INVISIBLE LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM ATTENTION RAYONNEMENT LASER VISIBLE ET INVISIBLE DE CLASSE 3B A L OUVERTURE EVITER L EXPOSITION AU RAYON 2 Safety instructions 2 8 Labels on Typhoon FLA 7000 2 9 Emergency procedures This section describes how to do an emergency shutdown of the Typhoon FLA 7000 instrument The section also describes the results of a power failure Emergency shutdown Step Action 1 Click the Stop button in the Typhoon FLA 7000 Control Software 2 Turn off the Typhoon FLA 7000 by pressing the power switch on the left side of the instru ment body 3 Disconnect the power cord from the power outlet Typhoon FLA 7000 User Manual 28 9607 62 AB ei 2 Safety instructions 2 9 Emergency procedures Power failure The results of a power failure depends on the unit or units affected Unit affected by power failure Results Typhoon FLA 7000 instrument e The reading is interrupted immediately The instrument is in an undefined state e The data collected up to the time of the power failure is available in the file created when starting the scan Computer running the Typhoon FLA e The computer shuts down immediately 7000 Control Software e The run continues but no data is saved 2 10 Recycling and disposal Ge
29. ading conditions explained Method Set the method to use in the scan From the pull down menu select the method that corre Method Cy5 sponds with the sample The selected laser and filter com Cy3 bination is displayed below the menu EtBr ROX Deep Purple Pro Q Diamond Cy5 Alexa Fluor 633 DDAQ Silver Stain CBB X In fluorescent mode up to 4 scans can be performed in a row all with individual methods PMT PMT Fin v 6500 1200 You may set the voltage to be applied to the photo multi plier tube PMT as an integral value within the predeter mined range The larger the value is the higher the sensi tivity Plus and minus buttons fluorescent mode only Click to increase the number of scans Click to decrease the number of scans Top button Click this button to return to the main window Save condition Click this button to save the current conditions settings to a file Save the current conditions by clicking Save Condition A text dialog opens Enter a name for the conditions in the text field and click OK to save 70 Typhoon FLA 7000 User Manual 28 9607 62 AB 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Load condition Pixel size Latitude Stage Click this button to load previously saved conditions Clicking the button opens a dialog box with a list of previously saved condition sets Load a
30. ck the magnifying glass symbol and right Click the magnifying glass symbol and left click click the display area the display area or or select a magnification ratio further down the select a magnification ratio further up the Mag Magnification drop down list nification drop down list Intensity graph The intensity graph displays the intensity levels of the image in histogram format The Low and High values below the graph correspond to the range of light intensities displayed These values are displayed in the graph as vertical red lines To display a larger or smaller interval of light intensities drag the lines using the mouse to adjust the Low and High values Curve Select the type of tone curve used in the intensity graph Setting Description Exponential The light intensities are displayed using an exponential tone curve Linear The light intensities are displayed using a linear tone curve Sigmoid The light intensities are displayed using a sigmoid S shaped tone curve Auto Range Scope Check this option to automatically adjust the range of light intensities for optimum display results Typhoon FLA 7000 User Manual 28 9607 62 AB 73 10 Reading conditions display parameters and other settings 10 3 Other settings 10 3 Other settings Preferences gt Preferences Click the Preferences button in the main window to display a tabbed dialog box where various options related to samp
31. cted by the computer Countermeasure Check that Typhoon FLA 7000 is properly connected Follow the instructions in Chapter 4 Installing and moving Typhoon FLA 7000 on page 33 Typhoon FLA 7000 imager is run ning self diagnosis mode Please wait The self diagnosis of Typhoon FLA 7000 is running Countermeasure Wait until the self diagnosis is finished the proceed with the scan A laser error was detected Please retry the scan or contact service There was an error with the laser Countermeasure Try to scan again Countermeasure Restart the instrument and Typhoon FLA 7000 Control Software then try to run the scan again Countermeasure Scan using a different laser Please select image folder No image folder is selected Countermeasure Select an image folder by clicking the Browse button and navigating to a suitable folder Please input a file name No name has been assigned to the image file Countermeasure Type a name in the File Name field Please set PMT Voltage value to 500 1000 The PMT voltage setting is outside the permitted range Countermeasure Type a PMT voltage value between 500 and 1000 in the PMT field Please give a name to the condi tion No name was given to a condition before saving Countermeasure Type a name in the Condition Name field Failed to retrieve image data from the scanner Check the scanner connection The image data could not be
32. den Name and ID of notified body INTERTEK SEMKO AB NB 0413 Place and date of declaration Uppsala Sweden Sept 2009 Identity of person authorized to sign Declaration See EC Declaration of Conformity of Conformity 6 Typhoon FLA 7000 User Manual 28 9607 62 AB International standards This product fulfills the requirements of the following standards Standard EN 61010 1 IEC 61010 1 UL 61010 1 IEC 61010 2 81 CAN CSA C22 2 No 61010 1 Description Safety requirements for electrical equipment for measurement control and laboratory use 1 Introduction 1 2 Regulatory information Notes EN 61326 1 VCCI Class A FCC Part 15 B Class A ICES 003 Class A EMC emissions and immunity require ments for electrical equipment for measurement control and laboratory use Harmonized with 2004 108 EC EN ISO 12100 1 12100 2 Safety of machinery Basic concepts general principles for design Harmonized with 2006 42 EC EN ISO 14121 1 14121 2 Safety of machinery Principles of risk assessment Harmonized with 2006 42 EC EN 60825 1 IEC 60825 1 Safety of laser products Harmonized with 2006 95 EC USA 21 CFR Chapter Subchapter J Part 1040 10 Laser Products Safety of laser products CE Conformity This product complies with the European directives listed in the table by fulfilling the corresponding harmonized standards A copy of the Declaration of C
33. e stage or the positions of the titer plate plugin Free The sampling area is increased or decreased in a stepless fashion All The sampling area is expanded to the whole readable surface of the stage or titer plate plugin is selected File Size This area displays the estimated size of the image file as determined by the current settings Reading Time This area displays the estimated reading time as determined by the current settings Scan button Click this button to start scanning the sample or samples using the current reading conditions 10 2 Display parameters explained Upper limit Lower limit Intensity graph Low 10600 High 40600 ro Raed Curve F Auto Range Scope Auto Range Scope Magnification _ _ stenn a i 4 re Soweto eee ee pe E Return File Format Get 72 Typhoon FLA 7000 User Manual 28 9607 62 AB 10 Reading conditions display parameters and other settings 10 2 Display parameters explained Magnification Select a ratio to zoom in and out of the display area If the magnification is high scroll bars appear on the right and bottom edges of the display area Use these scrollbars to view different parts of the display area There are several ways to zoom in and out of the display area see the table below Zooming in Zooming out Click the magnifying glass symbol and left click Click the magnifying glass symbol and right the display area click the display area or or Cli
34. ed Useonlythe Typhoon FLA 7000 Control Software during reading 34 Typhoon FLA 7000 User Manual 28 9607 62 AB 4 Installing and moving Typhoon FLA 7000 4 3 Connections Electrical power Connect the power cord of the Typhoon FLA 7000 to a grounded power outlet WARNING Use only power cords delivered or approved by GE Healthcare Typhoon FLA 7000 User Manual 28 9607 62 AB 35 5 Installing Typhoon FLA 7000 Control Software 5 Installing Typhoon FLA 7000 Control Software Administrator privileges required All tasks related to software installation or uninstallation require a computer account with administrator privileges 5 1 Installation sequence Software installation is performed in the following sequence 1 Install the USB control driver 2 Install the USB function driver 3 Install the Typhoon FLA 7000 Control Software 5 2 Install Typhoon FLA 7000 Control Software under Windows XP Before you begin Log in using a Windows account with administrator privileges Install the USB Control Driver Windows XP Step Action 1 Disconnect Typhoon FLA 7000 from the computer 2 Open the control panel and select Printers and Other Hardware 3 Click Add Hardware to open Add hardware wizard 4 Click the Next button in Add hardware wizard 5 Select Yes have already connected the hardware and click the Next button 36 Typhoon FLA 7000 User Manual 28 9607 62 AB Step 5 Installing
35. ents e f water enters the Storage phosphor screen the sensitivity of the storage phosphor screen is reduced e Volatile solvents may deform the protective film on the storage phosphor screen Protect exposed storage phosphor screens from light Protect exposed storage phosphor screens from light until the reading is finished Store the storage phosphor screen correctly Remove the storage phosphor screen from the Typhoon FLA 7000 main unit when not in use Store the storage phosphor screens in a horizontal position protected from moisture Typhoon FLA 7000 User Manual 28 9607 62 AB 77 11 About storage phosphor screens 11 4 Preparing the storage phosphor screen 11 4 Preparing the storage phosphor screen Procure necessary tools and items Prepare by acquiring the following items Storage phosphor screen cassette e Radio isotope labeled samples e Gloves e Plastic film e Soft lint free tissues e Ethanol Clean the storage phosphor screen and the cassette Clean the surface of the storage phosphor screen and the inside of cassette with a soft tissue to remove dust and stains Erase the storage phosphor screen The FLA Image Eraser can erase the storage phosphor screen in around 15 minutes if it is not overly exposed For details refer to the FLA Image Eraser User Manual Dim the lighting e Dim the ambient light to 20 lux or less before opening or moving the storage phosphor screen without a cover
36. erique de la class A est conforme a la norme NMB 003 du Canada Note This is a class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures Laser standards This instrument is a Class 1 IEC60825 1 2001 laser product GE Healthcare Bio Sciences AB 751 84 Uppsala Sweden FLUORESCENT IMAGE ANALYZER MODEL Typhoon FLA 7000 SERIAL No MANUFACTURED FFTPT Complies with FDA performance standards for laser products except for deviations pursuant to Laser Notice No 50 dated June 24 2007 405N101499 The following lasers can be installed in Typhoon FLA 7000 Laser and class Wavelength Maximum power LD laser class 3B 473 nm 20 mW CW SHG laser class 3B 532 nm 10 mW CW LD laser class 3B 635 nm 45 mW CW LD laser class 3B 650 nm 80 mW CW 8 Typhoon FLA 7000 User Manual 28 9607 62 AB 1 Introduction 1 2 Regulatory information Regulatory compliance of connected equipment Any equipment connected to the Typhoon FLA 7000 should meet the safety requirements of EN 61010 1 IEC 61010 1 or relevant harmonized standards Within EU connected equipment must be CE marked 1 3 The Typhoon FLA 7000 laser scanner The compact Typhoon FLA 7000 is an image analyzer that offers high performance multifunctionality and high speed reading Benefits include e Detection of high sensitivity autoradiogra
37. escent pigment label samples non RI method Therefore it is recommended that the user should install it outside the controlled area and use Rl indicated samples without contacting them with Storage phosphor screens directly However as described above the Storage phosphor screen surface may be polluted by radioisotope RI depending on the sample condition since the instrument sticks the sample to the H compatible Storage phosphor screen surface and exposes it in an auto radiography experiment of the H label sample When a sample is in direct contact with a Storage phosphor screen it is generally known that the sample for making an auto radiogram contains a relatively small quantity of radioisotope However the degree of superficial pollution of the Storage phosphor screen is greatly influenced by the dryness of the sample and dose of radioisotope in an experiment and may exceed the limits mentioned in Limit of superficial pollution on page 15 When the instrument reads a Storage phosphor screen with a polluted non exposure area it may be polluted The degree of such superficial pollution greatly differs with users operation conditions Superficial pollution may exceed the limit mentioned above Note As mentioned above install this instrument in the RI controlled area if the user uses Rl indicated samples that will be in direct contact with Storage phosphor screens Removal from the controlled area If it is necessary to move the i
38. hanges by clicking the OK button in the Method Settings dialog box Note To undo the filter deletion click the Cancel button in the Method Settings dialog box Click Yes in the dialog box that appears see below Typhoon FLA 7000 User Manual 28 9607 62 AB 8 Filter module settings 8 Filter module settings Introduction Filter module settings are registered by a serviceman upon installation Under normal circumstances it is not necessary to register these settings When a filter is changed in the filter module of Tyohoon FLA 7000 the new or changed filter must be registered in the Typhoon FLA 7000 Control Software A filter which has not been registered can not be used for analysis and is not displayed in the Typhoon FLA 7000 Control Software 8 1 Registering a new filter name Step Action 1 Click the Filter Module button in the main window Qj Fiter Module 2 Click the Add button The following dialog box appears 3 Type the filter name in the Name field 4 Select an icon in the Icon drop down list 5 Finalize the filter addition by clicking the OK button 6 Click the OK button to return to the main window 8 2 Saving a filter combination Step Action il Click the Filter Module button in the main window U Fitter Module Typhoon FLA 7000 User Manual 28 9607 62 AB 51 8 Filter module settings 8 2 Saving a filter combination Step Action 2 Click the Save Filter Module button 3 T
39. iguration 3 3 Accessories Fluor stage included The Fluor stage is used to read gels or membranes e Place the gel or membrane directly on the glass of the Fluor stage e Place the Fluor stage in the instrument Glass surface Membrane weight included The membrane weight is used to hold thin fluorescent samples flat against the Fluor stage gt S Multi stage included The Multi stage is used to read gels with glass gel merely supported by glass or titer plates e Place the gel with the glass plate directly on the Multi stage or place a titer plate on the titer plate plugin attached to the instrument e Place the titer plate on the Multi stage 30 Typhoon FLA 7000 User Manual 28 9607 62 AB 3 System configuration 3 3 Accessories Phosphor stage included The phosphor stage is used when reading storage phosphor screens The stage is magnetic to hold storage phosphor screens e Place the phosphor stage upside down on a flat surface e Place the storage phosphor screen on the phosphor stage with the reading surface facing away from the phosphor stage e Place the protective cover on top of the storage phosphor screen e Flip the phosphor stage over e Place the phosphor stage in Typhoon FLA 7000 Storage phosphor screen sticking side Power cord included Typhoon FLA 7000 uses a special AC power cable Be sure to use power cables specified in service manuals or by service per
40. le reading can be viewed and changed Not all options are relevant in all scanning modes Gel Image Fie 92 Gch Image Fie gel TIFF Image File 41 Launch Applicator r E Setting Description Correction Mode Auto Use specific image shading correction data that were adjusted in accor dance with each laser Manual The correction method must be selected individually in the Reader Settings window Note The correction mode settings list is registered by a serviceman Please contact the dealer where you purchased Typhoon FLA 7000 or contact GE Healthcare ND Filter Enables the use of an ND filter to adjust the light intensity Use the ND filter if the signal from the sample is very strong Select On to enable the ND filter Select Off to disable the ND filter Scan Mode Determines the scanning speed Standard Mode 200 um 210 s 100 um 210 s 50 um 330 s 25 um 450 s Quick Mode 200 um 150 s 100 um 150 s 50 um 210 s 25 um 330 s 74 Typhoon FLA 7000 User Manual 28 9607 62 AB 10 Reading conditions display parameters and other settings 10 3 Other settings Setting Description File Format Determines the file format used to save the reading data Gel Image File gel The standard file format is a gel file which contains square root encoded pixel data Gel Image File gel Tiff Image File tif In combination with a gel file a read
41. meters if desired Follow the instructions in Section 10 2 Display parameters explained on page 72 2 Click Return to return to the Reader Settings screen Typhoon FLA 7000 User Manual 28 9607 62 AB 67 9 Operation 9 10 Saving the image data using a different file name 9 10 Saving the image data using a different file name Step Action 1 After scanning the sample click the Save as button The following dialog opens Savein Typhoon FLA 700 a e ae File name Save as type Gai Image File gel Cancel Z TIFF image Fie i 2 Type a file name in the File name field 3 Select a file format in the Save as type drop down list 4 Save the image file by clicking the Save button 9 11 Viewing the image file in the analysis application Click the Launch button in the to open the image file in the registered analysis application Note Ifno analysis application is registered in Typhoon FLA 7000 Control Software the Launch button is greyed out and inactive If desired register or change the analysis application 9 12 Turning off Typhoon FLA 7000 Step Action 1 Turn off Typhoon FLA 7000 by pressing the power switch on the left side of the instrument body 68 Typhoon FLA 7000 User Manual 28 9607 62 AB 10 Reading conditions display parameters and other settings 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Save co
42. n 58 Typhoon FLA 7000 User Manual 28 9607 62 AB Step Action 9 Operation 9 3 Starting Typhoon FLA 7000 and Typhoon FLA 7000 Control Software 2 When the power switch is turned on the indicator lamps change as shown below POWER susv ERROR 4 POWER use 2 ERROR Pee 4 POWER eusr ERROR After turning on the power swich During self diagnosis or startup adjustment Ready for operation Start Typhoon FLA 7000 Control Software Step Action 1 Make sure that Typhoon FLA 7000 has completed the warm up after which only the power lamp on the upper left panel on the front of the Typhoon FLA 7000 is lit 2 Start Typhoon FLA 7000 Control Software from the Start menu or use the desktop shortcut Typhoon FLA 7000 User Manual 28 9607 62 AB 59 9 Operation 9 3 Starting Typhoon FLA 7000 and Typhoon FLA 7000 Control Software Step Action 3 The main window of the Typhoon FLA 7000 Control Software is displayed Sn i FLA 7000 ET Mode Settings o E Typhoon FLA 7000 tor Es imagination at work 2009 GENERAL ELECTRIC COMPANY ALL RIGHTS RESERVED Ve 5 es comicontact The condition is displayed in the Status area of the main window Status messages are as follows Message Explanation Disconnected Cannot recognize Typhoon FLA 7000 Please check connection and power Warm up Typhoon F
43. nder the equipment Using damaged power cords could result in fire or electric shock If the power supply cords are damaged contact your local GE Healthcare representative for replacements WARNING Do not place the equipment on unstable tables or on inclined surfaces as the equipment could be dropped or fall resulting in injury Typhoon FLA 7000 User Manual 28 9607 62 AB 13 2 Safety instructions 2 3 Personal protection 2 5 Personal protection A CAUTION Always wear gloves protective glasses and a lab coat or similar when handling samples A CAUTION Always wear cotton gloves when handling storage phosphor screens 2 4 Laser safety WARNING Never detach the inner cover screwed to this instrument If it is detached laser beam may leak with a risk of loss of vision A A CAUTION Using procedures or adjustments other than those specified in this manual may result in hazardous exposure to laser radiation 2 5 Radiation safety Radiation safety This instrument is not equipped with any radioisotope RI or radiation generating unit and is therefore not regulated by radiation hazard prevention laws However the instrument is capable of reading Storage phosphor screens which may be polluted by radioisotopes RI A CAUTION If radioisotope RI pollution occurs stop use of the instrument immediately and follow the instructions of your radiation administ
44. ndition Load condition Image folder Condition File Save Condition Load C ndition Image Folder C Program Files GE Healthcate Typhoon FLA 7000 Data Browse File Name File Name escsira Comment Pixel size Comment Reader Settings Fluorescence ste E Ras Latitude um cL4 n Method N 400 bm ots Latitude pur 500 00 1000 ED Stage Fones Stage PMT LIM N O P Sampling area Mode B c D E FIGIH i J K L A File Size about 10 30 M8 File Size Reading Time about 1m45s Top File Format Gel Reading Time Top ND Filter Off O Start Scan Scan Mode Standard Start Scan Specify where to save the file after the reading Note When reading more than once and reading of 1ch 2ch is implemented simultaneously a folder with a name set by File Name is created automatically at the specified position and the image data will be saved in the folder File Name Enter the name of a file to save image data This field is mandatory in order to start reading a sample Note The method name is automatically added to the specified name and is saved to a file Comment Enter an optional comment The comment is embedded in the file where the image is saved and can be viewed with the analyzing software Typhoon FLA 7000 User Manual 28 9607 62 AB 69 10 Reading conditions display parameters and other settings 10 1 Re
45. nditions Temperature 10 to 40 C Humidity 20 to 70 no dew condensation Transportation amp storage Temperature 25 to 70 C conditions within 96 hours Humidity 10 to 80 no dew condensation Heat radiation 151 W h reader block FLA Image Eraser Lighting It is recommended to lower the lighting level to about 20 lux when moving a sample from the cassette onto the Phosphor stage after exposure 20 lux is the brightness level at which most people can read newspaper sized text after he she becomes accustomed to the brightness Location of use Indoor use only Maximum altitude for use 2 000 m above sea level Overvoltage category Transient overvoltage category II Applicable Pollution Rating Pollution Degree 2 Noise levels Parameter Noise 70 dB A or lower Degrees of protection provid IP20 ed by enclosure Typhoon FLA 7000 User Manual 28 9607 62 AB 91 For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com quantitative_imaging imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company Typhoon is a trademark of GE Healthcare companies All third party trademarks are the property of their respective owners 2009 General Electric Company All rights reserved First published Oct 2009 All goods and
46. ner Control Software Sense Key 1H Error Code 2H 3H An error occurred during scanning Countermeasure Try to run the scan again Contact your GE Healthcare representative if the problem per sists 86 Typhoon FLA 7000 User Manual 28 9607 62 AB A Default sample detection methods Appendix A Default sample detection methods 473 nm laser Method name Filter type Cy 2 Y520 FITC Y520 SYBR Green Y520 SYPRO Ruby 0580 SYPRO Orange Y520 FAM Y520 Alexa Fluor 488 Y520 Digitize473 Y520 532 nm laser Method name Filter type Cy3 0580 EtBR 0580 ROX 0580 Deep Purple 0580 Pro Q Diamond 0580 Digitize532 0580 635 nm laser Method name Filter type Cy5 R670 Alexa Fluor 633 R670 Typhoon FLA 7000 User Manual 28 9607 62 AB 87 A Default sample detection methods Method name Filter type DDAO R670 650 nm laser Method name Filter type Phosphorimaging IP CBB R710 88 Typhoon FLA 7000 User Manual 28 9607 62 AB B Specifications Appendix B Specifications Wavelengths supported e 473nm e 532nm e 635nm e 650nm Filter types supported IP imaging plate included 0580 included R670 included Y520 included R710 optional accessory Scanning specifications Reading mode Scanned image size Storage phosphor screen 20 cm x 40 cm maximum 1
47. neral instructions for disposal When taking the Typhoon FLA 7000 out of service the different materials must be separated and recycled according to national and local environmental regulations Specific instructions for disposal Measure the superficial radio isotope pollution of the instrument body and storage phosphor screen as mentioned in the radiation hazard prevention section of the User Manual If the pollution level exceeds the limit dispose of the instrument body as radioactive waste Otherwise dispose of the materials according to applicable laws and regulations for disposal of industrial waste 22 Typhoon FLA 7000 User Manual 28 9607 62 AB 3 System configuration 3 System configuration 3 1 Parts and functions The parts and their functions are explained separately in this chapter Part Function 1 Power switch located between the AC connector and the USB connector 2 Indicator lamps Typhoon FLA 7000 User Manual 28 9607 62 AB 23 3 System configuration 3 1 Parts and functions Part Function 3 Knob 4 Lid 5 Setting block 6 Filter change door 7 Filter module slot 8 Filter module 9 Filter 10 Phosphor stage 11 Suction rod 12 Protection cover for storage phosphor screen 13 Fluor stage 14 Digitizing fluorescent plate 15 Membrane weight included in Fluor stage 24 Typhoon FLA 7000 User Manual 28 9607 62 AB 3 System configuration
48. ng the storage phosphor screen 80 Step Action 5 Place the storage phosphor screen in the cassette with the exposure surface of the storage phosphor screen facing the sample Make sure that the notch of the storage phosphor screen is in the front left corner of the cassette as shown below 6 Close the cover of the cassette Press on the cover until it clicks into place Typhoon FLA 7000 User Manual 28 9607 62 AB 12 Maintenance 12 Maintenance Cleaning the outside of Typhoon FLA 7000 Clean the outside with a moist soft cloth and a mild detergent Wipe afterwards with a dry soft cloth Maintenance of the SHG laser If you use the SHG laser in the Typhoon FLA 7000 it will require periodical calibration When the Typhoon FLA 7000 is switched on it automatically executes calibration You do not need to do a manual calibration if the Typhoon FLA 7000 is used at least once a month CAUTION AN Activate the Typhoon FLA 7000 at least once every 30 days to execute calibra tion This calibration ensure that the SHG laser operates properly Failure to calibrate the Typhoon FLA 7000 every 30 days reduces the lifespan of the SHG laser Operation procedures 1 Turn on the Typhoon FLA 7000 and the computer 2 Wait until warm up and self diagnosis is completed and the scanner is ready Only the power lamp on the upper left panel is lit The automatic calibration has now been performed 3 Turn off the Typhoon FLA 700
49. nstrument and its laboratory which were installed and have been used in the controlled area from the controlled area make sure that the degree of the superficial pollution is below the limits mentioned in Limit of superficial pollution above Typhoon FLA 7000 User Manual 28 9607 62 AB 15 2 Safety instructions 2 7 Electric safety 2 7 Electric safety WARNING Do not use the equipment with a power supply other than that recommended Fire and electric shock could result WARNING Do not use the equipment within or near a sink or in humid or dusty environments Fire and electric shock could result WARNING Connect the power supply directly to a grounded wall power outlet The use of extension cords or multiple loads on one electrical outlet could result in fire and electric shock CAUTION Do not use the same power supply as that of large equipment such as an air conditioner or centrifuge Malfunction could result Typhoon FLA 7000 User Manual 28 9607 62 AB 2 Safety instructions 2 8 Labels on Typhoon FLA 7000 2 8 Labels on Typhoon FLA 7000 Typhoon FLA 7000 serial number The Typhoon FLA 7000 serial number is located on a label on the back of the instrument GE Healthcare Bio Sciences AB 751 84 Uppsala Sweden FLUORESCENT IMAGE ANALYZER Model Typhoon FLA 7000 Serial No 123456 Frequency 50 60 Hz Supply Voltage 100 240 V Cc US Max Current 3 0 1 5A LISTED Protecti
50. of Contents 10 11 12 13 OF ENG e acs RE Ra EI RE DE SR E RS DTD SR a SS 57 91 Operation eh VA NE ana 00 iasa n E a a ala R 57 9 2 Preparations before starting Typhoon FLA 7000 assim nenea aaa 8 aaa 57 9 3 Starting Typhoon FLA 7000 and Typhoon FLA 7000 Control Software sitter 58 g4 SPS CU the redding MOTE ARON RIO RR RI NI ORARE INI 60 9 5 Setting the reading Conditions sosesc man mite i aie pei a ac nenna 61 9 6 Placing the object to be scanned on the stage sem css caiac aia a e dal a oz ea 61 9 7 Placing the stage in Typhoon FLA 7000 spas doc a aa cat cat 65 98 erste Ja pla e Ja CAME ROI IRI SRR RR re RER RR N CI ROI IE RR RE N RR NE RR 66 So Adjusting the display parameters acas doi anni ai ina nea ne 67 9 10 Saving the image data using a different file NAME ane anna aia anda 68 9 11 Viewing the image file in the analysis application nenea 68 Se Turni off Typhoon FLA 7 QQQ psasi sas peesos assinadas saca 880 caseta p cate cip tata bete asa 68 Reading conditions display parameters and other settings ccscscssecsccssseseceecssecessceecscsccceceees 69 10 1 Reading conditions explained osoasa i botu ata a aa a af n la 69 10 2 Display parameters explained eee asa e ceai dat ca a E A da d ta 72 10 3 Car po ss O IRIS RE NE eave eevee RR 74 Abo t Storage phosphor SCreenS ua poi sasa taiata dai cane an aaa de le dul pie e aan nada 76 Pta PA Ua dpe fe la o N eee tetera adrienne O E A a ca MR Nae e e RIA 76
51. on Class IP20 LABORATORY EQUIPMENT Manufacturing Year ACESO Manufacturer FUJIFILM Corporation TOKYO 106 8620 JAPAN MADE IN JAPAN 405N101498 Symbols used in safety labels Label Meaning Warning Read the user documentation before using the system Do not open any covers or replace parts unless specifically stated in the user documentation The system complies with the requirements for electromagnetic compliance EMC in Australia and New Zealand C The system complies with applicable European directives Typhoon FLA 7000 User Manual 28 9607 62 AB 17 2 Safety instructions 2 8 Labels on Typhoon FLA 7000 Labels concerning use of hazardous substances Label Meaning the decommissioning of equipment This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning Labels concerning laser light Label Meaning CAUTION Avoid exposure to the laser beam when the lid is open ER TTET 1 9 DyDEMRISCISASB OIRKRUTI RAHAT LORIE ERIT TIER CAUTION CLASS 38 VISIBLE AND INVISIBLE LASER RADIATION WHEN OPEN AND INTERLOCKS FEATED AVOID EXPOSURE TO THE BEAM ATTENTION RAYONNEMENT LASER VISIBLE ET INVISIBLE DE CLASSE 3B A LOUVERTURE ET QUAND LE VERROUILLAGE EST DEJOUE EVITER L EXPO
52. onformity is available on request Directive Title 2006 42 EC Machinery Directive MD 2006 95 EC Low Voltage Directive LVD 2004 108 EC ElectroMagnetic Compatibility EMC Directive CE Marking The CE marking and the corresponding Declaration of Conformity is valid for the instrument when it IS e usedasastand aloneunit or Typhoon FLA 7000 User Manual 28 9607 62 AB 1 Introduction 1 2 Regulatory information e connected to other CE marked GE Healthcare instruments or e connected to other products recommended or described in the user documentation and e used in the same state as it was delivered from GE Healthcare except for alterations described in the user documentation Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This Class A digital apparatus complies with Canadian ICES 003 Cet appareil num
53. phor screen 2 Fluorescence button click to read a fluorescent sample 3 Digitization button click to perform digitization 4 Method button click to register or erase a combination of laser and filter 5 Filter Module button click to change or register a filter 6 Preferences button click to set the image file format scan mode correction mode and to enable or disable the ND filter 7 Filter displays the loaded filters 8 Laser displays the status of the loaded laser units 10 Typhoon FLA 7000 User Manual 28 9607 62 AB 1 Introduction 1 4 Typhoon FLA 7000 Control Software Overview of the reader settings window 10 J Typhoon FLA 7000 E ioj x File Edit Help 1 _ a File Save Condition Load Condition Image Folder C Program Files GE HealthcareNT yphoon FLA 7000 Data Browse 2 File Name test reading 11 _ PE EEDIIEE eS 7 3 Reader Settings Fluorescence E DE os E Pixel Size Latitude evs EI c 25 um e L4 8 4 EB smes e 50 um c 400 um a put 500 500 1000 C 200 um 5 Sampling Area Stage Fluorescence Stage k A B C DIE F G H 1 J K LIMINIolD 12 File Size about 10 30 MB Reading Time about 1m45s File Format Gel 13 e Top ND Filter Off EB Start Scan 14 E Scan Mode Standard 15 Part Description al Image folder specify where to save the file after the reading 2 File Name enter the name of a file to save image data
54. phs using storage phosphor screens e Four lasers and multiple filters enable the use of a variety of fluorescent dyes and detection methods e Data is aquired through high resolution sensitivity and wide range linearity e Compatibility with various types of samples for example fluorescent labels fluorescent dyes CBB stain silver stain X ray film and storage phosphor screens 1 4 Typhoon FLA 7000 Control Software System requirements Parameter Minimum requirement Operating system Windows XP Professional SP2 or later or Windows Vista Business SP 1 or later Internal memory 1GB Processor Intel Core 2 Duo processor Hard drive 80 GB Monitor resolution 1280 x 1024 pixels Other requirements One USB 2 0 port DVD ROM drive Typhoon FLA 7000 User Manual 28 9607 62 AB 1 Introduction 1 4 Typhoon FLA 7000 Control Software Overview of the main window The Typhoon FLA 7000 Control Software is used to control use and supervise the Typhoon FLA 7000 4 Typhoon FLA 7000 DI x File Edit Help Mode Settings ana ro i Pb i 2 Fluorescence 3 Typhoon FLA 7000 46 imagination at work 2009 GENERAL ELECTRIC COMPANY ALL RIGHTS RESERVED 4 EE Settings Status Ready Method 5 Y Fiter Module sa on 532nm OK OK e Preferences 6 7 8 1 Phosphorimaging button click to read a storage phos
55. placing filters Step Action 2 Place the new filter in the filter module Push the new filter in until it locks in place Place the filter module in the scanner Step Action 1 Open the filter change door 54 Typhoon FLA 7000 User Manual 28 9607 62 AB 8 Filter module settings 8 4 Installing and replacing filters Step Action 2 Insert the filter module in the filter module holder 3 Close the filter change door Register the filter change in Typhoon FLA 7000 Control Software Step Action al Click the Filter Module button in the main window T Fitter Module 2 Click the desired filter position on the filter tray 0580 4 R670 3 7520 Filter Module Module 1 ij oP 2 2 Y520 Load Fiter Module Typhoon FLA 7000 User Manual 28 9607 62 AB 55 8 Filter module settings 8 4 Installing and replacing filters Step Action 3 Select the filter to be registered from Filter List Filter Module Settings L Filter List 10580 4 R670 lt 3 7520 2 P 41 Filter Module Module1 1 01 2 Y520 3 R670 Load Filter Module 4 oO 0580 Cancel 5 OK 4 Click the Insert button to register the filter ee TIP You can also drag the filter from Filter List to the desired filter position 5 Click the OK button 56 Typhoon FLA 7000 User Manual 28 9607 62 AB 9 Operation 9 Operation 9 1 Operation overview
56. rator Typhoon FLA 7000 User Manual 28 9607 62 AB 2 Safety instructions 2 6 Radiation hazard prevention 2 6 Radiation hazard prevention Controlled area Paragraph 1 of Article 1 of the Law Enforcement Rules for Prevention of Radiation Hazards due to Radioisotope and so forth Prime Minister s Office ordinance No 56 defines the controlled area as a place where the dose equivalent related to external radiation exceeds the dose equivalent determined by the Director General of the Science and Technology Agency hereinafter referred to as the Director General the concentration of radioisotope in the air exceeds the concentration determined by the Director General or the radioisotope density on the surface polluted by radioisotope exceeds the density determined by the Director General Limit of superficial pollution Paragraph 3 of Article 4 of Notice No 15 of the Science and Technology Agency that determines the quantity etc of radiating isotope specifies that the density of radioisotope on the surface polluted by radioisotope must be one tenth of the density defined in Article 8 Article 8 and Table 3 of this Notice define the limits as shown below 1 Superficial density of radioisotope that radiates alpha rays 4 Bq cm 2 Superficial density of radioisotope that does not radiate alpha rays 40 Bq cm Installation site of instrument This instrument is capable of reading not only Storage phosphor screens but also fluor
57. re is read as digital image information at the maximum resolution of 25 um per pixel 40 pixels mm and recorded in the analyzer unit e Erasure You may reuse a general purpose Storage phosphor screen by erasing an after image 11 2 Compatible storage phosphor screens Storage phosphor screens with magnetic layers for sticking on the phosphor stage are usable 11 3 Handling precautions Check the exposure environment NOTICE gt Avoid exposing storage phosphor screens in places where the environmental radiation may be increased for example rooms with concrete walls or in basements Do not stack cassettes during exposure 76 Typhoon FLA 7000 User Manual 28 9607 62 AB 11 About storage phosphor screens 11 3 Handling precautions Wear gloves CAUTION Always wear cotton gloves when handling storage phosphor screens Use a suction rod when taking the storage phosphor screen out of the cassette Prying the storage phosphor out may result in the edge peeling off making the storage phosphor screen unusable Handle the storage phosphor screen carefully e Do not scratch or bend the storage phosphor screen e Keep the storage phosphor screen free from dust Protect the storage phosphor screen from water and volatile solvents e Dry the sample thoroughly before exposing it e Wrap wet samples in plastic film and make sure that no liquid exits Use double layers if the sample contains volatile solv
58. s missing Failed to open End User License Agreement Please Countermeasure Install software for note that a PDF reader e g Adobe Reader is needed Viewing PDF documents Failed to open Getting Started Please note that a PDF reader e g Adobe Reader is needed The disk capacity is insufficient The available disk space is insufficient Countermeasure Free up disk space on the computer Countermeasure Store the data on a dif ferent disk No disk space See above Errors and warnings in the main window Error message Meaning and countermeasure Cannot detect Typhoon FLA 7000 Please The scanner is not detected check connection and power Countermeasure Check that the scanner is turned on and connected to the computer Typhoon FLA 7000 User Manual 28 9607 62 AB 83 13 Troubleshooting Errors and warnings in the Filter Module Settings Error message Meaning and countermeasure window Filter module has not been set up Click the filter module button to set up the filter module The filter module is not installed or properly configured Countermeasure Install the filter module See Sec tion 8 4 Installing and replacing filters on page 52 Please enter a filter name The filter has no name Countermeasure Type a name in the Name field The filter name has already been used Please change the name The name of the filter is being used for another filter Co
59. save the current reading conditions in a file if desired For details refer to the User Manual 11 Load Condition click this button to load previously saved reading conditions if desired For details refer to the User Manual 12 File Size the estimated size of the result data file is presented 13 Reading Time the estimated time required for the scan is presented 14 Top return to the main window 15 Start Scan start the scan The sample must be loaded before starting a scan 12 Typhoon FLA 7000 User Manual 28 9607 62 AB 2 Safety instructions 2 Safety instructions 2 1 Introduction The Typhoon FLA 7000 is powered by mains voltage and is used to image samples that may be hazardous Before installing operating or maintaining the equipment you must be aware of the hazards described in the user documentation Follow the instructions provided to avoid personal injury or damage to the equipment 2 2 General precautions WARNING Do not use the equipment if smoke strange noises or strange odors can be perceived or if the equipment becomes unusually hot This may result in fire or electric shock Stop using immediately turn off the power switch and unplug the equipment from the power outlet Contact your local GE Healthcare representative to request repair WARNING Do not damage the power supply cord by bending twisting heating or allowing them to become pinned u
60. services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9607 62 AB 12 2009
61. sonnel Suction rod included The suction rod is used to lift up or place the the storage phosphor screen on the phosphor stage Press the suction rod against the storage phosphor screen Place a finger on the end of the suction rod then lift up the storage phosphor screen Release the finger to release the storage phosphor screen Suction rod Typhoon FLA 7000 User Manual 28 9607 62 AB 31 3 System configuration 3 3 Accessories Titer plate TP plugin included The TP plugin is used when reading titer plate samples USB cable included The USB cable is used to connect Typhoon FLA 7000 to a computer 32 Typhoon FLA 7000 User Manual 28 9607 62 AB 4 Installing and moving Typhoon FLA 7000 4 Installing and moving Typhoon FLA 7000 CAUTION Only authorized service personnel is allowed to install Tyohoon FLA 7000 Contact your local GE Healthcare representative for help and advice 4 1 Site requirements Note The Typhoon FLA 7000 is intended for indoor use only Power supply 100 to 240 V AC 3 0 to 1 5 A Line frequency 50 to 60 Hz Placement Stable horizontal surface out of direct sunlight Ambient temperature 10 to 30 C Humidity 20 to 75 non condensing Maximum altitude 2000 m above sea level Initial delivery inspection Upon receiving Typhoon FLA 7000 e inspect the package for external damages e check that all items in the packaging list are included
62. structions 2 8 Labels on Typhoon FLA 7000 Labels at rear of Typhoon FLA 7000 GE Healthcare Bio Sciences AB den FFLUORESCENFIMAGEANAISZERIT FLUORESCENT IMAGE ANALYZER IMAGE ANALYZER Model Typhoon FLA 7000 MRO Tuphopp LAZ90 til asa SEE Nea Suely Vaage 100 240 V MANUFACTURED c UL us Mox Curent 30 158 LISTED Protection Closs 1P20 FETT toons cau Manufacturing Year Complies with FDA performance standards for laser products except A C xX for deviations pursuant to Laser m nios Notice No 50 dated June 24 2007 x FUJIFILM Corporation RO Teste AAN MADE IN JAPAN 4051101498 Ths device comples with part 15 of the FOC Rules Operation is subject to the folowing two conditions 1 this device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation This Gass A digital apparatus meets al requirements of the Canadian Interference Causing Equipment Regulations Cat apparel numerique de la classe A respecte toutes les exigences du Reglement sur le maternel boullleur du Canada IX ETEN 93238B DEA RUFE ALEI E LOMSCEMI TRA CAUTION CLASS 38 VISIBLE AND INVISIBLE LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM ATTENTION RAYONNEMENT LASER VISIBLE ET INVISIBLE DE CLASSE 3B A L OUVERTURE EVITER L EXPOSITION AU RAYON 20 Typhoon FLA 7000 User Manual 28 9607 62 AB L
63. ton 5 Select destination folder in the dialog olita DE e Click the Next button to install the software at the default folder C Program Files e Click the Change button to install to a different folder 6 Click the Install button Typhoon FLA 7000 User Manual 28 9607 62 AB 43 5 Installing Typhoon FLA 7000 Control Software 5 3 Install Typhoon FLA 7000 Control Software under Windows Vista Step Action 7 If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified program wants access to your computer Click Allow 8 Click the Finish button The installation of Typhoon FLA 7000 Control Software is now completed 44 Typhoon FLA 7000 User Manual 28 9607 62 AB 6 Uninstalling and upgrading Typhoon FLA 7000 Control Software 6 Uninstalling and upgrading Typhoon FLA 7000 Control Software Before you begin Log in using a Windows account with administrator privileges Uninstalling Typhoon FLA 7000 Control Software under Windows XP Step Action il Open the control panel and select Add or Remove Programs 2 Select Typhoon FLA 7000 and click the Remove button PI aie E erty reeked perqem ci nites FA sainhs atos Dim al ster Hal SET Franaa 3 5 SPL 1325H EE AAI clue Ha aa SET Fansat 3 5 SPL 3350596 Mot c JPartar Keane th Erer rame L Private Ma age Lar H ula LT ad glucose rure adeanzece vebdaton cel FUJII AS Santier Or mta neces tenet Frere e 5a 24EB GB
64. untermeasure Choose another name for this filter or change the name for the filter already having this name The maximum number of the filters you can register is 50 Please delete an unnec essary filter before registeringa a new filter No more filters can be stored in the software Countermeasure Delete a filter before storing a new one Errors and warnings in the Method Settings window Error message Meaning and countermeasure Please input a method name No name has been assigned to the method Countermeasure Type a method name in the Name field The maximum number of the The maximum number of methods are stored No more methods is 50 methods can be registered Countermeasure Use an existing method or delete a method before registering a new one This method name has been al The assigned name is being used by a different method ready used Please change the Countermeasure Type a different name in the Name field name 84 Typhoon FLA 7000 User Manual 28 9607 62 AB 13 Troubleshooting Errors and warnings in the Reader Settings window Error message Meaning and countermeasure Failed to retrieve image data from the scanner Check the scanner connection There was a problem transferring data to the computer Countermeasure Check the scanner connection to the computer Cannot detect Typhoon FLA 7000 Please check connection and pow er The scanner was not dete
65. ype a name for the filter combination in the File name field 4 Click the OK button 5 Click the OK button to return to the main window 8 3 Loading a filter combination Step Action 1 Click the Filter Module button in the main window UY Fitter Module 2 Click the Load Filter Module button 3 Select a filter module to load in the Load Filter Module dialog box 4 Click the OK button 5 Click the OK button to return to the main window CAUTION When removing the filter module make sure to click the Filter Module button and remove the filter module after the window changes to the Filter Settings window If the filter module is forcefully removed the area where the filter comes in contact with the PMTs may be damaged 8 4 Installing and replacing filters Remove the filter module from the scanner Step Action 1 Click the Filter Module button in the main window UY Fitter Module A status message appears Wait until the message is closed before proceeding to the next step 52 Typhoon FLA 7000 User Manual 28 9607 62 AB 8 Filter module settings 8 4 Installing and replacing filters Step Action 2 Open the filter change door Remove and replace filters in the filter module Step Action 1 Press the notch on the bottom of the filter module and pull out the filter Typhoon FLA 7000 User Manual 28 9607 62 AB 53 8 Filter module settings 8 4 Installing and re

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