Microliter Flow M.P.C User`s Manual
Contents
1. Position2. This pump does not include stall detection Serial commands and their meanings Commands KEY Return to keyboard control Exit remote mode RUN Start infuse forward direction STP Stop motor CLV Clears volume accumulator to zero CLT Clears target volume to zero dispense disabled REV Reverses pumping direction Commands with numbers MMD number Set syringe diameter units are mm Rate is set to zero after MMD command ULM number Set flow rate and range units are microliters per minute MLM number Set flow rate and range units are milliliters per minute ULH number Set flow rate and range units are microliters per hour MLH number Set flow rate and range units are milliliters per hour MLT number Set target infusion volume units are ml or ul depending on range Numbers can be between 0 and 1999 Leading zeros and trailing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number will be rounded Publication 5415 003 REV B 4 ul Modular Pumping Component Harvard Apparatus Appendix D Serial Communication Queries DIA TAR Returns diameter value units in mm Returns rate value set in current range units Returns current accumulated infused volume units in ml or ul depending on range Returns model and version number of firmware Returns target volume units in ml or pl depending on range value format nnnn nnn The returned value is an 8 chara
3. Character deiay fE milliseconds F Appendline teads to incoming ine ends I Eorce coming data to Mat ASCH F Wrap tines thet exceed terminal vadth Ce sm You may want to save the setup information under a descriptive filename 4 At the PC launch byperterminal with the above setup specifications if it is not already running Type VER at the PC keyboard and verify that the pump module s version is displayed at the PC terminal 5 Type RUN to start the pump type STP to stop the pump After starting the pump 0 gt should be displayed indicating address 0 pump is infusing After stopping the pump 0 should be displayed Publication 5415 003 REV B p ul Modular Pumping Component Harvard Apparatus Appendix A Type Syringe Size for OEM ul Pump with Holder For 1 Syringe Flow Rate Range Flow rate Min Max Linear Force Display Keypad Interface Limit Switch Drive Step Rate Pusher Travel Rate Connectors Power Size H x W x D Mounting Dimmensions Weight Enviromental OEM pl Module Specifications Microprocessor single syringe infusion withdraw Glass or Plastic From 0 5 uL min to 1 mL max 0 0014 ul hr with 0 5 ul syringe 854 ml min with 1 ml syringe 7 Ib max None None RS 232 One for each direction end of travel Motor 0 9 step angle motor Control L R Drive 0 75A per phase 1 4 stepping Drive Ratio 1 1 Direct Drive Lead sc
4. DC power cable extension cable 1143 209 e 1 6 ft Motor limit switch extension cable 1143 211 e 1 RS 232 cable 70 2022 e 1 User documentation e 1 6 oz jar of grease e 1 Syringe pump unit Publication 5415 003 REV B ul Modular Pumping Component Harvard Apparatus Installation Location Requirements for the Syringe Pump A sturdy level clean and dry surface EE e Minimum of one inch 2cm clearance around the pump e Appopriate environmental 8 750 Recommended 8 37 fan 22 23 Cabinet Cutout 21 3 conditions e A well ventilated room A Y Mount into an appropriately Hh 237 t rated enclosure or cabinet N 6 0 Dimensions inches cm Mounting Holes for 8 Screw Loading the Syringe 1 Fo CON IRS SIRS Release the Syringe pusher block 4 by pressing the bronze button 3 on the side of the pusher While holding the bronze button in slide the pusher to the right Raise the spring loaded syringe retainer 1 and swing it out of the way Lay the loaded syringe in the V shaped holder Swing the retainer so it holds the syringe in place Move the pusher so it makes contact with the syringe plunger Adjust pusher block Thumbscrews amp bracket 2 until the syringe plunger is completely captured The limit switches can be easily adjusted by loosening the 2 nuts not shown sliding the switch to desired position and retighte
5. approved power supply Be sure to observe proper polarity of con nections when hooking up DC supply voltage CAUTION FAILURETO MAINTAIN POLARITY MAY RESULT IN DAMAGE TO THE UNIT AND WILL VOID THE WARRANTY 12 Vdc return PINS 1 amp 2 of connector J7 12 Vdc positive PINS 3 amp 4 of connector J7 see input connections diagram page 6 Ground the Product This product is grounded through a ground stud located on the under side of the base plate To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or output terminals of the prod uct ensure that the product is properly grounded Make Proper Connections Make sure all connections are made properly and securely Any signal wire connec tions to the unit must be no longer than 3 meters Avoid Contact with Heatsinks Some components normally operate at temperatures exceeding 70 deg C To avoid injury do not touch any heatsinks Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections Avoid Exposed Circuitry Do not touch any electronic circuitry Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection Avoid Liquid Spills The Microliter Pump has openings in the baseplate to accomodate limit switches Care should be taken to avoid spills that could allo
6. issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replace ment and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as CAUTION completely as possible preferably using our part EOR RESEARCH USE ONLY numbers If practical enclose a sample or drawing We offer a complete reconditioning service NOT FOR CLINICAL USE ON PATIENTS CAUTION This pump is not registered with the FDA and is not for clinical use on human or veterinary patients It is intended for research use only Publication 5415 003 REV B ul Modular Pumping Component Harvard Apparatus General Safety Summary Please read the following safety precautions to ensure proper use of your modular syringe pump To avoid potential hazards and product damage use this product only as instructed in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired To Prevent Hazard or Injury Use Appropriate Power Supply Use only an SELV
7. the unit Serial numbers are located on the underside of the baseplate Calibration All syringe pumps are designed and manufactured to meet their performance spec ifications at all rated voltages and frequencies Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCI DENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclu sion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact your local distributor or Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 valid only in the U S outside U S call 508 893 8999 Goods will not be accepted for return unless an RMA returned materials authorization number has been
8. 6 4 61 35 00 ml min min max jomin max min max min max 0 0014 0 0031 0 0061 0 0149 0 0289 0 0728 0 1448 0 2909 0 7218 1 451 2 900 167 2 22 35 50 29 98 58 243 4 473 0 1 191 2 371 4 765 9 999 9 999 9 999 9 999 0 0001 0 0001 0 0002 0 0003 0 0005 0 0013 0 0025 0 0049 0 0121 0 0242 0 0484 2 786 0 3725 0 8383 1 6430 4 057 7 883 19 85 39 52 79 41 197 0 395 7 791 8 9 999 0 0001 0 0001 0 0001 0 0001 0 0001 0 0001 0 0002 0 0003 0 0008 0 0015 0 0029 0 1672 0 0223 0 0502 0 0985 0 2434 0 4730 1 191 2 371 4 765 11 82 23 75 47 50 2 738 0 0001 0 0001 0 0001 0 0001 0 0001 0 0001 0 0001 0 0001 0 0001 0 0001 0 0001 0 0028 0 0003 0 0008 0 0016 0 0040 0 0078 0 0198 0 0395 0 0794 0 1970 0 3959 0 7918 45 64 ul Modular Pumping Component Harvard Apparatus Appendix D Serial Communication Commands Queries and Responses After each transmission to the pump terminating with a CR character ASCII 13 the pump enters remote mode and responds with the three character sequence CR LF prompt The prompt character indicates the status of the pump as follows prompt meaning ASCII code When stopped ASCII 58 decimal gt When running forward ASCII 62 decimal u When stalled ASCII 42 decimal
9. Microliter Flow M P C Modular Pumping Component User s Manual MA1 70 2204 HARVARD APPARATUS Publication 5415 003 REV B WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with a
10. cter string with leading zeros converted to SP characters ACSII 32 decimal The fifth character is a decimal point ASCII 46 decimal Queries with string response RNG Returns range message character string either ML H ML M UL H UL M Error responses CR LF CR LF prompt Unrecognized command CR LF OOR CR LF prompt Entered value in out of range Publication 5415 003 REV B M Q Modular Pumping Component n ul Harvard Apparatus Appendix E Single RS 232 RXD TXD GND GND IN Host Computer Connection Appendix F Dual RS 232 optional RXD TXD GND GND IN Host Computer Connection Publication 5415 003 REV B GND GND RXD TXD OUT Chain Connection to Additional Pump s Declaration of Conformity Application of Council Directive s 73 23 EEC Standard s to which conformity is declared EN61010 1 1993 A2 1995 Manufacturer s Name Harvard Apparatus Inc Manufacturer s Address 84 October Hill Road Holliston Massachusetts 01746 U S A Type of Equipment Syringe Pump Model No 70 2204 Microliter Pump Module I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place United States of America Date September 06 2002 C Signature Beth Bauman HARVARD Treng APPARAT US VP Engineering Operations
11. ning the nuts into place I mm FJ 7 Publication 5415 003 REV B ul Modular Pumping Component Harvard Apparatus Operation Getting started Plug one end of the 6 ft power cable supplied into J7 connection on board Then plug the other end of the power cable into your power supply not included Be sure to maintain proper polarity of connection so as not to dam age the unit Plug the RS 232 cable supplied into J4 connection and your PC Note Refer to page 6 of this manual to reference the board connections Working with the pump The safest way to use the ul Modular Pumping Component is in the volume dispense mode The pump will automatically stop when target volume is dis pensed Check Syringe Often Limit switches are incorporated onto the pl Modular Pumping Component to stop the pump at the end of limit travel for infusion and withdraw Be sure to adjust and set these switches properly to prevent damage to small fragile syringes Maintenance Keep the ul Modular Pumping Component clean and dry Avoid liquid spills that may find their way into the electronics A small container of grease is provided for periodic lubrication of the lead screw It is important to keep the leadscrew clean and lubricated To clean the exterior surfaces above the baseplate use a lint free cloth to remove loose dust For more efficient cleaning use a soft cloth dampened with water or an aqueous solution of 75 isopro
12. ny changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required Modular Pumping Component n ul Harvard Apparatus Table of Contents SUBJECT PAGE NO General Information Warranty and Repairs 2 General Safety Summary mrnnnnnernnvvnnnnnnnnnnnnnnnnn 3 4 Introduction Theory of Operation maraspdssnpejedend 5 FO AOS sesiesnevaninavle cieeviasindesdsuteretssvensidanseencnsstnsss 5 ul Modular Pumping Component Input Connections What s Included 3 4scbecotossansiuaredaxedeetysiuradevetperetances 6 Installation Location Requirments LuussatkkaadkendainG 7 Loading the Syringe vunnet beunnne 7 Operation Getting Started Working with the Pump errrnnnnnvvrrovrrrrrvereversernn 8 Check SVG Cia sv saintoniaintnrevavenia cumini ieee 8 Nene 8 Protecting Small Fragile Syringes rrrrrrrrrrrrnn 8 Hyper Terminal nn ernrnnnnnnvevevnnnnnnvevevnnnnnnevevennnnnr 9 10 Appendices A SPOCHICALOMS unde bakende nitet udi 11 B Table of Popular Syringe Diameters 12 C Flow RBs ae 13 D Serial Communications Commands Queries and Responses 14 15 E Serial Port Connections Single RS 232 16 F Serial Port Connections Dual RS 232 16 Publication 5415 003 REV B ul Modular Pumping Component Harvard Apparatus General Information Serial Number All inquires concerning our product should refer to the serial number of
13. pyl alcohol Do not allow fluid to pass through the holes in the baseplate and get onto the electronics If the pump does not work properly contact Harvard Apparatus for appropri ate instructions Publication 5415 003 REV B 5 pl Modular Pumping Component Harvard Apparatus HYPER TERMINAL Terminal Emulation Software Remote Control Via The RS 232 Interface Using HYPERTERMINAL Normally included with most Windows operating systems ul Modular Pumping Component 1 Connect tbe RS 232 cable supplied between the ul Modular Pumping Component RS 232 IN port and a PC s serial port 2 On the PC running a Microsoft Windows Operating System select START PROGRAMS ACCESSORIES HYPERTERMINAL HYPERTERMINAL to start the Hyperterminal application If Hyperterminal is not available install it from the Microsoft Windows Operating System Install disks or CD ROM 3 Set up the appropriate COMPORT for the following Erte the sea code witout he bag d stoso prest agenter fl Bhana Connect using foer Configure ASCII Setup Baud Rate 9600 Peho ped characters locally Data Bits 8 Line delay 0 Parity None Character delay 0 Stop Bits 2 Wrap lines Flow Cntrl None Emulation Auto Detect Publication 5415 003 REV B pl Modular Pumping Component Harvard Apparatus HYPER TERMINAL Terminal Emulation Software I Send tne ends with line teeds E Echo ped cheracters loceily Une delsy fl milisecords
14. rew pitch 48 threads per inch 1 4 48 6 8 sec step 416 7 ul step 2 9 um min 47 6 mm min Min Max RS 232 RJ 11 telephone jack DC Power 4 pin Header Friction lock 12VDC 5 1A 12W Note Use only a SELV approved power supply 9 25 x 3 25 x 4 50 Lx W x H 23 5cm x 8 3cm x 11 4cm 8 75 x 2 75 Mounting holes for 4 8 screws 22 2cm x 7 0cm 1 85 Ibs 0 84 kg Operating Temp 0 to 35 deg C natural convection cooling Storage Temp 20 to 70 deg C Humidity 20 to 80 RH non condensing Note operating temperature may be extended with forced air cooling Publication 5415 003 REV B gt ul Modular Pumping Component Harvard Apparatus Appendix B Syringe Inside Diameter Unimetrics Series 4000 amp 5000 Size Diameter 10 ul 0 460 mm 25 0 729 50 1 031 100 1 460 250 2 300 500 3 260 1000 4 610 SGE Scientific Glass Engineering Size Diameter 25 ul 0 73 mm 50 1 03 100 1 46 250 2 30 500 3 26 1000 4 610 Hamilton Microliter Series Gastight Size Diameter 5 ul 0 103 mm 1 0 1457 2 0 206 5 0 3257 10 0 460 25 0 729 50 1 031 100 1 46 250 2 3 500 3 26 1000 4 610 Publication 5415 003 REV B ul Modular Pumping Component Harvard Apparatus Appendix C Flow Rates nominal syringe size Min Tul 2ul 5 ul 10 ul 25 pl 50 ul 100 ul 250 pl 1 000 pl 1ml Publication 5415 003 REV B 0 10 0 15 0 21 0 33 0 46 0 73 1 03 1 46 2 30 3 2
15. rical mechanical enclosure or cabinet The board may be removed for remote operation Features Two Modes of Operation Constant Flow Rate and Volume Dispense The pl Modular Pumping Component will operate continuously in RATE mode or accurately dispense a specific amount of fluid in VOLUME mode When start ing the pump RATE mode will be the default mode To operate in VOLUME mode set a target volume and the pump will change modes to suit desired opera tion Smooth Flow New micro stepping pump profiles deliver very smooth and consistent flow Nonvolatile Memory The pump remembers it s last syringe size flow rate used and configuration settings in it s non volatile memory CE Mark Approved The pl Modular Pump meets all relevant european safety requirements for labo ratory equipment when used in accordance with the guidelines in this manual Publication 5415 003 REV B ul Modular Pumping Component Harvard Apparatus ul Modular Pump Input Connections U7 POWER INPUT CAUTION CONNECTION HOT SURFACES UNE E TANE 8 RUN LED J1 MOTORS CONNECTION CONNECTION OPTIONAL CAUTION HOT SURFACES N J2 KEYPAD CONNECTION OPTIONAL Je LIMIT SWITCH CONNECTION J4 RJ11 RJ12 CONNECTION Defaults Settings Dia 4 61mm 1ml Rate 1 0ml hr Baud 9600 Address 00 Mode Pump What s included Packaged with the pl Modular Pumping Component 1 6 ft
16. w liquid to get onto the circuit board Publication 5415 003 REV B ul Modular Pumping Component Harvard Apparatus General Safety Summary Place Product in Proper Environment Enviromental Conditions Indoor use only Temperature 4 C to 40 C 40 F to 104 F Humidity 20 to 80 RH Well Ventilated Room Altitude up to 2000 m Voltage Fluctuation not to Exceed 10 of Nominal Transient Overvoltage Category II Pump is Rated Pollution Degree 2 in Accordance with IEC 664 Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Protective Ground Refer to Manual Terminal Caution Pinch Hot Hazard Surface Caution ESD Sensitive Device Publication 5415 003 REV B i ul Modular Pumping Component Harvard Apparatus Introduction Theory of Operation The new ul Modular Pumping Component is a low cost highly precise single syringe infuse withdraw pump module capable of low to moderate back pressures The module includes a mechanism hardware and software and requires the addi tion of a power supply and user interface The pump comes in one version and will hold one syringe of any make from 0 5 pl to Iml The diameter of the syringe s is entered via the RS 232 serial port and the internal microprocessor drives a precision stepper motor to produce accurate fluid flow This unit is designed to operate inside an appropriately rated fire elec t
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