2-Needle Array Electrode User`s Manual
Contents
1. to thia product or any products connected to it To avoid potential hazard use thie product only ae specified Ony qualed BTX personnel shouid perform servion procedures To Prevent Hazard or Inju ARCING CAN OCCUR AT HIGH VOLTAGES An untavorabie combination ot parameters such as high vonage senings and smal sample volume with a highly conductive medium might lesd to fashover between the electrodes IARC andor explosive evaporation of the medium Reduce voltage or pulse length to avoid repeating this condition DO NOT OPERATE WITH SUSPECTED FAILURES M you suspect there iz damage to the produ have It inspected by qalted BTX service personne Do NOT CONTACT ELECTRODES To avoid fre ar shock hazard observ all ratings and markings on the product or in this manual before ving the devion AVOID EXPOSURE TO CONTACT Do not inser fingers or uy to remova electrode or ample during pulsing sequence WEAR PROPER EYE PROTECTION DURING ELECTROPORATION DO NOT OPERATE IN AN EXPLOSIVE ENVIRONMENT DO NOT OPERATE IN WET DAMP CONDITIONS Safety Terms and Symbols Terma that appear in this manat j WARNING Warning statement ity Condon or procs tnat cold resultin ATION Caution statements nity A condone or pacts that cold renin nage 1o theae products or ether propery Symbols that may appear on the products A A Danger Functional High Retera Earth Ground Votage Mamusi Terminal Terminal munes 800 272. 10 aore Intended Use Indoor ue oniy Relative Humiiny 2010 80 Maximum Atitude 2 000 m 8562 Pollaion Degree n Insulation Category cart Physical Characters Mods sos says Needle Spacing 10mm Emm Needle Length 20mm 20mm Needle Material Stainless Stal Stainless Stat Handie Length Bem Bem CMe 620 830 and 2001 The Enhancer 20008 Monitoring System E Depending an butler composition and generator capability APPENDIX B REPLACEMENT PARTS Order No Model Description Needle Array Ki 10mm asoa sao Needle Array Handie 10 mm gap snw so 2 eee Aray Tp Ym oap wne sar Now Array Ki 5 mm Array Handle 5 mm gap snn s Noel Array Tip 5 mm gap 2 145 0050 ENHANCERQOO0SC The Enhancer 3000 Monitoring APPENDIX C TROUBLESHOOTING Piense contact BTX Technical Service at any of the numbers isted below in the event ot any fale A Division of Harvard Apparatus a4 October iN Road olson MA 01746 1508 USA ol Free 1 800 272 2775 US only Phone 506 99 2008 tchaupport bgharvardeppartu com internet vee buco com eck on customer service The Enhancer 3000 Monitoring System ov Shown wan communications module not installed CAUTION For RESEARCH USE ONLY NOT FOR CLINICAL USE ON PATIENTS mnes 800 272 2775 ica 508 893 8999 www btxonline com EU Directives WEEE and RoHS To Our Valued Customers We are commited to being a good exports chizen As part of th
3. 2 2775 ica 508 893 8999 www btxonline com 2 Needle Array Electrode OPERATION GETTING STARTED WARNING HAH VOLTAGE Maka sure the BTX alactropoato is switched Grasping the 2 Needle Array Handle poston the handle vera 2 Neadle Array Tip and push to secure the array to the handie 2 Attach the handle to a BTX electroporator via the high volage banana cables Following instructions for the electroporstor set the appropria parameters on the generate Set up The Enhancer 3000 Monitoring System st this time Hin use Prepare thous and sample for electroporation Appiy samplato tenue ust betore electroporation 5 Remove the safety shield protcting the need pce Into tna tatu and deliver the electroporation pulse 6 Discard the Zed array tip n a typical biohazard sharps container Rater to your county s waste management organization for proper disposal practican 7 Clean the 2 Needle Array Handle with a sott loth or teue ecensary moisten the cloth or tassua with a ute detergent solution Note The 2 Nove Array Tip are designad tor single use resting hoe elacvades sterilize wah ethanal or thylene oxide DO NOT autoclave Maximum usage is three sets of pubes APPENDIX A SPECIFICATIONS 2 Needie Array Electrode Electrical amp Technical Specifications Standard Capabitties Votage Range ot 500v0e Consent 1 usec to 9 mase Pulse Number Range 11w98 Operating Temperature S8
4. 2 Needle Array Electrode The Needle Array Electrode is a needle style slacradeepecifelly designed tor in vivo gene delivery in muscle The electrode consiste fa reusable 2 Needie Array Handie and stele disposable two ed ray tips Tha needle array tips are convenientiy packaged in Six Pacts allowing for guaranteed seit and designed oft conveniently in a sarile hood The neds temsahos are made of medial grade stainless steel To secure the needle array tp to the Nedle Array handie simply position the handie over a ned sray Up and push to secure onto the handle Atach the handie to s BTX slectropoator vis the high volage banana cables Remove the sed zalety shield place into the issue and delve the pulse Discard the needle array tip and peepar for the next experiment Tha Z Needle Array comes in B mm and 10 mm electrode spacing US Patent 05 279 525 US Patent 05 702 350 HARVARD APPARATUS The Electroporation Experts Order No Modei Dosoription 5017 Beedle Array 10 mm kit asaz 5502 Neal Array Handle 10 mm gap asno ss 2Needia Array Tip 10 mm gap pkg of 5018 8 mm iit aoz saa 2Needie Array Handle 5 mm g asaz 552 2 Neda Array Tip 5 mm gap pkg ot 5 mines 800 272 2775 ica 508 893 8999 www btxonline com 2 Needle Array Electrode GENERAL INFORMATION Warranty TxMarverd Apparatus warranties this BTX2 New Array Electrode for a period ot 30 days from date of purchase At iis opti
5. at commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recyciing Waste Electrical snd Electronic Equipment WEEE and te second liming the use of certain substances Raswietion fn the uae of Hazardous Substances RoHS Over tima tese Directives wil be implemented in the nations lawe of esoh EU Member Stat Once the final national regulations have been put into place recycling wil be offered tor our products which are within ne scope of the WEEE Directive Products faling under the scope of tha WEEE Directives available tor sale after August 12 2005 wl be identified with a wheels bin symbol Two Catagories ot products covered by the WEEE Directive are curently exempt trom the RoHS Directive Category amp medal devizas th the exception of implanted or Infected products and Category 8 monitoring snd contrat instruments Most of our produce falta ether Category 8 or 9 and are currently exempt from the RoHS Directive We wili continue to monitor the application ofthe RoHS Directive to hs producte and wil comply with any changes ss they spy WEEE RoHS Compliance Statement Do Not Dispose Product with Municipal Waste Special Collection Disposal Required HARVARD APPARATUS The Fhcropersion Papers 84 October Hill Road fortes 800 272 2778 ica 508 893 8999 ona techsupport btx ha
6. on BTXHarvard Apparatus wil repair or replace the tem It is found to be detective as to workmanship or materi This warranty does not extend to damage routing rom misuse neglect or abuse normal wear and tear or aocident This warranty exiende original customer purchase IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some statas do not Slow exclusion or imitation of incidental or consequentia damages athe above imitation or exsiusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some statea do not allow tnis limitation onan implied warranty s0 the above limitation may not apply to you Is defect aries within the 90 day warranty period promptly contact BTX Harvard Apparatus 8 October Hil Raed Holliston Massachusetts 017461388 using our Us cali 1508 09 80 Goode wil nat be scceped for retum unless an RMA Return Materials Authorization number has been by our customer service department The customer is responsible tor shipping charges Please allow a reasonable period at time for completion of repaire or replacement ana return the Unit is replaced the replacement unit i covered oniy forthe remainder of the original warranty pelo dating trom the purchase tthe originai device This warranty give you specifie ight and You may aleo have other rights which vary from state to state Note BTX electrodes a
7. re not recommended for use with power supplies or cables from other manufacturers Such use ie completely at tne customer ow risk os Imay resultin damage creste unsafe conditions and wil immediately void the 90 day warranty IMPORTANT Raad sl Instructions Warnings and Precautions prior to usa Technical amp Customer Servico BTA is the utimate resource fr technical information on the use igh voltage bacterial transformation and genera electroporation fof molecules and drugs into cell We constantly Wack and monitor scientie publication inthis are Our Technical Service group extracts and enters parent information such a results nd parameters rom these papers into a Protocol database his database is available via the BTX website Please Visit For technical assistance addtional information oF an ingulyrequst for repair service contact BTX Marvard Apparatus Technical SupporiCustomer Service Group at BIXe A Division of Harvard Apparatus 84 October Hill Road Holliston MA 01746 1388 U S A Toll Free 1 800 272 2775 US oniy Phone 1 508 893 8999 Fax 1 508 429 5732 E mail techsupport btx harvardapparatus com Internet www bbxonline com ciek on customer servies ouside the United Stats and Canada al 1 508 890 3990 or Contact your nearest BTX Dietbutor A complete ito diswibutors GENERAL SAFETY INFORMATION Review the following satety precautions to avoid injury and prevent damage
8. rvardapparatus com Publication 5602 009 REV CS Holliston MA 01746 tox 508 429 5732 web WWW btxonline com
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