LCD 5000 User Manual
Contents
1. internal memory HOLD suspends the run of the program END interrupts the run of the program and returns all time depending values to the conditions as they were set by the program at the time 0 00 and this applies even in the case of direct control see H 1 SPECTROPHOTOMETRIC DETECTOR Y AE PROG Q nape ABS 0 123456 1 250nm t 1 005 lt a J gt ar o LCD 5000 V r V J N O1 ABS CYCLE LINE nM HELP INSERT MODE uv LAMP HIGH uv vis LAMP PREAMP LAMP OFF TEST MARI X Low IX 2 NS J RUN Fig 4 Keyboard PROG switches the control mode over to the program editing MODE modification of the configuration of the pump assembly operation 1 1010 and entering of numerical data ENTER keyboard for the confirmation of the number entered possibly for the selection from the menu 3 direction keys for the movement of cursor under individual positions of the display HELP will provide current user s information If you press twice you will call the operating instructions for using the HELP instructions If you are using the HELP com mand the keys ENTER and gt are active The direction keys en2. ea br REO RR 13 AAA control inputs and outputs e cistitis L4 Ro tine Tene MIO eon ue dada d Pede T5 C 42 Inspection of lamps tias pera 5 EA Troubleshooting rica 15 C 43 Problems with the cell ssp rd 15 E large OIE edad iei Pod ep o ie L5 433 Little light AAA A A AA A AAA AA 16 E 434 Discharge lamp replacement A L6 Lud Lamp replacement ES E DESCRIPTION OF FUNCTION arresta aita gae puces Eso rr 19 20 ID Tc 20 E ACCESSORIES AND REPLACEMENT PARTS L Build accessories eicere EO A ERU LEE QE ERI ied 6 4 Other accessories and replacement parts uni n beer e een ee ey 23 TABLE OF CONTENTS costera isa ideas 24 C 8 1 List of picturtes and tables eibi prisas toria ds PS INGOS s r o Page 24 LCD5000 Section 8 TABLE OF CONTENTS 8 1 Fig Fig Fig Fig Fig Fig Fig Fig CONDO BK WH List of picturtes and tables PEI Gu iene ane daddies hee ame ae halos anaes nee 8 AA AI AN A ni ia 8 Example of the chromatography system interconnection 9 Keyboard Ee P ITI TO PME L6 A aiii as quitus b qe pd Location of the lamp and discharge lamp IS Optical chart of the detector us adlata deba dicU ew da
3. discharge lamp in the holder 5 Terminal board used for the connection 3 Discharge lamp holder of the discharge lamp Fig 6 Discharge 8 Mount the discharge lamp holder on the detector holder Be sure not to forget about the flexible washer under nuts otherwise it will not be possible to adjust the discharge lamp 9 Tighten the nuts only partially in such a way that the discharge lamp can be moved 10 Connect the discharge lamp to terminals The red wire must be connected to the terminal identified in a red colour 11 Turn the apparatus on and switch on the discharge lamp 12 Adjust the wavelength at a value of 250nm 13 With the help of the function PREAMP TEST 4 1 5 observe the intensity of the light in the measurement branch CUV 14 Move the discharge lamp upwards and downwards as well as to the sides so that the light intensity can be as large as possible 15 Tighten the nuts for the discharge lamp to be fixed 16 Release the discharge lamp socket so that it will be possible to rotate the discharge cell 17 Rotate the discharge lamp carefully and search for a maximum intensity of the light 18 Tighten the socket again 19 Reassemble the rear cover INGOS s r o Page 17 LCD5000 Section 4 SERVICE 4 3 5 Lamp replacement If you are replacing the lamp observe the following instructions In fig 7 you can see the location and connection of the lamp Caution Hold the lamp alwa
4. 1 258nm t 1 685 Wlen Of fs B Offs B 6496627 Ref ABC 17895 Save service In the place of the ABS value also other data can be displayed CUV REF RAT NUL The display of individual data items shall be switched over by using the key ENTER if cursor is situated on the item ABS The meaning of individual data is as follows Exit Escape from the mode preamp test ABS Absorbency CUV Light by the cell Max 0 95 REF Light by the reference Max 1 5 This data item serves for the control of the lamp and discharge lamp See 4 2 1 RAT Ratio of CUV REF Max 0 95 NUL Zero of the amplifier 1 Wavelength pre set The wavelength can be adjusted within range from 0 to 700 nm For other details see If the adjustment is being carried out the display reads Wrk t Time constant value The time value can be adjusted within a range from 0 04 to 2 50 s INGOS s r o Page 14 LCD5000 Section 4 SERVICE Wlen Offs Offset of the wavelength This constant is adjusted by the manufacturer or servicing technicians during the adjustment of the apparatus Offs Offset of the converter This constant is adjusted by the manufacturer or servicing technicians during the adjustment of the apparatus Ref ADC Value of the auxiliary reference converter It serves for the servicing pur poses Save service Servicing information storage 4 2 Routine maintenance The detector LCD5000 does not r
5. fig 2 Main switch Main fuse T 1 6A Use always a fuse of the same type while making a replacement The mains inlet for the connection of a power supply cord The apparatus features an impulse power supply source which ensures the power supply of 110 to 230 V 50 as well as 60 Hz INGOS s r o Page 7 LCD5000 Section 3 PUTTING INTO OPERATION 1 Photometer body 4 Inlet into the cell 2 Cell 5 Fastening nuts 3 Photometer probe AI Fig 1 Cell POWER T 1 64 AUX OUT 1 Power supply switch 5 Connector RS232 special equipment 2 Power supply fuse 6 Two connectors RS485 3 Power supply inlet 7 Connector of the analogue output de 4 Connector Aux Output signed for the recorder Fig 2 Rear panel The connector AUX OUT for the connection of TTL outputs and inputs Connector RS232 As a standard it is not fitted its fitting is made on an optional basis INGOS s r o Page 8 LCD5000 Section 3 PUTTING INTO OPERATION Two connectors RS485 These connectors are connected in a parallel manner They serve for the computer connection of the detector The connectors are two so that it can be possible to connect more apparatuses to a single communication channel The detector will be connected through a special converter to the computer serial port the converter is a part of the delivery of the computer software A typical example of interconnection of the chromatography
6. for the measurement in the UV range from 190nm to 370nm The discharge lamp is turned on by using the keys UV LAMP HIGH UV LAMP LOW it will be turned off by using the key LAMP OFF After turning on the discharge lamp will first be heated while the LED is flashing then the discharge lamp starts lighting and the LED is permanently lit The modes HIGH and LOW differ from each other by the current of the discharge lamp the quantity of light corresponds to the current and it is connected with a corre sponding level of noise noise as well The lifetime of the lamp depends on the value of the current The mode HIGH current is 150mA is convenient where it is necessary to have a minimum noise especially during the measurement at the wavelengths on the margin of the range where the level of the light is lower A disadvantage is a lower lifetime of the lamp in this case The mode LOW current is 100mA is advantageous in the installations where it is necessary to save the discharge lamp and the signal is so high that the increase in noise will not influence the accuracy of the measurement The manufacturer states the lamp lifetime as 1000 hours at a current of 300mA Even in the mode HIGH the lamp lifetime is several times higher INGOS s r o Page 12 LCD5000 Section 4 SERVICE The halogen lamp serves for the measurement in the area of VIS from 360nm to 700nm The lamp is
7. of 25 samples per second Keyboard and display The keyboard has 36 keys some of them are completed with an indication LED Its display is represented by an intelligent LCD featuring a background illumination facility it has two lines by 16 characters INGOS s r o Page 21 6 ACCESSORIES AND REPLACEMENT PARTS 6 1 Basic accessories 1 item Mains cord 1 item Communication cable RS485 2 items Fuse T 1 6 A 2 items Through bolt UNF10 PEEK 6 2 Other accessories and replacement parts Furthermore it is possible to order these accessories and replacement parts Halogen lamp Discharge lamp deuterium Through bolt UNF10 PEEK Pipe 1 16 0 25mm TEFLON Preparative cell Analytical micro cell INGOS s r o Page 22 7 WARRANTY The manufacturer provides a one year warranty period for the detector LCD5000 which starts running from the day when the product is handed over to the customer The apparatus can only be used in the way provided for in these instructions The ma nufacturer is not liable for any damage arising as a result of a failure to comply with the conditions provided for in the instructions This warranty does not cover the deuterium discharge lamp and the halogen lamp The apparatus is designed for the environment featuring a temperature of 15 to 35 C its humidity being up to 80 without any vapours of acids and caustic substances The operators must be made familiar with applicable saf
8. switched on by using the key VIS LAMP pressing this key again you will switch it off The fact that the lamp has been switched on is indicated through a LED on the keyboard If both of the two light sources are switched on at the same time the linearity during the measurement above 370nm is distorted at absorbency values higher than 0 2AU Therefore it is necessary to switch the discharge lamp off during the measurement above 370nm Both sources at the same time are used mainly for scanning through the entire spectrum when no exact quantitative assessment is necessary 4 1 2 Basic display This display will be activated by using the key ABS ABS 4 123456 1 z24B8nm t 1 685 The following data will appear on the display ABS Absorbency By using the key ZERO it is possible to reset zero the absor bency value 1 Set wavelength The wavelength can be pre set within a range from 0 to 700 nm For other details see If the setting phase is underway the display reads as follows Wrk t Time constant value The time constant value can be pre set within an interval from 0 04 to 2 50 s 4 1 3 Analogue output setting The display for the pre setting of the output to be used for the analogue recorder or integrator will be activated if you press the key ATTEN ABS 4 123456 HU 4 5 AUFS 2 88 The display contains the following data ABS Absorbency By pres
9. system is illustrated in fig 3 The REC connector is an analogue output for the connection of the recorder or the integrator with an analogue input The voltage level of the signal is 1V 1 Computer 7 Detector 2 Computer power supply 8 Pump 3 Keyboard connection 9 Interconnection of the communication 4 Monitor connection system RS485 5 Mouse connection 10 Pump and detector power supply 6 Converter RS232 RS485 Fig 3 Example of the chromatography system interconnection All connectors are mutually non interchangeable and thereby there is no danger of damage to the detector or periphery equipment connected if you are using the intercon nection cables delivered by the manufacturer 3 3 3 Keyboard and display The actual control shall be carried out by using the keyboard situated on the front panel the information being displayed on the display which is a part of the keyboard The meaning of individual keys is as follows INGOS s r o Page 9 LCD5000 Section 3 PUTTING INTO OPERATION UV LAMP HIGH UV LAMP LOW switches the deuterium discharge lamp on see ELI LAMP OFF switches the deuterium discharge lamp off see mw VIS LAMP switches the halogen lamp on and off see mw 09 PREAMP TEST Switches over to the mode PREAMP TEST see 15 RUN activates the program control of the cycle if the program is situated in the
10. INGOS Ltd SPECTROPHOTOMETRIC DETECTOR LCD5000 User manual PRAGUE Apr 11 2006 Producer Development Supplier and service Laboratory Instruments division of INGOS Ltd PiKRON Ltd INGOS Ltd Tel 420 296 781 683 K Nouzovu 2090 420 296 781 692 14316 PRAGUE 4 Fax 420 244 403 051 CZECH REP e mail instruments ingos cz c INGOS 2006 INTRODUCTION CAPABILITIES AND SPECIFICATIONS PUTTING INTO OPERATION SERVICE DESCRIPTION OF FUNCTION ACCESSORIES AND REPLACEMENT PARTS WARRANTY TABLE OF CONTENTS ORO GE Y Y Ll 1 INTRODUCTION Detector fitted with an analytical cell is designed for analytical and semi preparative liquid chromatography If the cell is exchanged the detector can be used for preparative chromatography and micro column chromatography Furthermore the detector is usable for the measuring of absorbency in the through flow cell everywhere where it is possible to draw a sample into the cell and measure the absorbency value both at a single wavelength and for a partial spectrum A two line display together with a 36 key membrane keyboard provide user s comfort for both direct control purposes and program control In addition to the control of the detector itself the program can also control additional equipment designed e g for fraction collector etc The detector is constructed as
11. able you to make a choice by moving cursor by your pressing ENTER the selected items will be confirmed 09 LIST makes it possible to carry out the inspection of the program list and the se lection of one of them for activation or editing If only one program has been programmed it is not possible to use this function INGOS s r o Page 10 LCD5000 Section 3 PUTTING INTO OPERATION CYCLE AUX OUTPUT ATTEN ABS these keys are used for the switching over of individual displays CTRL INSERT LINE DELETE LINE ration of the program 3 4 Switching the apparatus on Procedure during your switching the apparatus on is as follows these keys are used during the prepa 1 Perform electrical connection of the apparatus see B 3 2 9 N Connect the cell input and output see 8 3 1 Turn the apparatus on by using the switch situated on the rear panel see fig 2 and wait until the internal checks are completed 9o c Ou The apparatus is ready to measure After stabilising initial conditions reset absorbency by using the key ZERO When you see the basic display set the wavelength and time constant value see 2 Turn on the lamp of a corresponding wavelength see Turn on the computer and activate the integration s
12. an independently functioning apparatus and it can be connected to a centrally controlled purposeful assembly consisting of more apparatuses Serial contacts make it possible to connect more apparatuses into a complete assembly for HPLC or for other purposes They also make it possible to control and assess the entire assembly by using a master computer Besides serial contacts the apparatus is fitted with a connector with TTL signals for the control of peripheries which do not have any processor built in This manual describes installation operation troubleshooting maintenance and ser vice instructions for the detector LCD5000 INGOS s r o Page 4 2 CAPABILITIES AND SPECIFICATIONS 2 1 Capabilities Optical assembly with a high optical gain fitted with modern electronic equipment and software provides large application possibilities without the apparatus having to be treated Detector with a variable wavelength within a range of 190 370 nm fitted with a standard deuterium discharge lamp Detector with a variable wavelength within a range of 360 700 nm fitted with a discharge lamp Detector with a variable wavelength within a range of 190 700 nm fitted with a built in deuterium lamp and halogen lamp Analytical detector for high performance liquid chromatography HPLC with a stan dard stainless steel through flow cell featuring an optical length of 5 mm and a volume of 5 ul or micro cell optical length of 4 mm
13. and a volume of 2 ul Optional Detector pro semi preparative and preparative chromatography with a through flow cell silica Teflon featuring an optical length of 0 2 mm and a volume of 10 ul Optional 2 1 1 Data transmission Detector transmits data along a serial line and at the same time along an analogue line for recorder Detector is fitted with the communication system RS485 and alternatively also with RS232 For the purpose of controlling its optional equipment without their own intelligent systems the detector is fitted with the outputs including retrospective controls TTL level signals INGOS s r o Page 5 LCD5000 Section 2 CAPABILITIES AND SPECIFICATIONS 2 1 2 Specifications Wavelength range 190 to 370 nm with a deuterium discharge lamp 360 to 700 nm with a halogen lamp 190 to 700 nm with a discharge lamp and halogen lamp 60 wavelengths min Wavelength accuracy Lam Wavelength reproducibility 0 1 nm 20d k lt 4 nm Measurement range 1 to 2 AU NOISE A cad 1 1075 AU at time const 1 s i RENE 5 10 4 AU h Time constant value re Ete 0 04 to 2 5 s Analogue output senora with resolution 15 bit software makes it possible to assign ranges 0 01 0 02 0 05 0 1 0 2 0 5 1 2 AUFS Output for computer cope sepa rere RS485 alte
14. ce AA AAA 19 INGOS 5 Page 25
15. d lamp in general Follow the instructions in 4 3 3 Little light If you think that the intensity of the light is low carry out an inspection of the lamps according to 4 2 1 If it is necessary to replace a lamp follow the instructions provided for in or E38 4 3 4 Discharge lamp replacement If you are replacing the discharge lamp follow the below described procedure In fig 6 you can see the location and connection of the discharge lamp Caution Hold the discharge lamp always at its base part never touch the glass of the discharge lamp If you contaminate the discharge despite these precautions clean it with alcohol Replacement procedure Turn the apparatus off and disconnect it from the mains Unscrew the two screws holding the rear cover and remove the cover Disconnect the discharge lamp from terminals Unscrew the two nuts and remove the discharge lamp with the holder Release the socket on the holder and remove the discharge lamp Qu Ot dog Be deos Insert a new discharge lamp into the holder turn it in such a way that the outlet port of the discharge lamp will be in the same direction as the outlet port of the holder 7 Fix the discharge lamp in the holder by tightening the socket INGOS s r o Page 16 LCD5000 Section 4 SERVICE Tee EN 1 Discharge lamp base 4 Nuts serving for the attachment of the Socket used for the attachment of the discharge lamp holder
16. equire any maintenance other than the cleaning of the surface It shall be carried out with the help of a cloth moistened in soap water During the connection of the cell see B 3 1 it is necessary to carry out a careful inspection for leakage of the pipe connection to the cell at the first activation with a through flowing liquid This inspection is still to be carried out after a several hour long operation Any liquid which could possibly leak shall be collected on the bottom of the cell box Any possible leakage shall be removed by means of the tightening of the concerned connection The liquid which has escaped shall be fully removed by wiping 4 2 1 Inspection of lamps By using the function PREAMP TEST 1 5 you can control the intensity of the light in the reference branch REF Thereby you can assess the ageing of the lamps The deuterium lamp shall be controlled at a wavelength of 250 nm If the intensity of the light drops to one half or if the lamp starts burning unevenly the value is oscillating it is necessary to replace the lamp The manufacturer of the deuterium lamp guarantees a reduction of the luminous flux to one half after 1000 hours at a current of 300 mA The halogen lamp shall be inspected at a wavelength of 360nm If the lamp features unstable behaviour at this wavelength it needs replacing 4 3 Troubleshooting 4 3 1 Problems with the cell With the help of the function PREAMP TEST 1 5 you can identify the abso
17. etector on the table and make yourself familiar with the distribution and functions of the control and connection items The equipment does not impose any requirements on additional ventilation 3 2 What you need For the putting into operation of the detector you will need the following items 1 A pipe for the connection of the cell to the chromatography zone and a pipe designed for the offtake of the acid flowing out from the cell It is usually made from the material TEFLON external diameter 1 16 internal diameter 0 25 mm and passing screws for the pipe connections 2 Computer fitted with the assessment program and a converter for the detector con nection 3 Pump dispensing valve chromatography column Note If Teflon pipes are used the pipes must be fitted in a length of 50 mm in front of the cell with a light impermeable cover so that the external light shall not influence the measurement Black tubing can be optimally used as a cover 3 3 Control and connection items 3 3 1 Cell The cell is accessible through the door situated on the left side of the apparatus and it can be taken away after the loosening of the two nuts and removal of the photocell The orientation of the cell inlets according to 1 is the only one that operates when the inlet is situated on a bottom part The pipes shall be led through the cuttings situated next to the door 3 3 2 Rear panel The following elements are situated on the rear panel
18. ety regulations and with the methods of work with the liquids used All warranty and after warranty repairs shall be made by the manufacturer or by the organisation authorised by the latter Unless the delivery note provides for otherwise contact the manufacturer if you have any request concerning repairs 7 1 Waste disposal When the instruments operating life is over dispose it in respect to valid regulations also it can be returned to the vendor or producer for liquidation Warning Instrument contains parts PCB s which are rated as hazardous waste INGOS s r o Page 23 8 TABLE OF CONTENTS Li INTRODUCTION dicke bebe testet bait etie Edd ida 21 CAPABILITIES AND SPECIFICATIONS cece cece cece e FM apatites dias ETE Data anl TORTE 21I Specifications 2 UMA rita Pede pP dd pier 7 24 What you Ded 7 Control and connection items Eo td age a A A o Ebeb o o aan hides xd ite Fd rb ee bp obe deii b ductus 2 Ao MAP deeenhepebeceus qox pie pma taa 7 L 3 3 3 Keyboard and display D 3 4 Switching the apparatus On caus LT SERVICE ocre wv REm AAA eld eh sacer Gio dee OG doing ae 12 E ind aye CERERI ERE FR 12 ATA Basic display 13 ATA Analogue output setting
19. lute value of the light passing through the cell If the intensity of the light is low and there is enough light on the reference it signalises that the cell has been contaminated Cleaning can be carried out by using a syringe with the help of a liquid current While cleaning you can use connection pipes whose clearance is 0 8 mm Choose suitable liquid according to the type of contamination If the rinsing with a liquid current does not help it will be necessary to disassemble the cell and carry out mechanical cleaning of the cell body and lenses The lenses can be cleaned only with wadding it is also possible to use alcohol denatured with petrol or ether The cell assembly is illustrated in Figure fig 5 4 3 2 large noise Large noise could be caused by impurities in the cell or by a shortage of the light INGOS s r o Page 15 LCD5000 Section 4 SERVICE Monochromatic device Cell pad Cell body Lenses Internal area of the cell px Qv gm c9 B p Photo metering probe 1 2 3 45 65 5 0631 cross section By using the function PREAMP TEST 4 1 5 you can find out the reference and cell intensities of the light If the reference contains a sufficient light intensity there are impurities in the cell and you should follow the procedure according to 3 1 If there is little light in the reference it could be caused by your using a bad lamp for the wavelength in question see 1 1 or by a ba
20. oftware INGOS s r o Page 11 4 SERVICE All functions of the apparatus are controlled with the help of the keyboard and the display situated on the front panel The apparatus can be controlled in several modes They are based on direct control program run and program editing Individual modes are described below All control modes have certain common characteristics This concerns 1 Cursor movement It is possible to move the cursor by using the keys Cursor does not move by individual characters but by the items 2 Entering numerical data It is impossible to change the item under which the cursor is situated Numerical data is entered by using numerical keys If it is necessary to enter a negative number use the key CTRL After entering it is necessary to confirm the number by using the key ENTER 3 Choice from the menu If you are choosing from the menu move cursor to the item required and carry out the choice by using the key ENTER If the menu is longer you will see only two lines on the display and during the cursor movement the menu scrolls 4 1 Direct control 4 1 1 light sources The detector made in the UV VIS design has two light sources They are a deuterium lamp and a halogen lamp The detector in the UV design is fitted with a deuterium discharge lamp only The deuterium discharge lamp is used
21. onsist of top quality parts The design is optimised in such a way that high stability and reliability of the entire apparatus can be ensured The electronic system can be divided into the following parts Impulse mains source The input voltage of the source can be 110 to 230V 50 to 60Hz The output voltage is 24V All other units are constructed to a voltage of 24V Voltage source for the discharge lamp and lamp The source for the discharge lamp is an impulse converter featuring an input voltage level of 24V and output voltage level of 5 3V 2A The discharge lamp source consists of two parts actual source and auxiliary source for heating The actual source is of a current type 100 or 150mA maximum voltage level being 100V The heating source is of a voltage type 6 or 10V 2A During the ignition phase it is necessary to use a higher voltage during operation the discharge lamp is heated with a lower voltage The sources are controlled by means of a control unit Control unit This unit ensures the control of the entire apparatus its basic part is represented by the processor Siemens 80C517 32kB of EPROM memory and 32kB of RAM memory is connected to the processor The RAM memory serves as a storage area for user s programs that is why it features a battery backup INGOS s r o Page 20 LCD5000 Section 5 DESCRIPTION OF FUNCTION A D converter is of a special construction 24 bits The converter operates at a speed
22. rnatively RS232 Automatic resetting key or program command Control signals WOME E O 2 TTL level OUTPUTS EMT 4 TTL level Standard analytical cell optical length 5mm plo TET 5 pl Dimensions w x h x d 240 x 130 x 400 mm Weight qe 7 5 kg Power supply us vides ep eR 110 to 230 V 10 50 to 60 Hz Overvoltage category in the installation II Power input uias cer at cca ded 100 VA Noise and drift measured 1 h after turning on dry cell temperature drift 0 5 C with a standard cell 5 ul 5 mm and a wavelength of 254 nm The apparatus is designed for the environment featuring a temperature of 15 to 35 C humidity up to 8096 without any vapours of acids and caustic substances Any use of the apparatus other than the use provided for in this manual could en danger the operation safety INGOS s r o Page 6 3 PUTTING INTO OPERATION 3 1 Unpacking Unpack the detector carefully from the transport package Check the external parts of the apparatus for damage having possibly occurred during transportation If you find any damage contact the carrier The manufacturer s plate is located on the internal side of the cell door Check all items according to the Packing Note 6 1 If the real number does not correspond to the Packing Note check thoroughly all packing material If an item is missing contact the manufacturer or your supplier Put the d
23. s Detection photodiodes for the measurement and reference branches The apparatus uses PIN photodiodes featuring a high sensitivity also for short wavelengths 5 2 Mechanics From the mechanical point of view the detector is constructed in such a way that it can provide wide possibilities despite its small dimensions The construction also followed the possibilities of servicing The mechanical parts of the detector consist of the following items Case The case is made of hard aluminium and constructed in such a way that it can be as light as possible while keeping its maximum stiffness The front panel is formed by a standard plastic pressed piece similarly as in the case of other apparatuses of this series pump dispenser The monochromatic unit ensures through its robust construction high stability of the measuring system Wavelength adjustment mechanism This mechanism is driven by a stepping motor which rotates the micrometric screw The nut of the micrometric screw moves the lever connected with a grille This mechanism provides for the function arcsin This function is required for the transmission between the motor steps and the wavelength to be linear The connection between the stepping motor and the micrometric screw ensures an exact adjustment of the wavelength 1nm and the reproducibility of adjustment 0 1nm 5 3 Electronics Electronic parts of the detector are designed with regard to the use of modern pro cedures they c
24. sing the key ZERO it is possible to reset zero the absorbency value AUFS The first number specifies how many absorbency units belong to the entire range of the recorder The second number is the reciprocal value of the first number It states the factor with which the output signal is multiplied By using the keys 4 and W it is possible to set the range in firmly defined steps While setting the range on Usr it is possible to pre set also the multiplication factor the second number on an arbitrary basis INGOS s r o Page 13 LCD5000 Section 4 SERVICE 4 1 4 control inputs and outputs By using the key AUX OUTPUT you will activate the display used for controlling input commands and reading their feedback responses ALI m OUTPUT 1234 56 On the display you will read the following data S Input commands They are controlled by means of a numerical keyboard where the pressing of the keys 1 to 4 will initiate the command and a new pressing of the same will cancel it R Only the feedback responses are displayed 4 1 5 Preamp test This test serves for the testing of the discharge lamp verification of electronic equip ment and for the verification of a luminous flux by reference and the cell It will be called by the key PREAMP TEST Preame test Exit ABS 8 125456
25. velengths above 360nm In order to achieve a maximum evenness of the luminous flux over the entire spectrum the combination of the light coming from individual lamps uses a special semi permeable mirror tailored to our apparatus In order to improve the evenness still more a filter has been inserted in front of the halogen lamp This filter serves also for the de filtration of short wavelengths originating from the lamp light which could result in measurement inaccuracies in a visible area see Rotary attached holographic grille T his grille serves for the wavelength selection A top quality grille produced by the firm Karl ZEISS has been selected This grille ensures a small half width and an exact adjustment of the wavelength 1 oO T 8 9 40 43 ANM Q S 1 Deuterium discharge lamp 8 Output reference diaphragm 2 Semi permeable mirror of the combi 9 Semi permeable mirror of the separa nation device tor 3 Filter 10 Output diaphragm of the measurement 4 Halogen lamp branch 5 Input lens 11 Cell 6 Input slot 12 Measurement photodiode 7 Reference photodiode 13 Grille Fig 8 Optical chart of the detector INGOS s r o Page 19 LCD5000 Section 5 DESCRIPTION OF FUNCTION Separator for the division of the light into the measurement and reference branches This separator is formed by a special semi permeable mirror which ensures good proper ties for all wavelength
26. ys at its base part never touch the glass of the lamp If you contaminate it despite these precautions clean it with alcohol p em ww mrs sr a 8 eee Y 1 Lamp 3 Terminal board for the connection of Discharge lamp the discharge lamp 4 Terminal board for the connection of the lamp Fig 7 Location of the lamp and discharge lamp Turn the apparatus off and disconnect it from the network Unscrew the two screws holding the rear cover and remove the cover Unscrew the four screws holding the upper cover and remove the cover Disconnect the lamp from the terminals Unscrew the two screws holding the lamp and remove the lamp Insert the new lamp and screw it It is not necessary to adjust the lamp Mount the upper and rear covers Noo og NE INGOS s r o Page 18 5 DESCRIPTION OF FUNCTION 5 1 Optics The optical set is constructed in such a way that it can ensure the adjustment of the wavelength as exactly as possible and at the smallest half width possible The use of lenses and mirrors made of high quality silica glass ensures that the loss of light will be very small even on the short wavelengths about 200nm Optical set fig 8 consists of the following parts Light source In order that the apparatus will be able to work in the entire spectrum of wavelengths the source is formed by two lamps They are the deuterium discharge lamp for wavelengths below 370nm and the halogen lamp used for wa
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