VEP-2 and VEP-3 Tank Electroblotting System User Manual
Contents
1. sujuouu xis Auiu 38114 ayy 6uung Jeuwo juenbesqns Aue uonoajold jueJem 99 si jueuudinbe noA y 10948 jueJem y os Buiddius smojje siu uno peddius si jueuudinbe eui SY M OM AUeJeM 941 VSN SLONGOYd IMO OISILNAIDS 43HSIA OINH3HL 8 1 Tank Electroblotting System Thermo Scientific Section 8 Warranty Information L006 OSI cL 6 0 ed uoneuuojul AAUELEM JO Jojnqujsip Jno YSN eui episino suoneordde jeloeds pue eoiAJes uoneJedo AjueJem Juswd nba uo suonsenb noA Jamsue Jo epeueyd Jo YSN 158y 8 py 008 1 Je 1ueunqedeq sanas e2iuuo9 Jno jeo aseajd peJuinbaJ s eoueuojureuJ eAnueAeJd pue uonejejsur 3ueuudinbo AjjnJoued uononujsur jueuudinbe 8J0Jeq uoneuuojul uoneJedaud eis SAISUBYSJGLUOD YIM djay Apes si sejes 9 INOA SjonpoJd sso sold 150 0 sebewep jenuenbesuoo Jo apui Aue eq jou eys oway AlddV TIVHS YVINOILYVd2. Filter paper should be saturated with transfer buffer before adding them to the sandwich Reducing methanol can help elute proteins from gel 5 2 Tank Electroblotting System Thermo Scientific Section6 Care and Cleaning A few tips about caring for your system follows Caution Organic solvents cause acrylic to craze or crack Clean all acrylic systems with warm water and a mild detergent Do not use ethanol or other organic solvents to clean these products Do not autoclave bake or microwave your unit Temperatures over 50 C can damage the acrylic Note If an RNase free electrophoresis system is desired there are various methods to rid the system of RNA contamination For fast and easy decontamination use RNase AWAY Spray wipe or soak labware with RNase Away then wipe or rinse the surface clean it instantly eliminates RNase RNase Away eliminates the old methods that include treatment with 0 1 Diethyl Pyrocarbonate DEPC treated water and soaking in dilute bleach DEPC is suspected to be a carcinogen and should be handled with care This electrophoresis system should never be autoclaved baked or placed in a microwave A To order RNase AWAY contact Technical Services Part Description 7000 Luce aae e S 250ml botte 7002 475ml spray bottle TODS AS ae Baal le A RN 1 liter bottle weed e Ea T XS 4 liter bottle
3. Rnase AWAY is a registered trademark of Molecular BioProducts Thermo Scientific Tank Electroblotting System 6 1 Section 6 Care and Cleaning Care of Acrylic The following chemical compatibility chart is supplied for the convenience of our customers Although acrylic is compatible with most solvents and solutions found in the biochemical laboratory some solvents can cause substantial damage Keep this chart handy to avoid harm to your apparatus by the use of an inappropriate solvent Codes S Safe no effect except possibly some staining A Attacked slight attack by or absorption of the liquid slight crazing or swelling but acrylic has retained most of its strength U Unsatisfactory softened swollen slowly dissolved D Dissolved in seven days or less 6 2 Tank Electroblotting System Thermo Scientific Section 6 Care and Cleaning Table 6 1 Chemical Compatibility for Acrylic Based Products ne s EEE fa mm Recens E eem emm emo CC 0 _ p O CA 5 _ p je CI 5 _ DN ETC uo CEI je EEC E CNN CN femme CE Er je CN _ ESCONDE O EXE UN COEN IE CU _ O je CO pm _ This list does not include all possible chemical incompatibilities and safe compounds Ac
4. siu uno peddius si juawdinba ajep ay suyuouJ OM SHES AjueJueM eu TWNOILVNYSLNI SLINAOUAd IMO 2131133195 S3HSI3 1 Thermo Scientific Tank Electroblotting System 8 2 Thermo Fisher Scientific 401 Millcreek Road Marietta Ohio 45750 United States www thermofisher com ThermoFisher SCIENTIFIC
5. be sure that the power supply you will be using will work with this device Some power supplies that will work with this device are Owl s OSP 135 Owl s OSP 300 EC Apparatus EC135 and EC570 and Bio Rad s PowerPac 200 If your power supply is not among those listed contact your power supply manufacturer to determine if it will work for tank blotting applications Contact Technical Services for power supply recommendations if you do not have an appropriate power supply Tank Electroblotting System 2 1 Section 2 Setting Up 2 2 Materials Needed continued Setting Up the Blot Tank Electroblotting System Blotting Buffer See the Buffers section page 14 of this manual for buffer recipes The most commonly used buffer for protein blotting from poly acrylamide gels is Towbin buffer Small amounts of buffer may be needed for equilibrating the gel and membrane prior to blotting in addition to the buffer in the transfer tank Buffer should be cooled to 4 C Filter Paper Sometimes called blotting paper it is used in the blotting sandwich It can come as pre cut thicker blotting sheets such as part number FP 4 or large sheets or rolls of Whatman 3MM paper can be cut and used Blotting Membrane Nitrocellulose and PVDF Polyvinylidene difloride Hua can be used for Proteins while charged Nylon membranes can be used for nucleic acids The choice depends upon the user s preference and sometimes the detection meth
6. Order 1 1 Setting Up isis 2 1 Materials Needed 12 5 9 Sarid os Prussia Sed he doh eG pas ak Ras 2 1 Seting p the Bl t tea end elite raed 2 2 Using the System 3 1 Running the Bot zu edv tenon sy Eau 3 1 Transfer Settings S s Verde O 3 1 Factors that Affect Transfer Efficiency 3 1 Technical Tips 2 2 ren 4 1 Different Kinds of Blotting 4 1 Recipes for Buffers til e balas 4 2 Troubleshooting He an 5 1 Care and 6 1 Optional 7 1 Tank Electroblotting System V Section 1 General Information The VEP 2 Tank style Electroblotting System is designed to provide uniform reproducible protein transfers over a wide molecular weight range Tank style blotting is highly efficient Efficient transfers of up to four polyacrylamide mini protein gels are possible simultaneously in this robust system The integrated cooling base allows temperature controlled runs Stir bars may be added to the bottom of the buffer chamber to allow for increased buffer circulation and heat exchange This is the perfect addition to any mini protein system The VEP 3 Tank style Electroblotting System is designed to provide uniform reproducible protein tran
7. V SSANLIS HO ALETISVENVHOSSIN S3LENVSHSHVM ON Gal Idi WHO N3 LLIHM YSHLSHM S3ILLNVHHVM H3HLO TIV NI ANY 3AISNTOXA SI ALNVHSHVM SIHL uoneunsep gO peddius sued pue pied aBejsod oj peuunjeJ eq sued Buruuojuoo uou uondo 5 yy jueuudinbe Jo Aue 9AID juawuedag eoiuuo9 94 poled jueJem ay Jusuodwos y Jo jueuudinboe jueJem ay jou jUeJem siu Jepun jueuudinbe Jo sued Jo edau JO JU amp JIeM SIU pepnjoxe sjexseb pue sia 552 6 sway ejqepuedx3 Aue jo eoueuuo0J 19d Joud pue uoneuiuuJejep JUeJJem JO eq juaewuedag seorues eoiuyos 941 Aueulem Aq P819A09 jou SI pue uoneJqijeo 6undeoxe esuedxe s ouueu peoe doiJ d ysuewy Jom Jo ui ue oJd sued sujuouuJ 18414 ayy Buunq Jeuwo juenbesqns uoi 98 01d s juaudinba noA ay A ojeuurxoJdde Pays ojul jueJem sy os Buiddius
8. clean before putting the rest of the filter paper on the sandwich Transfer will not occur where the gel is not in contact with the membrane Running conditions sample preparation percentage acrylamide and many other variables can affect the migration and resolution of proteins Please review your electrophoresis condi tions Always pre wet the membrane according to the manufacturer s instructions White spots indicate dry areas of the membrane Too much current Running at constant voltage can cause power fluctuations that will cause overheating A buffer that has not been made correctly or that has too high in ionic strength can also burn a gel by overheating A cracked and dry gel often is an indicator of overheating Thermo Scientific Tank Electroblotting System 5 1 Section 5 Troubleshooting Problem continued Solution continued Transfer efficiency is poor Power supply is appropriate for transfer EN Transfer performed for too short a time Transfer sandwich was assembled in the wrong order The pH of the transfer buffer is too close to the iso electric point of the protein Too much methanol in the transfer buffer High percentage gels restrict transfer Try a more acidic or basic transfer buffe but can reduce binding to nitrocellulose membranes Higher percentage acrylamide or crosslinker can restrict elution of proteins Use the lowest percentage acrylamide possible to separate your proteins
9. Ie 13cm W x 16 3cm H x 18 5cm D Buffer required 1300ml Gel size maximum 10cm x 10cm Current requirements 200 500mA Time required 45 minutes to 2 hours Model VEP 3 Dimensions Footprint 30cm W x 30cm H x 9 D Buffer required 4 0L Gel size maximum 20cm x 30cm Current requirements 1 5 A Time required 60 120 min Tank Electroblotting System 1 3 Section 1 General Information 1 4 Tank Electroblotting System POWER SUPPLY _ LEAD 2 da zx mn FETY nai fk CASSETTE CLIP 4 I BUFFER 7 CHAMBER Figure 1 2 VEP 3 Exploded Parts Diagram Table 1 2 Parts List pee Lid with attached Power Supply Leads Buffer Chamber with Stainless Steel Cathode Plate and Titanium Anode Plate Blotting Cassettes with Foam Pads Blotting Filter Paper 20cm W x 18 5cm L Cooling Lid Thermo Scientific Materials Needed Thermo Scientific Section 2 Setting Up Once the proteins or nucleic acids in a sample aliquot have been separated on a slab gel the resulting bands may be transferred to a solid support membrane The primary reason for this type of blot is one of localization and secondarily concentration of discrete protein bands Although many have used alternative cross linking agents such as DATD N N dihydroxyethylene bis Acrylamide to allow for th
10. Tank Electroblotting System Models VEP 2 and VEP 3 Operating and Maintenance Manual 7007351 Rev 0 Visit us online to register your warranty www thermoscientific com warranty o Owl 37 Preface MANUAL NUMBER 7007351 0 4 30 12 Transfer to Marietta was The Bandit 3 2003 CCS REV ECR ECN DATE DESCRIPTION By Thermo Scientific Tank Electroblotting System i Preface CAUTION Contains Parts and Assemblies Susceptible to Damage by Electrostatic Discharge ESD Important Read this instruction manual Failure to read understand and follow the instructions in this manual may result in damage to the unit injury to operating personnel and poor equipment performance Caution All internal adjustments and maintenance must be performed by qualified service personnel Warning To avoid the risk of personal shock always disconnect the gel box from the power supply Further the power supply must be equipped with a shut down on disconnect circuit Running conditions for this unit should not exceed the name plate readings found on the lower buffer chamber Do not move the unit unless the power source to the unit has been disconnected Statement of Proper Use Use this product only for its intended purpose as described in this manual Do not use this product if the power leads are damaged or if any of its surfaces are cracked This system is designed to meet IEC 1010 1 safety standards IEC 1010 1 is an internati
11. Y Cathode gt 5 5 o c 9 5 g Figure 2 2 VEP 2 Cassette o Filter Paper Figure 2 1 Blot Assembly Thermo Scientific Tank Electroblotting System 2 3 Section 2 Setting Up 2 4 Setting Up the Blot continued Tank Electroblotting System 14 15 16 17 Add another filter pad and complete the sandwich with a Scotch Bright pad Close and lock the cassette The VEP 2 uses a black C clamp that is pressed over the two open ends of the cassette opposite the hinge The VEP 3 has an integral lock which when the red half of the cassette is lowered into position the lock is slid to the right engaging both halves of the cassette Add the cassettes to their respective slots in the buffer tank Place them into the tanks already filled with cold buffer 4 C with the clamps facing up The VEP 3 will fit into the buffer tank one way to ensure that the polarity of the cassette is correct Match the red cassette side to the white or red bars on the inside of the chamber If the cassette is reversed it will stick out of the chamber and the lid will not fit Thermo Scientific Section3 Using the System Running the Blot 1 Adda stir bar to the buffer chamber 2 Place the lid with power leads on the unit 3 Attach the power leads red to red black to black to an appropriate power supply 4 Run the blot Transfer Settings Blotting takes place at a given
12. d buffer for protein transfers to nitrocellulose membrane 0 025M Tris Base 0 192M Glycine 5 20 MeOH pH 8 3 1X Tris Borate EDTA Buffer TAE 1X TBE is used for agarose and polyacrylamide gel electrophoresis and semidry electroblotting of of nucleic acids Final 1X composition 0 04M Tris Acetate 0 001M disodium EDTA pH 8 0 Thermo Scientific Section5 Troubleshooting Smeared or swirled transfer and missing bands Nitrocellulose membranes Over transfer through the membrane Low MW proteins are not binding well or are being washed away SDS is preventing binding PVDF Membrane was dried out before it was added to the transfer sandwich Alcohol was not used to prewet the membrane Use 0 2 micron pore size nitrocellulose instead of 0 45micron or use PVDF with a higher binding capacity Use glutaraldehyde to crosslink the proteins to the membrane and use Tween 20 in the wash steps Eliminate SDS in the transfer buffer Membrane should be completely gray and slightly translucent when added to the sandwich If it has dried out re wet in methanol and equilibrate in transfer buffer PVDF is hydrophobic and requires a short soak in methanol prior to transfer Electrophoretic conditions were incorrect not ideal gel after transfer Transferring at too high a current Membrane was not thoroughly saturated Roll a test tube or pipet over the membrane make sure it is
13. e accessibility of gel bound proteins this still represents an impediment to radio enumeration due to the quenching by the gel matrix itself The most common solid support membrane is nitrocellulose A second type of membrane is PVDF Polyvinylidene difloride which is generally used when a transferred protein is to be sequenced additionally it has a 2x binding capacity Also used for nucleic acid capture are nylon membranes In either case the proteins are transferred from the gel to the matrix in an electric field perpendicular to the gel initial running direction Tris based buffers are employed in the transfer Methanol and SDS are modifiers often use in protein transfer buffer These components however are antithetical in their effects both in terms of movement and adsorption Methanol restricts protein movement from the gel but is often required to support the ionic nature of protein to nitrocellulose binding SDS aids in protein elution but can also inhibit binding of small molecular weight proteins Mozdzanowski J High yield electroblotting Electrophoresis 1992 Vol 13 p 59 64 In order to use this blotting device you will need Power supply Blotting requires a power supply that can operate at a fairly high current setting and low voltage If an inappropriate power supply is used the power supply may blow a fuse shut itself off display a no load or short load message or even have a short circuit It is very important to
14. entific Section 2 Setting Up Setting Up the Blot 7 Wet the membrane according to its manufactures recommendations H followed by a quick equilibration in transfer buffer It is often helpful continued to have all the filter paper and membranes sitting in transfer buffer as you start to build the blotting sandwich 8 The red and black sides of the transfer cassette are hinged and open up to a convenient angel for building a gel sandwich This is best done in a dish or shallow plastic box Place the cassette with the black side down 9 Lay Scotch Bright pad on the black half of the cassette followed by a filter pad Both should be soaking wet with buffer In fact the tray may end up filled with buffer as you build the blotting sandwich 10 Add a few mL of buffer to the filter pad and gently layer the gel Beginning at one end of the gel align the filter pad with the gel edge and slowly lower the other end driving out any bubbles 1 Wearing gloves gently smooth out any bubbles by forcing them to the closest edge of the gel Test tubes and pipettes many also be used for this purpose 12 Alternatively you can place the filter pad in a box with buffer toss in the gel and drain the box until the gel falls to the pad Then pick up the two and layer them on the Scotch Bright pad 13 Add a few mL of buffer to the gel and gently layer the membrane as you did the gel Transfer mE 1 SAATI AA
15. migration rate for a specified time The units are mA times hrs If you need to slow the transfer down to say coincide with the setting up of a probe simply decrease the current mA to match the added time you require mA hr Std setting mA hr New setting Alternatively one can increase the current to decrease the time This assume that you have determined an initial mA h value that works well for the molecules you are interested in Factors that Affect While general conditions can be described which will result in successful Transfer Efficiency transfer of most molecules it should be noted that optimal transfer conditions will vary based on the characteristics of the molecule you are working with Some factors that affect transfer rate and efficiency include molecule size charge gel thickness and percentage and hydrophobicity The reference list at the end of this manual provides useful information that can help you choose optimal conditions for efficient transfer of a specific molecule Thermo Scientific Tank Electroblotting System 3 1 Different Kinds of Blotting Thermo Scientific Section 4 Technical Tips How long will it take to blot the proteins from my gel Transfer times have to be determined experimentally This is because transfer time is dependent upon Percentage of gel Type and amount of cross linking in the gel Type of protein cytoplasmic membrane nuclear Size of protein There is n
16. o formula for determining transfer time There are too many variables involved to give specific transfer conditions that will work for every protocol Guidelines are VEP 2 400mA for 2 hours VEP 3 1200mA for 2 hours These guidelines are just a starting point and exact conditions have to be determined Western Blotting is a blotting method for proteins that use specific antibodies attached to a peticular protein to help identify it It is often performed after SDS PAGE or some other form of polyacrylamide gel electrophoresis Southern Blotting is a method sometiems called hybridization because a radioactive probe is hybridized or attached to specific pieces of DNA Northern Blotting is a similar method but the molecules involved are RNA Both Southern and Northern blotting generally require the DNA or RNA to first be separated out of an agarose gel Tank Electroblotting System 4 1 Section 4 Technical Tips Recipes for Buffers 4 2 Tank Electroblotting System 1X Tris Borate EDTA Buffer TBE 1X or 0 5X TBE is used for agarose gel electrophoresis and semidry electroblotting of nucleic acids Final 1X composition 89mM Tris Base 89mM Boric Acid 2mM disodium EDTA pH 8 3 1X Towbin Buffer 1X Tris glycine buffer Towbin buffer minus the methanol is used for agarose and polyacrylamide gel electrophoresis of nucleic acids with PVDF membrane Towbin buffer containing 5 20 methanol is a commonly use
17. od to be used Also available is Immobilon P PVDF transfer membrane in a mini gel size 10 x 10cm catalog TM151 5 After electrophoresis remove the gel assembly from the apparatus and remove the spacers Open the gel cassette by gently rocking a spatula between the plates forcing separation of the plate from the gel The gel will normally remain affixed to the bottom plate Remove the top notched plate by slowly lifting it from the side with the inserted spatula and gradually increasing the angle until the plate is completely separated from the gel If the gel sticks to the top plate in an isolated spot a stream of water from a squirt bottle can be sprayed at the spot to aid separation Remove the gel from the remaining plate Tip the plate up side down and start one edge and allow it to roll off into transfer buffer Alternatively place the plate with the gel attached into transfer buffer Incubate the gel in transfer buffer for 15 min with gentle agitation If the gel is on the plate it will become loose during this step Wearing gloves cut the membrane to the size of the gel and blotting paper Mark the membrane to indicate the side to which the samples will be on This is important in the event that any successive probe is negative and to indicate sample orientation This can be done by either clipping a corner of the membrane or using a ball point pen Clip the same corner until you retire continued Thermo Sci
18. onally accepted electrical safety standard for laboratory instruments Material in this manual is for information purposes only The contents and the product it describes are subject to change without notice Thermo Fisher Scientific makes no representations or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental arising out of or related to the use of this manual 2012 Thermo Fisher Scientific All rights reserved Tank Electroblotting System Thermo Scientific Preface Important operating and or maintenance instructions Read the accompanying text carefully gt gt gt Potential electrical hazards Only qualified persons should perform procedures associated with this symbol re gt Equipment being maintained or serviced must be turned off and locked off to prevent possible injury Hot surface s present which may cause burns to unprotected skin or to materials which may be damaged by elevated temperatures Marking of electrical and electronic equipment which applies to electrical and electronic equipment falling under the Directive 2002 96 EC WEEE and the equipment that has been put on the market after 13 August 2005 ERA This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo Fisher Scientific has contracted with one or more
19. oting of your equipment We can fill your needs for spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over the telephone without a service call When more extensive service is necessary we will assist you with direct factory trained technicians or a qualified service organization for on the spot repair If your service need is covered by the warranty we will arrange for the unit to be repaired at our expense and to your satisfaction Regardless of your needs our professional telephone technicians are available to assist you Monday through Friday from 8 00 a m to 6 00 p m Eastern Time Please contact us by telephone or fax If you wish to write our mailing address is Thermo Fisher Scientific 401 Millcreek Road Box 649 Marietta OH 45750 International customers please contact your local Thermo Scientific distributor iv Tank Electroblotting System Thermo Scientific Thermo Scientific Section 1 Section 2 Section 3 Section 4 Section 5 Section 6 Section 7 Table of Contents General Information 1 1 Unpack and Check Your
20. recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo s compliance with this directive the recyclers in your country and information on Thermo products will be available at www thermofisher com Y Always use the proper protective equipment clothing gloves goggles etc Y Always dissipate extreme cold or heat and wear protective clothing Y Always follow good hygiene practices Y Each individual is responsible for his or her own safety Thermo Scientific Tank Electroblotting System iii Preface Do You Need Information or Assistance on Thermo Scientific Products If you do please contact us 8 00 a m to 6 00 p m Eastern Time at 1 740 373 4763 Direct 1 800 438 4851 Toll Free U S and Canada 1 877 213 8051 FAX http www thermoscientific com Internet Worldwide Web Home Page service led marietta thermofisher com Tech Support Email Address www unitylabservices com Certified Service Web Page Our Sales Support staff can provide information on pricing and give you quotations We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you Our products are listed on the Internet and we can be contacted through our Internet home page Our Service Support staff can supply technical information about proper setup operation or troublesho
21. rylic products should be cleaned with warm water a mild detergent such as Alconox and can also be exposed to a mild bleach solution 10 1 In addition RNAse removal products are also safe for acrylic Contact Technical Services with any questions Thermo Scientific Tank Electroblotting System 6 3 Section 7 Optional Equipment Contact Technical Services to order replacement parts Item Description Complete System Accessories Power Supply Leads Blotting Cassettes VP2 BC Foam Pads pkg of 10 Blotting Filter Paper 10cm x 10cm pkg of 100 LN EE m Blotting Filter Paper 20cm x 20cm pkg of 100 m ESTI ZN Blotting Filter Paper 10cm x 10cm pkg of 100 Thermo Scientific Tank Electroblotting System 7 1 Section 8 Warranty Information re EIE SEN L006 OSI 21 6 0 uoneuuojul JUeJJem JO Jojnqujsip 890 Jno JOeNUOD YSN eui episino elo eds pue sonas uoneJedo AjueJeM Juswd nba uo suonsenb Jamsue Apea 9J 9M 9 7 EZE OPZ L epeueo pue YSN 1S8y 8 y 008 1 jJueuuedag sesinas p nb s sones Juswd nbe eAnueAeud pue uonejejsur jueuudinbe UONONI SU seAuJe yuswd nba uoneuuojul uoneJedoud aus SAISUBYSJGWOD YIM djey si seje
22. s INOA SjonpoJd Jo sso JO SyJOJA 180 uonejyuui jenuenbesuoo Jo Aue JO aq jou eys AlddV TIVHS 5 YVINOILYVd SSANLIS HO ALITISVLNVHOUFN S3LENVSHSHVM ON Cal Id WHO N3LLIHM YSHLSHM S3ILNVHSHVM H3HLO 11 N4 GNV 3AISQTOX3 SI ALNVHSHVM SIHL uoneunsep go4J peddiys sued 1ueujeoe daJ pue pied eDejsod ouueu L peuunjaJ eq sued BuruuJojuco uou uodo s ouueu 1ueuudinbe Jo jueuoduuoo Aue JO Jsnw jueumiedegq seorAJeg e2iuu29 99 ay puo eq y JO JOYS uenem y JOU uenem siy Jepun Jo sued 1ueuoduJoo Jo Jeda JO jueujeoe des JU amp JIeM 514 pepnjoxe sjexseb pue sia 552 6 ejqepuedx3 Aue jo eoue oj Joud pue JO jsnu sanas 941 siy Aq jou SI pue peoejdoJ diysuewyJom Jo ue oJd syed
23. sfers for larger sized gels or multiple mini gels This system provides efficient transfers of polyacrylamide protein gels A removable cooling block allows for efficient heat transfer Stir bars may be added to the bottom of the buffer chamber to circulate buffer and provide consistent cooling The cassettes are coded to ensure proper anode cathode orientation The cassette is designed to allow for easy loading of the gel sandwich and locks securely for loading into the buffer chamber These features make this the preferred system for large gel blots Tank systems are also recommended for large molecular weight proteins gt 150KD Unpack and Check Before starting unpack the unit and inventory your order If any parts are Your Order Missing contact Technical Services immediately Reference the order or catalog number on your invoice and check the corresponding parts list Thermo Scientific Tank Electroblotting System 1 1 Section 1 General Information 1 2 Tank Electroblotting System SAFETY INTERLOCKING LID POWER SUPPLY LEAD 2 CASSETTE CLIP 4 FOAM PADS 4 CASSETTE 4 N BUFFER CHAMBER Figure 2 1 VEP 2 Exploded Parts Diagram Table 1 1 Parts List Thermo Scientific Thermo Scientific Section 1 General Information Specifications and Recommended Running Conditions Model VEP 2 Dimensions Footprint 5 125in W x 6 44in H x 7 3in D io Fick EXAM Rd Sorgen D e EU CES
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