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CellaVision® DM1200 User`s Manual 3.0

1. Slide status Incomplete 4 analysis Could not find RBC monolayer Slide status Analysis failure View the Slide Information dialog for more details Oil on the slide No monolayer Clean the oil off the slide and process it again Make sure that the slides are prepared found according to the recommendations in Appendix G Oil on the slide Clean the oil off the slide and process it again Oil or dust on the objective Perform weekly maintenance on objectives see 10 1 1 Cleaning of Objectives and LED table Images are not in focus Dust on the objective Perform weekly maintenance on objectives see 10 1 1 Cleaning of Objectives and LED table Incorrect objective settings Contact your local vendor s technical support Seemingly normal slides get slide status Empty or no slide PID The barcode reader is not connected Check the cabling of the barcode reader No barcode found Check positioning and size of the barcode see Appendix A System Specification The barcode could not be read due to bad print quality Clean the barcode printer head Replace the printer ribbon on the barcode printer User s Manual 113 CELLAVISION o CellaVision M DM1200 Database access is slow The database server needs to be restarted Restart the system computer or the computer on which the database is stored
2. The database is too large Keep the database size at the recommended level see 10 4 Database Performance The database files are fragmented Compress the database see 9 1 5 Compressing a Database Seemingly normal scan slides get slide status Analysis failure with the additional information No image data in scanned area Too little specimen Prepare a slide with more specimen on the slides Wrong area Select the correct area and process the selected slide again 114 User s Manual CELLAVISION CellaVision DM1200 12 2 4 LIS Errors User s Manual Problem Slide status A Default values Cause The orders are not in the LIS Action Make sure that orders for the slides that are to be processed are already available in the LIS The slides are The connection to the Make sure that the cable to the LIS is analyzed with default LIS is broken connected values even though LIS is used for order information The connection to the Make sure that the cable to the LIS is LIS status q LIS has been broken connected Results are automatically resent when connection is established Critical error in the database The Order ID contains more than 24 characters Do not use an Order ID with more than 24 characters Could not find the order in the LIS Leading spaces in the Order ID Remove leading spaces in the Or
3. are 01200 Peripheral Bloud and Body Huns Rel celi n Gallery 2 Rot cel in Golewy 3 Worklist Non WBCs panel Add WBC 3rd gallery comment Cont d User s Manual 31 TM CellaVision M DM1200 Cont d All cell classes handled by the system are displayed in the figure on the following page WBCs and non WBCs automatically preclassified by the system are marked with a small dot or an arrow In settings see 9 4 Adjusting Reclassification Settings you may choose to auto forward preclassified WBCs to another cell class as follows Band neutrophil to Segmented neutrophil Metamyelocyte to Segmented neutrophil Lymphocyte variant form to Lymphocyte Plasma cell to Lymphocyte Blast cell Metamyelocyte Myelocyte and Promyelocyte to Other 32 User s Manual CELLAVISION CellaVision DM1200 An arrow indicates that pre classified WBCs are auto forwarded to another cell class for settings see 9 4 Adjusting Reclassification Settings Place the cursor over the arrow to see the destination cell class Arrow indicating an auto forwarded cell class Destination cell class Preclassified by the system WBC panel Non WBC panel WBC Unidentified fF Band neutrophil v Segmented neutrophil phil Eosinophil Basophil Lymphocyte Monocyte Promyelocyte Myelocyte Metamyelocyte Immature eosinophil Immature basophil Promonocyte Prolymphocyte f Blast no
4. Note CellaVision DM must be idle during export Otherwise the export will not be complete Export Log Files E Export of all log files in the program Export to CD C Export to LAN Export Burn Status 6 6 Backup and Recovery of the Database It is important to backup the database quite often If a hard disk crash occurs the whole database will be lost It will only be possible to recover the data up to the time when the last backup was made For more information see CellaVision IT Configuration Guidelines or contact your distributors User s Manual 69 NN CellaVision M DM1200 70 User s Manual e CELLAVISION 7 Digital Slides With CellaVision DM1200 it is possible to create digital slides Important The digitalized image generated provides a general overview of the prepared sample within the intended use of the CellaVision DM1200 applications All other use is for research use only and not for use in diagnostic procedures 7 1 Scanning a Slide Make sure that a Scan Database is available on your CellaVision DM To create a Scan Database see 9 1 1 Creating a New Database Log on to a Scan Database see 2 1 Starting the System To customize your system such as scan area and magnification see 7 4 Customizing the System 7 1 1 Slide and Magazine Requirements Requirements for the slides used when scanning Glass e Size in mm 75 0 76 0 x 25 0 26 0 x 0 9 1 2 Ground
5. Statistics Specification 2008 08 09 10 30 01 Installation File creation 2008 08 09 10 30 01 ast modified 2008 08 21 16 48 02 erial number Prototyp3 Nbr of analyses Nbr of failed analyses 18 18 Nbr of signed WBC 40 40 Nbr of signed RBC 37 37 Nbr of signed PLT 27 27 Total analysis time 312 50 312 50 The Statistics page contains dates and times of program installation and creation and modification of the Usage log It also displays the number of successfully processed and failed slides the number of signed analyses and the total processing time The information can be selected for a specific month by entering Year and Month and clicking Update Year 2008 Month 8 Update Print 6 4 2 Specification The Specification page is only accessible to Administrators It contains a list of dates and times for events that have occurred in the system e g start and completion of slide processing and hardware malfunctions You may specify the month the same way as described above 68 User s Manual CELLAVISION 6 CellaVision DM1200 6 5 Export Log Files It is possible to export all logging information from the system If a system error occurs the information is needed for trouble shooting Select 7ools Export Log Files The log files can be burnt directly to a CD or exported to the LAN and then for instance attached to an e mail The export log files dialog is only accessible to Administrators
6. Print order _ wes 2 If not prefilled type Username and Password 3 Select whether the order should be sent to the LIS and or printed 4 Click OK 5 4 Sending an Order to the LIS If the LIS is activated in the Analysis tab in Settings it is possible to send signed order results to the LIS 1 Open a signed order 2 Select the Slide Merge tab in the Report View 3 Click Send to LIS User s Manual 55 TM CellaVision M DM1200 5 5 Canceling an Order To cancel an order sign the order with no included slides 1 Open the order in the Database View 2 Select the Slide Merge tab in the Report View 3 Make sure no slides are included 4 Click Sign Order The Sign Order dialog appears 5 Sign the order as described in 5 3 Signing an Order result Note When the order is sent to the LIS the order will be reported as cancelled 56 User s Manual e CELLAVISION 6 Database and Archiving Data Click Database View in th toolbar Processed and pending orders are stored in the database Switch between the two using the tabs Processed Orders and Pending Orders 6 1 Database Processed Orders You can search for and open processed orders and slides stored in the system Orders are displayed according to the Search Criteria For more information on Search Criteria see 6 1 5 Searching for an Order Slide Cont d User s Manual 57 Cei CellaVision M DM1200 Cont d In the Processed Orders ta
7. and comments E Processed Orders Search criteria Viewbelween v 2008113 and 2008 11 20 gt Last name d tevens gt v Search V Show only exact matches ig Pending Orders 6 1 6 Deleting an Order Slide A selected order slide can be deleted by clicking Delete Select multiple orders slides using CTRL or SHIFT When all slides in an order have been deleted the order is automatically deleted Note Signed slides cannot be deleted However deleting an order deletes all slides in the order signed or not Only Administrators can delete signed orders 6 1 7 Exporting Orders From the Database View you may export signed orders from the current database to another one Create an Export database or a CellaVision Competency Software database before you begin to export In the Tools menu select Settings and then the Database tab For more information see 9 1 1 Creating a New Database Exporting to an Export database 1 Select one or several orders 2 Click Export 3 Select an Export database from the list 4 Check Delete orders after export If not the order will remain in the database you exported from 5 Click Export Cont d User s Manual 63 CELLAVISION 6 CellaVision M DM1200 Cont d Exporting to a CellaVision Competency Software database 1 Select one or several orders 2 Click Export 3 Select a CellaVision Competency Software database 4 Click Export Note tha
8. s Manual 129 TN CellaVision M DM1200 Firefighting Procedures Fire and explosion hazard Flash point gt 300 F 149 C Upper explosive limit N A Lower explosive limit N A Fire fighting media Carbon dioxide Foam dry chemical and waterfog Fire response procedures Fire fighters must use self contained breathing apparatus Unusual fire and explosion hazards Containers should be kept cool in the event of fire 130 User s Manual e CELLAVISION Appendix B Storage and Handling Storage The system shall be stored within the following climate condition 10 C to 40 C 50 F to 104 F at a maximum relative humidity of 80 with no condensation allowed Transporting the System The system shall be packaged transported and unpacked by CellaVision authorized personnel carrier only We recommend saving the package for possible future transports Disposal Information Magazines As combustible plastics PC ABS The system Please contact your distributor User s Manual 131 NN CellaVision M DM1200 132 User s Manual CELLAVISION 6 CellaVision DM1200 Appendix C Buttons and Indicators If you place the mouse pointer over the buttons or indicators you can view tool tips in the Status bar bottom left hand corner Buttons e CellaVision DM software System Control View F4 Database View F5 Verification View F6 Report View F7 Close Order and Slide Order Data Enable
9. 00 Fax 46 0 46 286 44 70 info cellavision com www cellavision com
10. 04 12 04 Open V w Order 100 580421 3553 John Doe 2006 09 04 13 04 Slide T Order 110 80421 3535 Kent Kitoy 2006 09 04 14 04 WA MT order 120 6806124092 Linda Lindford 2006 09 04 15 04 WA l MT Order30 amp D 79072540822 John Ericson Add to Worklist Esport pen Orders locked by another Slides in user selected order Protect Unprotect Selected slide Data for selected slide An order slide opened by another operator on a CellaVision Remote Review CellaVision DM is written in red text In the Order data and Slide data panels Locked by indicates who has opened the order slide the person logged on to Windows and on which computer 58 User s Manual CELLAVISION 6 CellaVision DM1200 Order List The Order List displays an overview of the orders Click on the column headers to sort the list The date in column Analyzed corresponds to the processing date for the last slide in an order 6 1 1 A EID OrderlD Patient 1D__ FirstName LastName _ Signed by Analyzed Pas fee em MT Oderio 700624 1234 Adam ees 2006 09 04 04 04 v MT Order 20 50321 4321 Ben Benson 2006 09 04 05 04 A B MT Order 30 750211 4321 Claudia Crawford 2006 09 04 06 04 1 MT Order 40 45021 8888 Doris Davidson 2006 09 04 07 04 y MT Order 50 amp D 611211 8888 Eric Ericson 2006 09 04 08 04 4 BW MT Order 60 511211 3553 Felicia Foster 2006 09 04 03 04 4
11. 4 1 3 E mail You may send cell images by e mail 1 Select select for e mail using the right click menu 2 Select Tools Send E mail and the New E mail dialog appears Number of cells included in e mail 1 E mail from CellavisionB loodDifferentialS oftware Send e mail to doctor hospital Subject Mai from CellaVision DM System with cell images Message For default values see E mail tab in Settings 3 If desired change receiver address 4 If desired add Subject and Message 5 Click Send Note You may only send cells from one slide in each e mail Note No patient data is sent in the e mail 38 User s Manual CELLAVISION CellaVision M DM1200 4 1 4 Copying Images to Disk You may copy selected images to disk Select cells for copying 2 In the right click menu select Copy images to disk and the Copy images to disk dialog appears 3 Specify the destination path where you want the images to be saved 4 Click OK Note The directory you specify in the destination path must exist 4 2 Red Blood Cell Characterization The RBC overview image corresponds to the area of 8 microscopic high power fields HPF 100x objective and a 22mm ocular See 9 5 Adjusting RBC Precharacterization Settings g wc EEE ec 5 pur ML sesie EE s Ce of So 0 9 59996079 o 59 5950 9 92 eo 2 Repot al as 0 nomal Po oco Oe oo 9 97 0o 29 9 9o oR Codes O Qucm ve o o9 Boe 2059 LIN o
12. Close A compressed database will occupy less space on the hard disk The performance might also be improved Note It can take a long time to compress a database If the database is gt 10 GB in size it can take some hours The process reguires free disk space twice the size of the database to be compressed Note During compression no client may be connected to the database This means that you cannot compress the database you are currently logged on to 86 User s Manual CELLAVISION 6 CellaVision M DM1200 9 1 6 Setting User Restrictions Here you set the available database search criteria for the Restricted user Select the desired Restricted user search options 9 2 Adjusting Users Settings V ObserverUser ObserverUser Observer V NormalUser NormalUser User V RestrictedUser Restrictedllser Restricted V amp uthorizedU ser AuthorizedUser Authorized V AdministratorLlser Administrator ser Administrator 7 admin Administrator created by the system Administrator Show inactive users Activate this checkbox if inactive users shall be shown in the list New Add a new user see dialog below Edit Edit user information see dialog below Delete Delete selected users Cont d User s Manual 87 UM O CellaVision M DM1200 Cont d There are 5 levels of user authorization Observer Only allowed to view images and results User Can reclassify and recharacterize WBC RBC and PL
13. be GOR Ee 76 T2 Pending OB 24 deve GREE DEG Ed EYDAU CEP 76 7 4 Customizing the System LLV FI RF FF uu 78 7 4 1 Adjusting Database UsersandLanguag8 FF Y uu 78 7 4 2 Adjusting Default Scan ATB cys is eurem OP ye YG Y WG Ve be dL YG ews 78 7 4 3 Adjusting Analysis usa ce 2 eto do pic e ER O OA Ric WR ARAD bb o 79 7 4 4 Adjusting Autodelel amp RR RR RR x RR RR RR ERRARE YS 80 8 SYSTEM INFORMATION 81 9 CUSTOMIZING THE SYSTEM 83 9 1 Adjusting Database Settings add eYa does eee YN YN bx Yu den paca dica CS 84 9 1 1 CreatingaNewDatabas8 FL RL Y eee 85 9 1 2 Deleting a Database sse o ROED Re Ra ck RR RR RR 86 9 1 3 Creating a New Database Connection FFY ug 86 9 1 4 Deleting a Database Connection FF a 86 9 1 5 Compressing a Databas amp FF uu 86 9 1 6 Setting User Restrictions VY YF FY ua 87 9 2 Adjusting Users Settings tanen GY DDA ANG GDG UO pages 87 9 3 Adjusting Analysis Settings ise ERR RETERARESYRPRCREYEEFREREEZREREESE XO 89 9 4 Adjusting Reclassification Settings liliis 90 9 5 Adjusting RBC Precharacterization SettingS Y FF eee 91 9 5 Adjusting PETI Seung cs i xe eurem RECS ed HEC bac DDU R can 92 9 7 Adjusting Report Sian Sel iius dod epe de E ER RD RO poco Ro DROL 93 9 8 Adjusting Standard Comments Settings YFF eee 94 9 9 Adjusting Reference Cells Se
14. class for cells and objects which the system has preclassified with a low confidence level Cont d 10 User s Manual CELLAVISION CellaVision DM1200 Cont d WBC Reclassification for Peripheral Blood Besides the cell classes mentioned above the operator can reclassify cells into the following classes Immature eosinophils Immature basophils Promonocytes Prolymphocytes Large granular lymphocytes Hairy cells S zary cells Other Megakaryocytes Not classed and 15 user defined cell classes Other should be used for cells which the operator identifies as a WBC but of a type other than those listed WBCs put here will be included in the differential count Not classed should be used for cells and objects which the operator cannot identify and wants to exclude from the differential count RBC Precharacterization The system precharacterizes the following RBC morphology characteristics in an overview image Polychromasia Hypochromasia Anisocytosis Microcytosis Macrocytosis and Poikilocytosis RBC characterization The operator can characterize to Target cells Schistocytosis Helmet cells Sickle cells Spherocytosis Elliptocytosis Ovalocytosis Tear drop cells Stomatocytosis Acanthocytosis Echinocytosis Howell Jolly bodies Pappenheimer bodies Basophilic stippling Parasites and 10 user defined characteristics Platelet Estimation The operator counts or estimates platelets in an overview image Sa
15. concentration Calculated estimate 178 x10e3 L C Calculated level Increased C Manual level Increased z 2 If you wish to report the PLT results calculated from the number of PLTs per HPF you have two choices Select Calculated estimate to report a concentration The estimate is calculated as Average PLTs HPF value x PLT estimate factor Select Calculated level to report one of four levels Significantly decreased Decreased Normal or Increased 3 If you wish to override the calculated PLT results select Manual level and choose one of the four levels Important You have to determine your own PLT estimate factor and enter it in PLT settings By default it is set to 0 Note In the calculations several decimals are used The presented results are truncated to 1 decimal User s Manual 45 CELLAVISION o CellaVision M DM1200 4 3 2 Estimating the Platelet Concentration Level The PLT concentration level can be estimated by setting it to four levels Significantly decreased Decreased Normal or Increased directly from viewing the image PLT count Grid size 3 x3 0 88 HPF square Image overview Entry field Concentration level Increased m Use the entry fields to view all parts of the overview image 2 Select Concentration level 4 3 3 Excluding the Platelet Analysis Click Exclude PLT Analysis to exclude PLT analysis results from the slide 4 4 Comme
16. deionized water Store at 4 C to 8 C d Buffer working solution pH 6 8 Combine 127 ml KH2PO4 stock solution with 123 ml Na2HPO4 stock solution ad 5000 ml deionized water Adjust to pH 6 8 Store at 4 C to 8 C Durable for a month at 4 C e Giemsa stain stock solution Merck M9204 Azur eosine methylenblue solution for microscopy contains methanol Store at 15 C to 25 C Durable for several months if kept in a dark bottle f Giemsa working solution Dilute 1 part Giemsa stock solution with 19 parts buffer work solution Stable for 8 hours Cont d User s Manual 147 CELLAVISION 6 CellaVision M DM1200 Cont d Wright Stain Note Differences in staining results may be cause by alterations in pH reagents etc Local adjustments may be needed to achieve best results Be aware of pH variations in water Alternative 1 Sysmex SMS SP 100 slide stainer Solution Reaction time 1 Wright stain 2 min 2 Wright stain diluted 1 10 with phosphate buffer 7 min pH 6 8 3 Rinse in deionized water 30 sec Alternative 2 Beckman Coulter GENS slide stainer Solution Reaction time 1 Methanol 30 sec 2 TruColor Wright stain 2 min 30 sec 3 TruColor Wright stain diluted 1 6 with phosphate 6 min 30 sec buffer pH 7 2 4 Rinse in phosphate buffer pH 7 2 1 min 15 sec 5 Rinse in deionized water 45 sec Wright stain alternative 2 can also be used for manua
17. do 9o Fee eo A oo oo g od 0123 A o Poo 0003 eS No eo on i e Bhd 26000 11 pd O90 o 00 Oa Oo c o e go 39 o So d ES 0 000 u 0220 0 000 o 2 000 9o Soe 03909 600 00999 Anisocytosis 06000 45 coco ow 00o 00 oo o9 oo 94 iC T 9 90958 o ooo 208 c ies 06000 45 4 Se Op NL ad eo go Vo 3 ein ec 9 Maerocytosis 18000 ss 1108 0 90 99 pp to 9 ego 906 09 099 cc 000 Poikilocyosis 00000 13 09 9 9 o s 99 99 95 2 Loses yo O0c e9 50005 o Q9 Tangata 0000 006 oo 9 099 OS 99 6 amp e 90099e 9 co 90900 Schistocytosis oooo 0 0500009 coe eoe o oo 2 009 6 vi Se c0 Helmet cel 0000 Po 9 QS G Bp poo exeo Mo 900695 3 0998 Sickle cell OOOO s9go Q o 9 520 ceo 08 o o Qe 2 Spherocytosis 0000 P 0o 9 o C099 0 s o X 0999 9 e eo 909 S 990 e Elliptocytosis 0000 505 e Qr 00 88 o0 9 0 go 40908595 se o yn of Ovelocytosio 0000 oo 009 209 0 Gg 93 Og 9o 9090 Odg SOF Tear drop cells oooo o e o o 999 8 ove 202 9 to Us 0999 og oat od a 5000 Polpa 9909 69 e9 89 ae od oco 97o 00 9 96 o8 Acenthocytosis OOOO o 0 009 oS o 88 9500 o 909 gore ooo o Coe e Echinocytosis OOOO o o 9009 90 o 09 oo Oo 2 99 9o oe ces o 6 Howell lol oooo o SS 9 90d P oc Os cad 999 0 0 09e 0 oo Or 99 9 ic 0000 S 200 0 98 09 SON op 94 Lo o SaPo8 9 0590 8o dopa 09000 9 9 d 99 00 0 0 000 o so VO NEC MUNERE hk TOR be ph RE pes 9 9 donee o ke oos eos o1 Sog 090 a o 9 o o o DEC ACIE ALLE CLOS Os e0
18. double clicking or click Open Remove slides by clicking Remove Note that signed and closed slides are automatically removed Note Show or hide the Worklist by pressing CTRL W on the keyboard Adding Slides to the Worklist Add slides manually or automatically A Manually 1 Select one or more orders 2 Click Add to worklist All slides belonging to the order s are added Cont d User s Manual 65 MC CellaVision M DM1200 Cont d B Automatically 1 Slides are added when the system finishes processing them This option is set in Analysis in Settings 2 When you open an order all slides belonging to it are added Note The Worklist is emptied when the program is closed 6 2 Database Pending Orders The tab Pending Orders contains a list of all pending orders A pending order is an order that has been manually added to the database but not yet processed This is useful when you do not have a connection to the LIS Note When an order has been processed it will be removed from the Pending Orders list and added to the Processed Orders list E Processed Orders i Pending Orders F OiderlD PaienlD Frame LastName Bith date Nbr of cell ing I MT Order 400 560421 2234 Michelle Lawson 1856 04 21 2001 02 12 Dr Spock MT Order 380 580124 2334 Margret Clark 1968 01 24 1997 10 30 Ester MT Order 380 560121 2234 Eddie Levinson 1956 01 21 125 2001 02 12 Dr Spock MT Order 370 680224 2334 Mike Elson 1968 02 24
19. lineage spec Lymphocyte variant form Plasma cell Large granular lymphocyte Hairy cell S zary cell Other Total 3B 1 15m 100 Pen icon Indicates Cell class comments Tick mark All images have been viewed Blue Contains at least one reclassified cell or object Non WBC Erythroblast NRBC Giantthrombocyte Thrombocyte aggregation Megakaryocyte Smudge cell Artefact Count 1m eB 5H Green Contains no reclassified cells or 2 objects Not classed User s Manual 33 CELLAVISION 6 CellaVision M DM1200 4 1 1 Customizing Views Galleries WBCS are presented by class in galleries The main gallery is always shown together with up to two additional galleries Click WBC Galleries to change the number of galleries E pg pa WBC Galleries Left and right clicking in the WBC and Non WBCs panels also changes cell class in the main and the 2nd gallery respectively You can also select cell class in the drop down list The system keeps track of all WBCs viewed by the operator A tick mark appears when all WBCs of a cell class have been displayed It is not possible to sign the slide unless all cell classes have been viewed Reference Cells A library of reference cells for different cell classes is provided with the system These cells are marked with a CellaVision logo The main gallery always displays WBCs from the slide while the other
20. nucleus Vacuolization Cleft nuclei General RBC slide comment 1 2 3 4 5 B 7 8 3 1 Include code in comment If activated the code is written together with the standard comment Always show expanded If activated the standard comments are visible by default when the comment dialog is opened Cont d 94 User s Manual CELLAVISION 6 CellaVision M DM1200 Cont d Adding a New Standard Comment 1 Click New 2 The Standard Comments Editor appears Standard Comment Editor 3 Enter Code Comment type and Comment Standard comments of type WBC RBC or PLT will only be available in the respective tab General comments are always accessible 4 Click OK Deleting a Standard Comment 1 Select the standard comment in the list 2 Click Delete Modifying a Standard Comment 1 Select the standard comment in the list 2 Click Modify and the Standard Comments Editor appears 3 Edit Code Comment type and Comment 4 Click OK User s Manual 95 UM T CellaVision M DM1200 9 9 Adjusting Reference Cells Settings Click on a cell class to see a list of all Custom reference cells belonging to it Click on a specific cell to display it in the Preview window Metamyelocyte Immature eosinophil Immature basophil Promonocyte Prolymphocyte Blast no lineage spec Lymphocyte variant form Plasma cell Large UM lymphocyte Erythroblast NRBC Giant thrombocyte Thromboc
21. results and exclude slides from the reported result in the Report View You can also e Sign or cancel an order Send order data to the LIS Write a general comment on the order Preview the report by clicking on the Report Preview tab User s Manual 51 UM O CellaVision M DM1200 5 1 Merging Slides Compile analysis results for the whole order based on one or several slides in the Slide Merge tab You can also view all comments associated with an order Result Panel m Here you see the results of each slide in the order In the Reported Result column you see the summarized results An unsigned slide has a Slide ID written together with slide number in cerise color Cont d 52 User s Manual CELLAVISION CellaVision DM1200 Cont d Including and Excluding Slides in from the Reported Result Signed slides are automatically included in the reported result To include or exclude a slide activate deactivate the checkbox next to the Slide ID When excluding slides a dialog is shown where you may write a comment explaining the exclusion Note You can only include signed slides Note You should not merge slides if one or more slides have a confirm cell counter result flag If you do the calculated result in the reported result column will be displayed incorrectly Changing the Reported Result It is possible to change the RBC results and the PLT concentration for the order if reported a
22. show reference cells when checkbox Reference cells is activated The main gallery has shortcuts to display reference cells in the other galleries Click Ref cells in Gallery 2 and the 2nd gallery will display reference cells of the cell class selected in the main gallery 6 Indicates reference cell provided with the system You may expand this library by saving WBCs from processed slides as custom reference cells 1 Right click on the WBC 2 Select Save as custom ref cell in the menu 3 Restart the program The WBC will be displayed at the top of the 2nd or 3rd galleries followed by the reference cells provided with the system If you want to organize your custom reference cells see 9 9 Adjusting Reference Cells Settings Cont d 34 User s Manual CELLAVISION 6 CellaVision M DM1200 Cont d Cell Class Comments Click Comment in the galleries to add a cell class comment A pen icon will appear to the right in the WBC and Non WBCs panels Click this icon to view edit or add more comments see 4 4 Comments WBC Full Screen View Click WBC Full Screen View to display all WBCs sorted by class WBC Full Screen View Note Cell classes that have been fully displayed in WBC Full Screen View will also be tick marked Adjusting Image Color and Brightness Click Color Brightness to display the Jmage adjustment dialog m Color Brightness Color sliders Image Adjustment Reset
23. slide barcode labels Slide magazines Replacement pack with new slide magazines for peripheral blood orange CellaVision Immersion oil pack CellaVision Immersion oil pack are supplied in packages of two immersion oil pack May not be available on all markets CELLAVISION CellaVision DM1200 1 INTRODUCTION 7 1 1 About this User s Manual 0 00 0 eee 7 1 1 1 Warnings and Precautions se chs RE Rex RR REOR eee SR baw REA E oe 7 1 2 Intended Use of CellaVision DM1200 Y FF eee 9 1 2 1 Peripheral Blood Application sess sent eves seed YRFA vere eee bee EXE 9 1 3 General Description of CellaVision DM1200 eee 10 1 3 1 Peripheral Blood Application 0 000 eee 10 1 4 Components and Mechanical Operation YY FYR eee 12 1 4 1 Major Paris of the SySIBITI 2 ea dd ep RU ERR DOR OR GER ako RD oes UO 12 1 4 2 Control Unit Wiring us ica cen RR RC RP RR CR RU Go Y RG 15 2 OPERATING PROCEDURES 17 2 1 Starting ING SYSIOM 414 0045 d pe e eS KL qr ea YDD YU GU Y Y aan 17 2 2 System Control ViEW a dae decor RE RUE phu BORDURE paucae Cb PR E publ eee po dd 18 2 3 Slides and Magazines isses eR Rat RR DER RR eee OR ARRA AUR RR 19 2 0 1 Barcodes sss iex RR pU uw eR ERR ER ER ER C E Ed d S 19 24 Loading Slides into a Magazine 4 424 esse obe DDR ERR RE ERE RE Ee ERE ER 20 2 5 Processing Slides e su EE RE GY Eb GDG DE YG EDDU ER EU 21 2 5 1 Adding Ma
24. the mouse pointer in either zoom mode or scroll mode over the ruler depressing the left mouse button and then by dragging it to its desired placement The ruler will be placed back in the bottom right corner whenever the view is changed The ruler shape can be toggled between the default cross shape and a line shape by double clicking on it The shape will not change back to the cross when shifting views A thin window frame appears around the ruler when the mouse pointer is moved over it to indicate that the ruler can be moved or have its shape changed 40 User s Manual CELLAVISION CellaVision DM1200 4 2 1 Customizing the Red Blood Cell Overview Image Change the magnification of the image by using these buttons Zoom Out a Entire RBC Image Shows the entire RBC image Navigate the image by switching between different control modes The mouse pointer changes accordingly Qt Zoom Mode Hold left mouse button down and zoom in out by moving the mouse pointer up down Scroll Mode Hold left mouse button down and scroll in any direction using the mouse Note You may also double click in the RBC image to enlarge a limited area and use the wheel button to zoom in out User s Manual A1 CELLAVISION 6 CellaVision M DM1200 4 2 2 Characterizing Red Blood Cell Morphology There are two ways to report the RBC result C Use characterization a Report all as normal 1 Select radio button R
25. the program shall prompt the user with a warning message Archive Media Select your archive media CD Archiving Network path or local drive Archived Images The images are divided into groups Use the radio buttons to archive either a certain number of images from each cell class in a group or all available images or no images at all from a group Cont d User s Manual 99 TM CellaVision M DM1200 Cont d PB Cell images Overview images RBC and PLT Normal cells Segmented neutrophils Monocytes Basophils Lymphocytes Eosinophils Abnormal cells Band neutrophils Lymphocytes variant forms Prolymphocytes Promyelocytes Plasma cells Large granular lymphocytes Myelocytes Immature eosinophils Hairy cells Metamyelocytes Immature basophils S zary cells Blast cells Promonocytes User defined WBCs Non WBCs Other Giant thrombocyte Smudge cell Not classed Thrombocyte aggregation Artefacts Erythroblast NRBC Megakaryocyte User defined Non WBCs 100 User s Manual CELLAVISION CellaVision DM1200 Archiving Recommendations It is recommended to keep the size of the database below 20 GB for optimal database performance Always combine archiving with a backup of the database The database contains the path to the archived images If a hard disk crash occurs it will not be possible to restore the archived images unless a backup exists Note Select Compress Database to view the database size s
26. 0 Ta O 009900999 09 ego 99099 58 513 4 oce e 9o lt 500 29 0089 S 0008 9 Qo 9905 9 9 c A wo o o y Number of ABC s used for precharacterization os x 200 olo S P9 efe O9 S Exclude REC Analysis o Sero o eo c s oee RBC comment Bex E AS p haces 4 8 jj OO P The RBC panel is used for characterization of the RBC morphology All morphologies handled by the system are listed Morphologies pre characterized by the system are marked with a small dot Cont d User s Manual 39 CELLAVISION 6 CellaVision M DM1200 Cont d The columns labeled 0 to 3 grade the morphology characteristics Normal Green dot in column 0 indicates a normal level Slight Red dot in column 1 indicates that the morphology is present at a low level Madam Red dots in column 1 2 indicate that the morphology is present at a moderate level Marked Red dots in column 1 3 indicate that the morphology is present at a high level The rightmost column shows the percentage of RBCs in the overview image with the characteristic in question If the precharacterization is overridden by manual characterization the percentage is shown dimmed In the bottom right hand corner of the RBC image there is a cross shaped ruler that represents 14 um across As the image magnification is increased numbers will appear at the ends of the ruler to indicate the scale in micrometers The ruler can be moved around the RBC image by moving
27. 03 MC CellaVision M DM1200 10 1 3 Clean Bottom Plate Pull out the bottom plate and wipe clean any immersion oil 104 User s Manual CELLAVISION 6 CellaVision M DM1200 10 2 Preventive Maintenance No preventive maintenance by the user is necessary Preventive maintenance is to be performed by CellaVision authorized personnel 10 3 Remedial Maintenance 10 3 1 Change Immersion Oil Pack Change the oil pack following the intructions in the animated tutorial Open the animatad tutorial goto Help Tutorial Click Change oil pack WARNING The oil may cause sensitization by skin contact We recommend using gloves Caution Use only immersion oil recommended by CellaVision AB see Appendix A System Specification Change Oil Pack a Open the hood b Place clip on hose c Push down on the oil hose connection and pull out the hose Cont d User s Manual 105 TM CellaVision M DM1200 Cont d d Change the oil pack and connect the hose e Remove the clip from the hose f Goto Maintenance Oil g Click Reset Oil Drop Counter Note If the oil pack and all hoses are emtpy Click Prime Oil before Reseting oil drop counter 106 User s Manual CELLAVISION CellaVision DM1200 10 4 Database Performance 10 4 1 To maintain a high database performance it is very important to control the size of the database It is also recommended to restart the syste
28. 0x and 0 5x magnification which combined yields images with 5x 10x 50x or 100x magnification Digital Color Camera The camera is a high quality progressive scan CCD color camera for maximum image quality and high speed image acquisition Immersion Oil Unit The unit automatically applies drops of immersion oil to a slide An optical drop counter controls the procedure The oil pack contains 150 ml Robot Gripper Unit The robot gripper unit enables fully automated focusing and XY positioning of the slide during process It transports a slide from a magazine and back again after the slide has been processed Barcode Reader The barcode reader scans the barcode of both the slide and the magazine For maximum safety the barcode of the slide is scanned before processing See Appendix A System Specification for more information about barcodes Magazine The magazine can be loaded with up to 12 slides clipped round corner slides Control Unit The control unit controls motors sensors oil applying and illumination It functions as a slave computer to the PC via an unshared 100 Mbit Ethernet connection Casing The casing comprises a metal cabinet and the main hood 14 User s Manual CELLAVISION 6 1 4 2 Control Unit Wiring The image shows intenal cable connections to the CCU BARCODE READER CASING o User s Manual 15 NN Cell
29. 1 Loses J i Brightness Color sliders Changes the color composition of the image Brightness Adjusts the brightness of the image Reset Restores original settings Save Stores individual settings Load Gets individual settings Cont d User s Manual 35 CELLAVISION 6 CellaVision M DM1200 Cont d Individual settings can also be switched using 7oggle Color Brightness Toggle Color Brightness Adjusting Magnification Click Zoom In or Zoom Out to change the magnification in all galleries and in the WBC Full Screen View Note You may also double click on a WBC to enlarge it and use the wheel button to zoom in out 4 1 2 Reclassifying White Blood Cells Reclassify WBCs by dragging and dropping them from one gallery to another Place cursor over the cell image 2 Click and hold down left mouse button 3 Move cursor to the destination gallery then release button You may also drag and drop cells to the WBC and Non WBCs panels Press Ctrl or Shift to select multiple cells Reclassified cells always appear at the top of the gallery A cell class containing at least one reclassified cell is marked blue in the WBC and Non WBCs panels If a cell is reclassified to its own cell class it is considered reclassified and moved to the top of the gallery Note You can never reclassify WBCs on a signed slide Cont d 36 User s Manual CELLAVISION CellaVision DM1200 Cont d Spl
30. 150 1994 10 30 Sister MT Order 360 480624 2234 Sid Fernwilter 1948 06 24 200 1999 12 31 Ester MT Order 350 560421 2234 Clara Spielberg 1956 04 21 2001 02 12 Dr Spock MT Order 340 580124 2334 Louise Clark 1968 01 24 1997 10 30 Ester MT Order 330 5601 21 2234 Billy Hutchinson 1956 01 21 100 2001 02 12 Dr Spock MT Order 320 680224 2334 Mike Carlson 1968 02 24 100 1994 10 30 Sister MT Order 310 780624 2234 Nicky Rooney 1978 06 24 100 1999 12 31 Ester Loa oem Dott STAT mark Empty field Not a STAT order Order is marked as a STAT order Order type Empty field Peripheral blood order 1 Click Add to add a new pending order 2 Double click on a pending order or click Edit to view edit the Order Data 66 User s Manual e CELLAVISION o 6 2 1 Peripheral Blood Pending Orders Dialog Order Data EN MT Order 350 560421 2234 m pud 7 1956 04 21 ei o 6 3 Archiving Data Initially make sure valid archiving settings have been specified Refer to9 11 Adjusting Archiving Settings for details Select Tools and Archive The Archiving Guide will appear explaining the procedure on how to archive data User s Manual 67 EEF Si O CellaVision M DM1200 6 4 UsageLog The system continuously saves data of processed slides and stores it in a Usage Log To view the log select Tools and View Log The log consists of two pages Statistics and Specification 6 4 1 Statistics xl
31. 8 67 other Manual correction of nucleated cells found 5 ee Manual correction Total ber of leated cells missed in all th WBCs NBBCs missed 1 otal number of nucleated cells missed in all the images E Percentage of how many nucleated cells were T found compared to the actual number of at nucleated cells on the images Messages about faulty slide analysis etc Print Result The result should be compared to the laboratory s own established limits Performance characteristics when using standardized staining and smear preparation procedures are presented in Appendix A System Specification Ratio of WBCs NRBCs found 99 5 If any of the requirements regarding the number of nucleated cells or non nucleated cells are not fulfilled an error message will be shown in the Total result panel The cell location results can be printed by clicking the Print Result button Deletion of Cell Location Slides Slides older than five days are automatically deleted at program startup logon 28 User s Manual CELLAVISION CellaVision DM1200 3 2 Self Tests The system performs self tests during startup of the software and at certain points during the operation of the system When the software starts the system is checked before the operator can start analyses During this phase both the hardware and the software components are tested for anomalies as well as various requirements for the operat
32. A Ec ae mee hg E hw RACER ACH CR RR CR 116 128 25 Barcode Problems sss o Vh rao io nd ex PET REIR UO DU ugs 118 12 2 7 Staining PIODIGITIS s sack ate 9 UR RH roe CRI Roe EU ued Coo obo es 119 12 2 8 Gripper Service osos re RW Sead FEAR OR dee bea be YU RR CR 120 13 CELLAVISIONG SOFTWARE 121 13 1 CellaVision amp Remote Review Software YYY Y ug 121 13 1 1 Working with Orders Slides 2 exe Pee DU ERG PAPER 8643 O DEUD 122 13 2 CellaVision Competency Softwar amp YYYY FF Lg 122 APPENDIX A SYSTEM SPECIFICATION 123 APPENDIX B STORAGE AND HANDLING 131 APPENDIX C BUTTONS AND INDICATORS 133 APPENDIX D RECOMMENDED WORKFLOW 135 Workflow Single Slides Confirm Cell Counter Results 136 Workflow Duplicate Slides FF FFF ee eee 137 APPENDIX E USER AUTHORIZATION LEVELS 139 APPENDIX F DETERMINING THE PLATELET ESTIMATE FACTOR 141 4 User s Manual CELLAVISION CellaVision DM1200 APPENDIX G SLIDE PREPARATION GUIDELINES 143 Slide Preparation for Peripheral Blood nnna nannaa aaa 143 Example Smears so 5 05652 ai Ere eles KR cop tes banks pa Us ca OR e RAN 145 APPENDIX H GLOSSARY 151 User s Manual 5 CELLAVISION 6 CellaVision M DM1200 Preface CellaVision is a trademark of CellaVision AB All other trademarks used in this document are property of their respective owners No part of this document or the products it descr
33. CellaVision DM1200 CellaVision Peripheral Blood Application USER S MANUAL 3 0 CELLAVISION CellaVision DM1200 User s Manual Optional Software amp Applications CellaVision amp Remote Review Software CellaVision Remote Review Software gives remote users access to analyzed slides and the possibility to reclassify cells and sign slides from another location Smears needing a second opinion can easily be reviewed by a pathologist from within or outside the hospital CellaVision amp Competency Software CellaVision Competency Software is a program for education and competency testing of manual blood cell differentials in laboratories The product allows labs to ensure that staffs are trained to report results consistent with lab policies Accessories amp Consumables Light tower The light tower is an additional product to CellaVision DM1200 The light tower visually indicates system status The light tower also allows an audible alarm to be used HemaPrep6 blood smearing device With HemaPrep the laboratory can standardize the smear quality irrespective of the staff experience By adjusting the speed of the spreader the operator is able to control smear length and thickness Label printer kit Label printer kit enables the laboratory to print it s own slide barcode labels The kit also consists of a hand held scanner for easy handling of labels C 4 QC barcode labels Easy to use preprinted
34. Des Browse C CD archiving ae Images to archive Cell images PB cell images 10 cells class Normal WBCs Abnormal WBCs Non WBCs PB overview images RBC PLT C None All BF overview images Overview images C None 10x C 10x 50x Region of interest images C None Al BF cell images WBCs Non WBCs Note Only images from signed slides can be archived Orders Archiving Strategy The archiving strategy depends on your daily throughput and the need for saving images for future use The autodelete archiving settings are database specific enabling each database to have its own archiving strategy Cont d 98 User s Manual CELLAVISION CellaVision DM1200 Cont d 1 Autodelete Autodelete instead of archive activated Warn when the following two conditions are met 1 The number of signed orders exceeds 300 2 These orders are older than 20 days All signed orders are permanently deleted when the time has expired However autodelete will not begin until the specified number of candidates has been reached Example With the setting above autodelete will not begin until there are 300 orders older than 30 days 2 Archive images Autodelete instead of archive deactivated Keep locally at least Specify the minimum number of days that images from signed slides shall be stored Warn when candidates reach Specify the minimum number of non archived signed orders at which
35. EUS S Slide number 1 neutrophil Sample date 2009 04 03 Ready admin FT9706 CAP NUM 18 User s Manual CELLAVISION CellaVision DM1200 2 3 Slides and Magazines 2 3 1 Barcodes Important Only slides labeled with barcodes can be processed Use only barcode formats appropriate for the system see Appendix A System Specification Magazine ID The barcode on the magazine is the magazine ID When the system has no LIS connection the magazine ID determines the analysis type e Orange magazines with barcodes consisting of prefix PB followed by digits a peripheral blood analysis will be performed The barcode needs to consist of at least 3 digits Note LIS order will override the magazine barcode in determining analysis type Cont d User s Manual 19 CELLAVISION 6 CellaVision M DM1200 Cont d Slide ID The Slide ID is the barcode printed on the slide s label Note The slide ID may not begin with PB When processing a slide the system searches for order data in the following order 1 In the database unsigned orders with the same Order ID 2 In the database pending orders 3 From the LIS 4 Default values defined in the Analysis tab in Settings 2 4 Loading Slides into a Magazine Load the slide into the magazine with the barcode upwards and towards the open end 12 slides can be loaded into a magazine The slide positions in the magazine are numbered 1 12 f
36. ION 6 CellaVision M DM1200 Romanowsky stain An eosin methylenblue solution for staining of blood smears Wright and MGG are examples of different Romanowsky stains Signing Finally confirming analysis results before locking and reporting them Slide ID The barcode number on the slide PID Same as Order ID Slide number A number that uniguely identifies slides within the same order Slide position The position of a slide in the magazine Unidentified Cells and objects which the system cannot preclassify Verification The operator s review of WBC RBC and PLT e g reclassification and confirmation of WBCs WBC White blood cell leukocyte Wright A Romanowsky stain for blood smears Wright Giemsa A Romanowsky stain for blood smears 152 User s Manual CELLAVISION CellaVision DM1200 A Archive status oei Y wd 60 Archiving uie dures TAa Geen OS ares 97 Authorization Levels 139 AONB 24 bcos esa ERR Reg s 21 134 B se 2g gtd epa alain ose a aa s 69 Barcode Y Y YY 19 118 Balcli Saws ss oosakuds biis here RR 22 Blood Films 3 24 RL RR 143 BUtON uus eoo NE NEN RR 133 C Canceling an Order 56 Color and Brightness 35 Comment Panel 3232459 408304050 dr RA 54 Comments 46 60 61 94 Creating a New Database 85 D Database View 31 57 76 133 Default Values ue Go ER EN 23 Deleting a Databas
37. M1200 9 4 Adjusting Reclassification Settings LI LI LI LI LI Forward preclassified cells Select your forwarding criteria using the checkboxes 90 Users Manual e CELLAVISION 9 5 Adjusting RBC Precharacterization Settings Here you adjust the percentage limits for the different levels of the morphology characteristics It is also possible to disable the RBC precharacterization Enable RBC precharacterization RBC limits 1 Slight 2 Moderate 3 Marked 1 Slight 2 Moderate 3 Marked Polychromasia fi 5 25 Anisocytosis e Hypochromasia e 20 Microcytosis e Poikilocytosis 2 15 Macrocytosis G NEN lE 1 Anisocytosis sizes micrometers Microcytosis lt 65 lt Normal lt jas lt Macrocytosis Reset RBC Limits and Anisocytosis size to their default values Reset Note Default values according to O Connor Barbara H A Color Atlas and Instruction Manual of Peripheral Blood Cell Morphology Lippincott Williams amp Wilkins 1984 Enable RBC precharacterization Deactivate this checkbox if RBC precharacterization shall not be performed Manual RBC characterization will still be possible RBC limits The percentage limits used for RBC precharacterization The default values are set according to O Connor Barbara H A Color Atlas and Instruction Manual of Peripheral Blood Cell Morphology Lippincott Williams amp Wilkins 1984 Anisocytosis sizes
38. MT Order 70 700611 3553 Glen Gordon Authoriz 2006 09 04 10 04 Order status Jj Empty field No slide is signed v At least one slide is signed amp Order is signed Ara Order is cancelled STAT mark Empty field Not a STAT order Order is marked as a STAT order LIS status Empty field No data sent or received is Data received ea Waiting to send result a Result is sent W Result send failure g Result is successfully sent Cont d User s Manual 99 TM CellaVision M DM1200 Cont d Process status Empty field All slides in the order have process status OK e Slide with process error in the order D Stopped slide in the order A Slide with process status in the order Archive status ts Empty field Order is unprotected tj Order is protected Order and slides in this order cannot be deleted or archived amp Order is archived Comments d Empty field No comments di Comments exist Order type Empty field Peripheral blood order 60 Users Manual e CELLAVISION 6 6 1 2 Slide List Comments slide and processing status are indicated in the slide list Processing status Slide status Comments ed bi Y v 6 1 2006 08 21 16 35 admin 6 2 2006 08 21 16 36 v 6 3 2006 08 21 16 39 6 5 2006 08 21 16 42 Process status M Y Slide processing OK D Slide processing stopped No result exists A The required numbers of WBCs were not found one of the ordered analys
39. RRU au a 55 Report template 222 ovr RR ON 83 Report VIEW GR YR eres 133 Restricted USB 4 aeris e RD ROS 83 Result Panel ye d e ERU A Y Ni 52 Right click Menu a Sd FED 38 User s Manual 153 UM E CellaVision M DM1200 S Scanning a Slld amp a sux e eRRCA PRA RENS 71 BEICDIES ud adco e dara Ri E ri ho RR ESS 135 Shut down L1s3eekiessrererexuberd aes 24 Signing a Slide i posa EXP o aus 49 Signing an Order ssuun 55 Single slide differentials 135 S de T Loses ke EE 20 Slide Information Dialog 23 Slide Preparation duree eset ex eus 143 Slide scanning unit 12 13 Slide Stat sS 45 0445 UD Gu O 23 Splitting Cells 4 RA tana aes 37 SOW oe ee Ed PN HT 12 Staining Recipes 2 2 e LD 146 Standard Comment 95 sca m P 17 21 134 SUOMBE RY ER 131 System Control La A ys 18 System indicators 18 T Tagging Regions of Interest 74 Jc PRI 18 Troubleshooting 109 V Verification View 31 133 W Warning texts P 23 WBC Attributes cU WD Ag 37 WBC Galleries ccs E a EDR 34 WBC patel uoces wh GYD Fn 31 WOFEHOW sus _ O RR 135 Worklist Lek supere Cutie P ERI DERE 31 Wight RF FU Y I RF HWN 148 154 User s Manual C CellaVision AB Ideon Science Park SE 223 70 Lund Sweden Phone 46 0 46 286 44
40. Set size limits for precharacterization of Anisocytosis Reset Resets all RBC limits and Anisocytosis sizes to their default values Note Poikilocytosis General term used to describe the presence of abnormally shaped RBC User s Manual 91 J CELLAVISION 6 9 6 Adjusting PLT Settings In the PLT tab you set the PLT estimate factor and the limits for the PLT concentration levels You also set the default settings for the grid size PLT count and PLT concentration result It is also possible to enable the option to use manual PLT concentration estimation J Use only manual PLT concentration estimation Number of HPFs 8 per overview image PLT estimate factor ao Defaults for PLT tab Grid size 3 x3 0 89 HPF square PLT count Count PLTs per grid square PLT concentration Calculated estimate Intervals for average PLTs HPF value lt Significantly decreased lt I TE Do e Decreased lt DESEE Normal sme Increased Use only manual PLT concentration estimation When this checkbox is activated PLT estimation is done by estimating the concentration level to Significantly decreased Decreased Normal or Increased No values for the PLT count can be entered and no calculation can be performed This setting takes effect the very first time a slide is opened and can then not be changed for the order PLT estimate factor See Appendix F Determining the Platelet Estimate Factor
41. T but not sign slides Authorized Same as User but can sign slides and orders Restricted Same as Authorized but with restricted database access Administrator Full access to the system The authorization level determines the different functions and features the operator can access see Appendix E User Authorization Levels User Information Users Full Name NEN Password fo Password again fo Email Access level Observe el IV Account Active required field User Login name The checkbox Account Active indicates if the account is active Full Name Full name of the operator Access Level Level of user authorization E mail The operator s e mail address Note When you create a new user the username must be unique and start with a letter a z or A Z The following words are reserved USER SYSTEM SELECT TABLE UPDATE CREATE GET IF IS GRANT IN LOCAL OF ON OR OUT SET TO 88 Users Manual CELLAVISION CellaVision DM1200 9 3 Adjusting Analysis Settings Default values These values are used if the order to analyze is not found in the database or in the LIS Type of order is the analysis to perform Enable LIS Activate this checkbox and restart the program if the LIS will be used Add processed slide to worklist If this checkbox is activated processed slides will automatically be added to the Worklist User s Manual 89 ne CellaVision M D
42. When the last slide in the worklist has been signed the Database View automatically opens 136 User s Manual CELLAVISION CellaVision DM1200 Workflow Duplicate Slides Go to Database View Shift F1 Click Add to worklist Click on the Slide Nbr header in the Worklist x N Select all slides with Slide Select all slides with Slide Nbr 2 Click Remove Nbr 1 Click Remove Double click on the first slide in the Worklist Verify WBC Click RBC tab Perform RBC review Click PLT tab Perform PLT review Click Sign Slide tab Sign the slide When the last slide in the order is signed answer No to question whether to sign order i The next slide in the worklist will automatically be opened When the last slide in the worklist has been signed the Database View automatically opens Double click on the Order you would like to report If all slides are signed the Report View automatically opens If necessary adjust the calculated RBC and PLT results Click Sign Order If you want to make a printout activate the checkbox Print Order The Database View automatically opens User s Manual 137 NN CellaVision M DM1200 138 User s Manual CELLAVISION CellaVision DM1200 Appendix E User Authorization Levels Action Setting Observer User Restricted Authorized Administrator Start slide processing X X X Search in the database on Or
43. YYY FL uu 44 4 3 2 Estimating the Platelet Concentration Level eee eeee 46 4 3 3 Excluding the Platelet Analysis V Y Y uu 46 4 4 SCOMMEMG lt 6 duce acia O O Y O he RD RAT de RR A A EC PF FFR dre n A 46 4 4 1 Adding Comments o Y EID RR FD UR CR ts 47 2 5 Oder Dal oni ooi Reb de o doe qi We hohe FRIFO FFF RNA Dreh Id 48 46 Signing a Slide ML Dd DG MOD Dw O DD YU OD ORDD DAWDD 49 4 6 1 White Blood Cells Red Blood Cells and Platelets 50 5 REPORTING RESULTS 51 SA Merging Slides es eu ade FN GG ee Yn UG ob eM eee Reka Ead 52 5 2 Report PTEVIGW a 9 espe bb tii nta FRO AO CRUD E E Sob RO EROR T pra EU pees ene 55 5 3 Signing an Order result sac peu UG CUD DWR DDU RC RU REG 55 54 Sending an Order to the Ll8 eb Y RR ERR ER RERO ER RR GC RR A 55 55 Canceling am CIn7Zlels ius os oos CN teen oS sb eae FN NC A a 56 6 DATABASE AND ARCHIVING DATA 57 6 1 Database Processed Orders esso ke RREELRRERFEREPRFERRPRREDed GR WOOD eek ee 57 6 1 1 Order lish PPP 59 6 1 2 Slide Listes aegse eat ee ete Oh HR toe lees pee E RA eee ee od 61 6 1 3 Opening an Order Slide ya Gy GW aera be oes eevee Gave heeeaeds 62 6 1 4 Protecting an Order 2 a2 a u quia 2 GWD DD WG cook a een eee Oem Rae ps 62 6 1 5 Searching for an Order Slide 0 00 eee 63 6 1 6 Deleting an Order Slide llle 63 6 1 7 Exponing Ode 2 422461 ANO Yd o YD GDG DU aides 63 6 1 8 Copying Images to DISK v5 e
44. aVision M DM1200 16 User s Manual CELLAVISION CellaVision DM1200 2 Operating Procedures 2 1 Starting the System The CellaVision DM system computer is configured with a Windows policy restricting access to the operating system for the normal user When starting the PC the user will automatically be logged onto Windows and then the CellaVision DM Software logon window will be displayed Start the system as follows 1 Switch on the slide scanning unit 2 Switch on the system computer Wait until the status lamp on the slide scanning unit is flashing or continuously lit see picture in 1 4 1 Major Parts of the System 3 In the Log On dialog type username password and select the desired database The following icons indicate available database types t Processing database a _ Export database CellaVision Competency Software database I Scan database CELLAVISION 6 Username Password Database fe YourDatabase E ces 4 Click OK User s Manual 17 MW CellaVision M DM1200 2 2 System Control View The System Control View shows the ongoing slide processing gives an overview of the preclassification and presents a log of processed slide holders The layout of the System Control View depends on the type of application Click System Control View in the toolbar Information in Toolbar System indicators show oil level hood position For a description of these indicators see A
45. abeled end of the slide Narrow a spreader slide with polished edges at a 30 to 45 degree angle to the smear slide Allow the blood to spread almost over the entire width of the slide Then rapidly and smoothly push the spreader slide to the opposite end of the slide There should be a gradual transition in thickness ending in a squared or straight edge without any grainy streaks troughs ridges holes or bubbles The blood film must be at least 30 mm in length terminating 5 15 mm from the edge Cont d User s Manual 143 CELLAVISION 6 CellaVision M DM1200 Cont d 4 Dry the slide rapidly 5 Stain the slide within one hour i 5 15 mm 33 mm The dot in the image indicates the analysis starting point The analysis proceeds from the starting point towards the thinner part of the smear 144 User s Manual e CELLAVISION CellaVision DM1200 Example Smears The line in the images indicates the analysis starting point Accepted UN uw a NAE an These slides are all prepared according to the specifications and will be accepted in the DM1200 Not Accepted I E NET These slides are not prepared according the specifications and might not be accepted in the DM1200 Cont d User s Manual 145 CELLAVISION 6 CellaVision M DM1200 Cont d Recommended Staining Recipes CellaVision DM is optimized to analyze samples
46. atrix OR Cont d User s Manual 127 CELLAVISION 6 CellaVision M DM1200 Cont d Reguirements Sample ID When using either a DataMatrix or a QR code the only information allowed in the code is a sample ID i e no other data which may be at most 24 characters long but which may be padded with spaces ASCII code 32 No leading spaces are allowed Print guality The printed barcode shall be resistant to immersion oil and be printed with a high contrast ratio between background and the printed barcode If the barcode is printed directly onto a slide it must be printed on a white smooth frosted area of the slide Barcode size The following barcode resolutions have been verified at CellaVision Supported code resolution Code Cell size mil OR 13 DataMatrix 9 Code 39 7 Code 128 7 Codabar NW7 7 Interleave 2 of 5 7 Cont d 128 User s Manual CELLAVISION CellaVision DM1200 Cont d Quiet zone The recommended quiet zone is listed in the table below Vertical quiet zone cells Horizontal quiet zone cells Symbology Top Bottom Top Bottom DataMatrix 1 1 1 1 OR 4 4 4 4 OUIET ZONE a QUIET ZONE QUIET ZONE Barcode position The barcode shall be positioned within the area as illustrated in the figure below Area where the 2D barcode can be printed Where X lt 18mm X gt 6mm Y gt 4mm User
47. b and Worklist the text colors indicate Blue opened order and slide Red order is locked by another user Highlighted in blue or grey selected order or slide Worklist Opened order and slide Selected order Data for selected order Vending Orders Worklist E 6 ed Oder fi Oden s MT Order 40 Search criteria Order data Order ID MT Order 10 1 2 slide s in result n View latest z 20084028 and 006 03 Ww deed by E ad idels i a 2006 09 04 04 04 N S None sj bd jig LIS status Data received Type of order WBC Search fV Show oy exact matches Patient ID 70624 1234 Adam Anderson Gender Male Birth dale 1970 06 24 a EH Order Patientin FistNane LastName Signedby Analyzed _ Ethnic origin Asia y le ill Mr Order 10 70062441234 Adam Anderson 2006 09 04 04 04 ard Neuro clin v ll MT Order 20 6503214321 Ben Benson 2006 09 04 05 04 Pen comment 9 ll MT Order 30 7502114321 Claudia Crawford 2006 09 04 06 04 Migrane MT Order 40 450211 8888 Doris Davidson 2006 09 04 07 04 bu Irder comment v lj MTOder5D D 6112110008 Eic Ericson 2006 09 04 08 04 My aps enak N male v WAD MT ydes 511211 3553 Felicia Foster 2006 09 04 09 04 e d MT fider 70 70611 3553 Glen Gordon Author 2006 09 04 10 04 i a a d MT Grder 80 6506113553 Heny Henyson Authoriz 2006 09 04 11 04 a B Mf Order 30 710411 3553 Isac Irving Authoriz 2006 09
48. cal evaluation on 230 patient samples based on NCCLS standard H 20A Cell class SD 9c Cell class SD 9c Segmented neutrophils 3 8 Basophils 0 7 Band neutrophils 1 6 Lymphocytes 3 4 Eosinophils 1 0 Monocytes 2 0 Limitations Distinctions between band and segmented neutrophils metamyelocytes and myelocytes myelocytes and promyelocytes lymphocyte and lymphocytes variant forms are subjects to variations among individual operators Depending on WBC concentration number of non WBCs and the quality of the smear Cont d 124 User s Manual Cont d Performance Specification Scan Throughput data and disk space reguirements for the scanned slides CELLAVISION CellaVision DM1200 Analysis area size Overview images Processing time Disk space 5x5 mm 10x 1 5 min 10 MB 5x5 mm 10x 50x 12 min 130 MB 10x10 mm 10x 3 min 45 MB 10x10 mm 10x 50x 45 min 500 MB The given values are approximate Processing time and required disk space vary depending on the sample Materials Specification Immersion oil Trak 300 Automated Differential System Immersion Oil Refractive index 1 5150 Viscosity 300 cSt PCB free 150ml oil bag should be sufficient for approximately 3000 analyses CellaVision slide magazines Stain May Gr nwald Giemsa Wright or Wright Giemsa Slide requirements mm glass 75 0 76 0 x 25 0 26 0 x 0 9 1 2 ground edges cli
49. ctive tab Standard comments can be added and edited in Settings see 9 8 Adjusting Standard Comments Settings To clear comments click Erase 4 5 Order Data To edit order data click Order Data in the toolbar A dialog is shown containing information about the open order If no data is received from the LIS it is possible to edit some of the information The Order ID can only be edited if it starts with ER The Order Data dialog can also be accessed by right clicking on an order in the database view If you can t generate barcode labels printer is broken out of printer ribbon or out of labels you can use pre printed labels with the prefix ER available through your local dealer 48 Users Manual CELLAVISION CellaVision DM1200 4 6 Signing a Slide A summary of the WBC RBC and PLT analyses is presented in the Sign Slide tab Ml Sion Slide The signing procedure is as follows 1 Click Sign The Sign Slide dialog appears Sign Slide Database YourDatabase Type Processing User name my UserName Password fV Sign order when signing slide IV Sendto LIS Print order Caes 2 Enter User name and Password 3 Activate deactivate the automatic options Sign order when signing slide Send to LIS and Print order 4 Click OK Note If the slide is part of a multi slide order all slides in the order must be signed before the operator is given the option to sign the or
50. der Note Slide data cannot be changed after signing Comments may still be added If all ordered analyses have been viewed when the Sign Slide tab is selected the Sign Slide dialog will automatically appear Default values for the Sign Slide dialog are set in Settings under Report Sign The option to send results to the LIS is activated in Analysis in settings When the last slide is signed in a multi slide order the operator has the option to sign the order User s Manual 49 UT CellaVision M DM1200 4 6 1 White Blood Cells Red Blood Cells and Platelets Before signing a slide all ordered analyses must have been viewed by the operator The following conditions must be fulfilled WBC e All cell classes must have been viewed i e there should be tick marks after each cell class No WBCs remain in Unidentified RBC An RBC characterization must be reported or excluded from the analysis Report a characterization by selecting one of the following options C Use characterization OR Click Exclude RBC Analysis PLT A PLT concentration must be reported or excluded from the analysis Report a PLT concentration as described in 4 3 Estimating Platelets OR Click Exclude PLT Analysis 50 User s Manual CELLAVISION CellaVision DM1200 5 Reporting Results For detailed information on settings see 9 7 Adjusting Report Sign Settings Click Report View in the toolbar Compare slide
51. der ID Also see section 12 2 5 Cell Location Problems for LIS errors 115 CELLAVISION 6 CellaVision M DM1200 12 2 5 Cell Location Problems Cell Location Problems Peripheral Blood Problem Too many non nucleated cells Cause Too many smudge cells Action Run another slide with less smudge cells Verify smear preparation process Too many artefacts Increase wash step Filter the stain Ensure that the stain has not exceeded the expiration date Cells misclassified as non nucleated cells Run another slide and if the problem remains prepare new staining solutions Too few nucleated cells located Nearly all boxes are far from the cells Low WBC count Prepare a new slide with a higher WBC count Too short smear Prepare longer smears Poor smear quality Stage or calibration error Verify smear preparation procedure Contact your local vendor s technical support Too many missed cells Too high WBC count Do not process slides with a WBC count that exceeds 100x109 I Poor staining Verify staining procedure Long processing time Low WBC count Prepare a new slide with a higher WBC count Poor staining Verify staining procedure 116 User s Manual 12 2 6 Barcode Problems CELLAVISION CellaVision DM1200 Problem The barcode could not be read Cause Bl
52. der ID and X X X Patient ID Search in the database on other criteria X X Verify and comment WBC RBC and PLT Edit Order data Add edit pending orders X X Sign slides and reports Send results to the LIS X gt lt i lt Xxx Delete unsigned slides orders Delete signed orders Archive Export orders gt lt Protect order from archiving Send images with e mail Export log files Database settings X X PS ss lt l PS PS PS Xx lt lt OK Users settings Analysis settings Default values Add processed slide to X X worklist Enable LIS X WBC reclassification settings RBC precharacterization settings User s Manual 139 CELLAVISION o CellaVision M DM1200 Action Setting Observer User Restricted Authorized Administrator PLT settings Use only manual PLT concentration estimation X X X X Defaults for PLT PLT estimate factor Intervals for average PLTs HPF Report Sign settings gt lt Standard comments settings Reference cells settings X E mail settings lt lt Xx Archiving settings 140 User s Manual CELLAVISION CellaVision DM1200 Appendix F Determining the Platelet Estimate Factor Follow the procedure below to determine the PLT estimate factor 1 Perform automated PLT counts with a cell c
53. der was not found in the LIS The required number of WBCs were not found or one Eneoroplete analysis of the ordered analyses failed Error Texts Invalid slide PID Invalid barcode on the slide Analysis failure Slide processing failed Critical failure A critical error occurred Invalid batch PID Invalid barcode on the magazine Slide Information Dialog Double click on a slide to open the Slide Information dialog Additional information on the processed slide e g the cause of an error is displayed here User s Manual 23 TM CellaVision M DM1200 2 6 Ejecting Magazine The magazine currently being processed can be ejected 1 Click Stop Note Always stop processing before ejecting the magazine 2 Select Eject in Tools menu 2 7 Shutting Down the System Shut down the system as follows 1 Eject and remove the magazine from the system 2 Select Exif in the File menu 3 Switch off the system computer 4 Switch off the slide scanning unit 24 User s Manual e CELLAVISION 3 Quality Control 3 1 Cell Location Performance The Cell location test is used to verify the slide preparation process and the system hardware The cell location performance shall be verified at regular intervals and after changes in staining procedure or staining solutions by running the Cell location test Note Running the test once or twice a day is a recommended interval at the high load laboratory 3 1 1 Cell Loca
54. directory Create a Database Database name Description Home directory JC Program FilesCellavisionsBloodDifferentialN Type of database Processing database Normal C Scan database C Export database CellaVision Competency Software database Database size 20000 MB Free disc space 58284 MB Note Creating a database can take up to 20 minutes coes Creating a database reguires that there is sufficient free disk space A processing database reguires 20 GB a scan database reguires 20 GB an export database requires 1 GB and a CellaVision Competency Software database requires 100 MB Note Having many databases affects system performance negatively Note A database can be created only on your local computer User s Manual 85 CELLAVISION CellaVision DM1200 9 1 2 Deleting a Database 1 Select the database in the list 2 Click Delete 9 1 3 Creating a New Database Connection 1 Click Connect A dialog appears where you may enter Database name Description and Remote computer 2 Click OK A new connection will be shown in the list Note On the remote computer select Help System Information to find out the name of the computer 9 1 4 Deleting a Database Connection 1 Select the connection in the list 2 Click Disconnect 9 1 5 Compressing a Database 1 Select the database in the list 2 Click Compress A dialog appears 3 Click Compress database 4 Click
55. e is connected to both the camera and the system computer Restart the program Critical error No connection with the database Connection with the database has been lost Check network connection Restart the system computer or the computer on which the database resides Critical internal error during cell collection Temporary software error Restart the SSU and the system computer disconnected Critical internal error Temporary Restart the SSU and the system software error computer Camera initialization error The camera Make sure that the camera cable is cable is connected to both the camera and the system computer Restart the program User s Manual CELLAVISION CellaVision DM1200 12 2 3 General Processing Problems Problem WBC images are not centered on the cell but the cell can still be seen Cause System needs calibration or alignment Action Contact your local vendor s technical support Slide status Incomplete analysis Less than the ordered number of cells are collected Too small monolayer found Prepare the slides with longer blood films Artefacts staining precipitates on the slide Make sure that the solutions are changed according to the recommendations in Appendix G Dust on objectives Perform weekly maintenance on the current objective see 10 1 1 Cleanin of Objectives and LED table
56. e isa DUR Y 86 Digital Slides ac AR ON 71 Disposal Information 131 Duplicate Slides 137 E issu PP 38 entry fields a RG RR RD RR 43 BIO texis 2529s RY es 23 Estimating i22 aa n D e RR ERES 43 Excluding the Platelet Analysis 46 Excluding the Red Blood Cell Analysis 42 F Full Screen View 4445 o s 35 G Gall ties I Y RA ER ake 34 grid squares Losses eiue xh pp Rees 43 H high power fields 39 43 l Indicators 133 134 Inserting the slide holder 21 L B EE FEN AEN NU AREE 33 LlSstatus eese 59 115 Loading Slides ia saesosuen d GO OR 20 M Magazine ID a ROL GER Da 19 Magazine Status 3 FR YAR 22 main gallery eue eu og dass ER ede PER 34 Maintenance ou osse copa YG DS 103 May Gr nwald Giemsa 125 Mini Map YF Y Y ee eee 72 73 N Navigating uiid Y dy T3 O ODSCIVE a GC GYD YA DUR 88 Oil Refill ib ene y orien 105 Order SOUS oves hes RP 59 Overview Image a i iY Y 72 P PB DDN PCIE 9 PLI Count A d RerOAXYXVRe Dey EOS 44 PLT estimate Factor seco aes es 45 92 PET Resull Sk RR RR ERR Rx 45 PROCESS HAMS ccce acu eiris e Rd 61 R RBC characterization nu 91 RBC limits 24 04 RW enon bes 91 RBC overview image 39 Reference Cells 34 96 Regions of Interest 75 MORON osos aqui ker n 133 Report Preview i e GY
57. e previous one is closed Message box Wrong Enter correct username and password connection error Caps Lock is Deactivate Caps Lock password correctly activated Message box The database If the database is stored on the system Could not log on to the server could not computer restart the computer and the system Network be reached program Otherwise check the network connection and that the computer that stores the database is turned on Message box Disk space exhausted The database is located on the C drive Move the database to the EN drive Logging on to the system takes time The database server needs to be restarted Restart the system computer or the computer on which the database resides The database is too large Keep the database size at the recommended level see 10 4 Database Performance The database files are fragmented Compress the database see 9 1 5 Compressing a Database CELLAVISION 6 CellaVision M DM1200 110 12 2 2 Error Message List software error Error Message Cause Action Startup error messages The system is not ready Temporary Restart the SSU and the system computer The configuration of the Control Unit is incorrect The Control unit has lost its configuration Contact your local vendor s technical support Communication failure No connection between the SSU and the system comp
58. ed methods see Appendix G Slide Preparation Guidelines General Functionality of the System Receives order information from and sends results to the LIS Locates and presents images of every located cell or object found on the smear Stores images and results in a database Presents an overview image of a user defined area on a slide 1 3 1 Peripheral Blood Application General Functionality Presents an image on a screen of every located cell or object e Organizes and suggests cell classification preclassification for white blood cells Makes it possible to identify confirm or modify reclassification the suggested classification of white blood cells Presents and suggests morphological characteristics precharacterization in an overview image of red blood cells Makes it possible to confirm or modify the precharacterization of red blood cell morphology Presents an overview image and facilitates platelet estimation WBC Preclassification The system preclassifies the following WBC classes Band neutrophils Segmented neutrophils Eosinophils Basophils Lymphocytes Monocytes Promyelocytes Myelocytes Metamyelocytes Blast cells Lymphocytes variant forms and Plasma cells The system preclassifies the following non WBCs Erythroblasts NRBC Giant thrombocytes Thrombocyte aggregations Smudge cells and Artefacts Non WBCs are reported as number of cells or objects 100 WBCs Unidentified is a
59. edges e Clipped or round corners Barcode labeled Frosted end Note Coverslipped slides are supported as long as the total thickness of the slide is within the requirements Note When using cover slips make sure the scanning area is within the cover slip area Note Any type of CellaVision DM1200 magazines can be used for a scan analysis User s Manual 71 CELLAVISION 6 CellaVision M DM1200 7 2 Scan Overview Image Opening a scanned slide leads directly to the Scan Verification View To open a slide see 6 1 3 Opening an Order Slide 7 2 1 Overview The Mini Map shown in the Overview displays the entire sample area To enlarge a specific area of the sample click in the Mini Map The magnified area will be displayed in the large image on the screen On the Mini Map a red rectangle marks the area reguested for enlargement A blue trace marks an already viewed area Mini Map Trace Current position B overen Region of Interest Region Comment 111 Zoom panel Add scan comment Region of interest Navigation buttons panel The enlarged area can either have a 10x zoom or a combined 10x and 50x zoom Using both zoom levels will increase the processing time 72 Users Manual CELLAVISION CellaVision DM1200 7 2 2 Navigating Move efficiently in the Mini Map by using the keyboard arrows or the Navigation Buttons ERa v The image shown in the ri
60. ee 9 1 5 Compressing a Database Hard Disk Space Requirements Peripheral Blood A processed slide with 100 WBCs collected will require about 3 MB of hard disk space RBC and PLT use the same overview image and will require about 1 MB A processed slide with 100 WBCs and RBC and or PLT ordered will require about 4 MB of hard disk space Note Always delete unsigned or failed orders User s Manual 101 MW CellaVision M DM1200 9 12 Adjusting Language Settings In the Language tab you set language for the user interface and manual Language Language for the user interface T he change will take affect when the program is iestarted Language for the online manual English 102 User s Manual CELLAVISION CellaVision DM1200 10 Maintenance Caution Be careful not to bend or put excessive force to any part of the system interior 10 1 Weekly Maintenance 10 1 1 Cleaning of Objectives and LED table Clean the objectives and the LED table folllowing the intructions in the animated tutorial Use lens paper to clean the objectives and the LED table Open the animatad tutorial goto Help Tutorial es Click Clean Important Make sure there is no oil on the low power 10x dry objective Note When needed use Isopropylalcohol to clean the objectives 10 1 2 Delete Unsigned Orders Delete unsigned and failed orders to minimize the size of the database User s Manual 1
61. end Check that it is within acceptable limits Cont d User s Manual 25 TN CellaVision M DM1200 Cont d Examining a Cell Location Slide Select the slide in guestion from the list of cell location slides Cell location slides Slide ID Analyzed QC035 2006 08 22 08 30 Qcoio 2006 08 22 08 25 v BCO03 2006 08 22 08 16 w QC036 2006 08 21 14 19 Q ocw2o 2006 08 21 08 22 Slidestatus Empty Slide has no result not all images have been examined T Slide has a result and no missed nucleated cells all images have been examined r Slide has a result but contains missed nucleated cells all images have been examined Slide error probably due to a failure in slide processing e g o not enough nucleated cells were found Go through each image in the list of overview images and check if they contain any missed nucleated cells Green boxes mark the nucleated cells and blue boxes mark other found objects that are not nucleated cells e g artefacts Missed nucleated cells are those cells not marked with a box in the image Double click in the overview image to magnify the area of interest Black boxes mark the number of cells that were located but not needed for the test indicating that the system has located enough cells for the test and is coming to an end Note On screen cell images are not always presented in the same order as the system is working Cells marked with black boxes may occu
62. eport all as 0 Normal b Use characterization 1 Select radio button Use characterization 2 If you disagree with a pre characterization click the dot that corresponds to your opinion Click Reset to Pre haracterization to restore the pre characterization result done by the system All manual characterization will be lost Note If you want to remove a specific type of morphology from the report deselect the dot by clicking on it 4 2 3 Excluding the Red Blood Cell Analysis Click Exclude RBC Analysis to exclude the RBC analysis results from the slide 42 Users Manual CELLAVISION CellaVision DM1200 4 3 Estimating Platelets The complete PLT overview image same image as for RBC corresponds to the area of 8 microscopic high power fields HPF 100x objective and a 22mm ocular The overview image is divided into 4 9 or 16 sub images grid squares as defined by the grid size The grid size options are 2x2 3x3 and 4x4 The 4x4 grid size gives the largest magnification of the image There are as many entry fields as there are grid squares By clicking Help Lines in the toolbar a grid of lines is drawn over the image to facilitate the counting of PLTs There are two ways or modes to perform the PLT estimation This is determined in PLT settings see 9 6 Adjusting PLT Settings 1 Counting PLIS in the overview image 2 Estimating the PLT concentration level Note When a slide is opened for the fir
63. es failed or the order was not found in the LIS slide processed with default values o Processing error No result exists Slide status x Empty field Not signed ae Signed Comments dj Empty field No comments H Comments exist User s Manual 61 GEITH O CellaVision M DM1200 6 1 3 Opening an Order Slide Opening an order automatically opens a slide belonging to it In the same manner opening a slide automatically opens the order it belongs to The currently opened order and slide are always shown in the toolbar Order status Slide status Order AE Slide MT Order 80 1 Double click on an order in the Order list and the first slide of that order opens You can also click Open in the Order panel In the Slide list you can open specific slides in the same way You may close the order and slide using the Close Order Slide button in the toolbar Close Order and Slide 6 1 4 Protecting an Order You may protect orders to prevent slides from being deleted or archived 1 Select the order 2 Click Protect Unprotect You may select multiple orders using Ctrl or Shift 62 User s Manual CELLAVISION CellaVision DM1200 6 1 5 Searching for an Order Slide You select the orders to be displayed using the Search criteria Here you may specify date intervals for desired orders Use View latest as a shortcut for orders less than one week old You may also add search strings for patient data order data
64. for how to determine the PLT estimate factor Important You have to determine your own PLT estimate factor By default it is set to 0 Cont d 92 User s Manual e CELLAVISION 6 Cont d Defaults for PLT tab Sets the default values for Grid size PLT count and PLT concentration Intervals for average PLTs HPF value These intervals are used to calculate the PLT result Calculated level 9 7 Adjusting Report Sign Settings In the Report Sign tab you set the template to use in printed reports and the default settings for the signing dialogs MINIMALGerman Default report template created by the system MINIMALItalian Default report template created by the system gt Normal Default report template created by the system NormalFrench Default report template created by the system NormalGerman Default report template created by the system Normalltalian Default report template created by the system Td lt lt Report All available templates are displayed in the list The currently used template is indicated with an arrow To change which template to use select a template in the list and click Set Active Default settings for Sign dialogs Here you set the default values for the Sign Slide and Sign Order dialogs User s Manual 93 J Te 9 8 Adjusting Standard Comments Settings Hypersegmentation Pelger form Hypergranulation Hypogranulation Auer rods Doehlie bodies Pyknotic
65. gazine 2 ug RE er hr EE GG BED GN URDD 21 2 5 2 Starting the Slide Processing rl RR RR RR RR E GR RACER 21 25 32 Magazine SIMMS sew DYD DDO wd UON E Vode YU Y es 22 2 54 Slide US RF TRA AF FYR YR YRS Y RYAN NERFAU YA FR 23 2 6 Electing MaUBZIPB scs on ice o Sandee RR Y C te A HN YNE 24 27 Shutting Down th System uec Ce er RR ERR EEN nr EE y ERE 24 3 QUALITY CONTROL 25 3 1 Cell Location Periornmance lt 20 4 243 bees WE Ex ERES DD ES DDN ED DDAR GOD du 25 3 1 1 Cell Location for Peripheral Blood nannaa aanne 25 3 2 Self TES S ee Ge GA RERO E GRO ORDER DR TEES eaa ea UHR RE e ded a 29 4 VERIFYING PROCESSED SLIDES PERIPHERAL BLOOD 31 4 1 White Blood Cell Classification VVY FY ee 31 4 1 1 Customizing Views cedi bekeRRRERIQNEPIGHRYE Db REdeda were EE IMS 34 4 1 2 Reclassifying White Blood Cells liliis 36 4 3 Email ix REA ERR E ROO CR OE UR acie ge qd KR E S en 38 4 1 4 Copying Images to DISK a keys E EX GYD U REIR Y Yn byd Ri YF ph 39 4 2 Red Blood Cell Characterization llle 39 4 2 1 Customizing the Red Blood Cell Overview Image lessen 41 4 2 2 Characterizing Red Blood Cell Morphology lt YFY Y Y a 42 User s Manual 1 CELLAVISION 6 CellaVision DM1200 4 2 3 Excluding the Red Blood Cell Analysis VVY YY ug 42 4 3 Estimating Platelets 1 2 eret RD ER pe Sk a 43 4 3 1 Counting Platelets in the Overview Image V
66. gazine PID 6699666666 eg 12 51218539 11 01818831 10 31818533 AGB 3 71018531 Analysis failure Slide oof 8 01918331 eof 7 01310535 oof 5 11118581 QQ 5 94118921 4 Empty oof 3 83118441 2 01144531 Default values 1 01118531 Ho 2277777777 H 8888887788 Magazine To empty the log from all information click Clear Log 2 5 3 Magazine Status The following information is available for each magazine Magazine status Magazine ID barcode PB2345678912 Magazine Status Analyzing X Finished The slide holder is processed and all slides have status OK A wanmiug A slide with status Warning Stopped Failed or Cancelled exists in the magazine amp Failed The magazine has no magazine ID 22 User s Manual CELLAVISION CellaVision DM1200 2 5 4 Slide Status The following information is available for each slide Slide status Slide position irer aces Prot WATNE barcode text A 7 PB2345678912 Default values Slide Status Processing d OK Slide processed with no warning or error Slide processed with a warning see 4 Watang warning texts below Results exist Slide processing stopped by user No 2 stopped results exist o Eiro All ordered analyses failed see error texts below No results exist Eb Cancelled The slide was cancelled in the LIS Slide not processed Empty Empty Empty slide position in the magazine Warning Texts Default values The or
67. ght part of the screen is an enlargement of the area inside the red rectangle shown in the Mini map Click on the Mini map to enlarge another part of the sample A blue trace in the Mini map indicates which parts of the overview image that have been viewed by the operator Hold left mouse button down and scroll in any direction using the mouse Switch between 10x and 50x zoom levels by right clicking in the enlarged image Adjust the zoom level by dragging the sliders in the Zoom panel Zoom 10x e 50x 73 CELLAVISION 6 CellaVision M DM1200 7 2 3 Tagging Regions of Interest To save an enlarged area as a region of interest for later reference click Tag region Thrombocyte aggregate Blasts Tumor cells 74 User s Manual CELLAVISION CellaVision DM1200 7 2 4 Copying Regions of Interest to Disk You can copy a region of interest to disk 1 Select a tagged region of interest in the list 2 Right click and select Copy the image to disk 3 Specify the destination path where you want the image to be saved 4 Click OK 7 2 5 Adding Comments To add a comment to the Overview click Scan comment Scan comment 7 2 6 Order Data To view information about the order e g scanned area and comments click Order Data in the toolbar The Order Data dialog can also be accessed by right clicking on an order in the database
68. ibes may be reproduced or transmitted by any means or in any form without prior consent in writing from CellaVision AB U S patents no 7450762 6341180 6268611 and 7034883 Swedish patent no 9902863 German patents no 602004008471 French patents no 1646964 U K patents no 1646964 Caution US federal law restricts this device to sale by or on the order of a physician or properly licensed medical practitioner Article No PM 10364 Revision 2009 06 17 Copyright 2009 CellaVision AB All rights reserved Headquarters CellaVision AB Ideon Science Park SE 223 70 Lund Sweden Phone 46 0 46 286 44 00 Fax 46 0 46 286 44 70 6 User s Manual CELLAVISION CellaVision DM1200 1 introduction 1 1 About this User s Manual This User s manual will guide you step by step through the activity sequence of normal use of CellaVision DM1200 also referred to as the system aiming for an efficient way to give you good understanding and knowledge of the system and its features References are made to appendices providing additional information Some self explaining dialogs have been left out in the text Typographical conventions e Names of keys and on screen objects with which you interact are presented in italics e g click PRINT 1 1 1 Warnings and Precautions Study the meaning of symbols and safety alerts carefully and always use the system in the safest possible manner Read all instructions carefu
69. ion Always use protective gloves and tweezers when removing glass shards from the system Open the animated tutorial goto Help Tutorial Click Gripper service Move Gripper to Service position a Goto Maintenance Gripper Service b Click OK c Wait until the gripper is in service position dialog appears d Open the hood and remove the slide e Close the hood f Restart the SSU and the progam User s Manual 119 CELLAVISION 6 CellaVision M DM1200 13 CellaVision Software 13 1 CellaVision Remote Review Software CellaVision Remote Review Software gives remote users access to analyzed slides and the possibility to reclassify cells and sign slides from another location Smears needing a second opinion can easily be reviewed by a pathologist from within or outside the hospital Networking and collaboration within a hospital or between hospitals results in staffing flexibilities CellaVision Remote Review Software is an additional software product to CellaVision DM Analyzers CellaVision Remote Review CellaVision DM Analyzers The operating procedures in Database View Verification View and Report View are the same for CellaVision Remote Review as CellaVision DM Note It is not possible to process slides or archive data using CellaVision Remote Review Software User s Manual 121 e MC 13 1 1 Working with Orders Slides When opening an order slide with CellaVision DM1200 or a CellaVisi
70. ion of the system If the LIS communication is enabled the program will also check the connection to the LIS After each slide the system has processed it checks the positioning of the motors While the program is running the database size is compared intermittently to the rules set for archiving or automatic deletion of old entries thus ensuring that the database is kept at a reasonable and maintainable size The communication with and response of the hardware is tested continuously during the operation of the system and a message will inform the operator if an error occurs during slide processing or other operations on the system User s Manual 29 NN CellaVision M DM1200 30 User s Manual CELLAVISION 6 CellaVision DM1200 4 Verifying Processed Slides Peripheral Blood Opening an unsigned slide leads directly to the Verification View where various tabs can be selected in order to review WBC RBC and PLT and to Sign the Slide To open a slide see 6 1 3 Opening an Order Slide Click Verification View in the toolbar Note With DMConfiguration tool it is possible to add user defined WBC cell classes and RBC characteristics To do this contact your service personnel 4 1 White Blood Cell Classification You can view all WBCs identified by the system You may also reclassify WBCs and add comments WBC panel Opened Main Add cell Shortcutsto 2nd gallery order slide gallery class reference comment cells
71. itting Cells If more than one cell is displayed in an image it can be difficult to determine which cell the system has actually identified Click Cell Marker and a green square appears marking the cell t4 Cell Marker Sometimes the system fails to separate WBCs and the green square will cover more than one cell It is now possible to split cells using the right click menu and select Split cell A dialog appears explaining the procedure The green square is never changed for split cells Instead if you click on Cell Marker a red cross marks the cell Note Cells created by splitting can always be removed using the right click menu WBC Attributes Each cell is associated with attributes Indicates the order Cell has been 1 vy Reclassified cell in the class e 9 forwarded from k another cell class I _ Cell comments exist o Cell is selected for D B m e mail WBC attributes are shown by default Click WBC Attributes to show hide them 9 WBC Attributes Cont d User s Manual 37 NC CellaVision M DM1200 Cont d Right click Menu Right click on a cell to set view attributes A menu appears allowing the following options View DM s 1st 2nd and 3rd suggestions of classification Reclassify cell Add view cell comments see 4 4 Comments Split cell or remove split cell Select cell for e mail NOB WN Save cell as custom reference cell 7 Save images to disk
72. l dip method 148 User s Manual CELLAVISION CellaVision DM1200 Cont d Wright Giemsa Stain Note Differences in staining results may be cause by alterations in pH reagents etc Local adjustments may be needed to achieve best results Be aware of pH variations in water Beckman Coulter LH755 slide stainer or manual dip method Solution Reaction time Methanol 1 min 30 sec 2 Wright Giemsa stain 3 min 30 sec 3 Wright Giemsa stain diluted 1 10 with phosphate 3 min buffer pH 6 8 Rinse in pH adjusted distilled water 1 min 1 min 5 Rinse in pH adjusted distilled water 1 min 1 min User s Manual 149 NN CellaVision M DM1200 150 User s Manual User s Manual CELLAVISION CellaVision DM1200 Appendix H Glossary Analysis Confirmation Duplicate slide differential Grid square In vitro LIS Magazine Magazine ID MGG Mini map Multi slide order Non WBCs Not classed Operator Order Order ID Patient ID Pending order PID PLT PLT estimate PLT estimate factor Precharacterization Preclassification Processing slides Quick scans RBC Reclassification Reference cells Description Starts with the loading of slides and ends with the finished report The operator approves the preclassification Two different persons sign one slide each in the order The average result is presented Sub images of a PLT overview Outside
73. licking and dragging in the slide image The Min Max following constraints apply x mm 16 67 mm zai Um i El 6 5 lt Xmin lt Xmax lt 19 5 mm 6 5 lt Ymin lt Ymax lt 50 0 mm Yes imm 2883 mm The width and height of the scan area must also satisfy 2 0 lt width lt 20 0 mm HT 2 0 lt height lt 20 0 mm Note If no scan area is defined the default scan area setting will be used User s Manual 77 CELLAVISION 6 CellaVision M DM1200 7 4 Customizing the System To customize the system go to the Tools menu and select Settings 7 4 4 Adjusting Database Users and Language To adjust the settings for database users and language see 9 1 Adjusting Database Settings 9 2 Adjusting Users Settings and 9 12 Adjusting Language Settings 7 4 2 Adjusting Default Scan Area To adjust the default scan area go to Tools Settings Default Scan Area Default Scan Area The scan area can be selected either by using the edit boxes or by clicking and dragging in the slide image The following constraints apply Min Max x fio mm 15 67 mm v 17 83 mm 28 84 mm 6 5 lt Xmin lt Xmax lt 19 5 mm 6 5 lt Ymin lt Ymax lt 50 0 mm The width and height of the scan area must also satisfy 2 0 lt width lt 20 0 mm HUI 2 0 lt height lt 20 0 mm To adjust the scan area settings for individual slides click Da
74. lly before starting to use the system Using it without being suitably qualified or in a manner not specified in this User s manual may damage or deteriorate the system cause misleading results or even lead to injury Warning alerts appear in this manual as follows Alert Explanation WARNING May cause injury Caution May cause damage to the system Important May cause misleading results Cont d User s Manual 7 CELLAVISION 6 CellaVision M DM1200 Cont d Place the system on a steady table Do not place it where it is exposed to bumps or vibrations excessive temperature variations or direct sunlight The system must be connected to grounded electrical sockets only Authorized personnel should do the initial installation and reinstallation after moving the system Do not install or run any software not supplied with the system To maintain electromagnetic compatibility use only original components Spillage of fluid on the surfaces of the system may cause malfunctions or deterioration Wipe off spilled fluids immediately with a soft tissue WARNING The system should be serviced by authorized personnel only WARNING If the eguipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired The following symbols are found on the system Symbol Explanation A Documentation needs to be consul
75. m computer at least once a week Controlling the Database Size A large database will have lower performance access time database search time etc compared to a smaller database The size of the database can be controlled by two methods 1 Activate autodelete of orders 2 Archive images onto CD R RW or LAN Recommendation Keep the maximum size of the database to approx 20 GB See 9 11 Adjusting Archiving Settings for more information Note Select Compress Database to view the database size see 9 1 5 Compressing a Database Note Delete all unsigned and failed orders User s Manual 107 NN CellaVision M DM1200 108 User s Manual CELLAVISION CellaVision DM1200 12 Troubleshooting 12 1 12 2 1 User s Manual Troubleshooting Steps If a problem occurs follow these steps 1 Observe any unusual circumstances surrounding the problem 2 Note any error messages displayed 3 See 12 2 Troubleshooting Chart to find possible remedies 4 If the problem remains contact your distributor for assistance 12 2 Troubleshooting Chart General Startup Problems Could not log on to the system Make sure you typed your username and username and or password Problem Cause Action Message box Previous Make sure the program is closed Wait The program has already program has 10 seconds and try again been started and you not closed cannot start another until th
76. maintenance This operation can not be performed at this time Communication error between SSU and system computer Restart the SSU and the program Communication failure No connection between the Check the connection between the SSU and the system computer Check SSU and the power connection to the SSU Restart system the system and the program computer Temperature is too high The air filter is Check that the air filter on the back of dirty the SSU is not dirty Change if needed The fan inthe The fan has to be exchanged control unit does not function Oil dispensing failed The oil bag is Change oil bag see 10 3 1 Change empty Immersion Oil Pack File system or registry The File system Reinstall the program failure or registry is corrupt Internal error Temporary Restart the SSU and the system software error computer Magazine eject failure The magazine is stuck Remove the magazine manually Restart the SSU and the program Positioning failure At least the motor specified in the message box reported a Move gripper to service position and remove slide see 12 2 8 Gripper Service Restart the SSU and the program If error persists contact your positioning local vendor s technical support failure The slide is Move gripper to service position and stuck or broken remove slide see 12 2 8 Gripper Service Restart the SSU and the prog
77. mple Preparation To perform a peripheral blood differential count a thin blood film is wedged on a glass slide a blood smear from a peripheral blood sample and stained with Romanowsky stain see Appendix G Slide Preparation Guidelines for recommended staining recipes User s Manual 11 TM CellaVision M DM1200 1 4 Components and Mechanical Operation 1 4 4 Major Parts of the System The system comprises the following major units Computer system Slide scanning unit SSU Motorized microscope Digital color camera Immersion oil unit Robot gripper unit Barcode reader Control unit Casing Computer System A PC system running Microsoft Windows XP and CellaVision DM software 12 User s Manual CELLAVISION CellaVision DM1200 Slide Scanning Unit Motorized Digital color Immersion Control Hood microscope camera inside oil unit unit Gripper Sd Magazine Status lamp Power on Main switch pace holder lamp WARNING Never tamper with sensors or other safety devices These make sure that the system can operate without any risk of personal injury Users Manual 13 CELLAVISION 6 CellaVision M DM1200 Motorized Microscope The motorized microscope is an upright light microscope with a LED illumination system It has one 10x objective and one 100x objective and intermediate optics switching between 1
78. nts For each slide you can add comments to the WBC RBC and PLT results For WBC analyses you can also add comments to cell classes and individual cells WBC RBC and PLT comments are added in Verification View in each tab respectively Cell class and cell comments are added in the WBC tab in Verification View Note All comments except comments on individual cells are printed in the report 46 Users Manual CELLAVISION 6 CellaVision DM1200 4 4 1 Adding Comments The system records the author of each comment except for comments to cell classes and individual cells If another operator logs in each comment is tagged with the name of the operator who wrote it An operator can view all comments but can only edit his her own Comment added by operator admin omments Comment added by operator author Click Comments to add comments to WBC RBC and PLT WBC comment Hypersegmentation Pelger form Hypergranulation Hypogranulation Auer rods Append You may write edit comments in the Comment box Click Standard Comments to show hide standard comments Double click on a standard comment to add it to the Comment box You may also select a standard comment and click Append Cont d User s Manual 47 CELLAVISION 6 CellaVision M DM1200 Cont d You may activate the Comment types you want to display Standard comments of type WBC RBC or PLT will only be available in the respe
79. on Remote Review the order and the slide will be locked for other operators In all other CellaVision Remote Review and CellaVision DM systems connected to the database this is indicated with red text in the Order list and Slide list in the Database View see 6 1 Database Processed Orders A locked order slide can be opened but not modified i e no changes can be done in Verification View and Report View In the Order data and Slide data panels Locked by indicates who has opened the order slide the person logged on to Windows and on which computer 13 2 CellaVision Competency Software CellaVision Competency Software is a program for education and competency testing of manual blood cell differentials in laboratories The product allows labs to ensure that staffs are trained to report results consistent with lab policies E En _ Agreement for Myelocyte All Participants compared to Examiner WOC Once ater ML neo EX ten ETE WY ED CD ERD recon ET rcm as Coll cimc cdicabions a All colts Cell Chane Clas idis otona rie A peyton yio More vad loom Lage gamer hme Count nbr of cells rl s Ddw HM Cirt CellaVision Competency Software prints graphs and charts allowing identification and documentation of areas for improvement 122 User s Manual CELLAVISION CellaVision DM1200 Appendix A System Specification Climate Specification CellaVision DM1200 i
80. on and staining time may be increased Overwashing The washing technique may be eosinophilic corrected so that it is adequate but not granules excessive The erythrocytes appear blue or green the nuclear chromatin is deep blue or black and the granules of the Overstaining Decrease the fixation time The amount of stain used may be decreased shorten the staining time and the amount of buffer increased increase the stain buffer time neutrophilic Inadequate Increase the wash step granulocytes are washing deeply overstained Too high an The pH of the buffer may be checked and appear large and prominent The granules of the eosinophils are blue or gray alkalinity of stain or buffer with a pH meter and readjusted to a lower pH Thick blood smears Prepare a thinner blood smear Samples have dark areas of stain or other artefacts Drying is occurring during the period of staining Ensure the adequate drying of samples previous to staining Unclean slides Use clean slides Inadequate washing not washing enough to remove the metallic scum Increase wash step A stain is forming precipitate in the solution Filter the stain Ensure that the stain has not exceeded the expiration date User s Manual e CELLAVISION 12 2 8 Gripper Service WARNING A broken slide can cause serious cuts and poses a danger of infect
81. ored in the system Email settings Language Select Tools followed by Settings and the appropriate tab to customize the system Different users are allowed to edit different settings see Appendix E User Authorization Levels User s Manual 83 NN CellaVision M DM1200 9 1 Adjusting Database Settings Here you create new databases and new database connections In the Log On dialog you select which database to use It is also possible to compress an existing local database 20030032 dm96im Processing Database from installer ScanDB Scan Disconnect IZOImpress G Local database a Remote database 84 User s Manual CELLAVISION CellaVision DM1200 9 1 1 Creating a New Database 1 Click Create A dialog appears where you may enter Database name Description Home directory and Database size 2 Select one of the following types of database e Processing database a database for processing slides e Export database a database that is used when you want to export orders from another database This type cannot be used for processing slides e CellaVision Competency Software database a database for use with CellaVision Competency Software A CellaVision Competency Software database cannot be used for processing slides e Scan database a database for scanned slides 3 Click Create A new folder with the chosen Database name will be created in the specified Home
82. ounter on 30 consecutive blood samples 2 Prepare and stain one smear for each sample 3 Perform PLT analyses on the system Let the system calculate the average PLTs HPF values for each sample 4 Divide the cell counter PLT value by the system average PLTs HPF value for each sample to get the conversion factors 5 Add all 30 conversion factors and divide by 30 to get the PLT estimate factor 6 Enter the PLT estimate factor in the PLT tab in Settings User s Manual 141 NN CellaVision M DM1200 142 User s Manual CELLAVISION CellaVision DM1200 Appendix G Slide Preparation Guidelines Slide Preparation for Peripheral Blood WARNING Always use protective gloves when in contact with blood Sample Collect blood from a vein or by skin puncture in an EDTA sample tube K2EDTA or K3EDTA 1 5z 0 15 mg ml in liquid or powder form Mix the sample carefully with the anticoagulant Store the tube at room temperature Prepare the blood films within four hours of blood collection Preparing Blood Films 1 Mix the tube 20 complete inversions by hand before preparation 2 Use a clean dry microscope glass slide see Appendix A System Specification for required slide types Note that glass slides may lose their wetability on exposure to air resulting in a poor smear 3 Use the wedge technique performed manually or by a mechanical spreader Manual wedge technique Place a drop of blood near the l
83. pped or round corners Cont d User s Manual 125 Y CellaVision M DM1200 Cont d Slide descriptions Clipped corners Round corner Images provided by Erie Scientific Www eriesci com Caution Use only clipped round corner slides Failing to do so may cause jams and excessive wear on magazines and the system Caution Using the magazines more than 100 times may damage the system Barcode The system reguires high guality barcode labels on the slides Maximize the barcode width for best result Note The magazines are supplied with barcode labels If you replace the barcode label the new barcode must not contain the 7 character Barcode Scanner The following barcode formats are accepted by the system UPC A Interleave 2 of 5 CODE 11 UPC E Industrial 25 China Postage EAN 13 Matrix 25 MSI PLESSEY CODE 39 CODABAR NW7 CODE 32 CODE 128 CODE 93 BC412 The minimum barcode resolution shall be 7 mil Cont d 126 User s Manual CELLAVISION 6 CellaVision DM1200 Cont d The label should be positioned as shown in the figure below The edges of the label shall be at least 1 mm from the edges of the slide Note Avoid oil on the slide label Barcode length UBL t Barcode width io rn m or 2D D Barcode Scanner The following barcode formats are accepted by the system Linear codes CODE 39 CODABAR NW7 Code 128 Interleave 2 of 5 2D D codes DataM
84. ppendix C Buttons and Indicators System status is shown as a text Idle Analyzing Stopped Paused or Error System status System indicators Ansbicis progress WBC Count x10e9 L Unidentified 11 75 0 0 3 Cells counted 146 of total 400 Band neutrophil 2 Esti tene ert 4 51 mi Segmented neutrophil 39 257 0 0 mE 3 Eosinophil Basophil 3E LS jo o Lymphocyte 72 493 00ENEN 731 Peripheral blood analysis started Monocyte 18 123 008 1 Searching for monolayer y Monolayer search completed Promyelocyte z 5 e x 3 a 5 en e DA OD Myelocyte Searching for RBC PLT monolayer Metamyelocyte precede Blast no lineage spec 1 07 0 0 L 3 Pre classifying WBCs Lymphocyte variant form Zi s mimi using high power magnific IRBC pre characterization complete if ordered Plasma cell Other Total 146 100 0 0 Non WBC Count Erythroblast NRBC Giant thrombocyte 21 144 Thrombocyte aggregation Smudge cell 20 137 Sesto Artefact mi z6 Patient data ae RBC TE TE OA Polychromasia 09000 oo RR Hypochromasia 09 O0OOO on Comment Latest found cells essee 00000 37 E 1 2m eS Microcytosis 04 OOO 30 ios Mid Cue HU a Mecrocytosis 09000 os Medii Y Poikilocytasis o OOO os Slide position 1 d e Slide ID 027 Ie a c Order ID 027 m M
85. r in the middle of the test not only towards the end Cont d 26 User s Manual e CELLAVISION Cont d Su Looking at image X of a total of Y images Overview images Step one image back or forth SKK Image 4 34 Number of nucleated cells in the image WBEs NRBCs found 11 shown WBCs NRBCs missed fi ES Number of missed nucleated cells in the image shown number entered by the user Image status Empty Image has not been viewed a Image has been viewed and has no missed nucleated cells Ti Image has been viewed but contains missed nucleated cells The Result When a slide has been examined a result is automatically calculated The percentage indicates how many nucleated cells the system found including the manually added number of missed cells It is possible to adjust the total number of found nucleated cells if it is determined that some found cells are neither WBC nor NRBC For instance if the system identifies 187 nucleated cells but the user thinks 3 of them do not fall under this category he she can enter 184 in the Manual correction field and this number will be used in the calculation The cell location results can be printed by clicking the Print Result button Cont d User s Manual 27 CELLAVISION 6 CellaVision M DM1200 Cont d F Total number of nucleated cells and non Total result MW nucleated cells found in all the images WBCs NRBCs found 21
86. ram Magazine is Remove any magazines and slides broken and Load the slides to a new magazine fragments are Restart the SSU and the program If blocking the error persists contact your local slide vendor s technical support The barcode Wipe off oil from the barcode label could not be read after the slide was processed Verify that the hood is closed The hood was open Close the hood User s Manual 111 CELLAVISION 6 CellaVision M DM1200 112 Error Message The slide gripper has unexpectedly lost the slide Cause Gipper has dropped slide Action Pull out bottom tray and remove slide Restart the SSU and the program The slide could not be returned to the magazine Magazine is broken and fragments are blocking the slide Move gripper to service position and remove slide see 12 2 8 Gripper Service Restart the SSU and the program Barcode reader failure The barcode reader cable is disconnected Make sure that the barcode reader cable is connected to both the barcode reader and the system computer Restart the program A slide is not fully inserted in the Magazine Slides not fully inserted Eject the magazine and insert the slide fully Restart the SSU and the program Error Message disconnected Cause Action Errors during analysis No camera found The camera Make sure that the camera cable is cabl
87. rom the bottom up Make sure that slides are fully inserted in the magazine before using the magazine Caution Use only clipped round beveled corner slides Failing to do so may cause jams and excessive wear on magazines and the system Note The same magazine can be used up to 100 times When this limit is reached a warning is shown Caution If you want to process an already processed slide carefully wipe the oil off and make sure the barcode is clean and undamaged 20 Users Manual CELLAVISION 6 CellaVision M DM1200 2 5 Processing Slides View the processing in the animated tutorial Open the animated tutorial goto Help amp Tutorial B Click Analyze 2 5 1 Adding Magazine Slide the magazine into the magazine holder The magazine must have the barcode turned upwards and the open end of the magazine inwards When all slides in a magazine have been processed the magazine is automatically ejected 2 5 2 Starting the Slide Processing The processing starts automatically when a magazine is inserted into the system You have to manually start the processing if the system has been stopped restarted or if an error has occurred Start button 4Y Autostart Replaces the Start button m Stop button Cont d User s Manual 21 MS CellaVision M DM1200 Cont d The tree in the System Control panel displays a status log of processed magazines and slides 4433444444 No ma
88. s a level Changeable results are written in bold text 1 Click on the result to change 2 Change the value in the dialog 3 Click OK Note If you include or exclude a slide all manually changed values will be replaced by new automatically calculated values Cont d User s Manual 53 EST CellaVision M DM1200 Cont d Comment Panel View all comments associated with the order in the Comment panel All comments will be included in the report Note that only the beginning of the comment is shown To view the whole comment click on it It is not possible to edit comments in this panel MT Order 120 2 Comment Comment to this cell class mment to this cell cla Comment to this cell class Comment to this cell cla Comment to this cell class Comment to this cell class Comment to this cell class Comment to this cell class Comment to this cell class Comment to this cell class Comment to this RBC admin Comment to this PLT admin 34 User s Manual CELLAVISION 6 CellaVision DM1200 5 2 Report Preview Click on the tab Report Preview to preview the report The report s format is template based and can be selected in Settings Refer to 9 7 Adjusting Report Sign Settings for more details 5 3 Signing an Order result 1 Click Sign Order Database YourDatabase Type Processing User name Password IV Sign order when signing slide IV Send to LIS
89. s designed to be safely operated at 18 C to 31 C 64 F to 88 F at a maximum relative humidity of 90 with no condensation allowed indoor use and altitudes up to 2000 m Physical Specification Weight 80 kg excluding PC and monitor Size WxDxH 57x49x71 cm 22 4x19 3x27 9 inches Electric Specification monitor not included Voltage input 100 240 VAC Current input for the system computer 6 3A Current input for the slide scanning unit 2 1A Total current input for the system 8 4 A Voltage frequency 50 60 Hz Pollution degree 2 Installation category II Over voltage category II Mains supply voltage fluctuations not to exceed 10 percent of the nominal voltage WARNING Connect to ground sockets only WARNING The mains supply cord and plug of the equipment shall comply with any national regulations WARNING External computing devices connected to the communication connector LAN of the system have to comply with the standard UL 60950 WARNING The mains supply cord shall be used for disconnection from mains supply Cont d User s Manual 123 CELLAVISION 6 CellaVision M DM1200 Cont d Performance Specification Peripheral blood Average WBC cell location and display of at least 97 with a standard deviation less than 2 90 Throughput Approximately 20slides h for complete orders containing RBC PLT and 100 cell WBC Results of short term imprecision found in a clini
90. s help lines for PLT Help Li em estimate Confirm Cell Counter Results Comments WBC Full Screen View WBC Galleries Zoom Mode T CELELEE j B EJ Zoom In Zoom Out Scroll Mode User s Manual 133 Y CellaVision M DM1200 Adjusts image color and Color Brightness brightness Toggles between default color Toggle Color Brightness and lights settings and personal settings Shows Hides sguare for cell GAFR identification i f WBC Attributes Shows Hides WBC attributes pd Entire RBC Image Shows the entire RBC image Start Stop Indicators Q Ai Automatic start of slide processing a Oil Level Indicators Indicates an empty oil pack Indicates need for changing oil a pack hn Indicates a full oil pack Hood Indicator Indicates hood is closed m Indicates hoood is open Keyboard shortcuts Ctrl w Show hide Worklist 134 User s Manual CELLAVISION CellaVision DM1200 Appendix D Recommended Workflow Laboratories handle their peripheral blood differential counts in different ways making it hard to suggest one general workflow suitable for all laboratories See flowcharts of the three workflows below Recommended Settings see 9 7 Adjusting Report Sign Settings 1 Single slide differentials Pre fill password Enabled Sign order when signing the slide Enabled Send order to LIS when signed Enabled Review the cells in the WBC Galleries 2 Confirmation of cell co
91. st time the slide gets mode according to the settings and the mode can then not be changed for that order The system ensures that all slides in a multi slide order have the same mode User s Manual 43 TUNI CellaVision M DM1200 4 3 1 Counting Platelets in the Overview Image PLT Count The estimation of the PLT concentration is based on the number of PLTs which must be counted manually You can choose to count the number of PLTs in each grid square or to specify an approximate number of PLTs per grid square PLT count Grid size 4 x4 0 50 HPF square J ROB PB fF PP PF bL b pP fP F F a tJ Approximate PLTs per grid square Calculate PLT Result a Counting PLTs per grid square 1 Select the Count PLTs per grid square radio button 2 Select the entry fields one by one count the PLTs in the image window and type the number in the entry field You can use tab and Shift Tab to move between entry fields b Specifying an approximate PLT count per grid sguare 1 Select the Approximate PLTs per grid square radio button 2 Use the entry fields to view different grid sguares 3 Estimate the average PLT count per grid sguare and type this value in the field Cont d 44 User s Manual CELLAVISION CellaVision DM1200 Cont d PLT Result 1 Click Calculate PLT Result in the PLT Count panel PLT result PLT estimate factor rs Average PLTs HPF value 23 PLT
92. stained with May Gr nwald Giemsa MGG stain Wright stain and Wright Giemsa stain Dip the slides in the solutions according to one of the following recipes MGG Stain Note Differences in staining results may be cause by alterations in pH reagents etc Local adjustments may be needed to achieve best results Be aware of pH variations in water Alternative 1 Solution Reaction time Change solution 1 May Gr nwald stock solution a 5 min Twice a week 2 May Gr nwald working solution b 15 min Every day 3 Buffer working solution d Quick rinsing Every day 4 Giemsa working solution f 30 min Every day 5 Buffer working solution d Extensive rinsing Every day Alternative 2 Solution Reaction time Change solution 1 May Grunwald stock solution a 5 min Twice a week 2 Buffer working solution d Quick rinsing Every day 3 Giemsa working solution f 10 min Every day 4 Buffer working solution d Extensive rinsing Every day Cont d 146 User s Manual CELLAVISION CellaVision DM1200 Cont d a May Gr nwald stain stock solution Merck M1424 Eosin methylenblue solution modified for microscopy contains methanol Store at 15 C to 25 C b May Gr nwald working solution Dilute 1 part of May Gr nwald stock solution with 1 part buffer work solution Stable for 8 hours c Buffer stock solutions 1 and 2 1 9 07g KH2PO4 0 067M ad 1000ml deionized water Store at 4 C to 8 C 2 9 45 g Na2HPO4 0 067M ad 1000ml
93. stem The information presented depends on your system and installed software Some of the following information is present in the System Information dialog e Serial number of your system The type of Artificial Neural Network ANN used The computer name The computer s IP address Free disk space Free physical and virtual memory Total physical and virtual memory Licensed software License expiry date User s Manual 81 NN CellaVision M DM1200 82 User s Manual CELLAVISION CellaVision DM1200 9 Customizing the System Settings are stored in either the system or the database A setting that is stored in the system applies to that system only Other systems connected to the same database as the system may have other values of the setting A setting that is stored in the database applies to all systems connected to the same database If you change the setting it will be changed for all systems connected to the database The following settings are stored in the database e Restricted user search options User settings Analysis default values Enable LIS Add processed slide to worklist WBC reclassification settings RBC precharacterization settings e PLT settings Active report template Report template contents Default settings for sign dialogs Standard comments Custom reference cells e Autodelete Archiving settings The following settings are st
94. t patient data and orders are not copied or deleted Restrictions Only signed orders can be exported Orders imported to an Export database will always have a protected flag preventing them from being autodeleted if autodelete is enabled Only peripheral blood orders can be exported to CellaVision Competency Software databases Orders residing in a Scan database cannot be exported 6 1 8 Copying Images to Disk You can copy all images from selected orders to disk 1 Click on Database View 2 Select one or several orders 3 Right click and select Copy images to disk 4 In the Copy images to disk dialog specify the destination path where you want to save the images 5 Per default WBC images and Overview images are checked Uncheck according to preferences 6 Click OK Note The specified directory in the destination path must exist 64 Users Manual CELLAVISION CellaVision DM1200 6 1 9 Printing Orders Print order data and data for all slides belonging to it by selecting the order or slide of choice in the Database View and then Print in the File menu Note Order results exist only if the order contains at least one signed slide 6 1 10 The Worklist The Worklist contains shortcuts to slides facilitating an automatic workflow see Appendix D Recommended Workflow The Worklist is active in the Database View and the Verification View to simplify opening of new slides Open slides by
95. tabase View and select Pending Orders See 7 3 2 Pending Orders 78 Users Manual e CELLAVISION 6 7 4 3 Adjusting Analysis To adjust the default analysis settings 10x or 10x and 50x magnification go to Tools Settings Analysis PB default values BF default values Number of WELs ta count Number of WB Us to count Type of order Type of order Iv Diff V Overview f Overview 10 Overview 104 50 v Add processed slide ta worklist Enable LIS To adjust the analysis settings for individual slides click Database View and choose Pending Orders See 7 3 2 Pending Orders User s Manual 79 NS CellaVision M DM1200 7 4 4 Adjusting Autodelete It is not possible to archive the orders All orders will be autodeleted after a specified number of days Autodelete Strategy The autodelete strategy depends on your throughput and the need for saving images for future use The autodelete settings are database specific enabling each database to have its own autodelete strategy Delete all images All orders and images are permanently deleted when the time has expired Keep Region of interest images The orders are not deleted but all images except the Region of interest and Mini map images are deleted when the time has expired 80 User s Manual CELLAVISION CellaVision DM1200 8 System Information In the toolbar click Help and then System Information for information about your sy
96. ted In vitro diagnostic medical device Temperature limitation indicates separate disposal of waste of electrical and a 40 c C X This symbol is only valid in the European Community and electronic equipment b Standby Supply User s Manual CELLAVISION CellaVision DM1200 1 2 Intended Use of CellaVision DM1200 CellaVision DM1200 is an automated system intended for in vitro diagnostic use CellaVision DM1200 e Scans a user defined part of a microscope slide e Automatically locates and presents images of cells on smears from various specimens Is intended to be used by skilled operators 1 2 1 Peripheral Blood Application The peripheral blood application PB is intended for differential count of white blood cells characterization of red blood cell morphology and platelet estimation The system automatically locates and presents images of blood cells on peripheral blood smears The operator identifies and verifies the suggested classification of each cell according to type User s Manual 9 CELLAVISION o CellaVision M DM1200 1 3 General Description of CellaVision DM1200 CellaVision DM1200 consists of an optic unit consisting of a microscope and camera referred to as a slide scanning unit and a computer system containing the acguisition and classification software CellaVision DM software It is important that slide preparation is performed according to standardiz
97. the living body in an artificial environment Laboratory Information System A container transporting up to 12 slides in the system The barcode no on the magazine label for magazine identification May Gr nwald Giemsa a Romanowsky stain for blood smears Overview image of analysis area An order including more than one slide from one sample Cells and objects identified as not being WBCs Cells and objects the operator cannot identify and wants to exclude from the differential count The person who works with the system The name of the ordered analyses on the slides from the same sample Order identifier There can be several slides with the same Order ID but different slide numbers Unique number identifying the patient An order manually added to the database waiting to be processed Positive Identifier The barcode on the slide Platelet thrombocyte Estimation of the PLT concentration A predetermined factor to calculate the PLT estimate The system suggests RBC morphology characteristics The system suggests WBC classification The sequence of events from when the slide holder is put on the conveyer to when they are ejected to the output drawer Quickly scanned slides for cell counter verification The results are not sent to the LIS Red blood cell erythrocyte The operator changes the preclassification Cells with typical characteristics available in the 2nd and 3rd galleries 151 CELLAVIS
98. tion for Peripheral Blood The test establishes how many percent of the nucleated cells i e WBC and NRBC that are found on a slide The Cell Location tool may be accessed in the Tools menu Slide Requirements Use a blood sample with a WBC count in the normal WBC range To reduce processing time a WBC count above 7x10 1 is recommended If the system cannot locate at least 100 nucleated cells the result will be discarded The percentage of non nucleated cells i e all other objects that are not identified as being a WBC or an NRBC e g smudge cells must not exceed 30 of the total number of objects The slides must have a barcode label starting with the text QC AII slides with this kind of label are automatically treated as cell location test slides When the slide has been processed it will only be available in the Cell Location tool Pre printed QC labels are available from your local dealer Running a Cell Location Test 1 Select a slide that meets the requirements and put a QC label on it Put the slide in a magazine place it in the DM and process the slide Go to the Cell Location tool when the slide has finished Select the new slide at the top of the slide list Go through all the images belonging to the slide and examine them for missed nucleated cells Enter the number of missed cells if any in the input field for each image nA BW N 6 When all images have been examined the result will appear at the
99. tting eens 96 9 10 Adjusting E mail Settings s 13529 Cos DD Y DOD o GORDD Gd P 97 User s Manual 3 CELLAVISION o CellaVision TM DM1200 9 11 Adjusting Archiving oeting amp cusesednecdeedee GDG UD DR EURO RW DDR 97 9 12 Adjusting Language Settings FY FFF RF RR Y ud 102 10 MAINTENANCE 103 10 1 Weekly Maintenance ui uoa x ERE RYWE D ERIS Y YUA GAU DDU ee 103 10 1 1 Cleaning of Objectives and LED table 0 00002 e eee 103 10 1 2 Delete Unsigned Orders YF IF RR uu 103 10 1 3 Clean Bottom Plate sc cas os E RE RR DAW WR RA HR wees 104 10 2 Preventive Maintenance Gi Du GE GYG DDU DEYD DD RESG 105 10 3 Remedial Maintenant amp c sexa exea ESRRRRERERRERLRESDed eaves RR RE ERR DUR 105 10 3 1 Change Immersion Oil Pack FFF eee 105 10 4 Database Performanc 6 see RR RR WU kx eee RR REA 107 10 4 1 Controlling the Database Size 0 0 cee ee 107 12 TROUBLESHOOTING 109 12 1 Troubleshooting Steps sc aca e p be x8 obs eed eod apodo ale AD OG Gra QR d eo np 109 12 2 Troubleshooting Chart i i sue RR Rr eh REG RR EDR RR RR RR Re REOR Rn 109 12 2 1 General Startup Problems FY YR RR uu 109 12 2 2 ETror Message LiSt 5 GU RP DG FED bide toni OO se KC 110 12 2 3 General Processing Problems REY RR EE RE ded RE EE 113 12 2 4 LIS EITOIS uendere EE ed NE ERN REEL ue ceed eed Wer ERE 115 12 2 5 Cell Location Problems a ue re k
100. unter results Quickly scanned slides for verification of cell counter results Pre fill password Enabled Sign order when signing the slide Enabled Send order to LIS when signed Enabled Review the analysis type you want to confirm and then click Confirm Cell Counter Results Confirm Cell Counter Results When using the Confirm Cell Counter Result for any analysis type WBC RBC or PLT the normal sign slide checks cells in the Unidentified class all cells reviewed etc are disabled Note When using Confirm Cell Counter Results the results sent to the LIS are a WBC confirmation flag a RBC confirmation flag and or a PLT confirmation flag For a slide it is possible to report the WBC result and a RBC confirmation flag and a PLT result or any other combination 3 Duplicate slides An order consists of two slides Two persons sign one slide each Pre fill password Enabled Sign order when signing the slide Disabled Send order to LIS when signed Enabled Review the cells in the WBC Galleries User s Manual 135 CELLAVISION 6 CellaVision M DM1200 Workflow Single Slides Confirm Cell Counter Results Go to Database View Shift F1 Click Add to worklist Double click on the first slide in the Worklist Verify WBC Click RBC tab Perform RBC review Click PLT tab Perform PLT review Click Sign Slide tab Sign the slide The next slide in the Worklist will automatically be opened
101. urry barcode caused by immersion oil on the barcode Action Make sure the barcode is resistant to immersion oil Print the barcode as defined in Appendix A System Specification There is no quiet zone Make sure there is a quiet zone according to specifications and that the barcode is not printed too close to a logotype if applicable Symbology contrast is too low Adjust the printer Use slides with a white smooth frosted area The barcode is damaged barcode cells are missing Adjust the printer Use slides with a white smooth frosted area User s Manual 117 CELLAVISION 6 CellaVision M DM1200 12 2 7 Staining Problems 118 Problem The red cells appear bright red the white cells will appear indistinct with pale blue rather than purple nuclei and brilliant red eosinophilic granules will be Seen microscopically Cause Understaining Action The fixation and staining time may be increased Overwashing The washing technigue may be corrected so that it is adeguate but not excessive Use of stain buffer or wash water that is too acidic The pH of the buffer may be checked with a pH meter and adjusted The increased acidity is due to exposure of the stain or buffer to acid fumes Use a new batch of stain or buffer Pale inadequately stained red cells nuclei or Understaining The fixati
102. uter Check the connection between the SSU and the system computer Check power connection to the SSU Restart the system and the program The hood was open The hood was Close the hood Restart the system in the message box reported a positioning failure during startup open and the program Jam error occurred during At least the Restart the SSU and the program If initialization motor specified error persists contact your local vendor s technical support Internal error Temporary software error Restart the SSU and the system computer Temperature is too high The air filter is Check that the air filter on the back of dirty the SSU is not dirty Change if needed The fan in the The fan has to be exchanged control unit does not function File system or registry The File system Reinstall the program failure or registry is corrupt A slide was detected in A slide is in the Move gripper to service position and the gripper during startup gripper remove slide see 12 2 8 Gripper Service Restart the SSU and the program Barcode reader failure The barcode reader cable is disconnected Make sure that the barcode reader cable is connected to both the barcode reader and the CCU Restart the program User s Manual CellaVision M DM1200 CELLAVISION Error Message Cause Action Hardware errors during analysis eject
103. view To edit order data click Order Data Mm in the toolbar User s Manual 75 TUM CellaVision M DM1200 7 3 Database View To view processed and pending orders click Database View Click Database View in the toolbar 7 3 1 Processed Orders Scanned slides are automatically signed For more details about Processed orders see 6 1 Database Processed Orders 7 3 2 Pending Orders A pending order has manually been added to the database and has not yet been processed Pending Orders are useful when defining scan area for individual slides D Pending Orders Order ID Patientin FirstName Last Name Birth date Nbr of cells MT Order 310 Mike Lawson MT Order 320 Nicky Clark Add Edit Delete To add an order click Add To view or edit order data double click on an order or click Edit Cont d 76 User s Manual CELLAVISION 6 CellaVision M DM1200 Cont d Order Data Order ID wr Order 350 Patient ID Scan Area amp Magnification First name Magnification Area Last name C 10x Birth date 10x 50x Gender Order comment Ethnic origin E J Ward Patient comment Sample date Ordering physician zl required field CIS To define scan area for the slide click Area Define the area that you want to scan on this particular slide Scan Area The scan area can be selected either by using the edit boxes or by c
104. y ERE XE eee RE REIR ROW eee ee 64 6 1 9 Printing OII iu s esuuerR qued ARS CER DDEG DOG O Medes 65 6 1 10 The WOINISL 23555548 e Re ee cro Ee Rd dC caged s E edicta cud fos 65 6 2 Database Pending Orders cu YY ya E eed ieeea dee tad ae E gm E RT 66 6 2 1 Peripheral Blood Pending Orders Dialog llli 67 6 3 Archiming Data se ea RR CR RD yR ER I EI CE Es NER quad 67 6 4 lsaseLog cowes e Re EORR EE Eae do i e ci ose doo deca peo dc 68 5 4 1 Statisties 222652 YRR AFF NEA uela 842 LES IOad hs PERS EP hese tod IS 68 O42 opeciticalloli 2 4 cu EE GW O DU UED GO OU eR DOW bed Pd 68 6 5 Export Log Files ET ei ors CA FC EEF EF au wawa ramus GN ED 69 6 6 Backup and Recovery of the Databas6 Fu aa 69 2 User s Manual CELLAVISION CellaVision DM1200 7 DIGITAL SLIDES 71 ZA SCAnning ASNOG P Tr LTTT 71 7 1 1 Slide and Magazine Reguirements LYFLF FL RR a 71 7 2 Scan Overview TU GG EGLUR DD DD YD oL 72 121 OVEIVIBW ss serae E UE ER COR E exer EK Oh UO GYDD 72 T 22 Navigating WEE TUTTI m 73 7 2 8 Tagging Regions of Interest VY FF RF uu Yu 74 7 2 4 Copying Regions of Interest to Disk VY eee 75 2 25 Adding GOrmimelils es Gw iw GR UO DE DRDD WD aun dae Yn OO dees 75 7 2 6 Order Data EF anaana anaa 75 7 3 Database VIEW WR YN ER WR CE RR ER RR eS AR Ree A ews 76 7 3 1 PrODOSSOU OEI LLID GU GDG GADO DOD 20420
105. yte aggregation Megakaryocyte Smudge cell Artefact Click Add to add custom reference cells from disk Valid file formats are JPEG jpg and bitmap bmp All cell images will be stored in the JPEG format You can also store cells from processed slides see Right click Menu in 3 1 2 Reclassifying White Blood Cells as custom reference cells Click Delete to remove the selected custom reference cell 96 User s Manual CELLAVISION CellaVision DM1200 9 10 Adjusting E mail Settings Enter Default recipient of e mail specify Mail server and E mail from i e sender of e mail E Mail Default recipient doctor hospital com Mail server mailserver hospital com E mail from Joperator hospital com 9 11 Adjusting Archiving Settings For storage safety reasons and limited hard disk capacity it is recommended that you regularly archive the cell images or delete the orders When you archive the cell images and comments are transferred from the database to a CD or a hard disk However if you delete an order with archived images you will not be able to access these images again Cont d User s Manual 97 J ETTE Cont d Archiving Autodelete Strategy Warn when the following two conditions are met 1 The number of signed orders exceeds 300 Autodelete 2 These orders are older than 30 days o madis Path for archiving Network path or local drive i c Documents and Settings CVUser

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CellaVision® DM1200 User`s Manual 3.0