Centrifuge 5702 / Centrifuge 5702 R / Centrifuge 5702 RH
Contents
1. Rotor Adapter Max centrifuging Max rcf g force radius cm Imax rounded A 4 38 1 5 2 0 ml 13 4 2 900 Round bucket 1 1 1 4m 13 2 2 850 2 7ml 13 2 2 850 2 6 7ml 13 2 2 850 4 10ml 13 2 2 850 9 15ml 13 0 2 800 15 ml Falcon 13 7 3 000 25 ml 13 5 2 900 50 ml Falcon 13 5 2 900 85 ml 13 5 2 900 A 4 38 5 7 ml 12 7 2 750 Rectangular bucket 9 ml 127 2 750 15 ml 12 7 2 750 20 ml 12 7 2 750 25 ml 12 7 2 750 A 8 17 15 ml Falcon 12 8 2 770 15 ml DIN tube 12 8 2 770 F 35 30 17 15 ml 12 7 2 750 Outer ring 15 ml Falcon 12 7 2 750 F 35 30 17 15 ml 10 7 2 300 Inner Ring 15 ml Falcon 10 7 2 300 F 45 24 11 0 2 ml 6 6 1 430 0 4 ml 8 2 1 770 0 5 0 6 ml 7 4 1 600 1 5 2 0 ml 8 2 1 770 F 45 18 17 Cryo without adapter 9 1 1 970 with adapter 8 9 1 930 To ascertain the maximum rcf for a specific adapter you can apply the following formula as per DIN 58 970 ref 1 118 10 9 n raa n rotational speed in 1 min fmax max centrifuging radius in cm Example The 85 ml adapter has a maximum radius of 13 5 cm At 4 000 rpm a maximum rcf of 2 415 x g is reached 3 Operation 3 12 Adjusting the acceleration and braking ramps When working with Ficoll density gradients or other sensitive applications e g certain types of blood and urine centrifugations slower acceleration and braking ramps can be switched on for the Centrifuge 5702 5702 R 5702 RH This ma2. Centrifuge 5702 Centrifuge 5702 R Centrifuge 5702 RH eppendorf Centrifuge 5702 5702 R 5702 RH Tastenk rzel Shortcuts Display Task Lid Press pid 5702 R d 5702 RH g Parameter change Display Display 3 8 during centrifugation IB flashes flashes closed gt 2 sec 5 sec 5 sec Brake L br on br on 3 12 ON OFF br OF br OF open gt 5sec Signal L b on 3 14 ON OFF b OF q open gt 2sec Parameter lock L Lo on A A 3 15 ON OFF B Gor Geen LLO OW open gt 2sec Program E 1 set parameter prog 1 3 16 only Centrifuge 5702 R RH 1 or 2 prog 2 B 2 sec At set rpm Y y 3 13 Xxx open gt 2sec No part of this publication may be reproduced without the prior permission of the copyright owner Copyright 2012 Eppendorf AG Hamburg Abbildung 1 Figure 1 Centrifuge 5702 Abb 1 Fig 1 2 3 speed time stand by short See 1 Netzschalter und stecker 1 Mains switch and plug 2 Rotormutter 2 Rotor nut 3 Rotor 3 Rotor E E Notentriegelung Emergency lid release Abbildung 2 Figure 2 Centrifuge 5702 R Abb 2 Fig 2 1 2 3 E C temp time short Netzschalter und stecker Rotormutter Rotor Notentriegelung Kondenswasserschale nur 5702 R 1 2 3 E C prog 1 2 speed stand by open fast temp Mains switch and plug Rotor nut Rotor Emergency lid release Condensation water tray only 5702 R
3. A 4 38 5702 720 003 and 5702 740 004 A 8 17 5702 700 002 The date of manufacture is engraved on them in the format 10 01 October 2001 Transparent aerosol tight caps made of polycarbonate have a maximum service life of 3 years The date of manufacture is embossed on them in the form of a clock RS N Aerosol tight caps may lose their sealing strength when exposed to organic solvents e g phenol chloroform Check the aerosol tight caps regularly for changes or cracking Aerosol tight caps showing cracks or other changes must be replaced immediately Rotors caps or buckets which have been damaged by chemical or mechanical factors or which have passed maximum service life may no longer be used Transfer If the device is passed on to someone else please include the instruction manual Disposal In case the product is to be disposed of the relevant legal regulations are to be observed Information on the disposal of electrical and electronic devices in the European Community The disposal of electrical devices is regulated within the European Community by national regulations based on EU Directive 2002 96 EC on waste electrical and electronic equipment WEEE According to these regulations any devices supplied after 13 08 05 in the business to business sphere to which this product is assigned may no longer be disposed of in municipal or domestic waste They are marked with the following symbol to indicate this As disposal regulat
4. Front Centrifuge 5702 RH baugleich mit Centrifuge 5702 R Front of the Centrifuge 5702 RH is structurally identical to that of the 5702 R Contents 24 Introduction er a a a a es a re 25 Intendediuse 22 52 4 een aan Beurer 25 D livery package i rc ee E a a oed meine hk Soke ehe re depre tig dee don wehren id eet 25 Installing the device 32 0 rinite ee an een ee ee bbe ed 25 Safety precautions and applicational limitations Husnennen nennen nennen nennen 26 Operation ETT 28 Functional and operator control elements 00 cece eee 28 Fitting and removing the rotors 0 rraren arena 28 Loading the TOtO S 22224420 ne Bes bee Ego e pe edet E e einsenden 28 Fixed arngle rotors 2 zu en MARE NAME so ee RE RP dees E Ru RR RT ede ma EORR 29 Swing bucket rotors 2 ehh eram 30 Operation of round bucket with aerosol tight caps lsssseseleee e een nn 31 Centrifugation with timer setting lssieleeeese RR RI hr 31 Changing the centrifugation parameters during the run 0 0 cette tenes 31 Short centrif gatiOnl 9 xar x etes eu Ee re e meats ARE Bad alg anew el nude RR caa Ge RR UR d ane Dele uc 31 Continuous UNNIN sss tios aire aside meer a acta adele Beata tae nno du de A xine mob Aah Rin t 32 Ret display and calculation sire con 22 ar ana a ara da rn 32 Adjusting the acceleration and braking rampS 1 2 0 0 ete ee 33 AUSCUIDM are tet atte sr Eg dete wad eae aaa eee
5. Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part I General Requirements Part 2 Particular Requirements for Laboratory Centrifuges CAN CSA C22 2 No 1010 2 020 92 CAN CSA C22 2 No 1010 2 020A 97 See the UL Online Certifications Directory at www ul com database for additional information Only those products bearing the UL Listing Mark for the US and Canada should be considered as being covered by UL s Listing and Follow Up Service meeting the appropriate requirements for US and Canada The UL Listing Mark for the US and Canada generally includes the UL in a circle symbol with C and US identifiers c 5 the word LISTED a control number may be alphanumeric assigned by UL and the product category name product identifier as indicated in the appropriate UL Directory Look for the UL Listing Mark on the product William R Carney Director North American Certification Programs UL LLC Any information and documentation involving UL Mark services are provided on behalf of UL LLC UL or any authorized licensee of UL For questions please contact a local UL Customer Service Representative at www ul com contactus Page 1 of 1 CERTIFICATE OF COMPLIANCE Certificate Number Report Reference Issue Date Issued to This is to certify that representative samples of Standard s for Safety Additional Information 20120416 E215059 E215059 2002 August 27 2012 Apr
6. can swing freely The aerosol tight buckets must not be stored with their caps sealed 4 3 Refrigerated centrifuges Clean condensation water and ice buildup regularly from the rotor chamber by defrosting using a soft absorbent towel Regularly empty and clean the condensation water collector Remove this from the left Please clean the condensation water drain regularly 4 Maintenance and cleaning 4 4 Glass breakage When centrifuging glass tubes please observe that high speeds rcf s increase the risk of glass breakage Please follow the manufacturer s instructions concerning the maximum speed rcf of centrifuge tubes In the event of glass breakage carefully remove all splinters and all ground glass from the rotor the buckets the adapters and the rotor chamber You may need to replace the rubber mats and adapters to prevent further damage Fine splinters of glass may otherwise scratch the surface of the rotors and buckets reducing their resistance to chemicals The air turbulences within the rotor chamber produce a very fine black powder of abraded metal In addition to causing damage to the rotor chamber rotor buckets and adapters the powder also contaminates the samples Check the rotor bores regularly for residues and damage 4 5 Returning devices When returning centrifuges please ensure that these devices are fully decontaminated so that they do not present a health risk to our service staff You will find addition
7. ee ee a ected Y 33 Activating and deactivating the signal tone 0 0 0 eect I 33 Parameter loCk eg ax pe ER Ex Sad pea Daas hea be Ema eee eh bah Sow ow Ree EBRURENEENE Ad Ms 33 Programming for 5702 R 5702 RH only 0 eens 34 Program selection for 5702 R 5702 RH only eh 34 Refrigeration for 5702 R only 0 ccc e ru mr 34 Heating and refrigeration only for 5702 RH 0 eee e rennen 35 Opening the centrifuge in case of power failure lllleeelele III 35 DevicefUses u ee ee ee 35 Maintenance and cleaning sareoeenne eee ee hh hh nnn 36 DEVICE oio a Edt wh bare doe eb oe odd die da beech bd Stee baht ober Ob eger obedece ibs 36 ROTOS MP EDITT 36 Refrigerated centrifuges eee lh m m Ih nn 36 Glass breakages eec 4222 denne ae deeds ee lab EEG Arcu peg bares 37 Returning devices sui unse een e EET REPERI are 37 Troubleshooting guide 0 00 cece eee ee hh hh hh hm hh nnn 38 Technical data ew csi ae tresta nEs CERO a CR REC RU CE RO CR RR a 39 Ordering information zu esc xe mm beu na weed eee ented E DR EEG GER eee iram Edu 40 1 Introduction Centrifuge 5702 is non refrigerated Centrifuge 5702 R is refrigerated and Centrifuge 5702 RH is a refrigerated and heatable bench top centrifuge For these centrifuges following rotors are available Fixed angle rotor F 35 30 17 with a capacity of 30 x 15 ml centrifuge tubes Fixed angle rotor F 45 24 11 w
8. fast temp Controlled temperature button prog 1 Program button 1 prog 2 Program button 2 C Condensation water tray 3 2 Fitting and removing the rotors When fitting the rotor follow the marking triangles on each side of the rotor nut They indicate the direction of the groove on the underside of the rotor and are needed to position the rotor safely To insert the rotor rotate the driver pin of the motor shift longitudinally Fit the rotor onto the motor shaft so the marking triangles point in the same direction as the motor shaft driver pin Tighten the rotor nut by turning them clockwise using the rotor key supplied If the rotor nut does not tighten easily check whether the rotor has caught on the motor shaft driver pin To release the rotor turn the rotor nut anticlockwise using the rotor key 3 3 Loading the rotors The rotors and buckets round and rectangular must be loaded symmetrically The adapters must be loaded only with the specified tubes Minimize the difference in weight between the filled sample tubes This will reduce wear on the drive and cut running noise The centrifuges 5702 5702 R 5702 RH have an automatic imbalance detector which shuts down the centrifuge if the weight differences are excessive This is indicated by the error message Inb in the display UN The maximum weight for a fully loaded bucket is indicated on each rotor 3 Operation 3 4 Fixed angle rotor The fixed angle rotor F 35 30 1
9. holders and adapters should be cleaned once a month or when necessary with a neutral cleaning agent to prevent residues of the material being centrifuged from changing the properties of the centrifuge and its accessories The rotor must be taken off for this Never place components in disinfectants or cleaning agents which contain sodium hypochlorite chlorine or are oxidants This will cause the material to change Disinfection with glutaraldehyde solution is possible We recommend Cidex activated glutaraldehyde solution The plastic adapters and rubber plates are dishwasher safe Check the tube holders and buckets for residues and corrosion For thorough cleaning remove the rubber plates from tube holders and buckets and clean all parts separately Then refit the rotor and bolt it into place with the supplied rotor key Check the rotor tube holders and buckets once a month for mechanical damage All rotors buckets adapters caps and tube holders can be autoclaved 121 C 20 min The aerosol tight cap including silicon sealing ring rubber mats and rectangular bucket adapters are subject to normal wear and must be replaced when a visual inspection reveals wear Do not unbolt the rectangular bucket adapters from each other On the swing bucket rotor make sure in particular that the pegs and grooves of the buckets are free of dirt They should be lightly lubricated with pivot grease provided with each swing bucket rotor so the buckets
10. lid open to toggle between b on signal tone on and b OF signal tone off Press the OPEN button for less than 2 seconds with the centrifuge lid open to display the current status With the Centrifuge 5702 R 5702 RH the selected signal tone is symbolized by the pictogram qd The current status is shown when the OPEN button is pressed for less than 2 seconds while the centrifuge lid is open Centrifuge 5702 3 15 Parameter lock To secure the centrifuge parameters against unintentional adjustment the Centrifuge 5702 and the Centrifuge 5702 R 5702 RH allows you to lock in the parameters you need Press and hold down OPEN and SHORT button simultaneously for at least 5 seconds with the centrifuge lid open After 5 seconds the dial adjuster is disabled and the centrifugation parameters are locked against unintentional adjustment Lo on appears in the display of the Centrifuge 5702 In the display of the Centrifuge 5702 R 5702 RH the locked parameters are symbolized by an additional pictogram The preset parameters can then no longer be altered whether the centrifuge is running or not The START STOP button can still be used to start and stop the centrifuge however It is also still possible to switch the signal tone and rcf switch on and off With the Centrifuge 5702 R 5702 RH it is also possible to additionally lock a program called up against unintentional resetting A program already written is called up through the program but
11. relevant national regulations Bioseals are a part of biological safety systems which are not able to guarantee the safety of people and the environment on their own when handling pathogenic microorganisms When working with pathogenic organisms of a higher risk group more than one aerosol tight bioseal must be provided for If the named liquids are spilled in the rotor or rotor chamber the centrifuge must be thoroughly and professionally cleaned Before using any cleaning or decontamination method other than that set out in section 4 Maintenance and cleaning please consult Eppendorf to ensure the intended method will not damage the device Rotors must always be properly secured The centrifuge may only be operated with the rotor firmly tightened For mechanical stability all the places on the rotor must be fitted with identical buckets The rotor may only be loaded symmetrically Opposing tubes should be of the same type and be filled equally On the rotor you will find information concerning the weight that a completely filled bucket may not exceed Prior to centrifugation the tubes should in any case be visually inspected for material damage Damaged tubes may not be centrifuged This is because broken tubes can in addition to sample loss result in further damage to the centrifuge and accessories Rotors showing clear signs of corrosion or mechanical damage must not be used Check the accessories regularly Rotors are high grade compone
12. ring The round bucket and the aerosol tight cap are autoclavable 121 C 20 minutes The aerosol tight cap including the silicone sealing ring is subject to natural wear and must be replaced when visual inspection reveals wear When handling the aerosol tight cap remember that polycarbonate has limited chemical resistance to phenol 3 7 Centrifugation with timer setting Switch on the centrifuge with the mains power switch if necessary and then with the switch The nominal values of the last run are displayed Load the rotor symmetrically and close down the centrifuge lid time Alters the running time speed Alters the speed in increments of 100 1 min or rcf start Starts the run The lii symbol flashes while the rotor is running stop Stops the centrifuge The 4 symbol appears briefly as soon as the rotor comes to a standstill stand by Centrifuge switched to stand by mode Open Releases the lid latch temp Changes the nominal temperature value for 5702 R 5702 RH only While the centrifuge is running the remaining time is displayed The last minute is counted down in seconds When the preset time elapses the centrifuge stops automatically and emits a signal tone to indicate the centrifugation is finished The lid of the non refrigerated Centrifuge 5702 opens automatically following centrifugation The lid of the refrigerated Centrifuge 5702 R 5702 RH remains closed to maintain the sample te
13. 004 5702 722 006 5702 761 001 5702 721 000 7 Ordering information Adapter for standard tubes and blood sampling systems for round bucket 85 ml 2 adapters 4x 1 5 2 0 ml micro test tubes 2 adapters 5x 1 1 1 4 ml adapter bore x max vessel length in mm 8 5 x 100 2 adapters 5x 2 7 ml 12 5 x 100 2adapters 4x 2 6 7 ml 13 5 x 100 4 adapters 4x 4 10 ml 16 x100 9 adapters 4x 9 15ml 17 5 x 100 2 adapters 1 x 15 ml Falcon 17 2 x 121 2 adapters 2 x 15 ml Falcon 17 2 x 121 2 adapters 1 x 25 ml 25 x100 2 adapters 1 x 50 ml Falcon 80 x115 2 adapters 1 x 85 ml 38 x106 Rectangular bucket 90 ml for A 4 38 set of 4 Rectangular bucket 90 ml for A 4 38 set of 2 Do not use with aerosol tight caps Adapter for standard tubes for rectangular buckets 2 adapters 10x 5 7ml 2adapters 8x 9ml 2 adapters 6 x 15 ml 2 adapters 4 x 20 ml 2 adapters 2 x 25 ml Swing bucket rotor type A 8 17 Adapter conical to support 15 ml Falcons for rotor A 8 17 set of 8 Rubber pad for 15 ml round bottom centrifuge tubes set of 8 Set of fuses for 5702 5702 R 230 V 2 x 2 5 A time lag for 5702 5702 R 120 V 2x 5 A time lag Important note 5702 745 006 5702 736 007 5702 737 003 5702 719 005 5702 735 000 5702 724 009 5702 732 001 5702 723 002 5702 717 002 5702 734 004 5702 718 009 5702 709 000 5702 762 008 5702 710 008 5702 711 004 5702 712 000 5702 713 007 5702 716 006 5702
14. 7 can be loaded with 15 ml Falcons and 15 ml round bottom centrifuge tubes It is available in the 10 and 30 position versions Please use only swing bucket rotor A 4 38 with round buckets and the appropriate adapters for centrifuging blood sampling systems Before centrifuging the Falcons place their plastic adapters in the tube holders The adapters prevent the Falcons from being destroyed For the centrifugation of round bottom tubes made of Duran glass round rubber pads are required to prevent the tubes from cracking Make sure a rubber pad is placed under each glass tube before centrifuging In order to avoid breakage of the tubes when using centrifuge tubes of glass polystyrene or similar materials please observe the limiting data with reference to endurance specified by the manufacturer The maximum load capacity for Falcons is 20 x 15 ml for 15 ml round bottom centrifuge tubes 30 x 15 ml The rotor can be loaded with a combination of 15 ml Falcons and 15 ml round bottom centrifuge tubes The maximum capacity is then 20 x 15 ml Falcons and 10 x 15 ml round bottom centrifuge tubes 15 ml round bottom centrifuge tube iy IK re Oy I 15 ml Falcon ZEN c Fig 3 Loading of rotor F 35 30 17 with 15 ml Falcons and 15 ml round bottom centrifuge tubes The specified max weight of 56 g imprinted on the rotor is the gross weight rating including the tube holder adapter tubes and cont
15. 700 002 5702 702 005 5702 701 009 5425 351 003 5425 353 006 Please use the original accessories recommended by Eppendorf Using spare parts or disposables which we have not recommended can reduce the precision accuracy and life of the centrifuges We do not honor any warranty or accept any responsibility for damage resulting from such action Falcon Registered trademark of Becton Dickinson Cidex Registered trademark of Johnson amp Johnson Duran Registered trademark of Schott Ficoll Registered trademark of Pharmacia Biotech AB Microtainer Registered trademark of Becton Dickinson Rotor code Fixed angle Angle of rotor adapter bore All Eppendorf rotors are designated and letters e g Aerosol tight version I I I according to a simple logical system which describes the technical specifi Ea 45 gu 1 1 cations as a uniform series of numbers I I Max no tubes adapters tube adapter Swing bucket rotor bore I I I Max no tubes adapters 41 M uoneuuojur Buuosp4o EG Konformit tserkl rung EC Conformity Declaration Das bezeichnete Produkt entspricht den einschl gigen grundlegenden Anforderungen der aufgef hrten EG Richtlinien und Normen Bei einer nicht mit uns abgestimmten nderung des Produktes oder einer nicht bestimmungsgem en Anwendung verliert diese Erkl rung ihre G ltigkeit The product named below fulfills the relevant fundamental requirements of the EC directives an
16. C UL or any authorized licensee of UL For questions please contact a local UL Customer Service Representative at www ul com contactus Page 1 of 1 Operatin i erating Manua f Evaluate your operating manual www eppendorf com manualfeedback eppendorf Your local distributor www eppendorf com worldwide Eppendorf AG 22331 Hamburg Germany Tel 49 40 53801 0 Fax 49 40 538 01 556 E mail eppendorf eppendorf com Eppendorf North America Inc 102 Motor Parkway Hauppauge N Y 11788 5178 USA Tel 1 516 334 7500 Toll free phone 1 800 645 3050 Fax 1 516 334 7506 E mail info eppendorf com Application Support Europe Tel 49 1803 666 789 Preis je nach Tarif im Ausland 9 ct min aus dem dt Festnetz Mobilfunkh chstpreis 42 ct min support eppendorf com North America Tel 1 800 645 3050 E mail techserv eppendorf com Asia Pacific Tel 60 3 8023 6869 E mail support_asiapacific eppendorf com 5702 901 016 10 052012 Printed in Germany Printed on chlorine free bleached paper eppendorf is a registered trademark
17. adhering impurities causing corrosion If material hazardous to health or aggressive material contaminate the device the owner is responsible for appropriate cleaning and decontamination Before cleaning unplug the power plug with the lid open unscrew the rotor using the rotor key supplied and clean it separately Use a mild cleaning agent for cleaning the accessible surfaces of the device Choose disinfection methods that correspond to the current legal regulations and guidelines for your area of application Use for example alcohol ethanol isopropanol or alcohol based disinfectants Do not allow any liquid to get into the gap at the motor shaft outlet For this reason the rotor chamber should be cleaned only with a damp cloth You should also consult your laboratory safety officer with regard to a suitable method of cleaning and disinfecting Before any cleaning or disinfecting method other than that recommended by the manufacturer is used please check with Eppendorf that the intended method will not damage the device or its accessories In order to ensure long term reliable work with your centrifuge please note that aggressive chemicals may damage the rotor and the chamber Check your device once a month for corrosion and damage The rubber seals in the rotor chamber should be rinsed off thoroughly with water and lubricated with glycerin or talc after every clean to prevent them becoming brittle 4 2 Rotors The rotors buckets tube
18. al information and a blank of the decontamination confirmation at www eppendorf com Do also consult your laboratory safety officer about a suitable decontamination method Please fill out the decontamination confirmation and place it together with the device when it is to be sent back to Eppendorf 37 Buuesp pue Dueus uley Troubleshooting 38 5 Troubleshooting Error Cause Remedy Stand by button lit red Centrifuge not ready Press STANDBY button No display No power Power failure Plug power cable in on both ends Check power fusing of lab device Lid will not open Power failure Rotor still running Emergency lid release see section 3 20 Wait for rotor to stop Centrifuge shakes when starting up Rotor unevenly loaded Stop centrifuge and load evenly Centrifuge will not start Lid not closed Press lid down or press OPEN button Display LID Lid not released Press lid down tight press Start Stop or emergency lid release Lid not locked Close lid again Lo on Centrifugation parameters With open centrifuge lid simultaneously press SHORT only 5702 locked against adjustment and OPEN for 5 seconds see 3 15 if parameters should be readjusted P 1 Program 1 or 2 is called up Press illuminated program button to exit the program P2 level if required FA Display indicates a FAST TEMP The run can be i
19. d buckets ro A8 Swing bucket rotor A 8 17 ro ALL General temperature profile for all rotors After 5 seconds a selected temperature profile will be automatically adopted and the display will return to the operating status temp The temperature nominal value can be set using the arrow keys During the rest period of the centrifuge the current temperature and the temperature nominal value are displayed alternately current temperature short temperature nominal value long Only the current temperature is displayed during the run A deviation of the set temperature nominal value of more than 3 C is signalled during the run by a blinking temperature display fast temp Starts a temperature control run in order to reach the preselected temperature as quickly as possible During the temperature control run FA appears in the display as well as the current temperature and the optimum rotational speed Refrigeration runs at less than room temperature are automatically carried out with a rotor specific low rotational speed while heating runs are carried out initially with a maximum and then with a nominal rotational speed For runs with precisely controlled temperatures we recommend starting a short Fast Temp run immediately prior to centrifugation This prevents an overshooting of the temperature in the rotor chamber such as can sometimes occur after longer standby periods Stand by The rotor chamber is heated or cooled to the selected n
20. d standards listed In the case of unauthorized modifications to the product or an unintended use this declaration becomes invalid Produktbezeichnung Product name Centrifuge 5702 5702 R 5702 RH einschlie lich Zubeh r including accessories Produkttyp Product type Laborzentrifuge Laboratory Centrifuge Einschlagige EG Richtlinien Normen Relevant EC directives standards 2006 95 EG EN 61010 1 EN 61010 2 20 2004 108 EG EN 55011 B EN 61000 6 1 EN 61000 3 2 EN 61000 3 3 EN 61326 98 37 EG EN 292 2 EN 292 2 A1 98 79 EG EN 14971 EN 61010 2 101 s VorstandfBoard of Management Projektmanagement Project Management 21 05 2007 Hamburg Date so 900 eppendorf Eppendorf AG Barkhausenweg 1 22339 Hamburg Germany 0015 033 509 02 5703 900 307 04 CERTIFICATE OF COMPLIANCE Certificate Number Report Reference Issue Date Issued to This is to certify that representative samples of Standard s for Safety Additional Information 20120416 E215059 E215059 2002 February 28 2012 April 16 EPPENDORF ZENTRIFUGEN GMBH RIESAER STR 198 04319 LEIPZIG GERMANY Laboratory use Electrical Equipment Centrifuge Model 5702 Have been investigated by UL in accordance with the Standard s indicated on this Certificate ANSI UL 61010 1 Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements and C22 2 No 1010 1 1992
21. device can lead to back injuries The device may only be transported in its original packaging The device must be transported by least two people Use a transport aid e g dolly to transport the device longer distances For 5702 R RH only In order to prevent damage to the compressor following improper transport the device may not be switched on until four hours after setting up For 5702 only Please remove the transport safety device and keep it for possible use if the device is subsequently moved again Place the centrifuge on a solid flat non resonant lab bench For the Centrifuge 5702 R RH the condensation water tray should be inserted from the side see cover flap The surrounding area must be well ventilated and protected against direct sunlight To ensure that the ventilation of the device is not impaired a fundamental clearance of 30 cm to the side and a min of 15 cm to the back wall must be maintained This is especially important for the refrigeration capacity of the 5702 R RH During centrifugation according to the recommendations set out in EN 61010 2 020 a safety clearance of 30 cm must be maintained around the centrifuge within which there are no objects which may be destroyed and so cause further damage Only connect the device to power supplies which correspond with the electrical requirements on the name plate Only use sockets with a protective earth PE conductor and suitabl
22. e power cable Now connect the centrifuge to the power supply and switch it on at the main power switch on the rear side see cover flap The centrifuge is now ready for operation The Stand by button lights up green and the display is active Before starting check that the rotor is firmly seated 25 uononpouu Safety precautions and applicational limitations 26 2 Safety precautions and applicational limitations N For your personal safety please be sure to comply with the following regulations unconditionally The centrifuge 5702 5702 R 5702 RH must only be use for the specified applications see Introduction It must not be operated in explosive atmospheres Explosive or highly reactive substances must not be centrifuged When being moved from the cool room to a normal lab environment the centrifuge must either warm up for half an hour in the cool room first or it must warm up for at least 3 hours in the lab before being connected to the supply system in order to prevent damage by condensation The centrifuge must not be moved or knocked while in operation Improperly installed or serviced centrifuges must not be operated Repairs may only be carried out by Service personnel authorized by Eppendorf Use only original Eppendorf spare parts and rotors When handling toxic radioactive liquids or pathogenic microorganisms of risk group Il see World Health Organization Laboratory Biosafety Manual comply with the
23. ents Always weigh the tube holder and the load together A maximum of 24 1 5 2 0 ml test tubes can be centrifuged in rotor F 45 24 11 If the appropriate adapters are used it is also possible to load it with 0 2 ml PCR tubes 0 4 ml test tubes 0 5 ml test tubes and 0 6 ml Microtainers Fixed angle rotor F 45 18 17 Cryo can be equipped with cryotubes max diameter 17 mm or 13 mm with adapters and sealable centrifuge tubes max diameter 16 5 mm or 12 2 mm with adapters In each of these cases max tube length is 50 mm Maximum load adapter tube and contents per bore is 3 75 g on rotor F 45 24 11 and 8 7 g on rotor F 45 18 17 Cryo When loading the rotors please make sure that the tubes are inserted in the bores of the rotor opposite one another in pairs To ensure that the rotor is loaded symmetrically the tubes opposite one another need to contain approximately the same filling quantity Fixed angle rotors are operated without a rotor lid 29 uone42do Operation 30 3 Operation 3 5 Swing bucket rotors The swing bucket rotor A 4 38 can be equipped with round or rectangular buckets Round buckets and the associated adapters see ordering information are conceived for the centrifugation of Falcons blood withdrawal systems and other centrifuging tubes with round bottoms Rectangular buckets and associated adapters see ordering information are conceived for the centrifugation of glass centrifugation tubes UN Use onl
24. evel Overvoltage category Degree of contamination Technical specifications subject to change Centrifuge 5702 230 V 50 60 Hz 120 V 50 60 Hz 100 V 50 60 Hz 200 W 4 400 rpm 3 000 rcf 4 x 90 ml 2 280 Nm 1 2 g ml 2 40 C 75 96 max 2000 m above NSL Height 241 mm Depth 395 mm Width 320 mm 20 kg 25 sec 25 sec 25 sec 25 sec 2 5 A time lag 230 V 5 0 A time lag 120 V 100 V 58 dB A Centrifuge 5702 R 5702 RH 230 V 50 60 Hz 120 V 50 60 Hz 100 V 50 60 Hz 380 W 4 400 rpm 3 000 rcf 4 x 90 ml 2 280 Nm 1 2 g ml 10 40 C 75 96 max 2000 m above NSL Height 265 mm Depth 580 mm Width 380 mm 36 kg 25 sec app 25 sec 25 sec app 25 sec 2 5 A time lag 230 V 5 0 A time lag 120 V 6 0 A time lag 100 V 54 dB A steady state at 4 C 23 C ambient temperature empty rotor II 2 39 ejep e31uupa Ordering information 40 7 Ordering information Centrifuge 5702 230 V 50 60 Hz without rotor Centrifuge 5702 R 230 V 50 60 Hz without rotor Centrifuge 5702 RH 230 V 50 60 Hz without rotor Fixed angle rotor and accessories Fixed angle rotor F 35 30 17 Aluminum 30 places angle 35 for mounting of 15 ml Falcons or 15 ml round bottom centrifuge tubes complete with stainless steel tube holders for 30 places 10 places Tube holders stainless steel for rotor F 35 30 17 for mounting
25. il 16 EPPENDORF ZENTRIFUGEN GMBH RIESAER STR 198 04319 LEIPZIG GERMANY Laboratory use Electrical Equipment Centrifuge Model 5702R 5703 and 5702RH 5704 Have been investigated by UL in accordance with the Standard s indicated on this Certificate ANSI UL 61010 1 Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements and C22 2 No 1010 1 1992 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part General Requirements Part 2 Particular Requirements for Laboratory Centrifuges CAN CSA C22 2 No 1010 2 020 92 CAN CSA C22 2 No 1010 2 020A 97 See the UL Online Certifications Directory at www ul com database for additional information Only those products bearing the UL Listing Mark for the US and Canada should be considered as being covered by UL s Listing and Follow Up Service meeting the appropriate requirements for US and Canada The UL Listing Mark for the US and Canada generally includes the UL in a circle symbol with C and US identifiers c 5 the word LISTED a control number m ay be alphanumeric assigned by UL and the product category name product identifier as indicated in the appropriate UL Directory Look for the UL Listing Mark on the product William R Carney Director North American Certification Programs UL LLC Any information and documentation involving UL Mark services are provided on behalf of UL LL
26. imprinted on the rotor is the gross weight rating of a bucket including adapter tubes and contents The maximum load adapter tubes and contents of the round bucket is 190 g The maximum load adapter tubes and contents of the rectangular bucket is 240 g A maximum of 8 x 15 ml Falcons or 8 x 15 ml tubes of Duran glass can be centrifuged in the rotor A 8 17 Prior to the centrifugation of 15 ml Falcons please insert the provided plastic adapter into the rotor Prior to the centrifugation of tubes of Duran glass please insert the round rubber plate into the rotor Please make certain prior to centrifugation that such a rubber plate is found under every glass tube In order to avoid breakage of the tubes when using centrifuge tubes of glass polystyrene or similar materials please observe the limiting data with reference to endurance specified by the manufacturer Only load the rotor symmetrically so that the rotor and the pivots are evenly loaded during centrifugation The maximum load adapter tubes and contents of the rotor A 8 17 is 8 x 38 g The maximum permissible tube length is 120 mm 3 Operation 3 6 Operation of round bucket with aerosol tight caps The inner part of the aerosol tight cap is also equipped with a silicone sealing ring Never remove this sealing ring from the cap Whenever using the cap make sure that the sealing ring is undamaged and sits uniformly in the groove Silicone sealing
27. ions within the EU may vary from country to country please contact your supplier if necessary Declaration concerning the ATEX directive 94 9 EC The present design and ambient conditions inside Eppendorf centrifuges mean that they are not suitable for use ina potentially explosive atmosphere The centrifuges must therefore only be used in a safe environment such as the open environment of a ventilated laboratory or a fully extracted fume hood The use of substances which may contribute to a potentially explosive atmosphere is not permitted The final decision on the risks in connection with the use of such substances is the responsibility of the user of the centrifuge 27 N suoyeyuj Jeuoneordde pue suonneoa4d HLS Operation 28 3 Operation 3 1 Functional and operator control elements Refer to the frontal view on the first inside cover page of this manual Fig 1 Front view of the Centrifuge 5702 Fig 2 Front view of the Centrifuge 5702 R Front of the Centrifuge 5702 RH is structurally identical to that of the 5702 R time Time dial and Start Stop button short Short run button speed Speed selector dial and rpm rcf button 6 Stand by button open Lid release 1 Line socket with fuse set and main power switch device identification plate with power supply ratings rear E Emergency lid release on underside with pull cord For the Centrifuge 5702 R 5702 RH only temp Temperature selection buttons
28. ith a max capacity of 24 x 1 5 2 0 ml in micro test tubes Fixed angle rotor F 45 18 17 Cryo with a max capacity of 18 x cryotubes max 17 mm Swing bucket rotor A 4 38 with a max capacity of 4 x 85 ml in round buckets and 4 x 90 ml in rectangular buckets Swing bucket rotor A 8 17 with a max capacity of 8 x 15 ml Before using the centrifuge 5702 5702 R 5702 RH for the first time please read the operating manual The latest version of the manual and the safety instructions in your language can be found on the Internet at www eppendorf com You will see this symbol on your centrifuge and at a number of points throughout this manual The texts it highlights are relevant to safety Use the centrifuge only after having read the safety notices 1 1 Intended use The Centrifuge 5702 5702 R 5702 RH is intended exclusively for indoor use and for separating aqueous solutions and suspensions of various densities in approved test tubes This device may only be operated by trained specialist staff They must have carefully read the operating manual and be familiar with the function of the device 1 2 Delivery package 1 Centrifuge 5702 non refrigerated or 1 Centrifuge 5702 R refrigerated with condensation water tray or 1 Centrifuge 5702 RH refrigerated and heatable each without rotor 1 power cable 1 operating manual 1 rotor key 1 set of fuse 1 3 Installing the device AN The device is heavy Lifting and carrying the
29. kes the centrifuge accelerate and decelerate gently This guarantees optimal centrifugation results Press the SHORT key for longer than 5 seconds while the centrifuge lid is open On the Centrifuge 5702 the text in the display changes from br on brake on to br OF brake off The symbol appears in the display of Centrifuges 5702 R RH The slower acceleration and braking ramps are now activated The current status will be displayed when the SHORT button is pressed for less than 5 seconds while the centrifuge lid is open To switch faster acceleration and braking back on press the short key for longer than 5 seconds again while the centrifuge lid is open The br on message appearing briefly in the display signals the reactivation of the faster acceleration and braking ramps 3 13 At set rpm For centrifugation time can be counted either immediately from the start or not until attainment of the preset speed Pressing the START STOP button for longer than 2 seconds with the centrifuge lid open switches the at set rom mode symbolized by the pictogram To exit the at set rom mode again and begin counting down the centrifugation time immediately after starting the centrifuge press the START STOP button again for longer than 2 seconds with the centrifuge lid open until the pictogram Z is displayed 3 14 Activating and deactivating the signal tone Press and hold down the OPEN button for longer than 2 seconds with the centrifuge
30. mperature and can be opened with the OPEN button When you switch off the centrifuge 5702 5702 R 5702 RH at the main power switch the display goes blank after a few seconds delay 3 8 Changing the centrifugation parameters during the run The timer setting the rotation speed and the temperature only 5702 R 5702 RH can be changed during the run by briefly pressing the SHORT button The display begins flashing You can then set the new centrifugation parameters with the TIME and SPEED and the TEMP dial adjustors The new centrifugation parameters are programmed after 5 seconds 3 9 Short centrifugation short Press this button with the lid closed to start a short run at maximum speed The centrifuge stops when you release the SHORT button again 31 uogeisdg Operation 32 3 Operation 3 10 Continuous running time The continuous run function is set by turning the TIME dial to either above 99 minutes or below 0 5 minutes The timer displays oo to indicate that continuous running is active The time is counted upward in minutes stop Ends continuous running 3 11 Rcf display and calculation SPEED Press the dial to toggle the display between 1 min rpm and rcf and vice versa Note that the rcf g force indicated when the display is switched is standardized to 15 ml Falcon tubes in the rotor A 4 38 At 4 400 1 min you can achieve the following maximum rcf with the various adapters
31. ned the refrigeration switches off to protect the device Remark In cases of higher ambient temperature temporary air noises may occur until the desired temperature is attained These are a sign of high refrigeration capacity Block the ventilation slots under no circumstances For ambient temperatures below 18 C a warming up time of 1 hour is required before operating 3 Operation 3 19 Heating and refrigeration 5702 RH In the case of the Centrifuge 5702 RH we are dealing with a benchtop centrifuge with a regulated heating and cooling system The Centrifuge 5702 RH can be refrigerated or heated precisely within a temperature range from 9 C to 42 C By way of preprogrammed temperature profiles it is possible to set optimized temperature arcs for the available swing bucket and fixed angle rotors This enables exact temperature control even for sensitive samples Setting the preprogrammed temperature profiles Pressing the Fast Temp key while the centrifuge lid is open results in the display of the set rotor with the corresponding temperature profile Using the arrow keys it is possible to select other temperature profiles The displays appearing stand respectively for the following rotors Display Rotor ro F 35 Fixed angle rotor F 35 30 17 ro F 24 Fixed angle rotor F 45 24 11 ro F 18 Fixed angle rotor F 45 18 17 Cryo ro A4 rE Swing bucket rotor A 4 38 with rectangular buckets ro A4 ro Swing bucket rotor A 4 38 with roun
32. nterrupted with the START STOP run button if required Inb Rotor unevenly Check loading and repeat run loaded Int Power failure during run Check power plug Wait for rotor to come to a stop Repeat run Er2 Unbalanced rotor switch Inform Service defective Er 3 Error in speed system Leave device switched on until error message disappears up to 5 min Er 5 Lid switch Close lid restart Er 6 Drive error Repeat run Er 7 Overspeed Error in drive or speed measurement system Er 8 Drive error Repeat run Er 9 25 Electronics error Repeat run Er 18 Temperature deviation Nominal value setting too low or refrigeration defective 5 C fr nominal value Er 19 Refrigeration unit overheated Make sure that the air circulation through the cooling slots is not impaired Er 23 Motor overheating Allow motor to cool only 5702 Er 24 Fault in the refrigeration unit At start of operation contact Service After longer running time allow centrifuge to cool down If the suggested remedy repeatedly fails please contact Service 6 Technical data Line connection Power output Max speed Max centrifugal acceleration Max load Max kinetic energy Permissible density of material being centrifuged Ambient temperature Max rel air humidity Positioning height Dimensions Weight excluding rotor Startup time 230 V Deceleration time Startup time 100 V 120 V Deceleration time Fuses Noise l
33. nts which have to withstand extreme stresses and strains Aluminum rotors are largely protected from corrosion by the most common laboratory chemicals by means of an anodized coating though the protection is not unlimited Protect the rotors from mechanical damage Even minor scratches or cracks can result in serious internal material damage Avoid damaging the rotors by the use of aggressive chemicals such as strong and weak alkalis strong acids solutions of mercury copper and other heavy metal ions chlorinated hydrocarbons concentrated salt solutions and phenol If the rotor is contaminated by aggressive substances clean it immediately with a mild cleaning agent The material being centrifuged may not exceed a density of 1 2 g ml at maximum rotation speed If the rotor is run for a lengthy period of time or more often with short centrifugation runs the sample tubes will become hot Keep within the limits specified by the tube manufacturers Seal the tube lid down tight before centrifuging The lids of unclosed tubes may rip off during centrifugation and damage the centrifuge When using organic solvents e g phenol chloroform the durability of plastic tubes may be impaired When closing the centrifuge lid do not place your fingers between the lid and the centrifuge otherwise they may be trapped 2 Safety precautions and applicational limitations The following rotors and accessory buckets have a maximum service life of 7 years
34. of 15 ml Falcons or 15 ml round bottom centrifuge tubes set of 10 Adapter conical to support 15 ml Falcons for rotor F 35 30 17 set of 10 Rubber mat for 15 ml round bottom centrifuge tubes set of 10 Fixed angle rotor F 45 24 11 Aluminum angle 45 24 places max 11 mm for mounting of 1 5 2 0 ml micro test tubes Adapter for 0 2 ml PCR tubes for rotor F 45 24 11 set of 6 Adapter for 0 4 ml micro test tubes for rotor F 45 24 11 set of 6 Adapter for 0 5 ml micro test tubes and 0 6 ml Microtainer for rotor F 45 24 11 set of 6 Fixed angle rotor F 45 18 17 Cryo Aluminum angle 45 18 places max 17 mm for mounting of cryotubes max 17 mm and centrifuge tubes which can be sealed max 16 5 mm max length in mm 50 Adapter for cryotubes max 13 mm and centrifuge tubes which can be sealed max 12 2 mm max length in mm 50 Swing bucket rotors and accessories Swing bucket rotor 4 x 85 ml type A 4 38 aluminum incl 4 round buckets Swing bucket rotor 4 x 85 ml type A 4 38 aluminum excluding round buckets Round bucket 85 ml for A 4 38 set of 4 Round bucket 85 ml for A 4 38 set of 2 Caps aerosol tight for round bucket 85 ml set of 2 5702 000 019 5703 000 012 5704 000 016 5702 704 008 5702 705 004 5702 707 007 5702 706 000 5702 708 003 5702 746 002 5425 715 005 5425 717 008 5425 716 001 5702 747 009 5702 752 002 5702 720 003 5702 740
35. ominal temperature both Heating and before and after a run with the lid closed Because the rotor does not thereby rotate refrigeration the temperature adjustment occurs slowly The current temperature in the rotor chamber is displayed For runs with precise temperature control following longer standby periods we recommend starting a brief Fast Temp run immediately prior to centrifugation This prevents potential overshooting of the temperature in the rotor chamber 3 20 Opening the centrifuge in case of power failure Pull the power plug and wait for the rotor to come to a stop this may take as long as 5 minutes Emergency lid release on underside of device by pull cord In front of the front right suction foot there is a small white plastic cap in the base plate see E in figures 1 and 2 cover flap Remove the cap and draw the cord out straight downward Please be sure to later make certain that the cord is pushed back completely into the housing before closing the lid Press the plastic button into the baseplate again 3 21 Device fuses The fuse box is located under the main power plug The fuse box can be removed from the rear The two fuses can be replaced see Ordering information 35 uogedo Maintenance and cleaning 36 4 Maintenance and cleaning 4 1 Device The outer surfaces of the centrifuge and the rotor chamber should be cleaned regularly with a mild cleaning agent This is for hygiene purposes and to prevent
36. tons and then as described above the program buttons and dial adjustor setting deactivated To enable the dial adjuster again press and hold down OPEN and SHORT button simultaneously for 5 seconds with the centrifuge lid open The display shows Lo OF or w 5702 R 5702 RH The current status Lo on can be displayed on the Centrifuge 5702 by briefly pressing the SHORT and OPEN buttons while the lid is open 33 uoneJ42dQo Operation 34 3 Operation 3 16 Programming for 5702 R 5702 RH only With the Centrifuge 5702 R 5702 RH it is possible to store up to two permanent programs only during standstill First set the required program data with the TIME and SPEED dial adjustors The function at set rpm and the switched off braking function can also be stored if necessary in the permanent program PROG 1 or 2 Hold down for 2 seconds until the program button is no longer flashing and lights up continuously A brief signal will sound The program is now stored 3 17 Program selection for 5702 R only Program selection is only possible during stillstand PROG 1 or 2 u Pressing once calls up the required program The button for the program activated lights up in blue If dial adjustors or buttons are required for the program setting the program remains unchanged The text Pr 1 Program 1 or Pr 2 Program 2 appears in the centrifuge display Exit the program by again pressing the program button The bl
37. ue lighting of the button extinguishes and the centrifugation parameters can be set freely again 3 18 Refrigeration for 5702 R only temp The nominal temperature value can be set with the arrow keys from 9 C to 40 C During stillstand the nominal temperature setting and the current temperature in the rotor chamber are displayed alternately With the centrifuge running only the current temperature in the rotor chamber is displayed A deviation of more than 3 C from the temperature set is signalled during the run fast temp starts a controlled temperature run with a centrifuge specific rotation speed in order to quickly reach the selected temperature in the rotor During the FAST TEMP run FA appears in the display as well as the present temperature and the fixed optimal rotational speed The run ends automatically or by pressing the STOP button A signal tone sounds periodically Stand by refrige With closed centrifuge lid the rotor chamber is refrigerated before and after a run to ration the selected nominal temperature if this is less than the ambient temperature Since there is no rotation of the rotor the temperature adaptation takes place slowly If the centrifuge was in standby refrigeration over a longer time or at low temperatures start a brief FAST TEMP run before inserting the samples in order to prevent the samples from freezing If the centrifuge is not used for more than 8 hours or the lid is not ope
38. y the rotor bucket round or rectangular adapter combinations approved by Eppendorf Check that all buckets are fully attached lubricated lightly and can swing out freely If you use overlength tubes it is essential to carry out a manual swing test with empty tubes When the rectangular buckets are not completely filled insert the adapters so that the bolts of the rotor A 4 38 are symmetrically loaded Avoid unbalanced loading of the inner adapter bores pointing to the rotor nut since this could cause overshooting of the rectangular buckets For mechanical stability all the places must be fitted with identical buckets The buckets are sorted by weight The weight class is embossed on the side of the bucket e g 90 the last 2 digits in grams Opposing buckets must be of the same weight class When reordering please quote the weight class required Before inserting the buckets in the grooves make sure the grooves are clean Dirty grooves or pegs will prevent the buckets from swinging out evenly Fig 4 Fig 5 Fig 6 l Rotor and buckets symmetrically Rotor and buckets asymmetrically Adapter loaded asymmetrically loaded loaded Not permitted because the Not permitted because the pegs of the bucket are not uniformly loaded and the loading of the adapter bores pointing to the rotor nut could cause overshooting of the rectangular buckets pegs of the buckets are subject to differing loads The specified max weight of 400 g
Download Pdf Manuals
Related Search