User Manual
Contents
1. Figure 3 With the Stabilizor T1 system ex vivo degradation is minimized which maximize your chance to find what you are really looking for instead of distorting noise from degraded proteins 6 Stabilizor T1 DPM 0008 Edition AC Introduction 1 For information on how the Stabilizor system can be utilized in different applications please visit Denator s website www denator com or contact Denator at application denator com 1 1 TERMINOLOGY DEFINITIONS EX vivo Ex vivo time Stabilization Sample Mock run Stabilizor T1 DPM 0008 Edition AC Events taking place outside the living organism Time that the sample has been outside the living organism The inactivation of degradation of proteins performed in Stabilizor T1 One sample from one individual A test run with blank samples to ensure that the sample collection procedure work smoothly denaltor 2 2 1 Safety information IMPORTANT USER INFORMATION 2 1 1 Intended use Stabilizor T1 and all associated products from Denator AB are for research use only Not for use in diagnostic procedures for clinical purposes 2 1 2 Warning caution and note definitions are not followed It is very important to address all safety issues stated in Warning A warning indicates that there is a risk of personal injury if the instructions AN Warnings instructions not are followed It is important to address all issues stated in D Caution A caution indicates that there is2. 7 mm is the maximum sample thickness 26 Stabilizor T1 DPM 0008 Edition AC Stabilizing samples 6 6 1 2 Starting the instrument Warning Ensure that all instrument site requirements including a grounded power outlet are fulfilled before starting the instrument see Section 3 2 1 Insert a USB flash drive for the log file in a USB port 2 Turn the instrument on using the power switch 3 The system check is automatically performed During the system check the illumination is first red while the sample tray is moved in and out and then changes to blue The heating blocks are heated to the set temperature Note The sample tray can move unexpectedly The system check and heating of the heating blocks will take 3 5 minutes depending on available voltage The main screen is displayed when the system check is finished Sample name NO NAMI Card number Card type Na Method Stabilizor T1 DPM 0008 Edition AC 27 denaltor 6 1 3 Preparing the Maintainor card 1 Take out a Maintainor card from its package 2 Mark the Maintainor card with sample ID and if desired a card ID of your own gt If several samples are to be stabilized it is recommended to mark all Maintainor cards before starting the sample collection 6 1 4 Instument preparations Enter settings for the run according to Section 4 3 on page 19 28 Stabilizor T1 DPM 0008 Edition AC Stabilizing samples 6 6 2 SAMPLE COLLECTION AND
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4. STABILIZATION Before starting ensure that e all preparations in Section 6 1 are made e the heating block temperature is correct normally 95 C e the sample source is available 6 2 1 Sample collection Use gloves when handling biological samples 1 Open the Maintainor card and place it according to the table below Sample type Recommendation Fresh Place the Maintainor card directly in the Stabilizor T1 before collecting the sample This will give the shortest ex vivo time Frozen Place the Maintainor card on ice to keep the sample cold before the stabilization Caution Do not put the Maintainor card into liquid nitrogen The Maintainor card withstands temperatures down to 80 Stabilizor T1 DPM 0008 Edition AC 29 Adenator 2 Start the measurement of the ex vivo time just prior to sampling by pressing the ex vivo button on the touch screen and immediately continue with step 3 3 Collect a sample of appropriate size 4 Put the sample in the center of the sample chamber of the Maintainor card Av 5 Close the Maintainor card by pressing the lid down Ensure that the lid has sealed completely by gently pressing down the cover lid encircling the sample chamber using one or two fingers c Caution Do not press the inner edge of the cavity The upper plastic can be damaged leading to leakage of air during the heating process 30 Stabilizor T1 DPM 0008 Edition AC Stabilizing samples 6 6 2 2 S
5. a risk of damaging the instrument if the Cautions in order to keep your equipment in good condition gt A note indicates important information for improving your results or facilitating your work 2 1 3 Recycling All electrical and electronic equipment must not be disposed as unsorted municipal bid waste and must be collected separately Please contact an authorized representative of the manufacturer for information EE concerning the decommissioning of equipment Stabilizor T1 DPM 0008 Edition AC Safety information 2 2 2 LIST OF WARNINGS AND CAUTIONS interference in which case the user might be required to take appropriate Warning This is a Class A product In a domestic environment it might cause radio A measures Warning No user serviceable paris inside All repairs should be done by personnel authorized by Denator Do not open any covers or replace parts unless specifically stated in the instructions Warning Do not block the rear panel of the instrument The power switch must always be easily accessible Warning Hot parts inside Do not remove the top cover unless the instrument has been turned off and allowed to cool down for 30 min Warning Hazardous voltage Stabilizor T1 contains mains voltage of up to 240 V ac Disconnect power cord before removing the top cover Warning Laser inside The instrument must always be turned off before the top cover is removed Warning Sharp needle To
6. extraction and inactiviation 10 min Figure 1 Effect of post mortem time delay on the number of detected peptides derived from mouse hypothalamus Three dimensional representations of LC MS detected peptides and protein fragments of the control group and 10 min post mortem group respectively Skold K 2007 The Stabilizor system consisting of the Stabilizor T1 instrument in combination with a Maintainor card Figure 2 has been developed to address the problem of ex vivo protein degradation Stabilizor T1 ensures irreversible heat inactivation to minimize ex vivo degradation Stabilization in the Stabilizor T1 system should not be used if biological activity is to be measured in the sample Skold K Svensson M et al 2007 Proteomics 7 4445 4456 Stabilizor T1 DPM 0008 Edition AC 5 denalor Figure 2 Stabilizor T1 Maintainor Tissue and Stabilizor USB flash drive Denator s method of stopping ex vivo degradation is based on rapid thermal inactivation of the sample The instrument is designed to ensure the highest possible reproducibility independent of the shape and size of the sample The sample card Maintainor facilitate rapid processing and efficient storage of the sample By using Denator s workflow for an efficient and reproducible stabilization of biological samples the number of downstream analysis artefacts is minimized Che palaria impara data berries Cocdiborn difficult to nandardizs
7. recommended to turn the instrument off after setting it into service mode Set the instrument to service mode when maintenance or cleaning is needed or if something is dropped into the instrument To set the instrument in Service mode 1 From the main screen press the OPTIONS button Sample name NONAME Card number Oo so Card type Tissue Method STF IRE 2 Press the SERVICE MODE button and follow the instructions on the screen Stabilizor T1 DPM 0008 Edition AC 17 denalor 4 2 3 Transport mode When the instrument needs to be moved Transport mode should be used To set the instrument in Transport mode 1 Place a closed empty Maintainor card on the sample tray 2 From the main screen press the OPTIONS button Sample name MO MAME Card number DODI Card type Method 3 Press the TRANSPORT MODE button 4 Follow the instructions on the screen 5 Move the instrument according to Section 3 4 Storage Stabilizor T1 should be stored in 10 to 45 C and max 85 relative humidity 18 Stabilizor T1 DPM 0008 Edition AC Software 4 4 3 STABILIZATION SETTINGS There are three settings to be entered for each sample before starting a stabilization e Sample name see Section 4 3 1 e Card number see Section 4 3 2 e Method see Section 4 3 3 Data is entered on the touch screen via the key pad available in the software To be able to enter data the Stabilizor T1 must be in running m
8. AC Stabilizor T1 instrument 3 3 2 INSTRUMENT SITE REQUIREMENTS gt Figure 6 Dimensions of Stabilizor T1 143 mm For proper operation Stabilizor T1 requires an ambient temperature of 10 to 40 C and 80 maximum relative humidity Make sure there is adequate air circulation around the instrument For indoor use only Warning Do not block the rear panel of the instrument The power switch must always be easily accessible Power requirements e Mains voltage 100 240 VAc auto range 50 60 Hz e Connect only to grounded power outlets e Power consumption 350 W max 70 W typical use e Usea slow 20x5 mm ceramic fuse T4A H that holds for 250V 3 3 INSTALLING THE INSTRUMENT 1 Unpack the instrument Place the instrument on an appropriate surface according to Section 3 2 Insert the power cord into the instrument and into a grounded power outlet Turn the instrument on using the power switch ao Km O N The instrument is now ready to use Stabilizor T1 DPM 0008 Edition AC 13 denalor 3 4 MOVING THE INSTRUMENT 14 o Caution The instrument can be damaged if the instructions for moving the instrument is not followed 1 Setthe instrument to transport mode see Section 4 2 3 Turn the instrument off using the power switch Remove the cord from the power socket Move the instrument to the new location Insert the cord into a grounded power outlet O oa Km W N Turn the instrument on using the
9. abilize the sample for further analysis To enhance the heat transfer vacuum is applied to the sample chamber before the heat stabilization Vacuum is also applied after the heat stabilization to remove the vapor formed during the heat stabilization The Maintainor card is for single use only 3 Figure 9 Main parts of Maintainor card 5 1 STORAGE 1 Cover lid 2 Sample chamber 033 mm 3 Labelling area for sample name additional information and or barcode 4 Air tight seal 5 Vacuum channel with valve 6 Guide slot Storage temperature Empty 10 to 50 C max relative humidity 85 non condensing With stabilized sample 80 C to 20 C Stabilizor T1 DPM 0008 Edition AC 29 denalor 6 Stabilizing samples 6 1 PREPARATIONS 6 1 1 Planning the experiment Plan the experiment with regards to factors like sample number identity tissue type thickness planned ex vivo time based on normal sample preparation times selected stabilization method to be used and Maintainor card type General recommendations e Make sure you have sufficient quantity of the required equipment and consumables e Plan your experiment to combine short ex vivo time and careful sample collection Work efficiently and fast but avoid stress during sample collection e Perform a mock run to ensure that you have everything you need and an efficient working process before starting with the real samples e For Maintainor Tissue
10. ample stabilization Immediately start the stabilization process 6 If not already done place the Maintainor card in the sample tray 7 Start the run by pressing the start button above the touch screen or by pressing Start on the touch screen Stabilizor T1 DPM 0008 Edition AC 31 denaltor 8 When the illumination is green and the sample tray is in the starting position again lift out the Maintainor card from the sample tray 9 Check that there is vacuum in the sample compartment no vacuum indicate need of needle device change or remounting of the needle device 10 When the stabilization is ready store the Maintainor card with stabilized sample at 80 C to 20 C for long term storage or prepare sample for analysis Caution Do not put the Maintainor card into liquid nitrogen The Maintainor card can only withstand temperatures down to 80C 11 The next sample if prepared can be stabilized as soon as the processed Maintainor card has been removed 6 3 LOG FILES Log files are automatically saved on the USB flash drive see Section 4 5 32 Stabilizor T1 DPM 0008 Edition AC Maintenance 7 7 Maintenance 7 1 MAINTENANCE AND SERVICE Service of Stabilizor T1 should be performed by service engineers from Denator Contact information to Denator is provided on the back cover The following maintenance can be made by the customer e Changing the O ring including cleaning heat blocks see Section 7 2 e Changing
11. avoid injury take care when handling the needle device Warning Ensure that all instrument site requirements are fulfilled before starting the instrument Caution Handle the needle device carefully to avoid bending the needle Caution The instrument can be damaged if the instructions for moving the instrument are not followed Caution Do not put the Maintainor card into liquid nitrogen The Maintainor card withstands temperatures down to 80 OOOBPBBBBBB Stabilizor T1 DPM 0008 Edition AC 9 Jdenalor 3 Stabilizor T1 instrument 3 1 MAIN PARTS 8 9 10 6 Figure 4 Main parts of Stabilizor T1 No Instrument part No Instrument part 1 Touch screen Ex vivo button 2 Start buttons 7 Top cover 3 Sample tray 8 Needle device 4 USB ports 9 Needle holder 5 Illumination 10 Needle guide 10 Stabilizor T1 DPM 0008 Edition AC Stabilizor T1 instrument 3 14 15 Figure 5 Main parts of Stabilizor T1 lcd Instrument part No Instrument part Power switch 14 Ethernet connection 12 Power cord 15 Accessory connection 13 Top cover screws 3 1 1 Functions and descriptions of main parts Pat Function description Touch screen Displays the control software and command buttons see Section 4 for detailed information Ex vivo button Starts the ex vivo timer Start button Starts the stabilization process Sample tray Holds the Maintainor card and moves the Maintainor card into a
12. denalor o TABILIZOWORK 141 Content 1 Introduction sasa a 5 1 1 TERMINOLOGY DEFINITIONS 7 2 Safety information 8 2 1 IMPORTANT USER INFORMATION 8 2 1 1 Intended use aaa 8 2 1 2 Warning caution and note definitions 8 2 1 3 Recycling sax awan KEAN e RE 8 2 2 LIST OF WARNINGS AND CAUTIONS 9 3 Stabilizor T1 instrument 10 3 1 MAIN PARTS 6 0 0 assis da awed oe i ee ee ww 10 3 1 1 Functions and descriptions of main parts 11 3 2 INSTRUMENT SITE REQUIREMENTS 13 3 3 INSTALLING THE INSTRUMENT 13 3 4 MOVING THE INSTRUMENT 0 0005 14 4 Software ccc ces 15 4 1 GENERAL FUNCTION 00000 c eee eee 15 4 2 INSTRUMENT MODES 0000 0 a 16 4 2 1 Running mode 2 0 00 cc eee eee 16 4 2 2 Service mode 4 17 4 2 3 Transport mode 18 4 3 STABILIZATION SETTINGS 19 4 3 1 Set sample name 19 4 3 2 Set card number LL 20 4 3 3 Setmethod 0 00 0 0 eee 21 4 4 EX VIVOTIME 0 cee ee 23 45 LOGFILES 220 LL 23 4 6 OPTIONS 0 00 eee eee 24 4 7 SOFTWARE UPGRADE 0 0000 ee ees 24 Stabilizor T1 DPM 0008 Edition AC denalor Maintainor card 1 00 25 Bil STORAGE sosia bod Ia at de 25 Stab
13. e 4 4 4 EX VIVO TIME In this context the ex vivo time is the time from the sacrifice of the experimental animal or the collection of a tissue sample until the sample is stabilized The ex vivo time is reset to zero by pressing the ex vivo button on the touch screen Sample name N Card number Card type Et Method 5 It is important to start the ex vivo time measurement just prior to the sacrifice of the animal or removal of the sample whatever occurs first 4 5 LOG FILES A log file including information on the samples are automatically saved to an external USB flash drive if inserted The instrument will alert you if the external USB flash drive is full If no external USB flash drive is inserted into the instrument the log file is saved temporarily on the internal USB flash drive until one is inserted A large number of stabilization runs can be saved on the internal USB flash drive To minimize the risk of accidental loss of log data due to any unexcpected failure always use an external USB flash drive inserted into the instrument The log file marked with the date e g 21May2008 is saved into a folder called Denator created by the software All runs during a day is saved into the same log file The log file includes the following data for each sample Time Sample name Maintainor ID s Ex vivo time Run time SET Temperature Actual Temperature Vacuum Sample height Method used and Comments Th
14. e log files are in the format Microsoft excel xls Stabilizor T1 DPM 0008 Edition AC 23 denalor 4 6 OPTIONS Figure 8 The Options screen Button Function description Service mode Use to clean the instrument See Section 4 2 2 and or change needle device see Section 7 3 or O ring see Section 7 2 Transport mode Use to move the instrument See Section 4 2 3 H W and S W Use to check software version and to update the software See revision Section 4 7 Screen calibration Use to align the screen Height calibration Height calibration must only be performed by authorized service personnel Please contact Denator Adjust clock Use to adjust the time and date Exit Use to exit the Options screen 4 7 SOFTWARE UPGRADE 24 Software upgrades will be distributed by Denator 1 Insert the USB flash drive with the upgraded software version Wait until the USB symbol appears on the touch screen EI Press the OPTIONS button Select H W AND S W REVISION aoa Km O N Press UPDATE Caution Do NOT turn off the instrument during the upgrade as this may cause irreversible malfunction Stabilizor T1 DPM 0008 Edition AC 5 Maintainor card Maintainor card 5 Maintainor card see Figure 9 is designed for use in Stabilizor T1 to quickly stop ex vivo degradation of proteins in fresh or frozen tissue samples up to 7 mm thick Rapid thermal inactivation stops all protein degradation processes simultaneously and st
15. ent has been turned off and allowed to cool down for 30 minutes gt BB 7 Remove the white O ring Stabilizor T1 DPM 0008 Edition AC 35 denalor 9 36 Put on a new O ring Make sure that the O ring is not twisted and that it is fitted securely in the groove a 10 If required clean the instrument see Section 7 4 Stabilizor T1 DPM 0008 Edition AC Maintenance 7 7 3 CHANGING THE NEEDLE DEVICE AN Warning Sharp needle Take care when handling the needle device to avoid injury A Needle guide B Needle holder C Needle device D Tube interlock E Vacuum connection 1 Make sure that there is NOT a Maintainor card in the sample tray 2 Set the instrument to Service mode by pressing OPTIONS and then SERVICE MODE see Section 4 2 2 3 Turn off the instrument Stabilizor T1 DPM 0008 Edition AC 37 denalor 4 Push the sample tray gently forward Caution It is only possible to move the sample tray if the instrument was set to Service mode before it was turned off 5 Remove the tube from the tube interlock and the vacuum connection 6 Carefully remove the needle device a Press the wings of the needle device together b Remove the needle device in the tube direction b 7 Dispose of the needle device in accordance with local regulations 38 Stabilizor T1 DPM 0008 Edition AC Maintenance 7 8 Fit the top of the needle into the hole of the needle guide and gently in
16. ilizing samples 26 6 1 PREPARATIONS cnc 26 6 1 1 Planning the experiment 26 6 1 2 Starting the instrument 27 6 1 3 Preparing the Maintainor card 28 6 1 4 Instument preparations 28 6 2 SAMPLE COLLECTION AND STABILIZATION 29 6 2 1 Sample collection 29 6 2 2 Sample stabilization 31 6 3 LOGFILES 0 ccc cee ee ees 32 Maintenance eee es 33 7 1 MAINTENANCE AND SERVICE 33 7 2 CHANGING THE O RING 002 aaa 33 7 3 CHANGING THE NEEDLE DEVICE 37 7 4 CLEANING THE INSTRUMENT 40 Technical specification 41 Stabilizor TT 41 Maintainor i 41 Ordering information 42 NOT e e e sit simi lenti bit inni 42 Stabilizor T1 DPM 0008 Edition AC Introduction 1 1 Introduction Comparative protein studies are dependent on the reliability of the sample to reflect as accurately as possible the in vivo state of the measured macromolecules Ex vivo degradation is a rapid process It causes significant changes in the levels of unstable macromolecules e g proteins see Figure 1 This causes problems with reproducibility and detectability only minutes after sample extraction and could even yield false positives It is therefore of utmost importance to minimize and standardize the time between
17. izor T1 DPM 0008 Edition AC 15 denalor 4 2 INSTRUMENT MODES There are 3 different modes in which the Stabilizor T1 can operate e Running mode e Service mode e Transport mode 4 2 1 Running mode In running mode the instrument is ready for stabilizing samples To set the instrument in Running mode If the instrument is not turned on 1 Turn on the instrument see Section 3 3 The instrument automatically enter Running mode displaying the main screen Sample name 5 Card number Card type l Method TURE 2 Wait until the startup sequence has finished and the heating blocks have reached the correct temperature normally 95 C Stabilization cannot be performed until the set temperature of the heating blocks has been reached as indicated by the text wait on the start button If the instrument is turned on and is in cleaning or transport mode Follow the instructions on the screen to return to running mode 16 Stabilizor T1 DPM 0008 Edition AC Software 4 422 Service mode In service mode the instrument is prepared for maintenance e g change of the O ring on the lower heating block The upper heating block is raised to an extra high level creating space for handling the O ring and the heating of the heating blocks is stopped The instrument will not move any parts when in service mode However it is possible to manually move the sample tray for easy access when cleaning see Section 7 4 It is
18. nd out of the heating block compartment Maintainor card Container for the sample during the heating process and after Stabilizor T1 DPM 0008 Edition AC 11 denalor Pat Function description Needle parts Heating compartment O ring Illumination USB port Laser Needle device Penetrates the vacuum seal to remove air from the Maintainor card e Needle holder and Needle guide Holds the needle device in the correct position Needle shuttle lever Moves the needle device into and out from the vacuum channel of the Maintainor card Chamber for the heating blocks with a controlled temperature environment Not shown in Figure 4 5 Contact vacuum seal placed on the lower heating block for optimal contact between the Maintainor card and the lower heating block Indicates instrument status by different colors e Blue The instrument is on and a Maintainor card can be placed in or removed from the sample tray e Green The Maintainor card has been successfully processed Red Malfunction of the instrument Restart the instrument 3 ports available Used for USB flash drive to collect and store the log file from the run Software updates are installed via a USB flash drive Capture the thickness of the sample to ensure reproducibility by adjusting the stabilization time to optimize sample stabilization Service connectors Used for service purposes 12 Stabilizor T1 DPM 0008 Edition
19. ode The information is saved in the log file see Section 4 5 4 3 1 Set sample name How to enter sample name 1 Press the orange sample name text to open the keypad I Sample name Card number oo Card type tissue Method 2 Enter the sample name and press OK to return to the main screen If required press the 123 selection button to be able to enter digits and symbols Stabilizor T1 DPM 0008 Edition AC 19 denalor 4 3 2 Set card number All Maintainor cards have room for customer IDs and or bar codes Once a sample is stabilized the card number used for the first sample is automatically increased with one to simplify the stabilization settings for next sample How to enter card number 1 Press the orange card number text to open the key pad Sample name Card number I Card type Method 2 Enter the card number and press OK to return to the main screen 20 Stabilizor T1 DPM 0008 Edition AC Software 4 4 3 3 Set method There are four different methods that can be used for stabilization and one method for vacuum treatment of sample Method Recommended use and description STRUCTURE To be used for fresh and frozen tissue that must maintain its PRESERVING structure i e that must not be squeezed The method includes automatic measurement of the sample and adjustment of the stabilization time to ensure that all parts of the tissue reach the correct stabilization temperature
20. power switch The instrument performs a self test and then stops in running mode ready to start a run Stabilizor T1 DPM 0008 Edition AC Software 4 4 Software 4 1 GENERAL FUNCTION Stabilizor T1 is controlled via a touch screen on the front of the instrument T i am ag Sample name 2 Mi i 7 Card number a ai Card type 3 Method STRU E 9 10 4 9 11 Figure 7 The touch screen of Stabilizor T1 main screen Lo Instrument part Kao Instrument part 2 O O SA W Current date and time Buttons with editable text Heating block temperatures 8 Ex vivo button with ex vivo time set upper and lower Selected method 9 Method selection button USB flash drive if inserted 10 Start button Error message symbol 11 Options button Ex vivo time To edit sample name or card number press the button to display a key pad view To select method press the method selection button to display a new view with selection buttons For detailed information see Section 4 3 The ex vivo button display the current ex vivo time Press the button to reset the ex vivo time Press the error message symbol to display the error message To the left there is current information time date heating block temperature and selected method All sample data is saved and can be viewed later see Section 4 5 Maintenance requests are automatically displayed on screen when needed For information on the options see Section 4 6 Stabil
21. sert the needle device into the needle holder gt Make sure that the needle is inside the hole of the needle guide 9 Press down the tube with one finger 1 and then fit the tube 2 into the tube interlock D gt Correctly fitted needle device to the left Otherwise re fit the tube into the tube interlock so that a downward pressure is applied to the needle device 11 Fit the tube into the vacuum connection E Stabilizor T1 DPM 0008 Edition AC 39 denalor 7 4 CLEANING THE INSTRUMENT If the instrument or area under the sample tray needs to be cleaned set the instrument in service mode see also Section 4 2 2 With the instrument on 1 With the instrument on press OPTIONS in the main screen Sample name NO NAMI Card number I Card type Method 5 TRUC 2 In next view press SERVICE MODE 3 Wipe the area under the sample tray with a tissue moistened with 70 EtOH 4 If required clean the outside of the instrument as well 5 Follow the instructions on screen to return to running mode 40 Stabilizor T1 DPM 0008 Edition AC With the instrument turned off Technical specification 8 1 Move the sample tray out of the way manually It is only possible to move the sample tray if the instrument was set to Service mode before it was turned off 2 Clean the area as in step 3 and 4 above 8 Technical specification Stabilizor T1 Dimensions Net weight Mains po
22. the needle device see Section 7 3 e Cleaning the instrument see Section 7 4 The Stabilizor T1 should always be put into service mode see Section 4 2 2 before performing any maintenance or cleaning 7 2 CHANGING THE O RING For optimal contact and thereby heat transfer between the lower heating block and the Maintainor card during stabilization the space between the two parts sealed by an O ring is evacuated during the stabilization 1 With the Stabilizor T1 instrument turned on press OPTIONS in the main screen Sam ble name Card number Card type Method Stabilizor T1 DPM 0008 Edition AC 33 denalor 34 In next view press SERVICE MODE The upper heating block will be lifted to an extra high level and the heating of the blocks is turned off Turn off the instrument using the Power switch as instructed on the screen and disconnect the mains cord Allow the heating block to cool this usually takes 30 minutes Remove the two screws on the back of the instrument using a Torx T20 tool Stabilizor T1 DPM 0008 Edition AC Maintenance 7 6 Lift off the top cover Warning Hazardous voltage Stabilizor T1 contains mains voltage of up to 240 V ac Disconnect the power cord before removing the top cover Warning Laser inside The instrument must always be turned off when the top cover is removed Warning Hot parts inside Do not remove the top cover unless the instrum
23. wer requirements Fuses Power consumption Ambient temperature Maximum relative humidity Touch screen dimensions Vacuum Noise Laser Embedded processor Maintainor Dimensions Sample chamber dimensions Maximum relative humidity Material Operating temperature Stabilizor T1 DPM 0008 Edition AC 465x306x143 mm LxWxH see also Figure 6 6 7 kg 100 240V 50 60Hz T4A H 250V 350W max 70W typical use 20W standby mode with heating elements off 10 to 40 C 80 non condensing 4 3 10 100 mbar underpressure applied to Maintainor card 45 AB A CLASS 1M BLUE laser 1 GHz x86 1 GB RAM Flash based solid state hard drive Linux base open source software 85x54x2 mm LxWxH Height 7mm Diameter 33 mm 85 non condensing PC and Teflon 80 to 100 C 41 42 denalor 9 Ordering information NOTES Stabilizor T1 Maintainor Tissue O ring Needle devices Pack size 1 DST 0001 12 pack DMT 0001 2 DST 0003 2 DST 0008 Stabilizor T1 DPM 0008 Edition AC denalor Stabilizor T1 User Manual DPM 0008 Edition AC March 2010 Denator AB First published September 2008 Stabilizor and Maintainor are trademarks of Denator AB All third party trademarks are the property of their respective owners Denator AB Contact Arvid Wallgrens Backe 20 SE 413 46 Gothenburg Sweden Phone Fax E mail Website Support E mail 46 0 31 412 841 46 0 31 412 840 inform denator com
24. without destroying the structure AUTO FRESH To be used for fresh tissue that does not need to keep its structure SAMPLE The method include automatic measurement of the sample and adjustment of the stabilization time to ensure that all parts of the tissue reach the correct stabilization temperature The sample is heated between both heating blocks to speed up the stabilization procedure AUTO FROZEN To be used for all tissue below 0 C down to 80 C that does not SAMPLE need to keep its structure The method includes automatic measurement of the sample and adjustment of the stabilization time to ensure that all parts of the tissue reach the correct stabilization temperature The sample is heated between both heating blocks to speed up the stabilization procedure CUSTOM Manual mode where all parameters can be set by the user VACUUM PACK To be used for setting a sample under vacuum without heating Useful when the sample needs to be stored frozen Stabilizor T1 DPM 0008 Edition AC 21 denalor How to select method 1 Press the orange method text to open the method selection dialog Sample name MO NAMI Card number I Card type nga Method TURI 2 Press the applicable method button to select the method and return to the main screen If you selected CUSTOM continue with setting the parameters in the new screen before pressing OK to return to the main screen 22 Stabilizor T1 DPM 0008 Edition AC Softwar
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