ElectroPrep System User`s Manual
Contents
1. Recessed power connectors integral with the safety lid 0 2mm diameter platinum electrodes 99 99 pure User replaceable platinum electrodes Silicone rubber dovetail seal provides leak free sealing and are easy to clean and or replace User friendly clamping system Wide range of accessories Environmental Conditions This apparatus is intended for indoor use only This apparatus can be operated safely at an altitude of 2 000m The normal operating temperature range is between 4 C and 65 C Maximum relative humidity 80 for temperatures up to 31 C decreasing lin early to 50 relative humidity at 40 C The apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664 POLLUTION DEGREE 2 states that Normally only non conductive pollu tion occurs Occasionally however a temporary conductivity caused by con densation must be expected Packing List Electroprep No Items Description 1 TANK LID AND CONNECTERS 1 SET OF POWER SUPPLY ADAPTERS 1 REPLACEMENT GASKET Harvard Apparatus ElectroPrep System Using the Electroprep Unit General Care and Maintenance Tank Disconnect leads from power supply before servicing unit To remove the safety lid push thumbs down on the plastic lugs and lift the lid vertically with your fingers Before use clean and dry the apparatus with DISTILLED WATER ONLY IMPORTANT Acrylic plastic is NOT resistant to aromatic or halogenated hydrocarbons ketones2. 74 1101 68 0609 FAST DIALYZER includes tank lid test leads and gasket 200Vdc 100mA Chamber Volume Pkg of 1 Pkg of 5 50ul 74 0408 74 0400 100ul 74 0409 74 0401 200ul 74 0410 74 0402 500ul 74 0411 74 0403 1000ul 74 0412 74 0404 1500ul 74 0413 74 0405 LINK CHAMBERS Chamber Volume Pkg of1 Pkg of 5 25ul 74 1619 74 1620 50ul 74 1611 74 1615 100ul 74 1612 74 1616 250ul 74 1613 74 1617 500ul 74 1614 74 1618 Pkg of 1 74 0100 74 1105 74 0102 doins Fast Dialyzers Any tvvo chambers vv volume range of 50ul 200ul Any tvvo chambers vv volume range of 250ul 1500ul Any chamber vv volume of 50ul 200ul to any 250ul 1500ul chamber Chamber Volume 2 Harvard Apparatus ElectroPrep System Ordering Information A large variety of membranes are avallable for use vvith our different dialysis prod ucts The follovving tables are designed to assist you in choosing the appropriate membranes for your needs Specialty membranes are also available Contact Harvard Apparatus for any custom membranes Cellulose Acetate These membranes are lovv protein binding and have a sharp MVVCO range The membranes are pre cut and supplied in 0 05 sodium azide solution They are ready to use after rinsing with deionized water and buffer Glycerol sulfur and heavy metals are not present in these membranes The cellulose acetate mem branes are intended only for aqueous solutions an
3. esters alcohol s over 25 and acids over 25 they will cause crazing especially of the UV transparent plastic and should NOT be used for cleaning DO NOT use abrasive creams or scourers Dry components with clean tissues prior to use Before use and then on a monthly basis check the unit for any leaks at the bonded joints Place the unit on a sheet of dry tissue and then fill with DIS TILLED WATER ONLY to the maximum fill line Any leakage will be seen on the tissue paper If any leakage is seen DO NOT ATTEMPT TO REPAIR OR USE THE APPARATUS but notify Harvard Bioscience immediately The replacement platinum electrodes are partially shrouded for protection However when cleaning the main tank DO NOT use cleaning brushes in the electrode area Usually a thorough rinse with distilled water is all that is required Ensure that the connectors are clean and dry before usage or storage Teflon Chambers e Clean by rinsing with DI water prior to each use e Take care to not damage the threads e For cases where contamination is severe low concentration detergents may be used followed by rinsing thoroughly with DI water Membranes e Prior to use rinse with DI water and treat with your buffer solution Membranes should be discarded after use do not reuse 2 Harvard Apparatus ElectroPrep System Operation Electroprep Protocols A 1 Electro Elution from Gel Pieces Choose a elution and sample c
4. ElectroPrep System User s Manual Publication 9511 040 HARVARD APPARATUS WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any changes as they apply Do Not Dispose Produc
5. d the presence of an organic solvent is not recommended Regenerated Cellulose These membranes are more stable in organic solvents but the MWCO range is not as sharply defined as that of cellulose acetate membranes The membranes are pre cut and supplied in a 0 05 sodium azide solution They are ready to use after rinsing with deionized water and buffer Glycerol sulfur or heavy metals are not present in these membranes Polycarbonate These membranes are more stable in organic solvents They are available in four highly controlled pore sizes for a well defined MWCO range Membranes for DIALYZER pack of 25 CHAMBER VOLUME CHAMBER VOLUME 5757 5 Daltons ACETATE CELLULOSE ACETATE CELLULOSE 7424 CA100 7425 CA100 7424 CA500 gt 7425 CA500 7424 1 7427 RC1K 7425 CA1K 7428 RC1K 7424 CA2K 7427 RC2K 7425 CA2K 7428 RC2K 7424 CA5K 7427 RC5K 7425 CA5K 7428 RC5K 7424 CA10K 7427 RC10K 7425 CA10K 7428 RC10K 7424 CA25K 7427 RC25K 7425 CA25K 7428 RC25K 7424 CA50K 7427 RC50K 7425 CA50K 7428 RC50K 7424 CA100K 7425 CA100K 7424 CA300K 7425 CA300K EE 1 O 7431 PC01 7432 PC01 7431 PC05 7432 PC05 7431 PC10 7432 PC10 7431 PC60 7432 PC60 Notes L 5 1 snyeseddy pieasey
6. enty four 24 months from the date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty extends to the equipment provided it has been used under normal laboratory conditions and in accordance with the operating limitations and maintenance procedures outlined in this instruction manual and when not having been subject to accident alteration misuse or abuse No liability is accepted for loss or damage arising from the incorrect use of this unit Harvard Biosciences lia bility is limited to the repair or replacement of the unit or refund of the purchase price at Harvard Bioscience s option Harvard Bioscience is not liable for any consequential damages If a defect arises within the two year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Massachusetts 01746 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless a RMA return materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other ri
7. ghts which vary from state to state REFER TO THE PACKING LIST AND CHECK THAT ALL COMPONENTS AND ACCESSORIES ARE PRESENT PLEASE RETAIN ALL PACKAGING MATERIALS UNTIL THE WARRANTY PERIOD HAS EXPIRED Harvard Apparatus products are for research use only and not for clinical use on human or veterinary patients CAUTION FOR RESEARCH USE ONLY NOT FOR CLINICAL USE ON HUMAN OR VETERINARY PATIENTS 7 Harvard Apparatus ElectroPrep System General Safety Summary WARNING THESE UNITS ARE CAPABLE OF DELIVERING POTENTIALLY LETHAL VOLTAGE WHEN CONNECTED TO A POWER SUPPLY AND ARE TO BE OPERATED ONLY BY QUALIFIED TECHNICALLY TRAINED PERSONNEL PLEASE READ THE ENTIRE OPERATOR S MANUAL THOROUGHLY BEFORE OPERATING THIS UNIT THESE UNITS COMPLY WITH THE STATUTORY CE SAFETY DIRECTIVES 73 23 EEC LOW VOLTAGE DIRECTIVE IEC 1010 1 1990 plus AMENDMENT 1 1992 EN 61010 1 1993 BS EN 61010 1 1993 Please read the following safety precautions to ensure proper use of your ElectroPrep System To avoid potential hazards and product damage use this product only as instructed in this manual To Prevent Hazard or Injury Make Proper Connections Make sure all connections are made properly and securely Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qualif
8. hambers FastDialyzer union and membranes Note Various systems may be created with link chambers or unions added to FastDialyzer chambers See illustrations on following pages 2 3 7 Remove all caps from the chambers Place Membrane 1 lower molecular weight cutoff than your biomolecule on the membrane platform of the sample chamber and assemble a cap hand tighten Place Membrane 2 lower MWCO than your molecule may be equal to Membrane 1 into the elution chamber and assemble cap Assemble union onto other end Fill the ElectroPrep tank with your electro elution buffer Place the chambers into the tank Chambers and caps must be completely immersed in buffer so that no air bubbles are present 6 Add your gel slices to the elution chamber Place Membrane 3 MWCO larger than your molecule over the sample collection chamber and assemble the sample chamber to the union hand tighten Keeping all parts submersed in buffer prevents the introduction of air bubbles 8 Gently push the assemblage of chambers through the septum to secure the system in place Close the lid and use the current and voltage as required for the electrophoresis 15mA recommended Elution time can be calculated by measuring the time required for the molecule to migrate 1 cm during gel electrophoresis 2 Harvard Apparatus ElectroPrep System Operation Electroprep Protocols A Final Assembly f Fast Dialyzer 1 Fast D
9. ialyzer 2 Membrane 1 Membrane 3 Membrane 2 Separating plate for buffer solution s Union and Fast Dialyzer 1 are the elution chamber Fast Dialyzer 2 is the sample collection chamber m Membrane 1 amp 3 have MWCO smaller than molecule Membranes 2 have MWCO larger than molecule Applications Elution of DNA proteins or other biomolecules from a gel piece s Harvard Apparatus ElectroPrep System Operation Electroprep Protocols B Selective Electro Filtration Concentration Separation Based on Different Charges on Biomolecules Select the proper size dialyzer chambers and union to use as much of the available chamber volume as possible minimizes air gaps 2 Membranes are assembled by placing onto platform of FastDialyzer and hand tightening mating piece cap link or union a Union acts as the sample compartment Membrane 1 amp 3 have MWCO smaller than molecule m Membranes 2 have MVVCO larger than molecule Applications m Separation and purification of biomolecules vvith unknovvn isoelectric potential Sample Icompartment si Fast Dialyzer 1 l 2 Harvard Apparatus ElectroPrep System Operation Electroprep Protocols C Electrodialysis Through Simultaneous Exchange of Buffers Select the proper size dialyzer chamber use as much of the available chamber volume as possible minimize air gaps Membranes are assembled by placing ont
10. ied service personnel to perform inspection Place Product in Proper Environment Review the operating manual for guidelines for proper operating environments Observe all Warning Labels on Product Read all labels on product to ensure proper usage 7 Harvard Apparatus ElectroPrep System General Safety Summary Safety Precautions READ the instructions before using the apparatus Always isolate electrophoresis units from their power supply before removing the safety cover Isolate the power supply from the mains FIRST then disconnect the leads DO NOT exceed the maximum operating voltage or current DO NOT operate the electrophoresis units in metal trays Following the replacement of a platinum electrode have the unit inspected and approved by your safety officer prior to use DO NOT fill the unit with running buffer above the maximum fill lines DO NOT move the unit when it is running CAUTION During electrophoresis very low quantities of various gases are produced at the electrodes The type of gas produced depends on the com position of the buffer employed To disperse these gases make sure that the apparatus is run in a well ventilated area 7 Harvard Apparatus ElectroPrep System Specifications Construction Rugged acrylic construction All acrylic joints chemically bonded Doubly insulated cables rated safe up to 1 000 volts Gold plated electrical connectors corrosion free and rated safe up to 1 000 volts
11. o platform of FastDialyzer and hand tightening mating piece cap or link Membranes 1 amp 2 have a MWCO smaller than the biomolecule Applications 100 Primer removal after PCR in 5 10 minutes De salting of neutral materials that do not move in an electric fields Fast Dialyzer 2 Harvard Apparatus ElectroPrep System Operation Electroprep Protocols 0 Rapid and Selective Electro Filtration Concentration Select the proper size dialyzer chambers and union to use as much of the available chamber volume as possible minimize air gaps Membranes are assembled by placing onto platform of Fast Dialyzer and hand tightening mating piece cap link or union Sample compartment is comprised of the Union and Fast Dialyzer 1 Membranes 1 amp 3 have a MWCO smaller than the biomolecule Membrane 2 should have a MWCO larger than your biomolecule Sample is collected in Teflon Chamber 2 Applications Concentration of small samples for selective filtration m Fast Dialyzer 1 E Multi Chamber Systems Connection of numerous link chambers vvith membranes of different MVVCO can be used for highiy selective electro filtration and separation Link Chambers can also be used to increase the chamber volume of any Fast Dialyzer MEMBRANE LINK CHAMBER 2 M a FAST DIALYZER 2 Harvard Apparatus ElectroPrep System Ordering Information ElectroPrepa Tank Povver Supply
12. t with Municipal Waste Special Collection Disposal Required 3 Harvard Apparatus ElectroPrep System Table of Contents SUBJECT PAGE NO General Information Warranty and Repairs 2 General Saftey Summary 3 4 Specifications EE ONS LLG NON sallama ay s 5 Environmental Conditions 5 Packing Esin a selenin ikeli lee iel 5 Care amp Maintainence 6 Operation A Electro Elution from Gel 7 8 S 7 Final Assembly zad 8 B Selective Electro Filtration by Charge 9 C Electro Dialysis 10 D Rapid amp Selective Electro Filtration 11 Multi Chamber Systems 11 Ordering Information 12 13 Harvard Apparatus ElectroPrep System General Information WARRANTY THE ELECTROPREP UNIT IS DESIGNED TO GIVE LONG SERVICE AND REPRODUCIBLE RESULTS IN YOUR LABORATORY A FEW MOMENTS SPENT READING THESE INSTRUCTIONS WILL ENSURE THAT YOUR EXPECTATIONS ARE REFLECTED IN THE SUCCESSFUL USE OF THE APPARATUS FIRST CHECK THAT THE APPARATUS HAS BEEN RECEIVED COMPLETE AND UNDAMAGED FOLLOWING SHIPMENT ANY FAULTS OR LOSSES MUST BE NOTIFIED TO HARVARD BIOSCIENCE IMMEDIATELY Harvard Apparatus warranties this instrument for a period of tw
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