Biological Microscope User Manual
Contents
1. position then screw down the bolt to fix it TE Installing the eyepiece Fig3 4 Insert the eyepiece into the eyepiece tube until they are against each other as shown Fig 3 in Fig 4 BM 300 2 2 3 Installing the objective Fig 5 6 1 Adjusting the coarse focus knob until the support device of the mechanical stage reaches its low limit position 2 Screw the lowest magnification objective Fiss into the nosepiece from the left or the right side then revolve the nosepiece clockwise and mount other objectives by the sequence Sc of low to high magnification DN gt lt V Installing objective this way will make the WS change of magnification to be easier during using Clean the objective regularly for lens is susceptible to dust When operating use 10xmagnification objective to search and focus specimen firstly then replace with higher magnification objective if necessary When replacing the objective slowly turn the nosepiece until you hear clicked that means the objective is in place 2 4 Mounting the filters Fig 7 Fig 7 1 Place the required filter in front of the condenser the filter of the standard outfit is green and baby blue Fig 8 Fig 10 2 2 5 Connecting the Power Fig 8 10 The2. Fig 20 Fig 21 The interpupillary distance range 55mm 75mm While looking through the eyepieces move both eyepieces until the left and right fields of view coincide completely 3 3 7 Adjusting the Diopter Fig 22 Turn the eyepiece to adjust the diopter while looking through it The diopter range of the eyepiece is 5 diopter The number aligned to the line on the viewing head is the diopter in use 3 3 8 Adjusting the tension adjustment collar Fig 23 Turn the tension adjustment collar with your fingers When the collar is turned in the direction of the arrow tension of the coarse adjustment knob increases Turning the collar in the opposite direction decreases the tension If the nosepiece descends on its own or if the specimen gets out of focus quickly even when it is brought into focus using the fine adjustment knob 3 it means the tension of the coarse adjustment knob is too low Turn the collar in the direction of the arrow to increase the tension 4 Technical Specifications 1 Main Specifications BM 300 Optical System Infinite optical system oo Compensation Free Binocular or Trinocular Head 30 Inclined Viewing Head Interpupillary Distance 48 75 mm Eyepiece Wide Field Eyepiece with field of view 18 mm Nosepiece Quadruple Nosepiece Infinitive Semi plan Objectives 4x 10x 40x 100x Objective Focusing Coaxial Coarse and Fine Ad
3. 6 Troubleshooting Guide BM 300 1 Optical System TROUBLE CAUSE SOLUTION The nosepiece is not in the located position Locate the nosepiece properly 1 The edge of the field of objective and light path not coaxial where it clicks view is dark or the brightness The surface of the lamp becomes black Change a new lamp bulb is not uniform A lens the objective condenser eyepiece or f J gt gt amp YEP Clean it thoroughly collector is dirty Ditor dastis visible in Dirt dust on the specimen Replace with a clean specimen Add cover glass on it The thickness of the cover glass is not Use standard cover glass with suitable thickness of 0 17mm Specimen is placed reversely Dry pupe has oil on it especially for Wetea 40X objectives 3 Visibility 1 poor A lens the objective condenser eyepiece or f Image is not sharp es ai Clean it collector is dirty Contrast is poor Details are indistinct Immersion oil is not used with the 100x Ubeida objective Air bubbles existed in the immersion oil Eliminate the bubble The aperture iris diaphragm is stopped down Adjust the aperture iris too far diaphragm properly f Center the condenser with the Condenser is not properly centered centering screw 4 One side of image is The nosepiece is not properly engaged Engage the nosepiece properly blurred en The specimen is not clamped Clamp it with stage clips 5 The image is yellowish The blue filter is not
4. cord and set the main switch to state ON 2 Turning the brightness adjustment knob clockwise the voltage raise and the brightness strengthen whereas turning at the contra direction the voltage decline and the brightness weaken Fig 13 k Using the microscope with low voltage in the voltage range can prolong the service life of the bulb 3 3 2 Placing the Specimen Fig 17 1 Place the specimen in the center of the mechanical stage and use the stage clips to clamp it 2 Turn the portrait and lateral adjustment knob of the mechanical ruler move the specimen to the required position Be careful when changing the objective If you Fig 14 finish the observation with the short working distance objective and want to change another one be careful of not letting the objective touch the specimen 3 3 3 Focusing the Specimen Fig 18 1 Focus the specimen with 10X objective To avoid the objective touching the specimen during focusing you 1 2 3 should raise the mechanical stage to let the specimen close to the objective at first then slowly separate them to bring the specimen to focus Fig 15 Fig 16 gt Fig 17 Fig 18 BM 300 Turn the coarse focus knob conversely to lower t
5. used Use the blue filter The aperture iris diaphragm is too small Adjust it properly 6 The brightness is not The condenser is too low Adjust it properly enough A lens the objective condenser eyepiece or Clean it collector is dirty BM 300 2 Mechanical System TROUBLE CAUSE SOLUTION LI t ae nn Be When adjusting stage height you forgot Reattach upper stopper screw to reattach upper stopper screw PP PP focused 2 Objective makes contact with specimen Specimen is mounted upside down Mount specimen correctly before focus is obtained 3 the specimen can not be moved freely The slide is not clamped Clamp the slide firmly 4 Field of view of one eye does not match that of the other Interpupillary distance is incorrect Adjust interpupillary distance The diopter is not proper Adjust the diopter properly 5 Observation is tiring The brightness of the illumination is not proper toreves Adjust the lamp voltage 2 Electrical System TROUBLE CAUSE SOLUTION The bulb cannot est No power supply Check the power cord connection 1g The bulb burns out The voltage is too high Lower the voltage suddenly the illumination is not j The voltage is too low Raise the voltage bright enough The bulb is about to burn out Replace with a new one Image flicks The bulb is not inserted deeply Check its connection
6. LA BOECO P Germany Biological Microscope BM 300 User Manual This manual is for biological microscope Model BM 300 To ensure the safety obtain optimum performance and to familiarize yourself fully with the use of this microscope it is recommended strongly that you study this manual thoroughly before operating the microscope Boeckel Co GmbH Co KG CONTENTS BM 300 USER NOTICES cesses son son ons nn cesses cesses san sen sn ns sen nn ee 2 1 Components Name c srr see ses see cee eee cee cee cee cee nennen sense 3 2 Assembly een cee cee nenn cee cee cee eee coe en 4 2 1 Assembly Diagram erkennen nennen cee cee nen nennen nennen 4 2 2 Assembly Procedures een cse cee een 5 3 Adjustment cco coo coo ooo 000 0o00 000000 000000 000000 000000 000000 000000 000000 000 8 31 Adjustment Set Diaphragm cececocooooocoocoooooooocoocoooooooooooooooooooo 8 3 2 Adjustment Procedures res ete tte t tect eee nennen nennen nenne 11 4 Technical Specifications cco coo 000 ooo ooo Coe Coe 0o00 000 000000 000000 000000 000 13 6 Trouble Shooting cco coo ooo ooo ooo ooo ooo ooo ooo 000 0o00 000 000000 000 000 000 000 15 USER NOTICE BM 3BB 1 Safety Note 1 Open the box carefully to avoid the accessories like lens dropping to ground or being damaged 2 Do keep the instrument out of direct sunlight high temperature or humidity dusty and easy shaking environment Make sure the stage is flat horizontal and firm enoug
7. cable and cords are vulnerable when bent or twisted never subject the power cord to excessive force 1 Set the main switch to O off state before connecting the power cord 2 Insert the plugs into the power jack of the microscope safely 3 Plug the power cord into the power supply receptacle Make sure the connection is well Do use the supplied power cord all the time If lost or damaged select the same standard cord please A wide range of voltage like 100V 240V is acceptable for this microscope 2 2 6 Replacing the fuse Fig 8 10 Do remember to set the main switch to the state O OFF and unplug the power cord before replacing the fuse Rotate the fuse out of the holder with the type screwdriver replace with a new fuse then rotate it back to the holder again 3 1 Adjustment set diagram Fig 14 15 Woes Lateral Adjustment Knob Right Fine Focus Knob Brightness Adjustment Knob Right Coarse Focus Knob Tension Adjustment Collar Fig 11 Condenser Focusing Knob Interpupillar Distance Indicator O Clocking Set Indicator Left Fine Focus Knob Left Coarse Focus Knob Fig 12 Aperture Iris Diaphragm Collar BM 300 3 2 Adjustment and Operation 3 2 1 Brightness Adjustment Fig 16 1 Connect the power
8. h 3 When moving the microscope carefully carry it with the handle and the base 4 When running the lamp house and nearby parts will be very hot Please ensure there is enough cooling room for them 5 Make sure the instrument is earthed to avoid lighting strike 6 For safety be sure the main switch is in O off state and cut off the power supply before replacing the bulb or the fuse If you replace the bulb during use or right after use allow the lamp bulb and the lamp house to cool completely before touching Designated bulb 3W LED which is used with the electricity source since it cannot be charged 7 Check the input voltage be sure the input voltage that is signed in the back of the microscope is consistent with the power supply voltage or it will bring a serious damage to the instrument If they are different with each other you can change the input voltage by switching the voltage changeover switch 8 Always use the power cord provided by Novel i Maintenance and Care All the lenses have been adjusted properly do not dismount them by yourself please 2 The nosepiece and coarse and fine focusing parts are so delicate that it is forbidden to disassemble them carelessly by yourself 3 Keep the instrument clean and do not pollute the optical element when wiping away the dust on the instrument 4 The contaminations on the prism like fingerprints and oil smudges could be gently wiped with a piece of soft c
9. he specimen and search images in the 10xocular simultaneously and then use the fine knob to focus After that you can replace with other magnification objectives safely and focus without the risk of damaging the specimen To make the observation more convenient you can use the locking set to fix the stage in a vertical direction 3 3 4 Condenser Adjustment Fig 20 Turn the condenser focus knob to move the condenser up and down Raise the condenser when using the high magnification objective and descend it when using the low magnification one The condenser and the objective are coaxial It has been adjusted before leaving factory so the user needn t to adjust them by self The highest position of the condenser has been adjusted too It also needn t any user s operation 3 3 5 Aperture Iris Diaphragm Adjustment Fig 20 Turn the aperture iris diaphragm collar to adjust the aperture iris diaphragm The aperture iris diaphragm is designed for the adjustment of the numerical aperture not for the brightness Generally setting the aperture iris diaphragm to 70 80 of the N A of the objective in use will provide an image with good contrast If you want to observe the image of the aperture iris diaphragm remove one eyepiece and look through the tube You will see a dark circle encroaching on the bottom of the tube 3 3 6 Adjusting the Interpupillary Distance li
10. justment Moving Range20mm Fine Division 0 002mm Abbe Condenser NA 1 25 Condenser St Double Layers Mechanical Stage 140mmx140mm Moving Range 75x50mm age Illumination 3W LED 2 Eyepiece and objectives 1 Infinitive Semi Plan Objectives Magnification Numerical Aperture Poem Seen eee Objective 4x 0 10 45 3 17 dry 10x 0 25 17 96 6 dry 40x 0 65 4 5 0 37 dry 100x 1 25 1 81 0 13 oil 2 Optional Eyepiece Eyepiece Magnification Focal Length mm Field of View mm Wide field plan eyepiece 25 020 5 Outfit BM 300 Components Name Specification Quantity Outfit f O Main Standard 1 O Main Body Double Layers Mechanical Stage 1 O Condenser Holder 1 O Compensation Free Binocular Head 1 Viewing Head Compensation Free Trinocular Head 1 O Condenser Abbe Condenser NA 1 25 1 O Nosepiece Quadruple 1 O 3W LED 1 Illumination O 2 Spare BGX 1 20 0 25A Fuse 2 En O Eyepiece 10 Wide Field Plan Eyepiece 18 mm 2 10x Wide Field Plan Eyepiece 920 mm 2 i DODN O Infinite Semi Plan Objective 4x 1 fd O Infinite Semi Plan Objective 10x 1 Objective O Infinite Semi Plan Objective 40x 1 Infinite Semi Plan Objective 100x Oil i O Bright Field Condenser with Adjustable O Condenser BEER 1 Iris Diaphragm Filter Baby Blue Green 1 for each O o Standard Outfit e Optional
11. loth or tissue paper gauze which has been immersed in pure alcohol or ether Note that the alcohol and ether are highly flammable do keep them away from the fire or potential sources of electrical sparks and use them in a drafty room as possible as you can 5 Do not attempt to use organic solvents to clean the microscope components other than the glass components To clean them use a lint free soft cloth slightly moistened with a diluted neutral detergent 6 When using if the microscope is splashed by liquid cut off the power at once and wipe away the splash 7 Do not disassemble any parts of the microscope as this will affect the function or reduce the performance of the microscope 8 Place the instrument in a cool dry position When not using the microscope keep it covered with a dust cover Make sure the lamp socket is cool before covering the microscope 2 Assembly 2 1 Assembly Diagram The following figure shows the installation sequence of the components The number in the figure shows the assembly steps Before installing be sure every components is clean do not score any parts or glass surface Keep well with hexagon wrench provided When changing the components you will need it again Binocular 10X Eyepiece viewing head 2 2 Assembly Procedure 2 2 1 Installing binocular viewing head Fig 1 2 Insert the binocular viewing head into the microscope head and turn it to a proper
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