Elisys Uno
Contents
1. Start of Day This will completely prime the sample handling system with the fresh deionized water It also primes the washer if applicable with wash solution and turns on the lamps Observe the fluid handling system and ensure there are no leaks nen Routines Lar Regata un lt X Backing Pattie s Tg amp amp FE 200 OOo oi eimi mis artio OOOOOOOi Figure 2 3 1 Start of Day After running the Start of Day program visually check the sample handling tubing and the syringes for the presence of any bubbles If bubbles are present you should run the Start of Day program again tapping the tubing where the bubbles are present If this does not eliminate the bubbles run the Weekly Alcohol Cleaning see TroubleShooting Guide for details 2 4 Channel Blanks Select Channel Blank from the Settings gt Alignment menu dialog This will allow you to blank the four photometer channels on a wetted optically clear solution example blanking solution included 0 5N NaOH with 100uL Triton X 100 L The ELISYS UNO software will show you which wells it will be using for channel blanks preferably use a new set of wells and then prompt you to put ELISYS UNO Blanking Solution in position 1 of Rack 1 never use plain water Reset Channel Blank xj Please load wetted material to Rack1 1 and make sure wells 407 001 404 and DOM are all empty Click Start button to run Rack Plate JOGO OOOO njuna 00000 00000 02. in the accessories carton to the wash arm using the two attached thumbscrews The luer fittings must face out toward the user and the colour coded fittings on the tubing should be fastened finger tight to the wash head Refer to ee ia Figure 1 2 1 Wash Head Installation Before running the ELISYS UNO for the first time run the Performance Check as described in the User Manual Chapter 4 4 8 1 NOTE All of the steps in Section 2 Instrument Set Up must be complete before performing the Performance Check Human I Diagnostics Worldwide 5 12 Human ELISYS UNO Quick Start Guide 2 Instrument SetUp Before running any tests the instrument must be aligned and set up Use the procedures in this section to make sure your ELISYS UNO is ready to work When the software is first opened you are asked if the ELISYS UNO has a washer If no select No and again at confirmation 2 1 Alignment 1 Under the Settings menu go to Alignment and select Probe vs Rack1 E Settings Security Help Alignment Probe vs Rack Startup Probe ws Racks Database Probe vs Plate Report Probe vs Wash Cup Plate Wash Chemistry Mode Washer vs Plate Strategy Rack2 Dispense Height Channel Blank Display Parameters HGFEODCEA Figure 2 1 1 Settings gt Alignment Menu Al button to check the current alignment 2 Click the Probe vs Rack E4 elel g
3. then to an approved power source You are strongly advised to use an Uninterruptible Power Supply UPS to prevent the power from being interrupted to the ELISYS UNO and the computer 3 Place the Reagent rack on the left and the Sample rack on the right Next load the incubator plate with microwells and place it inside the plate carrier Reference Wash Station Photometer Wash Rinse and Waste bottlas Rack 1 Rack 2 Reaction Plate Reagent Rack Sample Rack Figure 1 1 4 Illustration 4 12 1 2 Instrument Check Out NOTE Put fresh deionised water in the Prime bottle before turning on the instrument With the instrument connected to your computer start the ELISYS UNO software then switch on the instrument When the instrument powers up note the following actions All racks move to the front home positions The probe moves to its home position to the left over the plate and then to the wash cup The syringe pumps prime The instrument and the optical system lights come on These events are controlled by firmware installed in your ELISYS UNO However the ELISYS UNO software must be running for proper operation If the ELISYS UNO power comes on but these actions do not occur and the ELISYS UNO continues to beep there is a problem with the communications setup Check the serial cable connections and COM port settings At this time the wash arm is raised from its shipping position Install the wash head found
4. 0000 000000000000 m OOOOOODODODDA 000000000005 000000000000 0000000000009 Result a ie Figure 2 4 1 Load the wetted Blanking material into position 10 12 The ELISYS UNO will then pipette the four blank wells read and store them then do a confirmatory reading The absorbance values of the confirmatory reading will be displayed and should be 0 0 0050A Reference Figure 2 4 2 Reset Channel Blank Results notice at the bottom of the window the result is shown Reset Channel Blank Please load wetted material to Rack1 1 and make sure wells A01 001 404 and D04 are all empty Click Start button to run Rack Plate Hi F EEDLC EEA BOE 00000000 666666660 MEDD 899900 000000000 DUO 2333328 HH A PTT os 2222132233223 11 O ODODODODO 12 Result FA5SSED channel blank has been reset with correct values 401 0 0006 D01 0 001 2 404 0 0006 D04 0 0014 Figure 2 4 2 Reset Channel Blank Results If one of the values is not between 0 0 0050 follow the instructions and try doing channel blanks again Make sure there are clean wells in the positions indicated to run channel blanks again If you continue to obtain incorrect channel blanks notify your instrument service provider 2 5 Display ELISYS UNO Parameters Choose Display ELISYS UNO Parameters in the Settings menu Notepad opens and the following text is shown Fe Ed Format Wesw Help Hri zod5 10 13 54
5. AM Idle 15 sending plate home IFERM Parsserers Block 1 D ari H 1040 1 ASK 1275 moja Z erg Kg 202 Kee MG Ne y ELE y ZmOLIO i me raeraTeters Black Mes Zeil Pras Zei ili Fu FAT wi Map ode san Poni Figure 2 5 1 Notepad file displayed by selecting Display ELISYS UNOParameters This file can be saved and or printed or filed for future reference Human I JJ u Diagnostics Worldwide 11 12 Human ELISYS UNO Quick Start Guide 12 12 HUMAN Gesellschaft fur Biochemica und Diagnostica mbH Max Planck Ring 21 65205 Wiesbaden Germany Tel 49 61 22 99 88 0 Fax 49 61 22 99 88 100 e Mail human human de www human de
6. ELISYS UNO Quick Start Guide Elis CE Cat No 17350 52 REVISION LIST OF THE MANUAL No Rev DATE REVISION DESCRIPTION 1 01 2006 08 First edition 2 02 2010 03 Adaptation to new Corporate Design Human gt Diagnostics Worldwide CONTENTS Getting Started 1 1 Software Installation 1 2 Instrument Check Out Instrument SetUp 2 1 Alignment 2 2 Rack 2 Dispense Height 2 3 Run Start of Day 2 4 Channel Blanks 2 5 Display ELISYS UNO Parameters O WD O UUU W 10 10 11 2 12 1 Getting Started 1 1 Software Installation 1 Turn your computer on and insert the ELISYS UNO installation CD The installer should auto start If not select Run from the Windows Start menu run CD ROM drive setup and follow the prompts for installing the program Run the ELISYS UNO program after installation Enter Admin as the user ID and password Review Figures 1 1 1 thru 1 1 3 for step by step software installation instructions Name Size _INST321 E _ 294 KB C _1SDEL EXE SKB Z setup dl 11 KB u osvsl cah u _userl cab a datal cab DATA TAG lang dat layout bin os dat A setup bmp SETUP EXE 59 KB SETUP INI 1 KB B setup ins 56 Kb setup lid 1 KB Ribe Execute SETUP EXE by double clicking Close all e programs click Next Application Application Extensi TAG File DAT File BIN File DAT File BMP File Application Conf
7. iguration Settings Internet Communic LID File Figure 1 1 1 Double Click to Begin Set Up Choose Destination Folder choose alternate folder if applicable Select Program Folder and click Next 2 Click Finish Setup has finished installing ChemWell 6 0 on your computer Setup Complete the options you wark below Click Finish to complete Setup Setup can launch he Read Me file and Chemwell 6 0 Choose bel Finishing Installation screen display c chemwell r6 assayschemperformance check Il em Figure 1 1 2 Click Finish Finish Button Figure 1 1 3 Finishing Installation Screen Display The ELISYS UNO Manager Icon and the Assay Editor Icon appear on Desktop By default the program uses COM1 for communication with the instrument If you have connected the instrument to a different port go to the Settings selectingthe communications port 3 12 Human ELISYS UNO Quick Start Guide menu and select ELISYS UNO after the software is installed Click OK after Human gt ms Diagnostics Worldwide sellings Communication Part COM GF Hahns Speed z Default Temperature Default Mode The first time ELISYS UNO Manager is opened you will need to use the Admin user name and password Once logged in as Admin you can create other user ID s and passwords as well as change your own password 2 Connect the power cord to the instrument
8. ing the bottom Use the arrows to move the probe into the correct position Press Test to confirm When finished click Save and then the Close button Probe vs Plate xj Figure 2 1 5 Adjust Probe Position for Plate 5 Select Probe vs Wash Cup from the menu Press the st button The probe will move to the center wash position and lower into the cup The probe tip should be centered in and at the surface of the small center wash cup If it is not in position use the arrow keys to move the probe into position When finished click Test to check new alignment If aligned click Save then Close Probe vs Wash Cup Im zi a Please wai Figure 2 1 6 Adjust Probe Position with Wash Cup 8 12 6 If your ELISYS UNO has a washer choose Washer vs Plate from the menu Insert a plate or strip tray when prompted Click the button The plate is moved under the wash head and the wash head is lowered into the wells The plate and wash head should be positioned so that the aspirate needles are centered left to right toward the back of the wells and touching but not pressing on the bottoms of the wells Use the arrows to position the Wash Head correctly When finished click Test to check new position If correct click Save then Close asher vs Plate q x Please wait Cave Close Figure 2 1 7 Align Washer and Plate 2 2 Rack 2 Dispense Height Under the alignment me
9. nu select Rack 2 Dispense Height to set the dispense height used when making predilutions in the Sample rack reference Figure 2 1 2 Adjust Probe Dispense Height Use the top set of three buttons to select the High dispense height and the bottom set of three buttons to select the Low dispense height the up and down arrows will adjust the probe up and down the a button will move the probe to its actual position setting Low is normally used for dispensing small volumes such as serum to a predilution High is normally used for dispensing a large volume of diluent these settings are specified when programming the assay in Assay Editor Low should be set inside the tube to eliminate splashing and loss of sample High should be set as high as possible to thoroughly mix the sample diluent but not high enough to cause reagent foaming Test with your particular reagents for appropriate dispense heights Click Save on each to save the new settings then click Close Rack Dispense Height 1 x Dispense High Bi gt Dispense Low Z sj Z s Please wait Close Figure 2 2 1 Adjust Probe Dispense Height Human I JJ u Diagnostics Worldwide 9 12 Human ELISYS UNO Quick Start Guide 2 3 Run Start of Day Check instrument fluid levels Fill the Prime bottle with fresh deionized water From the Routines menu select Start of Day refer to Figure 2 3 1
10. t l Figure 2 1 2 Adjust Probe Position for Rack1 The probe tip should be centered about 3mm above the rear pin as shown in Figure 2 1 2 Adjust Probe Position for Rack1 Use the green up and down blue side to side and red forward and backward arrow buttons to position the probe correctly Click Test to check new alignment When finished click Save then click Close 6 12 3 Next run Probe vs Rack 2 Align as shown in Step 2 for Rack1 Click Save then click Next Probe vs Rack Step 1 of 2 Figure 2 1 3 Adjust Probe Position for Rack2 The next step prompts you to replace Rack 2 and place your sample cup in position number 96 in the back of the sample rack Note If you do not want to set an alternate depth click Close Click the st button Use the arrow keys to move the probe until it almost reaches the bottom of the sample cup When the correct depth has been reached click Save and then the Close button Probe vs Rack Step 2 of 2 Now load Rack back and put a sample cup in position 96 Use Buttons to adjust the maximum probe depth Figure 2 1 4 Adjust Probe Depth for Rack2 Human ss III VJ i Diagnostics Worldwide 7 12 Human ELISYS UNO Ouick Start Guide 4 Select Probe vs Plate from the menu Insert a plate or strip tray into position when prompted Click the st button The probe tip should be centered in well HO1 and almost touch
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