Q-Grad Quaternary Gradient Pump
Contents
1. tubing may be cut with a plastic tubing cutter or razor knife 2 5 6 Priming the Pump and the Flushing Lines Be sure all of the connections downstream of the prime purge valve are closed Close all inlet shut off valves except for the pump head being primed Connect a syringe to the prime purge valve Open the prime purge valve 1 to 2 turns counter clockwise Run each pump head one at a time at a flowrate of 3 to 5 mL min Prime each pump head separately by pulling mobile phase and any air bubbles through the system and into the syringe a minimum of 20 mL Close the prime purge valve and stop the pump If fewer than four heads are being utilized prime the unused heads with solvent then seal the inlet valves with the supplied shipping plugs This will eliminate trapped air from the system that could corrupt gradient accuracy To prime the flush lines for a self flush head connect one of the small Luer to barb fittings to a syringe and pull 10 20 mL of flush solution through the outlet line at the top of the pump head 2 5 7 Pressure Fault System Protection The Q Grad monitors system pressure through Pump A and automatically turns the pump off if the pressure exceeds the maximum pressure limit This limit is determined by the head type or by user programmable upper and lower pressure limits The keypad controller or the PC driven software monitors the pressure transducer through Pump A For this reason Pump A must always be ut2. Figure 3 2 O Grad Pump Rear Panel Symbols The following symbols may appear on back panel of the pump Caution To avoid chemical or electrical hazards always observe safe laboratory practices while operating this equipment Caution To avoid electrical shock and possible injury remove the power cord from the back panel of this equipment before performing any type of service procedures Note The user shall be made aware that if equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired 3 2 1 Hardware Implementation Each REMOTE INPUT serial communications port is configured for 9600 baud 8 data bits 1 stop bit and no parity The connector is a standard RJ 11 modular telephone type jack The pin out is Function Ground DSR Handshaking input to pump RXD Serial data input to pump TXD Serial data output from pump DTR Handshaking output from pump 3 2 Special wiring considerations Use the following chart for interfacing a Q Grad pump s serial communications port to either a 25 pin or a 9 pin COM port on an IBM PC type computer Pump RJ11 Signal IBM DB25 IBM DB9 gt 1 6 Ground 7 DSR 20 RXD TXD DTR a Jumper pins 4 5 and 8 on DB25 b Jumper pins 1 7 and 8 on DB9 3 2 2 Hand Shaking The Q Grad pump uses hardware handshaking Each pump monitors the DSR input and disables the DTR output when the pumps are in refill
3. follows The tray must be oriented as drawn the marking PRSR will be at the top of the fuse tray front The clip must be oriented as shown with the finger at the center pointing toward the front of the tray Do not bend this finger or the required connections will not be made Grasping the clip only at the sides slide it down into place on the tray The base of the clip will insert into the cavity in the tray when it is placed in the correct position 6 Place one fuse into the compartment in the side of the fuse tray opposite the side with the clip The fuse rating must be as shown on the back of the pump cabinet A 115V 1 1 4 long fuse will extend for the full length of the tray A shorter 20 mm long 230V fuse must be placed fully to the rearmost end of the tray closest to the metal tabs extending from the tray or contact will not be made and the unit will not operate Both the clip and the fuse must be present in their proper places or the unit will not operate 7 With the clip and proper fuse in the tray orient the tray with the fuse at the side and the 115V marking at the top as shown in Figure 2 2 and insert it with the metal tabs first into the top of the power entry module until it is firmly seated in place It may be necessary to squeeze the fuse at the rear to permit it to enter the power module housing If the clip and fuse are on the incorrect sides of the tray it will not be possible to place the tray into the power en
4. nawa bae e ain 2 10 BS OPERATION iii Acid 3 1 O N 3 1 31 1PAMO PUEJO VAIVO A A A ee eee ak 3 1 3 2 Rear Panel Remote INpUlS occocnnicconincccccnricr NGNE an aai angan GG ANANG SANGET aan aeea raaa EEA RaRa EEE GEN KEN Sa 3 1 3 2 Hardware mplementalon licita la eae 3 2 32 2 HANG SNAKING esses RO aka a Nai kana 3 3 J 2 3 GOmmand Int rpr ter edie caved Esa d aiana aaao aaa saa sebiexalsdedestuavsianeestdresanvescaees 3 3 3 3 Q Grad CONTO LO pt di 3 6 3 3 1 Keypad CONTO ii A A A a 3 6 3 3 2 PC based Software for Pump CONTIOI sccccscccescesencecenseseneeceneeseneeseaeessneeseaeeseaeeseaaeseaeecsasessaeeseasseaeessaesseeetenees 3 8 3 3 3 Software Control UPgrade s ass tas a Te a TEGAN aa 3 10 3 39 0 HPEC System Control Option ii Kg naan eileen iol Aaah 3 10 4 THEORY OF OPERATION isinan aaa ar ada kada a pi nangga pan aa aa an ad aaa aa pasan da aa aga pi aaa ga da Anaa ag ea pa sedate aaa aa dak da 4 1 4 1 Mechanical Operation icc 5555555865955565 95K9 gg anganan a ANNA 4 1 4 4 4 Liguid System FlOw Path sadasane sana et As Danan gan ga aa pana aane A dh wind ad saga naga gan 4 1 AL PUMP CY CIO ia naga aana a fa Dad gan a aa aaa ga nah AK A ga they A Ba ait rast ga D aa Nk es apaa SS en 4 1 4 1 3 Ple Damping ass aaa sangan nia etapa Ata A A AA einer ae 4 1 A 2 Electroni Control si cicict iste cheats anda alii 4 2 4 2 1 Microprocessor CONO iii taa 4 2 42 2 Power SUDDIV ii A A cal lenin A ie eae oe 4 2 4 2 3 Remote Inte
5. p n WI D2K2053101 provides quaternary gradient control for the Q Grad 3 6 a ai AA a a 51 System E control Unit 7 4 SCU470 The keypad has the capability to store 10 methods of approximately 100 lines each it allows the user to link methods and it is capable of external start by TTL or contact closure input from an injector autosampler or external LC device In addition the keypad will allow the user to set pressure limits for the pump and will shut down all pumps when a pressure fault is detected Priming of the pumps can also be done from the keypad The SCU470 kit contains 4 pump cables an external start cable a PC cable power cable and user manual The PC cable would be used to link the SCU470 to the serial port of a PC to allow modifications of the firmware in the field With the help of the SCU470 CC program provided on a CD the user may make minor modifications to the firmware such as number of methods and the maximum flow rate capability The latter feature is useful for control of other SSI pumps Installation Instructions 1 Connect solvent lines from appropriate reservoirs to the individual pump heads using the 1 8 TFE tubing and fittings provided Set up the remainder of the components of the HPLC system the pump outlet line goes to the injector or autosampler the fluid path then goes to the column the post column unit if any the detector and finally to waste For the initia
6. particles will interfere with the reliable operation of the piston seals and check valves Solvents in which buffers or other salts readily precipitate out will need to be filtered more often After filtration the solvents should be stored in a closed particulate free bottle 2 4 4 Solvents With Harmful Effects Except for PEEK pump heads all portions of the Q Grad pump that contact mobile phase are manufactured of type 316 stainless steel sapphire ruby or fluorocarbon polymer Some of these materials are extremely sensitive to acids including some Lewis acids and acid halides Avoid using solvents that contain any amount of hydrochloric acid Some solvents you should specifically avoid are Aqua Regia Hydrobromic Acid Bromine Hydrochloric Acid Chlorine Anhydrous Hydrofluoric Acid Copper Chloride Hydrofluorsilicic Acid Ferric Chloride Hydrogen Peroxide Ferrous Chloride Iodine Freon 12 wet Mercuric Chloride Guanidine In addition some users of HPLC systems have observed that chloroform and carbon tetrachloride slowly decompose to liberate hydrochloric acid which as noted above attacks stainless steel Do not leave these solvents in the systems for a prolonged period You may also want to avoid ammonium hydroxide Although ammonium hydroxide will not harm the pump itself it is likely to damage the stator and rotor in injection valves The use of THF Methylene Chloride and or Concentrated Sulfuric Acid is to be avoid
7. single serial port of a Windows PC 95 98 NT The software for pump control is very user friendly and intuitive it allows the user to build an unlimited number of gradient methods and also provides direct control of each pump The Gradient Graphic Editor GGE_ tool present in the software allows the user to build gradients with a few clicks of the mouse A Flow Calibration Wizard allows the user to apply corrections to the flow at each pump head to account for solvent compressibility effects In addition the capability for priming pumps external start by TTL contact closure setting pressure limits and modifying maximum flow rate setting is also provided with this software 3 8 Q Grad with Software Control through MPX box The pumps communicate with the PC via an MPX interface box RS 232 cables link the pumps to the MPX interface and a single RS 232 cable with DB 9 connector links the MPX to the PC The software package p n WI QGS includes 4 pump cables MPX interface PC cable external start cable power cable software CD hardware key for parallel port and manual The hardware key allows the user to move the program to different PCs without the key attached all features are still active except for actual communication with the pumps 1 e the program functions as a demo Installation Instructions 1 Connect solvent lines from appropriate reservoirs to the individual pump heads using the 1 8 TFE tubin
8. 10 32 cone seat for the 1 16 outlet tubing The inlet check valve must be connected at the larger opening in the pump head See Figure 5 5 If the piston and flushing seals have been removed insert new seals as described in Section 5 2 3 then continue with Section 5 2 5 to replace the pump head 5 5 Figure 5 5 Check Valves Note On SS detail Double ball and seat check valve shown depending on model your pump may have single 5 2 3 Replacing Piston Seals Lower than normal pressure pressure variations and leaks in the pumping system can all indicate possible problems with the piston seal Depending on the fluid or mobile phase used piston seal replacement is often necessary after 1000 hours of running time 5 6 Each replacement seal kit contains one seal one backup washer one self flush seal one non flush guide bushing two seal insertion removal tools and a pad to clean the piston when changing the seal 5 2 3 1 Removing the Seals 1 Remove the pump head as described in Section 5 2 1 Use caution so as not to damage the sapphire piston 2 Insert the flanged end of the seal insertion removal tool into the seal cavity on the pump head Tilt it slightly so that flange is under the seal and pull out the seal CAUTION Using any other tool will scratch the finish 3 Repeat the procedure for the low pressure seal in the flush housing 4 Inspect and if necessary clean the pump head as described
9. 3 The Motor Stall Detector uses a timer to determine if the cam shaft has stopped turning or if the refill switch is defective If the Motor Stall Detector has been enabled see Section 3 2 3 and the cam shaft stops turning or the refill switch stops operating the fault will be detected between the time it takes to complete 1 to 2 cam revolutions One revolution of the cam shaft produces one complete stroke i e a delivery phase and a refill phase The fault is canceled by sending a stop ST command via the serial communications port on the back panel 5 MAINTENANCE Cleaning and minor repairs of the Q Grad pump can be performed as outlined below NOTE Lower than normal pressure pressure variations or leaks in the pumping system can all indicate possible problems with the piston seal piston or check valves Piston seal replacement could be necessary after 1 000 hours of running time See Section 5 2 3 5 1 Filter Replacement 5 1 1 Inlet Filters Inlet filters should be checked periodically to ensure that they are clean and not restricting flow A restriction could cause cavitation and flow loss in the pump Two problems that can plug an inlet filter are microbial growth and impure solvents To prevent microbial growth use at least 10 20 organic solvent in the mobile phase or add a growth inhibiting compound If you pump 100 water or an aqueous solution without any inhibitors microbes will grow in the inlet f
10. AA ridea NGANENGNENG stewsteidedevesariesterdacassiebelusieslevesicventecesversdtetisccotane 5 11 9 7 LUDFI CANON A A T T A E E A 5 12 5 8 FUSE ReplaceMentt ccsesccsessssesssssscesssssnenseseseesesencessscanessesennenssusnesseseneeseseanessseccenseseanessssessssencessseanessusanensseoensess 5 12 5 9 Battery REplaceMeni sseecccscsercsesseesecsensssesensnsccsenensesensesecsnesssccenessesensessesensssesnanssesensessenssasenesesesenssssesenssenennenens 5 12 6 LIST OF REPLACEMENT PARTS iii ida di adicta 6 1 11 1 INTRODUCTION This operator s manual contains information needed to install operate and maintain the Q Grad Quaternary Gradient Pump 1 1 Description of the Q Grad Pump The Q Grad high performance liquid chromatography HPLC pump is designed to provide high pressure quaternary gradients for HPLC applications Four pumps in a single cabinet are controlled with a keypad module or a PC with supporting software The flow rate of each head on a Q Grad pump fitted with standard 5 mL pump heads can be set in 0 001 mL increments from 0 001 to 5 000 mL min Pump heads are available in type 316 stainless steel or biocompatible metal free PEEK The low pulsation flow produced by the reciprocating single piston pumps are achieved by using an advanced rapid refill cam design programmed stepper motor acceleration and an internal pulse damper 1 1 1 Pump Features The Q Grad Pump Features four Bioclean or type 316 st
11. Assembly with Bushing 2 5 3 Self Flush Tubing Connections Only one inlet and one outlet connection is required for the four self flush heads This is accomplished by interconnecting the heads in series as shown in Figure 2 5 below Figure 2 5 Self Flush Tubing Arrangement CAUTION Since each self flush head has its own inlet and outlet check valve be sure each head is oriented in the same direction The inlet is on the bottom and the outlet is on the top One backward valve will stop the flushing solution flow 2 7 2 5 4 Inlet Tubing and Filters The inlet tubing used in the Q Grad pumps all head types is 0 085 ID x 1 8 OD All inlet lines are supplied in a 36 91 cm length and are made of a Teflon based material Use a 0 5 micron slip on inlet filter Install in line shut off valves filter assemblies supplied in start up kit into inlet check valves as shown in figure below eo A X f NG n 2 5 5 Outlet Tubing Outlet tubing not supplied with the pump should have a 1 16 outer diameter It is available in type 316 stainless steel or PEEK Tubing with a 0 020 inner diameter is normally used before the injection valve Tubing with a 0 010 inner diameter is normally used after the injection valve The tubing must be cut squarely with no burrs The tube itself should not be crimped and the center hole must be open A tubing cutter is recommended for cutting stainless steel tubing PEEK
12. In addition the pump will not transmit if the DSR input becomes active 3 2 3 Command Interpreter The Q Grad pump s command interpreter receives and responds to command packets The pump will not send a message except when prompted and it will send a response to every valid command as described below The response to an invalid command is Er Each command is characterized by a unique two letter command code and only one command can be issued per line Case is not important that is the command codes PR Pr pR and pr are all equivalent Response strings sent by the pump are terminated by the character The command packets are listed in Table 3 1 If the pump s response is Er send a to clear any characters which may be remaining in the command buffer The pump will automatically clear all characters in the command buffer after one second elapses from the time at which the last character of an incomplete command was sent The following commands are valid only for pump head A PR Upxxxx and LPxxxx CAUTION If operating the pump with fewer than four pump heads always connect the keypad controller or PC with supporting software to pump A to monitor the system pressure See Section 2 5 7 3 3 Table 3 1 Q Grad Pump Commands FLxxx OK Sets the flowrate to x xx mL min where the range is fixed for the pump head size i e for 0 01 to 9 99 mL min xxx 001 to 999 FOxx
13. Q Grad Quaternary Gradient Pump Operator s Manual 90 2321 Rev E SSI Labaltiance Scientific Systems Inc 349 N Science Park Rd State College PA 16803 Phone 800 345 5557 814 234 7317 Fax 814 238 6072 www laballiance com Table of Contents INTRODUCTION iio A AA AE aa dah A a DA DEE a pa A Ba ANAA Kau aa anana an 1 1 1 1 Description of the G Grad Pump ssss5s 588515575 Sa 7455 aa nagan ceestsse casey a a 1 1 Teta Pump FS Si A aga ag aa aga a a a A akeh naa a gg Tenant dh aa a 1 1 TA Wetted Malena A aa ga Egen e a nag ia LE a a ag ana eee ee 1 2 1 193 Self Flushing Pump Head S ss ianed ga aaa ag EA aab angan in aig deine tant ali gi ah aha a PEN 1 2 1 1 4 Self Fl sh and Seal Les ss ce A A eee ee Ae a eee 1 3 1 2 Specifications for the Q Grad PUMP sseccccersseesscenssennssnenssennssnensseansscensssnasacenssenassnensseaasacanssensseanessnnsseanesenesseans 1 3 2 INSTALLATION coi a ii 2 1 2 1 UNPACKING and INSpectiON ommmmcccinincinicccincar EH a NEKENAKEN NGONE EEN GNAN NEGEN NE EEN NENENGEA EN Aung ieaiaia 2 1 2 2 LOCALION ENVIFONMENE sseecscsennessesenenscennesensneesesensessesenessesensessusonsssesanessseaneessesnssssesenesseaneseseneessesenesseenenenessneessess 2 1 2 3 Electrical Connect ah 2 1 2 3 1 Converting from 115Vac to 230 Va hanina Ad a e aie ga a ann 2 1 2 3 2 Converting from 230Vac to 115 V ae sii teed hae ag AE a Ag a a NAN E E aise aie adage ett Ei 2 3 2 4 Solvent AAA PP Eae EEA ENENGENA KE LENENG be
14. a aaa b c d e f g h i j k Reads the current pump setup where m n o p q a aaa or aa aa Flowrate in mL min b Run status 0 stopped 1 running a aaa or aa aa c or cc Pressure compensation c or cc d Pump head type see RH command e Pressure Board present 0 otherwise 1 f External control mode 0 frequency 1 voltage g 1 if pump started and frequency controlled else 0 h 1 if pump started and voltage controlled else 0 i Upper pressure limit fault 0 no 1 yes j Lower pressure limit fault 0 no 1 yes k Priming 0 no 1 yes Keypad lockout 0 no 1 yes m PUMP RUN input 0 inactive 1 active n PUMP STOP input 0 inactive 1 active o ENABLE IN input 0 inactive 1 active p Always 0 q Motor stall fault 0 no 1 yes Resets the pump configuration to its default power up state Clears all characters from the command buffer 3 3 Q Grad Control Options Control of the Q Grad pump is achieved using the four RS 232 ports corresponding to pumps A B C and D on the rear panel Various user selectable pump control options are available ranging from a compact keypad controller to a high end software package for an entire HPLC system A summary of the main features of each control option is presented below with a brief installation procedure Refer to the appropriate manual for details 3 3 1 Keypad Controller The LabAlliance SCU470 Keypad Controller
15. a back pressure and flow rate listed under PHASE 2 below PHASE 1 30 min PHASE 2 15 min Pump Head Type Pressure Flow Rate Pressure Flow Rate 10 mL 1000 psi lt 3 mL min 3000 4000 psi 3 4 mL min SS or PEEK 5 mL 1000 psi lt 2 5 mL min 1500 psi lt 5 mL min SS or PEEK 5 4 Check Valve Cleaning and Replacement Many check valve problems are the result of small particles interfering with the operation of the check valve As a result most problems can be solved by pumping a strong solution of liquid laboratory grade detergent through the check valves at a rate of 1 mL min for one hour After washing with detergent pump distilled water through the pump for fifteen minutes Always direct the output directly to a waste beaker during cleaning If this does not work the check valve should be replaced 5 9 CAUTION When removing the check valves keep them in the orientation shown below the entire time they are not installed in the pump head The assemblies may fall apart parts may be lost and they may not operate properly when re assembled 5 5 Pulse Damper Replacement 5 5 1 Removing the Pulse Damper WARNING There are potentially lethal voltages inside the pump case Disconnect the line cord before removing the cover Never bypass the power grounds 1 Make certain that the system has been depressurized Unplug the power cord and remove the cover 2 Disconnect the inlet and outlet tubing from the pulse d
16. ack into the mobile phase within four hours Solvent mixtures using water and organic solvents like methanol or acetonitrile hold less dissolved gas than pure solvents Sparging to reduce the amount of dissolved gas is therefore particularly important when utilizing solvent mixture Even with sparging some out gassing may occur A back pressure regulator installed after the detector flow cell will help prevent bubbles from forming and thus limit baseline noise CAUTION Always release pressure from the pump slowly A rapid pressure release could cause the pulse damper diaphragm to rupture 2 4 2 Cavitation 2 4 3 Filtration Cavitation occurs when inlet conditions restrict the flow of solvent and vapor bubbles are formed during the inlet stroke The key to preventing cavitation is to reduce inlet restrictions The most common causes of inlet restrictions are crimped inlet lines and plugged inlet filters Inlet lines with tubing longer than 48 120 cm or with tubing of less than 0 085 2 mm ID may also cause cavitation Placing the solvent reservoirs below the pump level also promotes cavitation The optimal location of the reservoirs is slightly above the pump level but it is adequate to have them on the same level as the pump Solvent filtration is good practice for the reliability of the Q Grad pump and other components in a HPLC system Solvents should always be filtered with a 0 5 micron filter prior to use This ensures that no
17. ainless steel pump heads independently controlled for accurate gradients Incorporates a diaphragm type pulse damper which reduces pulsation in the system by as much as 90 and acts as a gradient mixer Includes an isolated pressure transducer i e the transducer adds no dead volume Automatically turns the pump OFF if the system pressure exceeds the maximum pressure limit determined by the pump head type 6000 psi for stainless steel pump heads 5000 psi for PEEK pump heads The operator may program upper and lower pressure limits within the maximum range set by the pump head type Integrated prime purge valve Self flushing pump heads Microprocessor advanced control Digital stepper motor design prevents flowrate drift over time and temperature variations Back panel RS232 serial communications ports for complete control and status monitoring of each pump head 1 1 e Real time system pressure reading through the RS232 port for pump A 1 1 2 Wetted Materials Pump heads check valve bodies and tubing are made out of type 316 stainless steel or PEEK depending on version ordered Other materials common to either stainless steel or PEEK models are synthetic ruby and sapphire check valve internals and piston and fluorocarbon pulse damper diaphragm 1 1 3 Self Flushing Pump Heads Self flushing pump heads provide continuous washing of the piston surface without the inconvenience of a manual flush or gravity f
18. amper 3 Disconnect the transducer cable plug from the circuit board for pump head A 4 Remove the two screws that secure the pulse damper to the pulse damper support bracket 5 Remove the pulse damper Inlet and Outlet Tubing Mounting Screws Pulse Transducer Damper Cable Plug 5 10 5 5 2 Pulse Damper Refurbishing Refurbishing the pulse damper is a time consuming procedure You may want to return the pulse damper to have it rebuilt Do not attempt to refill or refurbish the pulse damper 5 5 3 Pulse Damper Installation 1 3 Insert the pulse damper below the support bracket with the transducer pointing down and secure the pulse damper to the bracket with the two mounting screws Attach the transducer cable plug to the circuit board labeled PRESSURE BOARD for pump head A The male socket is labeled TO PRESS XDUCER Connect the inlet and outlet tubing to the pulse damper 5 6 Cleaning the Pump 1 2 7 Disconnect the column inlet tube from the column Direct the column inlet tube the tube from the injector outlet to a waste beaker Set the flow rate to maximum Turn the injector to the INJECT position Pump 100 methanol or isopropanol through the pump and injector for 3 minutes Pump 100 filtered distilled water through the pump and injector for 3 minutes For stainless steel flow paths proceed to Step 7 For PEEK flow paths skip to step 10 WARNING Use st
19. andard laboratory procedures and extreme care when handling strong acids and bases Pump a 20 nitric acid water solution through the pump and injector for 3 minutes Flush the pump and injector with 100 filtered distilled water for at least 3 minutes Pump 100 isopropanol through the pump and injector for 3 minutes The pump is now prepared for any mobile phase or short or long term shutdown 5 11 10 If storing the pump for more than 12 hours fill each pump head with 100 isopropanol then seal the inlets and outlet with the supplied plugs 5 7 Lubrication The Q Grad pump has modest lubrication requirements The bearings in the pump housing and piston carrier are permanently lubricated and require no maintenance A small dab of light grease such as Lubriplate 630 AA on the cam is the only recommended lubrication Be sure not to get lubricant on the body of the piston carrier as this can retard its movement and interfere with proper pumping NOTE Keeping the interior of the pump free of dirt and dust will extend the pump s useful life 5 8 Fuse Replacement Six fuses protect the Q Grad pump Two of the fuses are located in the power entry module at the rear of the cabinet The other four fuses are located one on each of the four motor power circuit boards Troubleshooting the fuses is straightforward If the power cord is plugged in and the ON OFF power entry switch is ON and the fan does not run check the two fuse
20. aphragm compressing the fluid in the cavity and storing energy During the pump refill phase the pressure on the diaphragm is reduced and the compressed fluid expands releasing the energy it has stored This helps to stabilize flow rate and pressure The amount of mobile phase in contact with the pulse damper is small only 1 2 mL at 6 000 psi and the geometry used insures that the flow path is completely swept so solvent memory effects are virtually eliminated 4 2 Electronic Control 4 2 1 Microprocessor Control The pump is controlled by hybrid microprocessor circuitry which 1 provides control signals to the motor power board 2 receives signals from the pressure transducer and refill flag and 3 provides external input output and remote control interfacing Firmware programming is stored in an EPROM The motor power board contains programmed logic components which 1 provide suitable motor micro stepping modes 2 allow appropriate motor power adjustment 3 maximize motor power output 4 reduce motor resonance effects and 5 customize motor stepping uniformity A specially shaped cam provides a linear piston displacement with a rapid refill In addition to the cam characteristics the microprocessor optimizes the refill speed for the set flow rate As a result at 1 mL min flow the refill rate is more than five times faster than if the motor operated at constant speed The optimum refill minimizes the resulting pulsat
21. box and the pump pump A to port A pump B to port B etc Optional Connect the external start blue green cable between the S connection at the input flags terminal and the position sensing switch of a manual injector or to the inject start function of an autosampler Turn the computer off and connect the host adapter cable from the Pl interface box at the Host PC connection to the serial port of a PC using the Y connector that is part of the kit Load the software on the PC Turn the pump on power up the P1 box Launch the LA Rack program Under Setup select the Configuration Wizard option that will guide the user through setup of the HPLC system pump detector and autosampler Close and reopen the program to accept the changes The program may take 1 2 minutes to communicate the information to the P1 module Under Instruments select Pump Control Set upper pressure limit in this panel The edited method can be saved as a pump control file pcf and launched into a method file later See manual for details A gradient method may be set up using the gradient mode in the pump panel The edited method can be saved as a pump control file pcf and launched into a method file later See manual for details 3 11 4 THEORY OF OPERATION 4 1 Mechanical Operation 4 1 1 Liquid System Flow Path The flow path of the Q Grad pump starts at the inlet of ea
22. c NENEK RAE RE REE ETAT EEEE 2 4 2 4 1 Solvent Out gassing and Sparging u ccccceccccececeneeeseceeseeeeseaeesneeesaecsaaeesaeesaaessaessaeeesaaeseaeeesaeessaesseaeesiaeeseaeeneees 2 4 2 4 2 CAVILALON sive Sortie tiger ia oe ee Sain BA es is ea ein ae BAG nee 2 5 2 43 FINPALION asana nti ee pie ee agit We aie ented a ei is 2 5 2 4 4 Solvents With Harmful Effects ss sas srira ah ga an aga caca 2 5 2 5 Instrument InstallatiON oonnnnmmiccccnnncicccc tanei ediad tee iodine deidan e ea aehi droni ed ti riid idr dasini radin 2 6 2 9 1 Mobile Phase ROSCrvoirs esmerin eein ie 2 6 2 5 2 Self Flush Sl a EVER 2 6 2 5 3 Self Flush Tubing C0ONNGGHOh Ss saksa sadasa seii aia bi 2 7 2 5 4 Inlet Tubing AA ie een i Anse ei ee 2 8 2 5 5 Outlet TUDING A edt Aleta a deel eres 2 8 2 5 6 Priming the Pump and the Flushing Lines oocoonicciinniccccnn mece 2 8 2 5 7 Pressure Fault System Proteeton sasasi aisi cece een ierra EEE EAE EEA EEDA EAD Een SEE nena nee 2 8 2 5 8 Long Term Pressure Calibration Accuracy ooooccnocinnccnonicionononcnonnnannr ronca 2 9 2 6 Preparation for Storage Or SHIPPING s sccsercscecccsenescnnsssenessensssnnesennsssenesenessunnasenesssanasesessaeeasenessenessneassenessnenseeness 2 9 LON ISOPFOPAMO FIUSH sss 5 05 satanah seek Nga a Na aaa eased tae cp E aa A GAE E a ey Kg a cose ide das ask a dave hse eats 2 9 2 6 2 PACKAGing fOr SHIPPING sis fetes cence ka Ga aa a a NGA a na asec aan dace senna a a aa pew kaa a a aa a nan Sak eng nan
23. ch pump head pumps through the pump heads and combines in the 5 way prime purge valve into the pulse damper then finally through the bulkhead filter and out the front panel of the pump 4 1 2 Pump Cycle The pump cycle consists of two phases the pumping phase and the refill phase During the pumping phase the pump piston moves at a constant linear speed driven by a specially shaped cam which is in turn driven by the motor using a toothed belt drive This results in a constant stable flow from the pump at high pressure At the end of the pumping phase the pump enters the refill phase The cam is shaped so that the piston quickly retracts refilling the pump head with solvent The piston then moves forward again as the pumping phase begins Since the output flow completely stops during refill a pulse damper is necessary to provide some of the lost flow see 4 1 3 below In addition the motor speed is adjusted by the microprocessor to facilitate an efficient refill phase The combination of increased motor speed and the rapid refill design of the cam generates refill times of less than 12 5 of the pump cycle the refill time at 1 mL min is less than 5 of the pump cycle 4 1 3 Pulse Damping The diaphragm type pulse damper consists of a compressible fluid isopropanol held in an isolated cavity by an inert but flexible diaphragm During the pumping phase of the pump cycle the fluid pressure of the mobile phase displaces the di
24. control using the Dial A Mix mode allows for quick priming or flow control of a specific pump A unique Configuration Wizard helps the user to configure in various pump detector and autosampler devices to set up the System Configuration Communication with the pumps is via RS 232 cables connected to the Pyramid Pl interface box The Pl box is an external hardware device to which various LC components are connected communication between the P1 interface module and a serial port of the PC is established through a special cable with a DB9 connector The Unity package SD PC UN includes 4 pump cables 2 detector autosampler digital cables 2 detector analog input cables external start cable Pl interface box with PC cable software CD Computer with Office 2000 and a monitor The same Unity package without PC monitor is p n AC 950 150 Installation Instructions 1 Connect solvent lines from appropriate reservoirs to the individual pump heads using the 1 8 TFE tubing and fittings provided Set up the remainder of the components of the HPLC system the pump outlet line goes to the injector or autosamper the fluid path then goes to the column the post column unit if any the detector and finally to waste For the 3 10 initial priming you may connect the pump outlet directly to waste Refer to the appropriate LC component manual for specific details Connect the RS 232 cables between the Unity P1 component interface
25. e battery is all the way into place It must contact the base of the battery socket Replace the circuit board Screws Replace the cover to the unit Plug the unit back in 5 13 You Notice Uneven pressure trace Pressure drops Pump shuts OFF No flow out the outlet check valve Uneven pressure trace Pressure drops Fluid between the pump head and the chassis Pump makes a loud clanging or slapping noise intermittent contact with cam Blue dye in mobile phase Pump runs for 50 pump strokes then shuts down Pump shuts down after run is called even with no column connected Pump runs to maximum pressure and shuts down No power when pump turned ON Fan does not run Pump motor does not run but fan is running PEEK fittings or components leak Self flush heads leak flush solution Quick Guide to Problem Solving This May Mean Bubble in check valve Leaks in system Dirty check valve Bad check valve Leaks in system The piston seal s are worn Piston carrier is catching in piston guide Pulse damper diaphragm has burst Lower pressure limit is activating Clog in fluid system Blown fuses in the power entry module Blown fuse on the motor power circuit board You cannot force PEEK parts with interference to seal by brute force tightening Flush area not sealed Possible Cause Solvent not properly degassed Fittings are not tig
26. ed with the PEEK version pumps 25 Instrument Installation 2 5 1 Mobile Phase Reservoirs The mobile phase reservoir should be placed at the same level or slightly higher than the pump never below the pump and the inlet tubing should be as short as practical These steps minimize pressure losses on the inlet side of the pump during refill and help to avoid bubble formation These steps are particularly important when using high vapor pressure solvents hexane methylene chloride etc Mobile phases should be degassed filtered and covered See Section 2 4 2 5 2 Self Flush Solution Self flush heads require 250 500 mL of 20 methanol in water as a flushing solution A pH indicator that will indicate the concentration of salts in the solution is recommended as a reminder to change the solution This flush solution should be replaced with a fresh solution weekly to avoid frequent pump maintenance CAUTION If you do not use the self flush feature of this pump you must carefully remove the self flush seal with the seal tool provided and place the seal into the guide bushing bag Replace the flush seal with the guide bushing provided If this is not done low flowrates excessive noise and shortened pump life will result See illustration below Figure 2 4 a 8 UA Wa i AN GY Pd gt CA EN YA KA a WA MA ZA A Wa f NT A AN KZ a Self Flush Assembly Non Flush Assembly Figure 2 4 Self Flush Assembly with Seal vs Non Flush
27. eed arrangement The self flushing pump head uses a secondary seal and set of check valves to create a continuous and positive flow in the area behind the high pressure pump seal The flushing solution washes away any buffer salts that have precipitated onto the piston If not removed these precipitates can abrade the high pressure seal and cause premature seal failure leakage and can possibly damage the pump Flush Solution Pumped flow of Washes Piston m Flush Solution Piston jen Movement gt AS Sapphire Secondary bil Piston Self Flush Seal obile Phase Primary High Pressure Seal and Backup Washer Figure 1 1 Self Flushing Pump Head 1 2 1 1 4 Self Flush and Seal Life It is recommended that the Self Flush feature be used to improve seal life in a number of applications In particular as stated above if pumping Buffers Acids Bases or any inorganic solution near saturation the pump should utilize the Self Flush feature With every piston stroke an extremely thin film of solution is pulled back past the seal If this zone is dry without use of Self Flush then crystals will form with continuous operation which will ultimately damage the seal Water or water IPA mix are good choices for the flush solution Consult the factory for specific recom
28. es not have the correct voltage configuration convert the pump using the procedure below To change the voltage setting a small flat blade screwdriver is the only tool required proceed as follows 2 3 1 Converting from 115Vac to 230Vac 1 Remove the power cord from the power entry module see Figure 2 1 located in the rear of the pump Figure 2 1 Power Entry Module 2 Using a small flat blade screwdriver carefully pry out the power entry module cover at point A Figure 2 1 The hinged cover will swing down as shown in Figure 2 2 Fuse Tray Hinged Cover Figure 2 2 Removing the Fuse Tray 3 Using a small flat blade screwdriver carefully pry out at the top of the fuse tray and carefully remove the tray as illustrated in Figure 2 2 4 Holding the fuse tray in the exact orientation shown in Figure 2 3 observe the small clip shown Grasping at the two sides of the clip gently remove it from the tray assembly as shown in Figure 2 3 5 Remove the fuse present in the fuse tray by gently lifting it up at the rearmost end of the tray where the metal tabs extend and removing the fuse from the retaining clip Rearmost end of Fuse Tray Figure 2 3 Removing the Clip from the Fuse Tray 6 Place two fuses into the two compartments in the fuse tray The fuse ratings must be as shown on the back of the pump cabinet The 115V 1 1 4 long fuses extend for the full length of the tray The shorter 230V 20 mm long fu
29. g and fittings provided Set up the remainder of the components of the HPLC system the pump outlet line goes to the injector or autosampler the fluid path then goes to the column the post column unit if any the detector and finally to waste For the initial priming you may connect the pump outlet directly to waste Refer to the appropriate LC component manual for specific details Connect the RS 232 cables between the MPX pump interface box and the pump pump A to port A pump B to port B etc Optional Connect the external start cable of the MPX box to the position sensing switch of a manual injector or to the inject start function of an autosampler Turn off the PC Connect the hardware key to the parallel port of the PC Connect the serial cable from the com port of the PC to the MPX PC connection 5 Turn on the PC and load the software 6 Open the LAGradControl program and under System select Solvent Delivery System Enter the COM port of the PC to which the MPX box is connected Check mark the pumps being used and enter pressure limits flow information click OK when complete close and reopen program to accept the changes Priming may be done directly by clicking on the syringe icon that corresponds to each pump Alternately use Direct Control to set a flow rate and independently run or prime each pump If solvents of widely varying compressibility are being used the Flow Cal
30. he pump Move the pump head straight out from the pump and remove it from the piston Be careful not to break or damage the piston Also remove the seal and seal backup washer from the piston if they did not stay in the pump head 10 Carefully separate the flush housing from the pump Move the flush housing straight out from the pump and remove it from the piston Be careful not to break or damage the piston Also remove the self flush seal from the piston if it did not stay in the flush housing Figure 5 1 Stainless Steel Self Flushing Pump Head Assembly 5 2 e S KU NI i SV Figure 5 2 Stainless Steel Non Self Flushing Pump Head Assembly Figure 5 3 Bioclean PEEK Self Flushing Pump Head Assembly 5 3 Figure 5 4 Bioclean PEEK Non Self Flushing Pump Head Assembly 5 2 2 Cleaning the Pump Head Assembly NOTE If you choose to remove the piston seal or self flush seals you should have a new set on hand to install after cleaning It is not recommended that you reinstall used piston or self flush seals since they are likely to be scratched and damaged during removal and would not provide a reliable seal if reused If you decide to remove the seals use only the flanged end of the plastic seal removal tool supplied with the seal replacement kit and avoid scratching the sealing surface in the pump head See Section 5 2 3 for seal replacement instructions 1 Inspect the piston sea
31. ht Mobile phase not properly filtered Particles from worn piston seal caught in check valve Plugged inlet filter Fittings not tight Long usage time since last seal change Salt deposits on seal especially if buffered aqueous mobile phases are used without the self flush head Cap nut screws on the pump head are loose Seal s are worn Piston guide is worn Salt build up on piston carrier from use of buffers Excess lubricant on piston carrier Sudden pressure drop when purging system Mobile phase is not properly filtered Particles from worn seal trapped in the system e g tubing filters injection valve column inlet Power surge Internal short Power surge Internal short Film of fluid between surfaces Salt crystals between surfaces Scratches in mating surfaces Large Size 016 O ring is flattened and no longer seals Head not sufficiently tightened Scratches in mating surfaces Leaky self flush seal 5 14 You Should Check to be certain that mobile phase is properly degassed Check connections for leaks by tightening fittings Prime the system directly from the outlet check valve Clean or replace the check valves See Section 5 4 Clean or replace inlet filter See Section 5 1 1 Check all connections for leaks Replace piston seal See Sections 5 2 and 5 3 Check the piston for salt deposits Clean as necessary See Section 5 2 4 Check cap nut screws on p
32. ibration selection under System will bring up a wizard that will help the user with entering newly calibrated flow values for the individual pumps This will automatically adjust the flows associated with the specific pumps when running the gradient program 3 9 9 A gradient method may be built by clicking the gradient table icon and entering the desired steps Alternately the graphic editor tool can be used to build a method by clicking the appropriate solvent bottle icon See manual for details 3 3 3 Software Control Upgrade Users who already have the SCU470 keypad control option and also wish to have the PC based software control for the Q Grad pump should order the upgrade kit p n WI D2K2062001 3 3 4 HPLC System Control Option Q Grad pump control can be performed through Unity the complete LabAlliance HPLC system control package for pumps detectors autosamplers and data collection The Unity Control Panel allows efficient communication with pumps and other LC devices The GUI pump control panel permits the user to quickly build a method to set pressure limits and to launch a pump control file directly into a method file Three modes of pump operation are available from the interface panel Isocratic Gradient and Dial A Mix In gradient mode a convenient drop down gradient table may be used to edit a gradient method even while the pumps and other components are in equilibration phase Independent pump
33. ilized by the controller for system over pressure protection 2 5 8 Long Term Pressure Calibration Accuracy This note applies if your pump is equipped with an electronic pressure transducer The transducers zeroed and calibrated over their specified range at the factory Over the life of the pump some drift may occur For example it is typical for the zero to drift lt 10 p s i after about 1 year of operation i e with no back pressure on the pump a reading of 1 9 p s i may be displayed A similar drift may also occur at higher pressures and are typically less than 1 e g lt 50 p s i at 6 000 p s i back pressure If pressure calibration and or drift is a concern consult the factory The pump can be shipped back to SSI for recalibration Alternatively written calibration and zero reset procedures are available Consult the factory to receive these instructions 2 6 Preparation for Storage or Shipping 2 6 1 Isopropanol Flush Disconnect the outlet tubing from the pump Insert the four inlet filters in isopropanol Open the prime purge valve and use a syringe to draw a minimum of 50 mL Close the prime purge valve and pump a minimum of 5 mL of isopropanol to exit Leave the inlet tubing connected to the pump Place the inlet filter in a small plastic bag and attach it to the tubing with a rubber band Seal the outlet port with the shipping plug 2 6 2 Packaging for Shipping CAUTION Re package in the original carton if possib
34. ilter over time even if you make fresh solution every day Always use well filtered HPLC grade solvents for your mobile phase 5 2 Changing Pump Heads 5 2 1 Removing a Pump Head CAUTION The sapphire piston is fragile Twisting the pump head during removal can cause the piston to break Closely follow instructions during head removal and replacement to avoid breakage As a guide to pump head assembly the standard pump heads are shown in Figures 5 1 through 5 4 All of the Q Grad pump heads have a similar arrangement 1 Turn OFF the power to the Q Grad pump 2 Remove the inlet line and filter from the mobile phase reservoir Be careful not to damage the inlet filter or crimp the Teflon tubing CAUTION Check that the knurled nuts at the front of the pump head are secure before removing any tubing from the pump head 3 Remove the inlet line from the inlet check valve 4 Remove the outlet line from the outlet check valve 5 1 5 Remove inlet and outlet self flush check valves 6 Momentarily turn ON the Q Grad pump and quickly turn OFF the power upon hearing the refill stroke This reduces the extension of the piston and decreases the possibility of piston breakage 7 Unplug the power cord 8 Carefully remove the two knurled nuts at the front of the pump head CAUTION Use care when removing the pump head Twisting the pump head can cause the piston to break 9 Carefully separate the pump head from t
35. in Section 52 2 5 2 3 2 Cleaning the Piston 1 Once the pump head and self flush housing are removed gently remove the seal back up plate by using either a toothpick or small screwdriver in the slot on top of the pump housing 2 Grasp the metal base of the piston assembly so that you avoid exerting any side load on the sapphire rod and remove the piston from the slot in the carrier by sliding it up 3 Use the scouring pad included in the seal replacement kit to clean the piston Gently squeeze the piston within a folded section of the pad and rub the pad along the length of the piston Rotate the piston frequently to assure the entire surface is scrubbed Do not exert pressure perpendicular to the length of the piston as this may cause the piston to break After scouring use a lint free cloth dampened with alcohol to wipe the piston clean 4 Grasp the metal base of the piston assembly and insert it into the slot in the piston carrier until it bottoms in the slot 5 2 3 3 Replacing the Seals 1 Place a high pressure replacement seal on the rod shaped end of the seal insertion removal tool so that the spring is visible when the seal is fully seated on the tool Insert the tool into the pump head so that the open side of the seal enters first facing the high pressure cavity of the pump head Be careful to line up the seal with the cavity while inserting Then withdraw the tool leaving the seal in the pump head 5 7 When yo
36. ion while avoiding cavitation effects in the solvent entering the pump head The flow rate of any high pressure pump can vary depending on the operating pressure and the compressibility of the fluid being pumped The pump is calibrated at 1000 psi using a 80 20 mixture of water and isopropanol The pulse damper of the Q Grad pump has a built in pressure transducer which senses fluid pressure of the system The output is sent to the microprocessor circuit of Pump A This pressure information is compared with the user set upper and lower pressure limits to control pump shut off if the limits are exceeded 4 2 2 Power Supply The pump is supplied with the necessary components for voltage reconfiguration Either of the two voltages 115V nominal 100 120 Vac or 230V nominal 220 240 Vac can be changed by the user if necessary see Section 2 3 4 2 3 Remote Interfacing Four RS 232C modular jacks are provided on the back panel See Section 3 2 for information on pump operation via these connections 4 2 4 Motor Stall Detector The motor can stall and create a loud buzzing sound if the flow path connected to the pump s outlet becomes plugged if the pressure exceeds the maximum pressure rating of the pump or if the mechanism jams In the event a motor stall occurs the electrical current being supplied to the 4 2 motor is turned off and the fault status can be determined through the RS232 PI command See Section 3 2
37. is 0 The value must be expressed as four digits i e for 100 PSI xxxx 0100 Valid only for pump A LPXxxx OK Sets the lower pressure limit in PSI The maximum value for xxxx is the current upper pressure limit setting minus 100 the minimum value is 0 The value must be expressed as four digits i e for 100 PSI xxxx 0100 Valid only for pump head A a e T Puts the pump in fault mode and stops the pump immediately OK x y z Reads the fault status where x Motor stall fault 0 no 1 yes y Upper pressure limit fault 0 no 1 yes z Lower pressure limit fault 0 no 1 yes Sets the pressure compensation value where xx the operating pressure in PSI divided by 100 i e for O PSI xx 00 for 5000 PSI xx 50 Table 3 1 Q Grad Pump Commands continued Reads the pressure compensation value in hundreds of PSI i e for O PSI x 0 for 5000 PSI xx 50 Sets the pump head type where x 1 for a stainless steel 10 mL min pump head x 2 for a plastic 10 mL min pump head x 5 for a stainless steel 5 mL min pump head x 6 for a plastic 5 mL min pump head The pump is stopped and the pressure compensation and pressure limits are initialized when the head type is changed Reads the pump head type where x 1 for a stainless steel 10 mL min pump head x 2 for a plastic 10 mL min pump head x 5 for a stainless steel 5 mL min pump head x 6 for a plastic 5 mL min pump head 3 5 OK
38. l cavity in the pump head Remove any foreign material using a cotton swab or equivalent and avoid scratching the sealing surfaces Repeat for the self flush housing Be sure no fibers from the cleaning swab remain in the components The pump head check valves and self flush housing may be further cleaned using a laboratory grade detergent solution in an ultrasonic bath for at least 30 minutes followed by rinsing for at least 10 minutes in distilled water Be sure that all particles loosened by the above procedures have been removed from the components before re assembly 5 4 CAUTION When cleaning check valves be sure that the ball is not against the seat in the ultrasonic bath This may destroy the precision matched sealing surface and the valve will not check CAUTION If removing the check valves keep them in the orientation shown below the entire time they are not installed in the pump head The assemblies may fall apart parts may be lost and they may not operate properly when re assembled 3 If the check valves have been removed tighten the check valves on stainless steel pumps to 75 inch pounds or enough to seal at maximum pressure Do not exceed maximum torque For Bioclean PEEK pumps tighten each check valve firmly by hand Do not go 4 turn past finger tight NOTE The inlet check valve has a larger opening 1 4 28 flat bottom seat for the 1 8 inlet tubing the outlet check valve has a smaller opening
39. l priming you may connect the pump outlet directly to waste For the initial priming you may connect the pump outlet directly to waste Refer to the appropriate LC component manual for specific details 2 Attach the SCU pump cables from the keypad to the appropriate RS 232 port at the back of each pump on the rear panel Ensure the port A is connected to pump A port B to pump B etc 3 7 3 Optional Connect the external start cable of the SCU to the position sensing switch of a manual injector or to the inject start function of an autosampler 4 Connect the power cable of the SCU and ensure the display is active The current Status will show IDLE 5 Scroll down the SCU display with the down arrow until the Prime commands for the individual pumps can be accessed Press the number corresponding to a specific pump as indicated on the display in order to prime that pump head Priming may be stopped at any time by pressing CL or scrolling out of this screen 6 To build a method table and access other user defined parameters press MODE to toggle between the Status screen and PARAMETER entry screen A new time value needs to be entered for each gradient step to be entered Details of building a gradient method may be found in the SCU470 keypad manual 3 3 2 PC based Software for Pump Control The LabAlliance PC based software control option provides quaternary gradient control of the Q Grad through a
40. le If the original carton is not available wrap the pump in several layers of bubble wrap and cushion the bottom top and all four sides with 2 of packaging foam Although heavy an HPLC pump is a delicate instrument and must be carefully packaged to withstand the shocks and vibration of shipment 2 10 3 OPERATION 3 1 Front Panel CS SS Figure 3 1 Q Grad Pump Front Panel 3 1 1 Prime Purge Valve The prime purge valve vents the flow to atmosphere and permits efficient priming of the Q Grad pump When the valve is closed fully clock wise firmly high pressure flow is directed to the Filter Outlet port When the valve is opened counter clock wise one half to one full turn pressure is vented and flow exits through the drain port in the prime purge valve stem assembly Suction with a Luer tip syringe at the drain port will purge air bubbles from the pump and reservoir lines provided there are no open valves to lines downstream at the injector column interface To prime the pump draw about 20 to 30 mL of mobile phase 3 2 Rear Panel Remote Inputs Four RS 232C modular jacks are provided on the back panel A computer with appropriate software or the keypad control module is used as a remote control device for pump operation via these connections 3 1 mer q E p i a So e y o E gt
41. lse damper with transducer Integrated prime purge valve Replacement seal kit for prime purge valve Connecting Tee 1 16 Use with 981016 Nut Description Bioclean PEEK check valve kit for one pump head Bioclean PEEK in line coupling E Z Grip 1 16 gland nut for outlet tubing PEEK E Z Grip 1 16 gland nut for outlet tubing E Z Grip 1 16 ferrule for outlet tubing Pulse damper with transducer Bioclean PEEK integrated prime purge valve Replacement seal kit for prime purge valve Connecting Tee 1 16 Use with 981016 Nut Description Piston seal kit Includes seal 1 backup washer 1 guide bushing 1 self flush seal 1 o ring and 1 tool Pack of 10 piston seals only 5 mL Description Inlet filter 20 micron Replacement filter elements for 060110 pkg 2 Flushing check valve kit Piston assembly 5 mL Low Volume Pulse Damper Factory Repair Must return Pulse Damper 1 A slow blo fuse 120 V 6 1 931618 1 2 A slow blo fuse 240 V 931619 5 A fast blo fuse motor drive 931790 Battery 6 2
42. mendations Another application where Self Flush is highly recommended is when pumping Tetrahydrofuran a k a THR Diethylene Oxide or other volatile solvents such as Acetone Note THF and most solvents are compatible only with all Stainless Steel systems THF will attack PEEK Volatile solvents will dry rapidly behind the seal without the use of Self Flush which will dry and degrade the seal IPA Methanol water IPA mix or water Methanol mix are good choices for the flush solution Consult the factory for specific recommendations 1 2 Specifications for the Q Grad Pump Flow Rates 0 001 to 5 000 mL min for 5 mL min heads PES isaka rebana nawak 0 to 5 000 psi for SS pump heads 0 to 4 000 psi for PEEK heads Pressure Accuracy 2 of full scale pressure Pressure Zero Offset 2 psi Flow Accuracy s 2 1000psi 1 mL min with 20 IPA Flow Repeatability 0 2 RSD Dimensions 08 6 75 high x 14 wide x 17 5 deep Welah tinas 50 Ib POWER ul sige 110 120 Vac 50 60 Hz 0 5A 45W or 220 240 Vac 50 60 Hz 0 2A 45W Features ui aas Prime purge valve Pulse damper User settable upper and lower pressure limits Outlet filter Autoprime purging Remote Inputs RS 232 1 3 2 INSTALLATION 2 1 Unpacking and Inspection Prior to opening the shipping container inspect it for damage or evidence of mishandling If it has been damaged or mishandled notify the car
43. phire piston is fragile Twisting the pump head during replacement can cause the piston to break Closely follow instructions during head removal and replacement to avoid breakage 5 8 1 Make sure that the inlet valve is on the bottom and the outlet valve is on the top Carefully align the self flush housing and gently slide it into place on the pump If misalignment with the piston occurs gently push up on the piston holder 2 Line up the pump head and carefully slide it into place Be sure that the inlet valve is on the bottom and the outlet valve is on the top Do not force the pump head into place 3 Finger tighten both knurled nuts into place To tighten firmly alternately turn nuts 1 4 turn while gently wiggling the pump head to center it CAUTION Use care when replacing the pump head Twisting the pump head can cause the piston to break 4 Re attach the inlet and outlet lines Reconnect the self flush lines and fittings to the self flush check valves Change the flushing solution 5 3 Conditioning New Seals NOTE Use only organic solvents to break in new seals Buffer solutions and salt solutions should never be used to break in new seals Using a restrictor coil or a suitable column run the pump with a 50 50 solution of isopropanol or methanol and water for 30 minutes at the back pressure and flow rate listed under PHASE 1 below and according to the pump head type Then run the pump for 15 minutes at
44. rfacing 5 sak i ee le ep a eet deen eee eee 4 2 4 2 4 Motor Stall Detecior iii r ee vs She AN ge en dw ee a a etree das oe oD a ad a aa a od RE eos 4 2 MAINTENANCE asik un san aa sala pangan ba a a buak A naas D a a aa 5 1 5 1 Filter Replacement oocimiocinccccconniccc ieniaa renine airian eana ma nanaon aeaee reaa Aaaa eaaa Naa AeA aa EA AE Eana iera iadaaa apie aaia Eaei Ee 5 1 is wih Rect eth a A a a Da ION aa eee ea ede eee 5 1 5 2 Changing PUMP HOAdS ci a a da dae 5 1 9 2 1 Removing E Pump A640 3 02 ae Ae A Ae AS A as 5 1 5 2 2 Cleaning the Pump Head ASSOMDI 1 ccccccecccesceceneteneeceneeceeeceaeeseneeseaeeseaeeseaeeseaeeseaeeseaeeseaeeseeeeseeseesseneetanees 5 4 2 3 Replacing Piston Seals sani sarna NGA ENE eh E NGENEH ni ees edited eles NENGA ten ete 5 6 5 2 4 Changing the Pistoniccc3 dene anal A a ans ded onan 5 8 9 2 5 Replacing the Pump Head esos ease aie ie A ae A a 5 8 5 3 Conditioning New S al S sas ia aeara Naah ANGEN ANNA ek daa 5 9 5 4 Check Valve Cleaning and ReplaCemenit 1scssccsecccceesssenescnnesscenssennssnenssenassnenssenasscanssennsssanesennsseansennsseansseensseanes 5 9 5 5 Pulse Damper Replacement cociccccicccccononicccnccn anae aane anana nana eaaa nagan anganan ena nn eaaa ean anana egan NEG g Neg aNeEnanENe 5 10 5 5 1 Removing the Pulse Damper occ taa a TE E aana 5 10 5 5 2 Pulse Damper ReturbiShing ss ssssas saras rasana tiene 5 11 5 6 3 Pulse Damper Installation ad dais 5 11 5 6 Cleaning the PUMP A
45. rier before opening the container Once the container is opened inspect the contents for damage Any damage should be reported to the carrier immediately Save the shipping container Check the contents against the packing list 2 2 Location Environment The preferred environment for the Q Grad pump is normal laboratory conditions The area should be clean and have a stable temperature and humidity The specific temperature and humidity conditions are 10 to 30 C and 20 to 90 relative humidity The instrument should be located on a stable flat surface with surrounding space for ventilation and the necessary electrical and fluid connections 23 Electrical Connections Unpack the Q Grad pump and check the voltage setting on the Power Entry Module on the back panel see Figure 2 1 Make certain the voltage setting agrees with the power to be supplied to the unit A pump which is connected to a 100 120Vac voltage source should have a voltage setting of 115V and a pump connected to a 220 240Vac voltage source should have a voltage setting of 230V The pump can be configured with either of the two voltage configurations If the voltage setting is correct position the pump so that there is at least a four inch clearance on all sides to permit proper ventilation Then plug the pump into a properly grounded electrical outlet WARNING Do not bypass the safety ground connection as a serious shock hazard could result If the pump do
46. s in the power entry module To gain access to these fuses gently pry off the cover plate with a small flat bladed screwdriver Replace with fuses of the correct rating 1 A slow blo for 115 Vac pumps or 1 2 A slow blo for 230 Vac pumps see Section 2 3 If the fan is running but one or more pump motors do not run check the fuse located on the motor power circuit board that is not running Replace it with a 5 A fast blo fuse 5 9 Battery Replacement The battery provides power for the memory that holds the current pump configuration of Pump A If Pump A is set at a flowrate other than 1 00 or 5 0 and the power is turned off when the power is turned back on the flowrate should appear as it was set If this flowrate does not appear the battery will need replaced CAUTION Circuit boards can be damaged by Electro Static Discharge ESD Follow standard ESD procedures when handling circuit boards 1 Unplug the unit 2 Remove the cover 5 12 Turn the unit so that the pump heads are to the right The battery can be seen in the lower right corner of the Pump A circuit board Remove two screws from the bottom of the cabinet that hold the circuit board set for Pump A Gently angle the loose circuit board to gain access to the battery socket The battery is circular and has a positive pole mark on the top Gently pull it from its socket With the positive mark up gently slide the new battery into the battery socket Be sure th
47. ses must be placed fully to the rearmost end of the tray closest to the metal tabs extending from the tray or contact will not be made and the unit will not operate Both fuses must be present to complete the circuit With the two proper fuses in the tray orient the tray with the two fuses at the sides and the 230V marking at the top as shown in Figure 2 2 and insert it with the metal tabs first into the top of the power entry module until it is firmly seated in place It may be necessary to squeeze the two fuses at the rear to permit them to enter the power module housing Close the power entry module cover by pressing at the top center until it snaps into place Insert a suitable 230V power cord 2 3 2 Converting from 230Vac to 115Vac 1 Remove the power cord from the power entry module see Figure 2 1 located in the rear of the pump Using a small flat blade screwdriver carefully pry out the power entry module cover at point A Figure 2 1 The hinged cover will swing down as shown in Figure 2 2 Using a small flat blade screwdriver carefully pry out at the top of the fuse tray and carefully remove the tray as illustrated in Figure 2 2 Remove the fuses present in the fuse tray by gently lifting them up at the rearmost ends of the tray where the metal tabs extend and removing the fuses from their retaining clips 5 Observe the shorting clip placement as shown in Figure 2 3 Place a shorting clip by hand onto the tray as
48. try module Close the power entry module cover by pressing at the top center until it snaps into place Insert a suitable 115V power cord 2 4 Solvent Preparation Proper solvent preparation will prevent a great number of pumping problems The most common problem is bubble formation which may affect the flow rate consistency Aside from leaky fittings the problem of bubble formation arises from two sources solvent out gassing and cavitation Filtration of HPLC solvents is also required 2 4 1 Solvent Out gassing and Sparging Solvent out gassing occurs because the mobile phase contains dissolved atmospheric gases primarily Nz and O2 These dissolved gases may lead to bubble formation and should be removed by degassing the mobile phase before or during use The best practical technique for degassing is to sparge the solvent with standard laboratory grade 99 9 helium Helium is only sparingly soluble in HPLC solvents so other gases dissolved in the solvent diffuse into the helium bubbles and are swept from the system Solvent filtration is not an effective alternative to helium degassing It is recommended that you sparge the solvent vigorously for 10 to 15 minutes before using it Then maintain a trickle sparge during use to keep atmospheric gases from dissolving back into the mobile phase The sparged solvent must be continually blanketed with helium at 2 to 3 psi Non blanketed sparged solvents will have atmospheric gases dissolved b
49. u look into the pump head cavity only the polymer portion of the seal should be visible 2 Place a self flush replacement seal on the seal insertion removal tool so that the spring in the seal is visible when the seal is on the tool As in the previous step insert the tool and seal into the seal cavity on the flushing housing taking care to line up the seal with the cavity and then withdraw the tool When the seal is fully inserted only the polymer part of the seal will be visible in the seal cavity 3 Place seal back up washer over the high pressure seal Place seal back up plate back into pump housing if it was removed Orientation is not important in these cases 4 Attach the pump head as described in Section 5 2 5 Use caution so as not to damage the sapphire piston 5 Condition the new seal as described in Section 5 3 5 2 4 Changing the Piston 1 Remove the pump head as described in Section 5 2 1 Use caution so as not to damage the sapphire piston 2 Grasp the metal base of the piston assembly so that you avoid exerting any side load on the sapphire rod and remove the piston from the slot in the carrier by sliding it up 3 Grasp the metal base of the replacement piston assembly and insert it into the slot in the piston carrier until it bottoms in the slot 4 Attach the pump head as described in Section 5 2 5 Use caution so as not to damage the sapphire piston 5 2 5 Replacing the Pump Head CAUTION The sap
50. ump head Tighten if necessary Replace seals Replace piston guide and seals See Sections 5 2 and 5 3 Consider changing to a self flushing pump head if using buffers Clean excess lubricant and dirt off piston carrier See Section 5 7 Check to be certain the low pressure limit is set to O psi Only increase the low pressure limit after the pump attains operating pressure Contact service technician Remove and clean both the inlet and bulkhead filters See Section 5 2 If the problem persists remove tubing from system one piece at a time until you find the clogged piece Most clogs occur outside the pump itself Replace only with the appropriate fuses 1A for 100 120 Vac or 1 2A for 220 240 Vac Contact service technician if problem persists Replace only with the appropriate fuse Contact service technician if problem persists Clean and dry mating surfaces If scratched replace defective part Replace O ring Tighten head Replace leaky parts 6 LIST OF REPLACEMENT PARTS Stainless Steel Part No 120679 210140 210142 140199 220299 020294 211152 Bioclean PEEK Seals General Part No 060141 060100 060109 060159 060108 140198 060195 060116 211151 Part No 160559 902280 10 Part No 060110 060111 120682 160565 120886 931617 Description Check valve kit for one pump head 1 16 gland nut for outlet tubing 1 16 ferrule for outlet tubing Pu
51. xx OK Sets the flowrate to xx xx mL min where the range is fixed for the pump head size i e for 0 01 to 10 00 mL min xxxx 0001 to 1000 OK x xxx mL min for 0 001 to 5 000mL min xxxx 0001 to 5000 OK x Reads the pump s current pressure where X XX XXX OF XXXX X XX XXX Or xxxx Current pressure in PSI Valid only for pump A OK x y yy Reads the pump s current pressure and flowrate where X XX XXX Or xxxx Current pressure in PSI X XX XXX OF XXXX y yy or yy yy Flowrate in mL min y yy or yy yy CS OK x xxx y z PSI w v u Reads the current pump setup where X XXX or xx xx Flowrate in mL min X XXX OF XX XX y yy yyy or yyyy Upper pressure limit Y yy yyy Or yyyy Z ZZ ZZZ Of ZZZzZ Lower pressure limit Z ZZ ZZZ Of ZZZZ PSI Units PSI ATM MPA BAR or KGC w Pump head size 0 standard v Run status 0 stopped 1 running u Pressure Board present 0 otherwise 1 OK vx xx SR30 firmware Identifies the pump type and EPROM revision x xx UPxxxx OK Sets the upper pressure limit in PSI The maximum value for xxxx is 5000 for the plastic heads or 6000 for the steel heads the minimum value is the lower limit plus 100 The value must be expressed as four digits i e for 900 PSI xxxx 0900 Valid only for pump A 3 4 LPxxxx OK Sets the lower pressure limit in PSI The maximum value for xxxx is the current upper pressure limit setting minus 100 the minimum value
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