SC-20 User`s Manual (Rev. 030918.1)
Contents
1. Accuracy Perfusion lines Internal Dead Volume Water Jacket Ports Controller Physical Dimensions Cable Length Connector Type Weight Warranty 0 C 2 ml min max flow 50 C 5 ml min 0 1 C Type 316 Stainless Steel 0 032 ID x 0 062 OD 330 ul Type 316 Stainless Steel 0 12 ID x 0 147 OD Model CL 100 Bipolar Controller 21 x 165 mm dia x length 1 9 m 15 pin Male D 109g 1 year A 7 Warner Instruments Warranty and service Warranty The model SC 20 is covered by our Warranty to be free from defects in materials and workmanship for a period of one year from the date of shipment If a failure occurs within this period we will either repair or replace the faulty component s This warranty does not cover instrument failure or damage caused by physical abuse lack of maintenance or electrical stress inputs exceeding specified limits In the event that instrument repairs are necessary shipping charges to the factory are the customer s responsibility Return charges will be paid by Warner Instruments Normal business hours are 8 30 AM to 5 30 PM EST Monday through Thursday and 8 30 AM to 5 00 PM on Friday Our offices are located at 1125 Dixwell Avenue Hamden CT 06514 and we can be reached by phone at 800 599 4203 or 203 776 0664 Our fax number is 203 776 1278 In addition we can be reached by e mail at support warneronline com or through the web at hitp www warnero2. A 1 Warner Instruments The lower perfusion line in the picture enters into the PHC Series imaging chamber heater cooler jacket This line carries heated chilled water comes from a circulating reservoir and is used to aid to maintain the temperature of the chamber platform Tubing Connections Solution flow lines PE 160 tubing or any other tubing with a 1 16 1 5 mm ID is used to connect to both of the available input and output solution ports on the SC 20 Connections are made by press fitting the tubing onto the 18 gauge stainless steel hypodermic tubes Cap plugs are supplied to block unused inputs Water Jacket flow line Water is circulated through the SC 20 WATER JACKET via the two large ports located on the input side of the heater cooler Run Tygon flow lines in a loop from your water source a sink or water bath to both ports the SC 20 Connections are made to the SC 20 by press fitting the tubing onto the large bore stainless steel tubes Use of the INTEGRAL WATER JACKET to remove excess heat from the internal Peltier device is critical to the function of the SC 20 and its use is mandatory PHC Series Imaging Chamber Heater Cooler Jacket The use of a separate water jacket attached to a Series 20 chamber to aid in maintaining the temperature of the chamber represents an improvement in function for systems using an upright microscope PHC Series water jacket water is passed through the SC 20 via one of the solution flow pa
3. and simple to use thermal control device In line solution heating has proven to be one of the most effective methods of maintaining the temperature of perfusion solutions The SC 20 uses reliable and precise Peltier technology to regulate one or two solutions above or below ambient levels Solution temperatures can be maintained at O C at flow rates of 2 ml min at 5 C at 5 ml min or as high as 50 C at 5ml min An integral water jacket using running water from a tap or large reservoir is used to remove excess heat from the Peltier electronics within the SC 20 Flow rates within the water jacket as low as 4 liters per hour are sufficient to maintain thermal efficiency of the device The SC 20 can be used to control one or two discrete perfusate solutions or a solution gas combination When coupled with a PHC Series imaging chamber heater cooler jacket the SC 20 provides an effective means for temperature control in a Series 20 imaging and recording chamber even in the absence of solution flow A 7 Warner Instruments GENERAL In line solution heating is the simplest and most effective method of warming or cooling perfusion solutions prior to use The minimal dead space manifold at the heater cooler output allows it to be used in applications where from 1 to 2 perfusion lines are connected to a chamber or other device The SC 20 Dual In Line Solution Heater Cooler is designed for use with the Warner CL 100 Bipolar Temperature Control
4. SC 20 Manual Rev 030918 1 Warner Instruments Dual In Line Heater Cooler Model SC 20 Warner Instruments 1125 Dixwell Avenue Hamden CT 06514 800 599 4203 203 776 0664 203 776 1278 fax AZ Warner Instruments SC 20 Manual Rev 030918 1 Table of Contents INTRODUCTION cC HO 4 LOTO HL c 4 SE DUD mc C 4 Tubins COMME CH ONS D 5 SOLO fIOW INES C 5 Water Jacket flow line esteso ee eim eese t rine se ss SEE eee REP R ERR Pes 5 PHC Series Imaging Chamber Heater Cooler Jacket cec ee ee ecce e etes eee ess essere 5 Component Placement Pe EC o 5 OPERATION 6 USING WITH A CHAMBER sessssssscssccicisecesscscesoscctscessssexessecscesecsetvorectctsavssseestscsbetvers ne Ska ao oe cdesssetelescstesans 6 OUTGASSENG 6 APPENDIX qe 7 WAU LO TANICE eot 7 SDCCHICALIONS ERE EET ILLE LLL LIUC 7 Warranty and a T e M 8 Zona 6 DOTVIGE NOLES ses aka p a ta A 7 Warner Instruments The SC 20 Dual In Line Solution Heater Cooler from Warner Instruments is a versatile
5. ler The compact design of the SC 20 makes it possible to install the device immediately adjacent to the input port of a perfusion chamber insuring minimal heat loss The SC 20 will easily accommodate flow rates up to 5 ml min Each heater cooler is supplied with a TA 29 thermistor cable assembly which allows for monitoring of the actual bath temperature and 3 meters each of PE 160 and 4 in OD Tygon tubing SETUP A general configuration for the SC 20 is shown below Note that the illustration here makes use of a PHC Series imaging chamber heater cooler jacket to provide additional thermal control for the imaging and recording chamber n WARNER LOU The SC 20 has two input ports and two corresponding output ports for fluid flow to the sample chamber These lines are attached to the INPUT and OUTPUT PERFUSION PORTS and are identified above The two WATER JACKET INPUT OUTPUT PORTS are the larger diameter ports on the input side of the SC 20 and are also identified The WATER JACKET PORTS are provided for connecting to the INTEGRAL WATER JACKET within the SC 20 If you look closely at the routing of the two perfusion flow lines exiting the SC 20 the two lines enter into the Series 20 chamber and platform on the right hand side of the picture you will see that the upper perfusion line enters into the chamber s input port This line carries your experimental perfusion solution and would come from your solution reservoir
6. nline com Service notes Please refer all questions regarding service to our Engineering Department A Should service be required please contact the factory The problem may often be corrected by our shipping a replacement part Factory service if required will be expedited to minimize the customer inconvenience B Instruments are inspected immediately upon receipt and the customer is notified if the repair is not covered by the warranty Repairs can often be completed in 1 2 days from our receipt of the instrument C If factory service is required please observe the following instructions 1 Package the instrument with at least 3 inches of cushioning on all sides Use the original shipping carton if it is available 2 Insure the shipment for its full value 3 Include with the shipment an explanation of the problem experienced A 7 Warner Instruments
7. stor into the bath to measure the temperature at the point of interest Adjust the SET TEMP to the desired operating temperature Allow the system to thermally stabilize Monitor the temperature at the point of interest using the TA 29 thermistor Make any temperature adjustments on the CL 100 to compensate for heat loss from the SC 20 to the bath Additional adjustment of the heater set point may be required if any of the following change substantially during an experiment e Solution flow rate e Temperature of solution entering the heater e Ambient room temperature e Air currents around chamber Efforts to minimize these factors will be rewarded OUTGASSING A common problem with rapid heating of solutions is outgassing The bubbles formed can often cause blockages or disruptions to the flow in the chamber bath An effective solution to this problem is to pre warm the perfusate at the reservoir Warner Instruments carries a full line of Solution Reservoir Heaters designed to work in concert with our In Line Solution Heaters A 7 Warner Instruments APPENDIX Maintenance Salt solutions can be corrosive to metal components and can shorten the life of the heater cooler if left in the unit during storage The SC 20 should be flushed with distilled water and blown dry after each use to eliminate the effects of salt and moisture Specifications Minimum Temperature Maximum Temperature Maximum Flow Rate at 5 C
8. ths and is channeled to the PHC Series water jacket lying under the chamber Since both the perfusion solution and PHC Series water jacket lines are routed through the same heater cooler their temperature will be maintained to the same value Component Placement 1 Plug the SC 20 into the rear of the CL 100 Bipolar Temperature Controller using the attached cable 2 Attach solution flow lines from the solution reservoir s to the input ports of the SC 20 3 Attach the SC 20 INTEGRAL WATER JACKET flow lines to the SC 20 WATER JACKET PORTS 4 Using a support stand place the SC 20 in a convenient location usually adjacent to the sample chamber 5 Run solution flow lines from the output ports of the SC 20 to the input ports of the perfusion chamber 6 The supplied TA 29 thermistor assembly is used to monitor the temperature at a point of interest Plug the TA 29 into the MONITOR TEMP IN BNC on the CL 100 and place the thermistor at a point of interest A 7 Warner Instruments OPERATION USING WITH A CHAMBER l 2 Turn power to the CL 100 on Set the CL 100 MODE SELECTOR SWITCH into auto the INTERNAL EXTERNAL SELECTOR SWITCH to internal see the CL 100 user s manual and the LOOP SPEED SELECTOR SWITCH to medium Start the solution flow to the SC 20 INTEGRAL WATER JACKET Start solution flow through the SC 20 into the chamber and to the PHC Series Imaging Chamber Heater Cooler Jacket if used Place the TA 29 thermi
Download Pdf Manuals
Related Search