LIH 8+8 Manual - HEKA Elektronik Dr. Schulze GmbH
Contents
1. Important note In a Windows system if sound is enabled a beep will be heard when the operating system has loaded unloaded the USB driver Once the USB cable is disconnected wait to hear this beep before re connecting the cable otherwise the driver may not load correctly 3 Driver Errors The LIH 8 8 identifies itself to the host operating system without the need of any additional driver files However for http www heka com 25 the application software to communicate with the LIH 8 8 the files LIH88Driver dll and LIH88Service exe are required These files are provided and installed by the application program itself If driver errors are displayed while running the application software verify that these files exist in the applications working directory Data Throughput Issues Data throughput problems can be caused by any number of things including CPU speed hard disk speed available RAM etc The following can be easily verified a Check that the LIH 8 8 is connected to a USB 2 0 Hi Speed port as outlined above and that the port is not shared by any other device USB specifications allows up to 127 devices to be connected on a single USB port This is very reasonable for low bandwidth devices but not recommended for high speed devices like the LIH 8 8 data acquisition system b Check that the acquisition program has enough RAM available During Acquisition it is advisable to disable any software appli cat2. AD 0 AD 1 and AD 2 AD 3 The 5 us time delay is shown in the insert The four 16 bit D A converters feature high accuracy with low noise This low noise allows for the observation of least significant bit transitions The D A converters update simultaneously http www heka com 1 5 Hardware Description 7 1 5 5 Differential Inputs The LIH 8 8 was designed with differential A D inputs to provide the most accurate measurements Differential inputs are isolated inputs since they are referenced to a ground point that is not connected to earth ground Therefore differential inputs eliminate common mode voltage errors that can occur and are required for measuring low level signals Other data acquisition interfaces that use single ended analog inputs are non isolated inputs that are referenced to earth ground Non isolated sys tems also connect the digital I O signals to the same ground If the record ing setup shares the same ground as the data acquisition interface typically via the building s AC power the difference in ground potential between the two devices can be substantial Fast switching currents must then travel between the various devices of the recording setup to the computer De pending on how single ended inputs are connected ground loop errors can be introduced resulting in inaccurate measurements 1 5 6 FIFO Memory The LIH 8 8 is supplied with two 512 kilosample FIFOs one each for input and output data poin
3. 8 IN 11 9 IN 4 10 IN 12 11 IN 5 12 IN 13 13 IN 6 14 IN 14 15 IN 7 16 IN 9 17 IN 1 18 NO CONNECTION 19 GND 20 GND 21 GND 22 GND 23 NO CONNECTION 24 STROBE 25 OUT 0 26 OUT 8 27 OUT 1 28 OUT 9 29 OUT 2 30 OUT 10 31 OUT 3 32 OUT 11 33 OUT 4 34 OUT 12 35 OUT 5 36 OUT 13 37 OUT 6 38 IN 8 39 OUT 7 40 NO CONNECTION http www heka com 7 2 DIGITAL IN Connector 41 7 2 DIGITAL IN Connector This is a DB 25 female connector that can be used to interface with the Digital Input lines of the LIH 8 8 13 1 25 14 Figure 7 2 Digital IN Connector Pin number Signal Pin number Signal 1 IN 3 14 IN 4 2 IN 2 15 IN 5 3 GND 16 IN 6 4 GND 17 IN 7 5 GND 18 IN 8 6 GND 19 IN 9 7 GND 20 IN 10 8 GND 21 IN 11 9 GND 22 IN 12 10 GND 23 IN 13 11 GND 24 IN 14 12 IN 1 25 IN 15 13 IN 0 http www heka com 42 Appendix I Technical Data 7 3 DIGITAL OUT Connector This is a DB 25 female connector that can be used to interface with the Digital Output lines of the LIH 8 8 13 25 14 Figure 7 3 Digital OUT Connector Pin number Signal Pin number Signal 1 OUT 3 14 OUT 4 2 OUT 2 15 OUT 5 3 GND 16 OUT 6 4 GND 17 OUT 7 5 GND 18 OUT 8 6 GND 19 OUT 9 7 GND 20 OUT 10 8 GND 21 OUT 11 9 GND 22 OUT 12 10 GND 23 OUT 13 11 GND 24 OUT 14 12 OUT 1 25 OUT 15 13 OUT 0
4. a repair quote will be supplied for approval Repairs are warranted for 90 days 6 5 Technical Support HEKA provides exceptional pre and post sales customer support from our trained international sales representatives Customer support is free of charge for registered users of HEKA products Check the HEKA website www heka com for updated versions of driver and or application software There are also many tutorial documents avail http www heka com 38 Warranty and Support able that may help resolve product specific questions If the problem is not resolved please supply the following information when corresponding e Serial number of the LIH 8 8 interface e Operating system version e Driver software version number Application software version number Detailed information describing the problem http www heka com 7 Appendix I Technical Data 7 1 DIGITAL I O Connector This is a 40 pin male connector that combines the digital input and output lines This connector is to be used to interface to other HEKA devices such as the EPC 8 or the TIB 14 For other purposes the DIGITAL IN and DIGITAL OUT Connectors should be used 40 Appendix I Technical Data PIN 40 PIN 2 Figure 7 1 Digital I O Connector Pin number Signal Pin number Signal 1 IN 0 2 OUT 14 3 NO CONNECTION 4 OUT 15 5 IN 2 6 IN 10 7 IN 3
5. be displayed in the Notebook Window The drag box dialog will also update the appropriate check boxes to match 5 Notebook 07 Sep 2007 07 Sep 2007 17 33 30 digital out 0 0000000000000000 digital out 1024 0000010000000000 digital out 17408 0100010000000000 v The simplest method to verify the functionality of the Digi http www heka com 33 vi tal Outputs is to first enable the Single Bit control Then use the check boxes to toggle the state of each output while monitoring the output on the measuring device Important note A reading above 2 4 Volts is usually considered TTL HIGH while a reading be low 0 5 Volts is TTL LOW Repeat for all Digital Outputs If any of the Digital Outputs fail the test please contact HEKA technical support for assistance j Checking the Digital Inputs A device that can output TTL HIGH 5 Volts is required to test the functionality of the Digital Inputs The LIH 8 8 itself can provide this output either with one of the D A or Digital Output channels b ii lil Assuming that a Digital Output is to be used Connect the Digital Output directly to the Digital Input to be tested Important note Digital Inputs O to 2 are avail able on BNC connectors on the front panel and can be easily accessed All of the Digital Inputs are avail able on a DB 25 connector on the rear panel labeled DIGITAL IN To access these channels a break out cable will have
6. note The procedures outlined below are for testing the stand alone version of LIH 8 8 They are not to be used for testing the functionality of an LIH 8 8 built into an EPC 10 USB Patch Clamp Amplifier Those test procedures are outside the scope of this manual d Before selecting Continue verify that the check box Activate AD DA board is enabled Otherwise the LIH 84 8 interface will not be initialized and the software will execute in Demo mode e Select Continue The software will now proceed with software and hardware initialization If the software applications Main control window is displayed this is a positive sign that the LIH 8 8 has been properly detected and initialized Proper initialization can be visually confirmed by verifying that the Status LED on the front panel is illuminated 34 EpcMaster EB File Edt Windows Notebook Macros EPC7 Help 24 no file ES 16 55 39 Notebook 07 Sep 2007 Bable NOT loaded IGainTable_EPC7 Welcome to EpcMaster v2 21 27 Feb 2007 If the following error window is displayed http www heka com 29 LIH 8 8 error LIH88Driver dll not found v0 0 The sound driver is not properly installed Select retry to ini tialize the driver once more or abort to terminate the sound driver initialization If abort is selected then LIH 84 8 initial ization will continue since this is not considered a fatal error If any of the following
7. of Figures 11 4 1 4 2 7 1 7 2 7 3 Schematic illustrating 100 us data acquisition of two pairs of input channels AD 0 AD 1 and AD 2 AD 3 The 5 us time delay is shown in the insert LIH 8 8 Front Panel Controls LIH 8 8 Rear Panel Controls Digital I O Connector o oo Digital IN Connector o e Digital OUT Connector o o 1 Introduction 1 1 Product Intended Usage The HEKA LIH 8 8 is intended for research use only in a laboratory by persons trained in its use Users are expected to be able to properly operate the LIH 8 8 and associated instruments The HEKA LIH 8 8 is not intended for medical use The HEKA LIH 8 8 is not intended for use in life support situations or in situations were improper operation or failure of the LIH 8 8 can result in personal injury HEKA makes no representation that the design implementation testing or manufacture of the LIH 8 8 meet reasonable standards for use as a medical product As stated in the HEKA Limited Warranty Statement supplied with each product HEKA expressly disclaims all warranties to buyer except the limited warranty set forth above including without limitation to any and all implied warranties of merchantability and fitness for a particular purpose 1 2 Supplied Components The following items should have been packed with your LIH 84 8 Data Acquisition
8. to be fabricated Please refer to Appendiz I of this manual for a complete pin out description Toggle the state of the Digital Output For proper testing verify that the input changes correctly for both HIGH and LOW settings Monitor the Digital Input state by viewing the check boxes next to the text label DIG IN A quick reminder an X within the check box represents a TTL HIGH while a hollow box represents a TTL LOW Dig In DDBDOOOUDODEDODED 9012345 Dig 0utoo00000000800080 CD DSinge Bt iv Repeat the steps outlined above for each Digital Input chan nel If any of the Digital Inputs fail the test please contact HEKA technical support for assistance http www heka com 34 Troubleshooting 6 Noisy A D channel Input Signal Most noise problems are usu ally caused by bad cabling or by other external devices To resolve these problems please try the following a Verify that the LIH 84 8 and the host computer are plugged into a common outlet strip This will minimize power line induced noise sixty fifty hertz signals in the acquired data b Verify that the AC power is clean and that the outlets are prop erly grounded It is strongly advised that this task be handled by a licensed professional c Verify that the BNC interconnect cables used are of high quality 75 Q cables and that both the signal and ground connections are continuous This can be easily checked with either an ohm meter DMM or a cont
9. 0 Benefits e 1 5 8 Expandability 1 5 9 ToneGenerator 22e 1 5 10 Software Support E o d JJ N N OD Ct A FP V V N N N N ii CONTENTS 2 Technical Specifications 11 2 T Analog Inputs amp a4 amp ooh baa ewe wem EGER A 11 2 2 Digitallmputs 4504 da a RP 11 2 9 Trigger Input 1 A a ER o papa AAA de a a Oy 12 2 4 Analog OQUP ee 12 25 Digital Outputs v oa e erts 12 2 6 Tone Generator 13 2 7 Additional Connectors llle 13 2 8 DIMENSIONS x22 3E a EUR ROUX O A 13 2 9 Power Requirements o 13 210 Gables 3 a AA al Ee a Ci do A 13 2 11 Host Interlace a o pr pda de eer A EE A 14 2 12 Warranty 2 2 2 2 Dd A we a A E e el 14 3 Installation 15 3 1 Connecting the LIH sing 15 4 Controls and Functions 17 41 Front panel Controls a 17 4 2 Rear panel controls o o 20 5 Troubleshooting 23 6 Warranty and Support 35 DL Warranty A sane py ae o ad tel EE d s 35 6 2 Limitation of Liability 36 6 3 Obtaining Warranty Service 37 http www heka com CONTENTS iii 6 4 Obtaining Out of Warranty Service o 37 6 5 Technical Support o o 37 7 Appendix I Technical Data 39 7 1 DIGITAL I O Connector sess 39 7 2 DIGITAL IN Connector 41 7 3 DIGITAL OUT Connector eene 42 http www heka com List
10. BNC on front panel 16 bit 1 in 65536 lt 1 us 10 24 to 10 239 Volts 0 2 of FSR lt 2 dB 4 ppm C 4 70 116 dB indefinite 20 mA maximum 2 5 Digital Outputs Number of channels Input type Input connectors Power on state Maximum output current 16 isolated TTL logic compatible 4 on front panel BNC all on rear panel DB 25 connector logic low 35 mA http www heka com 2 6 Tone Generator 13 2 6 Tone Generator Frequency range between 200 Hz and 4 kHz Output connector 3 5 mm audio jack on rear panel 2 7 Additional Connectors USB USB 2 0 female B connector on rear panel Digital IO 40 pin male connector on rear panel for interfacing with EPC 8 or TIB 14 Master sync RJ 45 CAT5 connector on rear panel Slave sync RJ 45 CAT5 connector on rear panel 2 8 Dimensions Width 47 5 cm 19 inches 19 inch rack mountable Height 4 375 cm 1 75 inches Depth 31 1 cm 12 5 inches Weight 3 6 kg 8 pounds 2 9 Power Requirements Input voltage 90 VAC 250 VAC Input frequency 50 60 Hz Maximum power 30 watts 2 10 Cables USB 3 meter USB 2 0 shielded cable Power cord 2 meter IEC type supplied with either a USA UK AU or EU version http www heka com 14 Technical Specifications 2 11 Host Interface USB 2 0 Hi Speed 480 Mbits second 2 12 Warranty Duration One year parts and labor http www heka com 3 Installation 3 1 Conne
11. ED will be illuminated when the LIH 848 has been properly initialized and ready for use External Trigger Input A BNC connector provides access to the Trigger input circuitry of the LIH 8 8 A TTL trigger pulse can be given to start data acquisition The LIH 8 8 can be triggered with either a positive or negative logic pulse depending on software settings with a minimum duration of 30 ns This input is isolated from the computer Important note The absolute maximum voltage range for this input is 0 5 to 5 5 Volts Do not exceed this range or damage can result to the input circuitry Digital Inputs Three BNC connectors provide access to three of the sixteen Digital Input channels These Digital Inputs can be read by the acquisition software These Digital Input channels accept standard TTL level inputs 5 Volts and 0 Volts These channels are isolated from the computer For optimal results use only high quality 75 Q BNC cables Important note The absolute maximum input voltage range s 0 5 to 5 5 Volts Do not exceed this range or damage can result to the input circuitry http www heka com 4 1 Front panel Controls 19 6 Digital Outputs Four BNC connectors provide access to four of the sixteen Digital Output channels The acquisition software can control the state of the Digital Output channels These Digital channels output standard TTL levels 5 Volts and 0 Volts These channels are isolated from the com
12. Manual 1 0 e dur um 8 8 Data Acquisition Interface O 19191 9 9 HECA HEKA Elektronik Dr Schulze GmbH Wiesenstrae 71 D 67466 Lambrecht Pfalz Germany HEKA Electronics Inc 47 Keddy Bridge Road R R 2 Mahone Bay NS BOJ 2E0 Canada HEKA Instruments Inc 20E Vanderventer Ave Suite 102 Port Washington NY 11050 USA Phone Fax Web Site Email Phone Fax Web Site Email Phone Fax Web Site Email Title Page LIH 8 8 Acquisition Interface 49 0 6325 95 53 0 49 0 6325 95 53 50 http www heka com salesQheka com support heka com 1 902 624 0606 1 902 624 0310 http www heka com nasales heka com supportGheka com 1 516 882 1155 1 516 467 3125 http www heka com ussalesQheka com supportQheka com 2007 HEKA Elektronik Dr Schulze GmbH COMLMA 1 Contents 1 Introduction 11 1 2 1 3 1 4 1 5 Product Intended Usage o Supplied Components less 1 2 1 For a single rack unit o 1 2 2 Fora dual rack unit e Unpacking gt ua as deu eR rue dp rere pu s Computer Requirements 14 1 Macintosh mesa A ee eon a 1 2 Windows dee a al esee nope Aen Hardware Description 1 5 1 Main Features 1 5 2 Hardware Configuration 1 5 3 selber 54 dose aoe eh Oe A Hob e A e e 1 5 4 Analog Circuitry e 1 5 5 Differential Inputs 1 5 6 FIFO Memory e 15 7 USB 2
13. System 1 2 1 For a single rack unit e 1 LIH 8 8 data acquisition interface e 1 USB 2 0 shielded cable 3 meters e 1 HEKA CD ROM 2 Introduction e 1 Printed user s manual e 1 power cord 110 or 220 Volt depending on application 1 2 2 For a dual rack unit e 2 LIH 84 8 data acquisition interface e 2 USB 2 0 shielded cable 3 meters e 1 HEKA CD ROM e 1 Printed user s manual e 2 power cord 110 or 220 Volt depending on application If any of these items are missing please contact HEKA or your dealer im mediately 1 3 Unpacking After unpacking the LIH 8 8 and accessories from the shipping carton please inspect each piece for any signs of shipping damage Please contact the delivering carrier and HEKA immediately if there is any damage Do not dispose of the shipping carton The carrier will want to examine the shipping carton to process a damage claim HEKA insures all shipments to cover shipping damage It is also advisable to keep the shipping carton in the event that the instrument must be returned for service 1 4 Computer Requirements 1 4 1 Macintosh e Macintosh G5 or faster processor e Mac OS X 10 39 or higher http www heka com 1 5 Hardware Description 3 e Available USB 2 0 Hi Speed port 480 Mbits second e CD ROM or internet access 1 4 2 Windows e Pentium 4 or faster processor e Windows 2000 XP or Vista e Available USB 2 0 Hi Speed port 480 Mbits second e CD ROM or internet a
14. abricated Please refer to Appendix I of this manual for a complete pin out description Important note It may be tempting to insert the tip of either the oscilloscope probe or the DMM directly into the DB 25 connector but it is not rec ommended Damage to both the probe and connector can occur Hein EET SEER PERE Dig Outooooooo0000m8000a80 Cea 17498 Single Bit ii First it is always best to start the test procedure by setting all of the Digital Outputs to a known state The Clear button provides an easy way to set all of the Digital Outputs to OFF TTL LOW iii There are a couple of ways to control the status of the Dig ital Outputs The check box item Single Bit provides a way to set either one or multiple Digital Outputs at a time If enabled only one output channel at a time can be active The others will be automatically cleared If disabled the state of multiple channels can be changed iv The Digital Output state can be set either by toggling the individual check boxes or by numerical entry via the Drag box dialog The Drag box dialog uses a numerical value to represent the Digital Outputs as a 16 bit word This dialog is also affected by the setting of the Single Bit control If enabled the software will automatically convert the entered numerical value to the closest value that represents a single bit transition If disabled then multiple channels bits will be set With either entry method the bit values will
15. andard 3 5 mm audio connector is provided for connection to an amplified speaker or headset The tone generator has a frequency range between 200 Hz and 4 kHz which is controlled by software http www heka com 5 Troubleshooting Most problems arise from incorrect installation of the hardware and or incorrect use of the software When troubleshooting it is best to simplify both software and hardware configurations as to remove as many unknowns as possible This chapter will address some of the most common problems e Make sure that no other programs are actively running including virus checking and internet access software e Disconnect ALL external instruments and test the LIH 8 8 com puter combination by itself e If possible swap in a known working LIH 8 8 as a quick way to determine if the problem is the LIH 8 8 or the computer e Make sure that the latest versions of the application software are installed 1 Power Switch not Iluminated a First check that the power switch located on the rear panel is toggled to the ON position b Check to see that the AC line cord is plugged in correctly If attached to a power strip check that the power strip switch is in the ON position and the circuit breaker has not tripped c Check the fuse located on the rear panel The fuse can be easily removed by using a flat blade screwdriver If the fuse needs to be replaced please use the appropriate fuse type as listed i
16. angle and click with the mouse button Simple rectangles without drop shadow A text label within a rectangle is used to display a measured value http www heka com 27 This dialog is for display only and cannot be changed by the user BlSingle Bit Check box A square box with or without a text label on the outside of it behaves like a toggle switch To toggle the switch position the mouse pointer on the center of the check box and click with the mouse button An X within the check box D represents an ON condition while a hollow box O represents OFF Start the software application If the default configuration was saved in a previous session then hardware and software initial ization will begin immediately Otherwise the following message window will be displayed Default CONFIGURATION File not found a Find Fle Select Set Defaults The hardware selection window will be displayed Select Amplifier or AD DA board to use EPC 10 Plus EPC 10 USB Boards Auto Detect EPC 10 OEPC9 O EPC 8 remote mode O ITC 16 O EPC 8 local mode O ITC 18 EPC 7 and others O LIH 1600 DI Activate AD DA board REIH 8 8 Select the option EPC 7 and others and the interface LIH 8 8 Since these test procedures are geared for testing the LIH 8 8 the amplifier selection of EPC 7 and others is used to basically specify no amplifier http www heka com 28 Troubleshooting Important
17. aster shipping service is desired by the customer any additional special delivery expenses must be paid by the customer This warranty does not apply to shipping damage HEKA fully insures all shipments Any claims of damage upon receipt must be filed with the carrier and HEKA immediately This warranty does not apply to any exterior appearance item of the Prod uct which has been damaged or defaced which has been subjected to mis use and abuse abnormal service or handling or which has been altered or modified in design or construction This warranty does not apply to any interconnection cables supplied with the Product This warranty does not apply if any unauthorized repairs modifications or alterations have been made to the Product without written approval from HEKA This warranty applies to software products only to the extent of mainte nance release software to correct improper operation of the Product Soft 36 Warranty and Support ware updates to increase the capabilities of the present Product are not to be provided under the terms of this warranty Updates will be available for free download on www heka com No sales organizations other than HEKA itself are authorized to make any warranties other than those described above or to extend the duration of any warranties beyond the time period described above on the behalf of HEKA If HEKA agrees to such a modification of this warranty HEKA will furnish a modified
18. ccess Please note that many newer computer systems are supplied with multi ple USB 2 0 ports but only some of these ports are capable of Hi Speed operation The LIH 8 8 will work on the slower speed ports but the maximum transfer rates will be greatly reduced For optimal performance the LIH 84 8 should only be connected to a USB 2 0 Hi Speed port If your computer system does not have a Hi Speed USB 2 0 port then a USB 2 0 adapter may be used These adapters are available in many configurations PCI PC Slot etc 1 5 Hardware Description The LIH 8 8 is a high resolution low noise scientific data acquisition sys tem It was specifically designed for electrophysiological applications but it s versatility allows it to be used for any general purpose data acquisition need The LIH 8 8 utilizes the latest Analog to Digital Digital to Analog USB 2 0 and high speed processor technologies to provide expandability and versatility for all current and future requirements With it s USB 2 0 Hi Speed interface the LIH 84 8 is truly a plug and play device that can be easily migrated from various computer systems and measuring setups http www heka com Introduction 1 5 1 Main Features Expandable fully synchronized data acquisition system All synchronous analog and digital channels are isolated Eight differential analog input channels Two 16 bit 200 kHz A D converters multiplexed into two banks of four input channels Si
19. ce in use it and unplug it when done There is no need to power down the computer The LIH 8 8 is selfenumerating and self identifying The device driver is dynamically loaded when connected and dynamically unloaded when unplugged 1 5 8 Expandability If an application requires more channels than are available on a single LIH 8 8 then multiple units can be connected and fully synchronized Each LIH 8 8 unit requires a dedicated Hi Speed USB 2 0 port and an external cable connection between the units This external connection is used to synchronize the acquisition clocks of the units and is made with a standard CAT5 patch cable Note that LIH 8 8 interfaces installed on separate computers use a single external trigger to start the multiple units simultaneously Important note The hardware design of the LIH 84 8 will allow any number of units to be installed only limited by the number of available Hi Speed USB 2 0 ports Currently there is a software limitation which only allows one master slave com bination basically doubling all of the input and output channels http www heka com 1 5 Hardware Description 9 1 5 9 Tone Generator The LIH 84 8 has a built in tone generator that is controlled by software The tone generator has a frequency range between 200 Hz and 4 kHz A 3 5 mm audio connector is provided on the rear panel for connection to an amplified speaker or headset One of the features of the PATCHMASTER software
20. copy of this agreement which must be presented if a claim is being made under these modified terms 6 2 Limitation of Liability Title risk of loss or damage and other incidents of ownership shall pass to the customer upon due tender of goods HEKA shall not be liable for delays in delivery or failure to perform due directly or indirectly to i causes beyond HEKAs reasonable control ii acts of God acts including failure to act of any governmental authority de jure or de facto wars declared or undeclared governmental priori ties port congestion riots terrorism revolutions fires floods sabotage nuclear incidents earthquakes storms epidemics or iii inabilities due to causes beyond HEKA s reasonable control to obtain necessary labor ma terials components facilities energy fuel transportation governmental authorizations or instructions material or information required from the customer Not withstanding any other provisions the total limitations of HEKA including its subcontractors or suppliers on any and all claims whether in contract warranty or otherwise arising out of connected with or resulting from the performance or non performance of any agreement resulting here from or from the manufacture sale delivery resale repair replacement or use of any equipment or the furnishing of any service shall not exceed the price allocable to the Product or service which gives rise to the claim Any such
21. cting the LIH 848 1 The LIH 8 8 can be installed into a standard nineteen inch instru ment rack or used as a desktop unit If installing on a rack please do not use the LIH 8 8 as a shelf to support any other instrument The LIH 8 8 case was not designed to do this and damage to the front panel will result To minimize noise it is advisable to mount the LIH 8 8 away from devices that emit high frequency signals i e monitors power supplies etc 2 Connect the power cord to the LIH 8 8 The internal power supply used in the LIH 8 8 is an auto switching multi voltage supply that will operate from 90 Volts to 250 Volts Make sure that the LIH 8 8 power cord is plugged into a properly grounded AC receptacle Im proper grounding of the LIH 8 8 could result in an electrical shock hazard It is advisable to plug all equipment into a common outlet strip This will minimize power line induced noise in the system 3 Install the USB cable from the USB connector on the rear panel of the LIH 8 8 labeled USB to an available USB 2 0 Hi Speed port on the computer This connection should be made directly to the computers USB 2 0 port and not to a USB HUB 4 As soon as the LIH 8 8 is detected by the host operating system the appropriate system files will be initialized and the LIH 8 8 will be ready for use Important note The host operating system treats the LIH 8 8 as it would any Flash memory device There fore only standard operating system f
22. ctly to the mea suring device using the appropriate BNC cable ii The D A channel voltage can be set by using the appropri ate drag box dialog located under the label DA Channels D A Channel 0 is the leftmost drag box The other channels follow in sequence from left to right DA Channels 101 0 000 V 0 000 V 0 000 V Once a new value has been entered the output voltage on the D A will be changed The D A channel number and value will also be displayed in the Notebook Window 5 Notebook 07 Sep 2007 DA O 10 000 V ii If the value displayed on the measuring device is similar to the specified value the D A channel is working cor rectly For proper testing output voltages of 10 5 0 5 and 10 Volts should be verified for each output channel Important note A minor difference between the set and measured value is normal If the difference is more than a few millivolts please contact HEKA technical support for assistance iv Repeat the steps outlined above for all remaining D A chan nels http www heka com 31 h Checking the A D Inputs The most accurate way to verify the operation of the A D channels is with a calibrated voltage source If the D A channels of the LIH 8 8 have been verified they can be used n i Assuming that a D A output to be used has been verified refer to instructions above for reference connect the D A to the A D channel to be tested Set the D A output v
23. e and installing the proper data acquisition software 1 5 3 Isolation All of the analog input and output channels of the LIH 8 8 are isolated from the digital electronics used to communicate with the computer This isolation is achieved with state of the art high speed digital isolator s This isolation is provided to eliminate computer electrical noise from the ana log measurements Computers contain digital electronics that switch at high speed producing large electrical transients The computer ground functions as the return path for these transients resulting in substantial high frequency ground noise Each analog input and output channel of the LIH 8 8 has it s own separate ground path that is isolated from the computer ground Therefore computer ground noise is not coupled into the measurement Isolation is almost essential if low noise analog outputs are provided as with the LIH 8 8 If the analog outputs are not elec trically isolated from the computer ground the analog output ground will contain computer generated noise This is one of the major ways by which computer generated noise is introduced into a measurement system The isolation of the LIH 8 8 brings with it another benefit that simplifies complex measurement systems Since the analog inputs and outputs of the LIH 8 8 float with respect to ground the LIH 8 8 and the host computer are not a source of ground loop problems 1 5 4 Analog Circuitry The LIH 8 8 provides eight a
24. e HEKA software outlined above is supported on both Mac OS and Windows platforms For the scope of this discussion the only difference between the two is the mouse click For Window systems that have a multi button mouse then a left click is used For Mac OS systems that have a single button mouse a single click is used The dialog controls used in the 1 0 Control Window are as follows D ex Drag box A number within a box with a drop shadow can be changed by either a mouse drag or keyboard entry The mouse drag is accomplished by first positioning the mouse pointer over the value While holding down the left mouse button drag the mouse up or down to increase decrease the value Important note When the drag mode is active the mouse pointer will change to an up down arrow combi nation TEN When the mouse button is released the value will be set and the mouse pointer will return to it s original state Alterna tively the value can be changed via a keyboard entry by either double clicking on the value or Shift click or Right click Windows and then typing in the new value Keyboard input will terminate once the Return or Enter key are pressed Using Tab will cycle through all Drag items in the active win dow D Clear Rounded corner rectangle A text label within a rounded corner rectangle will behave like a momentary switch To activate the switch position the mouse pointer on any part of the rect
25. error windows are displayed testing should be terminated These errors are considered fatal errors Initializing Sound LIH 8 8 error LIHBBDriver dl not found v0 0 The driver is not properly installed Select Exit to terminate the program Selecting continue will force the software into Demo mode resulting in false test results Important note Please refer to trouble shooting item Driver Errors above for further assistance To open the 1 0 Control Window go to the menu bar Select the menu Window and then 1 0 Control Notebook Macros ERC Help Osciloscope Amplifier Notebook FS Macros Editor Close Front Window Ce Save Front Dialog Enable Icon Configuration The 1 0 Control window will appear The controls within this window will be used to verify the basic input and output oper ation of the LIH 8 8 http www heka com 30 Troubleshooting EE 1 0 Control Dig n OQOBOO000s8o0000000 0123456789012345 Dig Out oogooo00g0000000 Corea 282 Single Bit DA Channels 10000v 5000V 2500 5000v AD Channels 000v oov oov oov 0 000 v 0 000 Y 0 000 Y 0 000 Y g Checking the D A Outputs The most accurate way to ver ify the operation of the D A channels is with either a Digital Multimeter DMM or DVM or an oscilloscope One of the LIH 8 8 A D inputs can also be used if it is known to be working and properly calibrated i Connect the D A channel to be tested dire
26. g rate Input range Differential non linearity Drift Input impedance Signal to noise ratio No missing codes Crosstalk between ADC Crosstalk between banks Maximum over voltage 8 2 ADC multiplexed into 4 inputs Bank 0 channels 0 2 4 and 6 Bank 1 channels 1 3 5 and 7 differential isolated BNC on front panel 16 bit 1 in 65536 400 kHz aggregate 200 kHz per ADC 10 24 to 10 239 Volts 0 002 96 of FSR 2 ppm C 1 MQ 86 dB at DC to 160 kHz lt 1 mV PP 16 bit not measurable 110 dB 20 Volt 1 kHz sine wave input 35 Volts 2 2 Digital Inputs Number of channels Input type Minimum pulse width Input connectors Maximum over voltage 16 isolated 3 3 and 5 Volt logic compatible 150 ns 3 on front panel BNC all on rear panel DB 25 connector 30 Volts D C 12 Technical Specifications 2 3 Trigger Input Number of channels Input type Minimum pulse width Operational mode Input connector Maximum over voltage 1 isolated 3 3 and 5 Volt logic compatible 30 ns software selectable positive or negative edge transition BNC on front panel 30 Volts D C 2 4 Analog Outputs Number of channels Output type Output connector Resolution Settling time Output range Gain error Gain linearity Drift Output impedance Signal to noise ratio Short circuit to ground Current output 4 individual DAC pseudo differential isolated
27. he four individual 16 bit Digital to Analog converter outputs The acquisi tion software controls the D A channels output voltage and timing The D A channels have an output range of 410 24 Volts and can be simultaneously used for waveform generation The D A channels are isolated from the computer For optimal results use only high quality 15 Q BNC cables Important note Do not under any circumstances con nect an output to any of the D A channels Otherwise the D A circuitry can be damaged 2 ADC Inputs Eight BNC connectors provide access to the 16 bit Analog to Digital converter input channels The eight analog input 18 Controls and Functions channels are separated into two banks of four Each bank is mul tiplexed into one 16 bit 200 kHz A D converter The acquisition software controls the A D channel selection and sampling interval timing The A D channels have an input range of 10 24 Volts These channels are isolated from the computer For optimal results use only high quality 75 Q BNC cables Also since the A D channels are differential the BNC cables must have a very good continuous shield to both ends of the cable The shield of the BNC connector must also have a reference connected to it If the BNC connector shield is left unconnected unexpected results can occur Important note Do not under any circumstances exceed the maximum input voltage allowed of 15 Volts peak Status Indicator LED The Status L
28. http www heka com
29. iles are required This provides ease of installation and flexibility for moving the LIH 8 8 from one computer system to another 16 Installation 5 If multiple LIH 8 8 interfaces are to be connected repeat the steps as outlined above for each unit In addition for the acquisition clocks to be properly synchronized a connection between the Master clock output of one unit to the Slave clock input of the other connectors located on the rear panel must be made using standard CATS patch cables Important note Please note that the sync clock is a high frequency signal The shortest possible length patch cable should be used 6 Before powering up please recheck all connections If all connections are proper then the power LED will illuminate once the LIH 8 8 is powered ON Important note Please note that the Status LED will not be illuminated until the acquisition software has initialized the interface The LIH 8 8 is now connected and ready to go http www heka com 4 Controls and Functions 4 1 Front panel Controls o Q ZA OUTPUTS A D INPUTS TRIGGER INPUT UUTPUT B H E El D i E 3 D 5 7 N do A Au Mi Wm mi CHAS 4 O agquisitiom interfage LIH 8 o Ap STATUS o Ls o o o o o eo Figure 4 1 LIH 84 8 Front Panel Controls 1 DAC Outputs Four BNC connectors provide access to t
30. inuity tester d Disconnect any external connections to the LIH 8 8 and verify that the noise is not shown on the computer display If the noise is eliminated then start restoring the external connections one at a time while observing the noise until the culprit is found Depending on the source of the noise it may require any number of procedures to eliminate or reduce the noise These procedures may include rearranging ground connections re routing signal cables and or the removal of the noisy device Devices that tend to be noisy are monitors computer power supplies refrigerators and unshielded or poorly shielded equipment http www heka com 6 Warranty and Support 6 1 Warranty HEKA warrants to the first purchaser for a period of one year from the date of purchase invoice date that this HEKA Instrument hereafter referred to as the Product will be free from defects in workmanship and materials and agrees that it will at its option either repair the defect or replace the defective Product or part thereof at no charge to the purchaser for parts and labor The Product must be returned to HEKA properly packaged with a written explanation of the problem with transportation charges prepaid and the shipment fully insured The Customer is responsible for all duties taxes and freight charges if applicable HEKA will return the Product postpaid and insured in the most appropriate method as deter mined by HEKA If a f
31. ions including virus checkers and screen savers that are not required Hardware Verification The operation of the LIH 8 8 inter face can be verified by using the 1 0 Control Window in either the PATCHMASTER CHARTMASTER POTMASTER or EPC MASTER software The EPCMASTER software is designed specifically for testing the various HEKA amplifiers and interfaces The EPCMASTER software is available for free download from www heka com A software security key dongle is not required Basic functionality testing of the LIH 8 8 can be performed with any of the above mentioned software applications The instructions and screen images used in the following procedures are based on the EPCMASTER software The same basic instructions would apply to all of the other software applications as well First the following is a brief description of the basic input conventions used in all of the HEKA software products This introduction only covers the input types that are needed to properly execute the test procedures outlined below For a complete description please refer to the user s manual included with the software package http www heka com 26 Troubleshooting As with all GUI based software application the HEKA software prod ucts rely on the mouse or other pointing device for most of the user interaction An assumption is made that the user has a working knowledge of the operating systems various input devices and con ventions Th
32. is the capability to play a sound that is proportional to R membrane This is typically used for monitoring seal resistance This feature was originally supported via the sound output hardware of the host computer Over the years many sound board and chipset manufacturers dropped support of various commands that were used by PATCHMASTER to generate this sound This made it very difficult to support this feature other than on a few computer systems By adding the tone generator directly to the data acquisition interface this feature can now be easily supported in all computer systems without any additional hardware or software 1 5 10 Software Support The LIH 8 8 is fully supported by the following HEKA software packages e PATCHMASTER e CHARTMASTER e POTMASTER e TIDA A Windows DLL Dynamic Link Library is available for software develop ers that are interested in writing their own Windows applications Please contact HEKA for further details IgorPro XOPs external operations are also available for controlling the LIH 8 8 directly from WaveMetrics IgorPro 5 0 or later software The LIH XOP s are free of charge and can be downloaded directly from the HEKA website Please note that currently only a Windows version is available http www heka com 10 Introduction http www heka com 2 Technical Specifications 2 1 Analog Inputs Number of channels Input type Input connector Resolution Samplin
33. liability shall terminate upon the expiration of the warranty period one year from the shipping date of invoice except as to title Not withstanding any other provisions in no event whether as a result of http www heka com 6 3 Obtaining Warranty Service 37 breach of contract warranty or otherwise shall HEKA or its subcontrac tors or suppliers be liable for any special consequential incidental indirect or exemplary damages including but not limited to loss of profit or rev enues loss of use of the equipment or any associated equipment cost of capital cost of substitute goods facilities services or replacement power downtime costs or claims of Customers clients for such damages 6 3 Obtaining Warranty Service Warranty service of this Product can be obtained by returning the Product postpaid and insured to HEKA Prior authorization before shipping the product is advised for the most expedient service 6 4 Obtaining Out of Warranty Service HEKA takes pride on its superb long standing repair and maintenance service of its products In fact support is still available for the HEKA produced EPC 7 also known as List amplifier which was introduced in 1982 If a unit requires service after the warranty period has expired please contact HEKA s service department for consultation If return of the unit is necessary then shipping instructions will be provided Once the unit has been received and diagnosed
34. multaneous sampling on two channels one from each bank at 200 kHz per channel 400 kHz aggregate rate Analog input output range 10 24 Volts Four 16 bit pseudo differential analog output channels Sixteen synchronous digital input channels Sixteen synchronous digital output channels Master Slave clock circuitry for multi unit synchronization USB 2 0 host interface offers true plug and play installation Can be used with Laptop and small form factor computer systems iMac Mac mini etc Compatible with Windows 2000 XP and Vista Mac OS X 10 39 and above PPC or Intel processor 1 5 2 Hardware Configuration The LIH 8 8 is a rack mountable data acquisition unit It has BNC connectors on the front panel for the eight analog inputs the four analog outputs and four each of the digital inputs and outputs All of the digi tal input and output lines are available on ribbon cable connectors located on the rear panel The LIH 8 8 is connected to a host computer s USB 2 0 Hi Speed 480 Mbits second port over a shielded USB 2 0 cable 3 meters The LIH 8 8 is compatible with Mac OS X 10 39 and above http www heka com 1 5 Hardware Description 5 and Windows 2000 XP or Vista The host operating system treats the LIH 8 8 as it would any Flash memory device Therefore only standard operating system files are required Moving the LIH 848 from one com puter platform to another is a simple matter of connecting a USB cabl
35. n the Control section of this manual Important note Always test the condition of the fuse with either an ohm meter Digital Multimeter or a continuity tester Visual inspection may not be valid 24 Troubleshooting 2 Status LED not Illuminated The Status LED is illuminated once the application program has successfully initialized the LIH 8 8 hardware The LIH 8 8 cannot be properly initialized if the device driver or the application software is not properly installed a Check that the USB 2 0 cable is firmly attached to both the back of the interface and the host computer Make sure that the cable is not damaged b If the USB 2 0 cable is attached to a HUB then disconnect the cable and connect directly to a Hi Speed USB 2 0 port c Check that a shielded USB 2 0 cable is used Non shielded cables can lead to USB communication problems that manifest in a variety of odd behaviors d To verify that the LIH 8 8 is connected please do the following i Windows A Click on My Computer B In the section Devices with Removable Storage the device HEKA LIH88 should be visible li Mac OS X A The device HEKA LIHSS will be displayed on the desk top e Hardware Reset To properly reset the LIH 8 8 two steps are required First the power must be cycled OFF and then ON Next the USB interface must be reset by disconnecting and reconnecting the USB cable This will also cause the USB driver to unload and reload
36. nalog differential inputs four analog pseudo differential outputs sixteen digital inputs and sixteen digital outputs All channels are sampled synchronously The eight analog input channels are separated into two banks of four One bank is comprised of A D input channels 0 2 4 and 6 and the other of 1 3 5 and 7 Each bank is multiplexed into one 16 bit 200 kHz A D converter http www heka com 6 Introduction Both A D converters sample simultaneously at the maximum conversion rate resulting in a total throughput of 400 kHz If the bandwidth of the experiment calls for lower sampling rates then the hardware will adjust the rate accordingly The simultaneous sampling arrangement allows pairs of channels for exam ple the Current and Voltage signals from a Patch Clamp Amplifier to be digitized without phase shift The analysis of high frequency correlation between these cophasic signals is simplified since there is no time offset between sample points on these input channels Also during this copha sic acquisition there will be no time delay between the firing of two D A channels and the sampled inputs If more than one pair of input channels are acquired then a 5 us sampling delay will occur See illustration below I io 100 us 200 us i Kaz i AD 0 AD D Ao bo hoj LR 1 hos LR Aos tos D 3 I L ne 5 us K Ki 20 24 aah Figure 1 1 Schematic illustrating 100 us data acquisition of two pairs of input channels
37. oltage lil The measured A D input will be displayed automati cally on the rectangle boxes located under the text label AD Channels The readings for A D channels 0 to 3 are displayed from left to right on the upper row while channels 4 to 7 are on the lower AD Channels 0000Y ooov oov oov oov os00y oov oov iv If the measured value is similar to the D A output the A D channel is working properly For proper testing output voltages of 10 5 0 5 and 10 Volts should be used for each input channel Important note A minor difference between the output and input value is normal If the difference is more than a few millivolts please contact HEKA technical support for assistance Repeat the steps outlined above for the remaining A D channels Checking the Digital Outputs The most accurate way to verify the operation of the Digital Outputs is with either a DMM or an oscilloscope The LIH 8 8 Digital Inputs or A D channels can also be used if they are known to be functional i Connect the Digital Output to be tested directly to the measuring device Important note Digital Outputs 0 to 3 are avail able on BNC connectors on the front panel and can be easily accessed All of the Digital Outputs are available on a DB 25 connector on the rear panel labeled DIGITAL OUT To access these channels http www heka com 32 Troubleshooting a breakout cable will have to be f
38. puter For optimal results use only high quality 75 Q BNC cables 7 Chassis Ground A 4 mm banana receptacle provides connection to the chassis ground of the LIH 84 8 interface 8 Power Indicator LED The power LED will be illuminated when the LIH 8 8 is connected to the power line and switched ON http www heka com 20 Controls and Functions 4 2 Rear panel controls o POWER FAR FUSE DIGITAL OUT DIGITAL VO DIGITAL IN USB wer S ol aca Ti T 1 Eis UN TT o o 0 o o o O Figure 4 2 LIH 8 8 Rear Panel Controls 1 Power Switch A rocker style On Off switch is used to control the LIH 8 8 When the switch is toggled to the ON position then the green Power LED indicator on the front panel will be illuminated 2 Line Power Input A three wire male TEC connecter accepts stan dard three wire IEC female type power cords The appropriate power cord has been provided with the LIH 8 8 The internal power sup ply used in the LIH 84 8 is an auto switching multi voltage supply that will operate from 90 Volts to 250 Volts It is advisable to plug all equipment into a common outlet strip This will minimize power line induced noise sixty fifty hertz signals in the acquired data Important note To avoid a shock hazard the LIH 8 8 must be plugged into a properly grounded AC power outlet 3 Fuse A F1 0A 20 mm fuse is used to protect the internal power s
39. r use 3M electronics 8225 7003 series or equivalent Pin out assignments can be found in Appendix I of this manual Important note The absolute maximum voltage range for these inputs is 0 5 to 5 5 Volts Do not exceed this range or damage can result to the input circuitry 7 Slave Sync A RJ 45 connector provides the clock input for synchro nizing acquisition clocks between LIH 8 8 interfaces A standard CAT5 patch cable is used to connect the Master output of one unit to the Slave input of the other Important note This is a high frequency clock signal To minimize jitter and noise use the shortest length patch cable possible 8 USB 2 0 A USB 2 0 type B female connector for attaching a USB cable which enables connection to the host computer s Hi Speed USB 2 0 port Important note Only a shielded USB 2 0 cable should be used Non shielded cables can lead to USB communi cation errors Also the LIH 8 8 should only be directly connected to the computers Hi Speed USB 2 0 port http www heka com 22 Controls and Functions 9 Master Sync A RJ 45 connector provides a 33 MHz clock output for synchronizing the acquisition clocks between LIH 84 8 interfaces A standard CAT5 patch cable is used to connect the Master output of one unit to the Slave input of the other Important note This is a high frequency clock signal To minimize jitter and noise use the shortest length patch cable possible 10 Sound Output A st
40. ts The combination of these FIFOs and the high speed data transfer of the USB Bus allows high speed continuous data acquisition to be performed independent of the activity of the host com puter 1 5 7 USB 2 0 Benefits 1 5 7 1 True Plug and Play Simply connect the LIH 8 8 to an available Hi Speed USB 2 0 port on the host computer system using the supplied USB cable The computer will automatically identify the LIH 8 8 and install the appropriate system drivers if needed This connection scheme greatly reduces start up time It is no longer necessary to open the computer to add a board Simply make all of the connections to the recording setup and execute the data acquisition software Within minutes data can be acquired http www heka com 8 Introduction 1 5 7 2 Greater Computer Support In the past many data acquisition devices could only be interfaced to high end desktop computers with available bus slots i e ISA PCI etc This typically meant that only larger foot print and more expensive computer systems could be used Today virtually all desktop and laptop computers on the market provide USB ports It is now possible to use low cost small form factor systems i e iMac Mac mini etc with the LIH 8 8 for data acquisition applications 1 5 7 3 Hot Swappable Another advantage of the USB based LIH 8 8 data acquisition interface is that it can be installed or removed while the computer is running Just plug the devi
41. upply of the LIH 8 8 If the fuse needs to be replaced only replace with a 20 mm type F1 0A fuse 4 Digital Outputs A DB 25 female connector provides access to the sixteen isolated digital output lines Four of the sixteen output lines are also brought out on the front panel OUT 0 to OUT 3 All digital outputs are fully synchronous with the analog I O and are controlled by the acquisition software To mate to this connector use 3M electronics 8225 7003 series or equivalent Pin out assignments can be found in Appendix I of this manual http www heka com 4 2 Rear panel controls 21 5 Digital I O A 40 pin male connector combines the Digital Output and Input lines of the LIH 8 8 This specialized connector is re quired for interfacing with other HEKA devices such as the EPC 8 Patch Clamp Amplifier or the TIB 145 Trigger Interface To mate to this connector use 3M electronics 3417 series or equivalent Pin out assignments can be found in Appendix I of this manual Important note The Digital Output and Input lines on this connector are parallel to the main Digital OUT and Digital IN lines and not in addition to 6 Digital Inputs A DB 25 male connector provides access to the sixteen isolated Digital Input lines Three of the sixteen input lines are also brought out on the front panel IN 0 to IN 2 All Digital Inputs are fully synchronous with the analog I O and can be read by the acquisition software To mate to this connecto
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