Libra S21 and S22 User Manual
Contents
1. Press Standards F3 to see the standard curve press OK F3 to return If in linear regression mode the values for the slope intercept and coefficient of determination are printed out u Insert reference and press green run key This reference value is used for subsequent samples until changed Insert samples as required and press repeat as necessary To go back and change the parameters press Method F1 Standard Curve EHE Concentration 41 91 Reference Standards Issue 02 04 2004 Libra S21 S22 English 11 Multiwave and Equation Entry 2 5 The measurement of Absorbance Transmission values at specific wavelengths and combining these with appropriate factors is a means of overcoming interference effects in several applications By using the equation entry facility post measurement calculations can be done automatically and the end result displayed for the operator This is a very powerful facility indeed for the busy industrial QC or environmental testing laboratory Up to 5 absorbances transmittances at different wavelengths can be measured and factors applied to them an overall dilution factor can be applied to the completed equation The procedure is as follows and is best described using an example e Write the equation out in front of you ensuring there are no syntax errors Select Absorbance 1 or Transmission 2 mode Enter the title this will be shown with the result on the displa2. Libra S21 and S22 User Manual biochrom OI English Deutsch Fran ais Espa ol Italiano LED iochrom Declaration of Conformity This is to certify that the Libra S21 Visible and Libra 22 UV Visible Spectrophotometers Part number 80 2115 25 and 80 2115 20 Serial number 81000 onwards manufactured by Biochrom Ltd conforms to the requirements of the following Directives 73 23 EEC amp 89 336 EEC Standards to which conformity is declared EN 61 010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use EN 61326 1998 Electrical equipment for measurement control and laboratory use EMC requirements Signed Dated 23 October 2002 David Parr Managing Director Biochrom Ltd Postal address Telephone Telefax Biochrom Ltd 44 1223 423723 44 1223 420164 22 Cambridge Science Park Milton Road e mail enquiries biochrom co uk website http Awww biochrom co uk Cambridge CB4 OFJ England Registered in England No 3526954 Registered Office 22 Cambridge Science Park Milton Road Cambridge CB4 0FJ England CONTENTS UNPACKING POSITIONING AND INSTALLATION Essential Safety Notes OPERATION Introduction Keypad and display Basic Modes 1 Absorbance 1 1 Transmission 1 2 Factor Concentration 1 3 Ratio 1 4 Applications 2 Wavescan 2 1 Simple Kinetics 2 2 Reaction Rate 2 3 Standard Curve 2 4 Multiwave and Equation Entry 2 5
3. Methods A 4 B 5 and C 6 System Utilities Output to Printer Seiko DPU 414 1 Epson FX 80 Epson 9 pin 2 Text printer no graphics 3 HP PCL 3 4 Epson 24 pin ESC P 5 Download to Spreadsheet Messages ACCESSORIES Multiple Cell Holder Accessories Single Cell Holder Accessories Other Accessories consumables etc Acquire Applications Software MAINTENANCE After Sales Support Fuse Replacement Cleaning and General Care APPENDIX Text entry Good Laboratory Practice Least squares regression analysis and linearity SPECIFICATION AND WARRANTY Issue 02 04 2004 Libra S21 S22 English Ll SW Oo OO gt A A A ON QA Hh WN S S S SO SO S O S L NNR Fe FOO co NNN WN S LS LS NX NNN k2 AnA R 27 Unpacking Positioning and Installation e Inspect the instrument for any signs of damage caused in transit If any damage is discovered inform your supplier immediately e Ensure your proposed installation site conforms to the environmental conditions for safe operation Indoor use only Temperature 10 C to 40 C Maximum relative humidity of 80 up to 31 C decreasing linearly to 50 at 40 C e The instrument must be placed on a hard flat surface for example a laboratory bench or table which can take its weight 13 kg such that air is allowed to circulate freely around the instrument e Ensure that the cooling fan inlets and outlets are not obstru
4. e Calibration certification using filters traceable to International standards e Certificated engineers and calibrated test equipment e Approved to ISO 9001 standard Choice of agreement apart from break down coverage can Include Preventative maintenance Certification When using calibration standard filters Insert such that the flat surface is facine away from the spring end of the cell holder User maintenance is restricted to changing the mains fuse For any other maintenance operation including fitting a replacement xenon lamp contact your local supplier Fuse Replacement 1 Switch off the instrument and disconnect the power supply cord The fuse holder can only be opened if the power supply plug has been removed and is located in the power input socket on the back panel of the instrument 2 Slide the fuse holder open by pulling at the notch 3 Place fuses 1 0A 5mm x 20mm FST into the fuse holder and slide back into position 4 Reconnect the power supply cord and switch on the instrument Fuses are not normally consumed in an instrument s lifetime If they blow repeatedly contact your supplier 22 Libra S21 S22 English Issue 02 04 2004 Cleaning and General Care External cleaning Switch off the instrument and disconnect the power cord Use a soft damp cloth Clean all external surfaces A mild liquid detergent may be used to remove stubborn marks Sample compartment spillages Switch off the inst
5. absorbance value to 3 000A at 546 nm Stability 0 001A per hour at 340nm at OA Stray light lt 0 05 T at 220nm using Nal and lt 0 05 T at 340nm using NaNO2 Digital output 9 pin serial and Centronics parallel Sample compartment size 210 x 140 x 80mm Dimensions 510 x 350 x 160mm Weight 13kg Power requirements 100 240V AC 10 50 60Hz 80VA Safety Standard EN61010 1 EMC emissions EN 61326 2 3 Generic emissions EMC immunity EN 61000 4 6 Generic immunity part 1 Mains harmonics EN 61000 3 2 Quality System Designed and manufactured in accordance with an ISO9001 approved quality system Specifications are measured at a constant ambient temperature and are typical of a production unit As part of our policy of continuous development we reserve the right to alter specifications without notice Warranty Your supplier guarantees that the product supplied has been thoroughly tested to ensure that it meets its published specification The warranty included in the conditions of supply is valid for 12 months only if the product has been used according to the instructions supplied They can accept no liability for loss or damage however caused arising from the faulty or incorrect use of this product This product has been designed and manufactured by Biochrom Ltd 22 Cambridge Science Park Milton Road Cambridge CB4 OFJ UK Issue 02 04 2004 Libra S21 S22 English 27
6. output is always in black and white even on colour printers Seiko DPU 414 1 If obtained in your country it should already be configured properly If not set software DIP SW2 to American character set Epson FX 80 Epson 9 pin 2 Includes Epson FX 850 and similar Text printer no graphics 3 Use for any class of parallel printer no graphics or accents on text are printed HP PCL 3 4 Intended for printers such as HP LaserJet II HI 4 HP DeskJet 500 HP DeskJet 690C The printer must be HP PCL level 3 or greater HP DeskJet 700 820 and 1000 series printers do not fulfil this requirement and cannot be used Use for letter or A4 sized paper European Epson 24 pin ESC P 5 For use with Epson 24 pin dot matrix printers and older inkjet printers such as the Stylus 400 Output is automatic when the key is pressed and auto print in Preferences is on If auto print is off results can be printed on demand using the key 16 Libra S21 S22 English Issue 02 04 2004 Download to Spreadsheet Results can be downloaded directly to Excel when the PC has the Spreadsheet Interface Software installed 80 2112 23 and the two are linked with the serial cable 80 2105 97 detailed instructions are supplied with the software Thus absorbance wavelength data comprising a scan for example can be picked up as columns of numbers and converted to a more conventional graph using the spreadsheet results can then be formatt
7. 2 Instrument description for example asset number operator name and replacement group names for Methods A B and C for example application types or operator name if a multi user environment can be entered here To enter a name press appropriate key on keypad to cycle through options of lower case letter numbers and upper case letters for example pressing key cycles through abc2ABC Preferences 3 Set your preferences as follows Sample number prompt no yes enables entry of sample number between 1 999 prior to running an experiment rather than starting from Sample 1 again e Autoprint on off if off results can be printed manually using key Printer Default graph scale 0 3 0 2 0 1 0 0 5 and Autoscale e Confirm exit from application no yes Key click on off 14 Libra S21 S22 English Issue 02 04 2004 GLP 4 Refer to Appendix for more information This option determines whether GLP is on or off in terms of printing and reporting the results the calibration interval for GLP however is always on and can be done automatically at pre defined time intervals always on daily weekly monthly quarterly If GLP is on the results are printed automatically after calibration they can also be printed on demand using Print F2 on the System page Note that the GLP print out will show the date for when the full calibration was done Calibrated and that this can be different
8. ance difference per unit time as opposed to the absorbance difference per se The correlation quality of line fit is calculated from 10 equally spaced absorbance time points during the course of the experiment The procedure is as follows Enter appropriate wavelength and press OK F3 Select time units seconds 1 or minutes 2 Enter delay time or lag time if applicable and press OK F3 Enter the duration of the assay and press OK F3 Enter factor required to convert slope to meaningful units and press OK F3 Insert reference and press green run key x This reference value is used for subsequent samples until changed Insert samples as required and press repeat as necessary The assay is shown graphically as it proceeds and reverts to show The result total change in absorbance over the reaction time as defined by the intercepts multiplied by the factor slope and the line quality a coefficient of determination of gt 95 is expected if the assay was carried out over a linear section The slope is always presented as Abs min even in seconds mode Start and final absorbances as well as absorbance difference To see the assay on the whole display press Graph F3 to return press OK F3 Data points can be viewed by pressing Data F1 moving the cursor F2 and F1 To go back and change the parameters press Method F1 Reaction Rate Reaction Rate CHE 0 000 0 004 0 206 De
9. cks its integrity for GLP purposes The GLP test of this instrument is essentially a confidence test that it is performing as it was when manufactured and tested For absolute measurements an annual certification service agreement with your supplier is recommended The integrity of the instrument for GLP purposes is quantified from the calibration status of the instrument the bandwidth this is assessed during calibration by measuring the zero order beam width the wavelength accuracy by comparing to the 881 9 nm xenon emission line the values of built in absorbance filters compared to when the instrument was manufactured or last serviced by an accredited engineer the instrumental stray light The expected values are given in parentheses on the GLP print out after calibration the range of acceptance is defined by the technical specification of the instrument In the unlikely event that the instrument fails calibration or goes out of specification a message will appear on the display In this event the following should be checked is the cell compartment lid closed properly is asample in the light beam if so remove it is the baseplate plug in place single cell accessory is the in fill panel at the front of the cell compartment in place Pressing OK after the message GLP Calibration Fail appears confirms that you have accepted the instrument status If you are working in a regulated environment s
10. cted position at least 2 inches from the wall e This equipment must be connected to the power supply with the power cord supplied and must be earthed grounded It can be used on 90 240V supplies e Switch on the instrument and check that the display works see Operation e To enter laboratory name operator name instrument asset number details and current date time refer to System Utilities If this equipment is used in a manner not specified or in environmental conditions not appropriate for safe operation the protection provided by the equipment may be impaired and instrument warranty withdrawn 2 Libra S21 S22 English Issue 02 04 2004 Essential Safety Notes There are a number of warning labels and symbols on your instrument These are there to inform you where potential danger exists or particular caution is required Before commencing Installation please take time to familiarise yourself with these symbols and their meaning Caution refer to accompanying documents Background colour is yellow symbol and outline are black AN WARNING AN WARNING UV RADIATION UV RADIATION IS HARMFUL TO YOUR EYES HOT If power is restored with this cover removed eye protection must be worn Accessories e Care should be taken when handling all heated accessories e Ensure that the cell compartment lid is closed when operating cell changers and the sipper e It is essential that the baseplate plug supplied with single cell accessori
11. ders have the option of being used as a single cell holder This means that there will be no rotation after pressing run Description 4 position cell changer 8 position water heated cell changer 6 position Peltier heated cell changer 8 position cell changer Part number 80 2106 01 80 2 109 70 80 2 106 04 80 2108 01 Comments Accommodates cells 10 50mmm in pathlength Requires a water circulating bath Locate round extension of tube restrainer into top of cell changer thumb screw Thread tubes through the tube guide and attach this to the instrument base using the screws provided Replace the front blanking plug on the cell compartment lid with the new one that is provided Requires Temperature Control Unit 80 2112 49 Insert into socket 1 Spare if required Libra 21 S22 English Issue 02 04 2004 Single Cell Holder Accessories e Install by removing accessory in place replacing if necessary the baseplate plug supplied and positioning the single cell holder so that the arrow is on the front face and it locates in place Then push the finger locks backwards so that they lock into position Press the accessory button on the home page Description Comments Cell holder 10mm pathlength 80 2106 05 Cell holder for sample stirring Requires magnetic flea and controller Cell holder 50mm pathlength 80 2106 07 Cell holder 100 mm pathlength Cylindrical cell holder Up to 100 mm pathlength cylin
12. drical cells Water heated cell holder 80 2 106 08 10 40 mm pathlength Requires a water circulating bath Replace the front blanking plug on the cell compartment lid with the new one that is provided HPLC cell holder 80 2106 11 Flowcell volume is 8 ul pathlength is 2 5mm Thread wires through one hole of the tube guide and attach this to the instrument base using the screws provided Replace the front blanking plug on the cell compartment lid with the new one that is provided Peltier cell holder 80 2106 13 Set required temp in range 20 49 C Insert into socket 2 Electrical cell holder 80 2106 12 Set required temperature off 25 30 37 C Insert into socket 2 Issue 02 04 2004 Libra 21 S22 English 19 Other Accessories consumables etc Description Part number Comments Sipper 80 2112 25 Use if a large number of samples for single readings is required Requires single cell holder 80 2106 05 or 80 2106 13 10mm flowcell and tubing supplied together with separate user instructions Temperature Control 80 2112 49 Required to supply the extra power Unit required by the 6 position Peltier heated cell changer 80 2106 04 Printer stand 80 2112 18 For thermal printer Dust cover 80 2106 19 Spare Consumables and other items Pump head tubes 6 for Sipper 80 2080 74 PTFE flowcell tubing with connectors 80 2055 13 Replacement flowcell including tubing 80 2080 60 Autosampler Interface ki
13. ed or manipulated as appropriate prior to inclusion in reports or archiving saving to hard disk Results from all modes of use on the instrument can be output in this way Output is automatic when the 07 key is pressed Messages Most messages are self explanatory and relate to use of the instrument Others relate to the calibration of the instrument on switch on This instrument has One or more of the parameters tested for during GLP calibration failed 1 or more GLP is out of specification see Appendix You can accept this tests status and continue to use the instrument as normal but you may to contact your local service engineer Failed to calibrate properly contact local service engineer Failed to calibrate properly contact local service engineer Failed to align filters Failed to calibrate properly contact local service engineer Failed to calibrate properly contact local service engineer Issue 02 04 2004 Libra 21 S22 English 17 ACCESSORIES If an accessory is changed press the accessory button on the home page F2 to initialise the instrument in order that the appropriate accessory can be identified Depending on the accessory type a list of options is presented Multiple Cell Holder Accessories e Install by removing accessory in place replacing with the new one turning the central mounting screw until it is finger tight and pressing the accessory button on the home page e All multiple cell hol
14. es is fitted to optimise air flow and to prevent light ingress Issue 02 04 2004 Libra S21 S22 English 3 OPERATION Introduction Your spectrophotometer is a stand alone simple to use instrument with a high resolution liquid crystal display LCD and a comprehensive range of spectrophotometry measurements can be undertaken It works on the basis of light from the xenon lamp being directed by a fixed mirror through the monochromator inlet slit This passes through one of several dependent on wavelength selected filters mounted on filter quadrant the filtered light is then directed onto the holographic grating which produces light of the selected wavelength The light then leaves the monochromator via the exit slit and mirrors focus and direct the light into the sample compartment This passes through your cell containing the sample of interest and then a defocusing lens to a solid state detector unit The resulting signal is then filtered and displayed Your spectrophotometer has the following capabilities e Basic Modes for Absorbance Transmission Factor Concentration Absorbance Ratio e Application Modes for Wavescan Wavelength Scanning Simple Kinetics Reaction Rate Standard Curve Multiple Wavelength Multi Wavelength Equation Entry e 18 user defined methods in 3 groups of 6 Methods A B C e Print results from the instrument display in graphical format e Download of results directly to Excel fo
15. llows e Enter appropriate wavelength and press OK F3 Select time units seconds 1 or minutes 2 e Enter the duration of the assay and press OK F3 Enter the time interval minimum 2 maximum 60 seconds Select if the actual absorbance time data should be printed with the results e Insertreference and press green run key e This reference value is used for subsequent samples until changed Insert samples as required and press repeat as necessary To see the assay on the whole display press Data F3 to return press OK F3 e Data points can be viewed by moving the cursor F2 and F1 this enables the identification of slope start and end times for example e To go back and change the parameters press Method F1 NOTE This mode should be used to check sample stabilisation prior to kinetics studies for example since the xenon lamp is not a continuous output source unlike deuterium and tungsten lamps Issue 02 04 2004 Libra S21 S22 English 9 Reaction Rate 2 3 Reagent test kits are routinely used for the enzymatic determination of compounds in food beverage and clinical laboratories by measuring NAD NADH conversion at 340 nm The change in absorbance over a specified time period can be used to provide useful information when an appropriate factor defined in the reagent kit protocol is applied Note that reaction rate and enzyme activity can be calculated if the factor used takes account of the absorb
16. lta A Slope Quality 0 206 0 004 oF ao Method Reference 10 Libra S21 S22 English Issue 02 04 2004 Standard Curve 2 4 The construction of a multi point calibration curve from standards of known concentration in order to quantify unknown samples is a fundamental use of a spectrophotometer a common example is the Bradford determination for proteins This instrument has the advantage of being able to store this curve as a method The procedure to construct the standard curve is as follows Press Standards F3 followed by New F1 and confirm F3 this step is not necessary if this mode is being used for the first time Enter appropriate wavelength and press OK F3 Select Curve Fit method Single Point 1 Linear Regression 2 or Linear Interpolation 3 Enter number of standards 2 12 and press OK F3 Enter number of replicates 1 3 and press OK F3 Enter concentration of first standard and press F3 To include a zero concentration standard include this in the number of standards to be entered and enter 0 00 for concentration use a blank when required to enter standard 1 Enter concentrations of other standards as prompted Insert reference and press green run key This reference value is used for subsequent samples until changed Insert standards as required and press Q followed by OK F3 repeating as necessary to construct the standard curve Values can be written down if required
17. nd press OK F3 Enter end wavelength range 200 or 335 900nm and press OK F3 Select scan speed as appropriate slow 1 medium 2 fast 3 or survey 4 The scan speed depends on the wavelength range due to the wide range in baseline energy and this in turn affects data interval so the figures are nominal Select if the peak check table is required if selected a table of wavelengths and absorbance maxima for up to 20 peaks can be printed out _ Nominal scan speed nm min Slow 250 Medium 750 1800 3000 e Insert reference and press green run key to obtain reference spectrum e This reference spectrum is used for subsequent samples until changed Insert samples as required and press repeat as necessary e Press Data F3 to access data points these can be viewed by moving the cursor F2 and F1 a peak is indicated by a flag symbol e For rapid movement press 4 6 to go to left right side of the graph or 5 to go the centre Press 2 to zoom in 8 to zoom out e To go back and change the parameters press Method F1 Wayvescan 8 Libra S21 S22 English Issue 02 04 2004 Simple Kinetics 2 2 Simple kinetics studies to investigate the shape of an assay curve can be readily performed The wavelength of interest is entered together with the time interval at which absorbances are to be read the results are displayed graphically simulating a chart recorder output The procedure is as fo
18. nter the space Press 1 repeatedly until 1 appears Press 0 to enter 0 Press OK F3 to confirm the name entry To enter the equation Abs511 12 5 Abs 720 0 3 100 Use lt to remove any entries still there Press F2 twice to enter Press F1 1 to enter the first absorbance Al wavelength value is defined later Press F1 3 to enter the sign Enter numerical factor 12 5 using the keypad press F3 Press F2 to close the first bracket Press F1 2 to enter the minus sign Press F2 to enter Press F1 2 to enter the second absorbance A2 wavelength value is defined later Press F1 3 to enter the sign Enter numerical factor 0 3 using the keypad press F3 Press F2 twice to close the brackets Press F3 to confirm the equation is correct The two wavelengths for Al and A2 now have to be defined enter 511 and 720 when prompted The dilution factor 100 now has to be entered enter 100 Libra 21 S22 English Issue 02 04 2004 Good Laboratory Practice Good laboratory Practice GLP concerns being able to trace experimental results to an instrument an operator and the time the result was obtained so that a laboratory can prove that the Instrument was functioning correctly or not Laboratory operator and internal instrument reference names can be entered on the spectrophotometer If the GLP option is on during calibration or re calibration the instrument self che
19. o a blank this can be air The procedure is as follows e Enter appropriate wavelength and press OK F3 e Insert reference and press green run key The cell changer if fitted automatically moves to position 2 and displays the result for the reference measurement 0 000 Xenon lamp based instruments are press to read whereas deuterium tungsten lamp instruments measure continuously Thus to monitor sample stabilisation the simple kinetics mode must be used e This reference value is used for subsequent samples until changed Insert samples as required and press repeat as necessary e To go back and change the wavelength press Method F1 Transmission 1 2 Transmission mode measures the amount of light that has passed through a sample relative to a blank this can be air but displays the result as a percentage The procedure is as follows e Enter appropriate wavelength and press OK F3 Insert reference and press green run key e This reference value is used for subsequent samples until changed Insert samples as required and press repeat as necessary e To go back and change the wavelength press Method F1 Factor Concentration 1 3 Concentration mode is used when a conversion factor is known and converts the absorbance measurement for a sample at a specific wavelength into a concentration by a simple multiplication of absorbance x factor The procedure is as follows e Enter appropriate
20. r manipulation and archiving via a serial interface lead to a PC e Selftest diagnostics for GLP purposes A range of accessories further enhances the capability of the instrument The home page provides access to user modes system utilities and accessory identification and set up 4 Libra S21 S22 English Issue 02 04 2004 Keypad and display Press the soft key on the keypad directly below the corresponding option on the display F1 F2 and F3 to select that option For example on the home page above e press F1 to take you to System Utilities e press F2 to identify the type of cell changer holder that has been fitted e press F3 to toggle the display back light on off display contrast can be changed within System F1 Press to print result if auto print is off or to re print result if auto print is on to back space in order to correct text and characters in appropriate boxes to start making measurements and print results green run key ot to stop making measurements or entering parameters and return to the home page use as an escape mechanism red stop key Press the corresponding number on the keypad to enter the user mode choices for example 1 followed by 1 is Absorbance mode whereas 2 followed by 4 is Standard Curve Mode Issue 02 04 2004 Libra S21 S22 English 5 Basic Modes 1 Absorbance 1 1 Absorbance mode measures the amount of light that has passed through a sample relative t
21. rument and disconnect the power cord The cell holders baseplate and sample compartment are all coated in a chemical resistant finish Strong concentration of sample however may affect the surface and spillages should be dealt with immediately Observe all necessary precautions if dealing with samples or solvents that are hazardous There is a small drain hole in the sample compartment to allow excess liquid to drain away Liquids will drain onto the bench or table under the spectrophotometer or if preferred this drain hole can be connected to waste using suitable tubing Remove the cell holder and clean separately Use a soft dry cloth to mop out the sample compartment Replace the cell holder Reconnect the power cord and switch on the instrument Issue 02 04 2004 Libra 21 S22 English 23 APPENDIX Text entry The following example shows how to enter a title and equation in Multiwave The principles are identical however for other text entry options such as Method Names e To enter the title Copper 10 Use lt to remove any text still there Press 2 repeatedly until C appears 66 99 Press 6 repeatedly until o appears Press 7 repeatedly until p appears Press F2 to move to next place Press 7 to enter a second p 6699 Press 3 repeatedly until e appears Press 7 repeatedly until r appears Press to initiate entry of a space Press F2 to move to next place then F2 again to e
22. t 80 2104 96 Serial interface cable for connection to PC 80 2105 97 D9 male instrument to D9 PC Spreadsheet Interface Software 80 2112 23 Centronics parallel printer interface cable 80 2071 87 Separate information giving details on serial and parallel interface connections if required is available from a Service Engineer with your local supplier whom you should contact for further details 20 Libra S21 S22 English Issue 02 04 2004 Acquire Applications Software Acquire comprises application modules for wavelength scanning reaction kinetics quantification multi wavelength time drive and can be used to enhance the software already included on the spectrophotometer 80 2115 31 Acquire Applications Software Wavelength Scanning Reaction Kinetics Quantification Time Drive Multi Wavelength Recommended PC for proper operation For optimum performance an IBM compatible 486 or greater personal computer running Microsoft Windows 95 98 or NT is required The PC should have a minimum of 8MB RAM 200Mb hard disk a 1 44 MB 3 5 inch floppy disk drive a serial mouse installed and free COMMS serial port and VGA graphics Any printer supported by Microsoft Windows 95 can be used Contact your supplier for further information Issue 02 04 2004 Libra 21 S22 English 21 MAINTENANCE After Sales Support We supply support agreements that help you to fulfil the demands of regulatory guidelines concerning GLP GMP
23. to the date of instrument operation Date this is shown on the example below If the date is the same Calibrated shows the time that it was done instead Press More F3 on the system page to view the GLP results on the instrument display Libra S22 GLP Report Instrument Libra 22 Operator A T Dadd Date 22 March 2002 Time 10 00 17 Serial No 79500 Version 6090 V1 0 Calibrated 22 March 2002 Instrument Life 25 6 Hours Service 22 March 2002 Bandwidth 2 0 3 0nm 2 9 PASS Wavelength Accuracy 881 9nm 1 nm 881 9 PASS Absorbance Accuracy 2200m 11603 ed ole 1 772 PASS 340nm 1 633 1 665A 1 649 PASS 500nm 1 477 1 491A 1 484 PASS Stray Light 220 nm lt 0 05 0 021 PASS Language 5 Select language for the display and print out Service 6 This is for accredited service engineers only and requires the entry of a pass code Issue 02 04 2004 Libra S21 S22 English 15 Output to Printer The graphics capability of the instrument means that the following requirements for printer compatibility should be fulfilled e The printer must not be USB only style parallel Centronics is required e The printer must not be designed to work with MS Windows only GDI type these are less expensive printers and can only function when connected to a PC with the appropriate driver installed If in doubt check with the printer manufacturer Note that printer
24. uch as a drug discovery laboratory that generates data for GLP GMP activities or reports you should not use the instrument and contact your local service engineer Issue 02 04 2004 Libra 21 S22 English 25 Least squares regression analysis and linearity The slope or best straight line and intercept in a kinetics assay or standard curve determination is calculated from a least squares linear regression of the data The following equations are used where n is the number of data points 2 12 yn 2 gt ne Intercept gt y gt as slope n Slope Linearity is an estimate of the goodness of fit of the least squares linear regression analysis a perfect fit being 100 It is used in both the Reaction Rate and Standard Curve modes and is expressed by a coefficient of determination r calculated using the following equation Quality 100 _ a 26 Libra S21 S22 English Issue 02 04 2004 SPECIFICATION AND WARRANTY Wavelength range 190 1100nm for Libra S22 325 1100nm for Libra S21 Maximum scanning speed 3000 nm minute Spectral bandwidth lt 3nm Wavelength accuracy Inm Wavelength reproducibility 0 5nm Light source xenon lamp Detectors two silicon photodiodes Photometric range 3 000 to 3 000A 9999 to 9999 concentration units 0 1 to 200 T Photometric accuracy 0 5 or 0 003A to 3 000A at 546 nm whichever is the larger Photometric reproducibility within 0 5 of
25. ugh abc2ABC Note that a space is entered using key 1 which cycles between 1_1_ Press another key to move to next letter To enter a doubled letter eg AA or number eg 00 press gt F2 and then the appropriate key again e Delete incorrect characters using Complete entry by pressing OK F3 e An example of name entry is given in the Appendix Issue 02 04 2004 Libra S21 S22 English 13 System Utilities Date 11 November 2003 GLP Calibrated 7 November 2003 Time 12 41 Instrument Life 164 5 Hours Operator Service 27 February 2002 Serial Number 987654 Software Version 6090 V1 4 Copyright 1999 2001 Biachrom Ltd Software developed for Biechrom Ltd bu 4narksoft Ltd and P M Dickerson M4 Calibration Normal After selecting the system option F1 on the home page there is initial information including the calibration status of the instrument and the date of the last full GLP calibration see above The GLP calibration details can be printed out for record purposes by pressing F2 if required note they are printed automatically depending on the specified GLP calibration interval see below Set up To adjust the contrast of the display to suit lighting conditions press Contrast Y or Contrast to decrease or increase F1 or F2 respectively Clock 1 Press OK F3 to cycle through year month day hour minute and use F1 or F2 to adjust the parameter down or up as appropriate Customise
26. wavelength and press OK F3 Enter known factor range 0 01 9999 and press OK F3 e To enter a negative factor press F1 the reference should have a higher absorbance than the samples Insert reference and press green run key e This reference value is used for subsequent samples until changed Insert samples as required and press repeat as necessary e To go back and change the wavelength or factor press Method F1 6 Libra S21 S22 English Issue 02 04 2004 Ratio 1 4 This facility enables the determination of Abs 1 Abs 2 and Abs 1 factor e Enter the first wavelength e Enter the second wavelength e Select if background correction for both wavelengths is required Ifyes enter the wavelength e Enter the factor to be applied to the first wavelength e Enter dilution factor Insert reference and press green run key e This reference value is used for subsequent samples until changed Insert samples as required and press Q repeat as necessary e To go back and change the wavelength or factor press Method F1 Issue 02 04 2004 Libra S21 S22 English 7 Applications 2 Wavescan 2 1 An absorption spectrum can be obtained from your instrument this enables simple identification of peak height and position A reference scan has to be obtained first The procedure is as follows Select Absorbance 1 or Transmission 2 mode Enter start waveleneth range 190 or 325 890nm a
27. y and print out so should be descriptive see Appendix e Enter the equation see Appendix Insert reference and press green run key This reference value is used for subsequent samples until changed Insert samples as required and press repeat as necessary e To go back and change the parameters press Method F1 Multiple Wavelength Copper 10 11 27 Method Reference 12 Libra S21 S22 English Issue 02 04 2004 Methods A 4 B 5 and C 6 After defining parameters in any of the applications and prior to measuring a sample a method can be saved To save a method e press stop to return to the home page select one of the three method banks 4 5 or 6 press save F1 and choose an unfilled method by pressing the appropriate number enter the method name see below and press OK F3 A stored method is available as an option directly on the instrument menu To change parameters the method must be deleted first To delete a method e press stop to return to the home page select one of the three method banks press delete F2 and select the required method by pressing the appropriate number you are asked to confirm this Entry of alphanumeric characters for print outs and method names e Remove default characters if necessary using lt e Press appropriate key on keypad to cycle through options of lower case letter numbers and upper case letters for example pressing key 2 cycles thro
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