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AlgaeWatch operation manual

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1. SAMPLE OUT FEMALE PLUG LUER LOCK o 9 TURNER DESIGNS 5 algaewatel WARNING o Tror e E WAY VALVE SAMPLE INLET 1 4 MALE NPT Fa AX 20 5 83 REAR MOUNTING PATTERN ON BOX CONDUIT PLUGS Figure 2 AlgaeWatch Front View External Step 1 Step 2 CALIBRATION Calibration Procedure The Calibration Procedure will take approximately 5 minutes The cleaning procedure will take approximately 10 minutes Refer to Figure 2 on previous page for locations of items referred to in this procedure For further reference a diagram of the screens and a brief summary of the System Values can be found on page 27 Bubbles trapped in the syringe during injection of the blank or calibration standard are a possible cause of instrument error With the syringe ina vertical position tap the syringe against a solid object to move the bubbles to the needle end of the syringe Then force the bubbles out by pushing a small amount of solution through the needle end of the syringe Close the two way valve flowcell shut off valve The valve is closed when the handle is horizon
2. Nanay JIdWYS OL sw 009250 or 8 84 6651 10 OFF OD3 834 353199 SNOISIAJA aep 51 88 SANIT 0006 VS ValL dO gt 234024 SNOLLDJNNOO 1VOINYHO3W INIOd JIdWYS 0006 AVSVal JO JILL SNOILO3S 33 357314 5323 383338 LINN 5 13110 3 8 X 1 414 OAd 7 1 35012 1 NOINN DAd 06 1 1 1920 250 6850 0 0011 2650 DAd REV 1 2 4560 866 ON OMA XE AlgaeWatch ONY Jdid HIOS OL 35 TIM ONIN3IHOLYIAO AYYSSIODINNN S Q3Q V33lHL 215 ANNOS DIYW 8 SI NY SLIHOIL 8 SNANL 012 14 4 ONIGWN1d 11 318W3SSV OI ONISWfITd 433 NO 30119 ONIGWN1d ONILNNOW TIVM Q3Q33N SV HLONAT OL 1 448 1 333 3lddIN OL Z daa 1 4 035012 WOX ONISWIld 55 2 Sd YXM
3. 1 2 ce OUT CAP Figure 6 AlgaeWatch Front View External 40 AlgaeWatch REAR MOUNTING PATTERN ON BOX Sj 5 U SAMPLE OUT gt TURNER DESIGNS FEMALE 7 PLUG 3 LUER LOCK S po i WAY VALVE algaewatel Turner Designs Algae Monitor 1 4 WARNING 1 14 82 I 4X 20 5 83 CONDUIT PLUGS REV 1 2 Definitions 1 LCD Digital Display This liquid crystal display LCD shows the screens and continuously displays the HOME screen when values are not being entered or viewed D Except during calibration the contrast of the LCD can be adjusted any screen by 2 pressing the UP DOWN ARROW Keypad The keypad is used to enter new unit values and to move between screens Once the User or Master Identification has been entered if the keypad is not used for 15 minutes the unit will automatically return to the HOME screen Main Power Switch This is the main power switch for the entire unit When ON the LCD will illuminate AC Power Circuit Breakers There are two 3 15 amp circuit fuses located inside the lower enclosure
4. settings the greater the resolution During an alarm condition the 4 20 mA output will still send out the fluorescence signal During calibration the 4 20 mA output will send out a 4 mA signal From the HOME screen press lt 5 gt to enter change the sixth System Value User ID lt 5 gt User ID Change For security a USER ID is required to change the System Values or calibrate the unit The original or default value is 8520 To change the USER ID Press lt 5 gt Key in Master ID 1962 Press lt ENT gt Key in new User ID Press lt ENT gt Press lt HOME gt From the HOME screen press lt 6 gt to view the seventh System Value AC Relay Status lt 6 gt AC Relay Status Displays the current activation status of AC Relays A and B This screen is for visual status only The AC Relays are used to control an external device activation state cannot be altered from the screen Press lt 6 gt Status of AC Relays A 5 Press lt HOME gt Setting the Real Time Clock O For future reference record all set up values in Appendix A Before changing the date or time be sure to download any data from the data logger 7 algaewatch From the HOME screen press lt gt to access the date time and AC relay alarm values 0 the datalogger to reference the correct time the hour of day must be entered Only 1 12 numerical values will be accepted Press C from HOME
5. 22 Pre Installation Installation 3 Service Assistance Power amp Utilities Required 3 Returned Goods 24 Required Tools amp Accessories 3 Specifications Accessories 25 Location amp Sampling Point 3 WARRANTY soot erepti 26 Mechanical Connections 4 Electrical Connections 4 APPENDICIES START UP otras 5 A CONFIGURATION SHEET 28 Start Up Procedure 5 B INSTALLATION CHECKLIST 29 Monitoring 6 C FIRMWARE FUNCTIONS 30 CALIBRATION 10 D SCREENS FLOW CHARTS 32 Calibration Procedure 10 System Value Screens Notes on Calibrating 11 User ID Screens 32 Calibration Data Screen 12 Diagnostic Screens 32 Table 1 Calibration Data 12 Time Date Alarm Screens 33 INTERNAL DATA LOGGER 13 Data Logger Screens 33 Parameters 13 Calibration Screens 34 Software Installation 13 E TROUBLESHOOTING WORKSHEET 35 Running IDL Software 14 E FIGURES 37 Examining Downloaded Data 15 In vivo chlorophyll concepts 42 PREVENTATIVE MAINTENANCE 16 Cleaning Basket
6. OLS ONISN SONILLIJ CI3QV33IH1 333 NOWI 456 0009 N d YSNANL JOY AT8W3SS Y 38 Figure 5 AlgaeWatch Front View 14 82 9 AC OUTPUT 2 G 8 AC OUTPUT 2 N 1 7 OUTPUT 2 lt gt 4 5 OUTPUT 1 6 OUTPUT 1 7 51 1 AC POWER IN lt gt 7 2 AC POWER IN lt gt AC POWER IN G 1 14 C DC OUTPUT D DC OUTPUT L EFLOWSWIN F FLOW SW GND AC CONNECTIONS CONNECTIONS 1 AC POWER IN lt H gt Reserved G Reserved 2 AC POWER IN lt N gt B Reserved H Reserved 3 AC POWER IN lt gt C DC Output Reserved 4 AC OUTPUT lt H gt D DC Ouput J Reserved 5 AC OUTPUT lt N gt E Flow Sw In K Reserved 6 AC OUTPUT 1 lt G gt F Flow Sw GND L Reserved 7 AC OUTPUT 2 lt H gt 8 AC OUTPUT 2 lt N gt 9 AC OUTPUT 2 Dimensions are in inches 39 AlgaeWatch REV 1 2 Figure 6 AlgaeWatch Front View External SERIAL DATA PORT LCD UPPER ENCLOSURE LOWER ENCLOSURE TERMINAL STRIP POWER SWITCH
7. BLANK SOLUTION Using the blank solution syringe flush the flowcell with 60 ml of blank solution via the Luer lock Injection Port Then inject another 60 ml blank solution and allow it to remain in the flowcell by leaving the syringe attached Step7 Press ENT BLANK XXXX REV 1 2 Step 9 Step 10 Any value less than 25 is acceptable If the blank is over 25 and lt 0 gt is pressed you will receive an error message Press lt ESC gt to abort the calibration and check the blank solution When reading is stable press lt O gt The unit will display a flashing WAIT wait message in the lower right hand corner of the screen while the unit registers the blank Step 12 Then the screen will display To continue Press lt ENT gt Press lt ENT gt CALIBRATION SOLUTION Using a clean syringe flush the flowcell with 60 ml of Turner Designs calibration solution see Replacement Parts pg 17 via the Luer lock Injection Port Then inject another 60 ml calibration solution and allow it to remain in the flowcell by leaving the syringe attached Press ENT CAL SOLUTION XX Wait for the reading to stabilize The CAL SOLUTION should be 1 10 When the CAL SOLUTION is stable and between 1 and 10 press lt gt The unit will display a WAIT wait message in the lower right hand corner of the screen while it registers the calibration solution Then the screen will displa
8. the alarm condition is corrected 5 During an alarm condition the 4 20 mA will still send out the fluorescence signal Table 2 System Function Alarms Alarm Delay Alarm Normal min Condition Lamp ON F A PDA REV 1 2 System Function Alarm Definitions a Lamp F A Indicates the status of the excitation light source Reports whether the lamp is OFF or ON If the power is ON and the lamp is good the diagnostic screen will display Lamp ON b No Sample Flow N F If there is a problem with the sample flow lasting for the 10 minute delay period a N F alarm will be noted The flow switch used is rated at 0 5 minimum flow tolerance is 0 4 0 6 gpm If there is a N F alarm check terminal connections to the flow switch Check the sample feed lines and the unit s flowcell for any restriction Electrical connections should be performed only by trained personnel To determine which alarm is currently active press lt ESC gt from the HOME screen and the alarm status screen will appear You may also view the lamp and flow status in real time by accessing the diagnostic screens The diagnostic screens are accessed by pressing lt gt from the HOME screen and then lt ENT gt See Diagnostic Screen flowchart pg 31 If the Lamp displays OFF and there is power to the unit contact the Turner Designs Technical Support Table 3 High Signal Alarms Alarm High
9. the in vivo data is calibrated by correlation with other measurements FLUOROMETER The sample water containing algal cells is measured as a side stream of the water passes through a polished glass tube in the detection system An excitation light source shines across the glass tube the chlorophyll a in the water fluoresces and a photodiode reads the emitted light The quantity of light emitted is proportional to the amount of chlorophyll a present in the system see Figure 1 below The AlgaeWatch consists of a microprocessor based fluorometer with electrical inputs outputs for a flow switch 4 20mA signal and data logging It is engineered to ensure reliability and pre assembled to simplify installation Plumbing and calibration accessories may need to be acquired see Replacement Parts page 17 for a list of recommended 1 algaewatch parts and Figures 3 and 4 pages 36 and 37 for the plumbing diagrams UNITS Because in vivo chlorophyll detection is a relative measurement we have not included units on the AlgaeWatch screen or in the logged data However if correlation between the AlgaeWatch signal and extracted chlorophyll analysis is conducted the AlgaeWatch can be calibrated to estimate actual chlorophyll concentration in micrograms liter ug L please see Appendix G for greater detail THEORY OF OPERATION Excitation PE Emission By ALight Source ii Photodiode Water Sample Flow Figure
10. 8 30 a m 5 00 p m Pacific Time 24 AlgaeWatch REV 1 2 SPECIFICATIONS AND ACCESSORIES Specifications Power Relays Signal output Dimensions Weight Enclosure Sample Flow Maximum Water Pressure Plumbing Inlet Pipe Size Outlet Pipe Size Environment Ambient Temperature 90 250 VAC 200 250 VAC 50 60 Hz 5 amps 90 250 VAC 200 250 VAC 50 60 Hz 5 amps fused at 3 15 amps ea Type F One 4 20 mA isolated 8 W x4 D x 15 20cm x 10cm x 38cm 5 105 2 3 kg Approximates NEMA 4X 100 psi 14 NPT Male 14 NPT Female 40 F to 120 F 4 C to 49 Maximum sample temperature 140 F 60 C Relative Humidity Table 5 System Default Values and Ranges SYSTEM VALUE Cal Soln Value Background Value High Signal Alarm Level 4 mA Output 20 mA Output Software Version Master ID User ID AC Cycle Time AC On Time 0 100 ACCESS KEY DEFAULT la MN 0 0 000 to 998 000 lt 2 gt 0 004 10 999 000 lt 3 gt 0 000 10 998 000 999 000 lt clock gt lt 7 gt 1 to 1440 min 25 AlgaeWatch REV 1 2 Accessories Included with Unit Flowcell brush Terminal strip tool Operating manual 2 spare clean out port caps Datalogger cable Datalogger disk Spare desiccant plugs Suggested Optional Accessories Calibration Kit P N 6500 900 Kit includes Calibration Standard 1L syringes flowcell brushes Plumbing Kit 6500
11. Press 0 Key in the hour of day 1 12 Press ENT Press lt 5 gt to return to clock menu 1 AM PM For the datalogger to reference the correct time morning or evening AM PM must be entered Use lt ENT gt to toggle between AM PM Press from HOME Press lt 1 gt Press lt ENT gt to toggle between AM PM Press lt ESC gt to return to clock menu lt 2 gt Minutes For the datalogger to reference the correct time the number of minutes after the hour must be entered Press from HOME Press 2 Key in the minutes after the hour 0 59 Press ENT Press lt ESC gt to return to clock menu Month For the datalogger to reference the correct date the month of the year must be entered Press from HOME Press lt 3 gt Key in the month 1 12 Press lt ENT gt Press lt ESC gt to return to clock menu lt 4 gt Date For the datalogger to reference the correct date the day of the month must be entered Press lt gt from HOME Press lt 4 gt Key in the day 1 31 Press lt ENT gt Press lt ESC gt to return to clock menu REV 1 2 lt 5 gt Year For the datalogger to reference the correct date the year must be entered Press from HOME Press lt 5 gt Key in the year 00 99 Press ENT Press lt 5 gt to return to clock menu 6 AC Cycle Time Controls the frequency in which AC Relay Outputs are activated Range from 10 minutes to 96
12. The AlgaeWatch is rated for light industrial environments Do not install within 10 feet 3 meters of devices such as large generators that generate a strong electromagnetic field It is extremely important to eliminate air entrapment in the sample line The best way to accomplish this is to sample from the center of the pipe or from the side of the pipe The unit should not be installed in direct sunlight this could cause the internal temperature of the unit to be significantly higher than ambient and produce errors or damage the components Note The maximum environment temperature is specified at 120 F 49 C D Do not mount this instrument on vibrating walls or surfaces Damage can occur to critical components REV 1 2 Mechanical Connections Refer to Figure 6 Appendix F for the location of the required mechanical connections Two inch NPT pipe connections provided for inch PVC pipe hook up The sample outlet line is 1 4 inch female the sample inlet line is 4 inch male shut off valve If the Plumbing Accessory Kit is purchased the outlet line is 1 4 inch female to connect to user supplied 1 4 inch male piping the inlet line is gt inch NPT female to connect to user supplied 1e inch male piping see Figures and 4 on pages 36 37 IMPORTANT Sample discharge should flow to an unrestricted drain Pipe rises greater than 10 feet 3 m should be avoided Mounting eyelets
13. 1 Fluorescent Detection Theory of Operation Fluorescence data can be used to supply an indication of algal growth in the sample water Although the fluorescence data is not quantitative in nature see Appendix G a robust relationship between the fluorescence signal and actual chlorophyll concentration can be easily determined through a comparison with extracted chlorophyll samples However in many instances it may be more useful to develop a relationship between the fluorescence signal and another measure of algal growth such as cell counts or filter run times In other cases the relative fluorescence signal may be all that is needed to make informed decisions The power of the AlgaeWatch System is in the sensitivity of the fluorescence measurement and it s ability to continuously monitor the algal community which is constantly changing sometimes very rapidly REV 1 2 AlgaeWatch is designed to be easily interfaced into external logging or data collection software packages It has an analog 4 20mA signal that can deliver real time fluorescence data to an external logging system The system also has a 20 000 point internal data logger with user selectable data logging intervals Logged data can be downloaded to a PC using the RS 232 data output port see Internal Data Logger section pg 13 2 algaewatch Data Interpretation Upon installation of the AlgaeWatch the fluorescence data should be compared to data fr
14. 955 includes inlet and outlet plumbing kits Warranty Terms Turner Designs warrants the AlgaeWatch and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions e Turner Designs is not responsible for replacing parts damaged by accident or neglect Your instrument must be installed according to instructions in the User s Manual Damage from corrosion is not covered Damage caused by customer modification of the instrument is not covered e This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for incidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state Damage incurred in shipping is not covered Warranty Service To obtain service during the warranty period the owner shall take the following steps 1 Write email or call the Turner Designs Technical Support department and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com 2 Carry out any adjustments or tests as suggested by the Technical Support Department 3 If proper performance is not obtained you will be issued a Return Materials Authorization numb
15. Background Value To determine the appropriate background value filter a sample of water through a GF F or membrane filter to remove all algal cells Next inject the filtrate into the AlgaeWatch using the syringe provided Assuming that the default calibration is active secondary standard set to 500 take the fluorescence value of the filtrate and plug into the following formulae Filtrate fluorescence X 5 Background Fluorescence Press lt 1 gt Press lt ENT gt Key in the Background Value Press lt ENT gt Press lt HOME gt From the HOME screen press lt 2 gt to enter change the third System Value High Signal Alarm Level lt 2 gt High Signal Alarm Level If the fluorescence level rises above this level and remains there for a 5 minute delay period a high signal alarm will be triggered The alarm triggers the 5V DC signal output which can be used to control external devices such as a light siren or external control device Please also see the section on Alarms page 18 Press lt 2 gt Press lt ENT gt Key in the High Signal Alarm Level Press lt ENT gt Press lt HOME gt Initially you will most likely want to leave the High Signal Alarm disabled until you become familiar with the typical fluctuations in chlorophyll levels Once this is established you will know recognize abnormally high signal levels and then be able to set the alarm appropriately 6 algaewatch From the HOME sc
16. Signal S H Range Default 0 2 999 999 19 algaewatch High Signal S H If the fluorescence signal rises above the user set level see Table 5 page 24 and remains there for the 2 minute delay period a S H alarm will be noted If there is a S H alarm check if the High Signal Alarm Level is set too low Verify that calibration has been performed properly REV 1 2 TROUBLESHOOTING Introduction Because the AlgaeWatch system includes hardware software and chemistry it is important to collect all the diagnostic data first To facilitate data collection each AlgaeWatch unit is shipped with the Troubleshooting Worksheet E After collecting the data requested by the worksheet most problems can be solved over the phone with the assistance of Turner Designs Technical Services see Service Assistance Returned Goods page 24 for contact information When using this guide it is assumed that all problems associated with an alarm have been resolved first Something as simple as a clogged basket strainer can lead to other alarm messages which could all be solved at one time simply by cleaning basket strainer Generally speaking if there is no System Function Alarm F A alarm this is persuasive evidence that the electronics of the instrument are functioning properly In that case it is likely that any problem is either mechanical or has resulted from another system problem or from the operator s unfam
17. Strainer 16 FIGURES amp 5 Calibrating a 16 Figure 1 Fluorescence Detection 1 Cleaning Flowcell 17 Figure 2 Front View External 9 Biofouling 17 Figure 3 Inlet Plumbing 37 Replacement Parts 17 Figure 4 Outlet Plumbing 38 ALARMS 18 Figure 5 Front View Internal 39 Alarm Delay a 18 Figure 6 Front View External 40 Alarm Activation 18 as 41 Multiple 18 0 algaewatch REV 1 2 INTRODUCTION CHLOROPHYLL FLUORESCENCE AlgaeWatch is a fluorescence sensor designed to monitor the level of chlorophyll containing cells in fresh water The algaewatcti AlgaeWatch 86665 continually senses gt the concentration of chlorophyll a an indication of algal biomass in the sample water Chlorophyll a the primary photosynthetic pigment in all plants is a fluorescent molecule Where chlorophyll containing organisms are small enough as with phytoplankton or microalgae fluorescence may be measured directly in vivo chlorophyll detection without extraction or chemical treatment For many types of qualitative work in vivo measurement alone may answer the experimenter s questions For quantitative determinations
18. as blank solution If you are not using commercial distilled water check your source of blank solution against distilled water for 11 algaewatch REV 1 2 background fluorescence DO NOT USE SAMPLE WATER AS A SOURCE OF BLANK SOLUTION Use of the wrong blanking solution can result in inaccurate fluorescence values 8 Use only Turner Designs fluorescence calibration solution see Calibration Solutions in the Replacement Parts page 17 These calibration solutions meet strict specifications that are difficult to achieve when the solutions are made at the customer s site Not using the Turner Designs solutions could result in inaccurate fluorescence values 9 All solutions are injected with a syringe into the stainless steel Luer lock Injection Port Depress plunger at a steady slow rate 10 Use a separate syringe for the blank and calibration solutions 11 Avoid injecting bubbles into the unit Bubbles trapped when injecting the blank and calibration solution are a possible cause of instrument error With the syringe in a vertical position tap the syringe against a solid object to move the bubbles to the needle end of the syringe Then force the bubbles out by pushing a small amount of solution through the needle end of the syringe 12 The LEFT ARROW may be used to return to previous calibration screens 13 During the calibration sequence MAKE SURE to wait for BLANK and CAL SOLUTION readings to stabilize be
19. calibration sequence by pressing the lt ESC gt key While viewing a System Value press lt ENT gt to access the screen to change that value After entering a new System Value press lt ENT gt to accept the new value 3 User Identification To change System Values or to calibrate the instrument a four digit USER ID is required For security a MASTER ID different from the USER ID is required to view or change the USER ID 30 AlgaeWatch REV 1 2 31 Once an ID has been entered if the keypad is not used for 15 minutes the unit will automatically return to the HOME screen The ID will have to be entered again before the unit values can be changed or calibration can be performed Fluorescence Display After the unit is powered up or after calibration the fluorescence displayed will not react immediately but will respond after a delay of about 10 seconds LCD Contrast The contrast of the Liquid Crystal Display can be adjusted on any screen except during calibration by pressing the UP and DOWN ARROWS AlgaeWatch REV 1 2 APPENDIX 0 Screens Flow Chart Systems Values From the HOME screen press key to view value To change value press lt ENT gt while viewing input ID enter new value and press lt ENT gt again Press HOME screen Home Screen shows view or change User ID AlgaeWatch fluorescence signal Master ID is required and whether data logger and alarms are active Cal Soln value Relates th
20. device using the AC Relays Make sure the device itself is powered on and operational Replace fuse with spare fuse located in the spare fuse holder on the PCB 23 AlgaeWatch REV 1 2 POSSIBLE CAUSE SOLUTION Background value ineffective This is not usually cause Do not attempt to change current i e HOME screen reading for alarm as the HOME settings unless you are certain does not change when the screen reading is a result something is wrong Consult with Background value is of a combination of the Turner Designs Technical factors Services Unit does not respond to System Values Check that the correct calibration solutions incorrectly entered Background Test Value and Cal Soln Value have been entered see Appendix A SERVICE ASSISTANCE RETURNED GOODS Turner Designs experienced technical staff is available to assist you in troubleshooting the AlgaeWatch unit However should you need to return anything for the unit a Returned Materials Authorization RMA must be obtained from Turner Designs Please call prior to returning any equipment The use of an RMA minimizes the potential for administrative delays and facilitates prompt turn around Turner Designs Technical Support Telephone 877 316 8049 Outside U S 408 749 0994 FAX 408 749 0998 E mail support turnerdesigns com Hours
21. hours Press from HOME Press Press lt gt to toggle Omin 96hrs Press lt ESC gt to return to clock menu 7 AC On Time Controls the length of time the AC Relay Outputs stay on when they are activated Range from 0 100 of the AC Cycle Time Press lt gt from HOME Press 7 Press lt gt to toggle 0 100926 8 algaewatch Press ESC to return to clock menu For Example If you would like to use the AC Relay to control sample source using an automated valve you may want to sample raw water containing algal cells for 30 minutes every hour In this case you would set the AC Cycle Time to 60minutes and the AC On Time to 5096 The result would be that the valve would allow treated municipal water to run through the system for 30 minutes every hour thus limiting the effects of biofouling and raw sample water would flow through the system for 30 minutes an hour The AlgaeWatch unit start up has now been completed CAUTION After start up wait a minimum of 15 minutes before calibrating the unit to allow the unit to come to equilibrium REV 1 2 Figure 2 AlgaeWatch Front View External SERIAL DATA PORT N UPPER ENCLOSURE LOWER ENCLOSURE TERMINAL STRIP POWER SWITCH 1 2 E
22. is most likely related to the System Values entered for the site If for example a Cal Soln Value of 200 was erroneously entered the unit s numerical calculation of the sample reading might exceed 999 NOTE Examine Possible Cause Solution in the numbered order 2 Incorrect calibration 1 Check the Calibration Data screen Recalibrate the unit be sure to use the correct calibration solution and that the reading is between 196 10 Check the expiration date of the solution HOME screen displays 1 Possible optics Check the FS reading in the OVER not blinking from over problem Improper or Diagnostics sequence to OVER deteriorated filters A steady OVER is a different symptom than a blinking over OVER and indicates that the sample reading is too high for the unit s light detector This is related to the chemistry of the sample and displays that the sample readings are too high for the unit at the current sensitivity level NOTE Examine the Possible Cause Solution in the numbered order 22 POSSIBLE CAUSE 2 Incorrect calibration 3 System Values are incorrect i e Cal Soln Value etc Check the Configuration Record SOLUTION Recalibrate the unit making sure that you are using the correct Calibration Solution and that it reads between 1 and 10 Check the expiration date of t
23. shown is less than 25 wait for number to stabilize then press lt 0 gt 7 After WAIT command disappears unit will prompt you to press lt ENT gt to continue 8 Press lt ENT gt 9 Inject 120 ml of calibration solution flush with 60 ml then allow portion of second 60 ml to remain in flowcell Press lt ENT gt 10 If the number shown is between 1 and 10 wait for reading to stabilize then press lt gt WAIT command will appear 11 When finished unit will prompt you to press lt ENT gt to continue 12 Press lt ENT gt 13 Press lt 1 gt to accept the new calibration settings Unit returns to calibration menu Open valve to start sample flow by returning lever to the vertical position Calibration is finished AlgaeWatch REV 1 2 AlgaeWatch TROUBLESHOOTING WORKSHEET The Troubleshooting Worksheet is designed to facilitate diagnostic data collection After collecting all data on the worksheet most problems can be solved over the telephone Refer to the Service Assistance Returned Goods Section for the appropriate numbers Please fill out worksheet completely All entries are important GENERAL INFORMATION Unit Serial _ Tag is located beneath sample block Field Contact Name _ Field Contact Phone _ Description of Symptoms Date Time Symptoms Started Describe Any Physical Damage To Unit Date Of Last Calibration Press ENT from HOME scree
24. the screen above then lt ENT gt to toggle the chosen relay ON and OFF The device should go on when ON is selected and off when OFF is selected If it does not then be sure to check the device itself first then check the terminal strip connections trained personnel ONLY When the test is finished return to the HOME screen and device control will revert to control by current unit values This function can serve as a manual override for device control tests While on this screen you can turn a device on and off regardless of the unit values previously entered 4 From the above screen press lt ENT gt Oper XXXX Hrs Lamp ON Flow ON Oper This indicates how many hours the unit has been in operation since installation Lamp This indicates whether the LED light source is operating properly Flow This indicates whether the flow is ON OFF 5 From the above screen press lt ENT gt Blank X X Cal std XXX X 21 algaewatch REV 1 2 Troubleshooting Guide REMEMBER handle any alarms FIRST SYMPTOM POSSIBLE CAUSE SOLUTION HOME screen displays 1 System Values are 1 Check the Configuration over OVER blinking from incorrect i e Cal Soln Record for the site Access the over to OVER Value etc System Values and verify that they are entered correctly A blinking over OVER is a different symptom than a steady OVER and indicates that the sample reading exceeds 999 This
25. when the alarm is first activated the unit will return to the HOME screen display the ALM message in the upper left hand corner Pressing the lt 5 gt key will display the current alarm Take the appropriate action to clear the condition see Troubleshooting page 20 When the condition triggering the alarm is cured ALM will disappear from the HOME screen Alarms cannot be aborted without curing the problem Multiple Alarms If multiple alarms are triggered alarms will be listed on the alarm screen when lt ESC gt is pressed from the HOME screen Note alarms are not listed in the order they occur For example the alarm screen might display bili 18 algaewatch Alarm History The alarm history can be viewed by pressing LEFT ARROW from the HOME screen This shows which alarms have been activated since the alarm history screen was last cleared To clear this screen press five times while the alarm history screen is displayed No alarm since last reset will be displayed Notes About Alarms No alarms are monitored when the unit is turned OFF 2 Certain alarms are not monitored during calibration see Notes on Calibrating page 11 3 When the unit is first powered up the HIGH SIGNAL alarm will begin to be monitored 5 minutes after start up Thus a problem or spike during start up will not mistrigger the HIGH SIGNAL ALARM 4 All alarms will be reset automatically if
26. TURNER DESIGNS algaewatcn Turner Designs Algae Monitor Operation Manual September 7 2014 P N 998 6050 Revision 1 2 TURNER DESIGNS 845 W Maude Avenue Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 To ensure proper system operation Turner Designs strongly recommends reading this manual in full After reading the entire manual please review the following Prior to installation completely review the Pre installation Installation Checklist located in Appendix B CAUTION AlgaeWatch should only be used with PVC plumbing kit P N 6500 955 as supplied JND Prior to start up completely review the Start Up Section PARN located on page 5 CALIBRATION Prior to calibrating the AlgaeWatch completely review the Calibration Procedure located on page 10 Prior to calling Turner Designs for assistance completely review the Troubleshooting Guide located on page 22 TROUBLESHOOTING TABLE OF CONTENTS Alarm History 18 Notes About Alarms 18 INTRODUCTION 1 Definitions etes 19 THEORY OF OPERATION 1 Data Interpretation 2 TROUBLESHOOTING 20 SPECIFICATIONS 3 20 3 Troubleshooting Guide
27. Two spare fuses are provided within the instrument enclosure Inlet Line Shut off Valve This valve is used to direct the sample flow to the unit When the handle is vertical the valve is open and sample flows into the unit When the handle is horizontal sample flow is stopped permitting calibration solutions to be injected into the unit via the Luer lock injection port Luer lock Injection Port During instrument calibration standard and blank solutions are injected into the unit using a syringe at the Luer lock connection adjacent the valve The unit is calibrated using a secondary standard dilution and a blank solution distilled water Cap This cap permits access to the flowcell for cleaning with a brush when flushing with acid alone is not effective Sample In This is where the sample intake line is attached to allow sample to flow through the unit Sample Out The sample exhaust line attaches here There MUST be a valve at this point if there is backpressure on the line which is open during normal operation If there is back pressure on the line close the valve during calibration be aware that some solution will flush to the floor 10 Sample Block This houses the flowcell and optical filters To change the flowcell or filters the Sample Block must be replaced 11 Power Terminal Strip Located behind the bottom enclosure front panel Power AC out 41 flow switch and 4 20 mA chart recorder connectio
28. action We suggest anytime there are suspected or expected changes in the environment or water quality that you collect samples You may find you will have different in vivo chl extractive chl correlations for different field stations or for different times of the year For best results keep the collected sample cold and in the dark until you can filter them Filter the samples as soon as possible within a couple of hours of collection and freeze At the exact time of sample collection note the in vivo chlorophyll fluorescence value of the particular sample and label 4 In the lab extract the collected in vivo samples as per the EPA 445 0 protocol and analyze your extracts with calibrated fluorometer or spectrophotometer Determine the correlation between the extractive and vivo data Further information can be found on our website EPA link for 445 0 protocol http www turnerdesigns com customer care fluorometer application notes epa method 445 fluorometer application notes Effects of turbidity on in vivo chlorophyll fluorescence http www turnerdesigns com t2 doc appnotes S 0035 pdf Frequently Asked Questions http www turnerdesigns com alqaewatch faqs menu 43 AlgaeWatch REV 1 2
29. ank Cal std REV 1 2 Figure 3 AlgaeWatch Inlet Plumbing Appendix F FIGURES MALONE INON s ea rx cn oa X oo Nn lM INNI HOIVMIVOVY Tm aw wo JIVAANNT m ame 7 2 WIM gt 2812313 HOLIMSAYOT3 1N3INCPHISNI 21 3 919 OL 0960966 zwa SOTO X b L 11914 52 75 1 F ddiN 00607290 2 5 ETT OT TINY a 14 7 1 Wd 5 HOUMS 1198 NION 2 1 5020591 1 91 951851 Gi E 0090008 1181 dd d PYA TIM ONIN3IHOLH3AO AHVSSIO JNND NOILO3NNO2O O3UV 3HHT SUSY ANNOS O1 QIHINOIH SI IHU 8394 5 2 O1 2 10 ONIN NY 41 SM HOS ONEN OL 434 ONY 6 44 SHXTIOH O1 A 884155 ON BINT Md HOLY 434 NONN Ol HOnOMWHI OL 138 3A TVA ONIBIATTd 318NGSSV 2 Sd vu E OI 2 SNB 5 OIOXTVCPIBE ITV TY 81 334 34VI NOTA qvum t 9956 00592 HINAN YOY 4 1 3 1 5 4 55 REV 1 2 AlgaeWatch 37 Figure 4 AlgaeWatch Outlet Plumbing 1301 ME 40 3 1560 866 O d LINO LIA ONIgWfTId VSN 98076 V2 5 5 5 ME INIOd
30. are an integral part of the plastic housing Mounting the unit at eye level is recommended Electrical Connections Electrical connections should be made only by trained personnel Refer to Figure 5 pg 38 for the Terminal Connections showing the terminal strip location and configuration of the required electrical connections for the power pump flow switch and 4 20 mA connections To access the terminal strip TURN OFF MAIN POWER TO THE INSTRUMENT AT CIRCUIT BREAKER then remove the lower enclosure face 4 screws There are two terminal strips within the instrument 1 9 are for AC connections and A L are for input output connections 4 algaewatch To connect a wire to the terminal strip TURN OFF MAIN POWER TO THE INSTRUMENT AT CIRCUIT BREAKER Loosen the proper terminal screw screwdriver provided and insert wire from below into terminal Tighten screw firmly A termination legend is provided on the backside of the enclosure door for reference To disconnect a wire TURN OFF MAIN POWER TO THE INSTRUMENT AT CIRCUIT BREAKER Loosen the termination screw and pull the wire out of the terminal When finished carefully insert the ribbon cable onto the PCB and replace enclosure face with the 4 screws D Signal wires and power wiring should NOT be run in the same conduit Failure to separate or shield these wires will result in electrical interference REV 1 2 START UP Before start up the following ite
31. ce measurement The default value of 15 from Table 1 on page 12 is recommended unless you are establishing a correlation to a known concentration as described in Appendix G Before entering changing the first System Value the unit will prompt for the User and the screen will read Please input ID Enter a valid USER ID default is 8520 Press lt ENT gt Key in the Calibration Solution Value Press lt ENT gt Press lt HOME gt From the HOME screen press lt 1 gt to enter change the second System Value Background Value lt 1 gt Background Value This value is the background fluorescence for the sample Background fluorescence can be caused by materials in the sample water that have a similar fluorescence signature to chlorophyll a Examples of interfering compounds include dissolved organic matter accessory algal pigments and degraded chlorophyll pheophytin High concentrations of interfering compounds can result in a slight increase to the fluorescence signal A means of compensating for this background fluorescence is to estimate the contribution of the interfering compounds to the fluorescent signal and enter the level of interference as the background value The system will then automatically subtract the entered background level from the REV 1 2 fluorescent reading The degree of background fluorescence varies from site to site Unit must be calibrated prior to setting the
32. e closed the outlet valve is then open to the atmosphere see Figures 4 and 5 pages 37 38 3 CAUTION After injecting acid solution into the flowcell via the luer lock port be sure to flush it out completely BEFORE removing the clean out cap 4 Remove the clean out cap and open the clean out valve horizontal position Dip the flowcell brush into the dilute acid solution and insert it gently into the clean out opening 5 Slide it gently up and down in the opening to remove any coating on the glass cuvette REV 1 2 6 Close the clean out valve vertical position and replace the flowcell clean out cap 7 Turn on the flow Flow is ON when the valve handle is vertical 8 Turn ON the Main Power Switch 9 Calibrate the unit after allowing it to warm up for 15 minutes refer to Calibration Procedure page 10 Biofouling Sample water containing bacterial and algal cells will be in contact with the glass flowcell of the AlgaeWatch System Because of this a biofilm will gradually begin to grow on the flowcell and at some point the film will thick enough to interfere with the fluorescent readings The rate of fouling will change from site to site The AlgaeWatch has an optical compensation system that will correct for fouling to a certain point Beyond this it is the user s responsibility to clean the flowcell on a regular basis to prevent corruption of the data There are several ways in which the rat
33. e from the side of the water line to avoid air entrapment 3 Provide a free unrestricted drain for the sample stream preferably to the tower basin no back pressure max 10 ft 3 m rise CUSTOMER REQUIREMENTS ELECTRICIAN 1 Ensure that the environment will support a NEMA 4X type enclosure 2 Provide 90 250 VAC 50 60 Hz 5 amp electrical service to the AlgaeWatch 3 If the unit s 4 20 mA output signal will be used check to see if an isolator should be purchased 4 Terminate flow switch wiring on terminal strip refer to Figure 5 pg 38 FINAL REQUIREMENTS CUSTOMER 1 Obtain needed materials calibration solution distilled water dilute acid calibration kit flowcell cleaning brushes Customer s Responsibilities This checklist outlines the work that is required prior to start up Work is necessary to ensure quality and proper operation However if any of these requirements cannot be met contact Turner Designs Technical Services In some cases alternative procedures will still provide reliable results 29 AlgaeWatch REV 1 2 FIRMWARE FUNCTIONS The unit has a software interface that simplifies calibration and changes of unit values See Screens Flow Charts pages 31 33 The following descriptions of the unit s software functions will help provide a better understanding of the unit 1 Screens Built into the unit are a series of computerized screens which are cal
34. e of biofouling can be slowed 1 Flow rate As the flow rate in the flowcell increases the rate to which biofouling organisms and attach and grow on the flowcell walls will decrease The minimum flow rate required is 0 5 gpm Flow rates in the range of 2 5 gpm could significantly decrease flowcell fouling rates 2 Flowcell cleaning The AlgaeWatch has been designed to allow quick and easy cleaning of the flowcell A regular schedule should be established in order to prevent the establishment of biofouling organisms on the flowcell Replacement Parts 6500 900 6000 970 6000 350 6000 119 6000 910 120 0110 119 0103 17 algaewatch Calibration Kit Desiccant Plugs Pkg 3 Flowcell Brush Pkg 3 Data Cable Syringes Pkg 10 Flow Switch Basket Strainer REV 1 2 ALARMS Alarms have been built into the unit to warn about conditions relating to high algal levels and internal instrument functions There are two types of alarms for AlgaeWatch System function alarms Fluorometer lamp and No Flow F A and N F alarms See Table 2 High Signal alarms See Table 3 page 19 Refer to Table 5 page 25 for default values Alarm Delay To avoid unnecessary triggering of alarms the condition must be in effect for a certain delay period See Tables 2 and 3 Alarm Activation When an alarm is triggered ALM will blink in the upper left hand corner of the HOME screen From any other screen
35. e value of the tracer standard to a fluorescence measurement Background Value Background fluorescence for system 7 Set fluorescence Lk value that once exceeded will set tte Diagnostic Screens High Signal Alarm access unit s diagnostic screens from HOME press 3 output Output can be set to correspond to a certain fluorescence signal TURNER DESIGNS 6500XBL 1 0 0302 20mA output XXX XXX Raw XXX XX ENT View alarms triggered SH NF since last reset 1 Test ON 2 Test RlyB OFF If alarm is triggered N F shows which alarm s ESC Oper XXX Hrs Lamp ON Flow ON 1 4mA XXX 2 20MA XXXX Blank XX X Cal std 32 AlgaeWatch REV 1 2 Screens Flow Chart 1 From the HOME screen press lt B gt or lt gt to access the following functions For example to change the clock settings or alarm functions first press lt gt then the number of the function to be changed Key in the new value and press lt gt then lt ESC gt to return to the clock menu Time Date amp Alarm Screens Datalogger Screens 0 to 02 21 99 Datalogger lt 7 gt 07 58 30AM lt 0 gt lt 3 gt Hour 7 Status Stop New lt ENT gt to toggle 1 Interval 1min lt ENT gt to toggle toggie Download data Min 0 5x 8 to start New Erase data Month 2 5x 9 to start New Downl
36. er RMA to reference Package the unit write the RMA number on the outside of the shipping carton and ship the instrument prepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be repaired and returned free of charge for all customers in the contiguous continental United States 26 AlgaeWatch REV 1 2 For customers outside of the contiguous continental United States who purchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Designs pays for return shipment custom duties taxes and fees are the responsibility of the customer Out of Warranty Service Follow steps for Warranty Service as listed above If our Technical Support department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs Inc 845 W Maude Ave Sunnyvale CA 94085 27 AlgaeWatch REV 1 2 APPENDIX CONFIGURATION RECORD Date Configured Technician Serial Number System Value Range Configuration Background Value 0 0 to 100 0 Cal Solution Value 0 000 to 998 000 Hi
37. fore pressing the appropriate key on the keypad 14 DO NOT allow the calibration solution to sit in the flowcell for longer than necessary approximately 2 minutes 15 To abort the calibration and maintain the current calibration settings press lt ESC gt before step 9 is completed The unit will prompt 12 algaewatch lt 1 gt Abort Cal lt ESC gt Continue Press lt 1 gt to abort 16 entire calibration procedure must be completed for the new values entered during calibration to be store Calibration Data Screen This screen provides a check on proper calibration It is accessed from the HOME screen by pressing lt ENT gt then lt 2 gt Table 1 Calibration Data Blank Cal Std Access Key lt ENT gt amp lt 2 gt Default 0 0 15 0 Range 0 250 0 1 000 Blank Shows raw data output for blank solution as set during calibration It can be used to check proper calibration 0 Blanking capability of the instrument is 25 e g maximum blanking of raw data is 250 0 Cal Std Shows raw data output for the standard solution as set during calibration It can be used to check proper calibration REV 1 2 INTERNAL DATA LOGGER AlgaeWatch is equipped with an internal data logger to record the unit s output Data is saved in a compressed binary BIN format to be downloaded and converted to ASCII data with the Internal Data Logger IDL software Data Logge
38. from the data logger main menu Download data 5x lt 8 gt to start the AlgaeWatch press 8 five times to start downloading data The PC will display a bar graph and data block countdown The AlgaeWatch will display Download data Data Blks XX If there is an error in downloading data the following screen will be displayed Comm error lt ESC gt to retry a If the error screen appears press lt 5 gt and make sure the serial cable is securely connected and operational b Make sure the correct serial port has been selected IDL software main menu c Check to make sure that the AlgaeWatch date and time functions have not been changed for the current data logged d Verify that you completed steps 1 7 Correct screens must be displayed on both the computer and AlgaeWatch algaewatch 8 When downloading is finished IDL will ask you to name the file and select the path folder for the downloaded file Click on Browse to change the name or path or you can accept the default name test bin and path Then click on OK to return to the Main Menu If you would like to wait until later to convert the data to ASCII format in order to save disk space then skip to step 12 To convert the data to an ASCII file now go on to step 9 9 To convert a BIN file to a regular ASCII file PRN file from the PC click on the Convert Downloaded Data File to ASCII File IDL will then ask you
39. gh Signal Alarm Setpoint 0 004 to 999 000 4mA Output 0 000 to 998 000 20mA Output 0 002 to 999 000 AC Cycle Time 10min 96hours AC On Time 1 to 100 of AC Cycle Time User ID 4 digit 28 AlgaeWatch REV 1 2 APPENDIX B Pre Installation Installation Checklist The following checklist is provided so the appropriate preparations may be made prior to equipment start up Completion of the listed items is mandatory to assure proper installation and a properly functioning piece of equipment 1 Check sample water background fluorescence Background lab test code 2 Check that the turbidity is less than 150 NTU 3 Assure water temperatures of 32 140 F INSTALLATION REQUIREMENTS CUSTOMER 1 Locate the unit within 125 feet from the sample point 2 Locate the unit out of direct sunlight 3 Locate the unit where ambient temperatures are 40 120 F 4 49 C 4 Locate the unit at least 10 feet 3 meters from devices such as large generators which require a great deal of electrical power or generate a strong electromagnetic field CUSTOMER REQUIREMENTS PLUMBER 1 The sample stream must be plumbed to the unit to deliver at a rate gt 0 5 between 0 5 and 1 25 gpm is optimal and lt 100 psi One Ve inch and one NPT pipe connections both female are provided for PVC pipe hook up refer to Figure 3 and 4 page 36 37 Ensure that sampling point will avoid air entrapment 2 Sampl
40. he solution Access the System Values and verify they are entered correctly HOME screen displays minus sign negative readings i e sample is reading less concentrated than blank as set during last calibration 1 Fouled flowcell 2 Calibrated with contaminated blanking solution or the calibration solution was used instead of the blank solution Thoroughly clean and rinse flowcell with recommended solution using the brush if necessary See Cleaning the Flowcell page 16 Recalibrate HOME screen reads zero System Values incorrectly set Make sure valid System Values entered see Appendix A Screen blank or black LCD s screen contrast too high or too low If screen is blank adjust contrast by pressing UP ARROW screen is black use the DOWN ARROW continuously until screen is visible Use UP and DOWN arrows to fine adjust Unit does not calibrate Failure to complete entire calibration procedure You must press 1 at the end of the calibration sequence for the unit to accept the values Recalibrate AC Out is not operating properly 1 Problem with terminal strip connections or device itself 2 Fuse has blown If there is an alarm check the High Signal Alarm for 0 5V signal problems Refer to Diagnostics page 20 AC Out test function to test if the unit is properly controlling the
41. iliarity with the unit The troubleshooting procedure works best in this sequence 1 Handle any alarms see Alarms page 17 2 Determine whether or not the System Values have been entered correctly see recorded values in Appendix A and the System Default Values in Table 5 page 24 3 Perform the Diagnostics procedure as described on the following page 4 Determine whether or not the chemistry is behaving as expected Does the blank read close to zero on the HOME screen and calibration solution read between 1 5 on the calibration screen Check the Cal data screen see Calibration Data Screen page 11 to determine whether the last calibration seems correct 5 Complete the Troubleshooting Worksheet Appendix E 6 Contact the Turner Designs Technical Support see Service Assistance Returned Goods page 24 Diagnostics AlgaeWatch contains diagnostic screens and functions to aid in troubleshooting These functions are accessed from the HOME screen by pressing lt gt then ENT to page through the series of 4 screens Press the LEFT ARROW to return to a previous screen or or to return to the HOME screen 1 From the HOME screen press lt gt TURNER DESIGNS 6500XBL 1 0 0302 This screen displays the version number and release date of the firmware installed in the instrument 2 From the above screen press ENT Raw The signal output is the output f
42. led up using the keypad and shown on the digital display 1 1 Screen Once the unit has been activated the HOME screen is continuously displayed except when accessing other screens From the HOME screen access the calibration data and the calibration sequence by pressing lt ENT gt Other screens are accessed from the HOME screen by pressing various keys on the keypad Go to the HOME screen by pressing the lt HOME gt key except during the calibration procedure To return to the HOME screen from calibration first press lt ESC gt to abort the calibration sequence 1 2 Warning Screens There are warning screens throughout the software that inform of invalid entries for values or ID 1 3 Alarms When an alarm occurs ALM blinks in the upper left hand corner of the HOME screen The nature of the alarm can be discovered by pressing lt ESC gt from the HOME screen see Alarms page 17 and Troubleshooting page 19 2 Keypad Functions 2 1 Left Arrow The LEFT ARROW can be used to correct typing errors when data is being entered or changed It acts as a backspace or delete key During calibration it can be used to return to previous screens in the sequence if you wish to re run the calibration It is also used from the HOME screen to view the alarm history 2 2Up and Down Arrows From HOME screen can be used to change screen contrast 2 3 Escape and Enter You can escape to the previous screen or abort the
43. ms should be procured O Calibration Solution P N 6500 900 ordered shipped separately from unit O Distilled Water O Dilute Acid not included O Plumbing Kit P N 6500 955 ordered shipped separately from unit Items listed above as ordered sent separately do not automatically ship with an order Start Up Procedure Bring the sample flow to the unit Check for leaks in the plumbing Switch the Main Power Switch under the enclosure face see Figure 2 page 9 to the ON position The LCD will illuminate When the power is first turned on an ID screen will appear for a few seconds showing the firmware version and date After 10 seconds or press lt ENT gt or lt gt for immediate access the HOME screen will appear The HOME screen will display the uncalibrated chlorophyll fluorescence value 3 digits If the screen is blank try adjusting the screen contrast using the UP and DOWN ARROWS SAMPLE HOME SCREEN XXX AlgaeWatch During start up all system values should be recorded in the configuration record located in Appendix A Entering System Values amp Definitions From the HOME screen press lt 0 gt 10 enter change the first System Value Calibration Solution Value 5 algaewatch For future reference a diagram of the screens is located on page 31 lt 0 gt Cal Soln Value Calibration Solution Value This value relates the value of the calibration standard to a fluorescen
44. n to read last calibration date THE HOME SCREEN READS RFU If ALM is blinking press lt 5 gt and record alarms PHYSICAL INSPECTION 1 15 main power switch on O YES NO 2 Can LCD contrast be adjusted using the UP DOWN arrows ANES aN With Acid Date flowcell was last cleaned With Brush 4 Turn main power to the instrument off and remove enclosure face being careful to remove keypad ribbon cable from the circuit board Tug each wire to the terminal strip to see if it is firmly connected 35 AlgaeWatch REV 1 2 AlgaeWatch TROUBLESHOOTING WORKSHEET AlgaeWatch RECORD SYSTEM VALUES ACCESS KEYS SYSTEM VALUE VALUE lt HOME gt lt 0 gt Cal Soln Value lt gt lt 1 gt Background Value lt HOME gt lt 2 gt High Signal Alarm lt HOME gt lt gt Alarm History lt HOME gt lt gt lt 6 gt lt HOME gt lt gt lt 7 gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt lt 1 gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt 36 AlgaeWatch list alarms AC Cycle Time AC On Time Software Version Raw Blank FS Blank Raw Cal Std FS Cal Std Does AC Relay 8 B go off on when lt ENT gt toggled Oper Hrs Lamp Flow Bl
45. ns are made on this strip see Figure 4 page 37 Electrical connections should be made only by trained personnel AlgaeWatch REV 1 2 APPENDIX vivo Chlorophyll Concepts and Concerns In vivo chlorophyll analysis is the measurement of chlorophyll fluorescence within a living cell The advantage of this type of analysis is that it is quick and simple and does not require special sample preparation or extraction It allows the user to measure 100 s to 1000 s of samples a day in the field However without comparisons to extractive analysis in vivo readings are qualitative in nature Research purposes include e Vertical and horizontal profiling of algal biomass e Algae management for Drinking water facilities Fisheries and aquaculture e Environmental impact and long term monitoring programs e Mapping and tracking algal blooms e Mixing and upwelling in natural waters For questions such as How are the algal populations distributed in the water column Is there an algal bloom developing Where is the bloom Is the algal population increasing or decreasing over time What are the effects of pollution on a natural event on the ecosystem Variation The following biological and environmental issues affect in vivo results 1 42 Health of the organism Healthy phytoplankton will fluoresce LESS per unit chlorophyll than a dying cell Light history of the organism Cells recently in bright light condition
46. oad data logger before changing Date 0 New Changing Calibration Solution Value From the HOME screen Nit s Cal Solu Value 100 Please input ID to toggle ENT XXXX 7 AC Limit Time 0 lt gt to toggle ENT ESC 33 AlgaeWatch REV 1 2 Screens Flow Chart Con t Calibration Screens Clean the flowcell before calibrating Press See Screen Key HOME XXX AlgaeWatch ENT 1 Calib 2 Data Cal XX Days Ago DEC 2 Blank XXX std 1 PleaseinputID ENT CALIBRATE SYSTEM lt 1 gt To Start 1 BLANK SOLUTION ENT BLANK XX 0 BLANK XX wait continue Press lt ENT gt ENT CALIBRATION SOLUTION ENT CAL SOLUTION XX CAL SOLUTION 95 wait continue Press lt ENT gt ENT Press lt 1 gt to End Calibration 34 1 From the HOME screen press lt ENT gt 2 Press lt 2 gt to view raw data output for blank and standard as set during current calibration OR press lt 1 gt to begin calibration sequence 3 If requested key in ID and press lt ENT gt If not requested go to step 4 4 Press lt 1 gt to begin 5 Close the valve to stop sample flow by moving lever to the horizontal position Inject 120 of blank solution flush with 60 ml then allow portion of second 60 ml to remain in flowcell Press lt ENT gt 6 If the number
47. om other systems that are affected or used to monitor algae For example filter run times in a water treatment facility may be negatively correlated with algal biomass By comparing the data you will find that a filter run time may be negatively affected when the fluorescence signal reaches a specific level Once this relationship has been determined you can set alarms on the AlgaeWatch see Alarms pg 18 or through the external logging system to notify you when this level is reached With this information you will be able to take actions to avoid decreased filter run times Other examples of data that can be used to compare against the AlgaeWatch data include presence of taste and odor in water cell counts turbidity or extracted chlorophyll data Once relationships between chlorophyll fluorescence and other measures of interest have been established the fluorescence data can be used to assist in decision making Examples of how the fluorescence data can be used include 1 Location of water intake to minimize treating water with high algal biomass 2 Determining when to treat water for high concentrations of algae and thus minimizing the amount of treatment chemical used REV 1 2 SPECIFICATIONS Power 90 250 VAC 50 60 Hz 5 amps Relay 90 250VAC 50 60Hz 5 amps fused at 3 15 amps Type F Signal Output One 4 20 mA isolated Dimensions 8 W x 4 D x 15 Weight 5 Ibs 2 3 kg Enclosure Appr
48. oximates NEMA 4X Maximum Water Pressure 100 psi Inlet Pipe Size 1 4 NPT male Outlet Pipe Size 14 NPT female Ambient Temperature 40 120 F 4 49 C Maximum Sample Temperature 140 F 60 Relative Humidity 0 100 Overvoltage Category II Pollution Category INSTALLATION Pre Installation Installation A pre installation installation checklist provides important guidelines and information to aid in preparing for installation The checklist is found in Appendix B Power amp Utilities Required Power 100 130 VAC 200 250VAC 50 60 Hz 5 amps Signal Output One 4 20 mA signal isolated Water Sample Supplied to unit at 0 5 gpm minimum and less than 100 psi Drain Sample outlet from the AlgaeWatch should be piped to drain with back pressure drain pipes must be below the unit In installations where this is not feasible contact Turner Designs Technical Support for further assistance Required Tools and Accessories Standard plumbing and electrical tools are required for the installation A terminal strip screwdriver is provided for making terminal strip connections CAUTION The AlgaeWatch should only be used with PVC plumbing kit P N 6500 955 as supplied algaewatch The AlgaeWatch should be piped as shown in Figures 3 and 4 pages 36 and 37 The components may be purchased separately or as a package through Turner Designs Location of AlgaeWatch and Sampling Point
49. r Parameters The unit s data logger is accessed from the HOME screen by pressing the data disk lt gt symbol on the keypad From the data logger menu you can turn the data logger on off set the interval and download and erase data The clock is important to the data logging functions Once the date and time are set and data has been logged download the current data before changing the date or time or you may corrupt or erase your data From the HOME screen press lt gt to see the data logger menu Before entering changing the data logger the unit will prompt for the User Default 8520 and the screen will read Please input ID After entering the User ID the screen will read Datalogger lt 0 gt lt 3 gt log data stop logging data press lt 0 gt Status Stop lt ENT gt to toggle Press lt ENT gt to toggle from Stop to Logging Press lt ESC gt to return to the main Datalogger screen 3 To set data logging interval press lt 1 gt Press lt gt to toggle from 1 2 3 5 10 20 or 30 minutes or 1 second Interval 1 min to toggle 13 algaewatch D Download current data before changing the data logging interval or you may corrupt your data 4 To download the data to a Windows based PC or to erase data from the data logger menu press 2 or 3 respectively Installing the Internal Data Logger Software The In
50. reen press lt 3 gt to enter change the fourth System Value 4 mA Output 3 4 mA Output The 4 20 mA output can be connected by a signal wire to a data logger or other device to collect and store remotely from the unit Outputs can be set to correspond to a certain range of fluorescence values Typically the 4 mA output is set at 0 The unit will reject as INVALID INPUT the new 4 mA value if it is not lower than the 20 mA output value Press lt 3 gt Press lt ENT gt Key in the 4 mA output Press lt ENT gt Press lt gt From the HOME screen press lt 4 gt to enter change the fifth System Value 20 mA Output lt 4 gt 20 mA Output The 4 20 mA output can be connected by a signal wire to a data logger or other device to collect and store remotely from the unit Outputs can be set to correspond to a certain range of fluorescence values The 20 mA output should be set to a value slightly higher than the highest fluorescence signal algal biomass you expect to experience If the signal exceeds the assigned 20 mA value the unit will simply output 20mA If the 20mA value is set excessively high you will limit the resolution of your analog data The unit will reject as INVALID INPUT the new 20 mA value if it is not higher than the 4 mA output value Press lt 4 gt Press lt ENT gt Key in the 20 mA output Press lt ENT gt Press lt HOME gt REV 1 2 The narrower the range of the 4 20
51. rom the unit s light detector This is the output AlgaeWatch uses in conjunction with the Cal Soln Value Background value etc to arrive at the fluorescence readout on the HOME screen It can be used to diagnose problems with the unit For example if the HOME screen always reads zero and the Raw reading is also zero there may be an optics problem If the HOME screen reads zero but the Raw reading does not read zero then check the Cal Soln Value to make sure the proper value is entered FS Acts like an analog meter Indicates the raw signal output as a percentage of the maximum that can be read Value Range RAW 0 00 to 1000 00 reading gt 1000 00 will display OVER FS Oto 100 if Blank equals 0 20 algaewatch REV 1 2 For definitions ranges and default values 3 From the above screen press ENT of these items see Calibration Data Screen page 12 1 Test RlyA ON 2 Test RlyB OFF AC Outputs Two independent solid state relays control AC electrical output through connections terminal strip connections 4 9 AlgaeWatch is connected to an automated valve or other control device via terminal strip connections 4 9 this function allows you to test whether the unit s internal circuitry is operating and terminal strip connections are correct To test the device control of the AlgaeWatch the device itself must be on working and properly connected to the terminal strip Press lt 1 gt or lt 2 gt from
52. s will fluoresce LESS per unit chlorophyll than those that are dark adapted Morphology of the cell Composition and shape of the surrounding cellular material can interfere with the fluorescent signal Light adaptation of the cell The amount of chlorophyll per cell can vary due to its light environment Turbidity Can cause scattering or shading effects depending upon the chemical composition of the turbidity AlgaeWatch REV 1 2 Basic Calibration Concepts 1 Collect an average representative water sample from the body of water to be studied 2 Blank the instrument The best blank would be from the same water sample as the standard but filtered through a 0 45 um filter to remove the phytoplankton DI water or artificial seawater can be fine substitutes if filtered sample water is not available 3 Calibrate your fluorometer with this sample giving it a relative value Ex 50 RFU relative fluorescence units 4 Read all other samples for relative increases or decreases in signal strength To minimize light history effects taking sample readings at approximately the same time each day is recommended preferably at dawn Quantitative Analysis Using periodic extractive chl a analysis in conjunction with vivo analysis can provide more quantitative results when relative information is not enough 1 Calibrate the instrument as discussed above 2 Proceed with study filtering periodic in vivo samples for later extr
53. screen 4 Open the inlet valve to the basket strainer 5 Wait for the unit to equilibrate and air to purge from the unit Calibrating Calibration should be checked regularly Typically calibration should be checked using the secondary standard included in 16 algaewatch the calibration kit every two to three weeks Cleaning the Flowcell How often the flowcell should be cleaned depends on the quality of the water being monitored The flowcell is unlikely to clog but occasionally residue or biofilms can build up on the inside of the glass cuvette A fouled or discolored flowcell can result in low or erratic readings Initially routine flowcell cleaning should be conducted and fluorescence readings before and after cleaning should carefully noted If a significant change in the reading resulted from cleaning a shorter cleaning interval should be implemented If there was no significant change in the reading after cleaning a longer cleaning interval can be used For routine cleaning follow steps 1 and 2 in the Calibration Procedure page 10 open the flowcell shut off valve when cleaning completed For cleaning the flowcell with a brush perform the following steps refer to Figure 2 9 1 Turn OFF the Main Power Switch 2 Shut off the flow to the flowcell Flow is off when valve handle below the flowcell is horizontal It is recommended that the inlet valve be closed and the 3 way outlet valve also b
54. tal Blank and Dilute Acid Solutions Both the Blank and Acid Solutions are not included with the AlgaeWatch System Deinoized water should be used as the Blank 10 Sulfuric Acid recommended or 1 1 HCl is acceptable if the flowcell is well rinsed Calibration Solution Confirm that the correct Calibration Solution is entered before beginning the Calibration Procedure by pressing 0 from the HOME screen see Start Up Procedure for details pg 5 Clean the unit by injecting the dilute acid solution see Notes on Calibration for details page 12 with a syringe into the flowcell The syringe screws onto the Luer Lock fitting on the inlet of the flowcell see Figure 2 page 9 After filling the syringe with 60 ml of dilute acid screw the syringe onto the fitting inject the dilute acid at a slow steady rate into the 10 algaewatch flowcell and allow it to stand for 3 to 5 minutes Next using a clean 60 ml syringe flush the flowcell thoroughly with 60 ml of blank solution Syringes are provided in the Accessory and Calibration Kits To purchase additional syringes see Replacement Parts page 17 Step Press ENT from the HOME screen 1 Calib 2 Data Cal 0 Days Ago Step 4 Press 1 the unit will request ID entry unless recently entered Please input ID If requested enter valid USER ID originally 8520 on the keypad Step 5 Press ENT CALIBRATE SYSTEM 1 to start Step6 Press 1
55. tative maintenance is critical to the success of the AlgaeWatch System Once the unit is installed started up and calibrated the initial settings should not require change Refer to Start up page 5 of this manual for instructions Any start up or shutdown must be made using the AlgaeWatch Main Power Switch see Figure 2 page 9 The System Values are retained in battery back up memory for up to five years However the Start up procedure should be followed to ensure fluorometer calibration and alarm settings are correct if the unit has been disconnected for any length of time This unit has been assembled with a new desiccant plug to ensure the area surrounding the flowcell is free of any moisture As this plug absorbs condensate it will change from a light blue to light pink at the saturation point and should be replaced promptly The unit can remain in operation during this replacement Readings should be monitored during this replacement time as small variances might occur Refer to the Replacement Parts section pg 17 for replacement plugs ordering information The following preventative maintenance should be performed to ensure optimum operation and maximum life Cleaning the Basket Strainer The basket strainer screen should be cleaned as needed as follows 1 Shut off the inlet valve to the basket strainer 2 Remove the screen by unscrewing the clear plastic basket housing and clean the screen 3 Replace the
56. ternal Data Logger IDL software is designed to interface from the AlgaeWatch to a Windows 95 2000 based PC The IDL program is used to download the compressed data from the AlgaeWatch and convert it to an ASCII format for use with a spreadsheet or other program To install the IDL software 1 Insert the IDL disk into your computer 2 Access Run from Windows a setup Be sure to enter the correct drive for the disk Running the Internal Data Logger Software To download data from the AlgaeWatch 1 Using the cable provided connect your computer to the unit s serial port phone jack style connector See Figure 2 page 9 to locate 2 Load the IDL program on the PC by clicking twice on the IDL exe icon The IDL Main Menu will appear 3 Click on Serial Port Setup to select the appropriate port 1 2 or 3 for your PC 4 Click on Download Data from Instrument to File to display the downloading box on the PC REV 1 2 You may download and convert to an ASCII file in a single process by clicking on Download and Convert Data from Instrument to File In this case IDL will prompt you for downloading then conversion in a single process incorporating steps 5 10 5 14 From the AlgaeWatch the data logger to Stop by pressing lt gt from the HOME screen then 0 then lt ENT gt to toggle From the AlgaeWatch access the downloading screen by pressing lt 2 gt
57. what file you would like to convert to ASCII Click on Browse to locate the file or click on OK to accept the default file 10 Click on OK to begin conversion IDL will display Conversion has started IDL will convert the BIN file to an ASCII file of the same name with the extension PRN When Conversion completed appears click on OK to return to IDL Main Menu 11 To exit IDL click on the X in the upper right hand corner of the software window or select Exit from the file menu 12 Disconnect the computer from the unit Erase the data currently in the AlgaeWatch by pressing lt gt from the HOME screen then 3 from the data logger menu Erase data 9 5X to start When data is erased the unit will display Erase data All Data Erased REV 1 2 13 Enter new internal data logging parameters on the AlgaeWatch if desired or resume logging with the previous parameters 14 Return the unit to normal operation Examining the Downloaded Data The ASCII format PRN files can be opened viewed or printed using most standard computer programs To examine the data run your program then open or import the file containing the downloaded data A typical line of data from the internal data logger will look like this your numbers will vary 00001 10 24 91 14 10 28 11 300 i Sample Index Date Time Reading 15 algaewatch REV 1 2 PREVENTATIVE MAINTENANCE Proper preven
58. y To continue Press ENT Remove the syringe and press ENT Press 1 to End Calibration Press 1 to accept the calibration settings The calibration is now complete YOU MUST press 1 or the calibration will revert to the previous settings Step 11 Open the flowcell shut off valve The valve is open when the handle is vertical Calibration is finished Press HOME to return to the HOME Screen and normal operations Notes on Calibrating Calibrate the unit when you have time to go through all the steps without interruption approximately 10 minutes the keypad is not used for 15 minutes the unit will automatically return to the HOME screen The previous calibration will be maintained 2 The AC Relay activation schedule is not altered during calibration 3 Arequest to begin calibration when an alarm is activated will be denied unless the alarm is for High Signal Alarm S H 4 The following alarms will not be monitored during calibration High Signal Alarm 5 No Sample Flow Alarm N F 5 If an internal unit function alarm F A alarm occurs during calibration when you return to the HOME screen ALM will be blinking in the upper left hand corner of the screen Correct the condition causing the alarm then recalibrate the unit 6 During calibration the 4 20 mA output will send out a 4 mA signal 7 Use distilled or de ionized water

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