BioSafety - St. Louis Community College
Contents
1. 18 Wall Hooks for Gowns Exit Door STLOC CPLSMoriann 12 11 2013 25 Biosafety Hood BRDG Lanconco Protector Series Speciation Hot Link to Labconco Hood Manual pdf BabGrar Ne e7souocsae 4 45 Alternate Air Usage Static Pressure 100 With sash ful pen 730 at 0 17 H2O statie pressure With sash open 18 igh Exhausts 470 at 0 07 20 stai pressure Blower Requirements Remote bower required conformance ANSI 29 5 ASHRAE 110 ASTMEBS C22 2 NFPA AS SEFA 1 UL Electrical Duplexes 1 Electrical Standard Domestic Lighting fuorescent Service Fixtures 2 Tested 123 09 by Senco Services Co 701 Emerson Rd Suite 220 63141 Doug Smith 314 432 5100 120 FPM nominal labeled 111 113E 113W 1065515 LOCATED ON ROOF AND SERVES 1 FLOOR FUME HOODS MOUNT SENSOR IN EXHAUST FAN VFO amp PIPE LOW SIDE CONNECTION TO WHERE DUCT Hoods have internal blower Root mounted fan provides exhaust for all hoods Note Atter power lallre these may need resetting by Johnson Controls do max values Roof Mounted Fans Wexford 1 EA FROM Duo SPACE 7 Z w STLCC CPLSMorison 12 11 2013 Biosafety Hood BRDG Lanconco Controls Alarm Settings 1 Press and hold ENTER f2. may contain compet andlor legally privileged informati If vou are mat the intended recipient of thi erage ori ths message has been yoa error please immediately ulert the vender by reply e mail und delete this message and any are not the ended recipiens ie coping storage of this mensage or prohibited Biosafety Guidelines Biosafety in Microbiological and Biomedical Labs NIH USHS Biosafety in Microbiological and Biomedical Laboratories U S Department of Health and Human Services Public Health Service Centers for Disease Control and Prevention and National Instimtes of Health Fifth Edition 2007 U S Goverment Printing Offise Washington 2007 Hot link to Biosafety in Microbiological and Biomedical Labs pdf rcr BP Biosafety Regulations National 5 OSHA Regulations Standards 29 CFR National Research Council Recommendations Concerning Chemical Hygiene in Laboratories Non Mandatory 1910 1450 App Regulations Standards 29 CFR Table of Contents Part Number 1910 Part Tile Occupational Safely and Health Standards Subpart Z Subpart Toxic and Hazardous Substances Standard Number 1910 1450 App A Tile National Research Council Recommendations Concerning Chemical Hygiene in Laboratories Non Ma
3. Hot Link to United States Dept of Labor OSHA Formaldehyde Regulation Page html STLCC CPLSMorison 12 11 2013 Page 6s Biosafety Formaldehyde Meter Monitor example only presently not at FV www ppm technology com Formaldemeter 400 The Formaldemeter 400 offers a simple way to monitor levis of iore tra workplace The instument has been designed wih ease of use n mind being operated via simple four bution keypad and featuring an easy to read four digit display The Formaldemeter 400 uses a welt proven electrochemical formaldehyde sensor that wil Hovde an immediate indication atmospheric levels Unixe omer formaldehyde manitoring devices such as colour stain tubes and badges Formaidemeter 400 capable of measuring many samples consecutveiy without the need or inconvenient ancilary equipment Realtime results also mean that Guerexposuros can be identifed 2s they occur without the delay associated wih laboratory TECHNICAL SPECIFICATION Sonsor Electrochemical Calibration Infield formaldehyde calibration standard Display 4 digit LCD Sampling Frequency 0 3 minutes depending on previous reac Sample volume Approx 10 mi Detection Range 005 10 ppm other ranges availabie on Precision 10 at 2 ppm leval Response Time Approximately 8 seconds from sampling Power
4. V LihtButtou Tun he UV lap on or off When Be UV Saar Son mede esu miss De laren When the raed be it rams off sent Timer Button Allows yov ro selest cies repeating 77 or an elapsed mer Mines all ausible alarmis for sae natem er When mode tais unn select a pon Men Bolton is boton toggles be display bermeen display ad modes Wien the men mod ds ba rers yo fo the emis men level Select Buttons allow you to choose ditfereat options The menn mode BioSafety Cabinet BRDG VWR LabConco 6ft Type 2 These glacier white biosafety cabinets feature a unique interior mounted line of sight LCD information center that displays icons for at a glance monitoring of activities including filler loading disruption in airflow and cabinet components in use A bar graph displays percentage of remaining HEPA filter A status line gives specific feedback on alarm conditions such as when the sash is too high An alert warns when the filter life diminishes to 20 or when there is an airflow change such as an inlet grille or exhaust outlet obstruction Cabinets are constructed with a durable epoxy coated steel exterior and a stainless steel liner with removable stamped one piece dished work surface A curved stainless steel inlet grille gently supports the user s forearms
5. i tht ped dough ete or wok with ok aa Tica a tha gui dar Sane tenet Dow appr tie ev grat atrial exci adr ater doces Logic Das IL Type Biosafety Cabinets ze despe to Seca rond iter ar Say tn ugh the eps Convection Wen meti eae my to ined whe pouty Taf spy HEPA eco ek cet wr oft sant a pose atr ile thee nr ua and tracer as moving wenns vu EAT CfyfjMerson Ie HD e 469 Velocimeter Check Air Flow in Biocabinets Hoods example only not presently at FV 8515 and 9525 models are an economical og Air Meler compromising accray or reson Professionals ind them the elo for a measurements spray baci or Tor IAQ and system checks The Model 9515 features a 40 inch 101 6 cm telescoping integrated probe for dificult to reach measurements The Model 9525 has a 3 nch retractable probe and is UL listed as intrinsically safe for use in hazardous locations for Divisions 1 and 2 in Class Groups C and D Class ll Groups F and G and Class Velocity Rango
6. standard fat blue color with a smooth surface for of cleaning Ordering Information Cabinets are also STLCC CPLS Momson 12112013 Pane ss Biosafety Waste Container Acrylic BRDG Large Waste Container 76 8 x 44 5 x 438 30 177 177 89030 140 Each 490 22 These waste containers are Ideal for keeping sharp objects and liquid waste contained in the event of waste leakage Clear acrylic casing permis visibility of warning insignias or biohazard bags Each container has a hinged 16 for convenient placement of waste materials Constructed of clear tem 7 thick acrylic BRDG Park BioHazard Container Protocol per BS 3 10 11 1 empty red biohazard storage box from under the hoods in Molecular lab and line it a new regular thickness bag 2 Bring storage box to waste container and transfer entire biohazard waste bag into the box bag This will double wrap the biohazard in the storage box 3 Return red biohazard storage box to the area under the hoods until the next scheduled waste pickup 4 Place new heavy duty Biohazard bag in the waste container Page st Morison 12 11 2013 Biosafety Glass Disposal Containers BRDG Floor Disposal Carton 30 5 x 30 5 x 68 5 12 x 12x 27 56617 801 Pack of 6 5368 Disposable cardboard receptacles with 2 thick polypropylene liner safely contain broken glass and spilled liquids Entire unit may be disposed o
7. Gowning Room H Chem Spill Kit BlosafetyCabinet Fume Hood ER Wash amp Drench gt remi 1 Gas Sensorialarm First Ald Kit Desiccator drybox Cabinet Poly BioSafety Guidelines Fume Hoods Cabinets Wash amp Shower Storage Spill Gas Detection First Aid Kits Fire Ext Detect Instrument Picture to Jump to that section of SOP 9 BondingClips Flammables Cabinet Heat Gloves Ethidum Bromide STICC CPLSMorson 12114 Unit Biosafety BioHazard Waste Pickup per Becky Staerk July 2013 Medi Sure 877 963 3277 x325 Customer number 1239 Ask for Glenda and remind her we are at the BRDG Park location 1005 N Warson BioHaz Service Jennifer Houston 660 882 2939 jhouston medserve com www medserve com Bstaerk Note It may take multiple calls to get a confirmed pickup date time and that may need to be rescheduled Biosafety BioHazard Waste Pickup per Becky Staerk Nov 2012 Marcy Cline set this up for us but our Biohazard is through Med Assure Nov 2012 Med Assure 913 364 3000 or 913 364 3004 ext 305 Ask for Annette STLCC CPLSMomaon 12 11 2013 Pages Biosafety Special Use Permit Status Marcy Cline Aug 2012 From Cline Marcy J Sent Wednesday August 08 2012 2 35 PM To Boedeker Elizabeth D Subject Re Special Use Permit We not have nor are required to have
8. 4 STCO CPLSMorison 12112013 Biosafety Cabinet Latest Certifications old FV cabinet 21222 We July 2011 sf EHEE 8 Biosafety Cabinet Service Recertification Work Order 2012 STLCO CPLSMorison 12112013 Page 39 BioSafety Cabinet Labconco Class 2 BRDG 6 Purifier Digital Delta Series Class Il Type A2 no external exhaust requirements Biological Safety Cabinet Catalog Number 36213039728 990738392 SILCO 009227 Product Specifications Dimensions 78 x 31 6 d 62278 Measured 74 Y inches RGM Electrical Specification 110 volts 50 Hz 7 amps International oduct Subcategory Class Il Type A2 Biological Safety Cab Nominal Width 6 Electrical Requirements International Sash Opening Exhaust Volume 403 483 CFM Lighting Fluorescent Ultraviolet Compliance CE Built In Options UV Light 2 service fixtures Supply HEPA Filter for 6 Purifier Logic Catalog Number 3838403 List Price 5890 00 HEPA Filters are 99 99 efficient with particles 0 3 micron and have aluminum frames with either aluminum separators or separatorless pleats Notes of Maintenance Certification from conversation with Naumann 10 14 09 1 ALFV we use ACE Electrical Lab Systems 1550 S Kingshighway 6
9. container unless it has a valve that cloned and can prevents material ran Spilling when tumed over This is important ve sen funnels eft drums thar would be a finding Drums withthe rng left loose would be Close thers up Flammable liquids in drums should be grounded Make sure the grounds are connected AN Non Hazandous waste must be labeled with the proper label Used Od Used Od filers other suc as Latex Paint musthave the green laste S Used uorscen balbs must be in containers that are closed with dte purple 6 Rechargeable batteries must have terminals taped or bagged and segregated by type Only ane type per container e separate om the Litiun e 7 Stores area should be designated with the SSA sign provided earlier IF you don t have one let me know and get it Masked remember we pick up hazardous waste every 180 days at the end af spring and semesters and as needed beeen is motan exhaustive Hst As you check your SAA imagine the questions they might ask Ifyou have spill stains how will you explain Givemea calland we ll work on i Benter me than EPA Heath and Spelt Rak Management St Louis Community College Cosand Center 3005 Broadway Se Lous MO 63102 2800 Phone 314 539 5127 Fax 314 539 5453 mal meine 8ste edu E MAIL CONFIDENTIALITY NOTICE The contents of tide e mait and any attachments ure intended solely fur the
10. is 80 full 4 Verity open flow rato from both eye sprays with clear stream MOP and CLEAN area and place yellow placard safety until area is fully dry SIGN and DATE Inspection Log Sheet Page Biosafety Emergency Shower Eye Inspection Log Location Room Date d m y Inspected by Printname Initials Notes Page LS Morison 12 11 2013 BioSafety Eye Wash and Drench Shower Item Number Manufacturer Guardian Equipment Hot Link to Guardian Ansi Safety Guidelines pdf STLCC CPLSMorison 12 11 2013 Page STiCC CPLSMorison t BioSafety Se Cur All Cabinet Flammable Safe 4 Se Cur All Cabinet A145 45 Gal capacity 9 5 4 Storage cabinets shall not be required by this code to be ventilated for fire protection purposes 9 5 4 1 If not ventilated storage cabinet vent openings shall be scaled with the bungs supplied with the cabinet with bungs specified by the cabinet manufacturer 9 5 4 2 If ventilated for any reason the storage cabinet vent openings shall be ducted directly to outdoors in such a man ner that will not compromise the specified performance of the cabinet and in a manner that is acceptable to the authority having jurisdiction NFPA code 30 Current as of Jan 2009 22 Biosafety Flammables Cabinet BRDG Secur ALL 4ft Cabinets are constructed of tough double walled 18 gauge galvanized stool with 4 8cm 11 27 alr space be
11. Glare free fluorescent lighting is located outside the contaminated work area Cabinets also feature a fully closing clear 6 4mm 1 47 thick tempered safety glass sash with two small sculpted sash handles a counterbalanced anti racking mechanism and a 10 slope An electrical duplex receptacle with ground fault interruption and stainless steel splash cover is located on each side of the cabinet Electronic security lock with optional activation requires a code to operate the cabinet Supply and exhaust HEPA filters are 99 99 efficient Purifier Logic Class Biosafety Cabinets Labeonco Sash Open Voltage Services Exh Vol cfm Labconco VWR 20 3 8 115V 60Hz 2 402 442 3460809 97000858 Hot Link to Labconco Biosafety Cabinet Brochure pdf Hot Link to Labconco Biosafety Cabinet Manual pdf Hot Link to Labconco BSC Technical Specs and Certification Manual SSTLCO CPLSMomaon 12 11 2013 Page at Biosafety Cabinet Logic Parts amp Circuit Breakers is ole TS Eem UV Lamp 4ft PN 9721900 Appendix A pg 51 of Labconco Manual UV Lamp Kit w ballast PN 3858500 Appendix pg 57 of Manual CPLS Morison 12111 2013 Pages Biosafety Logic new Cabinet Latest Cert July 2011 STLCC CPLSMorison 12112013 Biosafety Labconco Recertification Service Aug 2013 oom pamm
12. 5 7 TI 3 aia l EA EIE AM 09 oh mentite ned r9 RE pM 95 Sa ena go a Pc mu SENS Amin m pp p duco MESS E een TE P e 8 Prick Biosaftey Inspection Guidelines From Cine Marcy 3 Sent Thursday January 27 2011 1 17 PM from Macy Cline 1 27 11 EPA Hazardous Waste inspector drop in on local company yesterday at 3 00 pn Said was in the area and wanted io take eir hazardous waste storage This isa good opportuni for uso check ourselves so revised the Inspection Protocol list Please review it along withthe following We operate under the Salle Accumulation Areas SAA rules which are es rigorous than the full Hazardous Waste Rules We need to make sure we can comply with tbese rules Please check your SAA for the following Lo AU hazardous wastes are labeled with the words Hazardous Waste af the materia in the container dute the first drop went in the container Label isin gool condition and legible 1 All container af hazardous waste must be closed except when adding material The rule if the container were knocked over would materials spil I vs is naa lowed container You cannot eave Tunnel
13. SV PP3 alkaline battery Enclosure ABS plastic Weight Instrument 2709 Complete 750 g Dimensions _Insinument 150x80x34 mm Carrying Case 266230 60 mm Kit Contents 1 Formaldemeter 400 instrument with battery 1 Formaldehyde calibration standard tube 1 Pack of phenol fiters 10 1 Thermometer 1 Ball point Operation manual ACCESSORIES 2 mne o Biosafety 3M Formaldehyde Monitor 3720 Tab Example only presently not at FV note must be mailed to lab for analysis Important Note Prepaid analysis address is B C Chemistry Department 11001 Hampshire Avo S MN 55438 The 3M Formaldehyde Monitor 3720 with Prepaid Analysis uses the proven scienti principle of diffusion which Is the gradual mixing of molecules of two more substances It monitors personnel or work areas health laboratories and in the chemical foundry and textile industries Analysis procedure is available upon request The monitor uses a bisulto impregnated fiter and is analyzed by colorimetry 1 be used for 8 hour TWA sampling to meet the OSHA requirement of i 25 accuracy at 0 5 5 0ppm However this monitor does not have the sensitivity for short term exposure limit STEL monitoring Ithas an 18 she 3M 10 70 0700 9394 6 STiCC CPLSMorison 12 11 2013 Page STCC CPLSMorison
14. doing that Push the RED emergency gas off button at the lab door exits Use fire extinguisher if it seems reasonable and only if there is a clear exit behind you Fire extinguishers Located in each lab room near yellow box First Aid kits Located in the main corridors at Break Room and Conf Room PASS operating instructions P Pull the ring pin out with a strong force Aim the hose at the lower base of the fire 5 Squeeze the lever handle 5 Sweep the hose side to side until flames are extinguished Inspect extinguishers once per month to verify green state Notify all new employees interns and students about fire safety STLCC CPLSMorison 12 11 2013 Biosafety Fire Safety Drill Gathering Location Let s designate this island labeled with orange STAR lt the STLCC meeting location in case of emergency such as the recent fire drill Upon exiting the building all faculty and staff should check in al this location Faculty should be monitoring the exit of their students if classes are in session CBRE Tamous este Pan Sty Please note that currently building 2 is our close parking lot and building 3716 also currently parking lot We selected a location that would not change over the next few years as the landscape SK lt Biosafety Handling Flammables OS
15. exposures is needed should be a regular continuing effort not merely a standby or short term activity 6 11 Its recommendations should be followed in academic teaching laboratories as well as by full time laboratory workers 13 5 Observe the PELs TLVs The Permissible Exposure Limits of OSHA and the Threshold Limit Values of the American Conference of Governmental Industrial Hygienists should not be exceeded 13 STLCC CPLSMorison 12 11 2013 232 Biosafety Shower and Eye Wash Testing Requirements ANSI Standards ensure that your eyewash stations and showers are always ready when you need them it is important that the requirements for test procedures and maintenance set forth in 2368 1 2004 are followed The American National Standard for eyewashes and showers provides minimum requirements for these units to function properly The requirements for testing and maintaining any of the eye eye face washes and showers is based the manufacturer s instructions and ANSI requirements Generally the manufacturer s instructions state that the units should be inspected tested and recorded weekly Individual owner manuals should be looked at for the specific manufacturer s guidelines The ANSI standard 2358 1 5 5 2 states that plumbed eyewashes washes and Showers should be activated weekly for a period long enough to verify operation and ensure that the flushing fluid is available While activatin
16. i If you discover a chemical spill 1 Immediately inform all in the area to leave the area 2 If the chemical comes in contact with any person Remove all contaminated clothing If there is contact with a person s eyes rinse eyes with water for at least 15 minutes If there is contact with a person s skin thoroughly rinse under water and then wash with soap and water 3 Call Campus Police 4300 4 Inform Campus Police of the type and amount of chemical spilled Do not clean up any chemical spill on your own without permission of Campus Police Hot link to STLCC FV Emergency Response Guide 3 09 pdf ee e oreen pra BioSafety Safety Symbols NFPA and HMIS EQUIPMENT Biosafety Fire Extinguisher Lab Class ABC Dry Chemical Dry chemical extinguishers aro useful fr either class ABC or class BC fires check tho label and are your best around choice for common fre situations They have an advantage over CO2 and clean agent extinguishers in that thay leave a blanket non flammable material on the 4 extinguished material which reduces the Ikeliaod of reigntion They also a terrible mess but if the choice is a fie or a mass take the mess Noto that there are two kinds of dry chemical extinguishers Tyne BC fire extin
17. plastic resistant to staining crazing and chemical attack and have built in protection that blocks 99 of UV light Desiccant can be placed under the bottom shelf or on any shelf A hygrometer dial in the door allows for easy monitoring of relative humidity Carrying handles make them easy to relocate Amber cabinets reduce visible light penetration by more than 50 and blocks 99 of UV light Has two perforated shelves that are removable Door has two latches at the top and loops for lock or tamper evident seal Can be stacked three high STLCC CPLSMorison 12 11 2013 BioSafety Liquid Nitrogen Storage Xfer Tank LINDE LAB DEWAR LINDE UNION CARBIDE Model 18 31 SN 230 126 M1 30 Liter Max N Capacity LS Morison 12 11 2013 Biosafety Gas Tank Cylinder Storage 5 Shall not be stored in exits egress routes Shall be stored within well ventilated area Shall not bo stored in damp areas near salt corrosive chemicals fumes heat or where exposed the weather Shall bo stored in an upright position Shall be secured with a chain or appropriate belt above the midpoint but below the shoulder Laboratory cylinders lass than 18 tall may be secured by approved Stands or wall brackets Shall be capped when not in use or attached to system i the cylinder will accept a Shall be kept at least 20 ft away from al flammable combustible or incompatible subst
18. 12112013 BioSafety Other Notes Future home of other or more details protocols STiCC CPLSMorion 12 11 2013
19. 3110 314 771 7272 to do annual cleaning filler changes and Certification processes a 2 3 day operation that involves use of formaldehyde and potentially other agents in the cleaning Typical cost is 5 per device 2 For questions of air velocity CAV we call FV HVAC personnel to get readings SSTLCO CPLSMomaon 12 11 2013 Pags 40 Biosafety Labconco 2 Cabinet Startup Protocol Tum off UV light if included 4 Slowly raise the sash the bottom of the sash aligns with the sash indicator decal located on the left side of the work area 4 Tum on the fluorescent light and cabinet blower if the Smart Start features have not been activated Check the air grilles for obstructions and note the filter gauge reading Allow the cabinet to operate unobstructed for 5 minutes Wash hands and arms thoroughly with germicidal soap 9 Wear a long sleeved lab coat with knit cuffs and over the cut rubber gloves Use protective eyewear Wear a protective mask if appropriate a ie Biosafety Labconco A2 Cabinet Wipe Down Loading Chapter 5 Using The Cabinet Wipe Down Raise the sash to its full open position approximately 21 75 inches or Mute the alarm by depressing the Alarm Silence switch Wipe down the interior surfaces of the cabinet with 70 ethanol suitable disinfectant and allow to dry Loading Materjals and Equipment load the ma
20. 7060 no data 100 To data 2 no data 10 zuo E 10 E 0 1000 400 03 so 5 1 200 2 5 no data no data so E 70 003 sin 2 1880 no data ox 75 14 0095 262 52 E 2 1 188 1 1000 0 no data 1 50 am 1000 no data am 5 1 200 2 5 200 PEL 200 3 skin 3 EJ 1600 200 15 1800 7 14 250 5 E 750 page 21 BioSafety Gases Concentration Safety Levels Hydrogen Lower Explosive Flammability Limit 4 volume of air Minimum Ignition Energy joules 0 016 28 in pure oxygen 0 0012 per Wikipedia Carbon Dioxide per Wikipedia 1 13 11 Toxicity and its effects increase with the concentration of CO given in volume percent of CO2 in the 4 1 can cause drowsiness with prolonged exposure At 2 itis mildly narcotic and causes increased blood pressure and pulse rate and causes reduced hearing At about 5 it causes stimulation of the respiratory center dizziness confusion and difficulty in breathing accompanied by headache and shortness of breath At about 8 it causes headache sweating dim vision tremor and loss of consciousness after exposure for between five and ten minutes STiCC CPL
21. 8515 0 to 4 000 fumin 0 to 20 m s Rango 9525 0 to 2 000 010 10 mis Accuracy 5 of reading or 55 fumin 20 025 m s whichever is greater Resolution 1 ft min 0 01 m s Temperature 9515 only Range 0 to 200 18 to 93 C Accuracy 4052 40370 Resolution 0 19 014 Instrument temperatura range Operating Electronics 40 to 113 F 510 45 C Model 9515 Operating Probo 0 to 200 f 18 to 93 0 Model 9525 Operating Probo 14 to 140 F 1010 6070 Storage 410 140 2010 60 C Notes of Maintenance Certification trom conversation with G Naumann 10 14 09 1 ALFV we use ACE Electrical Lab Systems 1550 S Kingshighway 63110 314 771 7272 to do annual cleaning changes and processes a 2 3 day operation that involves use of formaldehyde and potentially other agents in the cleaning Typical cost is 5K per device 2 For questions of air velocity ar we call FV HVAC personnel to get readings STLCC CPLSMorison 12 11 2013 Page e Biosafety Emergency Shower and Eye Wash 2 BRDG Guardian GBF2100PCC Recessed Safety Station Polished Chrome Plated Brass Shower Head application Recessed barrer reo wash and shower safely station with ceiling mounted exposed shower head Recessed design conserves valuable floor space while eliminating the clutter and obstruction created by conventional emergency equipment Eye Spray heads 17 IPS brass stay open ba
22. 9 Biosafety Good Lab and Manufacturing Practices Link to FDA Good Laboratory Practice for Non Clinical Labs TITLE 21 FOOD AND DRUGS CHAPTER I FOOD AND DRUG ADMINISTRATION DEPARTMENT OF HEALTH AND HUMAN SERVICES SUBCHAPTER A GENERAL PART 58 STiCC CPLSMorion 12 Page 20 TLVSTEL PEL BioSafety Regulations Toxicity Air Quality Terms Guidelines Chemical etic Acid Acetone Acetonitrile Acrylamide Ammonium hydroxide Benzene CChiorafarm Diethyl ether Ethanol Ethidium Bromide Ethyl acetate Formaldehyde Hexane Hydrochloric Acid Hydrogen peroxide Mercury Methanol Nitric Acid Phenol Pyridine Sodium Hydroxide Sulfuric Acid Teluene Threshold Limit Value Time Weighted Average Average concentration for 8hrs Short Term Exposure Limits Ceiling Exposure Limit Permissible Exposure Limit Flammable chronic acute chronic carcinogen possible carcinogen mutagen mutagen acute chroni acute carcinogen chronic neurotoxin hepatorin chronic chronic irritant Notes FV Equipment DB source table 29 11 Seidman Moore Biotech Manual Yes Yes Yes Yes Yes Yes Yes ves Yes Yes Sate le 15 minutes 4 events per day Must never exceed this amount daily basis requirement legal limit TLV ppm TLV mg m 3 PEL ppm 1o50 oral E E Ez ama 908 ans
23. HA Guidelines Hot link to Flammable and Combustible Liquids pdf Bonding Wire Container to Container 3 FT UNINSUL AN ST WIRE DUAL VWR CAT 8 56620 208 Attach alligator clip end to source and receiving container before opening source Leave attached during pour STLCC CPLSMorison 12 11 2013 Page 11 BioSafety STLCC Risk Mgmt and Other Lab Contacts From Cline Marcy J Sent Wed 10 21 2009 12 57 To Naumann Virginia L FV Bio Mazdra Adrienne FV Chem Gero Susan A MC Lab Tech Bast Janet 0 MC Lab Tech Dil Kimberly MC Lab Chem Tolentino Susan FP Lab Chem Brandon Strawb Rachel R BioSci Lab Tech CC Morrison Robert FV Bio Instrumentation Kroder George T Risk Mgmt Cosand Subject FW Biosafety FV Lab Safety Guidetines Manual Bob Morrison was kind enough to post the existing Laboratory Guidelines on the STLCC web for us The URL for our website is again htlp users stic eduidepartmonts vbio See below for how it looks on the page This may be helpful fo you as you are training your new students and student workers They haven t been updated since 2003 you notice anything that needs to be updated please let me know and we ll make the change 1 haven t had time to thoroughly review it yet but wil in the near futuro Thanks Bob That was a big help STiCC CPLSMorion 12 11 2013 Page 12 Biosafety Lab Safety Guidelines File Storage Structure on Server
24. SMoriaon 12 11 2013 Page 22 Lab Systems Gas Detection Sensor Alarms Oxygen Airgas Builtto Industrial Scientfcs highest quality and reliability standards GasBadge Pro provides a of gas hazard protection with more features than any other single gas monitor available Interchangeable smart sensors enable the GasBadge Pro to be quickly adapted to monitor unsafe levels of oxygen or any one of the following toxic gases carbon monoxide hydrogen sulfide nitrogen dioxide sulfur dioxide chlorine chlorine dioxide phosphine ammonia hydrogen and hydrogen cyanide GasBadge Pro communicates direct an infrared interface optional accessories like the Docking Station Datalink and infrared printer to further simplify and automate calibration function bump testing and data downloading Standard STEL and TWA readings and logging of up to ona year of survey data are featured along with an event logger that records the past 15 alarm events Housed in a rugged enclosure tno monitor is immune to RF water resistant and extremely durable protective concussion proof protects the unit from extreme abuse in a variety of harsh industrial environments simple and intuitive four button navigation allows easy access to setup operation and calibration functions Lifetime warranty Industrial Scientific GasBadge Pro Oxygen Single Gas Monitor 124181000603 363 97 OR Altair Pro Single Gas Detecto
25. ances Storage areas that have noncombustible wall at least 5 ft in height and with fire resistance rating of at least 30 minutes may be used to segregate gases of diferent hazard classes in close proximity to each other Shall be stored that cylinders are used in the order in which thay aro received Shall be stored so that gases with the samo hazard class are stored in the same area Inert gases are compatible with other gases and may be stored together Shall not be stored longer than one year without use Shall bo stored that ful cylinders remain separate from empty cylinders Ames Laboratory Environment Safety Health amp Assurance Copyright August 1997 Hot link to Gas Tank Cylinder Safety Guidelines pdf STLCC CPLSMorison 25 2048 Page st Biosafety Formaldehyde OSHA Requirements htip iWww osha govipls oshaweb owadisp show document p table STANDARDS amp p id 10075 Permissible Exposure Limit PEL 1910 1048 1 TWA total weighed average The employer shall assure that no employee is exposed to an airborne concentration of formaldehyde which exceeds 0 75 parts formaldehyde per million parts of air 0 75 ppm as an 8 hour TWA 1910 1048 c 2 Short Term Exposure Limit STEL The employer shall assure that no employee is exposed to an airborne concentration of formaldehyde which exceeds two parts formaldehyde per million parts of air 2 ppm as a 15 minute STEL
26. any permits for the Brdpk 5 facility All hazardous waste is disposed by Heritage Environmental bio hazardous waste is disposed by Waste Management Neither require a permit Sent from my iPhone STLCC CPLSMomaon 12 11 2013 Biosafety St Louis Community College Laboratory Safety Gi Florissant Valley Campus Implementing the Laboratory Safely Guidelines Biology Chair Guidelines for Safety Biology Chair Student Form for Signature 1 0 Personal Protective Equipment in the Lab 2 0 Laboratory Hazard Communication MSDS and Container Labeling 3 0 Chemical Storage 3 1 Chemical Hygiene 3 2 Purchasing and Use of Chemicals 4 0 Lab Waste Disposal 4 1 Metropolitain Sewer District Ordinance pat 4 2 Hazardous Waste List 4 3 Hazardous Label 4 4 Ethidium Bromide Disposal 5 0 Contral of Bloodborne Pathogens in the Lab 6 0 Response to Emergencies in the Lab 7 0 Lab Inspections 7 1 Lab inspection Form 8 0 Safety Training for Students and Staff Working in the Lab Copyright 2001 St Louis Community College rights reserved Pages Risk Management Department Cosand Center Ext 5120 STLCC CPLSMorion 12 11 2013 Biosafety Regulations Florissant Valley Emergency Response Guide FV3 09 eom Chemical Spill 4
27. d 28 Em 4 Air Shower HEPA loose contaminants Change Room Sand wasting son ouch operator om B 10 7 Dispenser for glove liner or underglove H 8 9 Glove washingidrying station o x 10 Headwear dispenser EET 11 Face mask dispenser x Lun Ke 12 Wastorecaplaco OL o emu 007 13 Garment Storage Cleanroom 13 Open Rack lor reusable garments Bio Cleanroomi Air Leek 8b Enclosed Cabinet for reusable garmer Aseptic Area 14 Waste Receptacle 15 Ergonomic Lean Rails support personnel while donning coveralls 16 Gowning Platform provides a nancontaminated surface to prevent violation of garment 17 Wiper disponser used clean bench prior to puting on booties 18 Bootie shoe cover dispenser may also be incorporated into benches 19 Waste Receptacle 20 Gowning benches used when putting on booties Booties stay on clean side 21 Cleanroom mirror side by side wpostar showing correctly garbed modal 22 glass and overglove dispensers 23 Air shower may bo required in cleanroom entrance 24 Garment hamper for reusable garments to be laundered 25 Waste receptacle for disposable garments 26 Tools supplies cabinet for overstock garments or cleanroom supplies STiCC CPLSMorison 12 11 2013 Pages BioSafety Gowning Room 120 Proposed Layout
28. el found near the unit and insert the funnel in the hole at the top reservoir 2 Slowly pour up to 2 of gel solution through the funnelfilter into the upper reservoir 3 When liquid is all in the upper reservoir discard coffee filter in biohazard disposal bin and rinse funnel for next use 4 Do not remove the cap to the upper 5 Do not connect extra tubing to drip must remain at level of reservoir 6 Replace cartridge about every two years Close ends with yellow caps and incinerate The Elchrom Selenite Company Bind ET Ethidium Removal System removes large quantities ot Ebisu The carnage has ot at least 2 g of Ethidium Ts equ 4 0001 ot elecrophorsis butter ar staining soon at a Soncenraton 0 5 Fiowrate Saturated cartndges can be deposed ot va Incineration Dimensions 335 x 225 47 cm Product 2380 removal system nang cartrage 2351 Replacement care or Incuding camidge SSTLC CPLS Monson 12 11 2013 Pages Biosafety Ethidium Bromide Filter get quote B stiouscommum Googe nens Google C 5 C Panama Cay dean 2 Home About Us What s Naw Promotions Products 1 to your Cate Pagese Mori
29. f as a whole once safety cap is pulled place Available in floor and benchlop models Carton cover with spring actuated bag holder floor model improves sanitation and safety of waste disposal uloclavable prevents inadvertent contact with bag contents and is color coded for easy identification STLCC CPLSMorison 12112013 Pane ss Biosafety Chemical Spill SpillSolv Multikit For safe easy effective treatment in the laboratory SplSolv kis are available for acid caustic solvent formaldehyde or mercury spill Caustics and acids are converted into nonhazardous compounds that meet EPA specifications for nonhazardous waste simplifying cleanup and disposal Solvent can be easly treated and transported with the adsorbent in the solvent pill kit This adsorbent wil also act as a vapor suppressant FOR ALL SPILLS 1 FIRST Put on Safety Glasses and Gloves 2 Send someone else for help to Emergency numbers or get Fire Extinguisher 3 Take Student s to Emergency Shower Eye wash station if splashed with chemicals 4 Determine the chemical type in the spill DON T GUESS 5 Pour granules from the appropriate bottle forming a ring around the spill area diking then proceed toward the center _ FOR CAUSTICIBASE SPILLS USE Caustic Granules 00 mm STLCC CPLS Morison 12 11 2013 Biosafety Ethidium Bromide Removal System 1 Place a fresh coffee filter in the blue funn
30. g plumbed eyewashes eye face washes and showers you should also veril that they are providing luke warm tepid water between 60 100 Self contained eye washes obviously cannot be activated weekly without using up valuable solution so ANSI recommends visually inspecting the unit to see the fluid needs changing or supplementing Plumbed and self contained units shall also be inspected and maintained in accordance with the manufacturer s instructions For eyewashes this includes protecting the nozzles and flushing fluid against airborne contaminants STLCC CPLSMonaon 12 11 2013 Biosafety Links to Safety Videos http vimeo com 1177975 Emergency Response 12 min HHMI Chemical Hazards 9min HHMI http vimeo com 1177949 Chemical Storage Hazards 11min 1178037 Glasswashing Hazards 10 min HHMl http vimeo com 1177999 Centrifugation Hazards 9min http vimeo com 1177927 Videos below are available for checkout at FV VHS format and BRDG DVD see the Respective lab managers for the original LST 101 Orientation LST 102 103 104 Showers Flammables Formaldehyde LST 105 106 107 108 Electrical Ergonomics MSDS Hoods LST 109 110 111 112 Contamination Glassware Emergencies GasCylinders SSTLEO CPLSomaon 1211 2048 Fage 1
31. guishers contain sodium or potassium bicarbonate Type ABC fire extinguishers contain ammonium phosphate Class A fires are ordinary materials like buring paper lumber cardboard plastics etc Class B fires involve flammable or combustible liquids such as gasoline kerosene and common organic solvents used in the laboratory Class C fires involve energized electrical equipment such as appliances switches panel boxes power tools hot plates and stirrers Water can be a dangerous extinguishing medium for class C fires because of the risk of electrical shock unless specialized water mist extinguisher is used Class D fires involve combustible metals such as magnesium titanium potassium and sodium as well as pyrophoric organometallic reagents such alkylithiums Grignards and diethylzinc These materials burn at high temperatures and will react violently with water air and or other chemicals Handle with carel Class K fires are kitchen fires This class was added to the NFPA portable extinguishers Standard 10 in 1998 Kitchen extinguishers installed before June 30 1998 are grandfathered into the standard STiCC CPLSMorison 12 11 2013 Link to OSHA Fire Extinguisher Reqmts m Biosafety Fire Safety Rules at BRDG General Rules if a fire is spotted Pull alarm at North entrance doors to and or notify others to evacuate the building to the North parking lot area Call 911 or ensure another is
32. k to Labconco Purifier Logic Series User Manual pdf Re Certification Call George at ACE 314 974 6616 duly each yea Notes of Maintenance Certification conversation with G Naumann 10 14 09 1 ALFV wo use ACE Electrical Lab Systems 1550 8 Kingshighway 63110 314 771 7272 to do annual cleaning changes and Certification processes a 2 day operation involves use of formaldehyde and potently other agents in the cleaning Typical cost is 5K par device 2 For questions of air velocity or CAV we FV HVAC personnel o get readings 3 HEPA Fiters are 99 90 efficient mth particles 0 3 micron and have aluminum trames wih either aluminum separators separalovess Moving 8 Storage 13390 Lakefront Drive Earth City wes 12 2 63045 Salesperson is Bill Kirchner 314 344 4300 Biosafety Cabinet Controls Logic Purifier BRDG Bloner Baton _ or ps the cabinet blower When Blower 15 m Sem mode losing tess ums theblower of Wien sach raised tie lower resa mode when sows to mscr in the wok tea When the sss reopened blower erae Prewipg bon ove Stunt Str cs pain Light Button the Closing the sad atat aust hts off When legi 5 rais the sadi mene he lt on
33. ll valve with stainless steel panic actuator eyelface wash spray head assembly Two FS Plus spray heads mounted on supply arms Each spray head internal flow control and fiter to remove impurities from water wash valve 1 2 IPS plug type valve with Teflon coated O ring seals Swinging head assembly storage to operational position opens orifice and activates water flow Unit remains in operation until head assembly is returned to storage position Furnished with in line strainer to protect valve from debris and foreign matter mounting 16 gauge stainless steel cabinet with flanged rim for recessed mounting in wal Hot Link to Guardian Cut Sheet pdf 1212013 STLOC FV Morrison SSTLC CPLE Monson Biosafety Shower Eye Testing BRDG Weekly as required by OSHA and ANSI standards SHOWER TEST Configure Shower funnel ring entirely over on top of shower head and hold handle while testing shower 2 Drop funnel shiold inside 5 gal bucket 3 Pull down shower handle and let run until bucket is 80 full 4 Verity flow rate and clear water stream EYE STATION TEST 1 Place 18 wide gt 8 gallon bucket below shower as shown on stool or other support Pull down eye handles approximately 70 degrees until eye spray handles begin to flow water adjust bucket as needed betore pulling 10 full down horizontal position Let flow run until
34. ndatory Hot Link to OSHA Standards for Chemical Safety in Labs htm STiCC CPLSMorison 12 11 2013 Page 18 Biosafety Regulations General Principles Hot Link to OSHA Standards for Chemical Safety in Labs htm A General Principles for Work with Laboratory Chemicals In addition to the more detailed recommendations listed below in sections B E Prudent Practices certain general principles including the following presses 1 Itis prudent to minimize all chemical exposures Because few laboratory chemicals are without hazards general precautions for handling all laboratory chemicals should be adopted rather than specific guidelines for particular chemicals 2 10 Skin contact with chemicals should be avoided as a cardinal rule 198 2 Avoid underestimation of risk Even for substances of no known significant hazard exposure should be minimized for work with substances which present special hazards special precautions should be taken 10 37 38 One should assume that any mixture will be more toxic than its most toxic component 30 103 and that all substances of unknown toxicity are toxic 3 34 3 Provide adequate ventilation The best way to prevent exposure to airborne substances is to prevent thelr escape into the working atmosphere by use of and other ventilation devices 32 198 4 Institute a chemical hygiene program A mandatory chemical hygiene program designed to minimize
35. or 10 seconds until screen moves from monitoring to Setup and Run options STLCC CPLSMorison 12 11 2013 Page 28 Biosafety Local Service Hoods July 2013 sab rene Fume hoods all passed vell within range of acceptance Nes due 72014 On a ns Rand Wood rech aisats com Cent Ds 5199 ed nasa 0702 5 SOTSHAAS 22 Biosafety Hoods BRDG Inspection Reports July 2012 STCO CPLSMorison 12112013 48 Wx 34 Dx 63 H Service Fittings Color Code Hot Water Cold Water Dark Green Gas Dark Blue Air Orange Vacuum Yellow Disiled Water White Siam Nitrogen Brown Oxygen Light Green Hydrogen Pink Special Gases Light Blue Hot Link to Kuwaunee Specifications and Test Criteria pdf Hot Link to Kuwaunee Service Fitting Requirements pdf 22 STLCC CPLSMorion 12 11 2013 BioSafety Cabinet Labconco Class Il Type 2 Purifier Logic Series BRDG 8 Purifier Digital De Series Class Il A2 Nominal Width 6 Exhaust Volume 403 443 Lighting Fluorescent Ultraviolot Built In Options UV Light 2 service fixtures 34091218831 RevA 3460809 BASE CAT 079460 03 Hot Lin
36. r For Oxygen MSA10074137 363 31 PPM or by volume readout Powerful audible alarm complemented by vibrating and visual alarms standard Backit display Go display modo User adjustable alarm and calibration gas setpoints Water resistant IPG6 67 certified by a third party Intorchangeable smart sensors monitor oxygen or any one of many toxic gases Docking Station compatible year datalogging capacity minimum Standard STEL TWA sLifetime warranty Minimum 2 600 hours continuous operation replaceable CR2 Lithium battery STiCC CPLSMorison 12 11 2013 Biosafety Regulations Material Safety Data Sheets MSDS At STLCC FV the official Biology and Biotechnology printed MSDS library binders are located on the hallway directly outside the office of the lab manager Ginny Naumann Hot Link to online resources for MSDS information from various public Industry and Government sources some sites require registration and fees after trial period html Hot Link to online resources for 350 000 MSDS at the Hazard Communication Library Free no registration required no cookies html Page 24 LS Morison 12 Clean Bio sate Gowning Design Features Bete 1 Secure lockers for personal property o 9 oo S 2 Shoo cleaner w brushes
37. rom the m 1 013 ot Wino 2 nob ne Rn Biosafety Labconco A2 Cabinet Final Purging Unload Wipe Down Upon completion of work the cabinet should be allowed to operate for two to three minutes undisturbed to purge airborne contaminants fiom the work area Unloading Materials and Equipment Objects in contact with contaminated material should be surface decontaminated before removal from the cabinet All open trays or containers should be covered before being removed from the cabinet Wipe Down down the interior surfaces of the cabinet with 70 ethanol or a suitable disinfectant and allow to dry Periodically lift the work surface and wipe down the area beneath it Inspect and clean the towel catch located at the rear of the work area beneath the work pan Dispose of rubber gloves appropriately and have lab coat laundered properly 4 Wash hands and arms thoroughly with germicidal soap Biosafety Biosafety Cabinet Labconco 2 Airflow OVERVIEW Select the right Purifier Logie cine foe your S application Feier eus Clics pe Mad ye niri ow a pues rn edm Ee im hc Sipe fer 1 A2 Aion eng pom ar wt gl Dae nde rari bow De wk gestion Tom Type A2
38. son 12 11 2013 Biosafety nx Ethidium Bromide Iter Invoice Dec 2013 Invoice Number P53980 Salesperson House Account sanden Invoie 080201 567342099 INVOICE for St Louis Community College To St Lovie Ce St Louis Community College Accounts Pa Center Plant Life BROG Park at DOPSC aoo South 1005 Warson Fon 122 St Lous 63102 2010 St Louis 63132 United States States Crd Shipment Carer Payment information Terms niet 30days An Richard Norris POS P1403 Orlered By T Bescrpton Val mom wei Sip Un Pace Entende SOM d ng Coram sr sro m STLCC CPLSMorison 12112013 lt lt Amount Paidin Biosafety First Aid Kit North Lab VWR BRDG Designed for use in classrooms and research or ciinical laboratories his kt contains of the components necessary to eat common injuries found in a laboratory environment kit is unitized for easy access of treatments and ease of inspection ANSI Z308 1 compliant North Safety 019750 00841 Kit Contents 6 Burn Iel Pain Relieving Gel 3 7 mL 1 8 oz Pouch 10 Antiseptic Wipes BZK 1 Water Jel Burn Dressing 10 2 x 10 2 4 x 47 6 Adhesive Bandage XL Plastic 16 Adhesive Bandage 2 5 7 6 1 x 3 Pla
39. stic 4 Gauze Pads 7 6 x 7 6 3 x 3 Sterile 1 Compress 10 2 4 Offset 1 Triangular Bandage Nonsterile 1 Eyewash with 4 Eye Pads and Strips 1 Instant Cold Pack 2 Adhesive Tape 1 3 x 2 3 m 1 2 71 2 1 Forceps and Scissors 2 Nitrile Gloves Medical Grade 1 Poison Control Directory SSTLCO CPLS Monson 12 11 2013 Biosafety Gloves Heat Resistant Autoclave GLOVES AUTOCLAVE 47CM 32885 826 Each of 2 42 24 Excellent hand protection for handling hot objects out of the autoclave or oven pliable and washable all cotton terry cloth gloves Heat resistant up to 282 C 45044 Gloves are bright orange for high Visibilty Length 47em 181 27 132010001 SSTLCO CPLS Monson 12 11 2013 22 Biosafety Desiccator Drybox Secador manual Hygrometer dial in the door allows for easy monitoring of relative humidity Case blocks 99 of UV light General description Dry storage of humidity senstilve products is now easier Secador cabinets assure moisture and dust free storage of valuable items like reagents and pipettes with their patented sealed design Available in small large models with either horizontal or vertical orientation these uniquely designed units are available totally clear clear with blue end caps and amber The stackable small model is perfect for maximizing storage space in a minimum footprint Cabinets are molded of a co polyester
40. terials required for the procedure Do not overload the cabinet Donor obstruct the front side or rear retum air grilles Large objects should nor be placed close together Slowly close the sash until it is in the correct operating position After loading the cabinet wait two to three minutes to purge airbome contaminants from the work area Work Techniques Biosafety Labconco 2 Cabinet Work Techniques Keep all materials at least 4 inches 100 mm inside from the sash amd perform all contaminated operations as far to the rear of the work area as possible Segregate all clean and contaminated materials in the work area Amange materials to minimize the movement of contaminated materials into clean areas Keep all discarded contaminated material to the rear of the work area Avoid moving materials or the operator s hands and arms through the front access opening during use Avoid the use of an open flame Use disposable lab ware or an electric incinerator as alternatives Use proper aseptic technique Avoid using techniques or procedures that disrupt the airflow patterns of the cabinet If there is a spill or splatter during use all objects in the cabinet should be surface decontaminated before removal Thoroughly disinfect the working area of the cabinet WHILE IT IS STILL IN OPER ATION to nrovent the releaze of contaminants f
41. tween walls and 5 tem 27 vent with lash arrestor Complete with grounding attachments three point latching system 5 1 27 raised leakproof sills and yellow enamel finish Equipped with a large warning sign FLAMMABLE KEEP FIRE AWAY Nush mounted locking handle and leveling legs Cabinets are supplied with one or two adjustable steel shelves Al cabinets comply with OSHA regulations and are designed in accordance withthe standards of Coda 30 3 Vent to outside required if used for Acid or Flammable storage Flammables Safety Storage Cabinets SECURALL Capacity W cm H inches Shelves Wi Securall VWR 1703 45 1092 165 1 x 45 7 43 65 18 Manual 2 153 337 A145 15001 668 STLCC CPLSMorison 12 11 2013 Pagese Biosafety Acid Storage Cabinet VWR Eagle BRDG Polyethylene Acid Corrosive Storage Cabinets Eagle Manufacturing Supper Eade 83 3 22 89x56 x 91 4 35x22x36 2 43 2 95 CRA P22 56618 604 Each 1 409 46 Nonmetallic cabinets are constructed of 100 polyethylene for excellent resistance to acid vapors and spills making them ideal for storing nitric sulfuric and hydrochloric acids Available in 15 11 bonchlop and 83 3L 22921 undercounter storage models hoy feature intemal and top sump capacities of at least 3 81 1841 storage for smaller containers in the doors lockable doors adjustable shelves and two 5 1 27 vents with caps Cabinets
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