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Lumen 200/Pro™ 13 - Prior Scientific, Inc.

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1. lifetime may vary depending on climatic conditions Cold and humid environments will extend lifetime hot and or dry environments will shorten it Though the outstanding UV performance will not markedly degrade during usage we recommend that the light guide is replaced in advance of the expected lifetime of expiration Final degradation is generally caused by the formation of bubbles in the liquid and optical output may then drop very rapidly Approximate lifetime 4 years Suggested replacement time 3 years Figures based on 23 C and 60 humidity General usage temperature range Min 5 C 41 F Max 30 C 86 F Should the temperature limits be exceeded the likely damage is the formation of bubbles inside the liquid These may be reabsorbed if the light guide is stored at room temperature for several days 10 Prior Scientific Unpacking The System Lumen 200 Pro Seclion 3 Id Identifying your system The Lumen200 Alarm reset Button PRIDR Lume n200 Display Power Switch Manual Shutter Control Microscope Adaptor Liquid Light Guide Liquid Light Guide connection 12 Prior Scientific The Lumen 200Pro Mains Power connection Filter Wheel and Shutter connections Liquid Light Guide Red Protective cap Quartz window Lumen 200 Pro 13 Adaptor for microscope and collimator 14 Prior Scientific Installation Lumen 200 Pro Section 4 15
2. Bulb Housing Hg Label on base of Connector Bottom of bulb white section Figure 4 1d Lumen Bulb 18 Prior Scientific 7 Lift the spring towards the bulb as you do so the bulb will be pushed into the correct position click the springs all the way into the Lamp Spring Restraints see Figure 4 1e Lamp Spring Restraint Lamp Spring Figure 4 1e Top section of the bulb clamp being pulled up with two fingers 8 The bulb is now firmly held vertically in place 9 Plug Data connector into base of bulb 10 Replace the bulb housing cover and replace the four hex screws Data Connector Figure 4 1f Data connections on Bulb Lumen 200 Pro 19 4 2 Connecting to the Microscope Once the bulb is installed see section 4 1 the Lumen is ready to be connected to the microscope l De 20 Place the Lumen in a safe location and ensure none of the fan outputs are obstructed Unpack the Liquid Light guide from the foil packaging and remove both plastic caps from the light guide Note It is important to remove the caps before connecting to the light guide to avoid damage on power up Unscrew the connector on the front of the Lumen and insert the Light guide a Ensure the light guide is fully inserted into the Lumen the light guide should be inserted by 58mm Tighten the connector unit resistance is felt the light guide is now firmly held in the Lumen connector Locate the coll
3. Contents Lumen 200Pro Lumen 200 Pro Manual Liquid Light Guide Adaptor for specific microscope Lumen 200 Bulb Power Cord Two Filter Wheel Cables Shutter cable Filter Wheel tool Hex key Lumen 200 Pro 7 General Information Section 2 8 Prior Scientific General Information 2 1 Lumen 200 Pro Specification Power Universal integral Power Supply Input 110 240V 50 60Hz Use within ambient temperature range 18 28 C Required clearance 100mm minimum 2 2 Lumen 200 The Lumen 200 version of the Lumen series is a stand alone standard unit and is furnished with a manual 6 position shutter 0 10 25 50 75 and 10096 The Lumen contains a 200 Watt Metal halide bulb which is temperature controlled The bulb is self aligning and is coupled via special optics to the Liquid Light guide which transfers the light to the Microscope For each major make of microscope an adaptor is available to connect the liquid light guide to the microscope 2 3 Lumen 200Pro The Lumen 200Pro version of the Lumen series contains a 6 position filter wheel and an advanced shutter 0 100946 in 1 increments The filter wheel and shutter require an external controller it is recommended that this 1s the Prior ProScan or PCI card the OptiScan II will control these accessories Lumen 200 Pro 9 2 4 Liquid Light Guide Information Liquid light guides have a limited lifetime independently of whether they are stored or in use However
4. a Sets the current filter wheel w acceleration to a Range is 4 to 100 Report the current filter wheel w maximum speed setting m TIT Sets the current filter wheel w maximum speed to m Range is 1 to 100 Lumen 200 Pro 31 Lumen 200Pro Standard Shutter Commands The Lumen 200Pro Shutter accepts the standard commands for a Filter Wheel Position 1 10 provide a standard range of light output from 0 100496 Command Arguments Response Description All end with cr 7 w f iff Fthe Iffis anumber move filter wheel w to filter position f current if f is a N move filter wheel w to next filter filter else R If f is a P move filter wheel w to previous filter If no wheel If f is a F report current filter on filter wheel w is fitted if f is a H performs a home routine E 17 will be If f is A wheel will auto home on controller startup returned If f is D wheel will NOT auto home on startup default FILTER w Text string Prints information about filter wheel w The information end is always a line saying END This allows for the addition of extra fields of information without effecting application software Users should always read lines in until the END is seen to keep in order to maintain compatibility Example FILTER_1 LLG_SHUTTER TYPE 3 PULSES PER REV 262500 FILTERS PER WHEEL 10 OFFSET 223500 HOME AT STARTUP FALSE END Reports the number n of filters on whe
5. 0Pro to a OptiScan or ProScan System The Lumen 200Pro requires two filter wheel connections to a ProScan II or OptiScan II system Connecting your system 1 Switch off the ProScan II or OptiScan II controller 2 Connect both Filter Wheel and Shutter ports on the Lumen to the Filter 1 and Filter 2 ports on the ProScan or OptiScan II system using the cables provided a Itis not important which Filter connections are used on the Controller but this should be noted for use with the command set described below b Tighten the screws to fix the cables in position 3 Switch on the Controller your Lumen 200Pro is now ready to accept commands a Itis not necessary to switch the Lumen 200Pro power on to control the Shutter and Filter Wheel these are powered by the attached controller Filter Wheel Light Attenuator Lumen 200 Pro 27 6 2 Connecting your Lumen 200Pro to Prior PCI controller The Lumen 200Pro requires two filter wheel connections to a Prior PCI system Connecting your system 1 Switch off the PCI controller computer 2 Connect the Attenuator shutter to FILTER 1 using the cables provided a Tighten the screws to fix the cables in position 3 Connect the Filter Wheel to FILTER 2 using the cables provided a Tighten the screws to fix the cables in position 4 Switch on the controller and computer your Lumen 200Pro is now ready to accept commands a Itis not necessary to switch the Lumen 200P
6. 4 1 Installing the Bulb Required equipment Lumen 200 Hex Key Prior LM200B1 Bulb Warning e Only use Prior LM200BI bulbs failure to do so may cause damage to the unit e Do not touch the inside of the reflector of the bulb e The bulb is delicate handle with care Instructions 1 Disconnect the Lumen from the mains supply f the lumen has been previously switched on wait for 30 mins to allow bulb to cool 2 Finda flat surface and place the lumen upside down on the surface 3 Unscrew the four Hex screws and remove the panel as indicated in Figure 4 1a below Hex Screws Figure 4 1a Bulb housing 4 Remove bulb from Carton See Figure 4 1b below a Open top of Carton b Remove V shaped Cardboard Holder 16 Prior Scientific c Push Cardboard Flaps back d Lift bulb out of carton Cardboard Holder Cardboard Flaps Figure 4 1b Bulb storage and transportation carton 5 Turn the bulb so that the cables and connector hang down into the bulb chamber Plug the brown connector on the bulb into power socket in bulb housing ensure it is pushed firmly into position a This will orientate the bulb with the Hg label facing upwards Lumen 200 Pro qu 6 Locate the bottom of the bulb as indicated in figure 4 1d below into the groove in the bottom lamp house clamp Figure 4 1c of the lamp housing Lamp Spring Restraint QUA o Lamp Spring Bottom Groove Figure 4 1c
7. Table of Contents Important Safety Information Section 1 i242 ei 1 1 Important Safety Information 1 2 Safety Precautions 1 3 Shipping Storage Precautions General Information DOES Dirette a Ea ati pa 2 Lumen 200 Pro Specification 2 0 Lumen 200 2 3 Lumen 200Pro 2 4 Liquid Light Guide Information Unpacking The System Section 3 Identifying your system Installation Section dolum 4 1 Installing the Bulb 4 2 Connecting to the Microscope Getting Started Section 5 5 1 Starting Up the Lumen 5 2 Shutting down the Lumen 5 3 When to Change the bulb 5 4 Changing Filters in the Filter Wheel Pro only 5 5 Routine Maintenance Advanced Operation Section G 6 1 Connecting your Lumen 200Pro to a OptiScan or ProScan System 6 2 Connecting your Lumen 200Pro to Prior PCI controller 6 3 RS232 Command Set 6 4 Error Codes Troubleshooting Section 7 7 1 Alarms and Warnings 7 2 Troubleshooting Replacement Parts Section 8 Returns and Repairs Section 9 Lumen 200 Pro Important Safety Information Section 2 Prior Scientific I 1 Important Safety Information e Use only as specified by the operating instructions or the intrinsic protection may be impaired e Keep this manual in a safe place as it contains important safety information and operating i
8. When to Change the bulb The Lumen Bulb is installed with a timer chip which counts the hours that specific bulb has been activated Once the bulb reaches the recommended lifetime of 2000hours an alarm sound on the lumen It is recommended that the bulb is changed at this point This alarm can be silenced using the button situated to the left of the display panel on the front of the lumen Once a bulb has reach 2000hours the alarm will sound on power up of the Lumen until the bulb is changed 5 4 Changing Filters in the Filter Wheel Pro only The Filter Wheel can hold up to 6 25mm filters these are held in position using the standard Prior mechanism Changing a Filter Equipment required Lumen 200Pro Filter Prior Filter block tool Hex key Instructions 1 Disconnect the Lumen from mains Power 2 Disconnect from controller 3 Ensure the unit has been allowed to cool for 30mins 4 Place the unit on a flat surface with empty space in front of the unit 5 Unscrew the hex screws as indicated in Figure 5 5a 6 Lift the top of the unit applying particular pressure to the front right corner to disconnect the internal connector See Figure 5 5a 7 Turn the filter wheel until the desired filter position is in the filter change position indicated in Figure 5 5b The filter number is displayed on the bottom right of the filter hole 8 Using the Prior Filter block tool unscrew the filter ring a Usethe two lugs in the Filter
9. are Users should always read lines in until the END is seen A typical response is shown below OPTISCAN INFORMATION DRIVE CHIPS 11111 JOYSTICK ACTIVE STAGE NONE FOCUS NONE FILTER_1 LLG SHUTTER FILTER_2 LLG_FILTER SHUTTERS 000 END Prior Scientific The filter wheel installed in the Lumen Pro accepts the following commands Command Arguments Response Description All end with cr 7 w f iff Fthe Iffisanumber move filter wheel w to filter position f current if f is a N move filter wheel w to next filter filter else R If f is a P move filter wheel w to previous filter If no wheel Iff is a F report current filter on filter wheel w is fitted if f is a H performs a home routine E 17 will be If f is A wheel will auto home on controller startup returned If f is D wheel will NOT auto home on startup default FILTER w Text string Prints information about filter wheel w The information end is always a line saying END This allows for the addition of extra fields of information without effecting application software Users should always read lines in until the END is seen to keep in order to maintain compatibility Example FILTER_1 LLG FILTER TYPE 23 PULSES PER REV 262500 FILTERS PER WHEEL 6 OFFSET 223500 HOME AT STARTUP FALSE END Reports the number n of filters on wheel w Sr fw fa Report the current filter wheel w acceleration setting SAF w
10. block tool to locate in the two holes of the filter ring Lumen 200 Pro 23 9 Remove the old filter replace the new filter taking care not to place finger prints on the filter 10 Using the Prior Filter block tool screw the filter ring to hold the filter a Do not over tighten the filter ring some filters will expand as they are heated by the light b Tighten until tight then unscrew by a small amount increase this for filters prone to heat expansion e g glass 11 Repeat for all required filters 12 Replace top of unit ensuring the internal connector is aligned 13 Replace hex screws 14 Reconnect controller a Test filter wheel 15 Reconnect Lumen mains power Figure 5 5a Fixing screws for Lumen 200 Pro Lift Cover Vertically supporting at front right Fixing Screws 24 Prior Scientific Filter Number Internal Connector Filter wheel Figure 5 5b Lumen 200 Pro with cover removed 5 5 Routine Maintenance The internal dust filters require cleaning every 12 months of use Remove the three screws holding the rear cover on Lift the rear cover off Remove filters Wash in warm soapy water Rinse filters in water and allow to dry Replace filters Replace rear cover ensuring the isolator switches are beneath the cover Replace screws Lumen 200 Pro 25 Advanced Operation Section 6 26 Prior Scientific Advanced Operation 6 1 Connecting your Lumen 20
11. el w Sr lm fa Report the current filter wheel w acceleration setting SAF w a Sets the current filter wheel w acceleration to a Range is 4 to 100 Report the current filter wheel w maximum speed setting m Prior Scientific mr Sets the current filter wheel w maximum speed to m Range is 1 to 100 Command Arguments Response Description All end with cr Lumen 200 Pro 33 The following are a list of commands specific to the Lumen 200Pro All end with lt cr gt LIGHT Reports a the output of light from the shutter in The command automatically locates LGG_SHUTTER Error 20 reported if no shutter detected LIGHT n Sets the output from the shutter to n where n can be set between 1 100 The command automatically locates LGG_SHUTTER If n is h the shutter will perform a home routine NO 00 AI QN tA A UYU N LIGHT Sets the filter wheel position P to n light output i e LIGHT 4 45 Sets position 4 to 45 light output Use 7 n 4 to move filter to position 4 where n is the Filter Wheel port which the shutter is attached STANDARD 10 Position Shutter settings are Position Light output 1 0 11 22 33 44 22 66 77 88 LIGHT NE ra Reports n the output of position p 34 Prior Scientific 6 4 Error Codes If a command is not valid a response of E n is returned the n specifying an error type as listed below Machine or human readable messages are chosen u
12. ials Lamps may be return to Prior Scientific providing they are return in their original packaging e Before replacing a fuse DISCONNECT THE EQUIPMENT FROM THE MAINS SUPPLY e Ensure that the mains switch IEC socket mains plug is easily accessible to allow the unit to be switched off Warnings Caution A A A Eye damage may result from directly viewing the light produced by the lamp used in this product Always make sure the light guide is properly inserted into the collimator which is firmly attached to the microscope using the adaptor provided and into the Lumen 200 Pro before turning on the power to the unit Never look into the emitting end of a light guide The light could severely damage the cornea and retina of the eye if the light is observed directly Appropriate eye shielding must be used at all times clothing should be used to protect exposed skin Never place the end of an emitting light guide near skin as this may result in burning and damage to the skin Never place the end of an emitting light guide near a flammable substrate as sufficient power is emitted from the light guide to ignite flammable substances Prior Scientific 1 2 Safety Precautions The Lumen 200 has protection features built in to avoid unintentional expose to UV radiation In addition to this protection please observe the following safety instructions Definitions of Labels Warning read instructions to determi
13. imating lens supplied to attach the light guide onto the microscope loosen the screw on the back of the collimating lens Firmly push the light guide into the hole ensuring it has reached it end stop and tighten the screw a The Lumen is supplied with a collimating lens specifically designed to fit your microscope the supplied collimating lens will only fit the specified make of microscope Prior Scientific Getting Started Lumen 200 Pro Section 5 21 5 1 Starting Up the Lumen Warning e Do not power up the lumen without the light guide attached to both the Lumen and Microscope e Only power up the Lumen when it is installed on a level surface 1 Ensure the light guide is attached to both the Lumen and Microscope 2 Connect the power cable to the Lumen 3 Switch the Lumen power switch on 4 Allow 1 5 minutes for light to reach 70 of output 5 Allow 30 minutes for the Lumen to reach operational temperature 6 Warning Do not power down the unit within 30 mins of power up This may reduce the effective lifetime of the bulb 5 2 Shutting down the Lumen The following warnings apply as damage to the bulb may result if instructions not followed Warning Do not shut the unit down within 30 minutes of powering up the unit Warning After shutting down the unit allow 30 minutes before re powering up or changing the bulb Failure to do so is likely to result in damage to the bulb 22 Prior Scientific 5 3
14. isplay on the front of the Lumen for error message Check to see if fan vents are covered or blocked Problem Either Filter Wheel or Light Attenuator will not operate Suggested Solution e Confirm that the D connectors on the side of the Lumen are connected securely into the filter wheel ports of a Prior ProScan PCI or OptiScan controller e Check your Controller for error messages 40 Prior Scientific Replacement Parts Lumen 200 Pro Section 8 41 Replacement Parts Description Prior Bulb Fuse Liquid Light Guide Filter Wheel Cable Filter Changing Tool Olympus Adaptor Zeiss Adaptor Nikon Adaptor Leica Adaptor 42 Part Number LM200BI W3377 LMLGM2M H300 H1165 LMIOOL MIOZS L LMIONI L MIOLC Prior Scientific Returns and Repairs Section 9 44 Prior Scientific Returns and Repairs Should you experience problems with your Lumen System and want to send it back for service warranty or otherwise a Return Material Authorisation RMA number must be obtained from the appropriate Prior Scientific office before returning any equipment For North and South America contact Prior Scientific Inc and for the rest of the world call Prior Scientific Instruments Limited on the telephone numbers shown below Prior Scientific Instruments Limited Unit 4 Wilbraham Road Fulbourn Cambridge England CB1 SET Telephone 01223 881711 FAX 01223 881710 email uksales prior co
15. m Lumen 200 Pro Prior Scientific Inc 80 Reservoir Park Drive Rockland MA 02370 1062 USA Telephone 781 878 8442 FAX 781 878 8736 email info prior com Part No W3402 Issue B 45
16. ne possible hazard Caution Read these operating instructions fully before use and pay particular attention to sections containing this symbol Use only as specified by the operating instructions or the intrinsic protection may be impaired Warning surface may be Hot A Warning UV Output Danger Electrical Shock hazard Lumen 200 Pro 5 Monitoring of the unit manual operation e The level of energy supplied is sufficient to ignite flammable substances A During operation the unit must be attended at all times by a qualified operator The unit must not be left unattended while left on If an operator leaves the work area of the unit the lamp power must be switched off 6 Prior Scientific 1 3 Shipping Storage Precautions e NEVER SHIP THE UNIT WITH THE BULB INSTALLED e Always use the original packaging for shipping and storing purposes Unpacking and Inspection e Carefully unpack the unit and retain packaging to return equipment for servicing e Ifthe equipment appears damaged in any way return it to sales outlet in it s original packaging No responsibility for damage arising from the use of non approved packaging will be accepted e Ensure all items and accessories specified are present If not contact your local sales outlet Lumen 200 Standard Contents Lumen 200 Lumen 200 Pro Manual Liquid Light Guide Adaptor for specific microscope Lumen 200 Bulb Power Cord Hex key Lumen 200Pro Standard
17. nstructions e Before using the system please follow and adhere to all warnings safety and operating instructions located either on the product or in this User s Manual e Do not expose the product to water or moisture e Do not expose the product to extreme hot or cold temperatures e Do not expose the product to open flames e Do not allow objects to fall on or liquids to spill on the product e Connect the AC power cord only to designated power sources as marked on the product e Make sure the electrical cord is located so that it will not be subject to damage AA AA e Always disconnect power from product before connecting the components together e Use only the power supply cord set provided with the system for this unit should this not be correct for your geographical area contact your supplier e Donotin any way attempt to tamper with the product doing so will void the warranty and may damage the system This product does not contain consumer serviceable components all repairs or services should be performed by Authorised Service Centres contact your local dealer for details A e Ensure that the ventilations slots in the controller case are free from blockages Accord with Disposal Laws e WARNING The method in which the lamps are disposed of must e WARNING Hg LAMP CONTAINS MERCURY Manage in A comply with the local rules amp regulations for disposal of Lumen 200 Pro 3 hazardous mater
18. orPCI manual for instructions on using HyperTerminal e Send HOME command to filter wheel L200PRO only to insure it is not obstructing the optical path Confirm that filter position in the light path does not contain a blanking plate Problem On initial start up illumination is not bright Suggested Solutions e The unit requires 5 mins to warm up and 15 30mins to reach full brightness e Check that Liquid Light Guide is installed correctly see section 4 for installation instructions e Check that bulb is installed correctly See section 4 for installation instructions Note you must wait 30 minutes after shutting off the illuminator before opening the access panel Problem Just changed bulb and light is significantly less than normal Suggested Solutions e Check that bulb is installed correctly See section 4 for installation instructions Note you must wait 30 minutes after shutting off the illuminator before opening the access panel Lumen 200 Pro 39 Problem Just changed bulb and alarm sounds continuously Suggested Solution e Check for error messages on display e Turn off L200 and confirm that the Data Connector is installed completely into the PCB located at the base of the bulb See section 4 1 Problem Alarm sounds every time Lumen is turned on Suggested Solution e Check display on front of Lumen for error message Check to see if the bulb has exceeded the recommended lifetime e Check the d
19. ro power on to control the Shutter and Filter Wheel these are powered by the attached controller Filter Wheel Light Attenuator 28 Prior Scientific 6 3 RS232 Command Set A description of how to connect to the ProScan or OptiScan systems is supplied in the OptiScan II and ProScan II manual The following is the RS232 commands applicable to the Lumen 200Pro General Commands for identifying the Lumen 200Pro Identify which Filter Wheel port the Shutter and Filter Wheel are connected to using the command described below Command Arguments Response All end with cr decimal number Lumen 200 Pro Description Reports status as a decimal number and gives motion status of the Filter Wheel or Shutter of the Lumen Attached to the controller After binary conversion convention is as follows F Fl A Z Y X D05 D04 D03 D02 DO1 D00 Parameters a where a is the axis or resource F Filter wheels FI Filter wheel 1 F2 Filter wheel 2 When the optional parameter is used the binary word is just for the axis requested F is for filters both Filter Wheel and Shutter and would return 0 to 3 depending on if they are in use 29 30 Text string Reports information about the peripherals currently connected to the controller The information end is always a line saying END This allows for the addition of extra fields of information without effecting application softw
20. sing the ERROR Command ERROR ERROR DESCRIPTION CODE 1 NO STAGE nosace ARGI OUT OF RANGE ARG6 OUT OF RANGE INCORRECT STATE WHEEL NOT FITTED Lumen 200 Pro 35 Troubleshooting Lumen 200 Pro Section 7 37 Troubleshooting 7 Alarms and Warnings The Lumen may display several warnings the following table explains the actions recommended on hearing an alarm Message Alarm reason Quiet Alarm Actions Bulb Fault 1 Software not recognising No Switch off unit hr count from bulb Check bulb for damage and check connections Bulb Fault 2 Bulb failure Yes Switch off unit Hold button for 5 10s Check bulb for Reset to clear alarm damage and check connections Over Temp Bulb over temperature Yes Check vents at rear Fault Hold button for 5 10s of unit are not Reset to clear alarm blocked or covered CHANGE Bulb reach 2000hr Yes Change bulb BULB lifetime limit Hold button for 5 10s 38 Prior Scientific 7 2 Troubleshooting Problem On initial start up no light output from Lumen200 Suggested Solutions e Confirm unit is plugged in and there are no error codes on front display e Confirm that the light output knob is not rotated to the 0 position L200 e Check shutter position L200PRO only position to confirm if it is in the fully closed position This can be done by sending the LIGHT command in HyperTerminal See ProScan OptiScan or Pri

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