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REACH-IN CO2 INCUBATOR OPERATIONS

1. REACH IN CO2 INCUBATOR OPERATIONS MANUAL For Models 7400 25 7400 33 7401 25 7401 33 7402 25 7402 33 7403 25 7403 33 Dear Valued Customer Thank you for purchasing CARON Products amp Services equipment We appreciate your business and look forward to being your preferred supplier of controlled environment equipment products in the future At CARON we are committed to continuous quality improvement Our goal is to supply our customers with highly reliable equipment at a fair price In order to openly monitor our performance we would appreciate your feedback on our products and services If you have questions or any suggestions for improvement based on the installation or operation of the equipment you have purchased please contact our service department at service caronproducts com or 740 373 6809 Thanks again for your business 7400 Series Operations Manual Rev B 02 24 15 Page 2 of 88 TABLE OF CONTENTS DECHION T Warranty 5 Section 2 Equipment nnn nnn 10 Section Equipment Installation eere res 12 Unpacking Choosing a Location Preliminary Cleaning Installing the Port Stopper Installing the Shelves Leveling the Unit Connecting the Drain Line Water Supply for Humidity Connecting CO2 supply Connecting Elec
2. Keypad is locked Passcode must be entered to unlock Ralurn To un lock the touchscreen Unlock From the previous Alert Keypad is Locked pop up screen press the Unlock button The Enter New Passcode window will pop up Enter Current Passcode 7400 Series Operations Manual Rev B 02 24 15 Page 71 of 88 Enter the digits 1 2 3 4 press 5 Enter when complete The Control Lock Icon will change back to the unlocked position Tu 19 35 AM d Control Lock Icon unlocked Changing the passcode To prevent un authorized and accidental changes being made to the chamber the touchscreen can be locked out The passcode is required to lock out the controls and the same passcode is used to un lock it The factory default passcode is 1234 This passcode can be changed by the user to create a unique 4 digit passcode The current passcode is required to change the passcode To lock the touchscreen Settings Control Lock Button Icon unlocked Settings xx Press the Settings button 7400 Series Operations Manual Rev B 02 24 15 Page 72 of 88 We 9 10 AM En Home Password A Password na Button Maint Setu Facto N d Password fa Press the Password button We 9 10 d Heme t Keypad is Unlocked ve Settings Passcode must be entered to keypad si yP L 2 1 lock Lockout To ch
3. When the Auto Export button is selected this will retrieve the data from the previous time when data was retrieved up to the current time when flash drive was removed from USB port USB flash drive icon Info button We 8 10 A 5 Automatically export stored data logaing to Hash drive when first inserted into USB port Export all history data to flashdiive Otherwise will only export history data from last export date Eject the flashdrive from the USB port Eject button When flash drive is inserted into the USB port a USB flash drive icon and flashing Info button appears in the status bar indicating that the data is being downloaded to the flash Eject drive Once Info icon stops flashing select the Eject button Wait until the USB icon disappears to safely remove the flash drive from the USB port Note Press the Eject button before removing the flash drive from the chamber otherwise there could be the risk of corrupt data Here is a graphic to illustrate how the data retrieval works Flash drive Flash drive Flash drive Removed Inserted Removed History back to when chamber was turned on Time Continuous data log 5 min interval NA Auto Export Not all Data Auto Export All Data 7400 Series Operations Manual Rev B 02 24 15 Page 40 of 88 Extended Temperature Range EXTD302 The extended temperature range is a factory installed option for model 74
4. keypad For a set point of 20 press B t then D followed by the Enter key Correct any mistakes with the Clear button and reenter the value Once the Enter key has been pressed the pop up keypad disappears and the screen returns to the Setpoint display with the new value of 20 0 C in the temperature set point button Temperature m ROME Setpoint Button Press the 4 Home button to return to the main screen 7400 Series Operations Manual Rev B 02 24 15 Page 26 of 88 Changing the Humidity Set point HUMD304 HUMD307 option Only controlled humidity will be displayed on the touchscreen Evaporative pan humidification will not be displayed The steps below walk through an example of changing the humidity set point Here is the display of the home screen Setpoint Button Actual Humidity E setpoint m To set the humidity set point press the the screen Setpoint button on the right side of Humidity Setpoint Button 7400 Series Operations Manual Hev B 02 24 15 Page 27 of 88 Enter the new humidity set point on the keypad as desired and press X when complete Press the 7400 Series Operations Manual We 9 10 4 8 Home button to return to the main screen Rev B 02 24 15 Home Button Page 28 of 88 Changing the CO Set point If an alternative set point is required the following steps can be taken Setpoint Button Tu 10 35 AM
5. 4 i 2221802 mE 2107 Actual setpoint go To set the CO set point press the screen Setpoint button on the right side of the We 9 10 4 CO Setpoint Button 5 0 Once the setpoint screen appears press the CO Setpoint button 7400 Series Operations Manual Rev B 02 24 15 Page 29 of 88 Enter New CO2 Setpoint Keypad Enter the new CO set point on the keypad as desired and press Enter when complete Home Button m Press the Home button to return to the main screen 7400 Series Operations Manual Rev B 02 24 15 Page 30 of 88 SECTION 6 OPTIONAL ACCESSORY OPERATION Using the Carboy Water System BOTL301 To fill the carboy while attached to the incubator unscrew the cap Fill carboy with distilled or deionized water see Connecting the Water Supply section for details The carboy holds 2 5 liters If the carboy must be removed in order to fill it up first disconnect the tubing between the carboy and incubator by pressing the metal lever at the tubing connects disconnects at the bottom of the carboy Then unscrew the four mounting screws and remove the carboy After re attaching the carboy connect the tubing by simply pressing the plastic fittings into each other Screw Cap Mounting Screws 4 total Tubing Connects Disconnect to Tubing To Carboy Incubator 7400 Series Operations Manual Rev B 02 24 15 Page 31
6. Problem Unit temperature is above below temperature set point e Has the unit s temperature set point been recently lowered raised and if so has the unit been allowed 12 hours stabilize at the new set point e Has the inner door been recently opened for an extended period of time e 5 the access port stopper in the right side of the cabinet installed e s the condenser filter on the front of the cabinet clean Unit humidity level is above below humidity set point 5 the unit connected to a water source as specified in the installation section of the manual e Has the unit been leveled to insure the cabinet drain works correctly e The cabinets drain line uses gravity to remove water Does the drain line have any rises in it above the cabinet s drain level that could be trapping water e Has the units humidity set point been recently lowered raised and if so has the unit been allowed time to stabilize at the new set point Has the inner door been recently opened for an extended period of time e 5 the access port stopper in the right side of the cabinet installed e s the condenser filter on the front of the cabinet clean Unit CO2 level is above below the CO2 set point e s the unit connected to a pressure regulated CO2 source as specified in the installation section of the manual e Has the units CO2 set point been recently lowered raised and if so has the unit been allowed time to stabilize at the new set point e
7. service To remove the pen cap gently lift the pen arm away from the chart paper Remove the black plastic pen cap to expose the fiber tip of the ink pen and gently place the pen back onto the chart paper Do not let the pen arm snap back onto the chart paper his will flatten the fiber tip of the pen and will no longer give you a fine line marking on the chart paper Place the pen cap in a safe place for future use If the stylus does not touch the chart adjustment can be made by slightly bending the metal pen arm in the center towards the chart paper Do not use more pressure than is necessary to create a fine line marking on the chart paper As the pen ink supply runs out the pen color will become lighter This indicates that the pen should be replaced Replacement of the Pen Hecorders that are equipped with fiber tipped cartridge pens will have a cartridge that is color coded red to designate pen number one and an optional cartridge that is color coded blue to designate pen number two The pen cartridge is securely fastened to the metal pen arm using a special U clip tab For ease of replacement it is suggested that the two screws that hold the pen arm be loosened and the pen cartridge and metal pen arm be removed as an assembly Unsnap the plastic U clip tab of the 7400 Series Operations Manual Rev B 02 24 15 Page 46 of 88 pen cartridge from the metal pen arm remove and discard the old pen cartridge Replace the new cartridge
8. 7400 25 3 7400 33 3 7401 25 3 3 7401 33 3 7402 25 3 7402 33 3 7403 25 3 7403 33 3 Classification Electrical equipment intended for residential commercial and lighting industrial environments Rated Voltage 220 240 ac Rated Frequency 50Hz Meets the essential requirements of the following European Union Directive s using the relevant section s of the normalized standards and related documents shown EMC EN 61326 CISPR 11 2004 Class B Laboratory Equipment Immunity Measurement amp Control requirements Performed according to EMC Directive 2004 108 EC IEC CISPR 11 1997 A1 1999 A2 2002 EN 55011 1998 A1 1999 A2 2002 FCC CFR47 Part 18 Safety EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use part 1 General Requirements M Tp By Dave Figel Engineering Production Manager CARON Products amp Services Inc 7400 Series Operations Manual Hev B 02 24 15 Page 88 of 88
9. services We also offer preventative maintenance contracts on our equipment Contact our service department for details at 740 373 6809 or visit us on the web at WWW caronproducts com Recommended Daily Maintenance Checks e Check the Temperature humidity and CO2 displays versus set points e Check for and correct any alarm condition Recommended Monthly Maintenance Checks e Check to ensure the drain in the bottom of the unit is draining properly e Check front air intake filter If it is dirty replace it with CARON part number FLTR301 Washing the filter will result in poor performance Recommended Annual Maintenance Checks e Disinfect all interior surfaces with a general purpose laboratory cleaning agent e Perform a complete calibration of the temperature humidity and CO2 systems e A full validation is recommended for GMP facilities each time a unit is installed moved or undergoes significant repair Contact CARON s service department to schedule on site validation 7400 Series Operations Manual Rev B 02 24 15 Page 79 of 88 page intentionally left blank 7400 Series Operations Manual Rev B 02 24 15 Page 80 of 88 SECTION 13 SPECIFICATIONS Temperature 10 Above Ambient to _ 60 20 C to 60 gROD menm Control Temperature Ten Sensor ange Humidity 3 RH nm Control ensor CO2 Range 0 20 CO2 CO2 Control 0 1 CO2 Infrared 32 W x 27 32 W x 27 D i 32 W
10. Connected UV lamp will come out with it specific warnings and instructions 7400 Series Operations Manual Rev B 02 24 15 Page 43 of 88 9 Discard used UV lamp A Follow local regulations for disposing lamps 6 Insert new UV lamp into lamp connector socket 7 Install UV light housing cap with attached new UV lamp into UV light housing 8 Re attach ground clip Ground clip must be securely attached to UV light housing to reduce risk of electrical shock 9 Install left access panel 10 Plug power cord in and turn chamber on 7400 Series Operations Manual Rev B 02 24 15 Page 44 of 88 Interior Electrical Outlet OUTL305 OUTL320 An optional interior electrical outlet is available to supply power to small interior appliances such as shakers or stirrers It is not intended to power high current draw devices Each outlet will handle up to 1 0 Amp Incubators with a single interior outlet will handle 1 0 amps total Incubators with three outlets will provide 3 0 amps total All outlets are resettable GFI protected Other outlet configurations can be purchased OUTL305 OUTL310 US outlet is 115V 60Hz Standard on model 7401 7402 amp 7403 60Hz incubators OUTL306 OUTL311 European Schuko outlet Standard on model 7401 7402 amp 7403 50Hz incubators OUTL307 OUTL312 OUTL318 UK British outlet OUTL308 OUTL313 OUTL319 Australia outlet OUTL309 OUTL314 OUTL 320 Brazil outlet 7400 Series Operations Manual
11. De viv Each item can be turned on to check the condition of that device or parameter to aide in diagnosing a problem Output 2 Press the Output 2 button for other parameter buttons 7400 Series Operations Manual Rev B 02 24 15 Page 77 of 88 We 8 10 AM a Home control to set point while this screen displayed this Warning z Unit will not control to set poin 2 7 7 1 Sellings Dryer Tout a Dec viv Factory 17 CO viv C out Output 2 Def viv Lout Pu Chamber control amp functionality is restored as soon as the screen is exited Home Settings or Factory buttons When finished with diagnosis in Output 1 or Output 2 screen press the Factory button to return to that screen Once you go back to the Factory screen all parameters that were selected in Output 1 or Output 2 screens will reset to off position 17 Press the Home button to return to the main screen 7400 Series Operations Manual Rev B 02 24 15 Page 78 of 88 SECTION 12 PREVENTATIVE MAINTENANCE Your CARON incubator has been robustly designed to minimize performance problems However regular maintenance is very important for continuous trouble free operation As a general rule CARON recommends an annual calibration check of the temperature humidity CO2 systems CARON offers a full range of on site calibration and validation
12. Password a n Butto Factory Press the Factory button 7400 Series Operations Manual RevB 02 24 15 Page 75 of 88 b Factory Configuration From the factory menu four items can be selected Press the Configuration button to view the chamber s contiguration Model Software Number revisions Board 1 0 HMI 1 0 System Functions ____ amp O pt ONS e Configuration Output Percent From the Factory screen press the Output Percent button to view the current percent output level of each control parameter Tu 10 35 AM 4 d 0 0 0 0 Faclory Output gt Perceni 10 0 7400 Series Operations Manual Rev B 02 24 15 Page 76 of 88 We 9 10 AM 4 d Output Cu z 1 amp 2 Modal A Output 4 Pane Xi Buttons lt Details ES Percent 11522 amp Faclory IE Output 1 Output 2 in the factory page will temporarily halt chamber Navigating to the Output 1 or Output 2 screens A control amp functionality To individually and manual control each output variable from the factory screen press the Output 1 button Note Based on the chamber model number and options not all functions will be present We 3 10 AM Home i Warming gt Unit wi control to get pointy li ili thi creer i Blower s Outlet 2 PE Air Ht Buzz Factory Aux Ht zu C viv Output 1 Door Ht H viv Refrig
13. are not covered under warranty WARRANTY DEFINED Caron Products amp Services Inc herein after CARON hereby warrants that equipment manufactured by CARON is free from defects in materials and workmanship when the equipment is used under normal operating conditions in accordance with the instructions provided by CARON COVERED e Parts and labor for a period of two 2 years from date of shipment e Any part found defective will be either repaired or replaced at CARON s discretion free of charge by CARON in Marietta OH Parts that are replaced will become the property of CARON e f CARON factory service personnel determine that the customer s unit requires further service dependent of the model involved CARON may at its sole discretion provide a service technician to correct the problem or require the return of the equipment to the factory or authorized service depot e CARON will have the right to inspect the equipment and determine the repairs or replacement parts necessary The customer will be notified within a reasonable time after inspection of any costs incurred that are not covered by this warranty prior to initiation of any such repairs NOT COVERED e Calibration of control parameters Improper installation including electrical service gas and water supply tubing gas supplies room ventilation unit leveling facility structural inadequacies or ambient conditions that are out of specification Cost of expr
14. back of the unit Repeat the process for Tank 2 Turn on the regulated gas supplies and check the connections closely for leaks To access the internal Gas Guard 7400 Series Operations Manual Rev B 02 24 15 Page 41 of 88 Settings Setpoint Button 009 ps E Settings Xxx Press the En LI Rej i Gas Guard Button Gas es Guard SS Press the Gas Guard button PE Active Tank mU Tank 1 Tank 2 1 Press the B Tank 1 Tank 2 button will manually switch the tanks The factory default master tank is Tank 1 When the appropriate gas pressure is supplied to both tanks the master tank will always be used as the gas source The unit will swap from the master tank to the alternative tank whenever a low gas pressure condition is detected 7400 Series Operations Manual Rev B 02 24 15 Page 42 of 88 Ultraviolet Germicidal Lamp LGHT602 Before removing access panel s disconnect electrical power Avoid exposure to direct or reflected germicidal ultraviolet rays Germicidal ultraviolet rays are A Replacing UV Light optional accessory 1 Turn off chamber and unplug power cord 2 Remove the incubator top access panel The housing is located in the back of the incubator 3 Unclip green wire with ground clip from UV light housing harmful to the eyes and skin UV Light Housing Cap Green Wire with Ground Clip 4 Pull UV light housing cap from UV light housing
15. may need done to ensure good running performance of the incubator The intent is to notify the user that the incubator needs attention thus minimizing the risk of a failure or alarm condition Some of the alert features are replace the atomizers humidified units only replace the air filter and equipment calibration is due Notification occurs via an Alert icon on the status bar When the Alert icon is pressed a pop up window will display the alert condition s Each alert condition parameter is factory pre set no adjustment is necessary Ihe Alerts are cleared through the Maintenance screen SMU 6 Alert icon Press the Replace CO2 Filter Close Window The Alert pop up window will appear displaying the alert message Close Window Press Close Window button to make the pop up window disappear 7400 Series Operations Manual Rev B 02 24 15 Page 62 of 88 Resetting on the Maintenance Screen Maintenance Menu Screen lets users check to see how much time is remaining on an item that may need routine service or calibration This is very convenient to inform the user that a particular item will need to have service performed soon After service has been completed the item needs reset and the alert will disappear We 9 10 37 07 Settings ed Press the Settings button We 9 10 Once the settings screen appears press the Maintenance button 7400 Se
16. of 88 Decontamination Cycle DECN301 Series 7400 7401 7402 and 7403 incubators can be equipped with an optional moist heat decontamination cycle The purpose of the cycle is to eliminate common microbial contamination in your incubator and extend the time between manual cleaning cycles The decontamination cycle is intended to be used as a reactive system to eliminate contamination It is not necessary to run the cycle at a fixed time interval The DECN301 option requires a humidity water source The water can be provided to the incubator using the following methods e For units with optional humidity control HUMD304 8 HUMD307 be sure there is an adequate water supply going to the incubator e For units with a water pan Model 7400 HUMD305 8 HUMD306 Fill the water pan with 2 liters of water and place in the chamber e Forall other units plug incubator floor drain and fill with 2 liters of water Before initiating a decontamination cycle the following steps must be completed 1 Power down the incubator 2 Remove all samples products equipment etc from the incubator 3 If the unit is equipped with an inner door kit or internal outlets remove these items before starting the decontamination cycle 4 Locate the removable sensor access plate in the rear plenum of the incubator Remove the plate by sliding the fasteners down 5 With power off remove the infrared CO2 sensor by unscrewing the connector on the rear of the
17. properly Kinks or elevations in the drain line above the cabinet drain will not allow the chamber to drain If a local floor drain is not available a variety of accessories are available through CARON customer service These accessories can also be viewed at WWW caronproducts com Models 7401 25 33 7402 25 33 and 7403 25 33 are non humidified chambers and have a 1 2 plug installed into the drain connection The drain will be required when a humidity option is purchased with the chamber 7400 Series Operations Manual Rev B 02 24 15 Page 14 of 88 Fill water pan also HUMD305 HUMD306 Use only distilled or deionized water with a resistivity between 50KQ CM and 1MQ CM and a pH of greater than 6 5 Using water outside this range will void your warranty Do not use water that contains chloramines Chloramines can damage internal rubber gaskets resulting in leaks A water pan is used to provide elevated humidity levels inside the incubator This feature is standard on 7400 models and optional for the others Using the handles provided remove the empty water pan from the bottom of the incubator Fill with 5 to 6 liters of water Re insert water pan back into the incubator Connecting a CO supply High concentrations of carbon dioxide can cause asphyxiation The use of CO monitors and alarms is recommended for areas where CO can collect The gas supply should be 99 pure and should not contain a siphon tube G
18. to inspect the equipment and determine the repairs or replacement parts necessary The customer will be notified within a reasonable time after inspection of any costs incurred that are not covered by this warranty prior to initiation of any such repairs NOT COVERED e Calibration of control parameters Improper installation including electrical service gas and water supply tubing gas supplies room ventilation unit leveling facility structural inadequacies or ambient conditions that are out of specification e Cost of express shipment of equipment or parts e Any customer modifications of this equipment or any repairs undertaken without the prior written consent of CARON will render this limited warranty void e CARON and their representative are not responsible for consequential incidental or special damages whether shipping damage or damages that may occur during transfer to the customer s point of use When the equipment is signed for at the customer s site ownership is transferred to the customer Any damage claims against the shipping company become the responsibility of the customer Repairs necessary because of the equipment being used under other than normal operating conditions or for other than its intended use Repair due to the customer s failure to follow normal maintenance instructions Parts considered consumable including light bulbs filters gases etc Damage from use of improper water quality Damag
19. 00 This extends the temperature range down to 10 Simply enter a lower temperature value when entering the temperature set point Built In Gas Guard System GASG302 An optional built in gas guard system is available to allow two tanks of CO2 to be connected to an incubator requiring approximately 15 psig of gas pressure The unit is designed to automatically switch from the primary tank to the secondary tank when low gas pressure of approximately 10 psig is detected on the primary tank This allows for a continuous supply of CO2 to an incubator after the primary tank is empty In addition the user is notified of a tank empty scenario via an audible and visual alarm The gas supply should be 99 5 pure and should not contain a siphon tube Gas pressure to the unit must be regulated to less than 30 psig Failure to do so could cause tubing to burst The CO gas supplies must be equipped with two stage regulators to ensure that the incoming gas to the unit is regulated to appropriate levels Ihe high pressure stage should have a 0 2000 psig range and the low pressure gauge should adjust from 0 30 psig When connecting the gas supplies adjust each tank output to 20 25 psig If the appropriate regulators are not available contact CARON customer service to purchase them Once the cylinder regulators are installed and adjusted on each tank connect the outlet of the regulator on Tank 1 to the hose barb fitting labeled Tank 1 on the
20. 9 volt DC battery back up in place will allow the recorder to continue to function normally for approximately 24 hours in the event of a power failure 7400 Series Operations Manual Rev B 02 24 15 Page 48 of 88 Built In Temp or Temp Rh 10 Thermal Recorders RCDR318 RCDR319 Built in 10 thermal pen recorders can be purchased with CARON incubators The recorders are shipped installed on the outer door of the incubator from the factory and require no further installation Unlike ink pen recorders the thermal recorders draw their own chart and control lines The 10 recorders have been setup at the factory in the following configuration 7 Day 24 Hour Temperature 0 100 C Humidity 0 100 for dual input recorders If this is not the ideal configuration for an application the recorder may be reconfigured using the following process Configuring the recorder In order to configure the recorder you will need to enter the set up mode of the recorder To enter the set up mode of the recorder press and hold the Change Chart button 3 until the thermal pen arm begins to move off scale and then release the button Note The green LED light will flash fast while the thermal pen arm is moving off scale Wait until the thermal pen arm has moved completely off scale and stops the green LED light will stop flashing and will be steady On Unscrew counter clockwise the chart hub knob at the center of the chart and remove the recording c
21. CED FEATURES Setting the time amp day The incubator has an internal real time clock that keeps track of the day and time It is set at the factory to Eastern Standard Time and may need adjusted for your time zone To keep the clock accurate it will need to be adjusted manually for daylight savings time changes lo set the day amp time Settings Button Settings F Press the Settings button Setup Button Press the Setup button 7400 Series Operations Manual Rev B 02 24 15 Page 66 of 88 Day Time Button 5 Day Time Press the Day Time button We 9 10 4 7 e Hour Button AM PM Button SulMo Tu We Tk Frisa VVE Minute Button Day of the Week Button 3 Press the Hour button Enter New Time In Hours Follow same procedure for setting up minutes 7400 Series Operations Manual Rev B 02 24 15 Page 67 of 88 AM To setup Press AM PM button and the words for AM and PM will toggle back and forth Su Mo Tu We Th EuSa VVE To set the Day of the Week press the Day of the Week button this button will scroll through the days of the week press until the abbreviated letters correspond to the actual day of the week Press the Home button to return to the main screen 7400 Series Operations Manual Rev B 02 24 15 Page 68 of 88 Locking the controls To prevent un authorized and accidental changes being made to
22. CO2 sensor The sensor unscrews where the orange cable meets the gray sensor housing Failure to remove the sensor will result in damage to the sensor and will not be covered by warranty 7400 Series Operations Manual Hev B 02 24 15 Page 32 of 88 6 Insert the orange cable into the grommet where the sensor was removed so that it is easily accessed when the sensor is replaced following the decon cycle 7 Replace the CO2 sensor plate and turn the incubator power switch back on 8 The infrared CO2 sensor can be disinfected using isopropanol Spray the cleaner on a soft clean cloth and wipe the sensor cleaner as damage to the sensor may occur Do not immerse the sensor in any type of Starting the Decon Cycle process from the home screen Settings button Settings Press the Settings button k Home 6 F Settings sa 7 Password Decon Cycle 4 d Selup n 3 Faclory i m Decon Cycle d Press the Decon Cycle button on the left side of the screen 7400 Series Operations Manual Rev B 02 24 15 Page 33 of 88 Home A ned Settings xx Decon fit Cycle Be sure and read the information that is on the screen before starting the Decon Cycle Not removing the sensor can cause damage to the CO2 sensor The decon cycle will heat the incubator interior surfaces to approximately 90C Do not open the exterior door during the cycle as contact with interior surf
23. Glass Door Port Right Side Left Side Access Port Access Port RTD Temperature Sensor Adjustable Sliding Shelf Infrared CO2 7400 25 33 Sensor Humidity Sensor Controlled Humidity only Outer Door Drip Trough Model 7400 25 shown The following incubator models have pre configured internal shelving and brackets tailored for particular applications used in cell culturing 7401 25 33 Multi Tier Shaker Ready CO2 Incubators 7402 25 33 Cell Roller Ready CO2 Incubators 7403 25 33 Shaker Cell Roller Ready CO2 Incubators 7400 Series Operations Manual Rev B 02 24 15 Page 10 of 88 SECTION 2 EQUIPMENT OVERVIEW CONTINUED Day Time Actual otatus Bar Actual Temperature Humidity Tu 10 35 AM 4 7 Set point Speaker Setpoint go Menu e Y lt 7 Settings Lockout oonga G Menu icon Parameter Display y Zones D 4 Home Screen Current screen We 9 10 AM 5 indicator Home A q N Settings A gt Navigation Alarms Calibrate Password Pane indicates menu depth Settings Screen Submenu categories 7400 Series Operations Manual RevB 02 24 15 Page 11 of 88 SECTION 3 INSTALLATION Unpacking Your new unit has been thoroughly packaged to avoid shipping damage However the unit should be fully inspected upon arrival before signing for receipt If the package has visual damage notes should be made on the freight bill and signed by the deliv
24. Has the inner door been recently opened for an extended period of time e 5 the access port stopper in the right and left side of the cabinet installed 7400 Series Operations Manual Rev B 02 24 15 Page 85 of 88 SECTION 16 SPARE REPLACEMENT PARTS General Temperature Related Part Number _ _ Description 0 Refrigeration Cooling Solenoid Humidity Related Humidification Solenoid Drain Tubing 3 8 7400 Series Operations Manual Rev B 02 24 15 Page 86 of 88 SECTION 16 SPARE REPLACEMENT PARTS CONTINUED CO2 Related Part Number CO2 101 Carbon Dioxide Sensor SOL 135 CO Injection Solenoid FIL 213 In line CO HEPA Filter FIL 115 HEPA filter Fuse Related ID f Description 115V 230V FUS3 Internal outlet fuse double duplex FUS 163 4A FUS5 Internal outlet transformer FUS 164 3A Fuse size varies depending upon whether the chamber has a single internal duplex outlet or two internal duplex outlets SECTION 16 SPARE REPLACEMENT PARTS CONTINUED Options Related Part Description Number 1 4 barb 1 4 push in adapter 1 4 1 0 vinyl tubing 1 2 D silicone tubing 7400 Series Operations Manual Rev B 02 24 15 Page 87 of 88 AREN DECLARATION OF CONFORMITY Caron Products and Services Inc 27640 State Route 7 Marietta OH 45750 USA C 2014 Declares that the product Designation 7400 Series Model Numbers
25. Rev B 02 24 15 Page 45 of 88 Operation of Temp or Temp Rh 6 Recorders RCDR316 RCDR317 Built in 6 ink pen temperature and or humidity recorders can be purchased with CARON incubators The recorders are shipped installed on the outer door of the incubator from the factory and require no installation Changing the chart paper Press and hold the change chart button on the recorder 3 for approximately one second until the pen begins to move to the left of the chart and then release the button Wait until the pen has completely moved off of the chart To remove the chart paper unscrew counter clockwise the chart hub knob at the center of the chart Remove the old chart paper and position the new one so that the correct line coincides with the time line groove on the chart plate He attach the chart hub knob and fasten securely against the chart Press and hold the change chart button 3 again for approximately one second until the pen begins to move back onto the chart and then release the button Check to make sure that the pen is marking on the chart paper If it is not then carefully adjust the pen arm to establish contact with the paper Chart recorder marking system This type of pen consists of a self contained ink reservoir with a porous plastic stylus which is snapped around the outer edge of the metal pen arm A pen cap is provided to extend the life of the ink pen during shipping or when the recording unit is not in
26. aces may result in burns If the outer door is opening during the decon cycle an audible warning alarm will occur Start Decon Cycle Press the Start Decon Cycle button to start the Decon Cycle The cycle will run for about 14 hours Do not open the door until the cycle is complete Powering the unit off during the Decon Cycle will abort the cycle During the Decon Cycle a status icon will appear in the Status Bar At this time the screen is locked and the Setpoint and Settings buttons cannot be used Decon Cycle Info To check the status of the Decon Cycle press the Decion Info icon in the Status Bar 7400 Series Operations Manual RevB 02 24 15 Page 34 of 88 Decon Cycle Info The Decon Cycle Info Screen will appear displaying the current stage that the Decon Cycle is in When the Decon Cycle is complete there is a message displayed on the Decon Cycle Info screen that tells you that the cycle is complete and that is safe to replace the CO2 sensor Decon Cycle Info m Close Window Press the Close Window button to return to the main screen 7400 Series Operations Manual Rev B 02 24 15 Page 35 of 88 Operation of the Data Logger DLOG301 The DLOG301option provides the customer with a means of logging data electronically for viewing at a later date Logged variables are temperature humidity CO2 and light intensity but only if the c
27. ach time the chart paper or fiber tip pen cartridge is changed you should make sure that each pen stops at the outer most temperature graduation of the chart paper Otherwise this pen offset will cause the unit to record an incorrect temperature on the chart Recorder Calibration 7400 Series Operations Manual Rev B 02 24 15 Page 47 of 88 If a calibration adjustment is required for a single pen recorder use the left 41 and right 2 arrow push buttons on the recorder to calibrate or move the pen s position on the chart to correspond to the temperature of the solution The arrow buttons must be held for approximately five seconds before the pen will begin to move For two pen recorders you must first select the pen that you wish to calibrate This is done by pressing the left 1 arrow button to select the red pen or the right 2 arrow button to select the blue pen The arrow button must be held down until the green LED light goes out After the green LED light goes out follow the instructions in step 3 above Battery Backup The green LED light remains a constant green color indicating that both the battery and the main power to the unit are good Refer to Figure 5 for the location of the green LED indicating light If the AC power were to fail or the battery becomes weak then the green LED light will begin flashing indicating that either you have lost the main power to the unit or it is time to replace the battery Having a
28. ange passcode enter j current passcode followed Passcode by new passcode Passcode gagag Press the Change Passcode button The Enter Current Passcode Keypad screen will appear 7400 Series Operations Manual Rev B 02 24 15 Page 73 of 88 Enter Current Passcode 1234 m s J The Enter New Passcode Keypad screen will appear Enter any new four digit passcode example 2 5 80 Then press gt Enter when complete We 3 10 E 4 4 Heme fh Keypad is unlocked settings s ck rai Passcode must be entered to get ock 7 Lockout Passcode changed to 2580 Change OOO Pas ode mmm The Lockout screen will tell you that the Passcode has been changed to a new value This is only time that the Passcode will be displayed on the Lockout screen Factory menu amp troubleshooting 7400 Series Operations Manual Rev B 02 24 15 Page 74 of 88 The chamber control system is equipped with advanced diagnostics features which allow the user to manually turn amp each electronically controlled system The factory menu can be used to e View the current chamber configuration e See the percent output of the control system e Manually and individually toggle any output To access the Factory Menu Settings Button Settings Press the Settings button We 9 10 AM Factory
29. as pressure to the unit must be regulated to 15 20PSI Failure to do so could cause tubing to burst The CO supply should be 99 and not have siphon tubes CO pressure should be regulated to 15 20 psi CO tank regulators REGL101 can be purchased through CARON customer service Once the cylinder regulator is installed connect the outlet of the regulator to the hose barb fitting using the tubing and clamps provided An inline HEPA filter is provided to remove any contaminants in the CO2 gas supply Check the connections closely for leaks 7400 Series Operations Manual Rev B 02 24 15 Page 15 of 88 If the unit is equipped with a built in gas guard system GASG302 there will be 2 gas inlets Each of the inlets should be connected to an individual gas tank as described above Connecting Electrical Power Connect each incubator to a grounded circuit Failure to do so could result in electrical shock The unit requires a dedicated electrical outlet See table below for model specific power required and connection Model Power Requirements Plug Connection 115V 60Hz 16A FLA NEMA 5 20 230V 60Hz 10A FLA NEMA 6 15 230V 50Hz 8A FLA CEE 7 7 When the required electrical connection is available plug the provided power cord into the unit and the electrical outlet Cell Roller Ready Models 7402 amp 7403 These models have a cell roller a reinforced floor cell roller ramp and interior outlet These models were desi
30. bs 900 Ibs 825 Ibs 900 Ibs 374 kg 408 kg 397 kg 454 kg 374 kg 408 kg 374 kg 408 kg Specifications are subject to change without notice Environmental Conditions Temperature 15 C to 25 C Humidity non condensing Add 2 75 inches for handle heludes export shipping crate a 6020 Series units have forced internal air flow of 350 cfm 9 900 LPM 6040 Series units have forced internal air flow of 450 cfm 13 000 LPM 7400 Series Operations Manual Rev B 02 24 15 Page 82 of 88 page intentionally left blank 7400 Series Operations Manual Rev B 02 24 15 Page 83 of 88 SECTION 14 ELECTRICAL SCHEMATIC MEL EA r A ae ii EINE LER LW DE Nos ilt EF THp Fa it m mi len Eu Lam va ral ad I a Frima idr X j 9 4 jm e a LA HE E IL an gt l HE CLE Erie CPTI TED MEENE GUB RATE P POSE caus ih 11 T ne m A ta ua nA iai atte uM Tw M INIT ARA ae Ex de ij lei SECTION 15 TROUBLESHOOTING Problem Unit will not turn on e s the unit connected to a dedicated electrical circuit as defined in the installation section of the manual e 5 there power at the electric outlet the unit is plugged into e s the units power switch turned on
31. by opening the hinge and snapping it securely around the metal pen arm Refer to the image below PEN CAP k eee PEN CARTRIDGE Pen Arm Calibration To check and or adjust the recording pen s calibration to the outer most temperature graduation of the chart press and hold the change chart button 3 until the pen begins to move off of the chart Once the pen s has moved off of the chart again press and hold the change chart button 3 until the pen begins to move back onto the chart The pen should briefly stop at the outer most temperature graduation of the chart before continuing onto the chart to begin recording If the pen does not stop exactly at this location on the chart it can be adjusted or calibrated by using the left 1 or right 2 arrow buttons When the pen moves back onto the chart and briefly stops you will have approximately five seconds in which to adjust the pen s position using the left and right arrow buttons of Figure 3 On multiple pen recorders each pen will move one at a time onto the chart briefly stopping at the outer most temperature graduation of the chart at which time the pen s position can be adjusted by using the left 1 or right 2 arrow buttons When the time to adjust the position of the first pen has expired the second pen will move onto the chart briefly stopping at the outer most temperature graduation of the chart at which time the second pen s position may be adjusted E
32. capable of supporting a uniformly distributed load of 50 16 The maximum chamber capacity is 500 pounds stationary Optional shelving is available for supporting heavy loads such as shakers or cell rollers Chamber should be empty when being moved A A Do not pull multiple loaded shelves out simultaneously or the chamber may tip Models 7401 and 7403 do not have sliding shelves but a sliding shelf accessory can be purchased 7400 Series Operations Manual Rev B 02 24 15 Page 13 of 88 Leveling the Unit Place a level on the middle shelf of the incubator Adjust the feet until the unit sits level left to right and front to back Even if the unit is level without adjustment the leveling feet should still be lowered to avoid the cabinet moving while opening and closing the outer door amp prevent a flat spot from forming on the casters Connecting the Drain to connect the unit to a drain could result in facility flooding A When using a pressurized water source failure The incubator can have humidity either by an evaporative pan or injecting water To remove excess water that is not humidified or water that can be spilled when filling the evaporative pan connect the drain fitting and tubing supplied with the incubator to a local floor drain minimizing the risk of contamination and corrosion from the chamber The drain line must remain O The drain line relies on gravity to remove water below the chamber to drain
33. ce instruments CO2 sensor in close proximity to the cabinet s geometric center Be sure that the stabilization times described earlier have been satisfied prior to performing this calibration A positive value will move the humidity up and a negative value down Press the home button and verify the proper humidity is displayed 7400 Series Operations Manual Rev B 02 24 15 Page 55 of 88 SECTION 8 ALARMS Alarm System Overview The incubator control system is equipped with an alarm system that constantly monitors temperature CO2 and humidity on controlled humidified models to ensure the user is notified if the cabinet goes into an alarm condition Notification occurs via an alarm pop up window and a buzzer Each alarm condition has been factory programmed to minimize nuisance alarms while maximizing warning time There is a 2 hour time delay at start up and set point changes avoid nuisance alarms after a routine door opening an alarm condition must be present for 15 minutes before the operator is alerted If the optional remote alarm contacts are present in an alarm condition the dry contacts will change state The following alarm messages could be displayed Chamber temperature is higher than set point temperature Chamber temperature is lower than set point temperature Chamber CO2 is higher than set point CO2 Chamber 2 is lower than set point CO2 Temperature sensor error In the event an alarm occurs the alarm ind
34. e from chemicals or cleaning agents detrimental to equipment materials Force Majeure or Acts of God 7400 Series Operations Manual Rev B 02 24 15 Page 7 of 88 This writing is a final and complete integration of the agreement between CARON and the customer CARON makes no other warranties express or implied of merchantability fitness for a particular purpose or otherwise with respect to the goods sold under this agreement This warranty cannot be altered unless CARON agrees to an alteration in writing and expressly stated herein shall be recognized to vary or modify this contract Ohio Law governs this warranty Caron Products amp Services Inc PO Box 715 Marietta OH 45750 740 373 6809 7400 Series Operations Manual RevB 02 24 15 Page 8 of 88 INTERNATIONAL SYMBOLS AND DEFINITIONS Help Information Warning of hazardous area Warning of hot surface Warning of dangerous electric voltage opPp poo Earth ground protective conductor WARNINGS 5 Local government may proper disposal 7400 Series Operations Manual Rev B 02 24 15 Page 9 of 88 SECTION 2 EQUIPMENT OVERVIEW Congratulations You have just purchased the latest technology in CO2 incubators Before using the equipment familiarize yourself with key components of the product and thoroughly read this manual 7 Full Replaceable air P Color HMI intake filter a refrigerated Eis Right side Outer
35. e is displayed Temperature calibration example 7400 Series Operations Manual Rev B 02 24 15 Page 54 of 88 If the chamber temperature display reads 40 0 C and the calibrated independent sensor shows 40 3 C set the temperature offset value to 0 3 C If the calibrated independent sensor shows 39 6 C then the entered offset should be negative In this example the required offset to temperature would be 0 4 C Calibrating the Humidity Only Controlled Humidity will be displayed on the touchscreen and can be calibrated If humidity calibration is needed the following steps can be taken Locate the reference instruments humidity sensor in close proximity to the cabinet s geometric center Be sure that the stabilization times described earlier have been satisfied prior to performing this calibration A positive value will move the humidity up and a negative value down Press the home button and verify the proper humidity is displayed Humidity calibration example Only Controlled Humidity will be displayed on the touchscreen and can be calibrated If the chamber temperature display reads 80 and the calibrated independent sensor shows 83 set the humidity offset value to 3 0 If the calibrated independent sensor shows 74 then the entered offset should be negative In this example the required offset to humidity would be 6 0 Calibrating CO2 If CO2 calibration is needed the following steps can be taken Locate the referen
36. e pressure should be regulated to not exceed 80 psi CRSY102 or BOTL301 are accessories that can be used as a water supplies If a Condensate Recirculator CRSY102 water recycling system was purchased as a water supply refer to its user s manual for proper installation of the water supply 7400 Series Operations Manual Rev B 02 24 15 Page 19 of 88 Connecting Analog Outputs OUTP302 OUTP303 With the purchase of OUTP302 or OUTP303 the controls are eguipped with analog outputs OUTP302 provides 2 connections for monitoring temperature and humidity or CO2 OUTP303 provides 3 connections for monitoring temperature humidity and 2 Analog outputs are either milliamps 4 20mA or voltage 0 5V signal output that represents each of the displayed temperature humidity or CO2 values These options can be used for connection to in house data acquisition recorder or alarm system Analog Output Corresponding Value 4 20 mA 0 100 Humidity 4 20 mA 0 100 rh 4 20 mA 0 100 Connect shielded wires to the appropriate signal terminals l 4 for current mA or V for voltage DC For both current and voltage outputs COM is common terminal Analog Output Connections Back of unit 7400 Series Operations Manual Rev B 02 24 15 Page 20 of 88 Installing Drain Water Pump PUMP301 Pump Outlet to Sink or Floor Drain Pump Inlet from Chamber Drain Heservoir with Internal Level Switch In app
37. en Alarm Contact connections 7400 Series Operations Manual RevB 02 24 15 Page 17 of 88 Installing Carboy water system BOTL301 Incubators can be purchased with an optional 2 5 gallon carboy water system The carboy system is preassembled and shipped inside the chamber The four bolts required to mount the carboy to the unit will be mounted in the left hand side of the chamber Remove the carboy assembly from inside the chamber and attach it to the chamber using the 4 bolts Va Bolts 4 total Tubing connects disconnect to carboy Attach the preassembled tubing provided with the carboy to the water inlet on the rear of the chamber Tubing to water inlet Fill the carboy with water as described in the Using the Carboy Water System section of the manual 7400 Series Operations Manual Rev B 02 24 15 Page 18 of 88 Connecting to Humidity System HUMD304 HUMD307 Use only distilled or deionized water with a resistivity between 50KQ CM and 1MQ CM and a pH of greater than 6 5 Using water outside this range will void your warranty Do not use water that contains chloramines Chloramines can damage internal rubber gaskets resulting in leaks If these water sources are not available contact CARON customer service A water inlet fitting on the back of the unit and 4 black tubing are provided to connect the water supply to the incubator Connect an appropriate water supply to the fitting Incoming lin
38. ery company In the event of concealed damage after the unit is uncrated keep the carton and packaging material Call the shipping company within 7 days of receipt request inspection and retain a copy of the inspection report Caron provides full on site installation services for all models Our installation services guarantees the proper set up and startup of all equipment Please contact the Service Department at 740 373 6809 or service caronproducts com for details Choosing a Location This product weighs in excess of 500 pounds Ensure that sufficient resources are available to safely move the product To ensure proper operation the unit must be located on a firm level surface capable of supporting approximately 800 pounds The unit should be located in an 18 25 C ambient area and where there is no direct airflow from heating and cooling ducts as well as out of direct sunlight Allow four inches of clearance on all sides of the product to allow for connections and airflow The unit requires a dedicated electrical connection Depending on the options purchased with unit a floor drain may be required Choose a location where these facilities are or can be made available If a water source or a drain is not available contact CARON customer service and ask about our CRYS102 product line or click this web link for information on the product http www caronproducts com 65 Preliminary Cleaning 7400 Series Operations Manua
39. ess shipment of equipment or parts e Any customer modifications of this equipment or any repairs undertaken without the prior written consent of CARON will render this limited warranty void e CARON is not responsible for consequential incidental or special damages whether shipping damage or damages that may occur during transfer to the customer s point of use When the equipment is signed for at the customer s site ownership is transferred to the customer Any damage claims against the shipping company become the responsibility of the customer e Hepairs necessary because of the equipment being used under other than normal operating conditions or for other than its intended use Repair due to the customer s failure to follow normal maintenance instructions Parts considered consumable including light bulbs filters gases etc Damage from use of improper water quality Damage from chemicals or cleaning agents detrimental to equipment materials Force Majeure or Acts of God This writing is a final and complete integration of the agreement between CARON and the customer CARON makes no other warranties express or implied of merchantability fitness for a particular 7400 Series Operations Manual Rev B 02 24 15 Page 5 of 88 purpose or otherwise with respect to the goods sold under this agreement This warranty cannot be altered unless CARON agrees to an alteration in writing and expressly stated herein shall be recognized to vary or m
40. f the chart allowing you to place the recording chart paper onto the recorder Press and release the Change Chart button to begin recording 7400 Series Operations Manual Rev B 02 24 15 Page 49 of 88 Changing the Chart Paper Press and hold the Change Chart button 3 for approximately one 1 second until the pen begins to move off scale and then release the button Note The green LED light will flash fast while the thermal pen arm is moving off scale Wait until the thermal pen arm has moved completely off scale and stops the green LED light will stop flashing and will be steady On To remove the chart paper unscrew counter clockwise the chart hub knob at the center of the chart Gently lift the thermal pen arm just enough to be able to slide the paper out from beneath it Remove the old recording chart paper and position a new one He attach the chart hub knob and screw securely by hand against the chart Press and hold the Change Chart button 3 again for approximately one 1 second and the thermal pen arm will move back onto the chart and begin recording Green Light LED Status The green LED light located just below the three button membrane switch is used to show the recorder s status 1 LED on steady not flashing and input s recording within chart range indicates unit is recording normally 2 LED on steady not flashing and pen arm above outermost graduation and not moving indicates recorder is in Cha
41. gned to hold a 3 5 or 7 tier Wheaton R2P cell roller oupport braces have tracks to guide the cell roller wheels into the incubator The cell roller ramp has small tabs at the end that fit into an opening at the end of each cell roller support brace The ramp is designed to support the weight of the cell roller as it is pushed into the incubator Once the cell roller is pushed into the incubator remove the ramp and save it in a convenient place should it need to be reused to remove the cell roller handle Ensure that sufficient resources are available to safely move the product A Cell Rollers can be heavy and awkward to 7400 Series Operations Manual Rev B 02 24 15 Page 16 of 88 SECTION 4 OPTIONAL ACCESSORY INSTALLATION Connecting Alarm Contacts ALRM302 With the purchase of ALRM302 a set of terminals on the rear of the unit is provided to monitor temperature and humidity controlled humidity option alarms With the alarm contacts the terminals provided allow for a NO normally open output a NC normally closed and COM common connection In the event of an alarm condition or power failure the NO contact will close and the NC contact will open Once the alarm is cleared the contacts return to their normal conditions Insert the appropriate wire into the terminal and tighten down the screw terminal on top of the connector Terminal Connection Unit off Normal Alarm N O to C Closed Open Closed N C to C Open Close Op
42. hamber is equipped with those features All data is time stamped with year month day of the month hour minute 24 hour time ISO 8601 format This data is stored internally in the chamber in non volatile memory Data is logged every 5 minutes provided the chamber is on more than 10 years of data can be stored in memory If the internal memory fills up new data overwrites the oldest data Continuous writing to the flash drive necessitates a high quality industrial grade device Use only the flash drive provided by Caron or equivalent single level cell memory wear leveling algorithms error correcting code File name format is Data start YYYY MM DD HH MM hours in 24 hour time When the chamber is on the chamber s history data is being stored even when a flash drive is not inserted in the USB port This data may be retrieved anytime using the provided USB flash drive Here are the methods for retrieving data Continuous logging of data Insert the flash drive into the chamber s USB port When first inserted it creates a csv file called DATA START with the current date and time in the file name At 5 min intervals the chamber s process values are appended to the file The file will get as large as the flash drive will allow which would be years of data 7400 Series Operations Manual Rev B 02 24 15 Page 36 of 88 We 9 10 DEGA USB icon USB icon appears in in Status bar indicating that data is being wri
43. hart paper Gently lift the thermal pen arm just enough to be able to slide the paper out from beneath it Hemove the recording chart paper and place the Setup Chart onto the recorder This chart contains the configuration categories of the recorder Probe Input Inner Chart Temperature Outer Chart Temperature Temperature Scale Chart Rotation Speed Input Filtering Optional Relay Contacts and Date Time for internal clock Next press and hold either button 1 or 2 until the green LED light goes out and release the button If this step is successfully completed the pen arm will move to the outermost graduation ring of the Setup Chart Use the Left 1 or Right 2 arrow buttons to adjust the center of the thermal pen to be on this outermost graduation ring Position the Setup Chart so that the tip of the thermal pen is in the center of the Start circle Tighten the chart hub knob to secure the chart in place Next press and release the Change Chart button to begin The chart will rotate to the first category Input 1 Use the Left and Right arrow buttons to move the thermal pen arm to the desired option of each category Press and release the Change Chart button to accept the selection and advance to the next category You must press and release the Change Chart button when you have finished configuring the last category in order to save all of the changes that have been made to the recorder s configuration The thermal pen arm will move off o
44. hat all reference instruments are calibrated to an appropriate standard The Calibration Screen To get to the calibration screen from the home page Settings Button i a 5 3 0 90 Settings 279 Main screen with HUMD304 HUMD307 option Settings KE Press the Settings button 7400 Series Operations Manual Rev B 02 24 15 Page 52 of 88 Calibrate Button We 9 10 5 4 Alarms a Calibrate A Password 7 D Maint D Setup Factory na Calibrate Once the settings screen appears press the Calibrate button 1 Settings Temperature cabrae FA Calibrate Button Humidity 2 Calibrate Calibrate Button Button 7400 Series Operations Manual Rev B 02 24 15 Page 53 of 88 Calibrating the Temperature If temperature calibration is needed the following steps can be taken Locate the reference instrument s temperature sensor in close proximity to the cabinet s geometric center Be sure that the stabilization times described earlier have been satisfied prior to performing calibration 1 Home Settings xx gt Calibrate gt Temperature Calibrate Button ill i il i Enter the temperature offset by using the keypad and pressing Enter when complete A positive value will move the temperature up and a negative value down Press the home button and verify the proper temperatur
45. icator will appear on the status bar and an audible alarm will occur The flashing Alarm icon will appear on the status bar and the alarm pop up window will automatically appear Audible a Alarm icon The flashing Alarm icon will appear on the status bar 7400 Series Operations Manual RevB 02 24 15 Page 56 of 88 ll Alarm Screen Alarm condition Temperature Low Alarm condition Close Window Snooze Button Close Window Snoozing the speaker When in an alarm condition the speaker can be temporarily silenced to avoid being a nuisance to those nearby The alarm will reappear after 60 minutes has passed if the condition has not been corrected The audible alarm will not sound if the alarm speaker is muted Snooze Press the Snooze button the audible alarm is silenced for a period of 60 minutes When the alarm condition is corrected the alarm indicator and the audible alarm will automatically turn off unless there is another alarm condition To check what the alarm condition is press the Alarm button on the status bar and the alarm window will be displayed If the Snooze button has already been pushed and 60 minutes have not passed the Snooze button will be greyed out Close Window If you press the E Close Window button the Alarm Window will close but the alarm will still be present as a flashing alarm icon on the s
46. l Rev B 02 24 15 Page 12 of 88 Your new incubator was thoroughly cleaned prior to leaving the factory It is recommended however to disinfect all interior surfaces with a general purpose laboratory cleaning agent prior to using the product After cleaning dry all interior components with a sterile cloth as necessary Installing the Port Stoppers The unit has an access port built into each side of the cabinet The ports are designed to allow customer access for equipment validation or for installation of other equipment inside the chamber These ports should be sealed with the provided rubber stoppers to allow the incubator to function properly Install the stoppers provided in the port on each side of the unit Installing the Shelves Models 7400 25 33 incubators include sliding perforated stainless steel shelves Each shelf requires two shelf channels for installation Prior to installation take time to consider what the size of the product being placed in the incubator will be and set the shelf spacing accordingly Additional shelving can be purchased through CARON customer service if necessary To install the shelf channels slide on end of the shelf track into the appropriate sheli location in the rear plenum Hook the front of the shelf track into the corresponding location on either the right or left side front shelf support pilaster Repeat the process on the opposite side of the incubator Shelf capacity each perforated shelf is
47. l Rev B 02 24 15 Page 59 of 88 Changing Alarm Set points All alarm set points were pre set at the factory to minimize nuisance alarms that could be created as a result of door openings Alarm set points can be changed based on individual user requirements To change the alarm set points We 4 10 JEN Settings Button Settings ed Press the Settings button Alarms Button p 4 Setup Temperature Alarm Button Humidity Alarmi Speaker Mile Humidity Alarm Button a Temp Alarm Press the Temp Alarm button 7400 Series Operations Manual Rev B 02 24 15 Page 60 of 88 4 Home A Temperature Alarm High Limit A 75 Settings 5d Alarms A Y Temperature mil Alarm Low Limit Keypad screen will appear Enter the High Temp Alarm value press 3 Enter when complete Alarms To change the CO2 Alarm press the Alarms button on the navigation Alarm menu to go back to the Alarms screen Press the CO2 Alarm and Humidity Alarm Humidity Alarm Controlled Humidity only buttons and repeat the same steps for CO2 and humidity 7400 Series Operations Manual Rev B 02 24 15 Page 61 of 88 SECTION 9 ALERTS Alert System Overview The incubator control system is equipped with an Alert system that constantly monitors features of the incubator and to notify the user if the incubator needs any type of service that
48. lications where a floor drain is not available and a CARON water recycling system CRSY102 is not being used a drain pump can be purchased to pump any excess condensate from the chamber to a local sink or drain The pump is located near the middle of the back of the chamber Connect the supplied tubing from the pump to the sink drain The tubing may be run vertically into a ceiling but should not exceed 15 feet height The pump is equipped with a small reservoir on the bottom of the pump with an internal level switch that will automatically turn the pump ON when it is full to drain the water out of the reservoir and into a floor or sink drain 7400 Series Operations Manual Rev B 02 24 15 Page 21 of 88 SECTION 5 OPERATION With the chamber properly installed and the appropriate utilities connected the power switch on the right side top wrap can be turned on Within a few minutes the temperature and humidity will begin to approach set points Here is an overview of the home screen Status Bar Actual Day Time Actual Humidity Temperature Tu 10 35 AM af T Home e opeaker Setpoint 79 Setpoint P es Menu Lockout 37 s Y OQ Settings s Settings icon Parameter Display Zones D Main screen with HUMD304 HUMD307 option 7400 Series Operations Manual Rev B 02 24 15 Page 22 of 88 Using the Keypad This control system uses a numeric keypad to enter all parameter values Similar to a calculat
49. llow these simple rules 1 Never let the thermal pen tip ride on the chart plate when the chart paper is not present This will damage the protective coating of the heating element 2 Never use chart paper that is creased or that has been folded 3 Periodically clean the thermal pen tip with a cotton swap dipped in alcohol Clean more often when operating the recorder in a dusty environment 4 Always keep the door closed while the unit is recording 5 Never lift the pen arm more than is necessary to remove and replace the chart paper Excessive lifting may cause a decrease in the pen tip pressure and cause light printing 7400 Series Operations Manual Rev B 02 24 15 Page 51 of 88 SECTION 7 CALIBRATION The temperature and humidity systems can all be calibrated as necessary CARON recommends an annual calibration check of each system Before making a calibration adjustment allow the cabinet to stabilize a minimum of 12 hours from a power off condition 1f the unit has been in operation allow a minimum of 3 hours of stable operation at all set points If you do not have the appropriate reference instruments to perform calibration contact CARON s service department for on site calibration at service caronproducts com Caron also provides validation services which ensures that the unit is functioning properly according to IQ OQ and PQ protocols which satisfy FDA guidelines for qualification verification of equipment PA Be sure t
50. matically enter screen saver mode after 15 minutes At this time the screen will be completely blank ie black The illuminated Caron logo see Equipment Overview section shows that the unit is powered on and functioning wake up the touchscreen simply press anywhere on the touchscreen and the main screen will display If the unit has an alarm condition the touchscreen will not go into screen saver mode If an alarm condition occurs while in screen saver mode the display will automatically wake up and display the alarm 7400 Series Operations Manual Rev B 02 24 15 Page 24 of 88 Changing the Temperature Set point The steps below walk through an example ot changing the temperature set point from 37 0 to 20 0 This example shows optional humidity control Here is the display of the home screen Actual Tu 10 35 Setpoint Button Temperature Sf 2 0 90 Settings 5 0 1 2 setpoint ES To set the temperature set point press the Setpoint button on the right side of the screen Temperature Setpoint Button 37 0 Once the Setpoint screen appears press the Temperature Setpoint button In this example the temperature set point initially has a value of 37 0 this will vary with different initial set point values 7400 Series Operations Manual Rev B 02 24 15 Page 25 of 88 Keypad A temperature setpoint window will appear Enter the temperature setpoint by using the
51. nge Chart mode Press and release Change Chart button to return to normal recording mode 3 LED flashing rapidly and one or both inputs recording at outermost or innermost graduation indicates a sensor break Check or replace sensor s If sensor s are ok make sure process temperature is within configured range of recorder 4 LED flashing slowly 8 seconds ON 8 seconds OFF indicates recorder is in oet Up mode Refer to section CONFIGURING THE RECORDER 5 LED is Off indicates that there is no power to the recorder Check A C power to the recorder 7400 Series Operations Manual Rev B 02 24 15 Page 50 of 88 Recorder Calibration If calibration is required for single input recorders use the Left 1 and Right 2 arrow buttons on the recorder to calibrate the temperature being recorded on the chart to correspond to the temperature of the solution The arrow buttons must be held for approximately eight 8 seconds before the pen begins to move If calibration is required for dual input recorders you must first select the input that you wish to calibrate This is done by pressing and holding the Left 41 arrow button to select Input 41 or the Right 2 arrow button to select Input 42 The arrow button must be held down until the green LED light turns off after which follow the instructions in single input instructions above Maximizing Pen Life In order to maximize the amount of life expected out of the thermal pen tip fo
52. odify this contract Ohio Law governs this warranty 7400 Series Operations Manual Rev B 02 24 15 Page 6 of 88 EGUIPMENT INTERNATIONAL LIMITED WARRANTY Please review this section before reguesting warranty service At CARON one of our primary goals is to provide customers with high levels of personal service and top guality products delivered on time backed by technical service and supported for the life of the product Before contacting your distributor for warranty service please be aware that there are repairs that are not covered under warranty WARRANTY DEFINED Caron Products amp Services Inc herein after CARON hereby warrants that equipment manufactured by CARON is free from defects in materials and workmanship when the equipment is used under normal operating conditions in accordance with the instructions provided by CARON COVERED e Parts for a period of two 2 years from date of shipment e Any part found defective will be either repaired or replaced at CARON s or their authorized representative s discretion Parts that are replaced will become the property of CARON f CARON or their authorized representatives determine that the customer s unit requires further service CARON or the representative may at its sole discretion provide a service technician to correct the problem or require the return of the equipment to the an authorized service depot e CARON or their authorized representative will have the right
53. or this allows guick and precise entry of values When any numeric value button is pressed the keypad display will pop up over the current display Parameter Description Range of variable Header Keypad Display Escape Clear Negative sign Enter Decimal The Parameter Description Header tells what parameter is being changed The Keypad Display shows allowable values of the parameter being changed initially and displays the entered value when a button is pressed The Escape Esc button aborts the entry and returns to the previous screen without changing the value The Clear Clr button erases the value that you have entered After you have entered the value that you want pressing the Enter Ent button and the new value will take effect This also closes the keypad window Other keypad buttons include a decimal point button and negative button If an invalid numeric button is pressed such that it would create an entry above the parameter s range the entered number will not display For example if the temperature set point range is 5 0 to 70 0 pressing 8 followed by an 0 only the 8 will display If an invalid entry is made with an entry below the range such as a 4 followed by the Ent button then the entry will clear and the range will be re displayed 7400 Series Operations Manual Rev B 02 24 15 Page 23 of 88 Learning the screen saver To ensure long product life the touchscreen display will auto
54. ries Operations Manual Rev B 02 24 15 Page 63 of 88 We 3 10 AM a Reset button Maintenance items pe settings NM Replace CO filter Z 4 Rov Malni OA Calibration due Time left until service is required Once a Maintenance item is displayed on the Alert screen it will continue to be present as an icon in the Status Bar until the Maintenance item is corrected and the Reset Reset button is pressed resetting the replacement time to new status Press the Home button to return to the main screen 7400 Series Operations Manual Rev B 02 24 15 Page 64 of 88 SECTION 10 INFO Info System Overview The incubator control system is equipped with an Information system that constantly monitors the incubator and to notify the user when an automatic condition is occurring This applies to conditions such as Decon Cycle or others that cannot be switched on and off by the user but is controlled automatically by the software of the control system This notification cannot be disabled it only lets the user know the incubator s current status Notification occurs via an Info pop up icon on the status bar When the Info icon is pressed a pop up window will display the Info condition s Info Button Info Screen Decon Cycle Info Close Window Press the Close Window button to return to the main screen 7400 Series Operations Manual Rev B 02 24 15 Page 65 of 88 SECTION 11 ADVAN
55. rm Set points Section 9 Alerts 62 Alert System Overview Maintenance Section 10 Info aka eb kh 65 Info System Overview section 11 Advanced Features tacui akne 66 oetting the time amp day Locking the controls Changing the Passcode Factory menu amp troubleshooting Section 12 Preventative Maintenance 79 Secllon 13 SpecifICatlons Lu D Ceo pP 81 Section 14 Electrical Schematics 22 84 Section 15 TroubleshooUNng vasi ra Coa Eis eei EXE ERR 85 Section 16 Spare Replacement Parts 86 Appendix A Declaration of Conformity 88 7400 Series Operations Manual Rev B 02 24 15 Page 4 of 88 SECTION 1 WARRANTY INFORMATION CO INCUBATOR LIMITED WARRANTY Please review this section before reguesting warranty service At CARON one of our primary goals is to provide customers with high levels of personal service and top guality products delivered on time backed by technical service and supported for the life of the product Before contacting us for warranty service please be aware that there are repairs that
56. tatus bar for the remainder of the 60 minutes time It will not reset the 60 minutes alarm countdown time if the alarm condition is viewed on the pop up window After the 60 minutes time has passed for an alarm condition the counter will reset itself to 60 minutes and repeat the countdown process again until the alarm has been resolved 7400 Series Operations Manual Rev B 02 24 15 Page 57 of 88 Muting the speaker By factory default when an alar condition is present the speaker will sound This speaker can be muted in an on off fashion eliminating all audible sounds Muting the speaker will silence it until manually un muted This is different than snooze in that fact that snooze can only be enabled when an alarm condition is present and only lasts for 1 hour When the speaker is muted the alarm icon continues to flash and the remote alarm contacts optional remain in the alarm state To mute the speaker Tu 10 35 AM 14 s Settings Button SHOUT OU Settings xE Press the Settings button k n Setpoint 4 S RASTI dep mad e Speaker Mute Alamis a b u tto n Speaker Mute Press the EE Speaker Mute button 7400 Series Operations Manual Rev B 02 24 15 Page 58 of 88 We 9 10 4 Humidity Alarm id Speaker Mute une Ww button On Speaker ur indicator changes to MM Speaker Muted icon 7400 Series Operations Manua
57. the incubator the touchscreen can be locked out The passcode is required to lock out the controls and the same passcode is used to un lock it The factory default passcode is 1234 This passcode can be changed by the user to create a unique 4 digit passcode There is also a feature that will let you change the passcode from the factory default to a user defined passcode To lock the touchscreen Tu 10 35 AM 4 Settings Control Lock Button Icon unlocked 37 els 4 Settings Press the Settings button zm i 7 A Settings Ax Password ais J nd 6 Button S Password Press the Password button 7400 Series Operations Manual Rev B 02 24 15 Page 69 of 88 We 9 10 Home 45 Lockout Keypad is Unlocked Passcode must be entered to Keypad jaki lock To change passcode enter Change current passcode followed Passcode MN by new passcode Press the ent Enter digits 1 2 3 4 press a Enter when complete The screen will change back to the Home Screen and the Control Lock icon will change to the locked position 7400 Series Operations Manual RevB 02 24 15 Page 70 of 88 We 4 10 Control Lock Icon locked When any button is pressed on the home screen the following pop up window will appear If the button is pressed the screen will change back to the Home Screen
58. trical Power Cell Roller Reinforced Shelf Section 4 Optional Accessory Installation 17 Connecting Alarm Contacts ALRM302 Installing Carboy water system BOTL301 Connecting to Humidity HUMD304 HUMD307 Connecting Alarm Contacts OUTP302 OUTP303 Install a Drain Water Pump PUMP301 Section 5 Operation 1 1 znana nana nana 22 Using the Keypad Learning the Screen Saver Changing the Temperature Set point Changing the Humidity Set point Changing the CO2 Set point Section 6 Optional Accessory Operation 31 Carboy water system BOTL301 Decontamination Cycle DECN301 Operation of the Data Logging System DLOG301 Using the Extended Temperature Range EXTD302 Built in Gas Guard System GASG302 Ultraviolet Germicidal Lamp LGHT602 Interior Electrical Outlet OUTL305 OUTL320 Built in Temp or Temp Hh 6 Pen Recorders RCDR316 RCDR31 7 Built in Temp or Temp Rh 10 Thermal Recorders RCDR318 RCDR319 7400 Series Operations Manual Rev B 02 24 15 Page 3 of 88 SECON 7 CallbFallol iieri cios ad ilka 52 Calibrating the Temperature Calibrating the Humidity Calibrating the CO2 8 e AIT EUN aaa RP a le EN EE ERR HEUS 56 Alarm System Overview Snoozing the Speaker Muting the Speaker Changing Ala
59. tten to flash drive To retrieve the data press the Eject button The flash drive is then inserted into a computer for uploading the data Upon re insertion of the flash drive a new csv file is created even if the old file is still present File name nomenclature is Data Start YYYY MM DD HH with hours in military time History Retrieval Auto Export We 9 10 LUCES Be Automatically export stored data logging to flash drive when first inserted into LISB port Export history data to flashdrive Otherwise will only export history data from last export date Eject the fashdrive from the USB select the Auto Export feature on the USB menu screen Insert the flash drive into the chamber s USB port A new csv file is automatically created on the flash drive with all the stored history data The file name nomenclature is Data End YYYY MM DD HH MM 7400 Series Operations Manual Rev B 02 24 15 Page 37 of 88 We 9 10 d 5 Be All Data Automatically export stored data logging to flash drive when first inserted into USE port Export all history data to Otherwise will only export history data from last export date Eject the flashdrive from the USB There is also an All Data feature to indicate if the upload should include all data since the unit has been used or just the history data since a flash drive was inserted last An Info button will appear in the stat
60. us bar warning the user not to remove the flash drive while the data is being uploaded Ihe length of time to upload the file will depend on the file size When the Info button disappears from the status bar press the Eject button to safely remove the flash drive Now the data can be uploaded to a computer for viewing Using the Continuous Logging of Data method nothing on the touch screen has to be setup However using the History Retrieval method of data will require going into the USB screen to select either the Auto Export or All Data buttons before inserting flash drive into USB port To select the Auto Export and All Data buttons Settings Button Press the Settings button 7400 Series Operations Manual RevB 02 24 15 Page 38 of 88 Setup Button Press the Setup button We 9 10 LE USB Button Day Time Press the USB button Wa 9 10 AM 4 8 5 Automatically export stored data lagging to flash drive when first inserted into USB port Export all history data to flashdrive Otherwise will only export history data from last export date Eject the flashdrive from the USE port All Data When the All Data button is selected this will retrieve all of the data from when the chamber was first turned on up to the current time when flash drive was removed from USB port 7400 Series Operations Manual RevB 02 24 15 Page 39 of 88 Auto Export a
61. x 27 D 1 D x 52 7 H x52 7 H 32 W x 27 D x 52 7 32 W x 27 D 81 3 cm x 81 3 cm x 68 6cm x 68 6cm x 133 9cm 133 9cm x 66 H x 66 H Sens 81cm x 69cm 68 6cm x 133 9cm Interior Dimensions 81cm x 69cm x 168cm 35 5 W x 35 5 W x 35 5 W x 33 3 DX da 9uDX 999 DUX 36 W x 33 Doo Dx 36 W x 33 Exterior 77 1 77 1 77 1 D x 90 H 77 1 D x 90 H Dimensions 90 2cm x 90 2cm x 90 2cm x 90cm x 85cm 90 2cm x 90cm x 85cm 84 6cm X 84 6cm x 84 6cm x x 229cm 84 6cm x x 229cm 195 8cm 195 8cm 195 8cm 195 8cm Exterior Cold Rolled Steel Powder Coated Construction CHEER 33 A 29 no 33 Cu Ft 25 Cu Ft 33 Cu Ft 25 Cu Ft 708 33 Ft P 708 Liters 934 Liters 708 Liters 934 Liters Liters 934 Liters Type 304 Perforated Stainless Steel Electropolishe Type 304 Shelf Type 304 Perforated Type 304 Perforated Perforated Stainless Steel Stainless Steel Stainless Electropolished Electropolished oteel Electropolished Construction d Shelf 29 25 W x 26 45 D 29 25 W x 26 45 D 29 25 W x 26 45 D Dimensions 74 3cm x 62 1cm 74 3cm x 62 1cm 74 3cm x 62 1cm Electrical MODEL 7400 25 7400 33 7401 25 7401 33 7402 25 7402 33 7403 25 7403 33 115V 60Hz 16A 525 lbs 600 Ibs 525 lbs 600 Ibs 525 lbs 600 Ibs 525 lbs 600 Ibs 238 kg 272 kg 238 kg 272 kg 238 kg 272 kg 238 kg 272 kg 825 Ibs 900 Ibs 875 Ibs 1000 Ibs 825 I

REACH-IN CO2 INCUBATOR OPERATIONS

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