76875 gel dryer.qxd
Contents
1. 13 EHE PP 14 Instrument Rm 14 Technical 14 ATOMS 15 Replacement 5 amp Accessories ro Fach Peau te tapas nae caasa Du Le a etapa tei 16 Winng Diagrami MEE 17 E 20 Safety Information Alert Signals N Warning A warning alerts you to actions or conditions that can cause personal injury Caution A caution alerts you to actions or conditions that can cause faulty results or product damage Note A note informs you that a special condition exists or that a general rule should be followed Purpose This manual applies to the FisherBiotech Gel Dryer It is written for those people who use and maintain the Gel Dryer and its accessories Safety Information Throughout this manual visual cues are used to help you identify important information Unpacking 0 Caution For proper operation and to ensure compatibility with your Gel Dryer use only those accessories and options provided by Fisher which are designed specifically for the system Use only Fisher factory replacement parts If you need parts for your Gel Dryer contact Fisher s Instrument Service Div2. F Fisher Scientific Gel Dryer Model FB GD 45 User s Manual 76875 6 Table of Contents Safety REI T M 3 milis ccm 3 else uri Ec on 3 Unpacking MEC 4 Heceivingrand Unpacking iii tee nette cti i ed e aiaa aeaaea rain eis ih a 4 RR 4 Set UP iiaa O 5 SEISCUNG 3 Location 5 i mme e DELE 5 OSU UD 6 7 Operation i e d E pecs lettere aii raid i RR 7 8 Approximate Drying TIMES EI RT 9 MEIST 10 LEE m 10 MFOUDISSMOOUMG E 11 Replacing the Cover Seal m 12 Removing the Cover Sgal srities 12 Installing the Cover 0 4 12 Replacing the Vacuum OSC 12 Removallof the Vacuurrn FOoSO u uicti ed cene e rd n te eue uere yo euer aw Va yr kun d 12 Installation of the Vacuum 13 Replacing
3. Place at least one sheet of moistened filter paper or chromatography paper cut slightly larger than your gel s on the selected gel sup port When using the support mesh alone two or three thickness of paper are recommended Using thicker paper will require fewer layers Center the filter paper on the Gel Dryer if drying only one gel Place the gel s on the filter paper and smooth out any air bubbles Place a porous membrane over the gel s and remove any air bubbles from between the gel s and the membrane Place the cover seal over the gel s and smooth out any air bubbles OPERATION Figure 2 1 Control Panel 7 Turn the temperature knob to the desired setting The Gel Dryer is capable of heating from ambient temperature up to 80 C Note If you wish to maintain a constant plat en temperature below 20 C place the Gel Dryer in a cold environment and turn the temperature knob to the white area below the 20 C mark Note When timer knob is turned the power indicator light illuminates to indicate that power is on The heater indicator light illuminates when the heaters are activated The heaters will cycle on and off once the set temperature is reached in order to maintain the set temperature The timer controls power to the heaters only When the set time expires only the heaters will turn off The vacuum must be switched off manually Releasing the vacuum before the gel is co
4. 230 V SPN 83188 Power Cord North America 15 188 37 A Vacuum Hose Connector 1 4 hose I D FB GD 20 Vacuum Hose Connector 3 8 hose 1 0 FB GD 21 Vacuum Hose Connector 1 2 hose I D Fb GD 25 Plastic Support Mesh FB GD 22 Porous Support Pad FB GD 23 Cover Seal FB GD 24 Accessory Items Description Fisher Catalog Number Vacuum Pump Fisher Maxima D2A 110 volt 50 60 Hz 01 057 2A Diaphragm Vacuum Pump 110 volt 50 60 Hz FB DVP 353 220 volt 50 Hz FB DVP 353A Liquid Trap FB GDT 45 iagram D iring 17 SISSVHO NIYIN VI3NVd IOHINOO N31 V 1d 21 ma gt Nore 9N 5 3ords 8 wx 7 Dum SWHO pe m SLLVM 096 ASLL C um 1 3univuadWal ma 1HM Rs TO L JHL 5 H3MOd i NHO V 0 ed wo Lum 096 ASLL Le See 4 uwi ona va Dow D gt 1 Dem L YH G IOH M O N HH 5 HOSNES 154 5 DM t 1 YAWIL f a TBA NHO T ed of 27 s fi v8 0 iw 2 5 W N Ld X18 1HM AH LN3 H3MOd TBA NHO SISSVHO WOLLOG Warranty Laboratory instruments and equipment manufactured by Fisher S
5. VACUUM PUMP COLD LIQUID GEL DRYER CONDENSATION PRETRAP TRAP Figure 1 3 Set up without a condensation trap GLASS BLEEDER VALVE DIAPHRAGM LIQUID GEL DRYER PUMP PRETRAP 0 Caution When using the porous support pad the plastic support mesh must still be in place on the bottom of the Gel Dryer platen in order for proper vacu um to be obtained The plastic support mesh and the porous support pad must each be used with its smooth side up Note DNA sequencing gels which are already adhered to a sheet of filter paper can be placed directly on the support mesh 0 Caution Failure to cover the gel with a porous membrane may cause the cover seal to stick to the gel which may result in tearing of the gel Operation Operation Procedure Roll the cover seal up or lay it back over the control panel to expose the platen of the Gel Dryer Select a support for your gel and place it in the platen of the Gel Dryer The plastic support mesh is sufficient for most gels and will allow for the shortest drying time Very soft gels may be imprinted by the support mesh unless more than one sheet of filter paper is placed under the gel Also some filter paper residue may build up beneath the support mesh but regular removal of this residue will prevent clogging of the system The porous support pad may be more desirable for very soft gels However drying time will be significantly longer especially for agarose gels
6. Y FITNESS FOR ANY PARTICULAR PURPOSE OR ANY OTHER WARRANTY EXPRESS OR IMPLIED EITHER IN FACT OF BY OPERATION OF LAW STATUTORY OR OTHERWISE The above warranty and the above obligations to repair replace or credit are complete and exclusive and the Company expressly disclaims liability for lost profits or for special indirect incidental consequential or exemplary damages of any nature whether attributable to contract warranty negligence strict liability or otherwise even if the Company has been advised of the possibility of such damages Representations and warranties made by any person including dealers and representatives of the Company which are inconsistent or in conflict with the foregoing warranty shall not be binding upon the Company unless reduced to writing and signed by an officer of the Company 2555 Kerper Boulevard Dubuque lowa 52001 Phone 800 926 0505 Fax 563 589 0516 www fishersci com
7. call your Fisher Scientific Service District Office Technical Service Fisher maintains a technical support organization which is available for help regarding use and maintenance of your Gel Dryer Our technical support group is staffed with courteous and competent personnel trained in the use of the Gel Dryer Fisher Technical Service Hot Line can be reached by dialing 1 800 388 8355 Specifications Line Voltage Line Frequency Power Consumption Dimensions Drying Surface Weight Temperature Control Timer on Heater Vacuum Pump Requirements Vacuum Hose Connectors 115 VAC 230 VAC 50 60 Hz 720 watts HxWxD 3 7 8 x 25 13 16 x 17 3 4 inches 9 8 x 65 5 x 45 1 centimeters 14 1 2 x 18 1 2 inches 36 8 x 47 centimeters 25 5 Ibs 11 6 kg ambient to 80 C Zero to five hours hold position for continuous operation Ultimate pressure 10 7 mm Hg Free air displacement 29 5 L min Accepts 1 4 3 8 or 1 2 vacuum hose Replacement Parts amp Accessories Replacement Parts Description Number Reference Fuses 115 V SPN 13775 F1 F2 230 V SPN 83105 F1 F2 Fuse Drawer 115 V SPN 76867 Fig 3 2 230 V SPN 83104 Fig 3 2 Power Input Block SPN 76866 Pi Timer SPN 76820 TMR Power Heat Indicator Light 115 V SPN 76865 PLI 250 V SPN 93106 PLI Heaters SPN 76821 HTR2 Thermal Fuse SPN 76868 TF Sensor SPN 76825 THM Temperature Controller 115 V SPN 83010 TC Temperature Controller
8. cientific Company L L C Laboratory Equipment Division hereinafter called the Company are warranted only as stated below Subject to the exceptions and upon the conditions specified below the Company agrees at its election to correct by repair by replacement or by credit to the purchaser any defect of materials or workmanship which develops within one year 13 months for refrigerator and freezer products from the date of purchase by the original purchaser by the Company or by an authorized dealer of the Company provided that investigation or factory inspection by the Company discloses that such defect developed under normal and proper use The exceptions and conditions mentioned above are the following a The Company makes no warranty concerning components or accessories not manufactured by it such as tubes batteries etc However in the event of the failure of any component or accessory not manufactured by the Company the Company will give reasonable assistance to the purchaser in obtaining from the respective manufacturer whatever adjustment is reasonable in the light of the manufacturer s own warranty The Company shall be released from all obligations under its warranty in the event repairs or modifications are made by persons other than its own service personnel or authorized dealer personnel unless such repairs by others are made with the written consent of the Company THE COMPANY MAKES NO WARRANTY OF MERCHANTABILIT
9. d Call Fisher Service Call Fisher Service Set temperature above ambient Turn timer knob past set point or 1 hour then to set point Call Fisher Service Call Fisher Service Call Fisher Service Reinstall cover seal Replace cover seal Check pump Check tubing for leaks Check tubing for clogs MAINTENANCE Replacing the Cover Seal If the cover seal becomes misaligned or damaged suffi cient vacuum may not be attainable The cover seal must be reinstalled or replaced to correct the problem Removing the Cover Seal A metal bar is used to hold the cover seal onto the platen To remove the cover seal perform the following proce dure 1 Remove the five Phillips head screws securing the metal bar 2 Remove the bar and lift off cover seal Installing the Cover Seal To install the replacement cover seal reverse the steps in the removal section taking care to align the holes in the cover seal with the holes in the platen Check to make certain the cover seal is aligned over the platen edges to insure proper vacuum seal Replacing the Vacuum Hose Connector The vacuum hose connector supplied with the Gel Dryer may be replaced if necessary for repair or to install anoth er vacuum hose connector for a different sized vacuum hose Removal of the Vacuum Hose To prevent damage to the vacuum hose connector use a 9 16 inch deep well socket for removal turning the con nector in the counter clockwise di
10. ision Plan the location of your Gel Dryer to allow for easy operation Protect against physical damage The Gel Dryer should be protected from dirt spray and airborne debris Ensure that the unit is set up to con firm to local regulations and within the guidelines for good laboratory prac tice 0 Caution When not being used the porous support pad and the plastic support mesh may be stored in the clear plas tic bag supplied with the packaging However to avoid damage to the Gel Dryer s surface never allow the clear plastic bag to contact the heated Gel Dryer surface Handle the Gel Dryer with care Do not drop or subject the unit to mechanical shocks Receiving and Unpacking the Gel Dryer Unpack the Gel Dryer and its accessories from the ship ping container Save this container for returning the unit in the event that service is required Check the cartons and components for signs of damage Check the packing list to ensure all parts are present Damaged or missing items should be reported to the shipping agent within 15 days If the Gel Dryer will not be set up immediately store the Gel Dryer and accessories in their original shipping con tainers until ready for installation The storage area should be clean and dry and should not subject the Gel Dryer to extremes in temperature Parts Supplied The following is a list of the parts and documents supplied with the Gel Dryer 1 power cord 3 vacuum h
11. it into the power source 4 Remove the plastic support mesh from the clear plastic bag and place it smooth side up on the platen of the Gel Dryer 5 Based on the inner diameter of the vacuum hose you intend to use select one of the two vacuum hose connectors Connectors are pro vided for 1 4 3 8 and 1 2 inside diameter hose 6 Thread the selected connector into the vacuum outlet on the left side of the unit in the clock wise direction For this purpose a 9 16 deep well socket is recommended 7 Connect the Gel Dryer to the vacuum source using thick walled vacuum hose SET Up For the cover seal to seal automatically the vacuum pump must be capable of achieving proper pres sure and flow see Specifications Use of a high vacuum pump such as the FisherBiotech diaphragm vacuum pump or the Fisher Maxima D2A will provide the fastest most consistent drying of your gels Caution 0 It is strongly recommended that both a liquid pretrap and a cold condensation trap be used to prevent liquid or water vapor from damaging the vacuum pump The condensation trap can be mechanically refrigerated unit or one can be constructed using a Dewar flask and a cold finger or a vacuum flask immersed in liquid nitrogen or packed dry ice See Figure 1 2 If a diaphragm pump is used a liquid trap alone may be sufficient to protect it See Figure 1 3 Figure 1 2 Set up with a condensation trap GLASS BLEFDER VALVE 4
12. mpletely dry may cause the gel to crack OPERATION 8 Use the timer control knob to turn on the power and set the desired heating time Turn the knob clockwise to set time for up to 5 hours or coun terclockwise to 00 for continuous operation 9 Turn on the vacuum source The cover seal will automatically seal The vacuum must be main tained until the gel is dry 10 Turn off the heat and allow the gel to cool before releasing the vacuum This will help minimize the dried gel 11 Lift the cover seal before turning off the vacuum source Approximate Drying Times Polyacrylamide Gels will dry in about one hour at the tem perature setting of 80 C when run with pumps specified in the accessory section Gels that are of higher concen tration than a standard 696 Acrylamide Gel may require longer drying times The drying time for these applications could be near 2 hours Lower temperatures can be used for thick or gradient Gels The drying time is reduced by having a good vacuum The normal vacuum is in the range of 10 40 mm Hg Maintenance N Cleaning Warning The platen of the Gel Dryer is coated with Teflon Use NEVER immerse the Gel Dryer in only mild soap and water and a soft cloth for cleaning it water Unplug the Gel Dryer before DO NOT use abrasive cleaners or scouring pads cleaning it 0 Caution Drying gels using only the plastic sup port mesh may cause a residue of filter paper dust to accumulate on the pla
13. ose connectors for 1 4 3 8 and 1 2 D hose 1 warranty card 1 porous support pad 1 plastic support mesh 1 user s manual Caution Ensure that the timer knob is set to 0 before plugging the Gel Dryer into the power source Never allow the clear plastic bag to come in contact with the heated Gel Dryer surface Caution The plastic support mesh must be in place in order for the Gel Dryer to perform properly 0 0 0 Caution Be careful not to over tighten the vac uum connector as this may damage the connector oet Up Selecting a Location The Gel Dryer should be set up on a level stable labora tory bench or cabinet Locate the Gel Dryer so that there is at least 10 cm 4 inches of clearance around the back sides and top of the Gel Dryer Select a location for your Gel Dryer which has an appro priate source of electrical power The power source must correspond to the data plate mounted on the rear of the unit Select a location away from solvent vapors and flamma bles where no explosion hazard exists The Gel Dryer must be connected to a suitable vacuum source for operation see Specifications Select a loca tion which will allow convenient access to the vacuum source Set Up Procedure 1 Remove the unit from the shipping carton and place it onto the laboratory bench 2 Plug the power cord into the Gel Dryer on the right hand side near the back of the unit 3 Plug the un
14. rection MAINTENANCE Installation of the Vacuum Hose Install the vacuum hose connector using a 9 16 inch deep well socket turning the connector in the clockwise direc tion To protect the connector from damage do not over tighten Replacing Fuses To inspect and or replace the fuses perform the following Figure 3 2 Power Input Block procedure 1 Remove the power cord from the wall outlet and from the socket in the power input block Locate the fuse drawer in the power input block See Figure 3 2 2 Insert a small flat bladed screwdriver or your fin gernail under the right edge of the fuse drawer and slide the fuse drawer out 3 Turn the fuse drawer over and you will see the fuses inside Pull the fuses out and inspect them If defective replace with fuses of same INPUT BLOCK type and rating only POWER 4 After replacing or reinserting the fuses slide the fuse drawer into the power input block until it snaps into place 5 Reinsert the power cord into the power input block MAINTENANCE Fisher Service Fisher Scientific actively supports the products it manufac tures and distributes Instrument Service Fisher Instrument Service representatives are skilled at troubleshooting and repairing the Gel Dryer Service is available at any of the Instrument Service District Offices located throughout the country For service or for informa tion regarding service contracts or parts Please
15. t en Wipe this residue out of the platen with a damp cloth on a regular basis to prevent clogging of the vacuum port Both the plastic support mesh and the porous support pad are made of polypropylene and may be damaged by exposure to certain organic sol vents They should be rinsed thorough ly with water after use and air dried If infectious agents are spilled on them they can be steam autoclaved to disin fect them Troubleshooting MAINTENANCE This table guides you in tracking down the cause of a problem based on a symptom description Symptom Power Indicator light does not illuminate Failure to heat Platen heats to 95 C then shuts down Platen heats but heater indicator does not light Cover seal does not seal Probable Cause Timer not set Power cord not plugged in Power cord not plugged into Gel Dryer Faulty or blown fuse Faulty power cord Timer defective Defective power indicator light Temperature not set above ambient Timer not set Electrical system failure Temperature controller failure Defective heater indicator light Cover seal misaligned Cover seal damaged Vacuum pump not functioning Vacuum tubing leaks Vacuum tubing clogged Recommended Action Turn timer knob past set point or 1 hour then set point Make sure power cord is plugged into wall outlet Make sure power cord is plugged into Gel Dryer Check fuse replace if necessary Replace power cor
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