Users Guide
Contents
1. Auto tuning When the system is assembled properly there is no need to run the Auto Tune feature However if the system becomes unstable due to changes in the microscope stage or Chamber the system might require tuning for optimal performance STEP 1 Assemble the unit properly power up the unit and make sure the CO2 supply is on and regulator is properly adjusted and that the water level is correct in the Humidification unit STEP 2 Enable the controllers with the Enable switches and allow the system to stabilize STEP 3 From the Front panel of each controller push the SEL button and hold it for 3 seconds or until the display looks like Auto Tune LED indicator STEP 4 Pushing the SEL button once more will display the value of the Auto Tune value When the system is normally operating the value is 0 In order to command the unit to self tune the 0 must be changed to a 1 by using the Up Arrow Once the display shows a 1 the SEL button should be held for three seconds until the display returns to the Set Value One more push of the SEL button will display the current reading During the Auto Tune process the Auto Tune LED will blink Calibration In the event the system requires calibration the displayed value can be offset using the PVOF menu Before entering the adjustment menu record the difference between the desired measurement and the displayed measurement STEP 1 This menu is acce2. Humidity Alarm Indicator Humidity Controller Humidity Enable Sw Humidification Bottle Assembly LiveCell Controller Unit P N 05 17 0000 REV 02 Functional Description page 2 Bottle Cap Assembly MAX Fill Line FILL Line MIN line Humidification Bottle Assembly Bottle Cap Assembly PIN 05 17 0000 REV 02 Functional Description page 3 Temperature Controller Temperature Enable Switch Temperature Alarm Indicator COQ Controller CO Enable Switch CO Alarm Indicator RH Controller RH Enable Switch RH Alarm Indicator Humidification Bottle Assembly Control Unit P N 05 17 0000 REV 02 The control unit for the Stage Chamber temperature Enables and Disables the Temperature control Illuminated is Enabled An LED that is illuminated any time the temperature differs from the Set Value SV by more than 0 5 C The control unit for the Stage Chamber CO concentration Enables and Disables the CO control Illuminated is Enabled An LED that is illuminated any time the CO level differs from the Set Value SV by more than 0 5 The control unit for the Stage Chamber humidity Enables and Disables the humidity control Illuminated is Enabled An LED that is illuminated any time the RH differs from the Set Value SV by more than 5 RH A bottle assembly used to introduce water vapor into the system The integrated controller for Temperature Humidity and
3. y Foam Sealing Strips P N 05 17 0000 REV 02 Lens Assembly CO gt z Sensor Inside COz Sensor Cable In From Controller Exhaust Front View of Incubation Chamber Foam Seal Connection for hose from LiveCell 3 Control Unit Exhaust Connection for hose to LiveCell 3 Control Unit Intake Sensor for CO2 level feed back to Controller Sensor for Temperature and Humidity feedback to Controller The connector for lid switch signals and temperature signal The connector to the CO2 sensor This switch disables controller outputs if the Chamber is not positioned properly The interchangeable optical window assembly for different illumination condensers Seals Chamber ato the Stage Functional Description page 7 General Capabilities Section 4 CO Control Inside Of The Microscope Chamber When the system is assembled properly it controls the CO level in the microscope chamber to the last entered Set Value The system uses a CQO sensor inside of the Chamber to provide information the CO Controller requires for precise and accurate stabilization of the COz level CO2 from the eternal source is regulated by the Controller in short bursts based on the self tuned calculations Front panel operations for the CO2 Controller are e Adjustment of the Set Value SV the desired COz level in the Chamber e Display of the Process Value PV the actual measured value of COz in the Chamber e Adjustment of the A
4. 0000 REV 02 General Capability page 2 Humidity Alarm LED When the humidity Process Value is higher or lower than the Set Value by the Alarm Threshold then the Red Alarm LED will light as a visual indication that a problem exists No audible alarm exists Only when Set Value Alarm Threshold lt humidity Process Value lt Set Value Alarm Threshold will the LED turn off Microscope Chamber The Microscope Chamber is designed to house various sizes and formats of incubation vessels as well as various working distance illumination lenses The accommodation of various working distance lenses is accomplished with removable drop in optical window assemblies The Chamber is designed to create a uniform flow across the optical axis for even control with minimal temperature gradients throughout the Chamber and the temperature humidity sensor is located in a region representing the average values For high humidity applications some condensation may occur in the corners or edges of the Chamber on the stage top or the Lens Assembly this is normal and primarily caused by slight temperature gradients in the chamber and ambient temperature The Chamber has a switch that will disable the CO2 and Temperature Controllers any time the chamber is repositioned This is intended to keep the system from over heating or filling the room with CQz in the event the chamber is not sitting properly on the stage while running and enabled Typical Usage The fo
5. CO2 Functional Description page 4 Intake from Chamber Exhaust to Chamber Power Entry CQ Entry Fuses On Off Switch Intake Exhaust Chamber Connector P N 05 17 0000 REV 02 Chamber Connector CO Connector Optional RS232 interface CO in from source On Off Switch Fuses a SSE I Power Entry Provides power to the whole system Provides CO to the system from an externally regulated source Two 250V 5A fast blow fuses type GMA 5ABC Turns the unit On and Off The return path for the air handling system This is connected to the Chamber s Output to Controller connection The output of the air handling system that goes directly to the Chamber s Input from Controller This is where the gray chamber cable connects to the Control Unit Functional Description page 5 CO2 Connector This is where the orange chamber cable connects to the Control Unit AN NOTE Trying to force the incorrect cable into a connector will result in bent or broken pins on the cable Optional RS232 This is were the RS232 cable would connect for optional serial communications with the controller P N 05 17 0000 REV 02 Functional Description page 6 Out to Controller Tntake Temp RH Sensor Chamber Cable Chamber Switch In From Controller Out To Controller CO Sensor Temp and RH Sensor Chamber Connector Sensor Connector Chamber Switch Lens Ass
6. cable into a connector will result in bent or broken pins on the cable STEP 4 Slide one end of a hose OVER the Chamber connection hub labeled IN FROM CONTROL UNIT S EXHAUST and the other end INTO the LiveCell 3 Controller Unit hub labeled Exhaust STEP 5 Slide one end of the other hose OVER the Chamber connection labeled OUT TO CONTROL UNIT INTAKE and the other end INTO the LiveCell 3 Controller Unit hub labeled Intake P N 05 17 0000 REV 02 Installation page 1 AN NOTE The hose connections should slide either into or over the hubs fully to prevent the hose from accidentally disconnecting STEP 6 Connect the COz source to the LiveCell 3 control unit and adjust the regulator to 10 psi Do not exceed 10 psi A NOTE When not in use the CO source should be disconnected and turned off STEP 7 If humidity is to be controlled remove the Humidification Bottle Assembly by pushing in on the humidification unit to release then removing Once removed the cap can be removed to add water to the bottle Add ROOM TEMPERATURE TAP WATER to the bottle s FILL line Once the cap assembly is re installed the water level will raise but should not be above the MAX fill line During operation make sure the level is within acceptable limits as displayed on the label DO NOT USE D I WATER D I water will disable the automatic shutdown of the humidification unit in the event of running out of water A NO
7. LiveCell Users Guide LiveCell _ Stage Top Incubation Table of Contents Introduction Warnings Functional Description General Capabilities Installation and Setup User Interface Section 1 Section 2 Section 3 Section 4 Section 5 Section 6 Introduction Section 1 The Neue Biosciences LiveCell 3 system is designed to provide users with a simple to use yet precisely controlled environment for the culturing of cells on a microscope stage You can adjust and precisely control the temperature humidity and CO2 level inside of the modest sized microscope stage chamber Accurate and precise closed loop control of CO eliminates the need for expensive pre mixed CO2 gas or the inconsistencies of flow rate mixing of gases to enrich the environment to the desired level Absolute control of the temperature and humidity levels in the chamber enables longer time lapse observations without worrying about evaporative loss or possibilities of condensation on the optical surfaces You can just simply adjust the temperature humidity or CO2 Set Points through a simple front panel interface enable the controllers and the control unit does the rest The microscope stage chamber is large enough to allow the user to view cells in larger culture flasks as well as slides and plates without suffering the poor regulation the need for a separate CO enrichment chamber and leaks common to the larger whole microscope chambers The LiveCell w
8. TE Do not attempt to loosen the screws on the cap A NOTE Do not overfill the bottle damage to the internal components may result P N 05 17 0000 REV 02 Installation page 2 User Interface LED that indicates the SET VALUE is currently displayed LED indicates that the controller is active Section 6 Label indicates the type of controller i e C CO2 or RH LED indicates alarm canditinn existe Select button used to select Down button to menu options navigate through menus and to modify data Up button to navigate through menus and to modify data Displaying Current Values Upon power up the controllers will display the current values for temperature humidity and CO0 and the SV LED will not be illuminated As the system stabilizes the current values can be observed Displaying and Setting the Set Values The previous Set Values are held in memory To change the Set Values the User must power the unit up wait for the displays to display actual numbers then press the SEL button once Now the SV LED will illuminate and the current Set Value will be displayed Use the Up and Down buttons to change the Set Value Once the Set Value is correct the User only needs to press the SEL button once to accept Three seconds later the value will be accepted stored in memory and the controller will begin to control the system to that value P N 05 17 0000 REV 02 Basic User Interface page 1
9. as designed to be a closed environmental control system in order to minimize the CO usage and therefore the leakage to the outside working environment this is particularly critical in small poorly ventilated labs OCCUPATIONAL LIMITS FOR AMBIENT C0 IS 5000 PPM THE LIVECELL 3SYSTEM HAS BEEN DESIGNED TO MINIMIZE CO LEAKAGE DURING NORMAL AND EVEN ABNORMAL CONDITIONS HOWEVER THE USER SHOULD INSTALL A CO2 MONITOR AND ALARM IN ANY AREA WHERE CO COULD BUILD UP TO DANGEROUS LEVELS The temperature is adjustable and controlled from ambient to 50 0 1 C The system only has heating capability humidity is adjustable and controlled from ambient to condensation within 1 and the C02 level can be adjusted and controlled from ambient to 10 0 1 This document is intended to be a comprehensive guide on the installation use troubleshooting and maintenance of the LiveCell 3 P N 05 17 0000 REV 02 Introduction page 1 Warnings Section 2 Proper Operation Proper operation of this equipment relies on the user reading this user guide in its entirety CO Use only in a well ventilated area or install a CO2 Monitor to ensure safety of the operator Disconnect the CO source when not in use Electrical Shock DO NOT OPEN THE CONTROLLER HOUSING FOR ANY REASON as there are no user serviceable parts inside and potentially dangerous voltages may be present even with the power cord disconnected Electrical Shock Use only properly grounded outlet
10. d accurate stabilization of the humidity Water vapor from the Humidification Bottle Assembly is injected into the system under regulation by the Controller based on the self tuned calculations IT IS IMPORTANT TO STABILIZE THE TEMPERATURE BEFORE ENABLING THE HUMIDITY SINCE RH IS TEMPERATURE DEPENDENT ALSO IT IS IMPORTANT TO BRING THE HUMIDITY LEVEL UP SLOWLY TO THE POINT THAT SOME CONDENSATION IS OBSERVED EITHER ON THE LENS ASSEMBLY OR THE STAGE TOP WHEN THIS OCCUS ADUST THE HUMIDITY SET POINT DOWN BY 5 AND THE CONDENSATION SHOULD DISSAPEAR IN A FEW MINUTES IF NOT ADJUST DOWN ANOTHER 5 The humidity drops very slowly and to speed the drop in humidity remove the hose at the Controller Intake Hub for a few seconds Front panel operations for the Humidity Controller are e Adjustment of the Set Value SV the desired humidity in the Chamber e Display of the Process Value PV the actual measured humidity in the Chamber e Adjustment of the Alarm thresholds the point at which the Alarm LED illuminates e Initiation of the Self Tune process a function of the controller to maximize the stability of the control algorithm All front panel operations of the controller can be accomplished through the optional RS232 interface as well Humidity Enable Switch The output of the humidity controller can be selectively enabled disabled by pushing the humidity Enable Switch The green LED in the switch turns ON when enabled P N 05 17
11. em with the Humidification Bottle Assembly removed for 15 minutes or until any condensation on the chamber disappears The bottle should be emptied when not in use stored or moved P N 05 17 0000 REV 02 General Capability page 4 Installation and Setup Section 5 Un Packing The following is a list of the packaged items 1 LiveCell Controller Unit 1 Power Cord 1 Gray 8 pin Mini DIN cable 1 Orange 8 pin sensor cable 1 Microscope Chamber 1 RS232 Cable optional 2 6 ft hoses 1 10 ft CO2 1 4 line 1 Optical Window Assembly Tube 1 Users Guide Installation The installation process is STEP 1 STEP 2 Install the Chamber on top of the microscope stage by tilting back the microscope s Illumination Pillar and positioning the Chamber over the stage until it sets completely over the stage and rests on the Foam Sealing Strips Note the click of the chamber switch as the chamber is lowered The Chamber can be installed with the connectors facing to the right or the left side but not front or back When properly installed the Chamber will be level with the microscope stage and should not hang over any edges Now the Illumination Pillar can be tilted back into position STEP 3 Connect one end of the 8 pin gray mini DIN cable to the connector labeled Chamber on the Chamber and the other end to the LiveCell 3 Controller Unit connector labeled Chamber A NOTE Trying to force the incorrect
12. larm thresholds the point at which the Alarm LED illuminates e Initiation of the Self Tune process a function of the controller to maximize the stability of the control algorithm All front panel operations of the Controller can be accomplished through the optional RS232 interface as well CO Enable Switch The output of the CO controller can be selectively enabled disabled by pushing the CO2 Enable Switch The green LED in the switch turns ON when enabled CO Alarm LED When the CO Process Value is higher or lower than the Set Value by the Alarm Threshold then the Red Alarm LED will light as a visual indication that a problem exists No audible alarm exists Only when Set Value Alarm Threshold lt CO2Process Value lt Set Value Alarm Threshold will the LED turn off Temperature Control Inside Of The Microscope Chamber When the system is assembled properly it controls the temperature in the microscope chamber to the last entered Set Value The system uses a thermal sensor inside of the chamber to provide information the Temperature Controller requires for precise and accurate stabilization of the temperature A heat source is regulated by the Controller based on the self tuned calculations Front panel operations for the Temperature Controller are e Adjustment of the Set Value SV the desired temperature in the Chamber e Display of the Process Value PV the actual measured temperature in the Chamber e Adj
13. llowing description is intended as a summary of the typical usage All of the following steps will be detailed later in this document STEP 1 The LiveCell 3 Controller Unit is turned on with the CO2 Humidity and Temperature Controllers Disabled After the Controllers initialize the Process Values will be displayed STEP 2 Check the Set Values by pushing the SEL button on the front panel of each controller To make sure the number displayed is the Set Value the SV LED should be illuminated In order to change the Set Point use the up and down arrow key to change the display to the desired Set Point Once the desired Set Point is displayed push the SEL button once more to accept the value The SV LED should turn off and the display will revert back to reading the Process Value STEP 3 Verify all connections are properly made STEP 4 Enable the desired controllers For example If only temperature control is desired then keep the COs and humidity disabled etc Allow the system to stabilize This will occur in less than 30 min The Alarm LEDs for the Enabled controllers will be off when stabilized STEP 5 Once stable the system is ready P N 05 17 0000 REV 02 General Capability page 3 STEP 6 Shutting the system down when humidity is not enabled is a simple as turning the power off and turning off the CO2 supply Shutting down the system when humidification has been used involves running the syst
14. o 50 0C For service or technical support contact Pathology Devices P O Box 1093 Westminster MD 21158 Phone 410 876 5004 Fax 410 876 6006 email sales pathologydevices com
15. on 3 The LiveCell 3 system is intended to control the temperature humidity and CO level within the Stage Chamber Thereby creating a long term incubation environment right on a microscope stage The system utilizes CO2from an external regulated CO2 source and then meters the COz into the system as a function of the measured concentration and the user selectable Set Point Value in order to maintain the concentration The system also humidifies and heats the circulating air as a function of the measured temperature and the user selectable Set Point Values in order to maintain the temperature and relative humidity The temperature humidity and CO2 concentration are all measured inside of the Stage Chamber in close proximity to the sample Enable switches have been provided to allow the user to run the system under temperature humidity or CO2 control alone all at once The Green LED of the switch will light when the controller is enabled In the event the Measured Value of CO concentration humidity or temperature fall outside of the allowable limits a red Alarm LED will light indicating a problem NOTE The Alarm LEDs will be illuminated during the initial startup cycle and will turn off when the system has stabilized OR when a controller has been disabled Humidity is actively added to the system if the water level is maintained within the limits displayed on the bottle holder A full bottle may provide up to 120 hours of hands off perf
16. ormance Great care has been taken to minimize the coupling of vibration from the Control Unit to the microscope however very high magnification may require the user to relocate the Control Unit if image quality is degraded The controllers are very sophisticated and have the ability to quickly bring the environment in the Stage Chamber to the Set Points and hold it there with tremendous stability The Set Point of the controllers can be easily changed at any time by pressing the SEL button then using the up or down arrow buttons to change the Set Point Pressing SEL once more changes the display back to the control mode and Measurement Value reading Specifications Supply Voltage 110VAC 5A 50 60 Hz Temperature Control Range Ambient 50 C Temperature Control Resolution 0 1 C Temperature Accuracy at 37 C lt 1 5 CO Control Range Ambient 10 CO Control Resolution 0 1 CQ Accuracy at 25 C lt 0 2 CQ Non Linearity lt 0 5 P N 05 17 0000 REV 02 Functional Description page 1 RH Control Range Ambient 85 RH RH Control Resolution 1 RH Accuracy at 37 C lt 2 Operational Temperature Range 25 35 C Operational Humidity Range 0 85 non condensing Warm up time for stabilization lt 30 min but depends on room temperature Storage Temperature 30 70 C Temperature Alarm Indicator Temperature Controller CO Alarm Indicator CO Controller Temperature Enable Sw CO Enable Sw
17. s Other Uses The Equipment is intended to be strictly dedicated to the control of the environment on the microscope stage that the chamber is specifically designed for Operation of the Equipment for any other purpose or microscope stage may result in permanent damage to the Equipment or may create a harmful situation for the user THE INSTALLATION ON ANY OTHER DEVICE MAY DAMAGE THE EQUIPMENT INJURE THE USER AND VOID ALL WARRANTIES Water for Humidification Use room temperature tap water to fill the bottle to the fill line DO NOT OVERFILL Overfilling the bottle may result in damage to internal components of the system DO NOT USE DI WATER since the system senses the resistance of the water to prevent running dry THE HUMIDIFICATION BOTTLE MUST BE EMPTIED WHEN NOT IN USE Cleaning The LiveCell 3 Controller Unit may only be wiped down with a damp cloth Avoid the use of solvents and abrasive cleansers The Chamber itself should never be cleaned with any ammonia based cleaners solvents or abrasive cleansers Use only cleaning products recommended for Acrylic Plexiglas Lexan and coated optical components DO NOT CLEAN THE CO2 SENSOR Service DO NOT ATTEMPT TO SERVICE THE EQUIPMENT unless otherwise instructed to do so by an authorized Neue Biosciences representative Doing so will void the warranty or service contract Fuses Replace only with same type and rating P N 05 07 0000 REV 02 Warnings page 1 Functional Description Secti
18. ssed by holding the SEL button down for 6 seconds or until the display looks like P N 05 17 0000 REV 02 Basic User Interface page 2 STEP 2 Press the SEL button once to transition into the adjustment menu then using the UP and DOWN buttons enter the desired adjustment STEP 3 Press the SEL button and hold for 3 seconds or until the Set Value is displayed The offset is now stored and will be used P N 05 17 0000 REV 02 Basic User Interface page 3 RS232 Interface special option p n 40302 Using HyperTerminal software located in programs accessories communications in a Windows based operating system the following instructions allow for the monitoring and setting of temperature and CO2 data Open HyperTerminal and start a New Connection from File pull down menu Choose following settings Com1 port serial port 9600 bits second 8 data bits None Parity and 2 stop bits Flow control to Hardware ANSIW format Confirm connection of RS232 cable from back of LiveCell to PC Serial port Type lt enter gt to view the version of the firmware LiveCell Version 2 There are six basic commands Type C lt enter gt to request the current set point and actual data for CO2 Type C 5 0 lt enter gt to change the set point to 5 0 CO2 can set up to 10 0 Type T lt enter gt to request the current set point and actual data for Temperature Type T 37 0 lt enter gt to change the set point to 37 0 deg C can set up t
19. ustment of the Alarm thresholds the point at which the Alarm LED illuminates e Initiation of the Self Tune process a function of the controller to maximize the stability of the control algorithm P N 05 17 0000 REV 02 General Capability page 1 All front panel operations of the controller can be accomplished through the optional RS232 interface as well Temperature Enable Switch The output of the temperature controller can be selectively enabled disabled by pushing the Temperature Enable Switch The green LED in the switch turns ON when enabled Temperature Alarm LED When the temperature Process Value is higher or lower than the Set Value by the Alarm Threshold then the Red Alarm LED will light as a visual indication that a problem exists No audible alarm exists Only when Set Value Alarm Threshold lt Temperature Process Value lt Set Value Alarm Threshold will the LED turn off Over Temperature Protection In the event of a misassembled system failure of one or more components or improper operation the unit has over temperature protection to keep the User from getting burned in the event of a thermal run away condition Humidity Control Inside Of The Microscope Chamber When the system is assembled properly it controls the humidity in the microscope chamber to the last entered Set Value The system uses a humidity sensor inside of the chamber to provide information the Humidity Controller requires for precise an
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