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Laboratory Safety Manual - Environmental Health and Safety

1. 3 15 2 Plastic and Glass iamen diea 3 15 3 Packaging Materials 3 15 4 Media and Printer 3 15 Batteries date m pde AE s 3 15 a One Time Battery Collection 3 15 b Routine Battery Collection 3 16 J SHARPS AND LAB GLASS 5 m n as gei Qu ta den ed do ede omuia Dea ot dera cad 3 16 DEEST 3 16 2 Lab Glass Broken 3 16 INFECTIOUS OR BIOLOGICAL WASTE 3 17 LE RADIOACTIVE WAS TE 3 doen ione a E Aer PUR hee encase RE EU CHR 3 17 M MXE P WAS TE tont picto Foo e bx HE Cobb Y 3 17 N LIQUID SCINTILLATION 3 17 ANIMALS AND ANIMAL BY PRODUCTS 3 17 1 Contaminated Animals and Animal By Products 3 17 2 Non Contaminated Animals and Animal By Products 3 18 Pe GAS CG VEIN DERS AAEE 3 18 Tables Table 3 1 Chemical Waste Toxicity Categories 3 4 Figures Figure 3 1 Hazardous Waste 3 7
2. 3 17 RADIOACTIVE WASTE enam neenon een edet nece per Mets 3 17 MIXED WASTE entere e eter ROBERT rettet retire Rr PR eerie herein iss 3 17 SCINTILIEATION COCK ATIS ti EHE re ert etti eet crete 3 17 ANIMALS AND ANIMAL BY PRODUCTS sess neret 3 17 1 Contaminated Animals and Animal By Products eese eene ene eene 3 17 2 Non Contaminated Animals and Animal By Products eee eese eene nennen enne 3 18 GASCYDBINDERS one erani cette ueber p Ree rb Per b AE e EG eU ERE EUER 3 18 SECTION 4 LABORATORY EQUIPMENT AND FACILITIES eere ee eene entente tne tn sensn 4 1 A EMERGENCY EYEWASHES AND SHOWERS esee nennen nennen retener 4 2 B FIRESAEFETY EQUIPMENT ERE EUR EHE CI Ur UE HESSE Ond 4 2 I Flammable Liquid Storage Cabinets ceive s iae e iate en eve pee eee ite e es 4 2 2 Flammable Storage Refrigerators eese eee nen nhen trenes rennen teer tnter nnne 4 3 C LABORATORY SIGNS 5 othoetr depone eni teen penat beasties 4 4 Le Emi ergency Numbers an kennnReAenantee en Rete p et e ERI 4 4 Qe Laboratory Floor Pl n aen esit pp On e A Soka Landaa Mei ONES 4 4 3 Emergency Safety Equipment Location Signs eese eese enne nennen enne nenne
3. ee tite t t ee Eee bee ile t eee e te been 4 15 4 Equipment Guards and Mounting eese eene trennen renes 4 15 92 Confiri d SPACES c etait epit teet eerte tt ee Ri d gio eee ene de PE 4 16 PRESSURE VESSELS AND 5 enne tenen enne nennen tren teen tren nennen nenne 4 16 ete EE P tee e ea E oe LER eerie 4 16 Z2 PressufexSystems t DR Rer n esp 4 16 3 SBreCOWIlOnsi EXORITUR RIS 4 16 G DECONTAMINATION OF WORK AREAS cessere 4 16 Il Custodial Services cuan ise e e ege ed ede tae ite de bote 4 17 2 Servicing of Lab Area or Equipment eese eene ener en rennen nete tenete trennen nenne 4 17 H DECONTAMINATION OF EQUIPMENT FOR DISPOSAL eee nennen rennes 4 17 1 Equipment Used to Process Store Chemicals essere nennen nennen rennen 4 18 2 Equipment Used to Process Store Radionuclides esee 4 18 3 Equipment Used to Process Store Biological Material eene 4 18 SECTION 5 EMPLOYEE HEALTH AND PPE eeeeeeeeee seen sesto sete suse ta sensn sens enses tuae 5 1 A ENVIRONMENTAL MONITORING AND MEDICAL SURVEILLANCE eene 5 2 Ll Exposure UMS Sr toda d e ETE Fe ERR 5 2 2x Special Chemical Air M
4. sse 2 19 Table 2 10 Organic Peroxide Forming Solvent 2 21 Table 2 11 Sensitizing or Allergenic Chemicals 2 22 Table 2 12 Flash Points and Flammability Limits of Some Chemicals 2 26 Table 2 13 Reportable if Shipped Chemical 2 28 Figures Figure 2 1 UW Hazard Label sse 2 13 Figure 2 2 Handwritten Label 2 13 Figure 2 8 HMIS 2 14 Figure 2 4 Peroxide Label UOW 1716 2 20 Figure 2 5 Not Ship Label 2 28 A BASIC LABORATORY SAFETY PRACTICES 1 Working Alone Do not work alone in the laboratory if the procedures being conducted involve highly hazardous substances or processes such as are described in section G later in this section If you are working alone with lesser hazard chemicals let personnel in other laboratories know of your presence or develop an accountability system with your supervisor or co workers 2 Prevent Chemical Exposure Prevent skin contact with chemicals For example use appropriate personal protective equipment PPE goggles gloves lab coat etc per Lab Safety Manual Section 5 B but consider it as the last line of defense and use other precautions such as using appropriate containment equipment and regularl
5. 2 3 3 Material Safety Data Sheets MSDSs esses eere nnee teen trennen trennen rennen ene 2 4 C CHEMICAL PROCUREMENT nee itte tee eth erret 2 4 l Hazardous Chemicals 3 eclipticae e Re tede 2 4 2 Pharmace uticals idi eee eU DRE HERR PERS UR UR P tueri 2 5 3 DEA Controlled Substance S reet m ette RE rete Rt rer HERE 2 5 4 Non D natured Ethyl Alcohol e ee eee IR do eR o Re bb ents 2 5 S Radioactive Materi ls ie sec be e deseri edere di led eee eed toe eoe ever ede dread EU 2 5 6 Highly Dangerous Materials s ee fr ea Ca e E ER PREX B eee 2 5 7 Compressed Gas Cylinder Procurement aeree eene ee eene rennen entente 2 6 6 Chemical EXCHANGE veal atc eR ono dae ase A e A A diee e eese 2 6 D CHEMICAL STORAGE Sele ae Re eS ee is Reis bee a 2 6 J Sepregate Incompatibles rrt ee P P REFUS TRUE 2 6 2 General Chemical Storage Guidelines eese eene nennen 2 7 3 Chemical Storage Quantity Limits ates tee e eo ee o eie SENSE EEA ee e pene ea EE er 2 8 UW Environmental Health and Safety Page ii Change 1 July 2010 Laboratory Safety Manual E CHEMICAL LABEBELING rete be EHE irte uae 2 12 I Original Container uisi bete eee eee ti HER ee e doe ego Pedes Pes etd diee 2 12 2 Tr nsfer
6. Limited shelf life Store tightly closed away from light and heat See UW Peroxide Guidelines or call 206 616 0595 for more information Peroxide Tester Peroxide Tester hitpJwww ehs washington edufforms epa peroxidequidelines pdf Testing Peroxides It is a good laboratory practice to use test strips to test the solvent for peroxides prior to each use After each use wipe down the bottleneck cap and threads with a cloth before resealing Reduce formed peroxides and add an inhibitor as necessary to keep the concentration of peroxides below 10 ppm Test and treatment methods can be obtained by calling EH amp S at 206 616 5835 Extreme caution should be exercised if concentrations of peroxides exceed 30 ppm Distillation and Evaporation Precautions Always test for peroxides before distillation or evaporation because these procedures will increase the concentration of any peroxides present Do not distill or evaporate solvents containing any amount of peroxides Use a water bath over a hermetically sealed electrical mantle to safely heat the solvent Use any distilled solvent immediately or add an inhibitor Use of Inhibitors Inhibitors slow the formation of peroxides in the future They do not reduce or remove peroxides Organic peroxides should be reduced safely Monitoring Expiration Date Use the solvent before the manufacturer s expiration date Peroxide forming solvents exceeding their expiration date cannot be
7. E 15 F LABORATORY MOVING OUT CHECKLIST enne nene enne innen tentent enne E 19 REQUIRED ELEMENTS CHECKLIST enne nnne nennen enn entente entere enne E 23 APPENDIX RESOURCES FOR LABORATORY PERSONNEL eese eee ense F 1 Av CALLING FOR ASSISTANGE e RE TU ERE Cre OM ipee F 1 B WEB RBSOURCGBS cece n F 8 APPENDIX G GLOVES Teo MD G 1 A gt CHOOSING GEOYV BS irent et i RA reme dba ty sus seus riri S G 2 Lo gBrm rysCOncem mea eager S nRSR USURIS np G 2 2 Glove Weaknesses ales ede eb ee ee evene eei beste eei eive G 2 B lt GLOVE MATERIALS e nc Pp ek via Cotas eh e tp ite eie en Eee G 2 1 Natra R bb r td e P ERR 2 2 xd tet et eee e etd uet G 3 EPI Hr E PP G 3 4 PVC unanime e i ER RS e Fes eee ERES E O agaadeonitvacatestacs G 3 NEM CUI 3 02 Rc C G 3 VEM DV CT El M G 3 Plastic Film ice ie eie eri eee eai ridet Pea a oid a eee ane Aa nde G 3 Gy JIEATEX AELBRGIC REACTIONS etr PROPERE ERE RP G 3 dc JrrHOOTWA Sis seit ea a aaa gas ee OI eI Pep ee aaNet Note tete tedio G 4 2 Delayed Hy
8. Also any one type of flammable chemical waste plus chemicals cannot exceed the limits specified by the controlling fire department For example in Seattle for class IA flammables which include ethers and other very flammable solvents the total volume of allowed flammables is limited to 60 gallons per control area in a sprinklered building and 30 gallons in a nonsprinklered building Contact EH amp S Building amp Fire Safety Office at 206 543 0465 with questions about control areas and volume limits if you accumulate large amounts of flammable hazardous waste or arrange for more frequent collection of this waste Leave some headspace at least one inch in each container to allow for pressure changes due to changes in temperature Chemical waste must not be accumulated i e stored for more than one year 6 Large Containers Drums If you are accumulating wastes in containers greater than five gallons in volume make sure that drums used to accumulate regulated wastes are in good condition and are approved by Department of Transportation DOT for highway mode transportation If the drums were shipped to you in the first place they are very likely DOT approved Drums containing liquids must have ten centimeters of air space between the liquid surface and the lid Collection must be requested before the drum is full especially in the case of 55 gallon drums 7 inherently Waste like Chemicals Inherently waste like chemicals
9. Fill out all information completely and fax or mail the form information is on the form 3 Routines and Routine Collection Requests Wastes that are generated on a regular basis may be set up as routine collections For routine collections EH amp S assigns your chemical waste a routine number To request pickup you then simply enter your routine number and waste volume in an online form EH amp S tracks what your waste is and where you are located If you have a routine waste number and want to request a pickup fill out and send the Routine Chemical Waste Collection Request at http Awww ehs washington edu forms epo routinepickup php To set up a new routine fill out and send a New Routine Chemical Waste Collection Request at http www ehs washington edu forms epo 1471 pdf If you have any questions about whether your waste is routine email chmwaste u washington edu or call 206 616 5835 4 Waste Cleanouts If you are moving or cleaning out your workplace and will need EH amp S to collect a large volume of chemical waste here are some guidelines If you think you have more than 100 containers of waste call 206 616 0595 to arrange a cleanout appointment Call at least a month before your deadline For fewer than 100 containers fill out and send the copies of the Chemical Waste Collection Request http Awww ehs washington edu forms epo 1470 pdf to EH amp S making sure to put your name on each of the pages Place compl
10. 1 30 6 Carcinogenic The Washington Department of Ecology briefly regulated chemical wastes that are suspected or known to be carcinogenic However these rules were challenged and the Department of UW Environmental Health and Safety 3 6 Change 1 July 2010 Section 3 Chemical Waste Management Laboratory Safety Manual Ecology retracted the rules EH amp S nevertheless strongly encourages you to manage chemical waste that is carcinogenic or mutagenic as if it were hazardous waste even if it is not toxic according to the definition above which accounts for acute and immediate toxicity 7T Trash Rules Non hazardous solid chemicals can go in the trash Known probable or suspected carcinogens irritants and sensitizers cannot go in the trash Deface labels Bag and label chemicals as non hazardous Liquids and pressurized containers like aerosol cans can never go in the trash Uncontaminated or slightly contaminated items such as gloves paper towels and empty containers can go in the trash EH amp S recommends double bagging and labeling these items as non hazardous if there is evidence of contamination Manage very contaminated items such as spill cleanup materials as hazardous waste Also empty containers for extremely toxic chemicals are hazardous waste unless you triple rinse them and dispose of the rinseate as hazardous waste 8 Local Sewer Limits These rules determine what can be poured down t
11. Figures Figure 2 1 Figure 2 2 Figure 2 3 Figure 2 4 Figure 2 5 Figure 3 1 Figure 4 1 Figure 4 2 Figure 4 3 Figure 6 1 Figure 6 2 Figure C 1 Figure C 2 Chemical Use Category Segregation Table 2 8 Example Hazardous Material Quantity Limits 2 10 Approved Flammable Liquid Storage Containers 2 11 Toxic Gas Generators sss 2 17 OXIDIZES fossa Se omes eee etus LAM ers 2 17 Chemicals that May Polymerize 2 17 Pyrophoric Chemicals esses 2 18 Water Reactive 2 18 Nitrated Compounds seen 2 19 Organic Peroxide Forming Solvent 2 21 Sensitizing or Allergenic Chemicals 2 22 Flash Points and Flammability Limits of Some Chemicals 2 26 Reportable if Shipped Chemical 2 28 Chemical Waste Toxicity Categories 3 4 Safety Related 510 4 4 NFPA Standard 704 Numeric Codes 4 6 Guidelines for Airborne Exposure Levels 5 2 Special Chemical Air 5 3 Hazards and PPE 2 5 etceter
12. The following are guidelines for the disposal of sharps and lab glass or broken glass that is not contaminated with infectious radioactive or chemical materials 1 Sharps Sharps are a restricted waste according to state and local regulators and must not be disposed of as special waste The term sharps is a regulatory waste classification associated with those instruments used to puncture cut or scrape body parts and that as waste can cause punctures or cuts to solid waste handlers or the public This is interpreted to mean that any instrument that looks like it is meant to be used in this manner must be disposed of as sharps waste The sharps definition includes but is not limited to hypodermic needles syringes IV tubing with needles attached lancets scalpel blades glass Pasteur pipettes microtome blades dental scalers and razor blades Such items must be disposed of in an authorized sharps container which is leak proof rigid puncture resistant and durable plastic It is red in color and equipped with a tight fitting lid for use during handling and transport Various sizes of sharps containers are available from different vendors Sharps containers should be labeled with the Principal Investigator s name and the room number and disposed of when full Sharps disposal like all biological waste at the University of Washington is dependent upon the location of generation Please refer to the location specific Biological Was
13. UW Environmental Health and Safety 3 5 Change 1 July 2010 Laboratory Safety Manual Section 3 Chemical Waste Management For mixtures such as diluted wastes and wastes containing more than one constituent an Equivalent Concentration EC for the mixture must be calculated to determine the toxicity level of the mixture If the EC is greater than or equal to 0 00196 the waste is toxic The formula for the EC is ZA a gt 4 2C xD 100 1 000 10 000 EC XX For example a mixture of 0 01 aldrin toxic category A 1 0 endrin toxic category A 4 0 benzene toxic category D 2 0 phenol toxic category C and 5 dinoseb toxic category B in water nontoxic exceeds the toxicity 1 0 0 01 5 096 2 0 4 0 0 153 10 100 1 000 10 000 EC 0 If you are not confident enough or willing to use the above equation to determine whether your chemical mixture is toxic please fill out and submit a Waste Evaluation Request online at http www ehs washington edu forms epo 1957 pdf EH amp S staff will then evaluate your waste and advise you on proper disposal of your chemical 5 Persistent Persistent chemicals do not biodegrade quickly in the environment There are two main categories of persistent chemicals described below a Halogenated Organic Compounds A halogenated organic compound HOC is a molecule that includes one or more atoms of fluorine chlorine bromine or i
14. corrections Now pages 5 6 e Preface Acknowledgements Office name change correction Now page 7 e Section 2 Changed wording Page 2 13 and corrected phone numbers from 206 685 5835 or 206 685 2848 to 206 616 5835 in several places Pages 2 19 2 20 e Section 3 Updated name of Chemical Waste Collection Request throughout and made other updates and typographical corrections such as phone numbers Added additional details to Section 3 D 5 What Happens to Hazardous Waste And Section 3 E 2 Trash Disposal of Empty Chemical Containers Pages 3 5 3 6 3 8 3 9 3 10 3 13 3 14 3 15 e Section 4 Corrected telephone numbers from 206 543 9510 to 206 543 0465 in several places Pages 4 8 4 9 4 18 e Appendix A Cover Page and Scope were updated to September 2010 to correct the content for formaldehyde and permissible exposure limit regulations Cover and Page 100 2 e Appendix B Corrected phone number to 206 616 5835 Page B 1 and added information about water concentration Page B 9 e Appendix E Checklist F Corrected telephone number to 206 543 0465 and deleted reference to University Stores Page E 19 and Checklist G SOP Required Elements Checklist Added additional explanatory material concerning particularly hazardous substances and revised the instructions section Page E 23 W UNIVERSITY of WASHINGTON m Interdepartmental Correspondence Environmental Health and Safety Director s Office Box 354400 206 543 7262 FAX 206 5
15. prepared or consumed in laboratories using chemicals Smoking is prohibited inside all University owned or occupied facilities and vehicles see http www ehs washington edu psosmoking index shtm a Glassware Utensils Glassware or utensils that have been used for laboratory operations must never be used to prepare or consume food or beverages b Storage of Food Beverages Laboratory refrigerators ice chests and cold rooms must not be used for food or beverage storage 5 Vacuum Use extra care when evacuating air from glassware Shield or wrap the glassware to contain chemicals and glass fragments should implosion occur When possible use thick wall vacuum glassware 6 Access to Emergency Exits and Equipment Storage even temporary storage and equipment must not block doorways corridors aisles stairways and laboratory emergency kickout panels to assure unobstructed access to exits in the event of an emergency Likewise emergency equipment such as eyewashes deluge showers fire extinguishers and fire alarm pull stations must be directly accessible 7 Laboratory Signs Laboratory signs must be posted as described in Section 4 C These signs may provide information e g emergency numbers prohibit unsafe behavior or require protective measures or designate locations of various supplies and equipment Magnetic or framed signs that can be easily moved may be used to designate a temporary hazard Warning signs must be
16. turn litmus paper red and have pH values of 0 to 6 An exposure limit designated in a WAC generally derived as an 8 hour time weighted average which requires the employer to initiate certain required activities such as exposure monitoring and medical surveillance An adverse effect on a human or animal body with severe symptoms developing rapidly and coming quickly to a crisis Also see chronic health effect The adverse acute effects resulting from a single dose of or exposure to a substance Ordinarily used to denote effects in experimental animals A dangerous material as identified with a dangerous waste number beginning waste with P in WAC 173 303 9903 Contact EH amp S at 206 616 5835 for current information alkali Any chemical substances which forms soluble soaps with fatty acids Alkalis are also referred to as bases They may cause severe burns to skin Alkalis turn litmus paper blue and pH values range from 8 to 14 alopecia Loss of hair analgesia Loss of sensitivity to pain anesthesia Loss of sensation or feeling anhydride An oxide or compound that when combined with water gives an acid or base anhydrous Free of water anorexia Loss of appetite anosmia Loss of the sense of smell anoxia A lack of oxygen from inspired air literally without oxygen Also see hypoxia UW Environmental Health and Safety 2 September 2009 Appendix B Glossary ANSI aqueous aquatic toxicity a
17. Chemical Hazards Added new sub section 10 Process Safety for Highly Hazardous Chemicals Previous editions of the Laboratory Safety Manual did not address this requirement Table of Contents and Page 2 29 e Section 3 B 7 Chemical Waste Management What Qualifies as Hazardous Waste Trash Rules Title Change Completely changed due to new rules from King County no longer using an approved trash disposal listing Waste chemicals potentially disposable into the trash must be checked for carcinogen sensitizer and irritant hazards in addition to previous hazardous waste definitions Table of Contents and Page 3 6 e Section 3 E 1 Trash Disposal of Chemicals Updated instructions in accordance with the changes in Section 3 B 7 Page 3 10 e Section 3 E 2 Trash Disposal Trash Disposal of Empty Chemical Containers added sentence referring to the EH amp S web page Page 3 11 e Section 3 F 3 Chemical Waste Management Sewer Disposal Chemical Treatment Log Title Change The Sewer Discharge Log is no longer required for disposal of chemicals However a Chemical Treatment Log is still required for those who treat chemicals to reduce their hazards and then discharge them to the sink Table of Contents Page 3 12 and Table 4 1 on Page 4 4 Minor Short Phrase or Single Word Changes e Preface Cover Page Identified as Change 1 July 2010 Page 1 e Preface October 7 2009 Letter from the Director Minor typographical and telephone number
18. If a container label becomes illegible during use you must affix an extra copy of the original container label or a completed generic label 2 Transfer to Additional Containers Chemicals are often transferred from the original container to another container This second container must be labeled with the chemical name which must be the same name as on the original container s label and the MSDS and hazard warnings Examples of the second container s label are shown below in Figures 2 1 and 2 2 The label should also include the initials of the person who made the transfer and the date of the transfer 3 Labeling Instructions Your department may require a specific type of label Describe any departmental or laboratory specific requirements for labels in the laboratory specific information section of your CHP Types of labels routinely used on containers are shown below The method of affixing the label to the container i e glue tape or wire is also at the discretion of the department laboratory Preparations sample aliquots etc do not need to be labeled if the container will be under the control of a person who knows what is in the container and it will be emptied before the end of the work shift If a preparation or working solution will be kept for a longer period the container must be labeled with the content identity and hazards and should be labeled with date of preparation and preparer s initials or name A container that i
19. July 2010 Section 3 Chemical Waste Management Laboratory Safety Manual 2 Outside King County If you are outside King County UW Tacoma Pack Forest and Friday Harbor local sewer limits have not been formally adopted in these areas In addition operators of some very small waste treatment plants allow chemical disposal to sanitary sewer only on a case by case basis in order to protect the treatment plant You are therefore not allowed to pour any chemicals down the drain without explicit permission at this time For more information and for assistance with obtaining permission to dispose of non hazardous chemicals to sanitary sewer call EH amp S at 206 685 3759 or email chmwaste u washington edu 3 Chemical Treatment Log All discharges must be recorded in a Chemical Treatment Log if you are treating waste Detergents bleach and other household cleaning chemicals are the only exceptions to this rule and do not need to be recorded Keep the log posted near the sink or point of discharge the emergency phone number on the Chemical Treatment Log form must be posted in the event of an accidental release of chemicals to the sewer Keep these logs for three years County inspectors can ask to see them EH amp S will also contact you annually for the total waste you treated each year UW is required to report waste treatment totals to state agencies See http Awww ehs washington edu epohazreduce index shtm for treating specific waste
20. access to MSDSs to surplus chemical exchange and to site specific chemical information including chemical inventories A particle having at least one dimension on the scale of 100 nanometers or smaller where chemical and physical properties may differ from bulk material properties Typically the term applies to deliberately human designed particles and not those which may occur in nature such as proteins or as a byproduct of other processes such as the release of nanoparticle sized combustion products Stupor or unconsciousness produced by some narcotic drug Tendency to vomit feeling of sickness at the stomach Local death of tissue A new or abnormal growth of tissue in which the growth is uncontrollable and progressive The environmental condition when the air pressure inside a room or containment device is less than the air pressure outside the area of interest When a fume hood is running it should be at negative pressure to the rest of the room This is desirable because hazardous chemicals inside the area of interest will be less likely to escape because air leaks will be into the area Also see positive pressure A method of chemically treating corrosive hazardous waste by the addition of an acid or base to make the waste neutral National Fire Protection Association An international voluntary membership organization to promote improve fire protection and prevention and establish safeguards against loss of life and prop
21. discarded through EH amp S until the contents have been tested for peroxides Examples of peroxide formers are shown in Table 2 10 below UW Environmental Health and Safety 2 29 Change 1 July 2010 Laboratory Safety Manual Section 2 Chemical Management Acetone cyanohydrins stabilized aluminum phosphide and phosphorus pentasulfide must be treated before collection Contact EH amp S for details before filling out the collection request if you wish to dispose of one of these three chemicals 10 Process Safety for Highly Hazardous Chemicals If there is any chance that the quantities of hazardous chemicals handled at one time may exceed the quantity limits of WAC 296 67 Process Safety Management of Highly Hazardous Chemicals additional safety precautions must be taken The basic regulation is viewable at http www Ini wa gov wisha rules hazardouschemicals default htm and a table listing chemical limits in pounds that require implementation of this process is at http www lIni wa gov wisha rules hazardouschemicals PDFs Chemchart pdf Among the requirements is a formal documented failure analysis using techniques such as e What lf e Checklist e Fault Tree Analysis e Hazard and Operability Study HAZOP e Failure Mode and Effects Analysis FMEA or e Other equivalent methodology for assessing hazards Assistance on these techniques is available from EH amp S 206 543 7388 Other requirements such as storing
22. handled on a case by case basis For more information on all these items see http Awww washington edu facilities transportation recyclingandsolidwaste 4 Media and Printer Cartridges Electronic media including CDs tapes cell phones and LaserJet cartridges and small amounts of household batteries are recycled in E MEDIA bins throughout campus This is a joint effort of UW Recycling and EH amp S Environmental Programs Office See the UW Recycling website for more information 5 Batteries Small amounts of batteries can be recycled through the E MEDiA system see above Large heavy and or unusual research or clinical batteries as well as large volumes of batteries are handled two ways a One Time Battery Collection To request a large one time collection of batteries fill out and send a Battery Collection Request at http www ehs washington edu forms epo 1943 pdf UW Environmental Health and Safety 3 16 September 2009 Section 3 Chemical Waste Management Laboratory Safety Manual b Routine Battery Collection Routine collection for batteries uses a process similar to that of hazardous chemical waste To set up a new routine fill out a New Routine Collection Request at http Awww ehs washington edu forms epo 1471 pdf If you have a routine number request a pickup with the online Routine Collection Request at http Awww ehs washington edu forms epo routinepickup php J SHARPS AND LAB GLASS
23. if this Chapter applies to your workplace use Table 1 Table 1 Chapter Application Are Hazardous Chemicals used Definition Hazardous chemicals are any chemicals that have been shown in at least one scientific study to cause acute or chronic health effects in exposed employees 296 839 WAC contains information that can be used to determine if a chemical is considered hazardous for this rule Are the hazardous chemicals used in laboratory scale operations Note Laboratory scale operations use containers that have been designed to be easily and safely handled by one person for reactions transfers and other handling of the hazardous chemicals If any one of your answers brought you here the Laboratory Standard does not apply but other WISHA rules still apply Laboratory scale operations are not Capable of producing commercial quantities of materials Part of a production process or simulate a production process Partof a quality control process that directs how a process operates simulation of a production process such as a pilot plant Are multiple chemicals or multiple procedures used Are protective practices or protective equipment generally available for employee protection Note Protective practices and equipment are those procedures practices or equipment accepted by laboratory health and safety experts as effective at controlling employee exposures to hazar
24. irritant CNS depressant Received Feb 10 2008 c HMIS Label HMIS is a commercially available rating and labeling system as shown in Figure 2 3 Developed by the National Paint and Coatings Association it is a complete hazard communication program in which the hazard ratings are related to personal protective equipment More information is available at http www paint org hmis index cfm These labels may be used for your containers The chemical s name and hazard ratings must be written on the label and the chemical s hazards must be described UW Environmental Health and Safety 2 14 September 2009 Section 2 Chemical Management Laboratory Safety Manual Figure 2 3 HMIS Label HEALTH FLAMMABILITY PHYSICAL HAZARD i PERSONAL PROTECTION d Globally Harmonized System GHS Labels The Globally Harmonized System for Classifying and Labeling Chemicals is a system coordinated internationally which uses standardized hazard terms warning statements and pictograms or icons on product labels The label format is also standardized If you are making an additional container label for a product labeled in accordance with the GHS you must include the product name and hazards but you do not need to include all the warning statements and pictograms or icons Information about the GHS labeling system is available at http Awww unece org trans danger publi ghs ghs rev01 O1files e html and http www os
25. lethal dose 50 A single dose of a material that on the basis of laboratory tests is expected to kill 50 of a group of test animals The LDs dose is usually expressed as milligrams or grams of material per kilogram of animal weight mg kg or g kg LEL or LFL Lower Explosive Limit or Lower Limit For a vapor or gas the lowest concentration lowest percentage of the substance in air that will produce a flash of fire when an ignition source heat arc or flame is present At concentrations lower than the LEL the mixture is too lean to burn Also see UEL or UFL lesion Abnormal change injury or damage to tissue or to an organ leukemia A progressive malignant disease of the blood forming organs LFL Lower Flammable Limit See LEL or LFL light sensitive Chemicals that may react violently or degrade in the presence of light Store in chemicals amber bottles in a cool dry dark place local exhaust A mechanical ventilation system for capturing and exhausting contaminants from the air at the point where the contaminants are produced welding grinding sanding other processes or operations as opposed to general exhaust The work area is often partially enclosed to improve the capture of the contaminants LSS Laboratory Safety System The name of the computer network database which has been upgraded and is now the MyChem system See MyChem malaise A feeling of general discomfort distress or uneasiness
26. m UW Environmental Health and Safety 24 September 2009 Appendix E Checklists Laboratory Safety Manual This is blank UW Environmental Health and Safety
27. of Elements 1 to 8 on Standard Operating Procedures SOP Form 6 6 Explanation of Elements on SOP Form for Particularly Hazardous Substances 6 7 Laboratory Specific C 1 Example Floor Plan see C 4 Page viii Change 1 July 2010 Laboratory Safety Manual Figure C 3 Example Chemical Safety Training Log C 6 Figure C 4 UW Laboratory Employee Safety Training Checklist C 7 Figure D 1 Blank Standard Operating Procedure SOP Form D 2 Figure 0 2 Example SOP for a D 4 Figure D 3 Example SOP for Acrylamide D 5 Figure D 4 Example SOP for Benzene Use D 6 Figure 0 5 Example SOP for Equipment Operation D 8 Figure D 6 Example SOP for Ethidium Bromide Use D 10 Figure D 7 Example SOP for Flammable Solvents Use D 12 Figure D 8 Example SOP for Formaldehyde Use D 13 Figure D 9 Example SOP for Gas Cylinder D 15 Figure D 10 Example SOP for Inorganic Acid D 16 Figure 0 11 Example SOP for Inorganic Base Use D 17 Figure 0 12 Example SOP f
28. recycling of materials that would otherwise be disposed of as hazardous waste See http Awww ehs washington edu eporecycle index shtm for all the common and sometimes uncommon items recycled at the UW from batteries to computer monitors to elemental mercury to scrap metal l SOLID WASTE AND RECYCLING Below are guidelines for recycling a number of common non chemical items in laboratories 1 Paper and Cardboard EH amp S encourages you to recycle boxes and packaging as soon as possible unless you have sufficient storage space for them Storing boxes in aisles or in front of emergency equipment or exits or necessary fire panels is illegal and dangerous Paper cardboard and other common recyclables are managed by UW Recycling For more information see UW Recycling s procedures webpage at http Awww washington edu facilities transportation recyclingandsolidwaste 2 Plastic and Glass Plastic and glass chemical containers are not recyclable at this time The glass and plastic recycling industry uses recycled material to make food and beverage containers and bans chemical containers even if rinsed clean from their recycling streams UW Recycling and the EH amp S Environmental Programs Office are currently pursuing limited recycling for some laboratory plastics 3 Packaging Materials UW Recycling also coordinates the recycling of wooden pallets packaging peanuts plastic wrap and other packaging materials Styrofoam packaging is
29. removed when the hazard no longer exists such as a sign indicating the presence of a chemical that is no longer kept in a laboratory UW Environmental Health and Safety 2 5 Change 1 July 2010 Laboratory Safety Manual Section 2 Chemical Management B CHEMICAL INVENTORY AND MATERIAL SAFETY DATA SHEETS Laboratories must maintain chemical inventories in MyChem the University of Washington s campus wide chemical tracking system MyChem is designed for emergency planning efforts and helps laboratories comply with federal state and local regulations Chemical inventories location contacts and Material Safety Data Sheets MSDSs are provided to emergency personnel so they know what chemicals may be involved in an accident and who to contact in the event of an emergency Laboratories use chemical inventories to keep track of chemicals and to avoid unnecessary purchases Laboratory staff are required to maintain their chemical inventories in MyChem to facilitate compliance with Bothell Seattle or Tacoma Fire Department Hazardous Material Storage and Use Permits occupancy permits EPA Community Right To Know reporting and Department of Homeland Security chemical security requirements MyChem also stores more than 425 000 MSDSs MSDSs provide an overview of the hazards of products used in the laboratories All employees should be able to readily access an MSDS for any chemical they are using 1 Access to MyChem For access
30. safe area e g not a windowsill Waste chemicals may be stored with unused chemicals as long as the containers are properly labeled and your laboratory personnel know the storage location Do not accumulate large amounts of waste in the fume hood Use flammable liquid storage cabinets for flammable waste over ten gallons in volume Store the waste away from emergency equipment such as safety showers and emergency access panels Do not block exits Do not store the waste near or in sinks If the waste is stored in an area that drains to a floor drain the waste must be in secondary containment UW Environmental Health and Safety 3 8 Change 1 July 2010 Section 3 Chemical Waste Management Laboratory Safety Manual 4 Segregation Segregate regulated chemical waste by chemical compatibility Refer to the segregation guidelines in Section 2 of this manual Use secondary containment tubs basins or buckets for segregation of incompatible wastes accumulated in the same area 5 Accumulation Volume Limits Accumulate no more than 200 liters 55 gallons of chemical waste per waste stream or one liter one quart of extremely hazardous waste per waste stream Extremely hazardous waste is waste that is highly toxic and the one liter limit is designed to limit risk especially in the event of a spill See http www ehs washington edu epowaste ehw shtm for how to determine whether your waste is extremely hazardous waste
31. that are explosive when dehydrated Purchase nitrated compounds in small quantities Do not break the seal on the cap until the chemical is needed When you purchase a nitrated compound weigh the container and note the weight on the bottle Prior to subsequent use weigh the container again If the container weighs less add an appropriate solvent to replace the weight lost After the reagent is opened and an aliquot is taken again note the weight of the container Visually inspect the container for problems prior to each use and wipe down the bottleneck cap and threads with a wet cloth before resealing Additional factors need to be addressed in your SOPs are described in the opening paragraph of Section G above Examples of nitrated compounds are shown in Table 2 9 Table 2 9 Nitrated Compounds Diphenyl hydrazine Nitrocellulose 3 Nitrotoluene Trinitrobenzene Trinitrophenol Picric acid Trinitrotoluene Picric acid is a nitrated compound usually purchased as a solid wet with 1096 water Extreme heat blasting cap or electric charge can detonate picric acid It becomes highly unstable if allowed to dehydrate When wet picric acid is an orange colored compact crystalline solid with the consistency of lumpy sand When dry picric acid is a crystalline solid with visible air pockets below the surface Picric acid will readily form explosive metal picrates These metal picrates are extremely shock se
32. to MSDSs go to http mychem ehs washington edu and login using your UWNetID and password To request access to site specific chemical inventories and the Chemical Exchange register your name and your specific inventory locations at https www ehs washington edu pubcookie epo mychemcomboform php or send an email to mychem uw edu MyChem is easy to use but if you have questions or desire additional information a MyChem User s Manual is available on the MyChem web site http mychem ehs washington edu You may also phone EH amp S at 206 616 4046 Hands on computer training is available for groups upon request Register at http Awww ehs washington edu psotrain corsdesc shtm mychem 2 Conducting your Chemical Inventory Personnel must inventory all chemicals found in the laboratory and specify the maximum amount normally found at this location Dilutions and reagents prepared in the lab for further work do not need to be added to the inventory but must have a container label applied unless the preparation will be all used or disposed that day Review and update inventories annually and whenever there are significant changes in your chemical inventory such as when you are moving a laboratory or starting a new project A worksheet that helps you inventory prior to entry into MyChem is available at http www ehs washington edu epomychem mychemworksheet pdf While conducting your inventory examine containers for deterioration and inte
33. 206 221 7770 to update your Research Project Hazard Assessment RPHA Form and or laboratory spaces a If you are relocating or ending research involving select agents contact the EH amp S RBSO at 206 221 7770 for instructions a If you intend to relocate a biological safety cabinet call 206 543 0465 or complete and submit a Request to Purchase or Relocate a Biological Safety Cabinet at http Awww ehs washington edu fsobiocab approvedlist2 shtm a If applicable submit written plans for the decommissioning of a Biosafety Level 3 BSL 3 area to the EH amp S Research amp Biological Safety Office Manager Box 357165 For questions or assistance call EH amp S Hesearch amp Biological Safety Office at 206 221 7770 Radiation Safety Notify the EH amp S Radiation Safety Office in writing as soon as the intent to vacate is known Mail correspondence to EH amp S Radiation Safety Box 354400 or e mail radsaf u washington edu Inform Radiation Safety of your new laboratory location if known Discuss arrangements with Radiation Safety to assure removal of all radioactive waste and to coordinate relocation or transfer of ownership for remaining radioactive materials 206 543 0463 UW Environmental Health and Safety 20 September 2009 Appendix E Checklists Laboratory Safety Manual If the Principal Investigator is leaving the University of Washington these additional steps must be followed with the Radiation Safety O
34. 43 3351 www ehs washington edu October 7 2009 TO Principal Investigators and Laboratory Supervisors FROM Jude Van Buren Dr PH MPH RN ub Director SUBJECT UPDATED LABORATORY SAFETY MANUAL am delighted to provide for you this revision of the University of Washington s Laboratory Safety Manual This manual was developed in a collaborative effort between the members of the Chemical Hazards Advisory Committee CHAC the Environmental Health and Safety Department and UW researchers would like to thank all who contributed to this revision and in particular want to acknowledge and thank the CHAC chair Elaine Faustman Professor Environmental and Occupational Health Sciences and the following CHAC members Paul Hopkins Professor and Chair Chemistry Gary Pedersen Director Chemistry Lia Wetzstein Environmental Science Lab Coordinator and Lecturer Interdisciplinary Arts and Sciences Tacoma Campus Tony Miller Buyer Purchasing Sam Tillery Manager Facilities Services Health Science Maintenance Zone Major efforts have been made to make this revision more user friendly and believe that you will find it current addressing the latest worker safety and environmental regulations as well as more concise and easier to understand You are encouraged to frequently review this manual with your employees to assure they understand the key University policies and local state and federal regulatory requirements under whic
35. IST Instructions Use this SOP checklist to ensure that all required safety elements are present in an existing SOP There may be an instance where a required element is not applicable under certain specific circumstances such as when no animals are involved Mark those elements with an NA Add any missing elements to your existing SOP and add the completed checklist to your laboratory specific information section in your Chemical Hygiene Plan SOP Title Date PI or Lab Name Department Element General Required Elements Present Process or Experiment Description Are the chemicals process or equipment identified well enough there will be no confusion as to what the SOP pertains to and doesn t pertain to Hazardous Chemicals Class of Hazardous Chemicals Are all hazardous chemicals chemical classes involved in the procedure addressed raw Stocks intermediates final products and wastes PPE Personal Protective Equipment Is the personal protective equipment to be used described well enough that there will be no confusion as to what is required at what stages of the procedure Engineering Ventilation Controls Are the environmental controls and ventilation systems needed to safely use the chemicals identified This may include hoods environmental rooms aerosol suppression devices filtering or absorption devices etc Does the SOP note that the equipment must be checked for proper operation be
36. Limits 3 11 2 Outside King County eene 3 12 UW Environmental Health and Safety 3 2 Change 1 July 2010 Section 3 Chemical Waste Management Laboratory Safety Manual 3 Chemical Treatment Oficio 3 12 4 Soaps Bleach and Acetone 3 12 5 Scintillation Fluids sse nnn 3 12 6 Dil tion Prohibitlon i etcetera 3 12 CHEMICAL WASTES OF PARTICULAR 3 13 1 Unknown Chemicals sss 3 13 2 Potentially Explosive 3 13 a Peroxide Forming 3 13 b Picric Acid and Other Polynitroaromatic Compounds 3 13 c Sodium Azide ede teret ee ete 3 14 d amp diraa 3 14 3 Legacy Chemicals 3 14 HAZARDOUS WASTE MINIMIZATION o s 3 14 1 Chemical Procurement and Chemical Exchange 3 14 2 Treatment and Recycling in the Laboratory 3 14 3 Hazardous Materials 3 15 I SOLID WASTE AND 3 15 1 Paper and
37. Mandatory Section 4 C 3 Food and drink prohibitions Mandatory if present Section 4 C 4 Area and equipment warnings Mandatory if present Section 4 C 5 NFPA 704 See Section 4 C 6 Section 4 C 6 Chemical Treatment Log for waste disposal sink Mandatory if present Section 3 F 3 Natural gas emergency shut off valve Mandatory if present Laboratory water do not drink Mandatory if present Lab specific procedural operational signs Optional Desirable Section 4 C 7 1 Emergency Numbers Post a list of telephone numbers to be called in case of fire accident hazardous chemical spill or other emergency The list should be posted prominently in each laboratory next to a telephone 2 Laboratory Floor Plan A plan showing evacuation route s as well as emergency and safety equipment locations should be posted prominently in each laboratory See Appendix C for an example laboratory floor plan If particularly hazardous substances are used in a designated area the floor plan is mandatory 3 Emergency Safety Equipment Location Signs Signs must be posted identifying the location of exits safety showers eyewash stations fire extinguishers first aid equipment flammable storage cabinets and other safety equipment Contact Facilities Services to post these signs 4 Food and Drink Prohibitions Label areas refrigerators freezers and other locations where food and beverages are not to be consumed or stored Food prohibitio
38. NP RUE RR REM ee 6 4 32 Step 3 Complete the SORS EUR m E NRI bn 6 5 4d jStep4 FietheSOPsu cedendo idem eee ote Beas ea kt 6 5 5 Distributing Copies of the SOPS 6 5 6 Update SOPs as Needed ee e er PR HERE Hr EUH ERU Eee egi 6 5 SECTION 7 SAFETY TRAINING reiris iiciin oerein Rei near rinia n statu stas tatus TEREE 7 1 TRAINING eene nennen netten nennen nette then tren tnit nenne ene ener n nenne 7 2 I Employee Safety Lraining s tr RE He Fn EH EEUU Petro diee Pen peer 7 2 2 BEES Safety Training ou ae nig hs ae os EE nee qne pa Eda d ae 7 2 3 Employee Safety Training baee ver perde ie E pei EE eE 7 2 B LABORATORY SPECIFIC SAFETY TRAINING CONTENTS sess een eene 7 2 1 Laboratory Safety Manual Chemical Hygiene Plan eese eee nennen enne enne 7 2 2 Permissible Exposure Limits 4 et RR etit eter te e ch onan ta eei 7 3 3 Material Safety Data Sheets i eid eec n tenen aer e D a eO 7 3 4 Workplace Hazards ud t eed ee decipi eset aet dede ce iyu ees Ip eee eta tae 7 3 2 Personal Protective Equipment gei re a e ce eee e ape bti 7 4 6 Chemical Storage arid Labeling wi ee EE ne UR cate 7 4 Laboratory Safety Manual Change 1 July 2010 7 Laboratory Was
39. Only allowed in sprinklered buildings Water Reactives Class 1 5 pounds or 2703 1 1 Limit depends on class to 3 Highly Toxic Materials 10 pounds or 2703 1 1 Gas may only be used and stored in UW Environmental Health and Safety 2 11 Change 1 July 2010 Laboratory Safety Manual Section 2 Chemical Management Material Quantity Limits IFC Comments per Control Zone Citation 20 cubic feet gas approved ventilated cabinets or exhausted enclosures Corrosives 810 cubic feet 2703 1 1 Limits increased if stored in approved cabinets or the zone is sprinklered Liquid Corrosives 500 gallons 2703 1 1 Same as Corrosives Toxics 500 pounds 2703 1 1 Same as Corrosives b Flammable Liquids in Basements In Seattle flammable liquids are prohibited in basement laboratories except for laboratories conforming to Seattle Fire Department Administrative Rule 34 03 04 The rule is online at http Awww seattle gov fire FMO firecode adrules AdRule34 03 04 pdf For assistance in determining local requirements contact EH amp S at 206 543 0465 c Additional Requirements In a laboratory a maximum of 10 gallons of flammable liquids in approved containers may be stored outside of a flammable liquid cabinet See the following table Table 2 3 Approved Flammable Liquid Storage Containers for container types and limits Reference NFPA 30 Table 9 4 3 Flammable liquid c
40. Page vi Change 1 July 2010 Laboratory Safety Manual APPENDIX A WAC 296 828 HAZARDOUS CHEMICALS IN A 1 APPENDIX 5 B 1 APPENDIX C TEMPLATES FOR LAB SPECIFIC INFORMATION eeeeee seen ee ense tn seta seta seta sea sonata C 1 A LABORATORY SPECIFIC INFORMATION essere nennen enne entren nnne tenen C 1 B LABORATORY FLOORPLANS eine ertet ie ure vie bieten pter e pex iet e eb eee REUS C 4 C TRAINING DOCUMENTATION FORMSS esesseseeeeeeeeeeee nennen enne tenen nest tenen C 5 APPENDIX D EXAMPLE STANDARD OPERATING D 1 A BLANK STANDARD OPERATING PROCEDURE SOP FORM creen enne D 1 B EXAMPLE STANDARD OPERATING PROCEDURE SOP 5 D 3 APPENDIX CHECKLISTS A M E 1 A EARTHQUAKE PREPARATION CHECKLIST FOR LABORATORY E 3 B ANNUAL LABORATORY SAFETY SURVEY CHECKLIST eese enne nennen E 5 C TOP 25 LABORATORY SELF AUDITING CHECKLIST INSTRUCTIONS eee E 8 D TOP 25 LABORATORY SELF AUDIT CHECKLIST eese eene enne enne enne E 9 E MOVING IN NEW LABORATORY CHECKLIST esee enne
41. Several nitrocellulose products primarily paper and tubes are used in some laboratories Nitrocellulose burns vigorously in ambient conditions and may explode when heated under confinement When completely dehydrated it is considered a low level explosive As a result these products should never be autoclaved for decontamination Nitrocellulose products must be soaked in water before disposal through EH amp S 3 Legacy Chemicals Principal investigators are required to completely clean out laboratories before they leave including all hazardous chemicals and waste see Section 10 Moving In Moving Out However sometimes people leave without disposing of chemicals properly Legacy chemicals are unwanted chemicals that are sometimes left behind after a move If you move into a laboratory that has legacy chemicals in it you should tell your department administrator immediately If your department cannot for whatever reason solve the problem then these legacy chemicals are yours to manage Unless you think that you will use them arrange to request their collection as hazardous waste and follow all waste accumulation rules including hazard identification labeling and segregation H HAZARDOUS WASTE MINIMIZATION On average EH amp S collects and processes about 200 000 kg of hazardous chemical waste a year Since 1985 EH amp S has developed several programs to reduce the amount of hazardous waste that must be incinerated or landfil
42. TE ACCUMULATION RULES 3 6 1 Appropriate 3 6 2 Hazardous Waste Labels 3 6 Be Locat Mae trt t adiri enero 3 7 4 Segregalloli doen ap d a Fu ibit 3 8 5 Accumulation Volume Limits 3 8 6 Large Containers Drums sse 3 8 7 nherently Waste like 1 2 2 3 8 D HAZARDOUS WASTE COLLECTION 3 8 1 Hazardous Waste Collection 3 8 2 Collection Requests One Time sssseeeeeeee 3 9 3 Routines and Routine Collection 3 9 4 Waste Cleanouts sess entree tnn 3 9 5 What Happens to Hazardous Waste 3 9 E IHASH DISPOSAL s fate lacs ies ec pect ewes ca hed Ate eee 3 10 1 Trash Disposal of Chemicals cccccceceeseeseeeeeeeteeeeeaeeeeeeeesees 3 10 2 Trash Disposal of Empty Chemical Containers 3 10 3 Trash Disposal of Contaminated Items 3 11 F SEWER DISPOSAL iste ome terere buen Sic ges Se corda coheed aedes coe 3 11 1 King County Local Sewer Discharge
43. W UNIVERSITY of WASHINGTON Laboratory Safety Manual September 2009 Including Change 1 July 2010 I Environmental Health and Safety 444295 UNIVERSITY of WASHINGTON Environmental Health and Safety Department University of Washington Box 354400 Seattle WA 98195 4400 Phone 206 543 7262 FAX 206 543 3351 www ehs washington edu Change 1 July 2010 to the September 2009 UW Laboratory Safety Manual Identified with columnar lines at locations changed Major Paragraph Page Changes Corrections and Additions e Preface Inserted Change Record as page 3 with blank page 4 other preface pages moved back e Section 2 B Chemical Inventory and Material Safety Data Sheets and 2 B 1 Access to Mychem Reworded to improve clarity and internet links Page 2 5 e Section 2 C Chemical Management Chemical Procurement Completely updated paragraphs 2 Pharmaceuticals and 3 DEA Controlled Substances and reworded paragraphs 4 and 7 to fit revised paragraphs 2 and 3 on the page Page 2 7 e Section 2 D 3 Chemical Management Chemical Storage Chemical Storage Quantity Limits Updated information about control zone limits decreasing on upper floors and corrected the table showing allowable container sizes and its reference such that metal containers could be used for quantities greater than 5 liters for some flammable and combustible liquids Pages 2 10 and 2 11 including Tables 2 2 and 2 3 e Section 2 G Chemical Management Special
44. a tenter 5 4 General Purpose Chemical Spill Kit Contents 9 8 Mercury Spill Kit Contents ssseeee 9 9 Typical First Aid Kit Contents ssssseeeees 9 9 Classes of Fires and Proper Fire Extinguishers 9 10 Radionuclides in Use and Their ALI Groupings 9 16 Evaporation Rate B 6 Glove Size S ia ia n net betreut Ine eerte dede repere G 5 Glove Suppliers sesssssseseeeeeeeeeennnne enne G 5 Glove Guide for Specific G 6 Minimum List of Known Particularly Hazardous Substances H 4 Particularly Hazardous Substances Listed by CAS Number H 18 UW Hazard 2 13 Handwritten Label 2 13 HMIS Label t fette eta Pho ted 2 14 Peroxide Label UOW 1716 2 20 Do Not Ship Label sse 2 28 Hazardous Waste 3 7 Biohazard Warning Symbol sse 4 5 Radiation Warning Symbol sss 4 5 NFPA Standard 704 Hazardous Material Sign 4 5 C PARTICULARLY HAZARDOUS SUBSTANCE LIST Page vii Change 1 July 2010 Explanation
45. abilization 2 Explosive Capability Peroxides formed in organic solvents have caused some laboratory accidents including unexpected explosions during distillation and use UW Environmental Health and Safety 2 20 Change 1 July 2010 Section 2 Chemical Management Laboratory Safety Manual Such formulations are considered low powered explosives in that they will detonate in moderate concentrations by modest shock friction or when heated The biggest dangers of organic peroxides in these solutions are opening the container and distilling Do NOT open or move the container if you see crystals on or around the container cap Call for assistance if you are concerned about opening the container EH amp S 206 616 5835 Required Procedures Purchase peroxide forming solvents in small quantities that contain an inhibitor such as butylated hydroxytoluene BHT which will delay the formation of peroxides until the inhibitor is used up Label the container with the date received and opened Label the container with the standard peroxide label UoW 1716 see Figure 2 4 below Do not break the seal on the container until the solvent is needed Once opened store solvent in an airtight amber glass bottle or metal container with an inert gas such as nitrogen in the headspace Figure 2 4 Peroxide Label UoW 1716 WOW 1716 10 07 INHIBITOR Date Received L d Yes No Date Opened Date Expires Type
46. an out of sorts feeling mechanical exhaust A powered device such as a motor driven fan or air stream venturi tube for exhausting contaminants from a workplace vessel or enclosure medical consultation Consultation which takes place between an employee and a licensed physician or other healthcare provider for the purpose of determining what medical examinations or procedures if any are appropriate in cases where a significant exposure to a hazardous chemical may have taken place melting point The temperature at which a solid substance changes to a liquid state For mixtures the melting range may be given UW Environmental Health and Safety 10 September 2009 Appendix B Glossary mil MSDS mutagen MyChem nanoparticle narcosis nausea necrosis neoplasm negative pressure neutralization NFPA NIOSH NTP nystagmus olfactory Laboratory Safety Manual Generally one one thousandth of something With respect to protective gloves a unit of thickness equal to one thousandth of an inch Thin surgical gloves may be five to seven mils thick Many industrial gloves are 20 to 35 mils thick Material Safety Data Sheet A document describing a chemical s known hazards which is produced by the chemical manufacturer and provided to the chemical user as required by OSHA A substance or agent capable of altering the genetic material in a living cell A computer network database established to give
47. ate chemicals in the hood to dispose of them Any open apparatus used in hoods which emit large volumes of volatile chemicals should be fitted with condensers traps or scrubbers to contain and collect hazardous vapors or dusts Storage Do not store chemicals or supplies in the fume hood Chemicals and supplies should be stored in approved cabinets Flammable Liquid Vapor Laboratory fume hoods are designed to reduce flammable vapors below lower explosive limits when properly operated and maintained As an added precaution use only non sparking and explosion proof electrical equipment hot plates stirring plates and centrifuges in fume hoods where a large volume of flammable liquid vapor may be generated Take care with flammable liquids and heat sources Containers All containers of chemicals must be securely capped when not in use A rule of thumb is that containers should be open for minutes at the most which is the maximum time it normally takes to pour a small amount of chemical into another container and cap them All containers must be labeled with the chemical identity and appropriate hazard warnings or the material must be used up during the work period and it is under continuous control of the researcher using it b Fume Hood Prep for Maintenance 1 2 3 Prior to any maintenance of fume hoods the entire interior surfaces must be decontaminated and or cleaned as described below in Section G 2 Decontamin
48. ation of Equipment for Service by the researchers using the hood Maintenance may require access to the storage cabinets below the hood or to the sides of the hood If this access is required the entire cabinet and adjacent area also needs to be emptied decontaminated cleaned and rinsed Lab staff need to identify a contact for coordinating with Facilities Services as to the work to be done See Section G 2 below for details and the required form c Fume Hood Testing 1 EH amp S performs a functional performance test annually to assure hoods are performing as designed If a hood fails it may need to be taken out of service until repaired EH amp S will notify the researchers and post a do not use sign if repair is required If you are having problems with your fume hood contact EH amp S at 206 543 0465 EH amp S will troubleshoot the problem and may refer it to Facilities Services for repair 3 Perchloric Fume Hoods Procedures using concentrated perchloric acid 27096 or which heat any amount or concentration of perchloric acid must be performed in a closed system or within a specially designed perchloric acid fume hood with wash down systems to prevent the accumulation of explosive perchlorates in the hood and ducting For assistance in locating a perchloric acid fume hood call EH amp S at 206 543 0465 UW Environmental Health and Safety 4 9 Change 1 July 2010 Laboratory Safety Manual Section 4 Laborat
49. bout the chemical by examining the container and interviewing anyone you think might know something about the chemical If that fails complete and mail or fax to EH amp S a Chemical Waste Collection Request online at http www ehs washington edu forms epo 1470 pdf Provide as much information about the waste as possible such as the history physical properties and the results of any analysis performed on the unknown Identification analysis performed by the approved waste disposal contractor will cost the chemical user roughly 80 per unknown Analysis performed by the contractor is conducted in the area where the unknown is stored After analysis EH amp S can collect the unknown for hazardous waste disposal 2 Potentially Explosive Wastes Some common chemicals can become highly unstable explosives over time when stored improperly and cannot be collected as hazardous waste unless they have been deactivated and stabilized The following segments highlight the most common of these troublesome chemicals a Peroxide Forming Chemicals Peroxide forming chemicals such as p dioxane diethyl ether tetrahydrofuran and acetaldehyde that have exceeded the manufacturer s expiration date will not be collected for disposal until they have been tested for peroxides These chemicals must be managed correctly For more information see section 2 G 2 b earlier in this manual and the EH amp S Peroxide Forming Chemicals Management and Assessment Guidelines on
50. cals or working around the chemicals must be able to demonstrate that they can retrieve the MSDS for a chemical within a short period such as within five minutes MyChem allows researchers to link to electronic and updated MSDSs directly so is a suitable tool for fulfilling this requirement EH amp S recommends laboratories maintain paper copies of MSDSs for the hazardous chemicals most likely to spill and or cause injury to someone Having an MSDS immediately available when someone has been exposed to a hazardous chemical helps emergency personnel decide how to respond and treat that person Call EH amp S at 206 616 3441 to request assistance locating or accessing MSDSs during business hours For MSDSs which are in the MyChem system EH amp S will obtain the MSDS for you and fax a copy within a work shift Chemicals that do not have an MSDS in the system will take longer to research and obtain After business hours contact the UWPD at 206 685 8973 UWPD will contact an EH amp S representative A Safety Data Sheet SDS generated in accordance with the Globally Harmonized System of Classification and Labeling of Chemicals provides similar information to an MSDS and is an acceptable alternative to an MSDS If an MSDS or SDS is received with a chemical shipment please maintain a copy in the lab and send the original MSDS or SDS to EH amp S Box 354110 c o MSDS Coordinator for addition to the MyChem database C CHEMICAL PROCUREMENT Most ch
51. dor must be used to provide a specialty gas the purchaser must get a written return agreement from the distributor or manufacturer prior to purchasing the gas It is UW Environmental Health and Safety 2 8 September 2009 Section 2 Chemical Management Laboratory Safety Manual important that the return agreement include a statement requiring the manufacturer to take back both the cylinder and any unused gas The purchaser should retain this agreement until the manufacturer has accepted the returned cylinder 8 Chemical Exchange The UW Chemical Exchange program facilitates the free exchange of chemicals campus wide via MyChem the online chemical inventory system Consider checking the online Chemical Exchange accessible only to UW employees for chemicals before you buy new chemicals For more information about this program see the MyChem website at http Awww ehs washington edu epomychem index shtm D CHEMICAL STORAGE 1 Segregate Incompatibles To avoid dangerous interactions among incompatible chemicals chemicals should be physically segregated by observing the general classes listed in Table 2 1 and by checking the MSDS Incompatible chemicals within these classes should also be segregated You can contact EH amp S at 206 543 7388 for additional information about chemical hazard classes and compatible storage Table 2 1 Chemical Use Category Segregation Table Segregate acids from active metals such as sodium pota
52. dous chemicals For example laboratory fume hoods chemical splash goggles protective gloves etc or Those practices procedures or equipment the employer can show are effective at controlling employee exposures to hazardous chemicals If your answers brought you here the Laboratory Standard applies to your workplace Continued http www LNl wa gov 100 1 04 06 Hazardous Chemicals in Laboratories VVAC 296 828 100 Scope WAC 296 828 100 Scope Continued IMPORTANT When your laboratory operation is covered by this Chapter and you use any of the substances in Table 2 the following applies with the exception of formaldehyde use in histology pathology and anatomy laboratories In histology pathology and anatomy laboratories you must follow the requirements in chapter 296 856 WAC Formaldehyde This chapter applies to all other formaldehyde laboratory uses as defined in Table 1 The exposure limits and any requirement protecting employees from skin and eye contact in the rules listed in Table 2 will still apply Where the action level or where no action level exists the permissible exposure limit is exceeded for a substance listed in Table 2 the exposure evaluation and medical surveillance requirements in the substance rule will still apply You aren t required to meet other requirements of the substance rule Togetthe permissible exposure limits PELs for hazardous c
53. ds for Chemical Abstracts Service a Columbus Ohio organization that indexes information published in Chemical Abstracts by the American Chemical Society and provides UW Environmental Health and Safety 9 Change 1 July 2010 Laboratory Safety Manual Appendix B Glossary jaundice Yellowish discoloration of the skin whites of eyes and bodily fluids with bile pigment bilirubin caused by any of several pathological conditions that interrupt liver function L amp l Department of Labor and Industries The State of Washington agency that is responsible for administering worker safety and health regulations in Washington www wa gov ini laboratory An area where chemical manipulations are done for either research educational or clinical purposes Laboratory Safety The precursor to MyChem No longer in use See MyChem System lacrimation Secretion and discharge of tears lavage A washing of a hollow organ such as the stomach LC lethal The concentration of a material that on the basis of laboratory tests has been concentration 50 shown to kill 50 of a group of test animals when administered as a single exposure usually 1 or 4 hours The LCs is expressed as parts of material per million parts of air by volume ppm for gases and vapors or as micrograms per liter of air ug l or milligrams per cubic meter of air mg m for dusts mists gases or vapors or as ppm or mg l by mass of material in water 05
54. e hood designs which have been approved for purchase on the EH amp S web site at http Awww ehs washington edu fsofumehoods approvedfumehoods shtm Additional information about fume hoods and access to an on line training class in fume hood operation is available at http www ehs washington edu fsofumehoods index shtm a Fume Hood Use 1 Training Personnel using fume hoods should take the on line training class at http Awww ehs washington edu fsofumehoods index shtm or equivalent 2 Verify Operation Make sure the fume hood is operating before starting work Some new fume hoods have monitoring devices that indicate acceptable working conditions Otherwise a strip of Kimwipe taped to the underside of the sash can be used as an indicator of air flow Since this strip may flutter even when the air flow is inadequate the strip should be placed and its movement observed when you know that the air flow is proper such as at the same time that EH amp S measures the air velocity 3 Exhaust Fan Speed Laboratory fume hoods in recently remodeled and newer buildings have two speed exhaust fans with local control at the hood The low exhaust setting is only appropriate for storage not for working with chemicals outside of their original containers The high setting provides protection for working with chemicals 4 Minimize Cross Drafts and Eddy Currents Air flow into the fume hood is adversely affected by cross drafts and
55. e to be used Various suppliers have similar labels UW Environmental Health and Safety 2 13 Change 1 July 2010 Laboratory Safety Manual Section 2 Chemical Management Figure 2 1 UW Hazard Label Product Name Carcinogen Sensitizer Allergen Health Corrosive Toxic Highly Toxic Irritant Long Term Effects Flammable Combustibie lammabilit Self Ignitin Promotes Fire Pyrophoric Oxidizer Reactivit Organic Peroxide Explosive Water Reactive Unstable Reactiv Target Eee Blood Cadiovascular Organs Equipmen Eyes Apron Gastrointestinal Dust Mask Kidney Face Shield Liver Fume Hood Mutagen Gloves Glove Box Nervous System Goggles Reproductive Systems Lab Coat Respiratory System Respirator Skin Safety Glasses Other Other b Handwritten Label Handwritten labels as shown in Figure 2 2 may be used to label additional containers The container s contents must be identified and the chemical s hazards must be described EH amp S recommends that you also add the name and date associated with the container to help with chemical management Figure 2 2 Handwritten Label ACETONE Flammable Skin eye and respiratory tract
56. ed prior to disposal UW Environmental Health and Safety 4 3 September 2009 Laboratory Safety Manual Section 4 Laboratory Equipment and Facilities protect the contents from extreme temperatures for a limited time Contact EH amp S at 206 543 0465 for further information on flammable liquid storage cabinets a UL or FM Approval Flammable liquids should be stored in an Underwriter s Laboratory UL listed or Factory Mutual FM approved flammable liquid storage cabinet outfitted with approved automatic or self closing doors All new cabinets must have UL or FM approval Note Some existing wooden cabinets that are not labeled with UL or FM approval are still in service and approved for use b Label Cabinets must be labeled Flammable Keep Fire Away c Capacity Do not over fill cabinets Check manufacturer s recommendations for storage limits d Bottles All bottles should be placed on the shelves never stacked Keep all containers tightly closed e Incompatible Chemicals Do not store incompatible chemicals in these cabinets 1 Cabinet Doors Cabinet doors should never be propped open unless the mechanism is a designed part of an approved cabinet g Secondary Containment There should be a secondary containment on each shelf and at the bottom of the unit These plastic or rubber trays retain spills h Unapproved Storage Tops of cabinets are not storage shelves Do not store combustible materia
57. eddy currents Cross drafts occur when people walk in front of a fume hood or when nearby windows or doors are open Eddy currents occur around the person using the fume hood and around objects inside it To limit these effects fume hoods should not contain unnecessary objects and the slots within the fume hood which direct air flow must not be blocked The slot at the rear of the work surface is essential for proper air movement If large pieces of equipment or large numbers of bottles are placed in front of the slot they should be raised up on blocks or placed on a shelf to allow air to flow into the slot Equipment should be placed as far to the back of the fume hood as practical Work should be performed at least six inches inside the fume hood opening to prevent cross drafts and eddy currents from pulling contaminated air out of the fume hood and into the room 5 Sliding Sashes The sash should be kept as low as possible to improve overall performance of the hood The more closed the sash is the better protection from an unexpected chemical reaction Procedures should be done with the sash at the level UW Environmental Health and Safety 4 8 Change 1 July 2009 Section 4 Laboratory Equipment and Facilities Laboratory Safety Manual of the maximum approved sash height marking or lower Use a separate safety shield such as a face shield when working with an open sash Chemical Evaporation It is illegal to evapor
58. edule with your local Facilities Services to remove materials or equipment that are attached to the building or would impact building materials Refer to the Laboratory Safety Manual Appendix F for contact means a Decontaminate your laboratory equipment if it has or may have come into contact with hazardous materials Follow the instructions and fill out Form UoW 1803 Notice of Laboratory Equipment Decontamination and attach it to the equipment For more details see the form at http www ehs washington edu forms fso lab_equip pdf To schedule pickup or drop off of surplus equipment see http Awww washington edu facilities transportation movingandsurplus Freezers special arrangement must be made with EH amp S to move freezers and Dewar flasks that contain infectious materials Specialized moving companies can move other materials See Section 10 of this manual for more details General a Inform vendors and on campus suppliers of your new box number and physical delivery address Update your own information on www myuw washington edu Follow guidelines on records retention in Section 7 of this manual and also on the Records Management website at http Awww washington edu admin recmgt index php Box and label sensitive files data patent files etc for personal transport UW Environmental Health and Safety 23 Change 1 July 2010 Laboratory Safety Manual Appendix E Checklists G SOP REQUIRED ELEMENTS CHECKL
59. emical products can be purchased without restriction from suppliers through eProcurement on line ordering system or through UW Purchasing Services However the following rules and guidelines apply to some chemicals 1 Hazardous Chemicals Order only the amount of chemicals needed Many manufacturers will supply smaller quantities or containers if requested by the purchaser Do not stockpile chemicals Chemicals that are expired and or appear to be no longer useful are considered hazardous waste Purchase hazardous chemicals in plastic coated bottles when available instead of uncoated glass bottles If possible hazardous chemicals should be received directly by the laboratory If it is received in an office there should be a safe location such as a designated table with adequate open space reserved for temporary storage of the package When you open a shipment you should verify that the proper chemical was sent that the container is intact and that the label is legible The date of receipt should be written on the container s label UW Environmental Health and Safety 2 7 Change 1 July 2010 Laboratory Safety Manual Section 2 Chemical Management 2 Pharmaceuticals Pharmaceuticals not regulated by the Drug Enforcement Administration DEA e g antibiotics heparin sterile water and over the counter drugs can be purchased through UWMC Drug Services Some restrictions may apply For more information and to see
60. erty by fire Best known on the industrial scene for the National Fire Codes 16 volumes of codes standards recommended practices and manuals developed and periodically updated by NFPA technical committees Among these is NFPA 704 It contains the code for showing hazards of materials using the familiar diamond shaped label or placard with appropriate numbers or symbols National Institute for Occupational Safety and Health A research agency within the Public Health Service U S Department of Health and Human Services DHHS which among other activities tests and certifies respiratory protective devices recommends occupational exposure limits for various substances and assists OSHA in occupational safety and health investigations and research http www cdc gov Niosh homepage html National Toxicology Program A group within the U S Department of Health and Human Services which produces the Annual Report on Carcinogens Spastic involuntary motion of the eyeballs in a horizontal rotary or vertical direction Relating to the sense of smell UW Environmental Health and Safety 19 Change 1 July 2010 Laboratory Safety Manual Appendix E Checklists F LABORATORY MOVING OUT CHECKLIST Use this checklist as a tool to help you relocate or shut down your laboratory or to temporarily relocate for remodels and renovations Refer to the Laboratory Safety Manual Section 10 Moving In Moving Out for more details i
61. es etre eR REP HUN ena ERR Sc a 3 6 7 Inherently Waste like Chemicals pee e ee deben ined Wa aee Pee Eee eet 3 8 D HAZARDOUS WASTE COLLECTION REQUESTS eese nennen eene eene eere 3 8 1 Hazardous Waste Collection Overview eese eene nennen een rennen tenete nennen nenne 3 8 2 Collection Requests ONE TIME orcs sii dent E RR eg e t etl pe 3 9 3 Routines and Routine Collection Requests esee ener eene eee nennen trennen 3 9 4 W ste Cleanouts n doe dE O EEG EG Pe re HG ewe pe Dope ETNEA 3 9 5 What Happens to Hazardous Waste nene trennen 3 9 E S TRASH DISPOSA mene ee EUR RU e RU eh Ote 3 10 Lh Trash Disposal of Chemicals hs Ra cnr eme de d arbe ree phen deut 3 10 2 Trash Disposal of Empty Chemical Containers eese eene entente nennen 3 10 3 Trash Disposal of Contaminated Items eese eene trennen rene eene ener nnne 3 11 E SEWER DISPOSA 43r otia eer t e er n et e eie ien 3 11 1 King County Local Sewer Discharge Limits eese eene nennen nennen rennen ene 3 11 2 Outside King County out ee ide Gr eitis tpe ees Rte oe reb eese ede ne 3 12 3 Chemical Treatment Log ooa RR PU RENE RR HER BERE HERR Eee 3 12 Page iii Laboratory Safety Manual Change 1 July 2010 4 Soaps Bleach and Acetone aede te eii et ee eve e eR e Eye Rete ex wee
62. eted UW Hazardous Waste Labels on each waste container not needed for containers with an original label and original contents Consider the MyChem Chemical Exchange for your unwanted but useable chemicals Useable chemicals are unexpired and preferably unopened Finally remember to update your chemical inventory in MyChem 5 What Happens to Hazardous Waste EH amp S has a Waste Minimization Program that reuses recycles and treats more than 5096 of the total waste generated at the University of Washington Reuse recycling and treatment takes place both in laboratories and at the EH amp S hazardous waste facility Some waste streams like batteries paint and oil are sent offsite for recycling by contractors For more information see the Waste Minimization subsection below or visit http Awww ehs washington edu epohazreduce index shtm All hazardous waste at the University of Washington that is not reused recycled or treated is sent to permitted hazardous waste recycling and disposal facilities Flammable waste is used as an alternative fuel to incinerate hazardous waste Most of the other waste streams are incinerated at high temperature A few waste streams are placed in permitted hazardous waste landfills UW Environmental Health and Safety 3 10 Change 1 July 2010 Section 3 Chemical Waste Management Laboratory Safety Manual E TRASH DISPOSAL 1 Trash Disposal of Chemicals The following are prohibited i
63. f the principal potential hazards of working in confined spaces A disease characterized by recurrent attacks of dyspnea wheezing and perhaps coughing due to spasmodic contraction of the bronchioles American Society for Testing and Materials A voluntary membership organization whose members devise consensus standards for materials characterization and use ASTM 1916 Race Street Philadelphia PA 19103 215 299 5400 Neither causing nor exhibiting symptoms A loss of muscular coordination A wasting or diminution in the size of tissues organs or the entire body The minimum temperature to which a substance must be heated without application of a flame or spark in order to cause that substance to ignite See alkali The temperature at which a liquid changes to a vapor state at a given pressure Flammable materials with low boiling points below 100 generally present special fire hazards A slow heartbeat Pulse rate below 60 beats per minute Inflammation of the bronchial tubes in the lungs A substance capable in solution of neutralizing both acids and bases Clean Air Act The federal law enacted to regulate reduce air pollution Administered by the EPA The maximum allowable human exposure limit for an airborne substance not to be exceeded even momentarily Also see STEL and TWA A substance that causes cancer Also see select carcinogen An assigned number that identifies the material CAS stan
64. facturer approves the alternate chemical Records as to the design of the hood and the design chemical usage must be maintained in the laboratory UW Environmental Health and Safety 4 10 September 2009 Section 4 Laboratory Equipment and Facilities Laboratory Safety Manual Hood Approval The Principal Investigator must verify that the size shape and layout of the proposed hood as offered by the hood manufacturer is appropriate for the intended use The PI must also develop a management plan for the hood which addresses staff training procedures for using the hood including emergency procedures ongoing maintenance and certifications for the hood and recordkeeping This plan needs to assure continuity if management of the hood is taken over by another individual A description of the items required in the management plan is available from EH amp S 206 543 7388 Hood approval by EH amp S is contingent on submittal of the hood design information from the proposed manufacturer and submittal of the management plan Laboratory Staff Information and Training All personnel in the laboratory must be trained as to the fact that the ductless hood re circulates air back into the room that only certain designated chemicals may be used within the hood and that failure to properly operate and maintain the hood may result in personnel exposures Also a sign must be placed on the hood identifying what chemicals may be used and war
65. ffice a Usage records including Radiation Survey Records must be updated finalized and submitted to Radiation Safety Waste disposal records must be finalized and turned to the Radiation Safety Office a All radioactive material waste containers must be picked up by the Radiation Safety Office Personnel dosimeters must be returned to Radiation Safety Termination bioassays must be performed if necessary For questions or assistance call the EH amp S Radiation Safety Office at 206 543 0463 Transportation a Q Biological Materials follow the instructions in Appendix B of the UW Biosafety Manual online at http www ehs washington edu rbsbiosafe appendixb paf Chemicals follow the instructions in Section 10 Moving In Moving Out and in Section 2 Chemical Management in your UW Laboratory Safety Manual Under certain conditions you can transport the chemicals yourself on campus You can also arrange for a hazardous material contractor to pack and or transport your chemicals for you Radioactive Materials For short moves of radioactive materials between locations on the contiguous UW Seattle campus hand carrying is an option For transport of radioactive materials over public roads call the Radiation Safety Office Equipment and Non Hazardous Items you may choose to hire an outside moving company or UW Property amp Transport Services to move equipment Either way do these two items first a Sch
66. fore use Special Handling Procedures and Storage Requirements Are any special storage requirements for the chemicals noted This may include restricted access areas special containment devices and safe methods of transportation Spill and Accident Procedures Are spill or accidental release procedures identified Are there any specifications as to how big a spill could be safely handled who might be designated to clean up the spill and if any special spill clean up materials are needed Waste Disposal Are waste disposal procedures identified For more information refer to Section 3 of this manual If particularly hazardous substances are involved this is especially important Special Precautions if Using Animals Are procedures for safely handling the animal described If particularly hazardous substances are used in the process the following should also be present Definitions and partial listings of these acute toxicants carcinogens reproductive hazards highly dangerous chemicals and select toxins are in Appendix H of the laboratory safety manual Approvals Are training and approval requirements before someone can perform the procedure noted Decontamination Procedures Are special precautions identified for handling the especially hazardous materials B Designated Area Equipment Is a specific area and specific equipment for safe use of the hazardous material identified Name Title Signature Date
67. grity Chemicals that are expired in bad shape or no longer needed must be managed as hazardous chemical waste For more information about chemical waste management see Section 3 of this manual After completing the inventory the Chemical Hygiene Officer should print two copies of the inventory from MyChem one copy for the lab and one for home in case of an after hours emergency in the laboratory UW Environmental Health and Safety 2 6 September 2009 Section 2 Chemical Management Laboratory Safety Manual 3 Material Safety Data Sheets MSDSs Material Safety Data Sheets MSDSs are documents that describe the physical and health hazards of chemicals Manufacturers of chemicals must provide MSDSs for chemicals that they sell Although many MSDSs have limited application in laboratories due to their orientation towards industrial use of large quantities of a chemical they provide basic information that all persons using that chemical need to know MyChem is the University of Washington s centralized MSDS database for chemicals used by University personnel see Section B 1 above EH amp S maintains the MyChem MSDS database Laboratory staff and students must have ready access to MSDSs for all chemicals used in the laboratory The department or laboratory may choose whether to maintain the MSDSs in either electronic or paper format The source of the MSDS is less important than the requirement that all personnel using chemi
68. h you are expected to operate your laboratories New regulatory oversight and guidelines have been given to laboratories in institutions of higher education by agencies such as the Department of Homeland Security the Environmental Protection Agency the National Institutes of Health and our state Occupational Health and Safety program Maintaining your commitment and oversight in addressing the on going and new requirements that address the safe handling processing and disposal of chemicals will continue to be essential Prior to recycling your older manual remember to update and transfer your own laboratory specific information from your old manual to the new manual see the Quick Start Guide at the front of the manual If you have questions or need a new binder please call the Department at 206 543 7388 email Occupational Health and Safety at uwcho uw edu or visit our website at http Awww ehs washington edu A very sincere thank you in your various roles to keep the University of Washington an exemplary institution where promoting and practicing environmental health and safety principles are part of the University way of pursuing our educational research and service missions Denis Sapiro Manager Occupational Health and Safety Office Acknowledgments Environmental Health and Safety Department Editors Stan Addison MS CHP Manager Radiation Safety Office Stuart Cordts CIH NRCC CHO Health and Safety Su
69. ha gov dsg hazcom ghs html F TRANSPORTING CHEMICALS Avoid transporting chemical containers which may have contamination on the outside e avoid the need to wear gloves or other PPE while transporting chemicals If gloves must be worn either be escorted by another person to open and close doors and press elevator buttons or remove the glove from one hand and use it to open doors while holding the chemical in the other hand 1 Transporting between Floors and Buildings on Campus This section applies to transportation by hand or by cart In general when possible use freight only elevators when moving chemicals between floors a Moving a Single Chemical 1 2 The person doing the moving must be trained in the hazards of the chemical and know what to do in the event of a spill of that chemical The exterior of the container should be clean enough that it could be handled without the need for protective gloves Chemical bottles must be labeled and should be securely capped and placed in a bottle carrier Chemical containers that are glass and do not have closing caps or handles should be placed in bottle carriers or larger containers and surrounded by vermiculite or other absorbent material UW Environmental Health and Safety Laboratory Safety Manual a Nitrated Compounds Page 2 19 Change 1 July 2010 Section 2 Chemical Management Nitrated organics and inorganics constitute the largest class of compounds
70. hazardous waste by law The definition for extremely hazardous wastes is on the EH amp S website at http www ehs washington edu epowaste ehw shtm Also if your chemical is a known or suspected carcinogen such as those listed in Appendix H of this manual EH amp S strongly recommends that you triple rinse the container If you choose to dispose of the empty container do the following e Dry the empty container preferably in a fume hood Ensure that there are no sources of heat or open flame in the fume hood when drying containers that contained flammable chemicals e With a pen or marker cross out or black out the labels on the container e Leave the container uncapped Throw the cap away separately e Ifthe container fits in the trashcan place it there If it does not fit in the trashcan place it next to the trash UW Environmental Health and Safety 3 11 Change 1 July 2010 Laboratory Safety Manual Section 3 Chemical Waste Management e Donotleave empty containers in public areas such as hallways or loading docks unless you have made an agreement with Custodial Services or EH amp S for pickup services Consider reusing the empty container for accumulation of waste for that same chemical or other compatible chemicals If you do reuse a container deface or remove the label on the container and then fill out and affix a hazardous waste label to the container Defacing and labeling are required by law and also hel
71. he sink and sometimes supersede Hazardous Waste rules Please also refer to the local sewer limits rules in Section F below 9 Waste Evaluation Request If you are unsure whether your waste is hazardous please submit an online Waste Evaluation Request Fill out all information completely and attach the MSDS s for the chemical s to it EH amp S staff will evaluate your waste stream for you and advise you on proper disposal C HAZARDOUS WASTE ACCUMULATION RULES Follow the below rules for hazardous chemical waste accumulation 1 Appropriate Containers Accumulate waste in an appropriate container compatible with the waste You may reuse containers even containers that were used for other chemicals if they have been rinsed and the original labels have been defaced note that the rinseate may be hazardous waste according to the definitions in Section B above Containers that were designed for solid chemicals should not be used for liquids Use only containers that show no sign of damage or deterioration You must use containers with screw top closures The lids of waste containers should be removed only when waste is being added to the container Use spring loaded funnels for adding waste frequently to waste containers Finally do not fill the containers completely Each container must have at least a one inch of headspace above the waste when it is collected Request collection of your waste ahead of time to avoid overfilling your co
72. hemicals used in your laboratory see Chapter 296 841 WAC Airborne contaminants Continued 100 2 09 10 1 800 4BE SAFE 1 800 423 7233 1 Change 1 July 2010 Laboratory Safety Manual Appendix B Glossary Environmental Health and Safety 44429 UNIVERSITY of WASHINGTON Appendix B Glossary This glossary contains common terms found in the Laboratory Safety Manual and on Material Safety Data Sheets Another valuable source for information about MSDS entries can be found at the web site http www ilpi com msds ref index html absolute ACGIH acids action level acute health effect acute toxicity acutely hazardous A chemical substance that is not mixed pure For example Absolute Alcohol ethyl alcohol containing not more than one percent by weight of water American Conference of Governmental Industrial Hygienists Incorporated An organization of professional personnel in governmental agencies or educational institutions engaged in occupational safety and health programs ACGIH develops and publishes recommended occupational exposure limits see TLV for hundreds of chemical substances and physical agents annually ACGIH 1330 Kemper Meadow Drive Cincinnati 45240 1634 513 742 2020 http www acgih org home htm Any chemical which undergoes dissociation in water with the formation of hydrogen ions Acids have a sour taste and may cause severe skin burns Acids
73. highly toxic gases in a gas storage cabinet may be required as described in earlier paragraphs in this section UW Environmental Health and Safety 2 30 September 2009 Section 2 Chemical Management Laboratory Safety Manual UW Environmental Health and Safety Laboratory Safety Manual Page 3 1 Change 1 July 2010 Section 3 Chemical Waste Management Environmental Health and Safety 4244 UNIVERSITY of WASHINGTON Section 3 Chemical Waste Management Contents A HAZARDOUS CHEMICAL WASTE RESPONSIBILITIES 3 3 1 Laboratory Worker S nennen 3 3 2 UW EH amp S Environmental Programs 3 3 B WHAT QUALIFIES AS HAZARDOUS WASTE 3 3 1 Flammable lgnitable sedre e tr rte rettet 3 3 2 COITOSIVG eta eie nage aduentu uev epa age eu de 3 4 o REACIVE isi cV RH M i es 3 4 4 STOXIO cat ertet 3 4 2 seta xe sorde Ee et 3 5 a Halogenated Organic Compounds 3 5 b Polycyclic Aromatic Hydrocarbons 3 5 6 Carcinogenic i eee Ene e ee p ine 3 5 7 Trash R les eerte eiae dtes oed 3 6 8 Local Sewer Limits sess 3 6 9 Waste Evaluation 3 6 C HAZARDOUS WAS
74. icing the requested area or equipment if it has not been decontaminated and or cleaned Conditions which can lead to service rejection include such things as visible debris from absorbents or glassware diapers or papers taped to surfaces which were supposedly decontaminated and cleaned and visible or sticky spilled materials If the laboratory is expected to be unattended when service personnel arrive an informal note should be left stating a contact name and phone number in case there are questions about the work area or if equipment needs to be moved H DECONTAMINATION OF EQUIPMENT FOR DISPOSAL Laboratory equipment is often contaminated with hazardous materials and or may be inherently unsafe UW Surplus Property cannot accept some types of laboratory equipment and cannot accept laboratory equipment containing hazardous materials To surplus contaminated or potentially contaminated laboratory equipment you must first make sure that the equipment is safe for handling and resale by following the directions on the Notice of Laboratory Equipment Decontamination UoW 1803 at http www ehs washington edu forms fso lab equip pdf The Chemical Hygiene Officer Laboratory Supervisor or Principal Investigator must sign this notice to certify that all of the applicable instructions on this form have been followed Affix this notice to the equipment Surplus Property will not pick up equipment that does not have this notice attached or does n
75. include expired chemicals chemicals in deteriorating containers and chemicals that appear to be or are unusable State inspectors may issue fines or infractions for inherently waste like chemicals in your laboratory Do not keep chemicals past their expiration date and conduct cleanouts when you do your annual chemical inventory update Please also see the section on legacy chemicals in Section G 3 below Legacy chemicals are those that are left behind by laboratory staff when they leave the university or move laboratories They become the responsibility of the new space occupants D HAZARDOUS WASTE COLLECTION REQUESTS 1 Hazardous Waste Collection Overview EH amp S collects hazardous chemical waste from all UW campuses and UW owned and operated facilities There are about 3500 laboratories on and near the Seattle campus Therefore EH amp S UW Environmental Health and Safety 3 9 Change 1 July 2010 Laboratory Safety Manual Section 3 Chemical Waste Management may only be near your area once every week or every other week Therefore you may have to wait one to several weeks after you send your collection request before EH amp S collects your waste To avoid problems plan ahead and request collection before your containers are full 2 Collection Requests One Time Request collection of your waste by submitting a Chemical Waste Collection Request found online at http www ehs washington edu forms epo 1470 pdf
76. increased if fire sprinklers protect the building or in some cases if hazardous materials are in approved cabinets Limits in control zones above the second floor are reduced by the fire code and the higher the floor the greater the reduction Researchers and other building occupants must cooperate with each other to make sure that hazardous material quantities do not exceed code limits This can be aided by maintaining an accurate chemical inventory in MyChem Some specific quantity limits per control zone are listed in the following table Table 2 2 Example Hazardous Material Quantity Limits This table is not complete and there are also many additional criteria in the implementation of the limits To assure compliance with the IFC contact the EH amp S Building and Fire Safety Office at 206 543 0465 Table 2 2 Example Hazardous Material Quantity Limits Material Quantity Limits IFC Comments per Control Zone Citation Class I A Flammable 30 gallons 2703 1 1 Limits increased if stored in approved Liquids cabinets or the zone is sprinklered Combination Class 1 1 120 gallons 2703 1 1 Limits increased if stored in approved Flammable Liquids cabinets or the zone is sprinklered Flammable Gas 1000 cubic feet 2703 1 1 Limits increased if stored in approved cabinets or the zone is sprinklered Organic Peroxide Class 5 pounds or more 2703 1 1 Limit depends on class to V Pyrophoric 4 pounds 2703 1 1
77. ion survey of all accessible surfaces of the equipment with an appropriate instrument If you detect radioactive contamination you must clean the equipment with small amounts of warm detergent water Avoid splash Blot dry with paper towels Commercial radiation decontamination solutions containing chelating agents may be helpful Resurvey to assure that contamination is less than 100 counts per minute per 100 square centimeters of surface If contamination persists or you have other questions contact the EH amp S Radiation Safety Office at 206 543 6328 3 Equipment Used to Process Store Biological Material Remove all biological material from the equipment Decontaminate with a 1 10 bleach solution After 30 minutes of contact time rinse metal surfaces If you have specific biosafety questions contact the EH amp S Research and Biological Safety Office at 206 221 7770 Before repair or relocation biological safety cabinets must be decontaminated by EH amp S or by a contractor approved by EH amp S For this service contact EH amp S at 206 543 0465 UW Environmental Health and Safety Department of Labor amp Industries Hazardous Chemicals 4 Chapter 296 828 WAC September 2010 Edition Department of P EON Ky LABOR H ie Q 7 414 135 000 09 10 printing Hazardous Chemicals in Laboratories WAC 296 828 100 Scope This Chapter applies to the laboratory use of hazardous chemicals To determine
78. l decontaminated by EH amp S For additional information on the proper use of BSCs Class II type B2 design cabinet certification troubleshooting problems or decontamination please contact EH amp S at 206 543 0465 6 Laminar Flow Hoods Laminar flow hoods are designed to protect the work surface from contaminants and blow out into the face of the person using the hood Therefore any chemical use will cause the person to be exposed to the chemical Toxic volatile chemicals may not be used in a laminar flow hood Ductless Laboratory Hoods In some cases installation of a ducted fume hood may be impossible and installation of a ductless hood is requested for approval by EH amp S This type device uses special filters or absorbents to clean the contaminated air in the hood prior to recirculating the air back into the room Recirculation of potentially contaminated air into the room presents special dangers and special requirements must be met The requesting department must demonstrate that the following concerns are addressed as long as the hood is in use a Chemical Characterization Each of the chemicals to be used in the ductless hood must be completely characterized as to the quantity which may be released within the hood at one time and the frequency of use The hood manufacturer will need this information for the design of the hood Once designed use of other chemicals in the hood must be forbidden unless the hood manu
79. led For the last five years the UW reused recycled or treated about 4096 of our hazardous chemical waste This section outlines some of the basic elements of this effort and how you can participate More information is at http www ehs washington edu epohazreduce index shtm There you will find an extensive and detailed list of services and resources 1 Chemical Procurement and Chemical Exchange Purchase only what you ll use especially if you re purchasing a hazardous chemical One recent study suggested that up to 40 of the hazardous waste produced by laboratories is actually unused and expired chemicals Shop for free chemicals in the MyChem Chemical Exchange Or if you have chemicals in good condition that you do not need consider listing them in the MyChem Chemical Exchange For more information see the EH amp S website at http Awww ehs washington edu eporecycle chemex shtm 2 Treatment and Recycling in the Laboratory You are encouraged to treat or recycle your own waste EH amp S staff are available to help you get started and in some cases offer free materials for recycling and treatment Please see http Awww ehs washington edu epohazreduce index shtm for more details UW Environmental Health and Safety 3 15 Change 1 July 2010 Laboratory Safety Manual Section 3 Chemical Waste Management 3 Hazardous Materials Recycling Both EH amp S and UW Recycling Property Transport and Services manage the
80. les APS 15 01 html 5 Radioactive Materials The State of Washington Department of Health Division of Radiation Protection licenses radioactive materials use Using radioactive materials requires the prior approval of the EH amp S Radiation Safety Office Orders for radioactive materials must be placed through the UW Purchasing Department 6 Highly Dangerous Materials Materials that are extremely hazardous to property health or the environment explosives pyrophoric materials highly water reactive chemicals and highly toxic gases for examples must not be procured until the necessary permits and administrative engineering and environmental controls are in place Hazardous materials must be stored and used in accordance with numerous regulations including but not limited to the International Fire Code and local amendments See Section G Special Chemical Hazards below for examples Contact the EH amp S Building and Fire Safety Office at 206 543 0465 for more information 7 Compressed Gas Cylinder Procurement Whenever possible gas cylinders should be purchased through the preferred supplier Praxair to ensure that the supplier has a cylinder return authorization program Please refer to the UW eProcurement web site http Awww washington edu admin stores eprocurement Contact information concerning Praxair is also available on the EH amp S web page http Awww ehs washington edu fsohazmat gascylinders shtm If a different ven
81. lete Exposure Information isi eee hr rhe rr e RR Ro eed LRL 6 3 2 Other Obsolete Documents neuste ete dee eet e RE dee ere rette ROSE 6 3 3 Records from Decommissioned Laboratories eese nennen nennen rennen 6 3 Cy EH amp S RECORDS genesi eee ORI eee pa RES 8 3 4 Records Concerning Individuals uae t ote tar ee i epe 6 3 2 Centralized c RERO RU Oeo RE RES 6 3 SECTION 9 EMERGENCY PREPAREDNESS AND RESPONSE eese esee entes ee snot tn snas tn sna ensn 9 1 A BEFOREAN EMERGENCY 4 eet otii eee n et ei Roco veces doeet eset ds 9 2 L Departmental Plans and Policies e ee qa ar uie e voee eb eee eee ees 9 3 2 Planning and Prevention ies estet te SP TREE Ree ge e ehe EAEE a OEA EDAEN 9 5 3o Spill First Ald rte eh ERR e SERE D ERR TE 9 8 4 Fire Extinguishers Eyewash Stations and Safety Showers eere eene 9 10 3 Securing Equipment and Supplies eio dete ede eter ede eere Ed te odii e Des 9 12 Training Staff for Emergencies 9 12 B RESPONSE TO SPECIFIC INCIDENTS ACCIDENTS sees nennen rennes 9 13 1l Accidents Resulting in Personal Injury or Exposure eese eene een eene enne 9 15 2 Fires and Explosi ns i ae te er ta Reo EE Perte ESTE eruere het 9 14 Jc iss een ER
82. line at http www ehs washington edu forms epo peroxideguidelines pdf Chemicals containing more than 10 parts per million ppm peroxides must be deactivated before they will be collected by EH amp S Treatment methods are available through EH amp S email chmwaste u washington edu to obtain more information about them If the chemical is expired very old or otherwise poses great risk to laboratory workers an outside contractor will perform deactivation and stabilization services at the expense of the laboratory b Picric Acid and Other Polynitroaromatic Compounds Polynitroaromatic compounds are commonly used in laboratories and are safe in the form in which they are sold They are ordinarily sold with 3 to 1096 water added to stabilize them However they will become explosive if allowed to dry out Dry polynitroaromatic compounds must be wet with 1096 water before they can be collected by EH amp S UW Environmental Health and Safety 3 14 Change 1 July 2010 Section 3 Chemical Waste Management Laboratory Safety Manual c Sodium Azide Sodium azide although not inherently unstable can form highly explosive heavy metal azides if contaminated or used improperly Do not pour sodium azide into the sanitary sewer Disposal of sodium azide solutions to the sewer can cause the formation of lead or copper azides in plumbing Routine sewer disposal of sodium azide has caused several serious explosions d Nitrocellulose
83. ls on or beside these cabinets 2 Flammable Storage Refrigerators Flammable chemicals or chemical mixtures that need to be stored below room temperature must be stored in U L listed Flammable Material Storage Refrigerators or Freezers These refrigerators and freezers are specifically designed by the manufacturer to have non sparking interiors All laboratory refrigerators and freezers must be prominently labeled with a warning sign indicating whether it can be used for flammable or non flammable storage For these warning signs or information regarding a Flammable Storage Refrigerator purchase contact EH amp S at 206 543 0465 For more information on flammable storage refrigerators see http Awww ehs washington edu fsofire flamfrig shtm UW Environmental Health and Safety 4 4 Change 1 July 2010 Section 4 Laboratory Equipment and Facilities Laboratory Safety Manual C LABORATORY SIGNS Laboratory signs may be either permanently mounted or mounted temporarily as described in Section 2 A 7 A synopsis of mandatory and desirable signs is provided in the following table and explanatory material is described in the following paragraphs Table 4 1 Safety Related Signs Description of Sign Mandatory For more information see Emergency contacts phone numbers Mandatory Section 4 C 1 Laboratory floor plan See Section 4 C 2 Section 4 C 2 Emergency safety equipment location signs
84. n nenne enne ene 4 4 4 Food and Drink Prohibitions etr eee mor e a ear 4 4 2 reg and Equipment Warning Sece en o DU eg ep RN ee HT EE 4 4 National Fire Protection Agency Signs essent 4 5 74 LabsSpecific Signs Aci e e eee dea eee te ee Rl deett aee 4 6 D LABORATORY VENTILATION eot oe Ee pedet FE 4 6 T cLaboratory Desteniad s oquut suc eet pea dtu et ut etu etat tete s 4 6 2 Hoods ae t HERO EMEN Qu a PEN HEUS EAE OSEERE EN 4 7 32 Perchloric Fume Hoods ie tse eese E e ect eere sduagss lana bine eye qe eve Pe pue 4 8 LEE CITTA TEE 4 9 3 4 Biological Safety Cabinels is esee SU ae aos ed RE RERO RE 4 9 6 Laminar Flow Hoods sistens R ee e ede pese d ede de d ep te eoe 4 9 7 JDu uctless Laboratory Hoods ise e dee e HR ee fe e e He d Pre ee Pe tet de dente 4 9 8 Cold Rooms Warm Rooms and Environmental Chambers 4 10 9 Other Ventilation Systems site ertet tee de etre RD rte es 4 10 10 Maintenance of Ventilation Systems tssiri is eien norin nene teen 4 11 E OTHERFACILITY CONDITIONS pen eee pde 4 11 1 General Laboratory Environment eee 4 11 Page iv Change 1 July 2010 Laboratory Safety Manual 2 Electrical Haz rds a iie te eee deat edi re gu e veter be ee e d ge eevee 4 14 3 FPock Out Iag Out
85. n stickers can be obtained from EH amp S 5 Area and Equipment Warnings Operation and warning signs and labels must be posted on such things as alarm systems biosafety cabinets fume hoods sash opening height Warnings may also need to be posted in areas or on equipment where special or unusual hazards exist such as biohazards lasers magnetic fields radioactive materials high voltage restricted access or particularly hazardous substance control areas These signs may be mandatory depending on the degree of hazard and possibly local codes UW Environmental Health and Safety 4 7 September 2009 Laboratory Safety Manual Section 4 Laboratory Equipment and Facilities b Vents Do not block or cover supply and exhaust vents Occupant changes to lab ventilation may compromise the safety features of the laboratory and local exhaust systems such as fume hoods biosafety cabinets etc 2 Fume Hoods A fume hood is ventilation equipment that vents separately from the building s heating ventilation and air conditioning HVAC system The primary means of controlling airborne chemical exposure is a fume hood Fume hoods should be used when working with toxic compounds or compounds with a boiling point below 120 C However some aqueous solutions may be an exception to this rule It may be necessary to use a closed system such as a glove box or bag for highly hazardous chemical materials EH amp S maintains a roster of fum
86. n the trash because of their chemical or physical hazards e Hazardous chemical waste as defined earlier in this section e Known probable or suspected carcinogens irritants and sensitizers see a current MSDS for the chemical to determine if the chemical is any of these e Free liquids of any type e Pressurized vessels including aerosol cans e Laboratory glass and sharps e Radioactive waste e Batteries e Mercury including broken empty thermometers e Biohazardous waste To throw away chemicals that are not prohibited in the trash deface any labels securely double bag it and label it non hazardous so that custodial staff know it is safe for them to handle the trash 2 Trash Disposal of Empty Chemical Containers Empty chemical containers may still contain enough chemicals in them to present a hazard to custodial staff On the other hand it can be difficult to completely empty a container The legal interpretation of the word empty acknowledges this difficulty A container is legally empty when both of the following are true e Contents have been removed by normal no nonsense means such as inverting and draining shaking scraping or scooping and e than 3 of the contents remain If the chemical is extremely hazardous waste or a pesticide marked with danger or warning labels then the container must be triple rinsed before it is legally empty The rinseate from this process is also considered
87. n tns tn statu sens tatu 3 1 A HAZARDOUS CHEMICAL WASTE RESPONSIBILITIES eese eene enne 3 3 1 L bordtory Worketszs atit ee ete ie e EG RET eee cis irte eee ER De toes 3 3 2 UW EH amp S Environmental Programs Office eese enne nennen nennen trennen rennen ene 3 3 B WHAT QUALIFIES AS HAZARDOUS WASTE n a trennen rennen 3 3 l JFlammable Ignitable aee Re We een e ere ire e E ee e De o dee pb ge 3 3 Z2 PCOPTOSIVE itu E Deed deu tege e BIN ATR e RR edes 3 4 3 JRea ctive iode rete pe ERREUR UNI ER PH re re ads 3 4 C EIL E 3 4 ME T uj a ES 3 5 O C rcilnogenio eee HER THE De YE IURE OO EU E De Pee eye be tte shes Eee E eee 3 5 3 6 6 Local Sewer Limits c RR RU USES d aee RR URN dies 3 6 9 Waste Evaluation Request eet Re ee Reve e e iet E EYE Doa RE shee tiet 3 6 C HAZARDOUS WASTE ACCUMULATION RULRES eese nennen een eene nennen 3 6 l Appropriate Containers econtra rede EU EP Feo EP tpe get Ere geh 3 6 2 Hazardous Waste Labels dnbie Reine t eai 3 6 3 Location arier E 3 7 45 iSepregation icss gea A ethan a AA E petet yu tle e diee O RTE cy 3 8 3 Accumulation Volume Limits ege t a iea Rap ere DERES 3 8 Large Containers Drums s isse e
88. ncluding your responsibilities Laboratory Decontamination and Cleanout a If you are partially or completely vacating your laboratory for remodeling relocation or closure you must leave it clean empty and safe for Facilities Services staff or the next occupants Follow all applicable instructions on the Notice of Laboratory Moveout UoW 1800 online at http Awww ehs washington edu forms fso 1800 pdf The Principal Investigator or laboratory manager Chemical Hygiene Officer must sign the checklist to verify that all instructions were followed A copy of the Notice for Laboratory Moveout must be posted inside the door near one or more exits of your laboratory for Facilities Services or the next occupants Chemical Safety a Arrange for disposal of all hazardous waste and unwanted chemicals Attach a completed UW Hazardous Waste Label to any waste not in its original manufacturer s container and complete and send a Chemical Collection Request form UoW 1470 at least one month before you vacate a Properly manage unwanted gas cylinders Return gas cylinders to the supplier or to whom you are leasing them from if at all possible If you cannot do either email chmwaste u washington edu for assistance For questions or assistance call the EH amp S Environmental Programs Office at 206 616 5835 Biological Safety a If your laboratory is relocating or shutting down contact the EH amp S Research and Biological Safety Office RBSO at
89. ner een eene nennen trennen 1 4 2 Department Chair or Director siena eere pe reete to He dete ete ed ege peo Dog ea re EOKA EEDE ET en 1 8 3 Employees Students oie iet Rei rn ette RR RR 1 8 4 Environmental Health and Safety Department esee eene nennen 1 9 5 UW Chemical Hazards Advisory Committee CHAC eese teen nennen rene 1 9 6 UW Chemical Hygiene Officer UW CHO 1 10 SECTION 2 CHEMICAL MANAGEMENT eere eene te ennt tn snnt ta snas tatus ossa sosta 2 1 BASIC LABORATORY SAFETY PRACTICES nennen rennen nete 2 1 1 WorkingAlofle iet ie eed gere e re i EIS EO pter bete ie cedens 2 1 2 Prevent esie n red a vaste DESE E NEER Ee EE E V EREE ES 2 1 3 Washing Hands sU PRO a A oe A dat ed ee 2 1 4 Food and Drink ce Batra ive ates Biv ede ius niei ie ie de ene 2 2 Jo Vacuum ssa ete oet e cR Ue ete dee d aet date 2 2 Access to Emergency Exits and Equipment nennen nennen trennen rennen ene 2 2 Z iL bordtoOry us eset RT REPERI EHE ERE S DH tha RD 2 2 B CHEMICAL INVENTORY AND MATERIAL SAFETY DATA SHEETS I 2 2 12 Access to My Chem e n e SE ed e de OR E tet le ER Ee BP Eee 2 3 2 Conducting your Chemical Inventory eee eese
90. ning that the air is re circulated back into the room from the hood 8 Cold Rooms Warm Rooms and Environmental Chambers a Room Design Controlled environment rooms generally are completely enclosed with no fresh air and heating cooling and other environmental systems independent from the building Rooms large enough to enter should be designed or retrofitted with doors that allow anyone trapped inside to get out easily The electrical system within environmental rooms should be independent of the main power supply so that people are never left in these areas without light Chemical Use Controlled environment rooms usually re circulate the air using a closed air circulation System Hazardous chemicals must not be stored in these rooms because ambient concentrations of volatile chemicals can accumulate to dangerous levels Flammable solvents should not be used in controlled environment rooms Ignition Sources in these rooms could ignite vapors Avoid using volatile acids in cold rooms because vapors can corrode the cooling coils leading to possible refrigerant leaks If solid carbon dioxide dry ice is placed into a cold room its sublimation will raise the carbon dioxide levels within the room possibly to dangerous levels Use extra precautions if you must use or store dry ice in these spaces 9 Other Ventilation Systems A ventilation engineer must design all other local exhaust systems used in the laboratory Do not attach ca
91. nopy hoods or snorkel systems to existing fume hood exhaust ducts without consulting a ventilation engineer at the Seattle campus Facilities Services Campus Engineering 206 543 7372 or your local campus engineering design services if available local exhaust systems should have a visual indicator that the system is functioning properly at all times even if the indicator is just a Kimwipe UW Environmental Health and Safety 4 17 September 2009 Laboratory Safety Manual Section 4 Laboratory Equipment and Facilities 1 Custodial Services UW Custodial Services will clean floors in laboratories only if requested Contact Custodial Services at 206 685 1500 on the Seattle campus and refer to Appendix F for contact numbers for Facilities Services at other locations Custodial floor care equipment should not be used to clean up spills or chemicals 2 Servicing of Lab Area or Equipment To protect maintenance and facility workers any laboratory area or equipment needing servicing is required to be unobstructed emptied of chemicals decontaminated with a decontaminating chemical as needed washed with warm soapy water and rinsed The area or equipment must have a signed Notice of Laboratory Equipment Decontamination UoW 1803 attached before service will be provided This form is available online at http Awww ehs washington edu forms fso lab_equip pdf Facilities Services and maintenance personnel are trained to reject serv
92. nsitive and will detonate with the slightest movement or vibration Do not allow picric acid to contact metal that is readily oxidized or be stored in a container with a metal cap Lead iron and copper metals are particularly dangerous due to metallic picrate formation b Organic Peroxide Forming Solvents Organic peroxide forming solvents become shock sensitive when allowed to oxidize and form appreciable quantities of explosive peroxides Most of these solvents are also flammable Most peroxide forming solvents are colorless mobile liquids Oxidation can occur when the solvent is exposed to atmospheric oxygen This reaction is catalyzed by light as well as by temperature and pressure changes The additional precautions you take to control peroxide forming hazards described in the opening paragraph of Section G above and in this section need to be documented in your SOPs Below is a list of good laboratory practices For more information see the Peroxide Forming Chemicals Management and Assessment Guidelines online at http Awww ehs washington edu forms epo peroxidequidelines pdf 1 Highly Concentrated Peroxides Over a period of time peroxide concentrations can increase to hazardous levels Solvents with high concentrations of peroxides will appear viscous or contain needle like crystals If peroxides are visible no further handling is recommended Contact EH amp S at 206 616 5835 for assistance with professional testing and st
93. ntainers 2 Hazardous Waste Labels Label the container using the Hazardous Waste Label Figure 3 1 below UW Environmental Health and Safety 3 7 September 2009 Laboratory Safety Manual Section 3 Chemical Waste Management Figure 3 1 Hazardous Waste Label HAZARDOUS WASTE FEMA MPO w AND RIGOCIA HAZARI zs TEGENERATOR 5 ION Fill out the label completely including percentages of constituents the hazards of the waste and contact name If you do not know the hazards of your chemical use the MSDS of the chemical to determine what they are Do not date the container or label Deface or remove any original labels remaining on the container to avoid confusion about the identity of the waste Booklets of twenty adhesive hazardous waste labels are available free at the following locations Biochemistry Stores Location J 014 Health Sciences Building Hours Monday Friday 8 15 12 00 1 00 4 45 Last day of the month 8 15 12 00 1 00 3 30 Chemistry Department Research Stockroom Chemstore Location 036 Bagley Hall Hours Monday Friday 8 30 12 00 1 00 4 30 Closed on UW employee holidays Hazardous waste labels may also be printed out online at http Awww ehs washington edu epowaste hazwastelabel shtm Or email chmwaste u washington edu to request that labels be mailed to you 3 Location Waste must be under the control of the individual s generating the waste The waste should be in a physically
94. odine When a waste mixture contains one or more halogenated organic compounds the total halogenated organic compound concentration is determined by summing the concentration percentages of each halogenated organic compound If a waste mixture contains more than 0 01 HOC the waste is persistent and therefore hazardous For example a waste contains 0 009 carbon tetrachloride 0 012 DDT and 0 020 1 1 1 trichloroethylene The total halogenated organic compounds concentration calculation indicates the mixture is persistent as follows Total HOC Concentration 0 009 0 012 0 020 0 041 b Polycyclic Aromatic Hydrocarbons The following polycyclic aromatic hydrocarbons PAHs are regulated acenapthylene acenapthene anthracene benzo a anthracene benzo b fluoranthene benzo k fluoranthene benzo q h i perylene benzo a pyrene chrysene dibenz a h anthracene indeno 1 2 3 c d pyrene fluoranthene fluorene naphthalene phenanthrene and pyrene When a waste contains one or more of these PAHs determine the total concentration by summing the concentration percentages of each regulated polycyclic aromatic hydrocarbons If the waste contains more than 1 PAHs the waste is persistent and therefore hazardous For example a waste contains 0 08 chrysene and 1 22 3 4 benzo a pyrene The total polycyclic aromatic hydrocarbon concentration calculation demonstrates the mixture is persistent as follows Total PAH Concentration 0 08 1 22
95. onitoring Reeve ete p ed ee ette usd ertet 5 2 3 Possible Over ExpOsute cos ee er on d ere eer E Od ta rte OTN 5 3 4 Medical Ev luations iae de i de eee restituere tpe ueste Peto de eire ee Lp eee epa ede 5 3 B PERSONAL PROTECTIVE EQUIPMENT PPEB sees nennen nennen nennen rennen enne 5 3 1 Eye PrOleCllOn ida eee ertet eamdem qi ed e E O EEE E EERE 5 5 25 App relcod ai dedo eee Pe hae OR eed iude ivi e eie deese ee e EQUI GER Ae eas 5 5 3 isi eese dete e e eei e ad etes 5 6 4 Cue IE 5 7 5 He ring ProteCtorsos a ue o tetti IHE ge e en pte ta p eer E 5 7 SECTION 6 STANDARD OPERATING PROCEDURES eere ee eese enses enses tosta tuse tasse tasto senses suae 6 1 A STANDARD OPERATING PROCEDURES SOPS eese nennen nennen rene enne enne 6 2 B SOPCOMPONENTS certe b teet tee Bois aote Agi etie eei Pee Baebes egt 6 2 Le Required Components s sie oU Be Re e E get e Ded eite eer ilte descent 6 2 27 ADpedtanee iier ER EROR ERU RE PRO ERR EROR ta PEL UR E AFER E ETUR REEF eire 6 3 C EXAMPEEGENERIC SOBS iieri ette i tid pe rere eed entities 6 4 D SOPDEVEEOPMENT iieteteteer e Ete etre ere RE gerente Rede e ERE 6 4 1 Step J Modify Existing SOP Seii eese tee tette bee rapi eee Yee ee Date e Ee OE a teen edad 6 4 2 Step2 Identify Requirements eie eu t IR EP
96. ontainers larger than 5 gallons are not permitted in laboratories without specific approval Table 2 3 Approved Flammable Liquid Storage Containers Container Type Flammable Liquids Combustible Liquids Class I A Class I B Class I C Class Il Class 111 Flash Point Flash Point Flash Point Flash Point Flash Point lt 73 F lt 73 F 273 F 2 100 F gt 140 F Boiling Point Boiling Point and and lt 100 F gt 100 F lt 100 F lt 140 F Ethyl ether Hexane Diesel fuel Mineral spirits Kerosene Glass 0 5 L 1 05 pt 1L 1 05 qt 5 L 1 3 gal 5 L 1 3 gal 20 L 5 3 gal Metal 5 L 1 3 gal 20 L 5 3 gal 20 L 5 3 gal 20 L 5 3 gal 20 L 5 3 gal Rigid Plastic IBCs UN 31H or 31H2 0 0 0 3000 L 3000 L Composite IBCs w flexible inner receptable UN31HZ2 0 0 0 0 0 Polyethylene UN 1H1 5L 1 3gal 201 5 3 20 L 5 3 gal 4501 450L Safety Can 10 L 2 6 gal 20L 5 3 gal 20 L 5 3 gal 20 L 5 3 gal 20 L 5 3 gal Containers may be up to 5 Liters for reagents of Analytical Purity Grade or High Grade UW Environmental Health and Safety 2 12 September 2009 Section 2 Chemical Management Laboratory Safety Manual E CHEMICAL LABELING 1 Original Container The label on an original container must be legible be written in English and include the chemical name the hazard warnings and the manufacturer s name and address
97. or Mercury D 18 Figure D 13 Example SOP for Oxidizer Use D 19 Figure 0 14 Example SOP for Peroxide Forming Chemicals Use D 20 Figure 0 15 Example SOP for Phenol Use D 21 Laboratory Safety Manual Page 2 3 Change 1 July 2010 Section 2 Chemical Management 9 Homeland Security Chemicals of 2 27 a Do Not Ship List inne tete es 2 28 b Do Not Ship 2 28 c Disposing of Chemicals on the Do Not Ship List 2 28 10 Process Safety for Highly Hazardous Chemicals 2 29 Tables Table 2 1 Chemical Use Category Segregation Table 2 8 Table 2 2 Example Hazardous Material Quantity Limits 2 10 Table 2 3 Approved Flammable Liquid Storage Containers 2 11 Table 2 4 Toxic Gas Generators sse 2 17 Table 2 5 _ 7 amp 2 17 Table 2 6 Chemicals that May Polymerize 2 17 Table 2 7 Pyrophoric Chemicals seen 2 18 Table 2 8 Water Reactive 2 18 Table 2 9 Nitrated Compoungds
98. ory Equipment and Facilities 4 Glove Boxes Glove boxes generally operate under either positive or negative pressure to the lab depending on the process or material used Positive pressure glove boxes are used when you are trying to protect your material from contamination Negative pressure glove boxes are used to provide increased operator protection Glove boxes should be thoroughly tested before each use and there should be a method of monitoring the integrity of the system such as a pressure gauge 5 Biological Safety Cabinets Biological Safety Cabinets BSCs are laboratory hoods designed to protect the worker and laboratory from the biohazards infectious agents of the experiment by drawing air across the samples and away from the worker and into a HEPA filter There are two types of BSCs The Class Il type A and Class II type B1 units recirculate filtered air into the laboratory and are not designed for chemical use for this reason The Class II type B2 unit is designed for use of some chemicals but is not a substitute for a fume hood The use of chemicals in this type of hood needs to be carefully evaluated so that the protective barrier HEPA filters is not destroyed by the chemicals Biological Safety Cabinets are certified annually by EH amp S If a BSC fails the certification it may not be used until repaired unless specifically authorized by the Institutional Biosafety Officer BSCs may not be repaired or moved unti
99. ot appear to be clean and empty Examples of equipment that must be decontaminated include centrifuges incubators fume hoods cryostats ovens biosafety cabinets refrigerators freezers sinks storage cabinets lockers bins and tanks Tanks have the potential to be a confined space hazard and thus require special procedures call 206 543 7388 Any equipment capable of generating dangerous radiation or containing radioactive sources must be checked by the EH amp S Radiation Safety Office prior to public sale Please contact the Radiation Safety Office 206 543 6328 These items include e Gas chromatographs e Germicidal UV lamps e Lasers e Sointillation counters e X ray equipment UW Environmental Health and Safety 4 18 Change 1 July 2010 Section 4 Laboratory Equipment and Facilities Laboratory Safety Manual e Any item with a radioactive sticker The following items CANNOT be accepted by Surplus Property Contact the EH amp S Environmental Programs Office at 206 616 5835 for information on how to dispose of these items e Capacitors transformers note some equipment may contain transformers such as x ray equipment and electron microscopes These transformers may be accepted but must be drained of oil and the oil must have been tested and certified by EH amp S as being non PCB oil e Gas cylinders and other pressurized containers vessels e Instruments containing mercury e Equipment containing asbe
100. p others in your workplace know that the container contains hazardous waste not the original chemical Do not recycle glass or plastic containers that contained chemicals unless approved by EH amp S Recycled glass and plastic is used for beverage and food containers so the recycling industry does not accept chemical containers However EH amp S does recycle large plastic and metal drums see http www ehs washington edu eporecycle drums shtm for more details It is illegal to dispose of hazardous waste by leaving non empty containers of chemicals in the fume hood or elsewhere to evaporate the chemical See also the empty container recycling guidelines on the EH amp S website at http Awww ehs washington edu epowaste trashcontainer shtm 3 Trash Disposal of Contaminated Items Used gloves and other commonly used items besides empty containers can be placed in the trash if they are not grossly contaminated with hazardous chemicals If you have an item that is grossly contaminated dispose of it as hazardous chemical waste Examples of grossly contaminated items include used spill clean up materials items such as gloves and equipment contaminated from a spill and used equipment that contains hazardous chemical residue Finally EH amp S encourages you to collect items that look like they might be contaminated by chemicals such as weighing papers and gloves in bags and then label the bags non hazardous waste befo
101. persensitivity Reaction eese eese sees esee eene entente entente tenete EE tenente nee G 4 3 Immediate Hypersensitivity Reaction G 4 D GLOVE SIZES iden eror PORTER RE EE EET TRI PER OTRO ER CHAR ert G 5 E OFF ECAMPUS SOURCES POR GLOVES ettet territi cete ri PER Tenue RERO e EE ee ee tide atecboines G 5 F GLOVE SELECTION FOR SPECIFIC CHEMICALS CHART esee G 6 APPENDIX PARTICULARLY HAZARDOUS SUBSTANCES H 1 As HAZARDOUS CHEMICALS rone EIE X YR ERREUR RE NOTE ERE re YR REESE SES mp e e beu ERE E ee ENESES H 1 B PARTICULARLY HAZARDOUS SUBSTANCES ise enne H 1 Il Highly TOXIC iue EGRE RES ERRARE DUREE ENGEL H 2 2 Highly Dangerous su s ie ee E I RU RR NE oe ei e OR ee Nee cen peut H 2 J Select Carcinogensoa oua depot p t ep lesen eiua quen eire pa A ave ise H 2 4 Reproductive Toxicants i otii d de ec eee ete dede fee roe ertt bee der H 3 Laboratory Safety Manual 3i Select Toxins Tables Table 2 1 Table 2 2 Table 2 3 Table 2 4 Table 2 5 Table 2 6 Table 2 7 Table 2 8 Table 2 9 Table 2 10 Table 2 11 Table 2 12 Table 2 13 Table 3 1 Table 4 1 Table 4 2 Table 5 1 Table 5 2 Table 5 3 Table 9 1 Table 9 2 Table 9 3 Table 9 4 Table 9 5 Table B 1 Table G 1 Table G 2 Table G 3 Table H 1 Table H 2
102. pervisor Occupational Health and Safety Office John Eriksen Training Manager Department Administration and Finance Office Katia Harb MS Compliance Analyst Research and Biological Safety Office Hal Merrill EH amp S Technologist Building and Fire Safety Office Megan Kogut PhD Health and Safety Supervisor Environmental Programs Office Page i Laboratory Safety Manual September 2009 Table of Contents Environmental Health and Safety 44429 UNIVERSITY of WASHINGTON Contents SECTION 1 CHEMICAL HYGIENE PLAN RESPONSIBILITIES e eeeeee eese 1 1 A IPURPOSE sist ahaha celta ieee DP DURO DD Deep teer 1 1 p Chemical Hygiene Plan CHP i nte esce RE mete t ree ee e nS 1 1 2 Regulations Pertaining to the Chemical Hygiene 1 1 2 3 Chemical Hygiene Plan Accessibility 1 3 4 Other Plans did References eee e e m ca Ri ea ed a ete 1 3 2 Applic bilityto Studeni ci END PHENOM Seneca 1 3 B SCOPE AND APPLEICATION tt ete tete irte geh Ert te peste eR a el tees sues 1 3 i Chemical Laboratory 35st eddie pee de aee dee e Ue ege eed etes 1 4 2 Chemical and Non Chemical Hazardis eese eene tenen nennen een rennen tenete 1 4 C s RESPONSIBILITIES ode bete eoe e e ea deret p eee eee 1 4 1 Laboratory Chemical Hygiene Officer CHO eese e
103. pug 3 12 J Scintillation Fluids nee em dete edet i ect Pei des 3 12 60 Dilution Prohibition ien RR e P Re 3 12 G CHEMICAL WASTES OF PARTICULAR CONCERN esee nee en rennen 3 13 12 Unknown Chemicals ene e e T UR m a ce ET m deine sh bee iere Ue ov tote trey 3 13 2 Potentially Explosive eese tte letus ie eee erectae beoe ned 3 13 3 Legacy CHEMICALS eoe o oe m DERE E te ee reati edes reete 3 14 H HAZARDOUS WASTE MINIMIZATION eerte netten nennt nennen teen nne a ten enne nene 3 14 1 Chemical Procurement and Chemical Exchange eese eene eene 3 14 2 Treatment and Recycling in the Laboratory essent 3 14 3 Hazardous Materials Recycling eese esee eene nennen een eene entente tenerent trennen 3 15 I SOLID WASTE AND REGY CIUING deti dose pep de bei e one eite e p edges 3 15 k Paper nd Cardbo rd eo td e Qe EE RE e E e bs reportes 3 15 2 OPlasticand Glass eeepc C P Oe EQ eti eri e OP a EEA EEEa 3 15 32 Packaging Materials e ERR 3 15 4 Media and Printer Cartridgesz i Ra PE Uo RUE 3 15 SEMEL E P L betes 3 15 J JSHARPSAND EAB GLASS iste rete ete eed Ha Ra ee His E ETN 3 16 1 ATUM ERR 3 16 2 Eab Glass Broken Glass 3 it rt tet Hotes et ete etes dietis 3 16 K INFECTIOUS OR BIOLOGICAL WASTE eene
104. re you place them in the trash Custodial staff members are sometimes understandably nervous when handling laboratory trash a white residue or a few drops of water in the trash could be a dangerous chemical Taking an extra step to bag these items can be a nice gesture Custodians may refuse to collect trash that appears to contain hazardous items If they refuse to collect trash they will leave a Notice of Improper Waste Disposal Practices form UoW 1970 Once corrections are made they will collect the trash F SEWER DISPOSAL All wastewater discharged to the sanitary sewer system must be under the local Sewer Discharge Limits designed to protect surface waters and maintain the quality of biosolids from wastewater treatment plants 1 King County Local Sewer Discharge Limits In King County you may dispose of some chemicals down the sanitary sewer drain in some circumstances This method of disposal is also known as sewering Records of this disposal must be kept as described in Section F 3 below If your waste qualifies as hazardous waste according to the criteria in Section B above then you may not sewer the waste King County has also published local discharge limits for commonly used chemicals These limits are on the EH amp S website at http www ehs washington edu epowaste sink shtm They apply only to UW Seattle UW Bothell and other sites within King County UW Environmental Health and Safety 3 12 Change 1
105. rgyria asphyxia asphyxiant asthma ASTM asymptomatic ataxia atrophy autoignition temperature bases boiling point bradycardia bronchitis buffer CAA C or ceiling carcinogen CAS number Laboratory Safety Manual American National Standards Institute A privately funded voluntary membership organization that identifies industrial and public needs for national consensus standards and coordinates development of such standards Many ANSI standards relate to safe design performance of equipment such as safety shoes eyeglasses smoke detectors fire pumps and household appliances and safe practices of procedures such as noise measurement testing of fire extinguishers and flame arresters industrial lighting practices use of abrasive wheels etc ANSI 1819 L Street NW Suite 600 Washington DC 20036 202 293 8020 http www ansi org A water based solution The adverse effects to marine life that result from being exposed to a toxic substance Local or generalized impregnation gray blue color of the body tissues with silver Lack of oxygen and thus interference with the oxygenation of the blood Can lead to unconsciousness A vapor or gas that can cause unconsciousness or death by suffocation lack of oxygen Most simple asphyxiants are harmful to the body only when they become so concentrated that they reduce oxygen in the air normally about 21 to dangerous levels 1896 or lower Asphyxiation is one o
106. riment as long as it meets sewer disposal limits There are two reasons why you may not dilute to meet the limits First if everyone were allowed to do it the practice would use a lot of water Secondly many toxic chemicals such as metals and organic compounds partition into organic matter At the wastewater treatment plant these chemicals would end up in the biosolids no matter how dilute they are The biosolids can be re introduced into the general environment such as in King County where it is UW Environmental Health and Safety 3 13 Change 1 July 2010 Laboratory Safety Manual Section 3 Chemical Waste Management sold as fertilizer for tree crops and for landscaping Therefore it is environmentally preferable to manage concentrated wastes as hazardous waste rather than dilute to meet the discharge limit For more information see the EH amp S website on sewer disposal at http Awww ehs washington edu epowaste sink shtm G CHEMICAL WASTES OF PARTICULAR CONCERN 1 Unknown Chemicals Without an accurate chemical name and concentration range unknown or unidentified chemicals cannot be safely handled or disposed of The best way to prevent unknowns is to label all chemical containers and make sure that the labels stay in good condition over time If you have an unknown chemical keep it where it is or store it temporarily in the fume hood whichever you believe to be safer Find out as much information as you can a
107. s Blank logs are available at http www ehs washington edu forms epo chemlog pdf 4 Soaps Bleach and Acetone When you are washing glassware or equipment you will likely use chemicals such as detergents and bleach Standard household bleach and other cleansers may go down the drain Acetone may not go down the sink at any concentration If you use acetone to rinse off items you must collect any excess acetone in a securely capped properly labeled waste container and dispose of it as hazardous waste see the hazardous chemical waste page for more information You may not store acetone squeeze bottles near the sink Do not use chromate based cleansers There are many less toxic and non carcinogenic alternative cleansers that work just as well 5 Scintillation Fluids There are only three liquid scintillation cocktail products currently approved by the State of Washington Department of Ecology for disposal down the sanitary sewer They are soluble or readily dispersible in water and contain less than 1096 non ionic surfactants Other scintillation fluids may claim to be safer but because they contain high concentrations of flammable surfactants they are not approved for sewer disposal 6 Dilution Prohibition It is illegal to dilute your chemical waste solely to meet sewer discharge limits However you may sewer wastes such as equipment rinse water or any chemical treatment that you do as a normal part of cleaning up after an expe
108. s too small for labels installed into a process or would become unusable for its intended purpose if labeled must still have its contents identified in some way Use any labeling method that enables employees and visitors from other agencies such as the fire department to identify the chemicals and their hazards Examples include a sign identifying the materials and their hazards or color or numeric codes cross referenced on a chart or room diagrams identifying locations of the chemicals and hazards Label chemicals that form peroxides or other hazardous products when exposed to air with the date the container was first opened using the form UoW 1716 shown in Section G 2 b below Label chemicals listed in the Chemical Facility Anti Terrorism Standards CFATS with warning labels as described in Section G 9 b below to remind workers that the substances are regulated and cannot be shipped off campus without prior EH amp S notification Waste containers must be labeled following guidelines in this manual in Section 3 for hazardous chemical waste For radioactive waste see Section 14 of the UW Radiation Safety Manual For biological waste see page IV 42 of the UW Biohazard Manual If re using a container to hold waste the container must be compatible and appropriate for the waste Completely deface all old labels a UW Hazard Label The UW Hazard Label Figure 2 1 is no longer available from University Stores but previous stocks can continu
109. ssium magnesium etc Segregate oxidizing acids from organic acids such as glacial acetic acid and from flammable and combustible materials such as cardboard boxes Segregate acids from chemicals which could generate toxic or flammable gases upon contact such as sodium cyanide iron sulfide calcium carbide etc Segregate acids from bases Bases Segregate bases from acids metals explosives organic peroxides and easily ignitable materials Flammables Store in approved safety cans or cabinets Segregate from oxidizing acids and oxidizers Keep away from any source of ignition heat sparks or open flames Also see section D 3 below Store in a cool dry place Keep away from combustible and flammable materials Keep away from reducing agents such as zinc alkali metals and formic acid Segregate from acids and oxidizers Water Reactive Store in a cool dry place away from any water source Have a Class D fire extinguisher Chemicals available in case of fire Also see section G 1 e below Pyrophoric Materials that will react with the air to ignite when exposed e g tert butyl lithium Substances Store in a cool dry place making provisions for an airtight seal Also see section G 1 d below Light Sensitive Store in amber bottles in a cool dry dark place Chemicals UW Environmental Health and Safety 2 9 September 2009 Laboratory Safety Manual Section 2 Chemical Managemen
110. stos including but not limited to autoclaves laboratory ovens fireproof file cabinets anything that produces high heat The type of decontamination will vary depending on the hazardous material and the type of equipment Note that personal protective equipment should be used when decontaminating equipment Below are some requirements and guidelines for decontamination as well as contact information for questions 1 Equipment Used to Process Store Chemicals Safely remove or drain chemicals from the equipment including any oil or coolant Collect the chemical s for reuse or dispose of as hazardous waste If applicable use an inert gas or liquid to purge or rinse out chemical residues In some cases rinseate will need to be disposed of as hazardous waste as well See our website at www ehs washington edu epowaste or call the EH amp S Environmental Programs Office at 206 616 5835 for questions regarding hazardous waste disposal of chemicals and or rinseate Decontaminate the equipment as necessary For example use solvents to remove viscous or non water soluble contaminants Then scrub decontaminated equipment thoroughly with warm soapy water Rinse and dry Wash and or rinse water and solvents may need to be managed as hazardous waste Contact the EH amp S Occupational Health amp Safety Office at 206 543 7388 for more specific information about decontamination 2 Equipment Used to Process Store Radionuclides Conduct a thorough radiat
111. substances highly dangerous or toxic chemicals select carcinogens mutagens and teratogens should be stored together if compatible Signs should be posted indicating their location and unique hazards 7 High Degree of Toxicity Chemicals with a high degree of toxicity e g venoms mycotoxins and select agents should be doubly contained and stored in a locked area accessible only by authorized personnel Use containers that are chemically resistant and non breakable 8 Chemical Waste Store chemical wastes following the same guidelines as above Original container labels must be obliterated and the containers must be labeled with a completed University of Washington hazardous waste label Secondary containment is required if chemical waste is stored near a floor drain or other drain to sanitary sewer Avoid mixing incompatible waste materials Serious laboratory accidents such as a death at the University of Washington in the early 1970 s have occurred when people have mixed incompatible waste materials For more information about chemical waste see Section 3 of this manual b Incorrect Storage Practices 1 Acids Do not store inorganic acids with flammable solvents flammable acids or combustibles such as cardboard Contact of a concentrated oxidizing acid with a flammable solvent may result in a fire or an explosion Other incompatible chemical storage practices are shown above in section D 1 UW Environmental Health and Safet
112. t Peroxidizable Store in airtight containers in a dark and cool place Most peroxidizable compounds Chemicals are flammable and should be stored in a flammable liquid storage cabinet or room Label containers with receiving and opening dates Periodically test for the presence of peroxides Discard before exceeding expiration date Also see section G 2 b below Toxic Chemicals Store according to the nature of the chemical using appropriate security where necessary Also see section G 1 a below Nitrated Nitrated compounds can be considered explosive special care and handling may be compounds required Also see section G 2 a below 2 General Chemical Storage Guidelines Follow good storage practices no matter wherever the chemicals are stored i e cabinets refrigerators or shelves a Good Storage Practices 1 Cabinets Whenever practical chemicals should be stored in approved cabinets 2 Shelves All shelves should be securely anchored to walls and fitted with 2 inch lipped edges or enclosed in cabinets with latched doors 3 Heavy Objects Heavy objects should be stored on lower shelves 4 Corrosives Corrosives should be stored only below eye level 5 Secondary Containment When practical chemicals in the same hazard class that are compatible should be stored in secondary containment tubs that are chemically resistant and unbreakable 6 Consistent Chemical Storage Locations Particularly hazardous
113. te a xc tee ee d teda tt edi a a ec ete nee eee eae e de i eb de 7 4 6 Emergency Response e et e i o Desde Pe ERE toe EEE E Ea R 7 4 C CLASSES iod ebeedeotteeendDeepeeie en Ed ueni PROIBItei pee ere 7 4 d xReguired Training usse ette nbsp erm eet peret 7 5 2 Recommended Training iie see tt etn Ub RE aero t eR i E cei Dd 7 5 D SAFETY TRAINING RECORDS entente 7 6 SECTION 8 RECORD KEEPING 8 1 A CURRENT RECORDS MAINTAINED IN 8 1 1 Laboratory Safety Manual Chemical Hygiene Plan eee nennen 8 1 22 Chemical Inventory ausi e n UR RR REI GI ERI REPE rH FEED rete 6 2 3 Material Safety Data Sheets MSDSs essent enne 6 2 4d Uncident Accident Reports escasa e voee elo re reb enr E pee aer eae ER 6 2 3 Safety Training Records iioi ee ette ee e EFE Eee Eee 6 2 6 Shipping Papers Bills of Lading essent eene nennen tne nennen trennen rennen nenne 6 2 75 XewerDischartgeLogs sees sg UR US PUERO qe d pe eS 6 2 6 Exposure Monitoring is eet esce i ees le ee E io 6 2 B OBSOLETE AND SUPERSEDED RECORDS FROM THE LABORATORY em 8 3 1l Obso
114. te Flow Charts which are located online at http www ehs washington edu rbsresplan sharp shtm flowcharts 2 Lab Glass Broken Glass Laboratory glass including plasticware is any item that could puncture regular waste bags and therefore endanger waste handlers Laboratory glass must be placed in a sturdy cardboard box lined with plastic for safety during transport through the building Any cardboard box may be used provided it is sturdy does not have holes in the bottom or sides and of a size that will not weight more than 40 pounds when full Boxes must be labeled with the room number and principal investigator s name and should be sealed with tape identifying the box as containing laboratory glass Boxes and tape are available in the Chemistry stockroom and from suppliers and tape is also available from Biochemistry stores If the printed tape is not available the box can be sealed with other packaging tape as long as the box is well marked as containing laboratory glass The sealed box is placed alongside the regular waste container for collection by Custodial Services Never use these boxes for the disposal of sharps biohazardous materials that have not been autoclaved liquid wastes chemically contaminated laboratory glassware plasticware or chemical containers that cannot be disposed of as regular solid waste Laboratory glass that is disposed of in cardboard boxes must be clean or appropriately decontaminat
115. te reu ee Seo EPI tre pe e 9 14 4 Radioactive Material Spills edere a HR e P peo ee eret 9 16 2 Farthquake RESPONSE eI pe RE ds 9 18 0 Gas Leaks or Other Od OPS ie iso pede aed ede et i ae b ie ep iesus 9 18 7 dU lity Outdge eiii RU A UATE Lee Ba 9 19 Laboratory Floods ideae ote e PERI ERROR RR RE eris 9 20 9 Jnclement Weathler riren en Et I Pt ee PERSE RD E dE 9 21 10 Intruders Suspicious Packages and Demonstrators eese rene 9 21 11 Emergencies during Field 9 22 SECTION 10 MOVING IN MOVING 10 1 A MOVING IN OCCUPYING A NEW OR REMODELED LABORATORY ere 10 1 1 Before the Moves si eager edi tide eee eite Ee dre epe edet 10 1 2 After th MOV ere sd vier eine ren tre dhs dened PER ER ERES in e ir o dieere 10 2 3 Checklist for Moving Into a Laboratory esee ene eene tente nene trennen 10 3 B MOVING OUT VACATING A LABORATORY eese nennen een eene treten trennen trennen 10 3 Responsibilities ite edere rS GL Eie YI eet ee eerte e deer and 10 3 2 Transportation Requirements and Logistics eese eese neret enne nennen trennen 10 4 3 Checklist for Laboratory Moveouts ecciesie steer ete ier der tiene ibo tenete epos 10 5
116. the pharmacy formulary list go to https eres lib washington edu coursepage asp cid 1805 amp page 01 If you do not know exactly what is needed email questions to drugsvcs uw edu 3 DEA Controlled Substances DEA registrants can obtain controlled substances from a drug company wholesaler or UWMC Drug Services If you wish to order a controlled substance through Drug Services a current Controlled Substances Registration Certificate must be faxed or mailed to Drug Services before an order can be filled Controlled substances must be stored in a locked cabinet with limited access A perpetual inventory must be maintained and the inventory forms used must meet DEA and State regulations Expired or waste undesired drug must be kept secure in a locked cabinet in a separate container properly labeled for content and inventoried until disposal Drug Services or EH amp S will provide the contact information of DEA licensed reverse distributors who must be used for disposal For more information contact Drug Services drugsvcs 2uw edu or EH amp S ehsdept uw edu or 206 616 5835 4 Non Denatured Ethyl Alcohol Instructions for obtaining approval and purchasing non denatured ethyl alcohol are detailed on the UW eProcurement web site at http www washington edu admin stores eprocurement Instructions for maintaining accountability for tax free ethyl alcohol are in Administrative Policy Statement APS 15 1 at http Avww washington edu admin ru
117. to Additional Containers leet tete trio tea ra nei bed pe teres 2 12 3 FPabelingInstFuCtiOns susce tette te e Rr d eei eei e en 2 12 TRANSPORTING CHEMICALS pee irr etri e EUR cove ch 2 14 1 Transporting between Floors and Buildings on Campus eese eene 2 14 2 Transporting Chemicals off Campus eese eene teen ener enne tenete trennen trennen nes 2 15 Gr SPECIAL CHEMICAL HAZARDS sc tio ioter tette die cere bet esee m Remi E 2 16 Lh Reactive Chemicals iid e TR ER NI re Nee d edi e eire Pee ope ies ee a Bk 2 16 2 Potentially Explosive Chemicals ener eene nnne tene tenete nennen 2 18 3 Highly Toxic Substances ies e eee e EHE ESIOS petet ien 2 21 4 Carcinogens and Reproductive Hazards eese eene eee eene enne nenne 2 22 3 sensitizing or Allergenic Chemicals se e teet e e e e o RR 2 22 Synthesized Chemicals aie nee etes pi e ES Be ee Eee o REOR CUR MEI Rte 2 23 7 Compressed Gases and Gas Cylinders eese eene rennen tne tenete trennen 2 23 6 Flammable and Combustible Liquids eese eee eene nnne een een eerte nnne 2 25 9 Homeland Security Chemicals of rene eene ene 2 27 10 Process Safety for Highly Hazardous Chemicals eene 2 29 SECTION 3 CHEMICAL WASTE MANAGEMENT eres enean tna tn statu atn tatu sons t
118. y 2 10 Change 1 July 2010 Section 2 Chemical Management Laboratory Safety Manual 2 MHeat Direct Sunlight Exposure of chemicals to heat or direct sunlight should be avoided Even if the chemical is stable plastic containers have degraded from sunlight 3 Storage on Floors on Bench Tops or in Fume Hoods Chemicals should not be stored on the floor or be so numerous as to clutter bench top work areas Storing more than a few chemicals in a fume hood will compromise the effectiveness of the hood unless they are stored on a shelf a few inches above the work surface of the fume hood so that air can enter the slot at the back of the work surface 4 Storage Height Do not store heavy containers on the floor or above waist level Do not store corrosives above eye level Do not store items closer than 18 inches from the ceiling if the area has fire sprinklers 5 Hallway Storage Do not store chemicals in hallways corridors and exit ways 3 Chemical Storage Quantity Limits a Control Zones Chemical quantities in most University buildings are limited by the local fire code which is based on the most recent International Fire Code IFC adopted by the local jurisdictions Note Local amendments to IFC have been made by Bothell Seattle and Tacoma Fire Departments Limits by hazardous material classification apply to a control zone that may include up to an entire floor of a building Quantity limits may be
119. y checking that connections are tight Clean up spills as Soon as possible and minimize clutter to avoid inadvertent spills Prevent inhalation of chemicals For example use a fume hood whenever handling volatile or aerosolized chemicals even if they are of relatively low toxicity Cap chemicals as soon as is convenient Limit the smelling of chemicals to the minimum amount necessary and only if no other method of identifying a chemical is available Prevent ingestion of chemicals For example do not taste chemicals Mouth suction must not be used to pipet chemicals or to start a siphon instead a pipet bulb or an aspirator must be used to provide a vacuum UW Environmental Health and Safety 2 4 September 2009 Section 2 Chemical Management Laboratory Safety Manual Prevent injection of chemicals For example cap needles as soon as the injection is complete Use needles with inherent safety devices that prevent inadvertent needle sticks Dispose of sharps into appropriate waste containers If operating a high pressure system never check for a pressure leak using your hands 3 Washing Hands Wash hands well with soap and water after removing gloves and before leaving the laboratory area Never wash with organic solvents See Section 5 B Personal Protective Equipment and Appendix G Gloves for more information 4 Food and Drink Food and drink increase the chance of exposure to chemicals and are prohibited from being

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