User`s Manual
Contents
1. horizontally or upside down when there is liquid inside the tip otherwise the liquid will get inside the main body which may cause deterioration of the instrument If liquid is sucked inside the main body inside the nozzle clean as quickly as possible Liquid inside the nozzle is a main cause of precision reproducibility loss and may lead to breakdown of the instrument When turning the push button do not exceed the specified sample volume limit as this may damage the instrument or cause deterioration Do not perform pipetting with less liquid than the set volume If the quantity is less than the set volume this may cause the liquid to scatter into the main body which may result in deterioration of the instrument The pipette can be used between 4 C and 40 C but the specifications may vary 10 Depending on the frequency of use the pipette should be cleaned in a soap solution and maintained the airtight chamber according to this manual 3 Contents e Operating procedure vini 5 e Disassembling Reassembling the airtight chamber 9 e Specifications een 10 e Troubleshooting sini iii 11 Parts I nee 12 V Operation 1 Volume setting For each volume the graduation consists of three numerical values that are read from top to bottom Turn the push button to the left or right aligning it with the numerical value of the setting volume Graduation units of each model are as shown in the chart below Fig A V2. Light amp Ergonomic Pipette NICHIRYO Digital micro pipette for liquid handling User s Manual In Vitro Medical Diagnostic Devices 98 79 EC Annex Ill self declared ISO 8655 STANDARD CERTIFIED ISO9001 Always Pursuing Originality Since 1944 NICHIRYO Light amp Ergonomic Pipette NICHIRYO e Thank you for purchasing the NICHIRYO Le e Before proceeding to work with the NICHIRYO Le please carefully read this manual for proper use of it l Introduction This operator s manual explains the operation of the Nichiryo Digital Micro Pipette NICHIRYO Le Please read carefully to ensure safe and correct operation of the instrument Please pay special attention to passages marked in bold lettering Be sure to read cautions that need strict observance and underlined sections before using the instrument II Features e As volume amount setting is done digitally easy volume setting is possible with a simple press of the push button e Through adoption of an easy to grasp design and a lightweight construction in comparison with our Nichipet EX model we have realized a product design that allows long hours of use without operator fatigue e The structure of the unit has been designed to reduce the effects of hand temperature on performance accuracy e Our lineup of 5 different models allows a wide range of sampling between 1 0ul 1000ul III Standard Accessories e Tips x 3 e Grease x 1 e User s Manual W
3. at it meets published calibration specifications The precision and accuracy results obtained for this pipette are provided on the enclosed calibration certificate Because temperature and humidity conditions affect the calibration results of liquid measurement devices your pipette should be calibrated under conditions of use The calibration results obtained in your laboratory may vary from our results due to differences in environmental testing conditions For repair service or information you may contact your local distributor Manufacturer ONICHIRYO CO LTD Tokyo office 1 10 1 Kandanishiki cho Chiyoda ku Tokyo 101 0054 Japan TEL 81 3 6273 7652 English FAX 81 3 6273 7944 URL http www nichiryo co jp E mail i sales nichiryo co jp 2013 Ver 1
4. e recommended solutions please stop using the instrument immediately and request service At that time please be sure to make sure that the instrument is not contaminated with any substances such as microbes or chemicals that are harmful to the body Grease the plunger and O ring 11 H Replacement parts list Service parts list Description NIE 10 NLE 20 NLE 100 NLE 200 NLE 1000 First spring holder NPE 020A NPE 020A NPE 020A NPE 020A NPE 020B Stroke ring NPE 018A Second spring NPE 023A NPE 023A NPE 023A NPE 023A NPE 023B NPE 021A NPE 022A NPE 018A NPE 021B NPE 022B NPE 018A NPE 021C NPE 022C NPE 018A NPE 021D NPE 022D NPE 018B NPE 021E NPE 022E N3 0711A N3 0711A N3 0711A N3 0711A N3 0711A 0 O ring Grease As the above replacement parts are specifically designed for each volume size please indicate the volume size of your instrument when placing your order H Nichiryo Genuine Tips e Tips Autoclavable ee are e i e Racked tips Autoclavable Volume SE 960 tips Ei m Nee 96 tips x 10 cases yo ki 960 tips BMT2 SGR 2 200 NLE 20 100 200 96 tips x 10 cases a A 960 tips BMT2 LGR 100 1000 NLE 1000 96 tips x 10 cases 12 Memo Memo Inspection and Calibration Statement The enclosed pipette was tested and calibrated under closely controlled environmental conditions to ensure th
5. hen unpacking package check to make sure that the above mentioned items are included IV Safety Precautions e Please be sure to carefully read the sections titled Safety Precautions and AN Cautions before using the instrument e The contents of CAUTIONS are matters that require the user s attention not only for using the Nichiryo Le properly but for preventing the user from accidents and physical damage eAfter reading this manual please keep it in a convenient place for easy referral at any time Please read the following prior to use for your safety and correct usage of the instrument CAUTIONS Be sure to observe the following instructions for using the NICHIRYO Le properly and safely Do not use this instrument for any purpose except pipetting liquid Do not modify this instrument as modification may cause an accident Do not use this instrument for pipetting any liquid to be injected into the human body Do not eject the tip towards anybody Do not discharge any sample liquid while pointing the instrument at anybody Some liquids are dangerous and may cause bodily harm Do not eject the tip while liquid is still inside Handle the tip with care as the point is sharp and dangerous Firmly attach the tip to the nozzle If not attached securely it may fall off causing the sample liquid to scatter When handling liquid harmful to the human body be careful not to directly touch the tip during or after u
6. iquid Fig B 6 Wipe any droplets away from the outside of the tip using a medical wipe and avoid touching the tip s orifice Note Do not aspirate when push button position is at c Note The push button has to be pushed and released slowly Otherwise it may cause wrong accuracy UUU MT ot l e u Ejector button 0000 Gud J Fig B 3 Dispensing liquid 1 Gently place the end of tip against inside wall of recipient vessel just above liquid surface 10 degrees to 45 degrees in angle 2 Press down the push button slowly and smoothly to the first stop b Wait for a few seconds then press down the push button to the second stop to expel the last drop of the liquid from the tip Fig D 3 Release the push button slowly 4 Press the ejector button to remove the tip and dispose of it Fig D A Caution When using any toxic or harmful liquid to human do not touch any used tips Fig C Reference 1 Pre rinsing When attaching a new tip before sampling repeat suction and discharge of liquid to the first stop position 2 to 3 times Pre ri
7. nsing will result in a high degree of accuracy In addition especially when strict accuracy is sought we recommend this method when sampling various types of liquid 2 Sampling high density liquids and viscous solutions When aspirating once the liquid has entered the tip wait 2 to 3 seconds before removing the tip from the liquid When dispensing wait 2 to 3 seconds at the first stop position before pushing to the second stop position 4 Plunger 5 First spring 6 First spring holder 7 Stroke ring 8 Second spring 9 O ring retainer 10 O ring VI Disassembly and assembly of airtight section For airtight section maintenance or when a problem such as that clearly stated in the Trouble Shooting section of this manual occurs carry out disassembly inspection and cleaning following the instructions below 1 1 2 3 Disassembly Remove the ejector pipe With the ejector button depressed slide the ejector pipe to the right clockwise direction and remove from the ejector shaft Next pull out the ejector pipe Refer to Fig D Remove the bottom nut While holding down the nozzle cylinder turn the bottom nut counter clockwise and remove from the body At this time the spring inside the nozzle cylinder may cause the internal parts to pop out Please use care and remove slowly Remove each part Remove the parts in the nozzle cylinder First spring plunger first spring holder second sp
8. olume graduation unit chart NLE 10 NLE 20 NLE 100 NLE 200 NLE 1000 od OJ po OJ O Laj 6 9 LINL LOA LIA LIL n 9 54 l 16 5 u 95u l 165 u 950 u L 0 95m Observe the following cautions when setting volume 1 When setting to an increased volume First exceed the desired setting graduation by about 1 3 revolution then adjust the indication to the desired graduation 2 When setting to a decreased volume Slowly turn to the precise point being careful not to go past the desired graduation setting A Caution e Do not exceed the stipulated volume range or change the volume capacity This could damage the instrument or impair quality Never turn the push button more than 1 3 revolution past the maximum stipulated volume 2 Aspirating liquid Forward method 1 Attach a new tip to the nozzle end We recommend that you attach it from rack Note It would be recommended that tips are directly picked up from rack And do not twist pipette when fixing tip 2 Press down push button to first stop position b Fig A Don t aspirate the liquid with the push button pressed at the second stop c 3 Hold the pipette vertically and immerse the tip 2mm to 3mm below the surface of the liquid Fig B D 4 Release the push button slowly and smoothly to aspirate the set volume of the liquid Fig B 5 Wait 1 to 2 seconds then withdraw the pipette vertically and carefully from the surface of the l
9. ominal volume and the minimum or 10 of nominal volume Table 1 NLE Maximum Permissible Errors Volume Measured Accuracy Precision Code range uL volume uL at aay NLE 1000 100 1000 Accuracy and reproducibility of 1uL on the NLE 10 model and 2uL on the NLE 20 model will be greatly influenced by the operator s sampling skill The data given in the table conform to the ISO 8655 2 standard 10 VIII Trouble Shooting Inside of nozzle cylinder point is Clean or replace the nozzle clogged foreign matter buildup cylinder part Liquid is not suctioned f The nozzle cylinder is loose Retighten the bottom nut The O ring is worn Replace the O ring Airtight section lubricant grease El aa grease Grease the airtight section has been used up Nozzle cylinder point is worn Suctioned liquid leaks visual inspection shows level Replace the nozzle cylinder from the tip difference Plunger surface is scratched or Renace RR damaged P panas The tip being used does not fit the nozzle Plunger and O ring lubricant grease has been used up Push button operation Liquid has been drawn into the unit Disassemble the lower part and IS poor inside of nozzle cylinder clean Plunger surface is dirty or foreign Disassemble the lower part and matter has adhered to it clean or replace the plunger If you experience any problems other than those noted above or if there is still a problem even after carrying out th
10. ring and O ring The plunger and first spring holder are a complete set on the 1000ul model Once removed carry out part replacement inspection and cleaning of the airtight section A Caution 2 1 2 3 e During disassembling take care not to lose any of the parts Assembly Basically assembly is done in the reverse order of disassembly Referto Fig E Set the O ring on the O ring holder With the plunger tip facing upward attach each part in the order of first spring first spring holder stroke pipe second spring O ring retainer O ring Next insert in the nozzle cylinder Install the ejector pipe Install in the reverse order of disassembly A Caution e After assembly before using the unit test operate it a few times to confirm that there are no problems VII Specifications Nichiryo Le model No NLE is a high quality pipette The technical figures given in the Table 1 NLE Maximum Permissible Errors were obtained using genuine Nichiryo BMT tips Nichiryo declares that pipettes comply with the requirements of the ISO 8655 Standard by type testing The adjustment is carried out under strictly defined and monitored conditions ISO 8655 6 e The basis of adjustment EX e Reference temperature 20 C e Relative humidity 50 e Barometric pressure 101KPa e Use of distilled water grade 3 ISO 3696 e Ten measurements for each test volume at three points nominal volume 50 of n
11. se 10 If the instrument is soiled with liquid harmful to the human body immediately 11 12 take proper measures to clean it safely before proceeding with work Do not use this instrument for stirring liquid or for any other such action Additionally do not use the instrument in any manner that will cause the tip to loosen fall off or the instrument itself to be soiled with scattered liquid Do not use this instrument for any purpose except pipetting liquid Q Please review before using Users are required to strictly observe the following points in order for the instrument to retain its precision and original performance for a long time 1 Do not expose this instrument directly to the sun when working with it or for 2 hours before starting work otherwise the instrument may fail in precise pipetting Avoid working with this instrument in a humid and hot place Just before starting work with this instrument avoid touching the tip and nozzle cylinder as far as circumstances permit If the nozzle cylinder is warmed by your hand it is hard to maintain accuracy For pipetting follow the forward method explained in this manual If it is performed in a different way it may result in inaccurate pipetting Operate the push button slowly If quickly released sample liquid will be sucked into the main body which may result in not only inaccurate pipetting but also deterioration of the instrument Do not hold the instrument
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