- User Manual
Contents
1. PFS210 6 101950 PFS320 PFS290 PFS220 7 PFS230 4 PFS990 PFS980 PFS240 1 PFS250 PFS260 PFS270 PFS280 PFS300 nao P PFS960 PFS970 PFT050 GV3320 LV7070 T l 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PURIFL s Av Item Fraction collector Positioning guide for racks Fraction collector motor X axis cable Fraction collector motor Y axis cable X axis belt Y axis belt Pulleys 1 motor 1 receptor Collector opto sensor X axis Collector opto sensor Y axis ON OFF switch with EMI filter AC Power supply 230 24V 50W Pump Collector fuses 2 5A 110V x 2 Pump Collector fuses 1 6A 220V x 2 Black POM for column holder fixed x 2 screws Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com 33 4 70 03 73 01 Part Number PFS400 PFS410 PFS420 PFS430 PFS440 PFS450 PFS460 PFS470 PFS330 PFS480 PFS990 PFS980 102960 N interfine interchim fr User Manual Item Part Number N Column holder TEFZEL ferrule with Stainless steel lock ring for 1 8 tubing GV1690 PEEK 1 4 28 long nut for 1 8 tubing for GV1690 GV1710 Adapter Luer Lock Male to 1 4 28 Male PEEK PFS770 Guidance part for large black POM holder FO4400 Stainless steel union for flash configuration FV6560 Large black POM injection holder grub2. 100cm 56cm spring 50cm li E v Fill the loop with the content of the syringe If the quantity injected is greater than the capacity of the loop the surplus flows in the waste tube port n 6 In the panel Settings of the edited method select v Equilibration in the section Injection Mode According to the type of the column a time and a flow rate are selected by default They depend on the column parameters but you can modifiy them if needed v Loop injection for the injection by the loop Mode d injection Equilibration Loop injection 04 48 D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com cm User Manual 33 4 70 03 73 01 After having launched the method RUN the equilibration phase starts That allows to pack the column with the solvents used At the end of the time or at a click on START the 6 ways electric valve switches on run position the sample is pushed in the chromatography column by the solvents pumped and the method starts Make your injection and then press Yes FLOW CHART PF450 RUN Injection port 38cm spring 32cm ka kN 100cm 60cm 39cm Ba I 56cm spring 50cm Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55
3. E i r3 dd I ee A Sak a ALS GL KE S 1 sue kee eR EG AN amp d m nou rm E C g mg Tr FN w gz a U Once the user interface opened several graphic elements allow to see in real time the state of the instruments and the evolution of the working parameters The application has been developped with the aim of being convivial and intuitive Refer to the Instruction Manual supplied for more explanations on the software use Use the stylus on the tactile screen to create methods in the software and control the system Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr 4 2 Makea liquid injection FLOW CHART PF450 The liquid injection is done by the automatic injection valve module with the loop the volume is predefined from 100uL LOAD amp EQUILIBRATION to 40mL or more The loading and injection can be done at any time during a Injection port method when the pump runs or not Here is the procedure ML 32cm v The 6 way electric valve is on the LOAD position during a method creation and column equilibration v Fill a syringe with the volume of sample chosen and place itin the Adapter Stainless steel for injection port n 1 Keep the syringe on this adapter to avoid the leak of the sample uu T i i 3 PI
4. 2 ferrules v Binding column v General power cable model according to the destination country v USB key including the file of installation exe of the freeware Interchim Software Demo v Stylus amp Keyboard v Columns starting kit 2x PF 15SIHP 25G 2x PF 30SIHP 25G 2x PF 50SIHP 25G v Documentation User Manual for PURIFLASH 4750 system Instruction Manual for Interchim Software Adjusting Protocol of Assembled Unit for Flash 06 DAD UV detector PDF document Operational Qualification and test report My documents start for Interchim Software Installation alific ation x 211 bis avenue 4 gtion Qu BE 4140 05100 K ntlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com P0300 LASH 33 4 70 03 73 01 3 Installation 3 1 Assembling the system Select a suitable location by ensuring adequate air space 15cm around the system to provide ventilation of the controller the pump the detector cooling fans Its total weight is approximately 66kg Air extractions v Assemble the system in the following order Fraction collector 25kg 6 Ways Electric Valve 5kg Unit control 15kg Detector 7kg Pump 8kg Pre column Holder 2kg Column Holder 3kg Solvent tray 1kg Put then the stainless steel solvent tray supplied on top to dispose solvents up to five They cover the totality of the system with a drainage system and th
5. PF4360 24 acquisition channel P N PF4320 FLASH ELSD P N F10320 Funnel rack set 1 unit P N FJ4331 or Funnel rack set 4 units P N FJ4330 10 Safety solvent caps kit 4 units P N DV2760 11 Safety waste cap with container 5L filter P N 106930 or Safety waste cap with container 20L filter P N JO4500 12 Drainage system of the tray collector P N DZ7790 13 2 columns selection switching valve P N LO6600 14 Backflush valve P N LO6610 15 2 columns selection switching valve Backflush valve P N LO6620 16 2 fraction collector coupled P N JV0950 17 RI IOTA 2 P N FSQ600 18 Mass Spectrometer interface P N JO1090 19 CarouXel P N JO3750 20 Autosampler P N LO8850 21 Ballasting kit P N DZ7360 22 Stainless steel loops 23 Racks set O NOOO A C N gt Designation Items 1 Solvent level sensors 5 1 global 3 Enhancement tube device for 13x100mm tubes Communication interface 5 1 cable Sensors cables x 5 Straps 80cm x 5 Removable cable ties x 5 Power adapter Hardware Installation procedure 4 Video camera TLC plates holder Webcam with USB connection Bracket CD for Webcam drivers installation TLC plates holder Hardware Installation procedure 1 Solvent level sensors 5 5 separated pr 5 Fume enclosure Communication interface 5 5 cab
6. PFS800 connected to Splitter box ETFE Tubing 1 8 ID 1 6mm Column Outlet Splitter Inlet length 72cm Adapter 74 28F 10 32M P N 737664 connected to Splitter box Manometer P N FJ6720 option 2 red or white tubing to connect the gas 6mm OD 2 meter 1 meter and fitting 1 PEEK fitting 10 32 Male fitting Inlet ELSD P N 780771 Adapter 1 4 28Female 1 4 28 Female Nylon P N 187210 only for the puriFlash SPOT II Union PEEK 10 32M 1 4 28F P N 737664 Internal reducer 1 16 1 8 P N 229980 only for semi prep and prep configuration on puriFlash 4100 puriFlash 4250 250 puriFlash 4250 40 Installation procedure 9 Funnel rack set 1 unit or Funnel rack set 4 units 10 Safety solvent caps kit 4 units 11 Safety waste cap with container 5L filter or Safety waste cap with container 20L filter 12 Drainage System of the tray collector 13 2 columns selection switching valve Switching valve ETFE tubing Installation procedure 14 Backflush valve D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Bainterchim Backflush valve ETFE tubing Installation procedure 15 2 columns selection switching valve Backflush valve 2 valves ETFE tubing Installation procedure 16 2 fraction collector coupled Fraction collector General power cable Serial communication cable Sub 09 male female Tubing with 2 fitings
7. 6 3 Replacing a seal 6 4 Cleaning replacing a check valve 6 5 Replacing the detector deuterium lamp 6 6 Cleaning the detector flow cell 6 7 Cleaning the 6 ways electric valve replacing the rotor 7 gt Preventive measures Upkeep schedule page A 38 8 gt Warranty page A 39 8 1 Generalities 8 2 Limited warranty of consumable parts 9 gt List of soare parts Blow up views page A 42 10 gt Specifications page A 50 Appendix Rack sets available page A 50 Appendix 2 Service report page A 51 Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr 1 gt General description The PURIFL SH 450 system is a modern instrument designed for Flash chromatography which allows to withdraw mobile phases from four containers of solvents form a gradient inject liquid or solid samples and provide the solution to a chromatography column detector measures the absorbance and gives a signal called chromatogram which is visualized with the built in computer and the control software INTERCHIM Software installed Then fractions can be collected with the fraction collector The characteristics of a teg ierg 4750 are v Flow rate Up to 250mL min v Pressure Max 50 bar 725 psi v Gradient Quaternary Air purge v Column size From F4 to F1600 format accordi
8. and 2 ferrules I 3m 1 8 ID 1 6mm 17 RI IOTA 2 1V Output cable Power cable ETFE Tubing 1 16 ID 1mm RI outlet fitting 10 32 Male P N 780770 UV inlet fitting and ferrule for 1 16 tubing P N 167155 and AB8790 length 70cm Adapter 1 4 28 Female 1 4V 28 Female Nylon P N 187210 only for the puriFlash SPOT II User s Manuel Installation procedure 18 Mass spectrometer interface 19 CarouXel Power cable RS 232 cable Installation procedure 20 Autosampler Power cable RS 232 cable User s manuel Installation procedure 21 Ballasting kit for 1 8 tubing 5 units 22 Stainless steel loops 9 different loops 100uL 250uL 500LL 1mL 2mL Dm 10mL 20mL 40mL 23 Racks set 13x100mm P N PF4370 16x150mm P N PF4380 18x150mm P N PF4390 21x150mm P N PF4410 25x150mm P N PF4420 28x180mm P N PF4430 29 5x200mm P N PF4440 Plateform rack for bottles P N LV5200 T l 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH LA 33 4 70 03 73 01 4 gt Operation 4 1 Control the system When all the installations and connections are made switch on the PURIFL SH 4 50 After few seconds and the initialisations of different instruments the control software Interchim Software automatically starts Note the good starting no message Communication Error is necessary for the correct working of the system
9. disconnect it from the electrical supply It will be reconnected only when the panel will be installed back in its place 3 Let the lamp cools down fifteen minutes after switching it off 4 Remove tubing and connections from the detector module and remove it from the whole system you must first remove the pump module 5 Position the detector module on its side in order to view the bottom and unscrew the three M3 cylindrical head screws indicated below using an Allen wrench 2 5mm Take care not to lose M4 flat washers 6 Put the detector in the upright position and take off the front plastic cover 7 Then unscrew the two M4 domed head screws located on the back of the module using an Allen wrench 3mm and take off the top sheet cover 8 Loosen the screw on the socket of light tube behind the cell compartment using T 20 Torx adapter Turn the lamp socket to the horizontal position and tighten the same screw to fix it again 9 Take the lamp power supply cables and disconnect the 3 pin white connector Loosen and remove the two screws which hold the lamp using T 10 Torx adapter D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CTI ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH Lf 33 4 70 03 73 0 Never touch quartz bulb of the lamp with bare fingers to avoid damages causing faulty working of the detector 10 Remove the lamp from the lamp socket and provide yoursel
10. due to mechanical force to the unit v The guarantee will be cancelled in case of failure or damage due to acts of nature v The guarantee will be cancelled in case of failure or damage due to non compliance with safety procedures v The customer is liable for transport risks In case of damage during transport the beneficiary must issue all reserves to the transport firm before accepting the delivery of the device v The guarantee will not cover damage caused in accidents external events contingencies or force majeure due to negligence or a lack of surveillance by the customer or due to non compliance with safety rules v The guarantee will not cover damage if the maintenance procedures recommended by the manufacturer are not complied with v Interchim will not guarantee the results of the use of the PURFLASH 77 50 D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PURIFLASH 8 2 Limited warranty of consumable parts Item Fittings unions amp tubing TEFZEL ferrule with Stainless steel lock ring for 1 8 tubing PEEK 1 4 28 short nut for 1 8 tubing for GV1690 PEEK 1 4 28 long nut for 1 8 tubing for GV1690 Stainless steel ferrule for 1 8 tubing Stainless steel nut for 1 8 tubing PTFE Plug 1 4 28 for gradient valve ETFE Plug 1 4 28 Adapter PEEK Luer Lock Male to 1 4 28 Female option Quick
11. from the electrical supply 3 Remove the plastic cover to gain access to the pump heads 4 Disconnect the Suction and Delivery tubing and fittings 5 Remove the two screws Allen wrench 3mm Carefully unscrew alternating from one to the other 6 Carefully remove the pump head The piston and the seal are now visible 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE GKAL T l 33 470 03 88 55 Fax 33 4 70 03 82 60 www interchim com User Manual interfine interchim fr 6 3 Replacing a seal There are in all four seals one of every head and one of every cleaning disc You can Piston seal exchange them by removing the pump heads and cleaning disc as explained previously 1 Remove the seal using a small screwdriver Be sure to avoid scoring any part of the pump head and the cleaning disc as a scratch will prevent proper sealing If the seat is scored it is necessary to replace the head or the cleaning disc and these are not under warranty 2 Thoroughly clean the seal seat with alcohol to remove possible seal particles and plunge the new seal in propanol 2 for lubrication 3 Place the new seal into the pump head or the cleaning disc and provide yourself with a special tool sold with the first set of seals P N PFS040 4 Position the part A on the head and slip the part B into A Then hit gently with the fist on the part B Tool 2 Parts 5 Remove the tool an
12. load assembly tightening it into the valve body by hand just beyond the point where it touches the rotor and put the screw which maintains the valve body use a allen wrench English 9 64 4 Cycle the valve 3 times to seat the sealing surfaces leaving the valve fully 5 Tighten the pre load assembly in quarter turn increments cycling the valve 5 times with solvent to seat the sealing surfaces Remake this step until you have no leak CAUTION Make certain that the valve is never left partially actuated It should always be in either its fully clockwise or fully counterclockwise position D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE JuGteddluh re 33 470 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH 4L 0 33 4 70 03 73 01 7 Preventive measures Upkeep schedule By considering the use of clean solvents and all precautions already mentioned in the chapter 6 Maintenance here is a table which regroup the recommended actions in order to assuring a good working of the system Operation Frequency Pump Weekl Check the suitable tightening of fittings strongly hand tight and the proper or ied inaccuracy flow rates state of tubing not bended solvents leaks or air bubbles are observed Yearly Replace the tubing and associated fittings or when damaged parts are observed As often as possible Clean and rinse the gradient valve with suitable solution specially afte
13. ret thus seras seeds uet Rn rennes vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenedebaredy Bru ne hop Signature Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e gl ie M See Module ACTOR PIN and description of defective spare ln e Any further action ee EI EE RI NPs Signature Service report lt O Installation End of warranty visit O Warranty O Repair O Maintenance O Out of warranty System description ee EEE ED AESA E EEES EEE nE Error rr nrr ereenn reenen v ME Module nemes EEG gt CO ENE EE Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty Systemi description esis r o SEE Ville AC NE NE SE eee ere PIN and description of defective SD e Any further action required Leia ce senos Una rax ret thus seras seeds uet Rn rennes vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenedebaredy Bru ne hop Signature Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e ele e ns NN ie ee ST M Module ACTOR MC T PIN and descr
14. rotor 6 Ways electric valve 2 positions Alimentation V1 6 Ways electric valve 2 positions Driver V1 6 Ways electric valve 2 positions Rotor Pump Collector fuses 1 6A 220V x2 Pump Collector fuses 2 5A 110V x2 General Central Processing Unit Touch screen USB hub USB extension cord I 0 5cm PFS520 PFS530 PFS540 FK2400 PFS550 LV9380 PFS570 PFS590 PFTO10 PFT100 PFS600 PFS610 PFS620 PFS630 PFS640 PFS650 PFS660 Stylus AC power supply 230 24V 150W UC axial fan V2 Multi stage performance EMI filter General ON OFF switch General fuses x 2 3 15 A 220V General fuses x 2 6 3 A 110V Serial communication cable male female Serial communication cable female female Distribution power cable male female for main voltage Distribution power cable male female for 24V Power cord FR Power cord UK Power cord US D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCE ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com Lr PUPPI LASH CH 33 4 70 03 73 01 10 gt Specifications v Model name PURIFL ASH 4750 system Serial number XXXX X XX v Flow rate range 1 to 250ml min v Flow rate performance 1 accuracy with H O degassed at 20 C Repeatability better than 0 596 v Gradient former Linear response from 2 to 98 v Injection Automatic liquid or solid injectio
15. screw 104960 Stainless Steel bulkhead union 90 PES370 9 Adapter ETFE Luer Lock Female to 1 4 28 Male or PFS780 2 Adapter Stainless Steel Luer Lock Female to 1 4 28 Male GV1680 2 PEEK Ring Outlet column holder 102690 3 Binding column 102700 6 D D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE LG KAA re 33 470 03 88 55 Fax 33 4 70 03 82 60 www interchim com POGUE LASH SC 33 4 70 03 73 01 Item Part Number Pre column holder TEFZEL ferrule with Stainless steel lock ring for 1 8 tubing GV1690 PEEK 1 4 28 long nut for 1 8 tubing for GV1690 GV1710 Adapter Luer Lock Male to 1 4 28 Male PEEK PFS770 4 Guidance part for large black POM holder FO4400 3 5 Stainless steel union for flash configuration FV6560 7 Large black POM holder grub screw 104960 6 9 Semi prep and Prep adaptation kit RAA190 Stainless Steel bulkhead union 90 PFS370 5 Adapter ETFE Luer Lock Female to 1 4 28 Male or PFS780 2 Adapter Stainless Steel Luer Lock Female to 28 Male GV1680 2 PEEK Ring Outlet column holder 102690 3 Screw pre column holder 109140 6 Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell re 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual Item Part Number N 6 Ways electric valve GO9040 104970 107070 109130 PFS980 PFS990 6 Ways electric valve 2 positions valve body
16. stop luer check valve option Kel F mounting stud option Adapter Luer Lock Male to 1 4 28 Male PEEK Adapter ETFE Luer Lock Female to 1 4 28 Male Adapter Stainless Steel Luer Lock Female to 1 4 28 Male Adapter Stainless Steel for injection Back pressure regulator 20psi 1 4 bar PEEK tee union for 1 8 tubing Stainless steel bulkhead union 90 PEEK union 10 32 female Suction tube ETFE 1 50m 1 8 ID 2 4mm PEEK nut TEFZEL ferrule spring 20cm x 4 V2 Tube Gradient valve to Tee pump inlet ETFE 28cm 1 8 ID 2 4mm 2 PEEK nuts 2 TEFZEL Ferrules spring 20cm V2 Tube valve port n 3 to Column holder inlet ETFE 60cm 1 8 ID 1 6mm 1 PEEK nut 1 ETFE Ferrule 1 SS nut 1 SS Ferrule Tube valve port n 5 to Pre column holder inlet ETFE 38cm 1 8 ID 1 6mm 1 PEEK nut 1 ETFE Ferrule 1 SS nut 1 SS Ferrule spring 32cm Tube Pre column holder outlet to valve port n 2 ETFE 56cm 1 8 ID 1 6mm 1 PEEK nut 1 ETFE Ferrule 1 SS nut 1 SS Ferrule spring 50cm Tube valve port n 6 to waste ETFE 100cm 1 8 ID 1 6mm 1 SS nut 1 SS Ferrule Tube Column holder outlet to detector inlet ETFE 73cm 1 8 ID 1 6mm 2 PEEK nuts 2 ETFE Ferrules Tube Detector outlet to collector inlet ETFE 37cm 1 8 ID 1 6mm 2 PEEK nuts 2 TEFZEL Ferrules Waste outlet tube ETFE 1 50m 1 8 ID 1 6mm PEEK nut ETFE ferru
17. with three stainless steel racks for 18 mm diameter tubes as standard That represents in all 176 tubes and a volume of 5 6 Litres D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Dui ella T l 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com The computer and its touch screen The system is controlled by the central processing unit via a touch screen which replaces the keyboard and the mouse The operator uses the control software and can create methods to run the instrument Touch screen USB Ports Power light This module contains the main ON OFF switch and centralizes the connection for data and power supply to the other modules Four USB connectors are also available 1 in front of the system under the screen and 3 to the left hand side cable maximum length 2m The external connections Some others external connectors allow to plug in additional devices like second detector or options R f HV1200 Multi Com 2 Ports Box The maximum length of all external cables connected to the system is 2 metres LAN plug Local Area Network Serial communication port Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com 33 470037301 _ User Manual interfine interchim fr Optional Level sensors plugs External controls Analogic signal inputs 2 Packing list Stand
18. 140 03100 Montlu on FRANCE Duell T l 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PGE L FT C Kai NS 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Dui ella re 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com Item Pump suite Piston 8 5 x 65 kit if cleaning disc Cleaning discs x 2 Head support bearing Head support reinforcement Camshaft 2 Cams Compression spring Pump opto sensor cable Opto sensor disc Camshaft holder parts Balancer kit Balancer cable 33 4 70 03 73 01 Part Number PF S080 102970 104720 104740 104750 PFS100 PFS180 PFS190 104760 FO4170 FO4190 N 11 12 8 10 Belt Pulley big camshaft Pulley small motor 104770 104780 104790 interfine interchim fr Item Pump suite Motor Black Motor holder Motor driver Motor black Axial fan kit AC Power supply 230 24V 60W Support sheet Absorber Pump Collector fuses 2 5 A 110V x 2 Pump Collector fuses 1 6 A 220V x 2 Mixing chamber Seal for mixing chamber Frit 20um for mixing chamber x 2 Pressure transducer seal Pressure transducer cable Leakage sensor Detector Flow cell 0 3mm Deuterium lamp Axial Fan Power Board Detector fuse 500mA 250V D O 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Bainterchim User Manual Part Number N 101940
19. 2 PEEK nuts 2 TEFZEL Ferrules Tube Detector outlet to collector inlet ETFE 37cm 1 8 ID 1 6mm 2 PEEK nuts 2 TEFZEL Ferrules Waste outlet tube ETFE 1 50m 1 8 ID 1 6mm PEEK nut ETFE ferrule Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com GV1690 GV1700 GV1710 GV1720 GV1730 PFS730 PFS740 PFS750 PFS760 PFS770 PFS780 GV1680 107060 FO3980 PFS710 106960 106980 106990 107000 107010 107020 107030 107050 107040 PFS870 interfine interchim fr User Manual Item Part Number N Tube Collection valve to collection needle ETFE 1m 1 8 ID 1 6mm 1 PEEK nut 1 TEFZEL Ferrule spring pipe Spring for tubes 5cm PFS880 PFS800 PFTO30 Spring for suction tubes 20cm Valves Quaternary Gradient valve Air purge CPU2010 HO2530 PFS910 GO9040 Collection 3 way valve 6 ways electric valve 2 positions valve body rotor Electronic boards Electronic board CPU2010 Electronic board Collector 102950 PFS500 System Flex for collector connections PFS490 Pump Check valve 8mm PFS920 4 Check valve housing 8mm PFS020 6 Check valve 10mm PFS930 3 Check valve housing 10mm PFS010 2 Seal 8 5mm PFS940 5 Head PFS030 1 Tool for insertion of seals D 8 5mm PFS040 D 211 bis avenue JF Kennedy BP 1
20. 6cm x 1 6mm ID tube insert one end into the pump outlet and the other end into the valve n 4 of the 6 ways electric valve Position the tubing as shown below and tighten each fitting Pump outlet Valve port n 4 D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell re 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH LLS 33 4 70 03 73 01 Valve port n 3 to column holder Using the 60cm x 1 6mm ID tube insert one end into the valve n 3 of the 6 ways electric valve and the other end into the column holder intlet Position the tubing as shown below and tighten each fitting Valve port n 3 Column holder inlet Valve port n 5 to pre column holder Using the 38cm x 1 6mm ID tube insert one end into the valve n 5 of the 6 ways electric valve and the other end into the pre column holder inlet Position the tubing as shown below and tighten each fitting Valve port n 5 Pre column holder inlet Pre column holder to valve port n 2 Using the 56cm x 1 6mm ID tube insert one end into the Pre column holder outlet and the other end into the valve n 2 of the 6 ways electric valve Position the tubing as shown below and tighten each fitting Valve port n 2 Pre column holder outlet Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine int
21. Ai ni En E T T H j L LO EN i d E HELGE Lo 4 S INTERFINE CHEMICALS ANALYTICAL SCIENCES BIOCHROMATOGRAPHY BIOSCIENCE INNOVATION Di d b i j 1 et UF E F i F ST WM 1 7 es ah I pa PURIFLASH 4150 E User MERI ELT ITM 2012 10 31 Ultra Performance Flash Purification Crossover flash prep LC Lab Scale v Flash columns up to F800 format v Prep LC columns up to 80mm id 15um E aa a PU LASH 250 ml min up to 50 bar INTERFINE CHEMICALS ea j nte re h m BP 1140 03100 Montlu on Hotline 33 4 70 03 73 01 interfine interchim fr Fax 33 4 70 03 82 60 211 bis avenue JF Kenned PURIFLASH 33 4 70 03 73 0 Summary 1 gt General description page A 3 2 gt Packing list page A 7 3 gt Installation page A 8 3 1 Assembling the system 3 2 Install the tubing and prepare the containers 3 3 Set up the column the dry load the pre column amp the loop 3 4 Prepare the fractions collection 3 5 Position the stylus 3 6 Make the connections electrical and data transmission 3 Semi prep amp prep adaptation kit 3 8 Options and accessories 4 gt Operation page A 24 4 1 Control the system 4 2 Make a liquid injection 4 3 Make a solid injection 5 gt Safety regulations page A 29 6 gt Maintenance page A 30 6 1 Introduction 6 2 Removing a pump head
22. Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty Systemi description esis r o SEE Ville AC NE NE SE eee ere PIN and description of defective SD e Any further action required Leia ce senos Una rax ret thus seras seeds uet Rn rennes vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenedebaredy Bru ne hop Signature Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e ele e ns NN ie ee ST M Module ACTOR MC T PIN and description of defective spare ln e Any TERE ap eto N S 10 111 EE RI NPs Signature Service report lt O Installation End of warranty visit O Warranty O Repair O Maintenance O Out of warranty System description ee EEE ED AESA E EEES EEE nE Error rr nrr ereenn reenen v ME Module nemes EEG gt CO ENE EE Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty Systemi description esis r o SEE Ville AC NE NE SE eee ere PIN and description of defective SD e Any further action required Leia ce senos Una rax
23. Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr 4 3 Make a solid injection FLOW CHART PF450 The solid injection is done by the automatic injection valve LOAD amp EQUILIBRATION module Injection port The solid injection is done via a dry load in which the solid sample has been previously deposited The dry load is emplaced directly on the pre column holder 38cm spring 32cm The loading and injection can be done at any time during a method when the pump runs or not Here is the procedure v The 6 way electric valve is on the LOAD position during a method creation and equilibration of column Place the dry load on the pre column holder as shown below 1 Unlock the black ring of the fitting Adapter PEEK Luer lock male to 74 28 male of the pre column holder 2 Move up the large black POM holder on the vertical guidance rail and place the bottom fitting of the dry load or pre column on the Adapter ETFE or Stainless steel Luer lock female to 74 28 male located on the stainless steel bulkhead ee Tes union 90 56cm pom 3 Move down the large black POM holder and lock again spring 50cm i the black ring of the fitting Adapter PEEK Luer lock male to 74 28 male to the top fitting of the dry load In the panel Settings of the method edited select v Equilibration in the section Injection Mode According to the type of the column a time an
24. ard system urR FLASH 4750 Item v PURIFL ASH 2750 system composed of Pump module Detector module 6 Ways electric valve module Fraction collector module Pre column holder Computer amp power supply module Column holder module Y Tubing with fittings ETFE OD 1 8 Suction 1 50m ID 2 4mm x4 with 20cm spring Gradient valve outlet to Tee Pump inlet 27cm ID 2 4mm present on the system Pump outlet to Valve port n 4 46cm ID 1 6mm Spring 39cm Valve port n 3 to Column Holder inlet 60cm ID 1 6mm Valve port n 5 to Pre column Holder inlet 38cm ID 1 6mm Spring 32cm Pre column Holder outlet to Valve port n 2 b6cm ID 1 6mm Spring 50cm Valve port n 6 Waste outlet 100cm ID 1 6mm Valve port n 1 Stainless Steel Adapter for injection Column holder outlet to Detector inlet 73cm ID 1 6mm Detector outlet to Fraction collector inlet 37cm ID 1 6mm Waste outlet 1 50m ID 1 6mm Pressure test OQ PEEK 70cm 1 16 ID 0 5mm v Rack set PF4390 for 18mm diameter tubes 4 racks v Semi prep and prep adaptation kit P N GO2400 v PEEK union 10 32 female P N NR0860 v Solvent Tray with a drainage system Serial communication cables 3 x Sub D9 male female 1 x Sub D9 female female Distribution power cables 3 x main voltage supply 1 x 24V v Fittings for cleaning discs connections 2 long nuts
25. ct sample automatically liquid or solid via dry load via 6 ways electric valve directly to the chromatography column installed Guidance rail Pre column x 9 Ne Column or dry load Knurled Knob The adjustable height holder of the large black POM holder allows to adapt all types of columns from 4g to 16009 and pre column from 4g to 3009 knurled knob can block this holder on the vertical guidance rail The bottom union is fixed Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual The UV diode array detector This instrument measures the amount of light absorbed by a liquid passing through the cell at a given wavelength It is equipped with a deuterium lamp The two wavelengths available and the SCAN function are selected within a large range from 200 to 600nm or from190 to 840nm in option P N GO9630 FL s x lg 3 e E n Deuterium lamp 2 er e BE A EX li Flow cell The fraction collector The fraction collector is placed downstream of the detector After the detection of the signal chromatogram the results obtained at the outlet of the column peaks can be directed to tubes disposed on racks A built in 3 way valve allows to choose between the collection or the rejection of the liquid Needle Electronic board collector The collector is provided
26. d a flow rate are selected by default They depend on the column parameters but you can modifiy them if needed v Loop injection for the injection by the solid deposit Mode d injection Equilibration Loop Injection After having launched the method RUN the equilibration phase starts That allows to pack the column with the solvents used At the end of the time or at a click on START the 6 ways electric valve switches on run position the sample in the dry load is pushed in the chromatography column by the solvents pumped and the method starts Make your injection and then press Yes D D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Daci elltil re 33 470 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH L de 33 4 70 03 73 01 FLOW CHART PF450 RUN Injection port N 38cm spring 32cm 100cm 60cm spring 39cm 56cm spring 50cm Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual 5 gt Safety requlations This equipment is built according to the E U security standards However risks and dangers could remain if the system is used in a different way from which it is intended or if it is used by untrained personnel The PURIFLASH 4750 should only be used by laboratory trained or experienced pe
27. d note that the seal is well placed in the pump head or in the cleaning disc 6 Finally replace the cleaning disc and the head on the piston and put its two fixing screws with care alternating from one to the other 7 Connect the Suction and Delivery tubing and fittings Note The life time of the seals is dependent on the flow rate pressure type of liquids used and temperature but mostly on the cleanliness of the mobile phase and sample The presence of micro particles will cause accelerated wear and tear of the seals Similarly any dried buffer particles on the piston will damage the seal D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH Lf 33 4 70 03 73 01 6 4 Cleaning replacing a check valve Abnormal pressure fluctuation or the observation of pump noise on the data system is usually due to check valve problems The noise is regular in nature and is due to pressure and flow variation Pump accuracy is also compromised Details on check valve repairs are given below There are four check valves two per head one for each inlet model 10mm P N PFS930 and one four each outlet model 8mm P N PFS920 If they become dirty they will not open and close correctly for a long time 1 Purge the pump head with a suitable solvent or alcohol 1 propanol or 2 propanol 2 Switch off the module and disconnect it from the el
28. e connector in the suitable socket label DET of the unit control Screw the other end round female connector in the back panel of the detector Link the unit control label COM2 DETECTOR and the detector module using the Sub D9 female female cable supplied Do not forget to set the connectors by fastening the two fixing screws Back panel of the pump Serial port gt COM1 Pump Switch 0 1 8 pin connector Axial fan ENN j Fuses drawer Power plug Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr Take the power cable for main voltage supply and connect it at the same time in the plugs of the unit control label PUMP and in the sockets of the pump module Link the unit control label COM1 PUMP and the pump module using the Sub D9 male female cable supplied Do not forget to set the connectors by fastening the two fixing screws Back panel of the fraction collector External controls Switch 0 1 Serial port gt COM3 Analogic i t Fuses drawer inputs for second detector acquisition Power plug Take the power cable for main voltage supply and connect it at the same time in the plugs of the unit control label COLL and in the sockets of the pump module Link the unit control label COM3 COLLECTOR and the collector module using
29. e hydraulic circuit rinsed and the inlets outlets corked The instrument must be carefully carried in its original box 6 Maintenance 6 1 Introduction This chapter describes actions that should be performed on a routine basis to ensure long term safe and trouble free operation of the system The frequency of the maintenance activities is dependent on the nature of the application the solvents used the quantity of the mobile phase delivered by the pump the level of cleanliness of the facility etc The pump has been designed for reliability and needs very little routine maintenance when operated correctly The following figure represents the liquid connections of the pump 2UCtON tubing Check valve housing Delivery tubing Tee urson Firing D Bulkhead union Reduced accuracy of the pump may be due to seal wear or to a fault in the check valves This section provides information and procedures about how to replace consumable parts and how to maintain the instrument The removal of protective panels may only be performed by an authorized person 6 2 Removing a pump head When disassembling or reassembling the pump make sure that each component is clean and take care that the system is assembled in a clean environment Remove it if you need to replace its seal details hereafter 1 Purge the pump head with a suitable solvent or alcohol 1 propanol or 2 propanol 2 Switch off the module and disconnect it
30. ectrical supply 3 Remove the plastic cover to gain access to the pump heads 4 Disconnect the Suction and Delivery tubing and fittings 5 Unscrew the check valve housing using a spanner 17mm 6 Clean the check valves or provide yourself with news The cleaning allows to remove the particles present in the valves by placing them in an ultrasonic bath with methanol or acetone during approximately five minutes Ensure that the arrow is directed downwards during the cleaning Else damage to the surface quality or the ball and the seat will occur 7 Replace the check valves Whether it is for the inlet 10 mm or the outlet 8mm make sure the arrow is always directed upwards Inlet Suction Outlet Delivery Check valve housing Check valve 8 mm Check valve 10 mm CEDEGE 8 Hard tighten the valve housing in the head until hearing a little crunch which means that the new check valve is enough tight and the imperviousness properly done This noise corresponds to the collapsing of the top and bottom membranes 9 Connect the Suction and Delivery tubing and fittings Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr 6 5 Replacing the detector deuterium lamp 1 Purge the system with a suitable solvent with then alcohol and with air 2 Switch off the system and
31. eir design avoids any leakage risk Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCCAIN re 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr Fix the column holder and pre column holder as below Column holder Pre column holder Insert the column holder at the bottom Insert the pre column holder of the collector at the bottom of the collector Put the binding column and the screw on the middle of the column holder Put the screw at the top of the pre column holder 3 2 Install the tubing and prepare the containers The tubes situated outside the housing are delivered apart in order to avoid all deterioration during transfer they are already equipped with the suitable fittings nut ferrule to connect them easily to the system Regarding the main circuit two sizes of tube are used 1 8 ID 2 4mm for suction 1 8 ID 1 6mm for others D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com RE PUPPI LASH A SC 33 4 70 03 73 01 Follow the instructions below to correctly install the tubing Suction Provide yourself with the four Suction tubes I21 5m 1 8 ID 2 4mm supplied they are more brittle than others so take care not to bend them For the tube A take the extremity where there is a fitting with a long sprin
32. elon 1 propanol or 2 propanol during approximatively Usea pencil type magnet available from Valco hree minutes or any electronic components supplier unscrew 2 Purge the system with air during one minute completely the entire knurled pre load assembly 3 Switch off the system and remove the rotor with a pencil type magnet 4 Unscrew the screw which maintains the valve body Switch on the system In this case don t pay use a allen wrench English 9 64 unscrew partially attention to parts 4 and 5 the pre load assembly with a plier CAUTION Do not tamper with the preset socket adjustment screw SOCKET ADJUSTMENT TO SCREW PRELOAD ASSEMBLY E o Remove the valve body and finish to unscrew e the pre load assembly 6 Remove the rotor from the valve body as shown below CAUTION Any contact between the interior of the valve body and the metal of the rotor or any tool used is likely to cause damage Once the rotor is removed note the orientation of the rotor tab which is marked with an ID letter denoting the type of seal material ROTOR TAB ID LETTER interfine interchim fr User Manual Cleaning the valve body 1 N Wet a cotton swab with a solvent which is compatible with the chromatographic system Isopropyl alcohol is recommended Gently swab the polished interior of the valve to remove any loose residue Blow with clean compressed gas to remove any
33. erchim fr Valve port n 1 Screw the Stainless Steel adapter for injection into the port n 1 of the 6 ways electric valve and tighten Adapter stainless steel for injection Valve port n 6 Using the 1m x 1 6mm tube attach the end with the fitting into the valve n 6 of the 6 ways electric valve and tighten On the other end insert into an appropriate waste bottle Waste tube valve port n 6 Column holder to detector Using the 73cm x 1 6mm ID tube insert one end into the column holder outlet of the stainless steel bulkhead union 90 and the other end into the detector inlet Position the tubing as shown below and tighten each fitting Detector inlet Column holder outlet D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com P038 LASH L 33 4 70 03 73 01 Detector to fraction collector Using the 37cm x 1 6mm ID tube insert one end into the detector outlet and the other end into the fraction collector inlet Seat the ferrules and hand tighten each fitting Detector outlet Fraction collector inlet Waste Using the 1 5m x 1 6mm tube attach the end with the fitting into the fraction collector waste port and hand tighten On the other end insert into an appropriate waste bottle 3 3 Set up the column the pre column the dry load and the loop A large range of columns can be used on the column ho
34. f with a new one 11 Carefully insert the new lamp assuring that it is correctly positioned Should you touch the glass of the lamp accidentally clean it thoroughly with a lint free cloth and alcohol 12 Screw it in securely and connect the plug in the 3 pin white socket 13 Reassemble the unit by making the same steps but in the reverse order After the lamp replacement recalibration and zeroing of the counter of operating hours must be carried out cf Service Info 39 6 6 Cleaning the detector flow cell A contaminated cell in lowered light transmission may increase noise level and make Flowcel difficulties at zeroing The simplest cleaning method is flushing dismantled cell with suitable solvents The cell must be taken out from the instrument before washing 1 Follow the instructions 1 to 7 described in the previous chapter 6 5 Replacing detector deuterium lamp in order to properly disassemble the system 2 Disconnect the outlet tubing from the cell PEEK nuts 3 Remove easily the cell from the system by tilting the locking lever placing one hand under the cell to prevent it from falling to the bottom and tilting the locking lever with the other hand 4 Select the solvent type according to character of contamination by a series of mutually miscible solvents It is possible to use both organic and inorganic solvents and diluted solutions of acids 5 Flush the cell with pure solvent and rinse extens
35. for external diameter Install it as follows pd Move up the large black POM holder on the vertical guidance rail and place the bottom fitting of the dry load or pre column on the Adapter ETFE or Stainless steel Luer lock female to 74 28 male located on the stainless steel bulkhead union 90 1 Unscrew lightly the knurled knob of the holder in order to allow its vertical movement 4 Move down the large black POM holder and lock again the blazzack ring of the fitting Adapter PEEK Luer lock male to 74 28 male to the top fitting of the dry load 2 Unlock the black ring of the fitting Adapter PEEK Luer lock male to 4 28 male of the pre column holder Set up the loop Injection port 38cm spring 32cm El 46cm spring 39cm I 56cm spring 50cm Connect the loop into the 6 ways electric valve between the port n 2 and n 5 as shown below and thighten with wrench 74 5 16 D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH Lf 33 47003 7301 3 4 Prepare the fractions collection According to the type of collection required small or big volumes short or long fractionation etc the equipments to install are different The standard rack set 18x150mm supplied is PF4390 composed of four racks for 176 tubes in all it allows a total collection volume of 5 6 Litre
36. g and put the ferrule in the PEEK body of the suction valve Screw the PEEK nut tighten it hand tight and position properly the long spring Renew the same operation for B C and D as indicated on the photo The Air inlet stays free Outlet dee S Gradient V d C valve body PEEK nut To ensure the pumping continuity of the solvents and avoid the air entry in the hydraulic circuit the others extremities can be equipped with ballasts in option P N DZ7360 It consists in fact to put a fitting nut set ferrule and screw it in a stainless steel piece which serve of weight to maintain the tubes at the bottom of the containers PEEK nut Ballast Position the solvents containers on the solvent tray for instance and put the ballasted tubes into them Gradient valve The gradient valve to Tee Pump inlet tube is already connected into the pump I 27cm 1 8 ID 2 4mm as shown below Tee pump inlet Pump outlet Gradient valve outlet Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCCAIN re 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr 6 Ways electric valve fluidic connection The fluidic connection on the 6 ways electric valve module is explained below Injection port 38cm spring 32cm 100cm 45om spring 39cm z 56cm spring 50cm Pump to valve port n 4 Using the 4
37. iption of defective spare ln e Any TERE ap eto N S 10 111 EE RI NPs Signature Service report lt O Installation End of warranty visit O Warranty O Repair O Maintenance O Out of warranty System description ee EEE ED AESA E EEES EEE nE Error rr nrr ereenn reenen v ME Module nemes EEG gt CO ENE EE Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty Systemi description esis r o SEE Ville AC NE NE SE eee ere PIN and description of defective SD e Any further action required Leia ce senos Una rax ret thus seras seeds uet Rn rennes vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenedebaredy Bru ne hop Signature Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e gl ie M See Module ACTOR PIN and description of defective spare ln e Any further action ee EI EE RI NPs Signature Service report lt O Installation End of warranty visit O Warranty O Repair O Maintenance O Out of warranty System description ee EEE ED AESA E EEES EEE nE Error rr nrr ereenn reenen v ME Module nemes EEG gt CO ENE EE Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O O
38. itable tightening Blocked passage due to uncleanliness of installation or unsuitable mobile phase Check valve 8mm PFS920 6 months Check valve 10mm PFS930 6 months Piston 8 5 x 65 kit PFS080 1 year Seal 8 5mm PFS940 6 months gt Terms of use Use of clean solvents and clean containers Follow up of the preventive maintenance Respect precisely the maintenance procedures gt Damages not covered Damaged piece due to unsuitable use assembly or replacement Blocked passage due to uncleanliness of installation or unsuitable mobile phase Detector Flow cell 0 3mm PFS960 1 year 6 months Deuterium lamp PFS970 or 1000 hours gt Terms of use Use of clean solvents and clean containers Respect precisely the maintenance procedures gt Damages not covered Damaged piece due to wrong use or replacement or inappropriate solvent pumping Blocked passage due to uncleanliness of installation or unsuitable mobile phase D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PURIFLASH 33 4 70 03 73 01 9 gt List of spare parts Blow up views Item Part Number N Fittings unions amp tubing TEFZEL ferrule with Stainless steel lock ring for 1 8 tubing PEEK 1 4 28 short nut for 1 8 tubing for GV1690 PEEK 1 4 28 long nut for 1 8 tubing for GV1690 Stainless steel ferrule for 1 8 tubing Stainles
39. ively 6 Reassemble the module by carrying out the Locking same steps but in the reverse order Flow cell M aff lever After the operation check cell cleanness purging C T the system and verify there is no baseline drift User Manual interfine interchim fr 6 7 Cleaning the 6 ways electric valve replacing the rotor The cleaning allows to remove the particles present in the valve by following the instructions below LOAD amp EQUILIBRATION Injection port Cleaning the 6 ways electric valve 38cm 1 Switch on the system 2 Purge the system with a suitable solvent or alcohol 1 propanol or 2 propanol during approximatively three minutes The 6 way electric valve is on the LOAD position during a purge gt gt gt gt gt gt gt gt gt spring 50cm Injection port 38cm spring 32cm 3 Click on the loop in the software to switch the position of the 6 ways electric valve and clean the RUN position containing the loop or the solid deposit 1 46cm spring 39cm gt gt gt gt gt gt gt gt gt e 56cm spring S0em D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CGT ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH LL gt E 33 4 70 03 73 0 4 Stop the purge Disassembling the valve 1 Purge the system with a suitable solvent or alcohol ATIGHUSUSSSIBUpOSSIDINDEHIO TEMOVE WE F
40. lder columns packed with normal or reverse phase with an external diameter up to 120mm The Extension kit for Large columns P N DU9671 is supplied with this device this overall dimension can go up to 120mm On the pre column holder dry load and pre column 4g up to 300g can be used A large range of stainless Steel loop injection from 100uL to 100mL or more can be used on the 6 ways electric valve Set up the column 2 m Move up the large black POM holder on the vertical guidance rail and place the bottom fitting EN ofthe column on the Adapter ETFE or Stainless Eu steel Luer lock female to 4 28 male located on the stainless steel bulkhead union 90 1 Unscrew lightly the knurled knob in order to allow its vertical movement 4 Move down the large black POM holder and lock again the black ring of the fitting Adapter PEEK Luer lock male to 14 28 male to the top fitting of the column M ae Unlock the black ring of the fitting Adapter PEEK Luer lock male to 74 28 male located on the column holder Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CCE ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr Set up the dry load and pre column To inject solid sample make dry loading injection or use the pre column you have the possibility to use a column of which the maximum dimensions are 27cm in height and 70mm
41. le Side panel Sensors cables x 5 eo oh door Straps 80cm x 5 5 screws Removable cable ties x 5 Collar Power adapter Magnetic flap Hardware Hardware Installation procedure l E Installation procedure 2 Bar code reader Reader with USB connection Bracket Hardware Installation procedure 6 Quad system Module for 4 additional columns installation Inlet tubing with stainless steel fitting set 1 8 ID 1 6 mm 1 50m Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual Outlet tubing 1 8 ID 1 6 mm 1 50m PEEK 4 28 long nut for 1 8 tubing x3 ETFE ferrule for 1 8 tubing x 3 CD for TRP converter drivers installation External control cable USB cable Power cable model according to the destination country Hardware Installation procedure 7 2 Acquisition channel Module USB 1408FS USB cable USB key for drivers installation 8 FLASH ELSD FLASH ELSD 1V Output cable Power cable Splitter box with micro needle option ETFE Tubing 1 16 ID 0 25mm Splitter Outlet ELSD Inlet length 30cm fitting 10 32 Male P N 780771 connected to Splitter box ETFE Tubing 1 8 ID 1 6mm oplitter Outlet UV Inlet length 72cm Adapter 74 28F 10 32M P N 737664 spring 20cm P N
42. le GV1690 GV1700 GV1710 GV1720 GV1730 PFS720 PFS730 PFS740 PFS750 PFS760 PFS770 PFS780 GV1680 107060 FO3980 PFS710 PFS370 NR0860 106960 106980 107000 107010 107020 107030 107050 107040 PFS870 33 4 70 03 73 01 Part Number Warranty period Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCCAIN re 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfineQinterchim fr User Manual Item Part Number Warranty period Fittings unions amp tubing suite Tube Collection valve to collection needle ETFE 1m 1 8 ID 1 6mm 1 PEEK nut 1 TEFZEL Ferrule PFS880 3 months spring pipe gt Terms of use Normal tightening of the fittings Use of clean solvents and clean containers Good positioning according to the instructions given in this manual Damages not covered Damaged thread due to unsuitable tightening Blocked passage due to uncleanliness of installation or unsuitable mobile phase Bended or cut tube due to unsuitable positioning Valves Quaternary Gradient valve Air purge CPU 2010 HO2530 Collection 3 way valve PFS910 6 ways electric valve 2 positions valve body rotor GO9040 gt Terms of use Normal tightening of the fittings Use of clean solvents and clean containers Follow up of the preventive maintenance actions gt Damages not covered Damaged thread or crushed internal part due to unsu
43. licking on the loop in the software with Isopropanol then with the less polar solvent 1 Unscrew peek 74 28 Long Nut 1 Luer male with lock to 1 4 28 male 2 and Stainless Steel Luer female to 1 4 28 male 3 Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell re 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr 2 Replace by semi prep adapter inlet 1 Screw the inlet adapter on half of the thread amp oulet 2 column and screw peek 7 28 Long Nut 3 3 Put the semi prep column push the Stainless Steel tubing in the column and screw the PEEK fitting 1 Note There is a specific adapter for the prep column Operation is the same than for the prep column connection remove the column and install the PEEK union 10 32 female P N NR0860 AN CAUTION To purge the system in semi prep configuration D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CTI ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH of 33 4 70 03 73 0 3 8 Options and accessories Solvents level sensors 5 1 Global P N FSP720 or Solvents level sensors 5 5 Separated P N FSP730 Bar code reader P N GO2380 Enhancement tube device for 13 x 100mm tubes P N DW3360 Video camera TLC plates holder P N GO2390 Fume enclosure P N PF4350 Quad system P N
44. lint left by the swab Visually inspect the interior of the valve body The conical surface should appear highly polished If any scratches are visible between the ports or anywhere which might suggest a potential leakage path or wear source the valve should be returned to the factory for regrinding and polishing Cleaning the rotor 1 AUN Carefully grasp the rotor on either end and briefly immerse it in solvent If it is difficult to grip the rotor securely hemostats or needle nosed pliers may be helpful Grip the tab end being careful not to mar the metal or touch the polymer Gently wipe the polymer with a clean tissue Blow with clean compressed gas to remove any lint left by the tissue Visually inspect the rotor If it shows any scratches and or a narrowing of the flow passages replacement is necessary Assembly new or used rotor 1 Place the valve body and orient it so that it Another easier possibility properly engages in the mechanism to remove the rotor The valve body is correctly placed when If you have a pencil type magnet the adapter injection port is turned toward don t pay attention to parts 1 the top and go directly to parts 2 2 Insert the clean or new rotor into the valve body again being careful that the tab doesn t touch the polished interior of the valve body Make sure the rotor tab is fully inserted into the slot in the driver see figure below 3 Replace the knurled pre
45. n v System protection Software Pressures Min Max adjustable Leakage detection v Maximum pressure 50 bar 725 psi v Control via PC software INTERCHIM SOFTWARE VX X v Storage up to 32 methods in software memory plus 160Go of hard disk memory v AC mains supply voltage 220 240 VAC 50 60Hz or 110 120VAC 50 60Hz v Electrical protection Delayed action fuse 3 15A or 6 3A v Power distribution TT or TN power system only transient over voltages according to installation category II Class 1 equipment requires mains socket plug with protective earth terminal v IP Code IP20 v Dimensions 540 x 770 x 480mm Width x Height x Depth without connection v Weight 66kg v Temperature 5 to 40 C v Others Indoor use Altitude up to 2000m Pollution degree 2 Noise level 35dB gt Appendix 1 Rack sets available As mentioned in the chapter 3 4 Prepare the fractions collection several racks are usable with the fraction collector Here is a recapitulative list indicating their designation the features of tubes used and the total capacity of the rack set SHORT RACKS a rack set includes 4 racks Designation Tubes size Tubes volume Tubes by rack Tubes by rack set Total volume 18 x 150mm 18 x 180mm PF4390 PF4370 13 x 100mm PF4380 16 x 150mm PF4410 21 x 150mm PF4420 25 x 150mm PF4430 28 x 150mm PF4440 29 5 x 200mm Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE
46. nd then tighten the set screw When the system is turned on the arm will return to its home position Collection tubing Dispense arm Dispense head Needle Tightening screw 3 5 Position the stylus The stylus is supplied to control the system via computer touch screen A fixed holder allows to easily put away it when it is not used and its extensible cord linked to the holder gives the suitable length Stick the stylus holder just as you like in order to profit at best of its features i e to the left side of the system as showed on this picture D D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE JuGteddluh re 33 470 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH Lf 33 4 70 03 73 01 3 6 Make the connections electrical and data transmission The complete system is supplied with appropriate fuses and is ready to operate at the line voltage of the shipping destination The Computer module serves as the primary point for power distribution and data collection The ports on the modules are labelled to ensure proper connections Using the cables provided connect each module to the unit control Unit control Back panel of the unit control communication ports Power sockets Detector Back panel of the detector 24 V round connector E34 Axial fans Serial port gt COM2 Take first the special cable for 24 V supply and screw the round mal
47. ng to column trademark v Automatic injection valve module Liquid or solid injection v UV Detection Spectrophotometer dual wavelength DAD technology range 200 600nm v Collection Several types of racks available The complete system consists of following modules The 2 head pump As mentioned above the pump can deliver mobile phase at flow rates up to two hundred and fifty millilitres per minute with low residual pulsation It is a high performance double piston pump reciprocal movement Thanks to a system pulleys belt a stepper motor drives two special cams on which lean the two pistons Their motion generates a cycle and then creates the suction A static mixing chamber at the outlet ensures a suitable mixing of solvents whatever the working flow rate it is associated with a pressure transducer Mixing chamber Cleaning disc Leak sensor Electronic board CPU 2010 Pressure transducer D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CGT ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PURIFLASH 33 4 70 03 73 01 Moreover an optic sensor can detect leakages in the pump drain pan The pumping is then interrupted and the user informed via the control software The 4 ways suction valve allows to perform quaternary elution gradients A fifth way can be used for Air Purge The injection unit and columns holder system This device gives the possibility to inje
48. r having pumped aqueous solvents or strong buffers Replace seals and backup rings Yearly Clean the check valves Quarterly Yearly or when abnormal pressure flow rate fluctuation or noisy baseline are observed Replace the check valves Quarterly Clean the piston rods or when pump head is disassembled Detector E Quarterly Check the efficiency of the deuterium lamp or when higher noise levels or decreased sensitivity are observed When its efficiency is Replace the deuterium lamp unreliable Clean the flow cell Quarterly General Verify the cleanliness of all liquid containers Daily Weekly Check the suitable tightening of fittings strongly hand tight and the proper or when inaccuracy flow rates state of tubing not bended solvents leaks or air bubbles are observed Clean the injection valves and all parts in contact with solvents or samples As often as possible with suitable solutions Rinse abundantly using a mixing of water and specially after having pumped alcohol aqueous solvents or strong buffers Check the tangential fan working to maintain a good air recycling inside the different modules Monthly Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com Manual interfine interchim fr 8 gt Warranty 8 1 Generalities Interchim guarantees the PURIFL SH 450 for one
49. rsons The user should inform the distributor in case of problems and safety related matters that occur during the use of the instrument Alerts you to potential hazardous situations that could WARN N G result in serious injury or damage of the system This system is designed for chromatographic purposes It must be operated using appropriate solvents and within specified ranges for pressure flows and temperatures as described in this manual If it is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired When handling potentially hazardous solvents and samples it is important to follow standard laboratory safety procedures Common chemicals used in the laboratory can be toxic corrosive flammable or a combination of these properties A list of all safety aspects is outside the scope of this manual but the following points should be considered v Ensure adequate ventilation v Wear eye protection v Wear gloves when handling chemicals v Ensure all liquid containers are secure v No smocking or naked flames v Clean up all spillages W ARN N G Eye damage could occur from particles or chemicals Use proper eye protection EYE HAZARD If a line ruptures or a valve opens accidentally under pressure a potentially hazardous spray of liquid might be generated by the pump Never open a solvent line or valve under pressure Stop the pump first and let the pres
50. s modified or changed by the customer or the service provider without Interchim s approval parts damaged by this modification parts not recommended by Interchim parts subject to wear and consumables v The guarantee will not cover any parts not listed in part 8 2 v The guarantee will not cover electrical and or electronic and or IT incidents caused by external factors v The guarantee will not cover damage caused to the software or hardware due to contamination by an IT virus v The guarantee will not cover damage or failures caused by impact and or bad weather v The guarantee will be cancelled in case of damage caused by abnormal mechanical forces applied to the device and exceeding the limits defined in the user guide v The guarantee will be cancelled in case of corrosion to the device due to solvent leakage or samples v The guarantee will be cancelled in case of corrosion to electronic components caused by highly corrosive gas v The guarantee will not cover damage or failures caused by assembly dismantling modification or transport after initial installation v The guarantee will be cancelled in case of failure or damage due to non compliance with the closing and shutdown procedure for the device v The guarantee will be cancelled in case of failure or damage due to wrong installation v The guarantee will be cancelled in case of failure or damage due to wrong AC power supply v The guarantee will be cancelled in case of failure or damage
51. s steel nut for 1 8 tubing ETFE Plug 1 4 28 Adapter PEEK Luer Lock Male to 1 4 28 Female option Quick stop luer check valve option Kel F mounting stud option Adapter Luer Lock Male to 1 4 28 Male PEEK Adapter ETFE Luer Lock Female to 1 4 28 Male or Adapter Stainless Steel Luer Lock Female to 1 4 28 Male Adapter Stainless Steel for injection Back pressure regulator 20psi 1 4 bar option PEEK tee union for 1 8 tubing Suction tube ETFE 1 50m 1 8 ID 2 4mm PEEK nut TEFZEL ferrule spring 20cm x 4 V2 Tube Gradient valve to Tee pump inlet ETFE 28cm 1 8 ID 2 4mm 2 PEEK nuts 2 TEFZEL Ferrules spring 20cm V2 Tube Pump outlet to valve port n 4 ETFE 46cm 1 8 ID 1 6mm 1 PEEK nut 1 TEFZEL Ferrule 1 SS nut 1 SS Ferrule spring 39cm Tube valve port n 3 to Column holder inlet ETFE 60cm 1 8 ID 1 6mm 1 PEEK nut 1 TEFZEL Ferrule 1 SS nut 1 SS Ferrule Tube valve port n 5 to Pre column holder inlet ETFE 38cm 1 8 ID 1 6mm 1 PEEK nut 1 TEFZEL Ferrule 1 SS nut 1 SS Ferrule spring 32cm Tube Pre column holder outlet to valve port n 2 ETFE 56cm 1 8 ID 1 6mm 1 PEEK nut 1 TEFZEL Ferrule 1 SS nut 1 SS Ferrule spring 50cm Tube valve port n 6 to waste ETFE 100cm 1 8 ID 1 6mm 1 SS nut 1 SS Ferrule Tube Column holder outlet to detector inlet ETFE 73cm 1 8 ID 1 6mm
52. s with 180 mm high tubes AN CAUTION Before any action take a diagonal plier and carefully cut the cable tie which holds the arm during the transfer Dispense arm Cable Tie Place first the tubes in the holes and put then the racks on the tray Thanks to notches machined on racks and positioning guides already mounted on the system their emplacement is easy and necessarily proper Positioning notch d a Well placed rack Back guides Front guides You can also equip your collector with an optional Enhancement tube device for 13x100m P N DW3360 It is designed for elevating the racks with small height tubes Its installation is simple too because you have just to emplace it on the collector tray using positioning guides Several models of rack set are available find a detailed list on APPENDIX 1 Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr It is important to maintain appropriate spacing of the dispensing needle above the tubes A space of 5 25mm between the end of the needle to the top of the tube is recommended To adjust the needle height turn off the system remove the racks and gently move the arm into an easy to access position Do not use the needle to relocate the arm as bending and mis alignment may occur Loosen the set screw and adjust the height of the needle a
53. sure drops to zero Wear protection glasses Hazardous voltages are present inside the WARN I N G instrument Disconnect from main power before removing screw attached panels SHOCK HAZARD D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Dui ella ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com P080 LASH LL 33 4 70 03 73 01 Removal of protective panels may only be performed by an authorized person When it is necessary to use a non original power cord plug make sure the replacement cord adheres to the color coding and polarity described in the manual and local building safety codes Replace blown fuses with fuses of the size and rating stipulated in the manual Replace faulty or frayed power cords immediately with the same type and rating Make sure that voltage sources and line voltage match the value for which the instrument is wired To ensure your own safety thus of your co workers and safe operation of the equipment observe the following instructions v Use a 3 wire mains socket with ground connection grounding is necessary to ensure operator safety and proper operation v Take care of inflammable solvents Store the system after having passed a clean solvent in the hydraulic circuit when not in use Do not leave buffer in the system it may cause blockages and damage the seals on start up v Before moving the instrument the external connections have to be disconnected th
54. the Sub D9 male female cable supplied Do not forget to set the connectors by fastening the two fixing screws 6 ways electric valve module Back panel of the 6 ways electric valve Vo Cr M LIES i CC Zeie mm Take the power cable for main voltage supply and connect it at the same time in the plugs of the unit control and in the sockets of the pump module Link the unit control COM4 and the pump module using the Sub D9 male female cable supplied Do not forget to set the connectors by fastening the two fixing screws Plug the AC power cable supplied in the general switch located to the left side of the system and after to the mains knowing that the connector depends also on the destination country comply with country regulations in effect D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCE ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com LN PUR LASH L 7 33 4 70 03 73 01 For American system 110 120 VAC 50 60 Hz 6 2 A delay action fuses 2 x 6 3 A For European system 220 240 VAC 50 60 Hz 3 1 A delay action fuses 2 x 3 15 A ON OFF Switch Fuses Drawer Power Cable Check that all the ON OFF switches of secondary modules pump detector collector are on I and switch on the unit with the main ON OFF switch 3 7 Semi prep and prep adaptation kit AN IMPORTANT Before installing the semi prep kit you must clean the system and the loop by c
55. ut of warranty Systemi description esis r o SEE Ville AC NE NE SE eee ere PIN and description of defective SD e Any further action required Leia ce senos Una rax ret thus seras seeds uet Rn rennes vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenedebaredy Bru ne hop Signature Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e ele e ns NN ie ee ST M Module ACTOR MC T PIN and description of defective spare ln e Any TERE ap eto N S 10 111 EE RI NPs Signature Service report lt O Installation End of warranty visit O Warranty O Repair O Maintenance O Out of warranty System description ee EEE ED AESA E EEES EEE nE Error rr nrr ereenn reenen v ME Module nemes EEG gt CO ENE EE painterchim 211 bis avenue J F Kennedy T l 33 4 70 03 88 55 BP 1140 03100 Montlu on Fax 33 4 70 03 82 60 Hotline 33 4 70 03 7301 E mail interfine interchim fr Not contractual pictures
56. year for parts and labour at the discretion of Interchim in normal conditions of use and installation from the date of signing of the installation report by the service provider approved by Interchim to install the machine and by the customer The PURIFL SH zz 50 device is guaranteed against material and manufacturing defaults in normal conditions of use by approved professionals and within the technical characteristics compatible with the functions defined in the user manual Terms and conditions of the guarantee v The device is only guaranteed if installed by a service provider approved by Interchim v The guarantee covers the supply of parts found defective by Interchim free of charge as a minimum within the limits of the parts listed in part 8 2 v The device must have been used in normal operating conditions and in accordance with the instructions v The device must have been used with the consumables recommended by Interchim v Exhaustive list of original parts covered by the guarantee part 8 2 Il Guarantee exclusions v The guarantee will not cover devices installed by the customer or by a service provider not approved by Interchim The guarantee will not cover equipment used in a manner which is non compliant with the provisions in the instructions The guarantee will not cover equipment subject to interventions repairs or modifications by personnel without Interchim approval v The guarantee will not cover part
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