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1. File Edit Reagents Setup Variables Password Procedure Name test2 ines Step Source Output Vol ml min Sense Cannula Depth mm 0 1 Cannula No a above bottom of tube 2 No 3 No Mix Cycles 2 4 No 5 No Mix Speed 30 6 No 7 No Mix Volume ml 5 8 No 9 No ug No Condition column 11 No Load sample onto column 12 No Rinse column Collect fraction 13 No Purge and Clean cannula 14 No Pause Dry Column 15 No Add reagent to Mixing Vessel 16 No Mix Reagents in Mixing Vessel 17 No 18 No 19 No 20 No OK Save Help 19 The upper right of the Create Procedures screen will also change as above Enter the appropriate values for your procedure 20 Change the value in the Mix Cycles box to 0 This will cause no mixing of the sample to occur Cannula Depth mm above bottom of tube Mix Cycles Mix Speed Die Mix Volume ml P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 30 RapidTrace Software Add Reagent to Sample and Mix Continued 21 Click in the second box of the first line directly under the word Source The source is what will be added to the sample The list that appears in the lower right contains source choices The items in the list were created when the reagent names were specified in the Reagent Setup screen See Defi
2. cccsccssscsssccsscsssesssenssesssessssssscssssssssesssesssescsssssssssscscssscsssessnessnesens 1 Unpack the Modules and Check the Parts cesscsscsseseceseeecsseceeesecaeesecnaeseceaeeceaecaeecessecaeeaecneveessaeeeesnaeereeaeeneaeeas 1 Dit PREPAaratlOM ANE E E E Fosedevtueoed sea vuoedsct svada bearnoobscovveus seuveevbe cduetan EE A 1 Installing Rapid Trace Hardware cscccsscssssssscssscesssssscssscssssssssnsssnessesssesssesessnessesssesssesssssssssesscessoess 4 Fav gntV G0 4 1 810 a senner eer cree pice trees tra E RA petra ER E R peepee A R E error rear Ea 4 Installing Rapid Trace Softwarre ccscccccsssscsscssscccsccccesscesscccecccccesceesssceessccscscccsesceesssscessccesesceesscsseeees 7 Installing the SOfWaATE seisein reer ron coer teeter errs eter reer tee ies Eea a ea rea rrerren terme terre ror eae 7 RapidTrace Software Overview ssscssscssssssssssssssssscssscssscsssssscscsscssssnssssessnesenessessssnessneseesssscsscossooess 9 Software Character Sti Sunsin nnee Sede tence EE stansens Ea A tatiana ident eee eee 9 ACCessinpithe SOMWALES esis scecszecizschestasecasstavesdsscsteaus ia r EEr eR E ES E E E E EEEE SE EE E IAT EE S 10 L ading the Fart ware sorreran ieira seeren anA a Eea e EEES seo eh EEEE hd Ea EEAS E VEETEE 10 RapidTrace Parts and Functions eesssoesooessoessosssosssosseosssosssosssosssosssosssossssssssesssesssesssesssesssesssesssesssoses 12 Front Feature S ocen erR E EEE ATE
3. The syringe valve and drive direct the flow of reagents under pressure and the turret and rack may move unexpectedly during operation Always keep body parts hair jewelry and clothing away from the turret and the rack Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Parts and Functions 13 Table 1 Front Parts and Functions Item Part 1 Gas Valve 2 Mixing Vessel 3 Syringe Drive and Liquid Co ON A U 10 11 12 13 14 Sensor 12 Port Valve Thumb Wheel Switch Power Light Run Light Error Light Column Plunger Start Stop Switch Cannula SPE Column Turret Service panel Standard Rack Function Activates gas drying of the column bed if the valve is plumbed into the unit and the Dry Column step is used in software Creates a mixed reagent when the Add Reagents to Mixing Vessel and Mix Reagents in Mixing Vessel steps are used in the software Performs all dispense and aspirate functions The syringe draws up either sample or reagent through the 12 Port Valve as specified in the procedure and dispenses it back through the 12 Port Valve to the column plunger or cannula as directed by the procedure If you set Liquid Sense in your procedure to Yes this sensor detects the presence of liquid in the syringe If it does not detect liquid an
4. Guaranteed 888 88 SOURCE www artisantg com ee RapidTrace Software 31 ee The liquid sensor should not be turned on for volumes of 1 2 mL or less If the sensor does not detect liquid it assumes that the reagent has run out The sensor sends a signal to stop the procedure and light the error light 26 Switch the sensing option off by clicking again on Yes The sensor is off when the field shows No Your procedure should look like this w Create procedure using reagent names for all modules File Edit Reagents Setup Variables Password Procedure Name TEST2 SPE Step Source Outpt Vol mimin 5 1 cogs ah e NOTE i Flow ranges are 0 36 mL min to 42 mL min and 006 mL sec to 0 7mL sec For more information on flow rates refer to the Flow Rate Conversion Chart p 89 P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 32 RapidTrace Software Condition Column 1 Click once in the Step box for the second row This line represents the second step in the procedure The lower right corner of the screen will display options for procedural steps 2 Click on Condition Column This step will appear in the cell 3 Click once in the source box for the second row The source is the reagent used to condition the SPE column Notice the secondary screen
5. 20 RapidTrace Software Create Save and Activate a Reagent Setup Topics covered in this section are Reagent Setup Reagent Setup screen Defining variables for the Reagents Saving the Reagent Setup file with a set extension Recalling a saved set file and making it the Active file Assigning Reagent setups to individual modules Screen The main functions of the Reagent Setup screen are Naming reagents and wastes and their abbreviations Defining the sip speeds of reagents The sip speed is the speed at which the syringe will pull the reagent out of the bottle It is recommended that the default sip speeds be maintained The speed range is 006 mL sec or 36 mL min to 0 7 mL sec or 42 mL min The default setting is 0 5 mL sec or 30 mL min Setting the column tubing volume The column tubing volume is the amount of liquid present in the line between the 12 Port Valve and the column plunger Use 0 4 mL when the tubing for gas drying is not connected Change this value to 0 9 mL when the gas valve is plumbed into the fluid path Refer to on line Help for further information Reagent setup for all modules File ModuleNumber Jol x Reagent ReagentName Abbreviation Number aN ona ono Waste Number default entries Sip ml min Reag1 30 Reag2 30 Reag3 30 Reag4 30 Reag5 Reag6 Reag Reag8 Waste Nam
6. Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Gas Lines Adding or Moving RapidTrace Modules 71 Attach the Manifold Piece Continued 77 Connect the line for reagent 1 from the new module to the manifold Figure 15 Use the labels in the startup kit to help identify lines Attach reagent line i Neighbor module New Module Figure 15 Connecting Two Modules Together 78 Continue placing manifolds on the workstation and connecting them to each successive reagent line until all eight reagents have been completed 79 After appropriately cleaning the check valves place them in the new reagent bottles If gas drying of the column bed is being used follow the instructions below to connect the gas line to the module Connect a Gas Manifold Connect a gas manifold to the gas line on the rear of the module that is being added as per the instructions on the previous page Attach the Manifold Piece steps 1 through 4 The gas line should already be attached to the gas tank NOTE The 1 8 in 3 175 mm tubing is for the gas line the 1 16 in 1 59 mm tubing is for reagents Connect an Individual Gas Line The startup kit has a 10 foot gas line two types of fittings to connect the gas line to a gas supply and an individual gas fitting 1 Attach the 1
7. A rtisan Artisan Technology Group is your source for quality TecmoogyGroup new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment 7 EQUIPMENT DEMOS HUNDREDS OF InstraV ea REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED D a gaa tia Contact us 888 88 SOURCE sales artisantg com www artisantg com C E RapidTrace SPE Workstation Installation and Quick Reference Manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Zymark Factory Warranty Zymark warrants your Zymark manufactured equipment including hardware software and firmware against defects in material and workmanship for a period of one 1 year from the date of s
8. After saving any changes close the screen and return to the Main menu P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 36 RapidTrace Software Carryover Prevention Carryover is a type of contamination that occurs when some of a sample remains in the system which can carryover into the next sample The fluid path transports both reagents and samples therefore you should check for carryover as part of transferring a method to the RapidTrace workstation The strongest organic reagent should be used when cleaning and purging the cannula This will most effectively clean the fluid path of any remaining sample NOTE L If the sample contains proteins i e plasma or serum samples a Purge and Clean Cannula step should be performed with water prior to performing a Purge and Clean Cannula step with a strong organic solvent This water rinse will help remove protein from the fluid path Proceed with caution when using protein samples to prevent any protein precipitation The following is a general procedure to determine carryover 1 Runa blank sample one that contains no analyte 2 Runa sample that contains a very high concentration of analyte 3 Run two additional blank samples 4 Analyze the blank samples for carryover of analyte The carryover measured is from the entire proc
9. For your active modules the test2 spe procedure should be assigned to Samples 1 through 5 and the 1mL spe procedure assigned to Samples 6 through 10 The above screen shows a two module workstation with the modules addressed as numbers 1 and 2 NOTE n If modules are switched off and or are not attached to the Controller no procedures will appear assigned to those modules To RUN an individual module click on the Run button for that module ce _ Double clicking on the Run button is considered a STOP command To STOP an individual module click on the Stop button for that module To run all modules click on the Run All button in the lower left corner To stop all modules click on the Stop All button If different procedures are assigned to individual modules the Run All button will not be available for use To return to the Main menu click on the Exit button in the lower right portion of the screen Follow these steps to run your procedures 1 From the Main menu click on Setup Racks The box in the lower left corner will show that all modules are selected K Module 0 P Module 5 IX Module 1 X Module 6 X Module 2 X Module 7 X Module 3 X Module 8 IX Module 4 x Module 9 P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 44 Running Methods and Monitoring Workstation Opera
10. liquid above plunger syringe base Turn the glass barrel to reset the Teflon seal Restart run If syringe continues to leak replace the syringe Syringe leaking at base Remove knurled nuts from bottom of syringe to remove black plate and syringe base Reseat the syringe base and reassemble Restart run If syringe continues to leak replace syringe amp Attempt to determine the cause of the syringe leakage and resolve the problem before restarting the run The most common reasons are a Particulate in the sample b Flow rate in the procedure is too fast causing excessive pressure If the particulate in the sample caused the problem evaluate the initial sample preparation to prevent this from happening If the flow rate is too fast reduce the flow rate P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 80 Service Troubleshooting Continued Problem Solution Clogged fluid path at 1 Runa test sample using water to determine exactly where the clog cannula column plunger occurs or waste station i 2 Download and run the appropriate Error Recovery plunger routine from the Zymark Internet Home Page www zymark com Before running fill a sample test tube and the mixing vessel with 2N NaOH Also place the waste lines in a beaker of 2N NaOH
11. Phase Polymeric Resin Reverse Phase Rinse Silica Soft Gel Sorbent Syringe Barrel Wash Refers to the chemistry occurring when polar interactions such as hydrogen bonding dipole dipole and a variety of other interactions occur between the functional group of the sorbent group and the bonds of the analyte Common normal phase sorbents are CN NH2 20H and Si The material used to achieve the separation The size of the silica other packing material in the column In most SPE columns the particle size is 40 120 microns HPLC Columns are 4 microns and larger The type of packing material in the SPE column Some SPE columns are based on a solid phase made of polymers rather than silica Refers to the chemistry occurring when non polar interactions occur between the carbon hydrogen bonds on the functional group of the sorbent group and the carbon hydrogen bonds of the analyte Common reverse phase sorbents are C18 C8 CH and PH The step s used to flush the column with reagent after the sample is applied to remove unwanted species Sometimes called wash The base material used for the binding of functional groups Some SPE columns are packed with a gel based material rather than silica The material packed into the SPE column commonly a silica with a functional group bound to it Refers to the design of the SPE column used on the RapidTrace which looks like the barrel of a syringe
12. See following section 3 Assign a procedure that includes at least one Collect Fraction step to the code using the Magnetically Encoded Racks icon Encode the Rack To magnetically encode a rack the following items are needed e A RapidTrace rack e Kit of Magnets provided in your startup kit e A slotted flat blade screwdriver e The code number to be assigned Using this picture of the standard rack 13x100 sample tubes and 12x75 fraction tubes as a reference follow the steps below Screw hole Screw hole for magnet cover for magnet cover 12345 DO NOT USE THIS HOLE IF IT IS ON YOUR RACK 1 Remove the two screws from both ends of the rack and remove the magnet cover Below the cover are several holes for magnets The five holes used to encode the rack are shown above as shaded and are numbered 1 through 5 in the picture below The first hole which is directly next to the screw hole may not be on your particular rack However if the rack has this hole disregard it 2 Follow the Magnet table to properly code your rack Place a magnet in each of the shaded holes Replace cover and screws and secure into place P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 48 Running Methods and Monitoring Workstation Operations 3 Label your rack appropriately so you can identify eithe
13. Use the Procedure Description field to describe what the prime procedure does Procedure Description This procedure purges lines 1 4 with 6 mLs of reagent Water is the last purge reagent All waste generated from a Purge and Clean Cannula step exits from Waste Line 4 It is advisable to have this line in a waste reservoir that will contain mixed waste Both aqueous and organic waste will exit from this line as the lines are primed Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Daily Operations 53 Cleaning the RapidTrace Modules The following Post Run Cleanup procedure is recommended with routine maintenance Cleaning the modules removes any proteins that may have built up in the fluid path NOTE H Do not leave a workstation inactive for more than one day without cleaning Post run Cleanup Procedure NOTE H Both biological and organic wastes may contain proteins Itis very important that these waste lines be cleaned At least 50 mL water and at least 8 mL of both 2N sodium hydroxide NaOH and 2N nitric acid HNO3 will be required for use during the cleanup procedure Sodium hydroxide and nitric acid will eliminate protein buildup in the fluid path and neutralize each other Water will flush the pathway for effective cleanup Create procedure
14. Waste Lines Locate the plugs that came in the startup kit in the bag labeled Manifold pieces and plugs There should be nine plugs per bag Plug Locate the manifold pieces Figure 6 as specified in the dentify Reagent Plumbing Setup p 60 and detach them from the module Insert an extra plug in the opening There can be up to 9 manifold pieces per module e 8 manifolds for reagents 1 through 8 e 1 manifold for the gas line if gas drying is used Figure 6 Manifold Plumbing Piece Untwist the connector attaching the module reagent line to the manifold piece and attach a plug to that position on the manifold piece Continue placing plugs on all manifold pieces for the modules that are being relocated The four waste lines extend from a cutout at the base of the back of each module df Cutout for waste lines 1 Figure 7 Cutout for Reagent and Cannula Waste Lines The lines have a twist connection located several inches from the back of the module to make the module easier to move Locate the fittings and untwist the connection The 10 foot lines will remain in the waste receptacle You can now move the modules P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 64 Adding or Moving RapidTrace Modules Moving Modules with Individual Reage
15. software disks disk 1 P N 65071 disk 2 P N 65075 1 per module Startup Kit P N 59815 for one module Quantity ordered Any accessory kits ordered Site Preparation The Customer Site Preparation for the RapidTrace SPE Workstation document provides complete information regarding site preparation This document is shipped to the customer prior to delivery of the RapidTrace SPE Workstation When setting up the RapidTrace modules adequate space for reagents and storage space for waste receptacles located below the modules must be available Confirm that an appropriate location is available with gas if necessary and electrical sources as specified by these site preparation requirements before installing the RapidTrace SPE Workstation Instructions for installing additional modules can be found in Adding or Moving RapidTrace Modules p 61 DANGER OA A Never use hydrogen or other flammable gases Use of incorrect gasses may cause explosion or combustion P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 2 Unpacking and Site Preparation Computer Requirements Windows NT 4 0 service pack 5 or greater U S version operating system with 16MB of RAM Space Minimum bench space for each module Height 23 in 58 5 cm Width 4 in 10 2 cm Depth 23 in 58 5 cm Controlle
16. www artisantg com 96 Index R Rack magnet tableiccs s isiisistsiaescstiesGedts deweaiesaistetsaseas 48 magnetically encoded 47 Rack IDrennnsn ene 46 Racks tUb snsnnrenn n R R 90 RapidTrace software installing sre ia veeseonceseehes dd aheegsiuoveasvacdeenes es 7 RapidTrace controller 0 0 ceeeeecceseeceeeeceeeeeenereeenee 1 Reagent Lities ss c0sisiceepessede cosets cecetasesctscssnseencceensoees 6 Reagent setup eceecceeseeceseeeseeceeeeeenaeceeeeeesaeceteeees 20 assigning TEAGEN 0 eee eeeeeeeeceeeeeceeeeeeeeeeeeeeeeneees 24 defining variables 21 SAVINO erea N a ates 22 Repair depot ccccecccescesecseeeeeeeeeeeeeeeeeneseneeneenaees 85 S Sip speed e eseseeeeeesesereseseesersrererserereserersrrseerese 20 26 Site preparation ssseeeeeseeeieresestsrsrteresrrstssrrereesese 1 power TEQUIFEMEMNES 0 0 eeececeeeeeceeeeeeeeeeeseeeeneesees 3 Solid phase extraction SPE v Solvent compatibility chart 0cccescesseeseesteeseeees 88 Specifications MUSES 6 5 cba r eoivees swnavees A EEA 3 87 line frEQUENCY svo cssseceiccdeise gedeede teres ederiasecteane tee 3 line VOM ASC 5 cessiseclecsntsiecssesstseesiesastesesetoesesoeauncaoas 3 Support Services 0 0 cecececeesseeeeceeeeeeeceseceseesnecseeeseeenes 84 Symbols uSed cesccsseesseeceeseeeeeeeeceeceeeesseeeeesaeenaes vi T Table of symbols cecccecesseeseeeeeeeeeeseeeeeeeteeeteensees vi Technical Support Center
17. Air bubbles in reagent lines 1 Order Retaining clips from Zymark P N 38413 Tubing falls out of fittings Linesdonokstay primed 2 Install them on fittings to hold tubing in place O rings on column plunger 1 Inspect SPE columns Look for packing material on inside of column need to be replaced often Discontinue using those columns 2 For 1 mL columns order replacement O ring P N 60969 For 3 mL columns order replacement O ring P N 45096 Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Fuses Overview Service 81 The RapidTrace SPE Workstation is equipped with a universal AC power supply with a fuse block assembly that can be configured for either 110VAC or 220VAC If the workstation is already set for the line voltage of your country you do not have to alter the fuse block assembly This section describes how to replace fuses as well as alter the position of the fuse block assembly Replacing the Fuses Follow the instructions below to replace either the fuses with new similarly rated fuses 110 120 VAC model 200 220 VAC model Fuses 1 5A MDA quick acting 1 0 8A quick acting 2 P N 37645 P N 39838 DANGER OA Electrical shock hazard Switch the power OFF and disconnect the power supply before changing the fuses For continued fire protection and corr
18. Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 46 Running Methods and Monitoring Workstation Operations View Selected Report ce Only one report can be viewed at a time 1 Click on the cell of the report you want to view then click on the View Selected Report button Another screen will pop up that will display the Rack Layout and the Event Log View Report x Rack Layout Event Log Rack Sample Procedure tart Time 3 26 98 4 09 00 PM Position Tube test2 spe test2 spe StartS ample test2 spe Stopped test2 spe test2 spe Iml spe 1Imlspe Iml spe Iml spe 1Iml spe Exit Help 2 The rack layout in the box on the left identifies the procedures that are assigned to the samples and if a sample test tube is present If no sample test tube is present the procedure does not run on that sample In this example above all ten sample tubes were present 55 The event log shows the date and time the procedure ran a running log of the times each sample began when the module stopped and any errors that occurred 56 Inthis example the procedure was stopped 1 minute 6 seconds after it began The screen will also show a Rack ID representing the Code assigned to a magnetically encoded rack If a magnetically encoded rack was not used the Rack ID will show a 0 as shown above 57 Click on Exit to return to the Reports screen Pr
19. EEEE E E ERAS AEE EIEREN NE 12 Back Panel Features ccscsssssssssssssssssssessessssesessoesesssnscosssnsesnsssnsssnssnsessesuesssssssscsseossesssensssnssesessseeseeessersseassaess 14 External PIMOS riirn nen sess catasetvarsuseseetntsecoceedssuceceseieeesd A EAE EEA fuente 16 RapidTrace Softwar e cccccccsssssssssscssscssscsssssssenesssssssssessssesesessscssssssssssescscsssessnessnessnessnessesssesssesssoees 18 Main men Features siriene cei Bosco este levies a eens eee es ee ee ec 18 Create Saye and Activate a Reagent Setupec icccsccccscsacieiccsescssdsccsebacscvecsoiecsseeseacsadeucdens caedadnecsdesceeesddevssdecdvaneesedeens 20 Crcanne Procedure Seente Monee Sey a a nc sn dh tvescis vie tes east E ees uaa ieee eat 26 Writing an SPE Procedure sisemises nise sene irie ne E EER Ea eE EEEE EEES EN E Ee aini iins 28 Carryover Prevention sesei iorne kinira Enna EEE Ea EKRE reana EKEREN a ERTAS EAEra EEEE TENES EaP E ED EREEREER SE 36 Setup Procedure Sequence Soressi ierei e eriei sinea e EE EEEE EEE E EAEE EEEE E EAEE 38 Assigning SPE Procedures to Samples in the Workstation cccceccesescceseeseeseceeeeeceeeeceaeeeeeeceaeeneaeeaeeaeeaeeeaeeateaes 40 Running Methods and Monitoring Workstation Operations scsscssscssscesscessscssesssesssssssssseesees 42 ROM SCl6OD E EE E E E E E satavqde eatin easeeevyead E EOE ees 42 Running and Monitoring SPE Procedures on Up to Ten Modules eeceesesesesseeeeeceseceeeeceeceeeeseeen
20. Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 42 Running Methods and Monitoring Workstation Operations Running Methods and Monitoring Workstation Operations Run Screen Main Features The main functions of this screen are to load run and monitor the operation of modules gt Click on Run Monitor from the Main menu j Run RapidTrace _ oyx Beep Module 0 Module 1 Module 2 Module 3 Module 4 1 test2 spe 1 test2 spe 2 test2 spe 2 test2 spe 3 test2 spe 3 test2 spe 4 test2 spe 4 test2 spe 5 test2 spe 5 test2 spe 6 1ml spe 6 1ml spe 7 1mbLspe 7 Imlspe 8 1ml spe 8 1ml spe 9 1ml spe 9 1ml spe 10 1ml spe 10 1ml spe Run Stop Run Stop Run Stop Beep The Beep Control is accessible at the top left of the screen Beep allows a beeper to sound when a module encounters an error and when the modules in your workstation have completed the procedures Any number between 0 and 100 may be entered into the field This value represents the number of repeats per signal Test the beep to determine the preferred setting Entering a 0 will disable the option and no beep will sound cg Whena module has an error it will beep in the same manner as the test beep When all modules are finished running they will beep ten times longer than the test beep NOTE ou When using a RapidTrace
21. Monday through Friday from 8 00 a m to 8 00 p m EST For technical support in Europe contact Zymark at Runcorn UK 44 1928 711448 or fax 44 1928 791228 For more information contact your local Zymark representative Customer Support Plans Zymark offers a full range of support services From our original factory warranty through a comprehensive line of customer support plans Zymark offers Field Service Engineers and In house specialists who are dedicated to supporting customers with hardware software and application development needs Our programs can include such useful services as e preventive maintenance diagnostic servicing performed on site by Zymark field service engineers extended use of the Zymark Technical Support Center after hour weekend and holiday support repair depot servicing parts labor and travel expense coverage other customized services upon request Zymark Center for Training and Development Zymark Center for Training and Development offers training especially for RapidTrace users The course covers the basics of creating and maximizing Solid Phase Extraction SPE methods using the RapidTrace Workstation For more information contact Zymark by phone at 1 508 435 2634 USA or by fax at 1 508 435 3439 USA Our Internet address is training zymark com In Europe contact Zymark at Runcorn UK 44 1928 711448 or fax 44 1928 791228 For more information contact your local Zymark representative Zymark Co
22. No 12 No 13 No 14 No 15 No 16 No 7 No 18 No 19 No 20 No OK Save Help 2 At the top of the screen a blinking cursor will appear in the Procedure Name field Type test2 into that field Create procedure using reagent names for all modules File Edit Reagents Setup Variables Password Liquid Step Source Output Vol ml min Sense Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Software 29 Add Reagent to Sample and Mix 1 Click in the top left box in the first line of the Step column This will be the first step in the sample procedure Clicking on this field opens another screen in the lower right corner of the screen that shows your choices for steps in the procedure Add reagent to sample and mix Condition column Load sample onto column Rinse column Collect fraction Purge and Clean cannula Pause Dry Column Add reagent to Mixing Vessel Mix Reagents in Mixing Vessel 2 Click once on Add Reagent to Sample and Mix Add to Sample will appear in the first box Cannula will appear as a default under the Output column Create procedure using reagent names for all modules _ ol x
23. P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 66 Adding or Moving RapidTrace Modules Reconnecting Modules Connect the Communications Cable s 1 Connect one RS232 communications cable to the 9 pin connector on the Controller 2 Plug the other end of the cable into the bottom receptacle on the back of the first module 3 For additional units connect a second RS232 communications cable to the top 9 pin connector of the first unit 4 Plug the other end of this cable into the bottom receptacle of the next module Continue until all modules are connected together Connect the Power Cord Connect the power cords to the back of the modules and to the power source Reagent Lines Reconnect the modules using Individual Reagent Lines provided in your startup kit The assembled manifolds may be used if they were in use prior to the move Connect Individual Reagent Lines 1 Match the corresponding labeled reagent line on the module with the line from the Individual Reagent Lines kit Tightly twist the two connectors together Repeat for all eight lines 68 Place the reagent lines in the reagent bottles For additional information on Individual Reagent lines refer to Installing RapidTrace Hardware Reagent Lines option 2 p 6 Reconnect Assembled Manifolds Follow the instructions below to swit
24. here oo e Check valve end To Reagents Figure 11 Reassembling Manifold String 71 Continue placing manifolds on the workstation connecting them to each successive reagent line until all eight reagents have been completed 72 Connect the check valves to the manifold and place them in the appropriate reagent bottle NOTE L Skip the following Gas Lines section if gas drying is not used Gas Lines Locate the following items in the startup kit a 10 foot gas line and 2 types of fittings for each module to connect to a gas tank Connect this line to the gas tank using the appropriate fitting Follow the instructions below to reconnect the line to your modules Reconnect a Gas Manifold Reconnect the manifold to the gas line using the instructions for reconnecting manifolded reagents NOTE H The 1 8 in 3 175 mm tubing is for the gas line the 1 16 in 1 59 mm tubing is for reagents P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 68 Adding or Moving RapidTrace Modules Connect an Individual Gas Line Attach the individual gas fitting found in the startup kit between the line to the gas tank and the line to the module as shown in Figure 12 Attach lines here Line to Module Line to Gas Tank Individual gas fitting Figu
25. is being used turn off the gas at the gas tank first then disconnect the gas line attached to the tank Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Adding or Moving RapidTrace Modules 65 Reagent Lines and Gas Lines Locate the plumbing in the manner described in Identify Reagent Plumbing Setup p 60 There can be up to 9 lines per module e Reagents 1 through 8 e Gas line if there are steps for column drying Untwist each of the reagent line connections located several inches from the back of the module and place the lines aside to later bring to the new location If gas drying is being used remove the gas connection as shown below and put aside to bring to the new location Remove this line Line to Module Line to Gas Tank Figure 8 Gas Line WARNING AA Confirm that gas has been turned OFF before removing the gas connection Waste Lines The four waste lines extend from a cutout at the base of the back of each module Se Cutout for waste lines 7 Figure 9 Cutout for Reagent and Cannula Waste Lines Several inches from the back of the module the lines have a connection to make moving the module easier Locate the fittings and untwist the connection The 10 foot lines will remain in the waste receptacle It is now okay to move the modules
26. rack Column Plunger Any Operation Before performing the first column operation the turret must be moved to a sample position Column Plunger Squash Column Lock the column plunger using the Lock Column Plunger operation or the Turret operation Continue If an error occurs Continue allows you to re start the procedure run at the sample of your choice after correcting the error condition This operation will not work with macros it will only work with procedures run individually A report cannot be generated after using Continue To obtain a report set up the rack and columns to run starting at Sample 1 Edit the Setup Rack screen appropriately and re run the procedure using the Run Monitor screen Gas Turns gas valve ON or OFF listed in minutes Shuttle Collect Moves the shuttle to the assigned designated fraction tube c Before using Shuttle Collect the test tube must be in the assigned position First use Shuttle Waste or Shuttle Wash Before sending it to a fraction position first send the shuttle to Waste or Wash P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 58 Error Recovery Performing Individual Operations Continued Operation Command Function Shuttle Out Sends shuttle out rest position Shuttle Sample If the shut
27. replaced once during the PM Beyond this replacement these parts are available at current list prices and are designed for replacement by the customer See the description of Limited Life Parts below e Damage to Limited Life parts caused by insufficient maintenance or cleaning practices or abnormal applications are not covered under this contract If these situations are diagnosed charges are at current list prices for parts and current service rate structure for labor and travel e Application and chemistry support is not included e Software releases that contain new product features and functionality are offered for sale by Zymark and are not included under this program e This warranty applies only to the original users and delivery locations It is not transferable to other users or locations without pre approval from Zymark Limited Life Parts Limited Life Parts are any parts that are exposed to solvents reagents or samples Such parts include but are not limited to syringes valves seals and fittings These parts are warranted to be functional at the time of installation and are covered for thirty 30 days after installation Limited Life Parts are routinely replaced by Zymark Field Service Engineers during a PM visit or during Zymark Repair Depot servicing Otherwise these parts are available from Zymark at current list prices and are designed for replacement by the customer Equipment Return Policy In servicing situations requir
28. sample 8 mLs 2N NaOH one sample 8 mLs 2N HNO3 2 Remove reagent lines from reagent bottles and run PURGE8 SPE 3 Place all reagent lines into a large beaker of deionized water and run PURGE8 SPE 4 Remove all reagent lines from water beaker and run PURGES8 SPE 5 Place all reagent lines into a large beaker of Methanol and run PURGE8 SPE 6 Remove all reagent lines from the methanol beaker and run PURGE8 SPE 7 Turn all modules OFF Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Glossary Analyte Anion Exchange Bed Size Cartridge Cation Exchange Collect Column Bed Condition Derivatize Elute Fraction Ion Exchange Load Matrix Mixed Mode Mode Glossary 93 The molecule of interest in the sample that is to be isolated An ionic exchange mechanism whereby the functional groups on the sorbent are anionic or negatively charged The mass of packing material in the SPE column The container holding the phase The cartridge may be referred to as Tube or Column This manual uses the term Column An ionic exchange mechanism where the functional groups on the sorbent are Cationic or positively charged The action used to remove analyte s of interest from the SPE column May be done in single or multiple steps May be referred to as Collec
29. sliding it up and away from the screw and pedestal 94 Invert the fuse block so that the two 0 8 Amp fuses are on top Fuge Fuse Block Figure 22 Installing the Fuse Block 95 Confirm that the fuses are seated securely in the fuse block 96 Slide the fuse block back into position and tighten the screw Make sure the two active 0 8 Amp fuses face out Jumper Bar Figure 23 Substituting Fuses 97 Slide the cover fuse block assembly back into the housing Snap the fuse block cover assembly securely into the power entry 98 Plug in the power cord and switch the module ON The module should initialize immediately Ee If RapidTrace does not immediately initialize confirm that the correct fuses have been installed and that the fuses are correctly seated P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 84 Service Customer Support Options Zymark Technical Support Center To obtain technical product support during your warranty period please contact Zymark Technical Support Center at 1 508 435 9761 USA or via the Internet at techsupport zymark com or by fax at 1 508 435 0950 USA The Center provides expert services including telephone product troubleshooting repair instruction service and installation scheduling and replacement part information The hours of operation are
30. that appears in the lower right corner These are your choices for the source defined earlier within the reagent setup screen 27 Click on Methanol MeOH will appear in the box for the Source 28 Click once in the third box second line Output The Output is where you want the reagent to go after it conditions the SPE column Output choices will appear in a secondary screen The Output can be any of the three wastes or a fraction tube Waste names were defined in the Reagent Setup screen Aqueous Organic Chlorinated Fraction 1 Fraction 2 Fraction 3 Fraction 4 Fraction 5 Fraction 6 Fraction 7 29 Click on Organic Its abbreviation will appear in the cell for the Output 30 Click in the fourth box second line Vol Vol is the amount of source in mL you want to use to condition the SPE column Type 5 in this box 31 Click in the fifth box second line mL min The number entered into this field will be the flow rate of this step Type 5 in the cell Methanol will flow onto the column at 5 mL min NOTE L When writing procedures the files 1ml spe and 3ml spe can be opened in Create Procedures and used as templates These procedures provide suggestions on a starting point for flow rates Use the 1ml spe template if you are using a 1mL column plunger Use 3ml spe as a template for use with a 3mL column plunger 32 The procedure should now appear as follows Procedure N
31. the files on this disk into the spe directory Place the second disk RapidTrace Software Help Screens into your disk drive and copy the files into the spe directory In File Manager create a directory called rload Place the firmware disk in your disk drive and copy the files on this disk into the rload directory You can now create your icons From Program manager create a program group and fill in the description as follows Program Group Properties Description RapidTrace Lo Group File P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 8 Installing RapidTrace Software Production Icon Create a program item within the RapidTrace program group and fill in the information as follows gt Program ltem Properties Description Command Line Working Directory esse sid o Run Minimized Development Icon Description Command Line Working Directory Shortcut Key Magnetically Encoded Rack Icon Description Command Line Working Directory Shortcut Key CI Run Minimized Firmware Icon Create a program item within the RapidTrace program group and fill in the information as follows Description Zymark C Bun Minimized Click on Change Icon and choose the zymark ico icon Zymark Corporation RapidTrace SPE Workstation Installat
32. to draw your attention to important information and alert you to the presence of hazards as indicated Some of these symbols may not appear in this manual or on the product this manual describes SHE Oo A A Oa M DANGER An imminently hazardous situation which if not avoided will result in death or serious injury WARNING A potentially hazardous situation which if not avoided could result in death or serious injury NOTE A cautionary statement an operating tip or maintenance suggestion which may result in instrument damage if not followed Hazardous voltage risk of shock injury Risk of body parts hair jewelry or clothing getting caught in a moving part Risk of puncture injury Risk of eye injury Wear safety glasses Risk of fire Risk of poison Risk of explosion Hazardous fumes Hot surface risk of burns Protective ground symbol CE compliance mark Signifies that the unit has passed safety tests for grounding high voltage spikes and voltage leakage Equipment Red Stop Yellow Caution risk of danger labels Blue Mandatory action Green Safe condition or information Ee A helpful hint or additional information Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Table of Contents vii Table of Contents Unpacking and Site Preparation
33. wash position and tubes must be in rack Twelve Port Valve Turns valve position to a different assignment This command functions with the shuttle in any position Version Displays the software version in the Reply Message field Rack Queries the workstation to determine how many magnets sample tubes and fractions are present in the rack The hexidecimal Reply Message xxxx xxxx xxxx indicates Magnets Present Samples Present Fractions Present Example 0000 O3FF O3FF Meaning 0 magnets 10 sample tubes 10 fraction tubes Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Error Recovery 59 Operation Command Function Warm Start Reinitializes the RapidTrace workstation A warm start is almost the same as switching the power button off and on A warm start moves the unit to a ready to run condition During a warm start the RapidTrace unit empties the syringe empties the mixing chamber and moves the rack to its out position After a self check the RapidTrace is fully operational and ready for you to run samples perform maintenance procedures or initiate a system shutdown Using Macro Routines A macro is a series of commands that are grouped together under a single command making running tasks quicker and easier When using a macro routine only one module at
34. 0 foot gas line to the gas tank using the appropriate fitting 2 Attach the Individual gas fitting between the line to the gas tank and the line to the module Figure 16 Attach lines here Line to Gas Tank Line to Module Individual gas fitting Figure 16 Connecting Gas Lines P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 72 Adding or Moving RapidTrace Modules Waste Lines The four waste lines extend from a cutout at the base of the back of each module 1 Locate the Waste Line kit within the startup kit These lines are 10 feet long and are labeled 1 through 4 2 Attach each waste line to its corresponding numbered line on the module 3 Place each waste line in the appropriate waste receptacle 4 Check the reagent setup screen for the designation of Wastes 1 2 and 3 There are colored labels in the startup kit to help identify the lines SS ff Cutout for waste lines Figure 17 Cutout for Reagent and Cannula Waste Lines Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Adding or Moving RapidTrace Modules 73 Operations After Moving or Adding Additional Modules After moving the unit or adding additional modules you need to r
35. 2i 4s lt scesscaccets vactaziaaianchsveids inavtiauedan EEE set atin Eea EAE nie Neil ee ede OO 66 Adding a Module to the Workstation cscssscssescsseescesecseesecsevscceaeeccssecaesecaeseecneeseceaeeeesaecaeeaeenevaeeeaseneeaecateaes 69 Operations After Moving or Adding Additional Modules 0 ccccccccessssescessceecesecenecaeecaeeeseeeseseeeeseeeaeeeseeeasenaeenaes 73 P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com viii Table of Contents DOEVICE E 75 Gas Dryme OMVers 1 OM 4 5 2523 sseedeses ws reae e Ea E a ERa Feee EE a atid E EENE EEEE 75 Unblocking a Syringe Discharge Tube ccecccccsccssecssecsseeseeeseeseeeecesecnseceaeceaeceaeceecaecaaecaeecseecaeeeeeneenseneenaeenaes 76 Reloading RapidTrace Firmware 0 ccescsseceseeeceseceeesecseesecuevecenaeenceaecaeesecseeseceasenesaecaeenecaeseeenecaeseesnesreeaeeneeaeeates 77 Troubleshooting scoscese ee REE EE EER EEE EEEE EEE EIER ERE E a EERE 78 I TRAPER E ARN E PEREAT E A E A E A NETA AEA A E T 81 Customer Support Options ccccccccssecsseeseeeseesseeeceseceseceaecsuecsaecseeeseceaeeseeesecesecaeceecaaecaaeesseceseseeeseeeseenseeeseenaeenaes 84 Reference Data a EAE 86 Sp cifications E esses estes glazed scien R E mica Geek eects R R ame ad reas add etiam eater eos 86 Columm Bed Generallyiiccsc zi cn eile es aha cs e
36. 78 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Adding or Moving RapidTrace Modules 61 Adding or Moving RapidTrace Modules Overview You can move or relocate the RapidTrace workstation To make re installation easier review the module s plumbing prior to disassembly so you can put it back together There are colored labels in the startup kit to help with line identification NOTES L e Only qualified personnel should move a RapidTrace module e Before moving the module confirm that power has been switched OFF and that the power cord and the RS232 communications cables have been unplugged Identify Reagent Plumbing Setup There are two module setups e Manifold Setup Reagent lines between modules are connected The workstation uses the same set of reagent bottles with one line that draws up reagent through a check valve e Individual Reagent Lines Each module has eight reagent lines leading directly from the module to the reagent bottle 1 Determine which plumbing setup the module is employing On the back of the module there is a cutout for the reagent lines 1 16 diameter tubing and the gas line 1 8 diameter tubing Cutout for 8 s reagent lines and 1 gas line A an a Figure 4 Cutout for Reagent Lines and Gas Lines 2 Ifyou have manifold pieces shown in Figure 5 connected to your reagent lines you should follow th
37. Cannula 6 30 No g No 80 Inthe Setup Racks screen assign the procedure to Samples 1 and 2 For Modules with Individual Reagent Lines 1 Write a procedure to purge the reagent lines taking the miscibility of the reagents into account For eight reagents an example of a procedure is as follows Create procedure using reagent names for all modules ol x File Edit Reagents Setup Variables Password Step Source Output Vol ml min Sense IPurge Cannula Reag1 Cannula No a Eesthoatiianigg 21 98 11 33 37 AM IPurge Cannula Cannula Run Time 6 22 Purge Cannula Reag3 Cannula Purge Cannula Reag4 Cannula Frocan Tzee Purge Cannula Reege Cannula This procedure purges all eight reagent lines Purge Cannula Reag6 Cannula Purge Cannula Reag Cannula JEJEJ Purge Cannula Reag8 Cannula oan onau N 81 Inthe Setup Racks screen assign the procedure to Samples 1 and 2 P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 74 Adding or Moving RapidTrace Modules Ee Waste Test While the test is running e Check that there are no leaks in the fluid path e Check that air is not being pulled in once the lines are primed e The lines should be full
38. Center hours of operation are Monday through Friday from 8 00 a m to 8 00 p m EST The Center provides expert services including product troubleshooting repair instruction service and installation scheduling and replacement part information Visit our World Wide Web site on the Internet at www zymark com P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com iv Preface For support in Europe contact Zymark LTD Runcorn UK 44 1928 711448 or fax 44 1928 791228 For more information contact your local Zymark representative Copyrig ht Copyright 2000 Zymark Corporation All rights reserved Reproduction in whole or part is prohibited Trademarks RapidTrace is a trademark and the Zymark logo is a registered trademark of Zymark Corporation All other trademarks and registered trademarks are the property of their respective holders Contents This guide provides the information you need for using the RapidTrace workstation The information in this manual may contain typographical errors or technical inaccuracies and is subject to change without notice Modifications may also be made to the product described in this guide at any time For the most up to date information consult the on line Help screens FCC U S A This equipment has been tested and found to comply with the limits for a Class A d
39. Number ml min 1 Water H20 30 2 Methanol MeOH 30 3 Acetone Aceton 30 4 Isopropanol Isopro 30 5 Toluene Tol 30 6 Hexane Hex 30 7 Tetrahydrofuran THF 30 8 Ethanol EtOH 30 ra aste Waste Name Abbreviaton 1 Aqueous Waste Aqu W A Organic Waste Org W 3 Chlorinated Waste Chlorw Column tubing volume 4 default entries OK Save as current Help P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 24 RapidTrace Software Assigning Reagent Setups to Individual Modules A Reagent Setup file is initially set for one module however once the screen is saved as a set file it can be assigned to any module The actions below illustrate this point In this sample Reagent Setup Screen is set for an Individual Module and made active for use by clicking on the OK Save as Current button Upon returning to the Reagent Setup screen from the Main screen the Reagent Setup screen will look like it is active all the other modules But the default screen IS NOT active UNLESS you click on the OK Save as Current button for each module individually Unless each module has been setup individually the Reagent Setup screen will show the last values saved for that module Refer to pages 22 and 23 for more information on set files 1 From the Main screen click on the Reagent
40. PE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 90 Reference Data Accessories and Kits For ordering information please refer to Technical Support in the Preface of this manual Part Number Description 60969 O ring for the 1mL Plunger Qty 1 45096 O ring for the 3mL Plunger 50973 Tubing Weight Kit Free of Charge 1 kit per module Contains 8 weights 50974 Standard Rack Standard rack accommodates 13x100 mm sample tubes and 12x 75 mm fraction tubes Standard Rack Set of 5 Standard rack accommodates 13x100 mm sample tubes and 12x 75 mm fraction tubes 52229 1mLColumn Plunger J oo 52231 3mL Column Plunger o O 52232 Camnula Replacement Kit o 52234 52689 RapidTrace Notebook Contact the Zymark Technical Support Controller Center 54405 Contains 10 magnets for encoding racks 55076 Check Valve Kit Contains 8 check valves and lines 55091 11mm Vial Adapters Contains 10 vial adapters for conversion of a Standard Rack 55827 4 mL Vial Adapters Contains 10 adapters to convert vials to standard rack 56536 Chilled Rack 9 position For the same size test tubes as Standard Rack 56540 RapidTrace Desktop Controller 56786 16 x 100 mm Rack For 16 x 100 mm test tubes on both the sample and fraction sides 57144 Spare fittings and tubing kit ooo O 57145 1 mL blank columns and Contains 10 each sp
41. Setup Variables Password Procedure Sip Speeds Step ml min Mixer 30 Sample 15 Constants Column air push volume 2 Column air push speed multiplier 2 oN moO ewn MNN 10 11 12 ok me Password Procedures and reagent setups can be password protected The password may be any 128 character string of printable keyboard characters Do not forget your password Type in new password TE OK Cancel NOTE H If you forget your password call Zymark Technical Support Center For more information refer to the Technical Support section at the Preface of this manual Procedure Name The procedure name is a DOS format file name Up to eight characters plus the spe extension can be used The software automatically attaches the spe extension to a procedure name Created When a new file is opened in Create Procedures the software will automatically access the time and date from the computer s operating system and place that data in the box next to the words Created The date and time are not changeable fields Last Modified When an existing procedure is changed the software will automatically access the time and date from the computer s operating system and place that data in the box next to the words Last Modified The date and time are not changeable fields Run Time An estimated time to complete the p
42. Setup button Click on Module Number located under the title bar 2 Click on the circle for Module 0 and Click OK Notice the title bar changes to say Reagent setup for module number 0 Reagent setup for all m File ModuleNumber C Module 0 C Module 1 Module 2 C Module 3 C Module 4 C Module 5 C Module 6 C Module 7 C Module 8 C Module 9 All Modules OK w Reagent setup for module number 0 File ModuleNumber r 3 Click on the default entries button to bring back the default entries 10 Click on Module Number again click on the circle for Module 1 and click OK Notice the title bar changes to say Reagent setup for module number 1 11 Click on the default entries button All of the default entries will appear Repeat this process to assign the default entries for Modules 2 3 4 and 5 12 Click on the OK Save As Current button The default entries have now been assigned to Modules 0 through 5 The test1 set setup is still assigned to modules 6 through 9 13 Click on Reagent Setup Click on Module Number Select All Modules then click on OK Notice that the test1 set data appears in the fields Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ee 14 15 16 RapidTrace Software 25 Note that although the information appears i
43. The step s used to flush the column with reagent after the sample is applied to remove unwanted species May also be called rinse Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Index A ACCESSOMES s cc cdccsieisacctevinnceadeiactetscdedeess visis rs rossi ose riss 85 Adding a manifold ssseeseeeeessssreresesrrsereesrserssen 70 Adding a moduler se siniiiiisisesieiesiesrs 69 Adding modules ccccesccscecseeneeeeeeeseeereeeeeeeeesaees 61 Audiente arenen EEEE nucetavtatecsalenseetevireeess iii B Beep Control ccceccesecesecseeceeceeeeeeeceeeeeeeenseeneeaees 42 Beni chs pace sicsceschis ceacianin ances EEE 2 C Cables COMMUNICATIONS ccecccceessececeescececeeseeceesseeeeesaes 3 communications Cable cccceccsccceesseceesseeeeees 15 Cannula purging and cleaning eeceeeeseeeteeseeteeeeeees 34 Check ValNera r eens 6 Column bed i s205 esti cence ans 87 93 COMM POM fecccssaccecnssSececcetieveervacedebsag sstenesns EEEN 10 Changing 2 cs scesccsarceseccadessevandieses oine a 18 fear ES E O 18 Computer requirements ssssssessseeeesee seser sees se seese 7 Creating procedures eseeeeerererersrrrrerereerersrre 26 Customer ID code sssssnoosseoeeeeeseseesseseesseresesresseseesseee iii Customer support plans ccccecceeese
44. Variables Password Biel Es Created Purge Cannula Reag1 Cannula 6 Last Modified Purge Cannula Reag2 Cannula 6 y 6 22 Purge Cannula Reag3 Cannula 6 ean ae Purge Cannula Reag4 Cannula 6 Frocedure Descnotion Porge Cannula Reao5 Cannla 6 30 Wo Purge Cannula H20 Cannula 6 30 No Punge Canncla Reso Cannulaj a0 Wo Ponge Canndla Reag Cannulaj 30 Wo This procedure purges all eight reagent lines 73 Inthe Setup Racks screen assign the procedure to one sample CON DO ewe N 74 Run the procedure for all modules in the workstation A test tube must be placed in Sample position 1 on the rack 75 When the modules complete the procedure check the lines for fluid Attach the Manifold Piece 1 Locate the manifold pieces and plugs in the startup kit Remove two manifold pieces 2 Insert the Luer fitting on the end of one manifold piece onto the T connector of the next piece Figure 14 and twist until the two manifold pieces are connected snugly oe Vey fT a Figure 14 Connecting Two Manifold Pieces Together 76 Work on one manifold string at a time as each string is for a different reagent Lay the new manifold piece for Reagent 1 along the back of the new module so the tubing end faces its adjacent module
45. a time can be run unless the number 10 is placed in the module number at the lower center of the screen A 10 allows all modules to be run under the same macro routine NOTE H There is no error checking in the macro mode of operation Example A macro is run that has been written to perform operations on two samples After processing sample 1 the computer finds that a test tube is not in position 2 on the rack The computer will hang cease to function and need to be rebooted Create and Save a Macro If an incorrect message is sent during the creation of a macro routine the entire routine macro will need to be rewritten Follow these steps to create and save a macro 1 Inthe Error Recovery screen select an operation from the box on the left side of the screen by highlighting it Some selections on the left side of the recovery screen have sub selections that will appear in a box in the top center of the screen 2 Click on the Send button The module selected will perform the operation The selected operation will be displayed to the top right box of the screen All of the operations that appear in this box at the top right of the screen will be included in the macro 3 Continue to repeat steps 1 and 2 for each operation that you wish to include in the macro P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guarant
46. ace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Software 41 44 45 46 47 48 49 Select Samples 1 through 5 by clicking once on Sample 1 to highlight it hold down the Shift key and then click on Sample 5 Sample1 through 5 will be highlighted Click on the arrow button between the two boxes The test 2 spe procedure will now appear assigned to Samples 1 through 5 Click on the 1m1 spe procedure Click on Samples 6 through 10 by highlighting and dragging with the mouse as in step 7 Click on the arrow button between the two boxes The 1m1 spe procedure is now assigned to Samples 6 through 10 Click on the OK Save button in the lower right corner of the screen This will save the setup and return you to the Main menu Setup Procedure Sequence Of x Procedures on file Procedures assigned to Module 0 Sample 1 test2 spe l Sample 2 test2 spe Sample 3 test2 spe Sample 4 test2 spe Sample 5 test2 spe Sample 6 Iml spe Sample 7 1ml spe Sample 8 1ml spe Sample 9 1ml spe Sample 10 1ml spe Delete selected item M Module0 P Module 5 Selected Procedure 5 Module1 X Module 6 Description Module 2 Module 7 Module 3 X Module 8 Module 4 Module 9 Select All Clear OK Save Help P N 55078 Rev 5 RapidTrace SPE Workstation
47. acers 58262 Gas conversion Lines 58728 __ 3 mL Disk Adapter po 58759 1 mL Disk Adapter Ooo 65158 RapidTrace Procedure Checker This includes manual and disks Order Kit All part numbers are subject to change without notice Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Reference Data 91 Extended Inactivity Preparation Occasionally the RapidTrace workstation may be taken out of service for a prolonged period perhaps during vacation times lighter sample loads etc It is however very important to prepare the modules for downtime To prevent salting out of buffers and corrosion from any acid used rinse the reagent lines with water Removing the standard reagent set and rinsing with water is essential NOTES e Do not allow water to sit in the fluid path and valve with no movement for an extended period of time i e approximately ten days or more e Standing water in the lines can cause bacteria growth especially in warm humid environments Rust may form in the brazed fittings of the 12 port valve If a RapidTrace will not be used for an extended period of time i e approximately ten days or more it must be kept clean and dry Be sure that the last procedure run on all modules is the cleanup routine Purge all of the standard reagents from the lines with a pr
48. alling and running a macro routine Recall Saved Routine Run Displayed Routine See Using Macro Routines p 58 for more information Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Error Recovery 57 Performing Individual Operations To perform an individual operation on a module 1 choose the module number 2 select the operation and any additional choices that are necessary for that operation and 3 click the send button Each operation or command and their function are described below Operation Command Cannula Function Three options are available with this command Cannula Up Moves the cannula up to the home position that is out of the sample test tube Into Wash Moves the cannula into the wash stations Into Sample Moves the cannula into sample Rack must be in appropriate position with test tubes in place Confirm that the cannula is in an allowable list position legal position before sending the cannula down Column Plunger The shuttle must be in waste or wash position Three options are available with this command Up column plunger up in rest position Lock Turret Plunger about halfway into column Squash Column moves column onto the top of the SPE bed The SPE column must be in place and test tubes must be in the
49. ame test2 Step Source Output Yol ml min Sense 1 Add to Sample H20 Cannula 5 5 g 5 5 2 Condition Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Software 33 Load Sample onto Column 1 Click once in the Step box for the third row This will be the third step in the procedure Procedural step options will appear in the secondary screen in the lower right area of the Main screen 2 Inthe secondary screen click on Load Sample onto Column This step will appear in the box and Sample will appear as a default under the adjacent Source column 3 In the upper right corner a field will appear for entering the cannula depth in mm from the bottom of the test tube This value should be changed if you do not wish the cannula to go to the bottom of the test tube For this exercise leave the value set at 0 Cannula Depth in mm from bottom o 4 Click in the Output box for the third row The output is where the sample will go after it is loaded on the SPE column Output choices will appear in the secondary screen lower right corner of the Main screen The Output can be any of the three wastes or a fraction test tube 33 Click once on Aqueous The abbreviation will appear in the cell for the Output 34 Click in the Vol box for the third ro
50. anty period Zymark will not be held liable for a missed PM due to restricted access to the equipment during the coverage period e When Zymark provides telephone fax or modem support the customer is responsible for completing any necessary documentation of the service e Ifthe customer maintains a change control validation logbook as a permanent record then he she is responsible for entering all service documentation into this log e Customers must perform the appropriate level of revalidation required as a result of the maintenance or service provided Exclusions Services that alter the hardware or software on Zymark products covered under this warranty that are performed by a vendor other than Zymark Corporation or an approved Zymark vendor will void this warranty e Many Zymark products require the use of Zymark Automation Certified Disposables for proper operation These may include but are not limited to pipet tips seals labels and filters Use of this product with any disposables other than the specified Zymark Automation Certified Disposables will void this warranty e This warranty is intended to cover equipment manufactured by Zymark Equipment purchased from other vendors is not covered by this warranty P N 71466 Rev 2 Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com e Limited Life parts are covered for thirty 30 days after installation and are
51. anual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Zymark Corporation 6 Installing RapidTrace Hardware Reagent Lines Before the modules are installed decide the plumbing arrangement for the reagent lines There are two options 1 Manifold Setup Use this setup when you want to use the same reagents for all modules in the workstation Each unit is plumbed together with a manifold and has a Check Valve that goes into the reagent bottle The Check Valve prevents reagent from flowing out of the reagent line should you remove a module from the workstation Reagent bottles should have a stopper to prevent contamination and to help keep the check valve at the bottom of the bottle Individual Reagent Lines Use this setup when you want to use different reagents for each module Kits of tubing weights may be ordered free of charge P N 50973 one kit per RapidTrace module to keep the reagent lines at the bottom of the reagent bottles Each tubing weight kit comes with eight 8 weights Please see Technical Support in the Preface of this manual for information on how to contact Zymark WARNING MN A A Liquid exiting the solvent and sample lines can be toxic flammable and or corrosive Refer to the MSDS Material Safety Data Sheet for detailed information regarding chemicals used in your applications Do not touch waste Keep waste from heat or flame Dispose of waste proper
52. apidT race Development Magnetically Encoded Rack Load RapidTrace Loads firmware into the RapidTrace module Firmware Icon Load RapidTrace Firmware P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 10 RapidTrace Software Overview NOTE RapidTrace software does not contain volume checking for exceeding the volumes of your sample test tubes fraction tubes and mixing chamber Zymark Procedure Checker software will check procedure files for errors and will suggest improvements This software P N 65158 is loaded separately from the RapidTrace software Accessing the Software To access a RapidTrace application double click on the Production Development or Magnetic Rack icon in your Windows RapidTrace application group NOTE i To use the RapidTrace Production icon a procedure needs to be created using the Development software and run at least ONCE before you can use the Production software This is necessary so that the reagent setup screen information can be loaded to the modules Loading the Firmware Firmware can only be loaded on one module at a time Prior to loading the module must be switched on attached to the Controller and addressed as Module 0 ONLY ONE MODULE AT A TIME CAN BE DESIGNATED AS MODULE 0 The modules can remain connected but all modules must be swit
53. ardware Installing RapidTrace Hardware In most cases a Zymark Representative will install the RapidTrace hardware During installation the Zymark Representative will unpack setup and connect the Controller to the RapidTrace SPE Workstation up to ten modules The Representative will run diagnostics to confirm the operation of the hardware To add a module refer to Adding a Module to the Workstation p 69 For assistance with installation call the Zymark Technical Support Center Refer to Technical Support in the Preface of this manual Tool Needed Small flat blade screwdriver Initialization Initialization is a module self test that occurs every time it is switched on The self test checks that all components of the RapidTrace are in the correct position to start running samples and it confirms that the RapidTrace itself is properly functioning If an error is encountered during initialization the error light will illuminate For initialization the RapidTrace modules require that a rack be placed into the shuttle The RapidTrace Controller does not need to be switched ON and no test tubes or SPE columns are necessary When the power is switched ON or a Warm Start command is sent from Error Recovery the RapidTrace modules go through the Initialization process When RapidTrace SPE Workstation initializes listen to the module s to assure that the shuttle is moving smoothly and check that the error light does not
54. ce SPE Workstation is cooled by the exhaust fan located in the rear of the unit The RapidTrace SPE Workstation is grounded through the three pronged power cord and through the grounding terminal in the power entry module located in the rear of the unit The RapidTrace SPE Workstation should be mounted only in a horizontal position on a flat sturdy surface The RapidTrace is capable of running any SPE column manufactured in the industry standard 1mL or 3mL syringe barrel format within certain bed height requirements The bed height of the sorbent needs to be between 0 4 cm and 4 0 cm high To measure the bed height stand column on flat surface and measure from flat surface to top of frit in the column If the bed height is between 0 4 cm and 4 0 cm the RapidTrace will run the SPE column P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 88 Reference Data Solvent Compatibility Chart The RapidTrace fluid path contains Teflon Stainless Steel and Polypropylene Refer to the chart below for reagent compatibility with these materials Solvent Teflon Lines Stainless Steel Polypropylene Water YES YES YES NaOH to 2N YES YES YES HNO to 2N YES Rinse with YES water after use HCl YES See note 2 YES NH OH YES YES YES Aqueous Buffer Rinse lines with YES YES Solutions water before using or
55. ch manifolds from a four module workstation to two workstations each composed of two modules ce Skip to Step 2 if the entire workstation has already been moved 1 Modify the manifold string for two separate workstations by disconnecting the string at the length appropriate for the new workstation See Figure 10 Untwist here and add Check Untwist here Valve line from and add plug Kit 59076 fie y fie og V pd PV ope Check valve end Plug To Reagents Figure 10 Disconnecting Manifold String Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Adding or Moving RapidTrace Modules 67 Reconnect Assembled Manifolds Continued 69 Toreconnect the manifolds work on one manifold string at a time as each string is for a different reagent Lay the manifold for reagent one on the back of the workstation with the check valve end closest to the reagent bottles 70 If plugs are attached remove them and connect the line for Reagent One from each module in the workstation to the manifold Labels in the startup kit can be used to help identify lines For example if you have a two module workstation connect the lines as shown in Figure 11 Reag 1 From Module 1 Reag 1 From Module 2 Remove plugs if there are any Re and Connect lines
56. ched on Ifa Comm Port other than Comm 1 is being used call the Zymark Technical Support Center for information on how to edit files prior to loading For assistance refer to the Technical Support section at the Preface of this manual ee For instructions on reloading the Firmware refer to Reloading RapidTrace Firmware p 77 Once the module to be loaded is set to module 0 double click on the Load RapidTrace Firmware icon Load RapidTrace Firmware Watch the module as it is being loaded Confirm that the Power Run and Error lights turn ON indicating that loading is occurring The controller displays a whirlybird the cursor will appear to spin When the whirlybird stops check that the module initializes If any of these steps do not perform check your setup and double click on the icon again Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Help RapidTrace Software Overview 11 On line Help can be accessed at anytime within the RapidTrace software by clicking on the Help button in the lower right corner of each screen Help The top of the Help screen looks like the picture below amp RapidTrace File Edit Bookmark Options Help cone Sem ow lt gt To learn about how to use Help push the F1 key which opens the How to Use Help scree
57. cing will be performed e When the equipment and or the environment are determined to be hazardous by Zymark personnel Zymark reserves the right to refuse servicing the equipment One of two situations will then occur 1 The customer will decontaminate the equipment and or the environment in which the equipment is located to the satisfaction of Zymark personnel prior to Zymark servicing 2 The customer decides not to decontaminate the equipment and or the environment and take responsibility to service the equipment themselves through on site verbal instruction from Zymark personnel Parts Availability and On Going Support Zymark provides support for its products for a period of five 5 years after they are no longer available for purchase No Other Warranties ZY MARK SPECIFICALLY DISCLAIMS ANY AND ALL OTHER PROMISES REPRESENTATIONS AND WARRANTIES EXPRESSED OR IMPLIED THIS INCLUDES BUT IS NOT LIMITED TO THE PRODUCT S CONDITION ITS CONFORMITY TO ANY DESCRIPTION OR REPRESENTATION THE EXISTENCE OF ANY LATENT OR PATENT DEFECTS IMPLIED WARRANTIES OR MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE IN NO EVENT WILL ZYMARK BE LIABLE FOR ANY LOSS OF PROFIT OR ANY OTHER COMMERCIAL DAMAGES INCLUDING BUT NOT LIMITED TO SPECIAL INCIDENTAL CONSEQUENTIAL OR OTHER Zymark Corporation P N 71466 Rev 2 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Preface iii Overvi
58. e Abbreviaton Waste 1 Wastel Waste 2 Waste2 Waste 3 Waste3 Column tubing volume 4 OK Save as current Help Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Software 21 Defining Variables for the Reagents The following procedure demonstrates how to define the variables in the Reagent Setup screen 1 Click on Reagent Setup from the Main menu 2 Click on the default entries button in the lower left corner The default entries will be displayed in the Reagent Setup screen 3 Return to the Main menu by clicking on the OK Save As Current button 4 Click on Reagent Setup 5 Place the cursor in the Reagent Name field Highlight Reagent 1 located under Reagent Name Type in Water 6 Highlight the word Reag1 located under Abbreviation Type in H20 7 Continue filling out the information in the screen as shown in the figure below te Reagent setup for all modules BEE File ModuleNumber Renee Reagent Name Abbreviation We H 1 Water H20 fo 2 Methanol MeOH j30 3 Acetone Aceton 30 4 llsopropanol Isopro 30 5 Toluene Tol 3 6 Hexane Hex 30 7 Tetrahydrofuran THF 30 8 Ethanol EtOH 30 x awe Waste Name Abbreviaton 1 Aqueous Waste Aqu W 2 Organic Wast
59. e Org W 3 Chlorinated Waste Chlorw Column tubing volume 4 default entries OK Save as current Help NOTE L The minimum sip rates are 0 36mL min and 0 006 mL s P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 22 RapidTrace Software Saving the Reagent Setup Screen as a Set File The information that you entered in the Reagent Setup screen can be saved as a data file for later use The procedure here demonstrates saving the data that you entered as a set file Save As x File name Folders testi c spe Cancel Network M Read only Save file as type Drives Setups set x amp c windows95 x 1 Click on the word File located under the title bar 2 Choose Save As and type test1 in the File Name box The software automatically attaches the set extension to the file name The limit for file names is eight characters plus the three character extension 3 Click on OK to return to the Reagent Setup screen Ee Name the files so you can easily find them Consider naming the file to reflect the function of the procedure or the class of the agents for example Drugs of Abuse procedures might be named the doa set NOTES e The reagent setup file name is a DOS format name that allows up to eig
60. e block assembly back into the power entry port housing and snap it into place 92 Plug in the power cord Switch the module ON The module should initialize immediately ce Ifthe RapidTrace does not immediately initialize confirm that the correct fuses have been installed and that the fuses are correctly seated Converting the Fuse Configuration To convert the unit from the single 1 5 Amp fuse configuration to the dual 0 8 Amp fuse configuration refer to the diagrams and instructions below DANGER OA Electrical shock hazard Switch the power OFF and disconnect the power supply before changing the fuses For continued fire protection and correct functioning of the unit replace fuses only with fuses of the same type and rating Tools Needed small flat blade screwdriver small Phillips screwdriver 1 Switch the RapidTrace module s power OFF and unplug the power cord 2 Using a small flatblade screwdriver gently pry the cover fuse block assembly away from its housing and remove Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Service 83 re Power Entry Port Housing g aan LaF Cover Fuse Block Assembly _ wee O Fuse Figure 21 Removing Cover Fuse Block Assembly 93 Loosen the fuse block Phillips head screw by two turns Remove the fuse block by
61. e collected two fractions the procedure would be assigned to Samples 1 3 5 7 and 9 Five sample test tubes would be placed in these positions in the rack five SPE columns in these positions on the turret and 10 fraction tubes in the rack tj Setup Procedure Sequence of x Procedures on file Procedures assigned to Module 0 and selected group koolaid2 spe gus spe hicprime spe idisk1 spe koolaid2 spe koolaid2 spe koolaid2 spe koolaid2 spe Delete selected item PX Module 0 P Module 5 Selected Procedure IX Module 1 X Module 6 Description X Module 2 X Module 7 class experiment x Module 3 x Module 8 X Module 4 Module 9 Select All Clear OK Save Help P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 40 RapidTrace Software Assigning SPE Procedures to Samples in the Workstation The following steps demonstrate the process of assigning a procedure 1 42 43 Open the Setup Racks screen by clicking on the Setup Racks button from the RapidTrace main screen Click on the test 2 spe procedure in the box in the top left corner Refer to the Selected Procedure Description box in the lower right of the screen for a description of the file Click on Sample 1 in the box directly to the right of the Procedures on file box The
62. e if they are e Fresh e Correct for the procedure e Sufficient to run the desired number of samples e On the correct numbered line WARNING AO Avoid eye injury Wear safety glasses when working with reagents Always use extreme care when handling fluids in the laboratory WARNING AAA Liquid exiting the solvent and sample waste lines can be toxic flammable and or corrosive Refer to the MSDS Material Safety Data Sheet for detailed information regarding chemicals used in your applications e Do not touch waste e Keep waste from heat or flame e Dispose of waste properly e Periodically check waste lines for damage Confirm that flow is not inhibited and that the waste container is large enough to hold waste generated Follow these steps to prepare the workstation for running procedures 1 Confirm that the waste reservoirs are empty 2 Purge the reagent lines before running samples see Purge Reagent Line p 73 Do this whenever the workstation has not been used for ten hours or more or if new reagents have been placed on the workstation 3 Confirm that all sample tubes are clean and that the correct number of fraction tubes needed are on hand 4 Confirm that all SPE columns are new and are the correct type for the procedure Is there is a sufficient number of them available to run the samples 5 Perform a Post Run Cleanup at the end of the day p 51 P N 55078 Rev 5 RapidTrace SPE Workstation Instal
63. e instructions for Moving Manifolded Modules Otherwise follow the instructions for Moving Modules with Individual Reagent Lines m CI we Figure 5 Manifold Plumbing Piece The entire workstation or just a part of it can be moved To move a part of the workstation only a check valve kit P N 55076 will be needed This kit will supply P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 62 Adding or Moving RapidTrace Modules tubing and fittings for additional lines to place in your reagent bottles for your second workstation Purge Reagent Lines WARNING A Exercise caution when handling fluids in the laboratory Liquids must be removed from the reagent lines To remove fluid from the lines complete the following steps 1 Remove all the lines from the reagent bottles and place them in an empty container to keep them clean 2 Write a procedure to purge reagent lines taking the miscibility of the reagents into account For eight reagents an example of a procedure is as follows t Create procedure using reagent names for all modules Oy x File Edit Reagents Setup Variables Password Procedure Name PURGEGSPE Source Output Vol mini Purge Cannula Reag1 Cannula 6 Created 478 98 3 53 30 PM Last Modifi
64. e is run Service 79 Solution If magnetic racks have not been configured do not use the Start Stop button on the module Instead start and stop runs by using the buttons in the Run Monitor screen If magnetic racks have been configured re check the procedure assignments and make any necessary changes before re starting Run All button disappears in Run Monitor screen Run All button is only available when all modules are running the same procedure Run All button has been known to disappear in earlier versions of the Rapid Trace software Upgrade to version 1 20 if needed Module will not start after the Run button is pressed Check to ensure that the correct procedure is assigned to the correct module and that all test tubes and SPE columns are in place Go to the Error Recovery screen Send the Warm Start Command to re initialize the module Restart the run from the Run Monitor screen when initialization is complete Device Unavailable message appears or assigned procedures disappear when Run Monitor screen Check and tighten connections between modules and computer Delete and re enter Comm Port number Confirm that the proper Comm Port is assigned under Comm Port menu Switch Comm Ports to rule out port failure initializes Switch all modules and computer OFF wait 10 and restart computer and modules Syringe leaking Remove knurled nuts from bottom of syringe to remove black plate and
65. e owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment 7 EQUIPMENT DEMOS HUNDREDS OF InstraV ea REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED D a gaa tia Contact us 888 88 SOURCE sales artisantg com www artisantg com
66. eans the liquid handling syringe the 12 Port Valve and the cannula CS We recommend that all procedures contain multiple Purge and Clean cannula steps For more information about purge and clean operations refer to Carryover Prevention p 36 Saving the Procedure There are two methods of saving the procedure 1 Click on the word File under the title bar and then click on Save The Save As screen will open showing where the file will be saved and the file name Click on OK and the file will be saved After saving the procedure remain in the Create Procedures screen with the saved procedure open Or Click on the OK Save button located in the lower right corner of the screen The Save As screen will open showing the file name and the save to location Click on OK The file will be saved and the software will return you to the Main menu Save As 21x File name Folders c spe Cancel Iml spe a aci a 3ml spe amp spe clean spe Network r Read only Save file as type Drives Methods spe z c windows95 X Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Software 35 Editing a Procedure Procedures are easily edited using the Create Procedures screen 1 2 37 38 39 40 41 From the main Development sc
67. ect functioning of the unit replace fuses only with fuses of the same type and rating Tools Needed small flat blade screwdriver small Phillips screwdriver 1 Switch the RapidTrace module s power OFF and unplug the power cord 2 Using a small flatblade screwdriver gently pry the cover fuse block assembly away from its housing and remove it to access the fuses 3 Loosen the fuse block Phillips head screw by two turns Remove the fuse block by sliding it up and away from the screw and pedestal re Power Entry Port Housing Cover Fuse Block Assembly _ Sake Figure 19 Removing Cover Fuse Block Assembly P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 82 Service Replacing the Fuses Continued 89 Remove the fuse either the two 0 8A fuses or the single 1 5 Amp fuse and replace with fuses of the same rating Screw as PAK Vo W a j Pedestal 4 Jumper Bar Figure 20 Installing the Fuse Block and Fuses 90 The active fuses will face outwards that is towards you Slide the fuse block back into position and tighten the screw NOTE L For 110 Volt compliance the single 1 5 Amp fuse and jumper bar will face outwards i e towards you for 220 Volt compliance the dual 0 8 Amp fuses will face outwards 91 Slide the cover fus
68. ed 4 8 98 3 55 35 PM Run Time 101 3 Procedure Description This procedure purges all eight reagent lines 61 Inthe Setup Racks screen assign the procedure to Sample 1 Liquid Sense Purge Cannula Reag2 Cannula 6 Purge Cannula Reag4 Cannula 6 Purge Cannula Reag5 Cannula 6 Purge Cannula Reag6 Cannula 6 5 No Reag Cannula Reag8 Cannula wCaOonnonetnn 62 Run the procedure for all modules in the workstation even if some of them are not being moved A test tube must be in Sample position 1 on your rack When the modules complete the procedure check the lines for fluid Disconnect Plumbing When liquid has been removed from the lines disconnect the plumbing WARNING A If gas is being used turn OFF the gas at the gas tank first then disconnect the gas line attached to the tank Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Adding or Moving RapidTrace Modules 63 Reagent Lines and Gas Lines Locate the modules to be moved It is easiest to remove the modules at the end of the workstation furthest from where the reagent lines are placed into the reagent bottles If the entire workstation will be moved it is not necessary to attach the plugs mentioned below in steps and 2 63 64 65
69. eeaeeeeeneeaeeenees 43 View and Print Run Reports snene ien i A E ROE E E A e E ESS 45 Magnetically Encoded Racks sssrin eesse eae eaer AERE E EEE EEES RaRa EEE E reaR TEE E a REEERE aS 47 Daily Operations csccssccssscssscssscsssessssnsssnsssssssesssesssesscsssssssscsescsesssessnssnessnesenesseessesessnessesssessseseseees 51 Daily Operation Checklist ossiani ea snares sstersin E EE EEEE TETEE bebda dex EENETI elses EET EEE RE 51 Cleaning the RapidTrace Modules c ceccesccesceesscssecsecseecseeeneeeeeeseceseeeseceaeceaecnaecaaecaeecaeseaeseaeseeeseeseaeeeaeeeaseeseenaes 53 ETTOr e LUTA ma E E A T E 55 Features of the Error Recovery ScTeet eseccssesseseesessceseeseesecseesecneveceaeeeceaecaessecseseeceaseeeeaecateaecaeseesnasereaeeneeaeeates 55 Performing Individual Operations cesseesscseeeresseeecesecseesecaeesecnavseceaeeeesaecaessecsesseceaeeeesaecaeeaecaeseesnesereeaeeneeaeeates 57 Using Macro Routines cccccccscesssssscesscesecesecescsscessecssecseecaeecaassnecsceesecssecaecaaecsaecsaecsaecaaeeseeeseseaeseeeeeseeeneeseesaeenaes 59 Adding or Moving RapidTrace Modulles scsscccsscsssccsscssscssscnsssssssssssescsesssessscssssssssssessesscsssesses 61 OVEIVIEW n e Peeper eras Tere en ee cer ae Tee eae ae Tne CET eC Eee eR eee eee 61 Moving Modules with Individual Reagent Lines 0 00 00 ceeeceeeeceseeeeesecseesceseceeesecnevseceaeeeceaecaeesecseseeenaseeeeaeeeeeaeeaees 64 Reconmectina Modules ssc
70. eed 888 88 SOURCE www artisantg com 60 Error Recovery Create and Save a Macro Continued 4 5 Click on Save Routine to save the macro A Save screen will appear Name the macro by typing in the filename box using up to eight characters The software will automatically add an err extension which is the default extension in RapidTrace software for routines or macros A macro can be run by clicking on the Recall Saved Routine button and selecting the macro Macros may be used for any module once it has been recalled When running a macro confirm that the correct module is chosen in the Module number field Entering the number 10 in this field causes the macro to run on all modules To clear the macro from the Error Recovery screen click on Clear Routine The RapidTrace software includes two previously written macro templates Recall these routines study what they do and write your own routines for your particular needs Macro Description revblkl err This routine recovers the sample from the fluid path to sample tube 1 Use this macro to recover samples stopped before they are loaded onto the column This routine will save the sample revblk2 err This routine recovers the sample from the fluid path to waste Use this macro to recover samples stopped during or after column loading This routine will not save your sample Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 550
71. eeeeeeeeeeeeeeeneees 84 D Declaration of Conformity cccccccccseesesseeteetees iv V E Error recovery FEITES oi avecesaaaicrs eae cece eee 55 selecting inai E aie a aes 55 F Firmware LOAIING oo eeceeeceeeceeeceeeceeceseceseceeeceaecneeceeceeeaeeeaes 10 reloading oo cece ceseceseeceeceeseceeeceeeeecesecnseeseeceeeaes 77 Flow rate changing UNitSssesrosiiii eiin 19 determining ssor seat oknie eee erst 30 prime reagent lines eee eeeeeeeseeeteceteeeeeeeenees 52 Flow rate conversion chart 0 ccccecsceessseeceseeees 89 Fuse block assembly cccccecceeseeseeeeeeeeeteeeteeeeees 83 FUSES cccccccceececsesseseeecececeesesaeceeececsessaeseeececeeses 81 87 TOPlACING cceececeeseesseeeceeeceeeeeeceeceseessecseeeeeeaes 81 Specifications sessie EEE 3 Index 95 G Gas drying COnversion e seseseesseeeesseerssssersersereee 75 Gas lines COMNECHNG seei e E RE RAS 71 Gas TeQUITEMENES 0 0 0 eeceeeceteeeeeneceeeeceeeercaeeneeaeees 86 G s SUPP y irrien aii iii E e 2 I Internet address ccccecescceseceseceessccesecesseeesseeeeseeees ill L Lin VONAGE receret RRE EREEREER 3 Liquid SENSE ccscceseesceeseeesceeeeeeeeeeeeeeeeneeeseeeeeeaes 30 Liquid sensor VOL we lmit sirsenis 31 Luer fitting e ce ceeeceeceesceeeceeeeeereeeceseenseeneeeaeeaees 70 M Macro routine DULCONS cc ceecceessecsssceeseecssecesseecsseeeeees 56 USING TOULNES 6 0scssecsesicdieecssetsneeed edvecesne
72. eplaced once during the PM See description of Limited Life Parts below e Cosmetic surfaces are warranted for thirty 30 days from date of installation Any defects covered by this warranty shall be corrected by replacing or repairing the affected item at Zymark s option Parts replaced during the warranty period will be covered for the remaining term of the original warranty or for thirty 30 days from time of replacement whichever is longer Such replacement parts may at Zymark s option be new or remanufactured All parts removed from warranted equipment become the property of Zymark Corporation Zymark reserves the right to satisfy its warranty obligations in full by refunding the purchase price minus any service validation or travel charges of any defective product Customer Responsibilities e The equipment must be used under normal installation and applications as described in the User s Guide e The equipment must be maintained as described in the User s Guide e Reasonable precautions should be taken to keep caustic atmospheric conditions chemical spills and biological and radioactive contamination from rendering the equipment irreparable which would void this warranty See the Hazardous Limitation Statement for further details e Zymark Automation Certified Disposables are required for proper operation of all applicable products e The customer is responsible for making the equipment available for a PM during the warr
73. er Use a dedicated analog phone line The RapidTrace Controller has a built in modem to allow for communications between the workstation and Zymark for troubleshooting purposes Test Tubes You need to supply 13 x 100 mm input test tubes and 12 x 75mm output test tubes for use with the standard RapidTrace rack Columns You need to supply 1 mL or 3 mL syringe barrel type SPE columns Each RapidTrace module is set up for 1 mL SPE columns A 3 mL plunger is provided for easy conversion Waste Containers You need to supply the waste containers The RapidTrace has four 4 waste lines You determine the destination of lines 1 2 and 3 Line 4 will contains the results of any purge amp clean cannula operation You should consider a separate reservoir for this line WARNING AOA To avoid eye injury wear safety glasses when working with reagents Always exercise extreme care when handling fluids in the laboratory ce For more information refer to Specifications p 86 Power Item 110 120 VAC model 200 220 VAC model Line voltage 105 132 VAC 198 264 VAC Maximum power 900 VA max 900 VA max Line frequency 50 60 Hz 50 60 Hz Fuses 1 5A MDA quick acting 1 0 8A quick acting 2 P N 37645 P N 39838 P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 4 Installing RapidTrace H
74. er lists the Procedures on file that can be assigned to the rack e The box in the top right corner corresponds to the sample positions in the rack Procedures will be assigned to these samples Procedures must be assigned consecutively beginning at Sample 1 unless there are multiple fractions Refer to Multiple Fractions on the following page for more information e The box in the lower left corner designates the modules that the procedures will run on e The Selected Procedure Description Box in the lower right corner shows the description of the procedure that was entered in Create Procedures The description will be shown when the procedure is selected in the Procedures on File box When you click on the file name test2 spe the description that you entered in the Create Procedures screen appears in the Selected Procedure Description Selected Procedure Description This is the sample procedure written for the RapidTrace manual tutorial Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Software 39 Closing Without Saving Clicking on the Lx button in the upper right corner of the screen will close the screen without saving the setup you have established Multiple Fractions Multiple fractions require that sample positions on the rack be skipped For example if the procedur
75. er module Test Tube Sizes standard rack Dispense volume Load volume Liquid Handling Accuracy Maximum Volume in 13x100 Sample Test Tube Maximum Volume in 12x75 Fraction Test Tube Gas Requirements Compressed Gas Supply Inlet Pressure SPE Flow Rates Mixing Capability Liquid Sensing SPE Columns 50 60 Hz 100 240 VAC 10 27 lbs 12 27 kg 21 5 x 4 x 23 in 54 61 x 10 16 x 58 42 cm Sample test tubes 13 mm x 100 mm Fraction test tubes 12 mm x 75 mm 0 1 mL to 6 0 mL 0 1 mL to 5 8 mL Volumes lt 3 0 mL 0 030 mL Volumes gt 3 0 mL 1 9 mL 5 mL Incoming Gas Supply 40 psi 2 8 bars maximum 10 psi 0 7 bars minimum 40 psi 2 8 bars maximum 0 006 mL sec to 0 7 mL sec 0 36 mL min to 42 mL min Cycle mixing Maximum mixing volume of 5 0 mL in mixer Volumes of 1 2 mL or larger 1 mL and 3 mL syringe barrel columns Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Specifications Continued Connections Heat Output per module Fuses Ventilation Cooling Grounding Mounting Column Bed General Reference Data 87 Refer to RapidTrace Parts and Functions p 12 50 100 watts Input voltage 100 120 VAC 1 5 Amp one fuse P N 37645 Input voltage 220 240 VAC 8 Amp two fuses P N 39838 The RapidTra
76. error will occur and the procedure will stop Directs the liquid flow from the syringe to one of the following ports as specified in your procedure e Reagents through 8 8 different ports e Vent e Cannula e Column plunger e Mixing Vessel Selects a module address of 0 through 9 Lights when the power is on Lights when the module is running Lights when an error occurs Delivers the sample or reagent to the SPE column The Column Plunger will move into the column and place the reagent or sample directly onto the column bed Starts the procedure for a magnetically encoded rack and stops any procedure while it is running Pressing the Start Stop switch while the procedure is running will cause the procedure to stop When using Magnetically Enclosed Racks modules must be connected to the Controller Accesses the sample test tube to add reagent to the sample mix the sample or to draw the sample into the syringe and load it onto the SPE column as written in the procedure Holds the 1 ml and 3 ml syringe barrel columns Holds up to 10 syringe barrel SPE columns Allows access to shuttle to change internal tubing Holds up to ten 13 x 100 mm sample tubes and ten 12 x 75 mm collection tubes Holds the magnets for Magnetically Encoded Racks P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www a
77. ess of preparing the sample not just the RapidTrace procedure For most applications the carryover measured could come from any manual sample preparation steps the RapidTrace SPE procedure any sample evaporation or derivation and or the final sample analysis It is important to determine what each portion of the sample preparation contributes to the total carryover If carryover is detected in the RapidTrace procedure examine the steps used to wash out the fluid path Cleaning the RapidTrace Modules p 53 details the fluid path taken by the sample and reagent as a RapidTrace procedure is performed To prevent carryover between samples we recommend that Purge and Clean Cannula steps be used in the procedure Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Software 37 The purge steps should be placed in three locations of the procedure as shown in the following screen capture Con nonkw ne Step Description First Purge Cannula The first Purge and Clean cannula step is done with the Step 5 in procedure first Rinse reagent used often water or an aqueous buffer after the first Rinse step occurs This allows any sample still present in the syringe after the Load step to be moved onto the SPE column with the Rinse step Second Purge Cannula The second Purge and Clean Cannula
78. evice pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the user manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Canada DOC This apparatus does not exceed the Class A limits for noise emissions set out in the radio interference regulations of the Canadian Department of Communications C This device complies with all CE rules and requirements See the Declaration of Conformity for details NOTE L Changes or modifications to this equipment that are not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Preface v Conventions Convention Example Description Declaration of Italics are typically used for titles of manuals section Conformity titles in this manual and text in notes testl set Courier type is used to indicate a pro
79. ew Audience Preface Congratulations on your purchase of the Zymark RapidTrace SPE Workstation This workstation is a high throughput workstation dedicated to solid phase extraction SPE It is a rugged automated platform for quick development of reliable automated SPE procedures from manual laboratory methods This workstation demonstrates Zymark s commitment to improving laboratory efficiency We are confident the Zymark RapidTrace SPE Workstation will be a welcome addition to your laboratory This manual is for the user of the Zymark RapidTrace SPE Workstation for use in a laboratory environment Product Information Keep your product information readily available should you have to contact Zymark Corporation for any reason Record the product information below Model name of product Serial Number found on the product Your Customer ID Code found on your shipping documents RapidTrace Software Version See information printed on the label of the diskettes containing the RapidTrace software or in the title bar on the Zymark RapidTrace SPE Workstation Window software or in the title line of any RapidTrace SPE Workstation procedure printout Technical Support If you have questions about the RapidTrace SPE Workstation that are not fully answered by this manual please contact the Zymark Technical Support Center Call 508 435 9761 Fax 508 435 0950 E mail techsupport zymark com The
80. f x File ModuleNumber Aree ReagentName Abbreviation apie 1 DI Water H20 15 2 Reagent 2 Reag2 30 3 Reagent 3 Reag3 30 4 Reagent 4 Reag4 30 5 Reagent 5 Reag5 30 6 Reagent 6 Reag6 30 7 Reagent Reag 30 8 Reagent 8 Reag8 30 pasie Waste Name Abbreviaton 1 Aqueous West 2 Organic Wst2 3 Chlorinated Wst3 Column tubing volume default entries OK Save as current Help Reagent Setup Screen for Cleaning Procedure 1 Place two sample test tubes in the first two rack positions 2 Place 8 mL of 2N Sodium Hydroxide NaOH into the first sample test tube and 8 mL of 2N Nitric Acid HNO into the second test tube 3 Place two empty columns with spacers in the positions for Sample 1 and 2 in the turret The empty columns with spacers can be found in the start up kit 4 Using the described reagent setup and cleanup procedure run the method 5 Use the Setup Racks screen to assign the clean spe procedure to sample 1 and 2 Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Error Recovery 55 Error Recovery Features of the Error Recovery Screen To access the Error Recovery screen simply click on Error Recovery from the Main Menu screen The main function of this screen is to operate individual module functions to recover from er
81. from the he gas valve to the 12 port valve Newer valves 8 o cloc Older valves C Attach this line from the gas valve to the column Figure 18 External Plumbing Diagram WARNING AAA Never use hydrogen or other flammable gases Use of inappropriate gas may cause combustion or an explosion 1 Remove both ends of the tubing that connects the 12 port valve to the column plunger See the diagram above and the RapidTrace Parts and Functions p 12 for the physical location of parts on the module 2 Locate the 14 5 in 37 cm line of tubing in the gas conversion kit labeled 3 Attach the tubing end with the compression screw to the gas valve port located at approximately the eight o clock position On older valves connect to the valve s Common C port 3 Attach the end of the tubing without the compression screw to the column by pushing it onto the column plunger 4 Locate the 6 5 in 16 5 cm tubing in the gas conversion kit labeled 1 Attach the tubing end with the compression screw to the gas valve port at approximately a two o clock position On older valves connect to the valve s Normally Open NO port 5 Attach the end without the compression screw to the 12 Port Valve position from which you removed the tubing in step 1 above P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisan
82. g i I es 87 Solvent Compatibility Charts sc csscscesecseesss esenea eae ke aaen EE anaa Ea Iaa Eae Er ATE PAE aE EEA ERR Pie EE E ia EENE 88 Blow Rate Conversion Chart svisan enne ennaa EE ea EE A e ar a aeaa a ia a EiS 89 Accessories and Kits erprmerre riri e EE EA r EE ER E E eed wie E e e EE EE NETE 90 Extended Inactivity PreparatiOn sssini iaie a R REE EEEE RE EER ESERE SERE Eaa 91 GIOSSALY TETT EEE 93 E O E ET 95 Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Table of Contents ix P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Unpacking and Site Preparation 1 Unpacking and Site Preparation Unpack the Modules and Check the Parts Unpack the shipping boxes and verify receipt of all the parts listed below Inspect the parts for any evidence of physical damage NOTE Use care when unpacking the RapidTrace workstation Quantity Item Quantity ordered RapidTrace Modules Quantity ordered RapidTrace Controllers 1 per module Standard Rack P N 50974 1 per order RapidTrace Order Kit P N 52006 This kit includes the two RapidTrace operating
83. ganic solvents MeOH YES YES YES Isopropanol YES YES YES Acetonitrile YES YES Not Recommended see note 3 Acetone YES YES YES Acetic Acid YES YES YES Methylene YES YES Not Recommended Chloride see note 3 Chloroform YES YES Not Recommended see note 3 Ethyl Acetate YES YES YES Hexane YES YES YES Notes 1 Polypropylene is used in the Check Valve housing mixing chamber and wash and waste stations 2 HCl at concentrations greater than 0 1N can attack stainless steel and is not recommended Always rinse the entire fluid path with water immediately after using solutions containing HCl 3 Alternate materials may be available Contact the Zymark Technical Support Center for further information See Contact Us at the front of this manual 4 For best performance always rinse the entire fluid path thoroughly after use 5 For information on compatibility with other solvents contact the Zymark Technical Support Center See Contact Us at the front of this manual Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Reference Data 89 Flow Rate Conversion Chart mL sec mL min 0 006 0 36 0 007 0 42 0 008 0 48 0 009 0 54 0 01 0 6 0 02 1 2 0 03 1 8 0 04 2 4 0 05 3 0 0 06 3 6 0 07 4 2 0 08 4 8 0 09 5 4 0 1 6 0 0 2 12 0 3 18 0 4 24 0 5 30 0 6 36 0 7 42 P N 55078 Rev 5 RapidTrace S
84. gram OK Save as Bold type indicates information displayed on the screen current or a button on the screen WARNING Symbols below a NOTE or WARNING indicate what type of danger that could be present such as high voltage fire explosion etc See the Table of Symbols in this Preface for details Product Safety The RapidTrace SPE Workstation has been designed with safety as a foremost consideration however its use is at the discretion and risk of the operator or laboratory supervisor manager Use the product as described in the RapidTrace SPE Workstation Installation and Quick Reference Guide and the on line Help Statement of Proper Use The RapidTrace SPE Workstation is a high throughput workstation dedicated to solid phase extraction SPE It is a rugged automated platform for quick development of reliable automated SPE procedures from manual laboratory methods WARNING AA To reduce the risk of electric shock do not disassemble the unit There are no user serviceable parts inside Refer repairs to qualified service personnel This product must be used under normal installation use and maintenance conditions as described in this manual and in the on line Help P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com vi Preface Table of Symbols These symbols are intended
85. he Dry Column step is used in a procedure Places reagents or samples on the SPE column bed Creates a mixed reagent when the Add Reagents to Mixing Vessel and Mix Reagents in Mixing Vessel steps are used in a procedure Accesses the sample test tube to add reagents to the sample mix the sample or to draw the sample into the syringe to load it onto the SPE column as written in the procedure P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 18 RapidTrace Software RapidTrace Software Main menu Features Overview Comm Port This section illustrates procedures in writing methods using the RapidTrace software and explains the software s key features Consult the on line Help screens for further software information gt Double click on the Development icon The Main menu will be displayed 3 RapidTrace Development There are two features in the Main menu Comm Port and Flow Rate The first feature is the ability to set the Comm Port communications port NOTE L For the Controller to communicate with the RapidTrace the RS232 communication cable must be attached to the assigned Comm Port on the Controller and to a RapidTrace module Changing the Comm Port 1 Click on the words Comm Port located below the title bar 3 Zymark RapidTrace SPE Wo
86. heading over this box is Procedures assigned to Module 0 Click on the arrow button between the two boxes The test2 spe procedure will now appear assigned to Sample 1 Notice the label for the box in the top right corner of the screen has changed to Procedures assigned to Module 0 and selected group The label changed because the sample has been assigned to Sample 1 for all ten modules Module Sample assignments can be determined by looking at the lower left corner of the screen If an X appears in the box the module has the procedure assigned to the samples If you have less than a ten module workstation it is still acceptable to select all ten modules m Setup Procedure Sequence oO x Procedures on file Procedures assigned to Module 0 and selected group Sample 1 test2 spe Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Delete selected item P Module 0 P Module 5 Selected Procedure Module1 Module 6 Description 1 Module 2 Module 7 This is the sample procedure written for the RapidTrace manual tutorial ik Module 3 k Module 8 Module 4 Module 9 Select All Clear OK Save Help In the Setup Procedures Sequence screen click on Sample 1 in the Procedures assigned to box Click on the Delete Selected Item button below the box The procedure has now been removed from the Sample Click on the test2 spe procedure Zymark Corporation RapidTr
87. hipment What is Included e Unlimited emergency on site repair services parts and software updates that affect original functional design specifications their associated labor and travel expenses e Unlimited access to Zymark s Technical Support Center during the warranty period which provides troubleshooting repair instruction service dispatching replacement part information and shipment e One pre scheduled on site Preventive Maintenance and Diagnostic Servicing PM performed by a Zymark Field Service Engineer A PM visit includes the replacement of Limited Life parts exposed to fluidics such as syringes valves seals and fittings The Zymark Field Service Engineer will perform a comprehensive analysis and testing to confirm the equipment is left operating to factory specifications e Scheduling of the PM within the warranty period is flexible and determined by the customer after notification by the Zymark Field Service Engineer The servicing is typically scheduled six months after the equipment installation but equipment usage rate and availability may cause the date to vary e Acompleted Zymark Field Service Report provides thorough documentation of all maintenance and service work performed by the Zymark Field Service Engineer during the on site visit Exception Documentation is not provided when Zymark provides service via telephone fax or modem e Limited Life parts are covered for thirty 30 days after installation and are r
88. ht characters plus the three letter extension e Although the Reagent Screen data is now saved as a file it is not yet active for use Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Software 23 Recalling a Saved Set File and Making it the Active File The reagent setup file must first be recalled before a method can be created or run 1 From the Development screen click on Reagent Setup 2 Default entries appear in all the fields The test1 set screen does not appear because you did not click on the OK Save as current button to make it active for use NOTE H To make a screen active for use click on the OK Save as Current button Once you click on the OK Save as Current button this instructs the software to use these setup parameters when creating and running a procedure 3 Inthe Reagent Setup screen click on File and Open Choose the test1 set file and click on OK The data in test1 set will reappear in the fields 8 Click on the OK Save As Current button This will bring back the Main menu 9 Click on Reagent Setup The data from test1 set is now your active file for use until the you select the defaults or another set file w Reagent setup for all modules olx File ModuleNumber Reagent Reagent Name Abbreviation Sip
89. illuminate If an error light is on refer to Troubleshooting p 78 Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Hardware Check After initialization is complete check the hardware to confirm that RapidTrace workstation is ready to run procedures Item Power Light Run Light Error Light Module ID Syringe Confirm that Green light is illuminated Run light is OFF Error light is OFF Each module has a unique module identification number Syringe is at its HOME position The syringe plunger is clean and that no liquid is on top of the syringe plunger Installing RapidTrace Hardware 5 Solution Confirm that RapidTrace is switched ON A run is in progress Wait until the run is complete or stop the run Go to the ERROR RECOVERY screen Select STATUS Correct the problem Correct so that each module has a unique number assigned to it Go to the ERROR RECOVERY screen and move the syringe to the HOME position The Home position is the point at which the plunger is at the bottom of the syringe 0 mL with a small gap If the syringe is not clean run the recommended cleanup procedure detailed in Cleaning the RapidTrace Modules Post run Cleanup Procedure p 53 P N 55078 Rev 5 RapidTrace SPE Workstation Installation M
90. ing the return of equipment to Zymark equipment must be returned to Hopkinton MA USA or a facility designated by Zymark The customer shall prepay charges for equipment returned to Zymark with Zymark paying for equivalent return shipment to the customer A Returned Material Authorization RMA must be obtained for any equipment being returned to Zymark Contact the Zymark Technical Support Center by telephone at 508 435 9761 or via the Internet at techsupport zymark com or by fax at 508 435 0950 before returning any equipment to Zymark Additionally customers are required to complete a Zymark Chemical Questionnaire prior to the issuance of an RMA All equipment returned to Zymark must first be decontaminated to meet Zymark and United States Department of Transportation procedures and standards These procedures provide for the safety of Zymark receiving and repair personnel Hazardous Limitation Statement At no time will Zymark personnel perform service on unsafe equipment perform service in unsafe environments or decontaminate equipment to make it safe Prior to the performance of any service work Zymark personnel will evaluate the condition of the equipment and the environment in which the equipment is located After evaluation and discussion with the customer Zymark commits to the following e When the environment and equipment are determined to be free of chemical radioactive and bio hazardous materials by Zymark personnel servi
91. int Selected Reports One report may be printed at a time when a printer is connected to the RapidTrace Controller To print click on the box of the report you wish to print then click the Print Selected Reports button The report will print to the screen s default printer Click on Exit to return to the Main menu Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running Methods and Monitoring Workstation Operations 47 Magnetically Encoded Racks Magnetically Encoded Racks enable the user to designate a specific procedure to a rack Many laboratories find this useful in production mode When using magnetically encoded racks the procedure does not have to be assigned to samples in the Setup Rack screen because the module will read the magnets and automatically know which procedure to run The module will run the number of samples corresponding to the number of sample test tubes placed in the rack Ee Only one procedure may be assigned per rack Follow these steps to magnetically encode a rack 1 Write the procedure you wish to magnetically encode using RapidTrace development and Create Procedures as you would any procedure All magnetically encoded procedures must contain at least one Collect Fraction step 2 Encode the rack by placing magnets in the rack in a sequence representing a code
92. ion Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Software Overview 9 RapidTrace Software Overview Software Characteristics The RapidTrace SPE software has the following distinct characteristics e Employs Windows based software with user friendly interfaces e Allows for all samples to be run under the same procedure or individual samples to be run under different procedures e Simultaneously processes up to ten samples in a ten module workstation e Collects one to ten fractions per sample e Controls the flow rate for each step in the procedure e Segregates up to three types of waste from the column and one type from the cannula e Links procedures to a magnetic code for ease of operation RapidTrace Icons There are four RapidTrace application icons Development Production Magnetically Encoded Rack and Load RapidTrace Firmware Some users may not have access to all the icons due to security levels at your company Description y RapidTrace Methods Allows access to Development Icon Reagent Setup Create Procedures Comm port selection Flow rate Setup Racks Run Monitor Reports Error Recovery On line Help a RapidTrace Production Allows access to Icon Setup Racks RapidT race Run Monitor Production Reports Error Recovery On line Help Magnetically Encoded Allows you to assign your procedure to a a Racks coded rack R
93. is cable into the bottom receptacle of the next adjoining module Connect the Power Cord Connect the power cords to the back of the modules and to the power source Reagent Lines You may connect the new module using individual reagent lines provided in your startup kit or you may add the new module to your manifolded workstation Connect Individual Reagent Lines 1 Match the corresponding labeled reagent line on the module with the line from the Individual Reagent Lines kit and tightly twist the two connectors together Repeat for all eight lines Refer to Installing RapidTrace Hardware Reagent Lines option 2 p 6 for additional information on Individual Reagent lines Place the reagent lines in the reagent bottles and place them in an empty container to keep them clean P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 70 Adding or Moving RapidTrace Modules Add a Manifold Purge Reagent Lines Fluid must be removed from the reagent lines 1 Remove all the lines from the reagent bottles and place them into a clean beaker or container to keep them clean 2 Write a procedure to purge the reagent lines taking their miscibility into account An example of a procedure for eight reagents follows Create procedure using reagent names for all modules File Edit Reagents Setup
94. lation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 52 Daily Operations Prime the Reagent Lines Prime the reagent lines before running samples on a daily basis Also prime reagent lines whenever new reagents are added To prime reagent lines write a procedure using the Purge and Clean Cannula step for each reagent line that needs to be primed The recommended volume for each Purge and Clean Cannula step is 6 mL with a flow rate of 30 mL min 0 5 mL sec The procedure shown below is an example that will prime four reagent lines with 6 mL of each of four reagents Zymark recommends that this procedure be run with two samples which would prime each of the reagent lines with 12 mL a volume that is sufficient for either individual or manifolded reagent lines The procedure shown below will prime reagent lines 1 4 Procedure Name C SPE PURGE4 SPE Liquid Source Output Vol ml min Sense 1 Purge Cannula H20 Cannula 6 30 No 2 Purge Cannula rae teamaete se No 3 Purge Cannula MeOH Cannula No 4 Purge Cannula H20 Cannula No 5 Purge Cannula Buff Cannula No 6 Purge Cannula H20 Cannula 6 30 No NOTE L To prevent salting out of buffers confirm that organic solvents are always preceded by and followed by water not buffers Always use water as the first and last reagent to purge the lines
95. les Be P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 26 RapidTrace Software Creating Procedures Features of the Create Procedures Screen To access the Create Procedures screen from the Main menu click on Create Procedures This screen is used to write edit and save SPE procedures Create procedure using reagent names for all modules _ ol x File Edit Reagents Setup Variables Password Procedure Name squid Created 1 13 98 2 01 23 PM Step Source Output Vol ml min Sense a a ane a aS Last Modified No a No No Run Time No No No No No No Procedure Description Con rnnenw nas Under the title bar there are five pull down menus File Edit Reagents Setup Variables and Password File Opens a New file Opens an existing file Deletes a file Saves As another file name and Prints a file Edit Inserts a line in a procedure or Deletes a line from a procedure Reagents Allows you to use previously defined Reagents while writing a procedure If you have a Reagent setup active for an Individual module you can pull down the Reagents menu and select the module number to use the Reagent setup as you write your procedure The software defaults to the
96. llation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 50 Running Methods and Monitoring Workstation Operations Running a Procedure with Magnetic Racks Place SPE columns in the turret and test tubes in the rack Be sure to place the number of test tubes in the rack equal to the number of samples you want to extract Place the rack into the RapidTrace workstation NOTE L Set up the Controller in the Run Monitor screen using either the Production software or the Development software Push the Start Stop button located on the front of the module to start the magnetically encoded procedure ce When the Start Stop button is pushed the magnets are read only A rack with magnets in place may be used in non magnetic mode simply by using the Setup Racks screen and the Run or Run All button on the Run Monitor screen to start the run Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Daily Operations 51 Daily Operations Daily Operation Checklist Preparing the Many of the steps below use specific RapidTrace screens You can access all screens from the Zymark RapidTrace SPE Workstation screen activated through the Development icon Workstation When preparing the workstation check the reagents to determin
97. ly Periodically check waste lines for damage Confirm that flow is not inhibited and that the waste container is large enough to hold drainage Always exercise extreme caution when handling fluids in the laboratory Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installing RapidTrace Software 7 Installing RapidTrace Software Installing the Software STEP 1 Loading the Software and Firmware STEP 2 Installing RapidTrace Software Files STEP 3 Installing RapidTrace Firmware Files STEP 4 Create the Four RapidTrace Icons The RapidTraceSPE Workstation software can be installed on a computer with Windows NT 4 0 service pack 5 or greater U S version Operating System with 16MB of RAM All Zymark RapidTrace Controllers have the RapidTrace software pre installed at the factory You will have a RapidTrace Program group with four icons RapidTrace Magnetically Encoded Rack Zymark Load RapidT race Firmware RapidT race Development In your RapidTrace Order Kit you have two RapidTrace software disks You also have a firmware disk To install the disks follow the applicable instructions for installing the files and creating the icons In File Manager create a directory called spe Place the first disk RapidTrace Software into your disk drive and copy
98. n A keyword search can be run by clicking on Search in the upper left corner of the Help screen RapidTrace File Edit Bookmark Options Help Conen Seon ooo Bin This is the Help Topics Search screen Help Topics RapidTrace 21x Index Find 1 Type the first few letters of the word you re looking for oo 2 Click the index entry you want and then click Display 12 Port Valve Add reagentto mixing vessel Adding a Reagentto a Sample and Mixing Adding a Reagentto the Mixing Vessel Application Tips Biohazard Waste Cannula Cleaning Up After Running SPE Procedures Clear Collect fraction Collecting a Fraction Collection tube Column Column air push volume Display Brint Cancel e Click on a hotspot area in the screen for additional information on that feature The hotspot areas are denoted by a green shade and the mouse arrow turns to a hand as you pass the arrow over the hotspot e Click on Contents and choose the hotspots in sections of interest P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 12 RapidTrace Parts and Functions RapidTrace Parts and Functions Front Features Figure 1 RapidTrace Front View WARNING AAs
99. n the screen the test1 set file is NOT active for all modules because the default entries have been assigned to Modules 0 through 5 Click on Module Number click on the circle for Module 0 and click OK The default entries are active for Module 0 Repeat the step above for Modules 1 through 5 All other modules will have the test1 set Reagent screen data assigned Double click on the Close button x in the upper right corner to close the Reagent Setup screen to return to the Main menu Or Close the screen using the close command from the pull down menu in the upper left corner of the screen NOTE In order to save the changes you made press the OK Save as Current button 17 18 e From the Main screen click on Reagent Setup The test1 set data will appear in the fields and will seem to be assigned to all modules however because of the actions taken in Steps 1 6 above the default entries actually have been assigned to modules 0 through 5 To make test1 set data active for all modules click on the OK Save As Current button The default entries are now removed for modules 0 through 5 and the data from test1 set will now appear for each module Since the default in the Module Number screen is All Modules any updates made to the Reagent Setup screen that is followed by clicking on the OK Save as Current button will affect all modules unless a specific module is chosen first O Module 9 All Modu
100. ning the Variables p 21 Vent Water Methanol Acetone Isopropanl Toluene Hexane Tetrahydrofuran Ethanol Mixing Vessel 22 Click once on Water Its abbreviation appears in the cell for the Source W Create procedure using reagent names for all modules File Edit Reagents Setup Variables Password ay es Siep Source Output 1 23 Click once in the fourth box on the first line directly under the word Vol This column denotes the amount of Source in mL that will be added to the Sample Type 5 in the box This will cause 5 mL of water to be added to the sample 24 Click once in the fifth box of the first top line directly under mL min The number input will determine the flow rate of the water being added to the sample Type 30 in this box NOTE n The rate at which water is pulled out of the reagent bottle is defined by the sip speeds in the reagent setup screen 25 Click on the word No in the first line and the Column labeled Liquid Sense No will become Yes This will cause the liquid sensor to detect if there is liquid in the syringe If there is no liquid the module will display an error light Failure to pull liquid could occur if the reagent bottle is empty or a blockage exists in the fluid path Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation
101. nt Lines Purge Reagent Lines Fluid must be removed from the reagent lines To remove fluid from the reagent lines do the following steps 1 Remove all the lines from the reagent bottles and place them in a clean empty container 2 Write a procedure to purge the reagent lines taking the miscibility of the reagents into account For eight reagents an example of a procedure follows te Create procedure using reagent names for all modules Oy x File Edit Reagents Setup Variables Password Source Output Vol mimin Step 1 Purge Cannula Reag1 Cannula 6 5 2 Purge Cannula Reag2 Cannula E 3 Purge Cannula Reag3 Cannula i 4 Purge Cannula Reag4 Cannula i 6 Purge Cannula Reag6 Cannula 8 Purge Cannula Reag8 Cannula Liquid Created 478798 3 53 30 PM No Hy Last Modified 4 8 98 3 55 35 PM Run Time 101 9 No Procedure Description No This procedure purges all eight reagent lines AnH A nnn in en en en enj cn en 66 Inthe Setup Racks screen assign the procedure to the first two samples of each module being moved 67 Run the procedure for all modules to be moved A test tube will need to be in position in sample position 1 on the rack When the modules complete the procedure check the lines for fluid Disconnect Plumbing When the fluid has been removed from the lines the plumbing can be disconnected WARNING A If gas
102. ocedure similar to the one shown below Procedure Name PURGE8 SPE urge Canmnula var e po uige Cannula Reag2 Cannula 6 30 urge Cannula Reag3 Cannula 6 30 uige Cannula Reat Cannula 6 30 urge Cannula Reag5 Cannula 6 30 uige Cannula Reas Cannula 6 30 Purge Cannula Reag Cannula 6 30 Purge Cannula Reag Cannula 6 30 Purge Cannula Reagt Cannula 6 30 Con none wn Reagent lines should be pulled out of the reagent bottles and placed into a beaker of de ionized water Purge all reagent lines Remove the lines from the water and run the purge procedure once again to dry the lines Repeat this procedure placing the reagent lines in a beaker of methanol priming the lines and once again running the procedure with the lines out of the methanol beaker to thoroughly remove the methanol from the fluid path By removing all traces of water the chance of bacterial growth and or rust is eliminated The methanol will serve to disinfect the fluid path and any last traces will quickly evaporate P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 92 Reference Data Extended Inactivity Procedure Use the following procedure to safely and effectively prepare the RapidTrace workstation for long term shutdown Run the procedure on all modules 1 RunCLEAN SPE one
103. ot available go to the Windows Explorer screen and double click on the file named load bat in the subdirectory named rload A DOS screen will appear with the message Loading RapidTrace Firmware Please Wait When the cursor stops spinning the download is complete During the download the PWR RUN and ERR LED s on Module 0 will be ON The module will re initialize once the download is complete Repeat steps 1 3 for each additional module P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 78 Service Troubleshooting Potential problems are presented with possible solutions in this section If further problems occur or if the problem is not corrected call the Zymark Technical Support Center for assistance Refer to Technical Support in the Preface for contact information See Parts and Functions p 12 for more information Problem Solution Sample Fraction 1 Check for missing test tube or wrong test tube type Tube not detected 2 Ensure that all fraction and sample test tubes are in place 3 Use glass test tubes if possible 4 Clean sensor with a cotton swab dipped in isopropyl alcohol 5 Go to Error Recovery Screen Fill the rack with test tubes Send the Shuttle Waste command Send the Rack Command The Reply Message should read Rack XXXX O3FF O3FF If not adjust the senso
104. r position by loosening the thumbscrew located at the bottom of the sensor 12 Port Valve 1 Turn the brass disc on the back of the 12 Port Valve through 2 full positioning error rotations Access this valve through the slit in the side of the module or by removing the upper section cover 2 Resume the run Blocked syringe discharge l Go to the Error Recovery screen Draw up 6 mL of water into syringe 2 Disconnect tubing from syringe base Set flow rate to 42 mL min 0 7 mL sec and empty syringe into a beaker This should unclog the syringe Rack missing or not 1 Confirm that the rack is properly engaged on the two pins of the shuttle t huttle comered On akai 2 Go to the Error Recovery Screen Send the Shuttle Waste command The Error light should not go on Send the Status Command The Reply Message should show Stopped not Error Column bed not aligned 1 Confirm that the SPE columns are properly installed and that the turret not sensed or too small is aligned with the column plunger 2 Go to Error Recovery and send the Warm Start Command Once initialization is complete restart the run Refer to Column Bed General p 87 for more information Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Problem Magnetic Rack Error Messages appear or wrong procedur
105. r space requirements Height 10 in 25 4 cm Width 11 in 27 9 cm Depth 9 in 22 9 cm Additional Space Requirements Space will need to be provided to access the power cord power switch RS 232 communications cable ports and tubing at the back of the workstation Space will need to be provided for reagents at the bench level The RapidTrace module can be individually plumbed and use up to 8 reagents or can be manifolded together so that the entire workstation uses up to 8 reagents Work Area Flat stable level surface Weight 27 lbs 12 3 kg per module Compressed Gas Supply optional feature Clean dry regulated air or nitrogen etc Fitting for Gas Supply For connection of gas supply to fit 1 8 ID gas tubing Compressed Gas Supply Inlet Pressure 10 psi 0 7 bars minimum 40 psi 2 8 bars maximum WARNING A A To avoid personal injury or damage to the instrument DO NOT exceed 40 psi 2 8 bars maximum inlet pressure Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Unpacking and Site Preparation 3 Cables A standard 5 ft 1 5 m RS 232 communications cable with male and female 9 pin connector ends is supplied with the RapidTrace A second standard RS 232 communications cable is supplied for interconnection to another module Phone Lines for Data Transf
106. r the procedure or the code number you are running Magnet Table O 990 9090 STS TSS QOO OBWBWOWOO Oe BOO O 9999999000000 BORO OOO p o 0000 a O OOO COS OOOO Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running Methods and Monitoring Workstation Operations 49 Assign the Procedure to the Code 1 Double click on the Magnetically Encoded Rack icon 2 Click on the desired procedure from the box on the left side of the screen Assign Magnetic Rack codes to Procedures V1 20 _ ol x Procedures on file Procedures assigned to rack codes Selected Procedure Description pe Delete selected item OK Save Help 58 Click on the code number to be assigned from the box on the right side of the screen 59 Click on the arrow button between the two boxes The procedure will appear next to the code number Assign Magnetic Rack codes to Procedures Y1 20 O x Procedures on file Procedures assigned to rack codes Code 1 Iml spe Code 2 Code 3 Code 4 Code 5 Code 6 Cnde 7 60 Click on the OK Save button The procedure will be assigned to the code P N 55078 Rev 5 RapidTrace SPE Workstation Insta
107. re 12 Connecting the Gas Line Waste Lines The four waste lines extend from a cutout at the base of the rear of each module Se ff Cutout for waste lines G4 Figure 13 Cutout for Reagent and Cannula Waste Lines The 10 foot waste lines remained with the waste receptacles when the modules were moved 1 Move the 10 foot lines to the modules ensuring that each line is attached to its corresponding numbered line on the module 2 Place the line in the appropriate waste receptacle 3 Check the Reagent Setup screen for your designation of Wastes 1 2 and 3 Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Adding or Moving RapidTrace Modules 69 Adding a Module to the Workstation NOTE L Only qualified personnel should install RapidTrace modules Add the module to the end of the workstation furthest from where the reagent lines enter into the reagent bottles Set the address of the module appropriately Connect the Communications Cable s 1 Locate the RS232 communications cable that goes to the Controller Disconnect this cable from the bottom receptacle of the module adjacent to the new module and place it into the bottom receptacle of the new module Attach the new RS232 cable from the startup kit to the top 9 pin connector of the new first unit Plug the other end of th
108. recetenceens 59 Magnetically encoded racks 0 ce eeceseeeeeeeeeeeeeeees 47 Manifold setup ccccescsseesseereeeeeeerceseceeeceeenseenees 61 Manifold adding cecceseesceesseeseeeseeeeeeeeceteeeseenees 70 Material Safety Data Sheet cceeceeseeeeeeeereeeeees 6 Material Safety Data Sheet MSDS 4 16 51 Mixing capability cceeseeeeeeeceseceseeeeeeteeseeenees 86 P Parts and functions back view ccceceecssecessceesseceeeeecsseceseeeesseesteseeaeeees 14 cannula vases coc cecescecstscesecesseceaseevoned essei sissorni vissri teise 90 column PIUNET eee ceeeecesecteeeeeeeeeeeeceeeeeeaeeeees 90 standard rack 2s 0 22 sdsdeicsseescecaeci csmnaasioeneicees 1 90 Phone lime eecceceeccecssecessceescecsseceeseecsseseeaeecseeeeseeees 3 Plumbing dia prai c5 ns Rooce neti tirep n ai abies e Eas 16 individual reagent lines cceeeeceee 61 66 69 PO WOE 25 503 scctee tft oethas Geet aR Pa EE ER EE EREE 86 FUSES neneeese anenee ne ieaie 3 line voltage isorine eia 3 requirements line MEQGUETICY ic csesececsbaceccessesceanvonsssacotasneseseies 3 WALES irern inre dines ouch E E E E E EENES 3 Product information cccccccceesscceesssceceessseeeeeseees ill PUPSC CeSt sc niisiis esnaiesaciees heeuts exits cetnies 73 P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE
109. reen click on Create Procedures Click on the word File at the top left of the screen and click on Open Click on the file test 2 spe then press OK The default extension for RapidTrace gt procedures is spe Click on the Step box for the fourth line The fourth line is the line that contains the Rinse step ts Create procedure using reagent names for all modules Of x File Edit Reagents Setup Variables Password Procedure Name Liquid Sense __Step___ Source Output Vol Add to Sample H20 Cannulals s0 No Condition MeOH Ow f5 fe Noo oad Sample Aquw 5 8 12 No Rinse Ace Oww 6 B Collect MeOH Fract 6 1 2 No PurgeCannula W20_ Cannula 6 30 No No Created 3 26 98 3 39 48 PM Last Modified 3 26 98 3 39 53 PM 3 39 53 PH Run Time ss Procedure Description 1 2 Click on the word Edit directly below the Title bar and click on Insert A new line has been added above the highlighted step With the first box in the new line still highlighted click on Edit then click on Delete The new line is now deleted You can also edit an SPE procedure by changing its volume flow rates reagents or other properties After edits have been made press OK Save to save the edits to the file The edited version of the file can also be saved under another name by using the File Save As commands from the pull down menu at the top left of the screen
110. rkstation Version 1 20 Comm Port Flow Rate Units 2 The Comm Port screen will pop up Port number i Ea 3 Delete the comm port number and type in a new number Then click on OK 4 Ifthe port specified is not available on your computer the error message Port Unavailable will be displayed Click on OK and change the Comm Port to an available port number Click on OK again to return to the Main menu Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Software 19 Flow Rate Units The second feature in the Development Main menu is the ability to set Flow Rate Units You may change the units to mL sec or mL min The default flow rate is mL sec 1 Click on the words Flow Rate Units located below the title bar The Flow Rate screen will open Comm Por Flow Rate Units 2 Click on the circle corresponding to mL min or mL sec and click on OK The software will now show all flow rates in that selection NOTE L For RapidTrace Development use continue to the next section Reagent Setup For RapidTrace Production go to Setup Procedure Sequence Screen p 38 P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com
111. rocedure is calculated The software will calculate the run time after the procedure is saved P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 28 RapidTrace Software Features of the Create Procedures Screen Continued Procedure Description Specific information about the procedure may be typed into this field The software will display this description in the Setup Racks screen to help in procedure identification Information about the procedure such as reagent set used or the SPE column type may be entered here The Procedure description will be shown when assigning procedure in the Setup Racks screen Procedure Description ee The description must be a series of words and spaces Continuous letters will not print to hard copy Writing an SPE Procedure Name the Procedure 1 Click on the Create Procedures button from the Development Main menu w Create procedure using reagent names for all modules _ ol x File Edit Reagents Setup Variables Password Procedure Name FE Grae 1 14 98 4 35 01 PM Step Source Output Vol ml min Sense 1 No Last Modified 2 No 3 Run Time 3 No al No Procedure Description 5 No 6 No 7 No 8 No 9 No 10 No 11
112. ror conditions Macros group the individual operations together to perform a task See Using Macro Routines p 62 RapidTrace Error Recovery me Syringe Twelve Port Valve Shuttle Sample Shuttle Collect Shuttle Waste Continue Warm Start Reply Message _Help_ NOTES H e There is no error checking when operating in the Error Recovery screen e Do not attempt to exit the Error Recovery screen when the computer is executing a command or doing an operation when the mouse cursor is an hour glass or the computer will hang The computer will need to be rebooted i e switched off then switched on P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 56 Error Recovery Functions The box in the upper left corner lists the individual modular functions Syringe Twelve Port Yalvye Shuttle Sample Shuttle Collect Shuttle Waste Shuttle Wash Shuttle Out Column Plunger Continue Warm Start Module Number The Module box in the middle of the screen shows the module number to be operated Only one module can be operated at a time unless a 10 is entered in this field Entering a 10 in this box causes all the modules to run Module fo E Macro Routine Buttons The four buttons in the lower right corner of the screen are for clearing saving rec
113. rporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Service 85 Zymark Product Repair Depot The Zymark Product Repair Depot offers product repair services upgrades refurbishment and installation at reasonable rates with quick turnaround times for customer owned equipment and accessories Shipping to and from Zymark is the responsibility of the customer including specifying the carrier and the choice of service NOTE L To ensure a safe environment for all technicians it is mandatory for each returned product to include our chemical questionnaire stating contact chemicals chemicals used during application and cleaning steps taken prior to shipment Upon receipt the product will be evaluated for necessary repairs and you will be contacted with an estimate At that point you decised whether servicing will commence or be discontinued If service is declined a minimum evaluation charge may apply Upon completion of the repair a purchase order or appropriate means of payment is required before return shipment P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 86 Reference Data Reference Data Specifications Power Weight per module Size lx wxh p
114. rtisantg com 14 RapidTrace Parts and Functions Back Panel Features Figure 2 RapidTrace Back View DANGER OA Hazardous voltage still exists within the workstation when the AC power is switched OFF Always unplug the power cord if changing fuses or servicing the equipment Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Parts and Functions 15 Table 1 Front Parts and Functions Item 1 Part Hole for reagent and gas lines Exhaust Fan RS232 Communications Cable Connector Output Power Switch Power Entry RS232 Communications Cable Connector Input Waste Lines Function e 8 reagent lines e Gas line Ventilates the internal electronic components When multiple modules are used the RS232 Comm output is used to connect the communications cable to the next module Switches the module AC power on or off Connects the unit to power source via an AC power cord and houses the fuses Connects module to the Controller if it is the first module or connects module to the output of the previous module 3 column waste lines and 1 cannula waste line P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE
115. seses iii 84 A 0 sne n n E EA 3 86 Trainin seee E 84 Troubleshooting tables ccccsceesceeseeteeeteeeteeees 78 U Unpacking 0 eeccecceesceesecscecseeeseeeeeeaeecaeeeaeeeneeeeeeereens 1 yV Voltage specifications s sssssssesesessesreseressreersseeees 87 wW Watantin eein E ERE iii Waste containers cccccccceceseesesssceseeecessesseeseesseeseeees 3 Waste lines connecting essesseseeesssreeesessersersreee 72 Waste lesi eea a S 74 Weightecnnnn mnn R RERA 2 Work AL ceceseesssssccsccecsessssscesssessessesseeseseseeseeees 2 Z Zymark Corporation IN EULOPe eeeeceesceeceesceseeesceeeeeeeeeeeceeenseenseenseenaes iv intemmet address sce csisesi cca reer r aer Errr E iii Technical Support Center ssssseeseeseeeeeseeees eese iii Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Zymark Zymark Corporation Zymark Center Hopkinton Massachusetts 01748 1668 508 435 9500 Fax 508 435 3439 Internet techsupport zymark com Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com A rtisan Artisan Technology Group is your source for quality TecmoogyGroup new and certified used pr
116. setup for all modules Setup Variables Sets the sip speed for the mixer the column air push volume and the column air speed multiplier Password Allows you to set a password for your procedures and Reagent setups Setup Variables Sip Speed The Sip Speed is the flow rate at which the syringe will pull the reagent from the mixing vessel or sample On the Create Procedure screen click on Setup Variables Set the Sip Speed and click on OK Column Air Push Volume Air is used to push reagents or sample through the fluid path to the column to clear the Reagent line The air push volume is a specified number of mL ft depending on the size of the column barrel and the depth of the column bed The volume range is from 1 mL to 6 mL with a default value of 2 mL Column Air Push Speed Multiplier Use this factor to increase the airflow rate through the column after a reagent or sample step The slow liquid flow rates required to push reagents or samples through the SPE column are not usually necessary for the air push after a reagent or sample step This parameter can be used to speed up the run time for the procedure without decreasing recovery Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Software 27 Create procedure using reagent names for all modules File Edit Reagents
117. step occurs after Step 8 in procedure the collect step and should use the elution solvent typically a strong solvent that will thoroughly strip the cannula of any remaining analyte eliminating carryover between samples Third Purge Cannula The third Purge and Clean Cannula step occurs as the Step 9 in procedure last step in the procedure and always uses water Never use a buffer as a last step because that would leave buffer salts in the fluid path P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 38 RapidTrace Software Setup Procedure Sequence Screen Main Features The Setup Procedure Sequence screen is to assign procedures to samples in a rack for each module The same procedure can be assigned to all samples in a rack or a different procedure can be assigned to each sample in the rack You can access the screen by clicking on the Setup Racks button from either the RapidTrace Development or Production icons f Setup Procedure Sequence OY Xx Procedures on file Procedures assigned to Module 0 waste spe Delete selected item IK Module 0 X Module 5 Selected Procedure IX Module 1 X Module 6 Description IX Module 2 x Module 7 X Module 3 x Module 8 IX Module 4 x Module 9 Select All Clear OK Save Help e The box in the top left corn
118. tg com 76 Service Unblocking a Syringe Discharge Tube The syringe discharge tube is the horizontal stainless steel tube that connects the base of the syringe to the 12 Port Valve If this tube becomes blocked a blocked syringe discharge error message will be displayed WARNING A Always use extreme care when handling fluids in the laboratory To unblock the tube follow the procedure below 1 e 82 83 84 85 86 87 88 Go to the Error Recovery screen in the Rapid Trace Software Send the 12 Port Valve to the reagent bottle containing water For most applications water should be assigned as Reagent 1 Send the Syringe Position to 6 mL This will fill the syringe with 6 mL of water Disconnect the small length of Teflon tubing running from the syringe discharge tube to the 12 Port Valve Place a small beaker or other appropriate vessel under the tube to catch the liquid Set the Syringe Speed to 0 7 mL sec 42 mL min This is the fastest syringe speed Set the Syringe Position to 0 mL This will rapidly empty the syringe removing the blockage to the beaker Reconnect the tubing from the syringe to the 12 Port Valve Send the Warm Start command to re initialize the unit and confirm that the syringe discharge is unblocked If the syringe discharge is still blocked repeat the above steps but change the syringe speed in step 4 to 0 1 mL sec The slower motor speed will produce more torque
119. that the module is performing or any errors that have occurred w Run RapidTrace OL x Beep Module 0 Module 1 Module 2 Module 3 Module 4 Run Stop Run Stop Two Modules Running TEST2 SPE Procedure Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running Methods and Monitoring Workstation Operations 45 View and Print Run Reports Main Features of the RapidTrace Reports Screen The RapidTrace Reports screen displays the last three reports from each module Ee The reports are listed in no particular order so pay close attention to the date and time to determine which report you wish to see The main functions of this screen are to view and print Run reports and to access the sample counter for each module with the View Usage Log button To get to this screen simply click on Reports from the Main menu w RapidTrace Reports Mikl x Module Start Time ss Start Time Start Time 01 12 16 97 11 04 25 12716797 11 05 26 1 21 98 9 37 24 AM 11 20 97 4 45 49 PM 11 20 97 4 50 40 PM 6 5 97 2 55 56 AM 4 4 95 2 06 46 PM 6 5 97 2 57 37 AM 675797 2 57 37 AM 6 5 97 3 49 36 AM 675797 3 00 07 AM Print Selected Reports Exit View Usage Log _Help RapidTrace Reports Screen P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark
120. ting Fraction s Refers to the material packed into the SPE column The first step s in most SPE procedures preparing the column bed to bind the analyte Referred to as solvation or activation of the column bed A step done post extraction to bind a group to the analyte to enhance detection The step s used to remove analyte s of interest from the SPE column May be done in single or multiple steps When analyte s are eluted from the SPE column they are eluted into volumes called fractions Single or multiple fractions may be collected This interaction occurs between an analyte carrying a charge positive or negative and a sorbent with the opposite charge The step when the sample is put onto the column The initial sample which is most commonly plasma urine or serum May also be tissue extracts or other bodily fluids such as bile meconium etc The chemistry used to achieve the separation in multiple modes or more than one mode The two or more modes are mixed together in the column Means used to achieve the separation for example in a reverse phase separation the mode of the separation is reverse phase P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Glossary Continued Normal Phase Packing Material Particle Size
121. tions 2 To program a group of modules rather than the entire workstation click on the X in the boxes for Modules 0 1 2 3 and 4 The X will disappear so these modules will not be selected when you assign procedures to the samples 51 Assign the 3ml1 spe procedure to all the samples by clicking on the 3m1 spe procedure highlighting Samples 1 through 10 and clicking on the arrow button in between the boxes The 3m1 spe procedure is now assigned to all the samples for Modules 5 6 7 8 and 9 Modules 0 1 2 3 and 4 will still have their previous procedures assigned to them Module 0 Module 5 Module 1 X Module 6 Module 2 X Module 7 Module 3 x Module 8 Module 4 1 Module 9 52 Clicking on the OK Save button in the lower right corner of the screen returns you to the Main menu 53 Click on the Run Monitor button to return to the Run Monitor screen Notice that the Run All button has now disappeared because different procedures have been assigned to individual modules 54 Click on the Exit button in the lower right portion of the screen to close the screen and return to the Main menu gt Once a module is running the Controller has downloaded the necessary information into the module and you are free to use the Controller for other purposes However for module monitoring purposes you may remain in or return to the Run Monitor screen at any time Each module s box will display information on the step
122. tle is out first send the shuttle to Waste or Wash before sending it to a sample position Shuttle Wash Moves shuttle to canula wash position cannula above cannula waste station Shuttle Waste Moves shuttle to designated waste line Status While the RapidTrace module is running an SPE procedure the module s current status such as Stopped will be displayed here If there is an error condition and you select Status and then choose the Send button an error message displays in the Reply Message field Be sure that you have selected the correct module in the Module field Syringe Position Positions the syringe plunger in the barrel For example a value of 0 places the syringe directly at the bottom of the column a value of 1 mL places the syringe about 0 24 above the bottom of the barrel You may specify a value between 0 and 6 mL inclusive This command will function with the shuttle in any position gt Whenever a procedure stops running Syringe Position should be reset to 0 zero before resuming the procedure run Syringe Speed Sets the Speed mL s that you want the syringe to use when delivering liquids The range for syringe speed is 0 006 to 0 7 mL s During error recovery the syringe speed may need to be adjusted The syringe speed remains at speed of the previous procedure until it is changed Turret Moves the turret to the designated column number The rack must be in the waste or
123. un a purge test and a waste test Begin by turning on the modules Observe them as they initialize Initialization is a self test run by a module every time it is switched on It assures that all components of the RapidTrace are in correct position to start running samples and it confirms that the RapidTrace itself is properly functioning If an error is encountered during initialization the error light will illuminate Refer to Installing RapidTrace Hardware Initialization p 4 Purge Test Run a purge test for all modules in your workstation to prime the lines with reagent and check for leaks For a Manifolded Workstation 1 Write a procedure to purge the reagent lines taking the miscibility of the reagents into account For eight reagents an example of a procedure is shown below Create procedure using reagent names for all modules Ol x File Edit Reagents Setup Variables Password Procedure Name PURGE8 SPE iquid Grosted Step Source Output Vol ml min ams 1 Purge Cannula Reag1 Cannula 6 30 No Last Modified 2 Purge Cannula Cannula 6 Run Time 3 Purge Cannula Reag3 Cannula 6 30 No 4 Purge Cannula Reag4 Cannula 6 30 No Procedure Description 5 Purge Cannula Reag5 Cannula 6 30 No This procedure purges all eight reagent 6 Purge Cannula Reag6 Cannula 6 30 No lines 7 Purge Cannula Reag Cannula 6 30 No 8 Purge Cannula Reag 8
124. using reagent names for all modules _ ol x File Edit Reagents Setup Variables Password Procedure Name CLEAN SPE ae Created 11 13 95 8 21 56 AM Step Source Output Vol ml min Sense 1 Purge Cannula H20 Cannula 3 24 No East Modified 991 20 98 3 28 49 PM 2 Add to Mixer Sample Mixer 2 5 j15 No Run Time 7719 3 Purge Cannula Mixer Cannula 2 6 30 No 4 Add to Mixer Hed Mixer 4 5 30 No Petia eecunnen Purge Cannula Mixer Cannula 5 30 No This is the template to use for the 6 Add to Mixer H20 Mixer 4 5 30 No cleaning procedure 7 Purge Cannula Mixer Cannula 5 30 No 8 Add to Mixer H20 Mixer 45 30 No 9 Purge Cannula Mixer Cannula 5 30 No 10 Load Sample Aqu 25 f5 No 11 Rinse H20 Aqu 3 18 No 12 Rinse H20 Aqu 3 18 No 13 Rinse H20 Aqu 3 18 No 14 Load Sample Org 25 f5 No 15 Rinse H20 Org 3 18 No 16 Rinse H20 Org 3 18 No 17 Rinse H20 Org 3 18 No 18 Purge Cannula H20 Cannula 5 30 No 19 No 20 No OK Save Help Procedure Screen for Cleaning P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 54 Daily Operations Post run Cleanup Procedure Continued Reagent setup for all modules io
125. w Vol is the amount of sample in mL to be pulled from the sample test tube and placed on the column The maximum volume of sample that can be loaded is 5 8 mL 35 Type 5 8 in the box This will cause 5 8 mL of sample to be loaded onto the column After the sample is loaded on to the column it will be sent to Aqueous waste 36 Click in the mL min box for the third line The data entered here will be the flow rate of this step Type 1 2 in this box This will set the rate at which the sample flows onto the SPE column bed at 1 2 mL min Continue writing the procedure according to the information below Enter a short description in the Procedure Description field w Create procedure using reagent names for all modules Iof x File Edit Reagents Setup Variables Password its wens S Liquid Created 3 26 98 3 39 48 PM Step Source Output Vol ml min Sense 3 1 Add to Sample H20 Cannulal5 30 No a LastModified Condition Oow 5 fe No LT Run Time DEN 3 Aaw 58 12 No 4 Procedure Description 3 I 6 P N 55078 Rev 5 RapidTrace SPE Workstation Installation Manual Zymark Corporation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 34 RapidTrace Software Purge and Clean Cannula A Purge and Clean Cannula step with water should always be the last step in your procedure as it helps prevent carryover between samples This step cl
126. which may help to remove the blockage If the blockage cannot be removed replace the syringe assembly Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Service 77 Reloading RapidTrace Firmware Intermittent or inconsistent communications between the modules and the RapidTrace Controller are an indication that it might be necessary to reload the RapidTrace firmware The following procedure will download the RapidTrace software to each of the modules The Firmware will always load to Module 0 therefore set the number switch of each module to 0 when loading the Firmware Reset each module s number switch as appropriate to ensure that no two modules have the same number assigned at any time during the downloads Example 1 2 Load the firmware to a maximum of five modules at a time If you have more than 5 modules disconnect Module 5 from Module 4 and load to Modules 0 through 4 Disconnect Module 0 from the RapidTrace Controller and connect Module 5 directly to the Controller Using the switches on the front of each module set Modules 5 through 9 to Modules 0 through 4 respectively and repeat the above download procedure for each module one module at a time Loading Firmware 1 Click on the Load RapidTrace Firmware icon in the RapidTrace folder If this icon is n
127. with a PC the beep can sound only if a sound card has been installed in the PC To set and test the beep 1 Click on Beep The following screen will appear w Beep Control i l Ea Enter number of repeats per signal E omj re 2 Highlight the number in the box then overwrite it by typing in 50 50 Click on the Test button The computer will sound the test beep representing 50 Click on OK and the screen will disappear The beeper is now set at 50 Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running Methods and Monitoring Workstation Operations 43 Running and Monitoring SPE Procedures on Up to Ten Modules This screen displays a box representing each module in a 10 module workstation Each module box has both a Run and a Stop button The screen will show which modules are active power on and attached to Controller through RS232 communication cables by showing the assigned procedures The Controller polls the modules every few seconds for updates w Run RapidTrace Beep ji Module 0 Module 1 Module 2 Module 3 test2 spe test2 spe test2 spe test2 spe test2 spe test2 spe test2 spe test2 spe test2 spe test2 spe Imlspe ImlL spe ImL spe Im spe Iml_spe Iml_spe Imlspe Iml_spe 0 1ml spe 0 1ml spe Run Stop Run Stop
128. www artisantg com 16 RapidTrace Parts and Functions External Plumbing Figure 3 External Plumbing Diagram WARNING AAAA Never use hydrogen or other flammable gases The wrong gas may explode or catch on fire Liquid exiting the solvent and sample drains can be toxic flammable and or corrosive Refer to the Material Safety Data Sheet MSDS for detailed information on a solvent or chemical Do not touch drainage Keep drainage from heat or flame Dispose of drainage properly Periodically check drainage tubes for damage Make sure that the flow is not inhibited and that the waste container is large enough to hold the drainage Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com RapidTrace Parts and Functions 17 Table 3 External Plumbing Parts and Functions Item 1 2 Part Syringe 12 port Valve Vent port on 12 port valve Gas Valve 1 mL or 3 mL Column Plunger Mixing Vessel Cannula Function Syringe and liquid sensor Directs the liquid flow from the syringe to one of the following ports as specified in the procedure e Reagents through 8 8 different ports Vent Cannula Column plunger Mixing Vessel Syringe draws air from the vent as required Activates gas drying of the column bed if the valve is plumbed into the unit and t
129. y primed by the start of the second sample If leaks are noted retighten fittings Write a procedure to test for leaks in the waste lines using appropriate reagents for specific waste lines i e aqueous organic etc While performing the test check the back of the modules for leaks in the waste fluid path and retighten fittings as necessary Create procedure using reagent names for all modules ojx File Edit Reagents Setup Variables Password Procedure Name Dga creased Sep Source Out Vat in 1 Condition Reag1 Wastel 6 42 No a LastModified 1 21 98 11 46 49 AM 2 Condition Reag2 Waste2 6 42 No 3 Condition Reag3 Waste3 6 42 No g a es ee ee ee A Procedure Description 5 ee a E A A E This procedure checks the integrity of 6 No the waste fluid paths 7 No 8 No a Nn Zymark Corporation RapidTrace SPE Workstation Installation Manual P N 55078 Rev 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Service 75 Service Gas Drying Conversion As shipped the RapidTrace modules do not have the correct plumbing arrangement to perform gas drying of the column bed To use gas drying the plumbing will need to be modified Follow the instructions below 12 PORT VALVE Remove this line from the 12 port valve to the column GAS VALVE Newer valves 2 o clock Older valves NO Attach this line

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