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Microscope SOP - St. Louis Community College

1. Student Scopes various Axiovert 25 Inverted Nikon Labophot FV BRDG R Parco XMZ Stereo BRDG STLCC CPLS Morrison 9 4 2015 February 2008 Last revision Jan 2014 Parco bulb info SM244 245 Microscope th SOP Ma X A see Projection SOP for other information e Parco LTM 800 BRDG Leica 4000DMI Fluorescent BRDG Prepared by Bob Morrison STLCC Instrumentation Specialist Page 1 SM244 and SM245 Nikon Labophot 2 Camera Control CMA View Selector Camera Am S vee Control Rod Turn on green light on ipaa Eyepiece push in bottom shelf of cart Camera pull out Player CLAIM MGE i Turn dial on selector box To DVD VCR setting Selector box Objectives 4 10 20 40x Epson remote used V P d To power on projector gt Focus amp a Fine focus Condenser Diaphragm Adjustment fii r Light Source Slide Off O gt lt Adjustment STLCC CPLS Morrison 9 4 2015 f Ee Page 2 Microscopy SM244 amp SM245 Projecting the Microscope to Screen Power on the Microscope Power on the VCR DVD device and set it to VCR mode Power on the Projector using the Epson remote Power on the white Sony CMA D2 1 x 6 box on the bottom shelf Turn the dial on the small video selector control box to DVD VCR settings Focus the specimen in the microscope eyepiece as you would for normal observations Note this requires that t
2. then Save then enter a filename and format usually jpg Close Exit the Adobe Photoshop application when finished with all captures STLCC CPLS Morrison 9 4 2015 Page 5 Microscope Inverted Image Capture Set Video Source USE 2 0 Camera X Properties Video Proc Amp age C Composite Video S Video Fator Default Cancel Snap Shot Reset VS Ds Doo USB 2 0 Camera x Cancel Snap Shot STLCC CPLS Morrison 9 4 2015 Microscope Axiovert Camera and Control Unit MTI DAGE DC 330 Camera and Control Unit CCU 10 bit digital processing Resolution up to 750 TV lines On chip integration to 8 seconds 256 frames Digital horizontal and verticle detail enhancement On screen programming for 41 internal camera functions Three user defined memories retain camera settings RS 232c interface Automatic color shading correction RGB Y C NTSC or B W video outputs Set Gain to manual to avoid auto adjustment changing scope view HotLink to DC 330 Camera Specs on a website STLCC CPLS Morrison 9 4 2015 Page 7 Microscope Axiovert 25 User Manual Fluorescent Viewing ps Axiovert 25 Axiovert 25 C Axiovert 25 CFL Ea 2 4 5 Reflected light fluorescence requires the use of the Axiovert 25 CFL microscope stand e Select a point on the specimen in transmitted light brightfield or phase contrast To do this switch the reflector mount
3. 1 products found Sort by Please select Color Lamp wattage Temperature Capbase Bulbshape W K Pro FTD 20W 12V MRIL GU4 MR11 20 3000 T Leaflet SUE CL ARS Order Code 378224 Microscope Parco LTM 800 BRDG Binocular LTM 802 P Monocular 10x WF w Pointer 4x 10x 40xR 100xR Halogen Use choke ring collar around left side course focus knob to tighten tension on stage prevent Sagging stage e Choice of 45 monocular 90 dual view video binocular or trinocular head e 10x wide field eyepiece with calibrated pointer on select models e DIN achromatic objectives are parfocaled and parcentered e Quadruple nosepiece has precision ball bearing movement with precision stops e Coaxial coarse and fine focusing with planetary reduction gear system e Positive stops at both ends of the stage to prevent damage to specimens and optics e 6V halogen illumination with two element condenser and advanced electronics e Adjustable light intensity control know e Built in mechanical stage 1 25 N A Abbe condenser iris diaphragm and blue filter e Spring loaded stage clamp for exact positioning of specimen e Tension adjustment eliminates stage drift STLCC CPLS Morrison 9 4 2015 Page 15 Pieco Z FD Serres Condenser nearly touching the slide specimen Ideally a drop of immersion oil is placed between the condenser and the slide as well as between the slide and the 100X objective Although the practice is not often followed i
4. e Interpupillary adjustment of 55 to 75mm e Dual diopter adjustments e Coated optics for crisp image and superior resolution e Illumination and stand for examining opaque or translucent specimens e Heavy duty rack and pinion focusing with slip clutch and tension adjustment e Includes a 75mm frosted stage plate and 75mm reversible black white stage e Locked on spring mounted stage clips e 120V 20W Power Supply Internal reqd for incident lighting 110VAC to 12V DC STLCC CPLS Morrison 9 4 2015 Page 12 Lines KM PREVENTIVE USE AND MAINTENANCE OF YOUR PARCO AMZ SERIES MICROSCOPE The PARCO microscope requires only minimum maintenance and has features designed to pre vent many of the accidents common to mos student microscopes These features include locked in stage clips and eyepieces eliminating easy loss and damage We have also eliminated a large percentage of annoying mechanical prob lams by locking the Rack amp Pinion Gearing Mecha nism together If forced the focus knob controlling the movement of the gears will tum freely a patented system OPTICAL PARTS The eyepiece objective condensers and reflect ing optical elements are the most delicate parts of your microscope Care should be exercised to safeguard these elements against abuse or extra rough treatment Your microscope shauld be kept covered with the PARCO cover 594 0800 when not in use This helps keep dust off optical ele ments and the gear
5. CORE o LAS Windows Icons LAS 3 8 Leica Application Suite used for most general needs LAS AF Leica Application Suite for Advanced Fluorescence Hotlink to Leica DMI Application Suite Help Manual pdf 1087 pgs Hotlink to Leica DMI LAS Installation protocol pdf 82 pgs STLCC CPLS Morrison 9 4 2015 Page 26 Microscope LeicaDMI User Interface Screen Sections Main Too Bar Come here for File Options and Help menus Workfiows Select Setup Acquire Browse Process and Analysis here Woduwe Launcher Click ta reveal the installed modules and launch them if they are enabled Contro Panels All af the power tools for the selected Vyor amp fiaw L abbedg Contro Panels Click tabs to select additional tools far the Workfiow and running module t Image Data Form Displays and edits selected data for the current image The Image Viewer Display and working area far the current image Press keyboard F5 to show full screen Side Too Bar Common working tools control all aspects of the display and tasks D Cc HH Ch amp h Phe Grid Displays the current folder image data in a scrollable grid format Available only with LAS Archives 10 he Galery Displays a thumbnail of all the images in the selected folder 11 Fast Search Controls Create filters and fast search far images Archive Option dependant 12 Gallery Browser Locate rapidly thumbnails and display in the Viewer 1
6. KMOVRadar 5 KSDKRadar amp AT amp T Family O AE A AA A AAT F B i E gt You are here Products Main Microscopy Microscope Parts and Shopping cartis empty Accessories Light Bulbs Light Bulbs LBB2 LBB3 Halogen Bulbs BROWSE OUR 2072 2074 f E HIGH SCHOOL amp LABORATORY j p EE CATALOGS Product Categories Microscopy Dissection Supplies Models Product No Description Model Series UOM Price LBB 15w 6v AMZ 832 10HF XMZ 856 10HF XMT 01BF Top Light Each 9 00 2 LBB3 20w Bv PBH 5000 SBH 6000 PBH 7000 LTM 800 RCM H Each 9 00 Measurement STLCC CPLS Morrison 9 4 2015 Page 18 Microscope Lamp 5W replacement INVOICE Page 1 of 1 Invoice Number 48401543 RI P Number 24840318 Invoice Date 05 07 13 CA RO Li N A Shipping Terms FOB SHIPPING POINT World Class Support for Science amp Math Sales Order Number 24840318 WB IMPORTANT Please Refer to the Invoice Number on All Payments Please Remit Payment To Sales Order Date 05 06 13 supply Company socom CARD Please Pay This 0 00 Payment Terms Charlotte NC 28260 0232 PAYMENT Amount Bill To 3361638 Ship To 3361638 ROBERT MORRISON ROBERT MORRISON 706 MUIRKIRK LN 706 MUIRKIRK LN MANCHESTER MO 63011 MANCHESTER MO 63011 4030 RL sipped ecko 1 EA 1 1595540 BULB FLUORESCENT 5W 10 95 10 95 STLCC CPLS Morrison 9 4 2015 Page 19 STLCC CPLS Morrison 9 4 2015 Micr
7. Axiovert Capturing Images for PC and Projector 1 Power on the Microscope Camera Control Unit MIT DAGE 2 x 5 box and the Projector Verify that the CCU box is on with green LED shown Make sure the Gain switch on the CCU is set to Manual to avoid camera attempts to rebalance brightness and thus dim your scope image Projector is controlled by Epson remote red power on button The Video button at the 6 9pm position gives control to the camera 2 Turn the View Selector Knob on the scope next to the main focus dials clockwise until it hits stop at about 4pm position this directs light toward the eyepieces Focus on specimen adjust condenser and light controls to get desired image 3 Turn the View Selector Knob counterclockwise to stop position about 10am position this directs light toward the camera and then through an adapter to the PC projector 4 Viewing and Capturing Images Start the Adobe Photoshop Program Select Start from Scratch then CANCEL the next popup menu Select File then Import then USB Camera Device If the scope image does not appear in the popup window select Image at the bottom and then select the Image tab On this menu set the input mode to S Video and_NTSC should be the default settings Adjust brightness and other parameters using slide bar controls Select Capture Still Image and verify capture in popup window Select File
8. Fluorescence Ref Figure Lamp Halogen w clipmount HLWS A 6V30W NARVA Part 000000 0402 943 have spares BRDG aL AEH BROW DB EquipmentDB rer Pis Crap Morrison 9 420 e lie Pr note gadget Notepad ica Microsoft PowerPoint 3 Febio TL Microscope Axiov e m 9 09 AM Microscope Fluorescence Typical Path and Filters dchroic mirror D uu e evo tado niter ra CN co cct wc S MC Te STLCC CPLS Morrison 9 4 2015 Page 10 Microscope Axiovert 25 BRDG Countertop sn sceses4 Condenser Light Diaphragm Adjustment Filter Guide Phase Clear Varel Note Make sure the Condenser is pulled fully forward and locked into position Light Source Adjustment Link to Zeiss Axiovert 25 CFI User Manual pdf Note This bench device does not have a camera STLCC CPLS Morrison 9 4 2015 Page 11 Microscope Stereo Dissecting BRDG Parco XMZ XMZ 833 10L Binocular 10x WF 7 5x to 35x Top Halogen Bottom LED SUPPLY OF MICROS EE e qo x pro MODEL AU 0020SB 08 AGN0 INPUT 100 240V 6 54 A5J2 47 60Hz AD e OUTPUT 12V 117A e E Nr ase S SUN PowER coRFOT P Parco XMZ series XMZ 0620131 1 25 10 RGM BS recd OK for Use 7 12 12 RGM Power supply out thus incident light does not work seeking supplier of part e Binocular head inclined at at 45 angle e Paired 10x wide field eyepieces e Working distance of 85mm
9. break off and become lodged into the socket Handle the new bulb only with tissue paper or the plastic in which itis wrapped Install the new bulb by reversing the procedure OO NOT HANDLE NEW BULB WITH BARE FINGERS Bulb may explode when heated if not handled correctly Replacement Bulb Parco 458 TOO ee REPLACEMENT FUSE To change the fuse simply unscrew the fuse cover located on the back of ihe base by turning it counter clockwise Remove the old fuse insert a new fuse and replace the cover Replacement Fuse Parco 58 7020 06 fud Parce b U 2o gx 7020 Focus Holding problem Correction 28e EG PARCO SCIENTIFIC COMPANY Inatrumant Dresinn Bso901FL Phone 800 247 2726 Fax 216 394 2403 P O Box 189 Vienna OH 44473 Page 16 Microscope Parco LTM 800 Bulb Replacement https www nterlight biz SubmitOrder int enter for Plant and fife St Louis Communit Ea STLCC FV SMET Divi W St Louis Communit BROWSE BY CATEGORY CLEARANCE BULE GUIDES CATALOGS SHIPPING INFO FLUORESCENT BALLASTS k AIRCRAFT AIRPORT AIRFIELD EULES BALLASTS BATTERIES HID k wo oc w F CELING FANS LIGHT FIXTURES LED COPIER ELILES ELECTRONICS FEATURED PRODUCTS HOME ORDER CONFIRMATION FLUORESCENT GERMICIDAL UVA UVE UVC ORDER CONFIRMATION 141988 GROW EULESS HALOGEN QUARTZ TUNGSTEN HID HIGH INTENSITY DEC HARE w F w F w F or F INCANDESCENT email I
10. dye laser tuned to 595 605 nm or less efficiently a krypton laser at 567 nm The absorption extinction coefficient at 596 nm is about 85 000 M 1cm 1 A protein with the Texas Red chromophore attached can then itself act as a fluorescent labelling agent an antibody with a fluorescent marker attached will bind to a specific antigen and then show the location of the antigens as shining spots when irradiated It is relatively bright and therefore can be used to detect even weakly expressed antigens Other molecules can be labeled by Texas Red as well e g various toxins The dye dissolves very well in water as well as other polar solvents e g Dimethylformamide acetonitrile Texas Red attached to a strand of DNA or RNA can be used as a molecular beacon for highlighting specific sequences of DNA Texas Red can be linked with another fluorophore A tandem conjugate of Texas Red with R phycoerythrin PE Texas Red is often used Fluorophores like Texas Red are commonly used in molecular biology techniques like quantitative RT PCR and cellular assays 3 STLCC CPLS Morrison 9 4 2015 Page 42 STLCC CPLS Morrison 9 4 2015 Student Microscopes examples E Pr Tu a PEPEE Revolving Nosepieer Nase plece Objectivos i Threaded into ruesepiece tue Disk Diaphragm _ Rack and Pinion Mechanism Inside Arm THluminator with Bulb line Focusing kh noh Light Switch Base Adjustment Screw Revolving nosapiece aS Multi
11. ela electromagnetic spectrum of radiation that extends from high frequency gamma rays thro _ Optical JE A rays Ultraviolet light infrared radiation and microwaves to very low frequency long Properties Working Microscopy Primer wavelength radio waves The complex phenomenon of visible light is classically discussed Tube Length w Distance TES terms of rays and wavefronts Starting with the nature af electromagnetic radiation a wide Magnification i l q Pd Color Code E variety of topics are covered in this section including retraction reflection diffraction Coverslip Brun Oncepks interference birefringence polarization primary colors human vision mirrors prisms Thickness m UL Spring Loaded Special Techniques beamsplitters laser systems geometrical optics filtration color temperature and the speg cenar af light Figure 1 Fluorescence Confocal Microscopy Anatomy of the Microscope A thorough discussion of the elements that comprise modern Digital Imaging microscones and theories behind important concepts such as mananification image formation zi LL Lorem 2 Page 53 Microscopy Other Procedures 1 Koehler Proper Specimen illumination Adjustment STLCC CPLS Morrison 9 4 2015 Place prepared slide specimen on stage center 10x objective Close down reduce light source diaphragm in base Lower condenser until diaphragm is in focus Center image using condenser cent
12. is at nm blue Therefore for fluorescence microscopy da y DAPI is excited with ultraviolet light and is detected through a blue cyan filter The emission peak is fairly broad 2 DAPI will also bind to RNA though it is not as strongly fluorescent Its emission shifts to around 500 nm when bound to RNA 3 DAPI s blue emission is convenient for microscopists who wish to use multiple fluorescent stains in a single sample There is some fluorescence overlap between DAPI and green fluorescent molecules like fluorescein and green fluorescent protein GFP but the effect of this is small Use of spectral unmixing can account for this effect if extremely precise image analysis Is required Outside of analytical fluorescence light microscopy DAPI is also popular for labeling of cell cultures to detect the DNA of contaminating mycoplasma or virus The labelled mycoplasma or virus particles in the growth medium fluoresce once stained by DAPI making them easy to detect STLCC CPLS Morrison 9 4 2015 Page 41 Microscope Fluorescence Texas Red Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye used in histology for staining cell specimens for sorting cells with fluorescent activated cell sorting machines in fluorescence microscopy applications and in immunohistochemistry 1 2 Texas Red fluoresces at about 615 nm and the peak of its absorption spectrum is at 589 nm The powder is dark purple Solutions can be excited by a
13. lens surfaces need cleaning focus the microscope on a clean slide free of all dust Moving the slide will determine if the visible dust is on the slide Rotating the eyepiece will establish if dirt is on the eyepiece After loosening the retaining screw if there is one rotate the eyepiece ina circular fashion If any dirt rotates the eyepiece needs cleaning Remove the eyepiece and clean it Be careful not to damage any pointers Clean the eyepiece on both ends in the same fashion described above for the objectives When the eyepiece is thoroughly cleaned and dried replace it and refocus the microscope STLCC CPLS Morrison 9 4 2015 Page 49 Microscopy Objective Lens Cleaning http www flinnsci com Sections Biology microscope asp Moving other parts will likewise help determine where dirt exists Dirt on mirrors can be detected by moving the mirror while looking through the microscope Rotating objectives will establish if dirt is on a specific objective Does the specific dirt move or stay when objectives are rotated Dust on a condenser lens can be detected in a similar fashion Substage condenser lenses and mirrors should be cleaned with lens paper If the lower exterior surface of an objective has been cleaned and dirt still persists it may be necessary to clean the inside surfaces of the objective To do this the objective lens should be carefully removed from its nosepiece mounting The objective lenses are threaded into the nosepiece an
14. 2 22 10 to free passage switch on the halogen lamp 2 22 2 and move the phase slider 2 22 3 to the middle position brightfield or to the position with the ring diaphragm phase contrast e Switch on the HBO 50 lamp 2 22 5 by means of the power supply unit but block the light path using the additional filter slider 2 22 6 e After selecting the specimen position cover the transmitted light beam path by means of the cover plate 2 22 11 in the Ph 3 fold slider 2 22 3 or switch off the halogen lamp e With the reflector mount 2 22 10 slide in the required filter combination 2 22 9 and release the light path by pulling the additional filter slide 2 22 6 e By means of the push rod 2 22 7 close the luminous field diaphragm until it is visible in the image move it to the middle position by means of the centring screws 2 22 8 and open it up to the edge of the field of view e Additional excitation filters with a diameter of 25 mm which must be held by means of zero rings can be inserted in the additional filter slider 2 22 6 of the reflected light unit FI The additional filter slider 2 22 6 allows no light to pass in the center stop position ap aL BRDG DB EmquipmentDB StudentDB Personalpa gt Protac ols POrive Dane gt Skat EF Moro Ta ee i note gadget Notepad c Microsoft PowerPoint 7j bio ve 2 o am L Microscope_Axiov In xl B Microscope Axiovert 25
15. 3 Gaern Thumbnail scaler Click and drag to re size the thumbnails 14 Acquire Universal capture button Status Bar Displays Hardware Configuration RGB Intensity Stage Position and Magnification data STLCC CPLS Morrison 9 4 2015 Page 27 Microscope LeicaDMI Microscope Work Flow and Functions A ques tem E amo LAS Work Flow Tabs and Function eons d 1 Setup do not use adjust system parameters preferences c eM d 2 Acquire adjust parameters on scope and camera then acquire ex ex cs image to process and or save 3 Browse setup folders and navigate for file save operations 4 Process after acquiring an image used to annotate or enhance STLCC CPLS Morrison 9 4 2015 Page 28 Microscope LeicaDMI Objective Contrast Method Illumination Controls f t o o STLCC CPLS Morrison RDK 9 4 2015 Mic1 is highlighted with an orange strip at the top this is the panel that the objectives contrast methods and illumination are controlled through Objectives are selected by manually rotating on the microscope The corresponding box will be highlighted with orange on the screen Contrast methods are chosen by clicking on either BF Brightfield PH Phase Contrast FLUO Flourescence or Page 29 Microscope LeicaDMI Camera Controls Jas EID STLCC CPLS Morrison RDK 9 4 2015 By clicking on the Camera tab at the top left this panel will become active By click
16. NFRARED Thanks again The Interlight Team LCD DLP PROJECTOR TV LAMPS Hm v Order 141988 MARINE NAVIGATION LAMPS k MINIATURE AUTOMOTIVE INDICATOR LAMPS F Bill Ta Eob Morrison 706 Muirkirk Lane NEON Manchester MO 63011 UNITED STATES MISCELLANEOUS ITEMS PROJECTION STAGE STUDIO ANSI CODE EULES 314 971 3795 bmorrison amp stlcc edu PROJECTORS RECYCLING SOCKETS ADAPTERS STARTERS k QTY ORDER CODE DESCRIPTION SPECTROPHOTOMETERS HPLC DETECTORS p STROBES FLASH TUBES 4 WW 47 2 5 64250 7388 XENON KRYPTON k STLCC CPLS Morrison 9 4 2015 Thank you for ordering from Interlight biz Your order number is 141988 m Google News Google E Emerald Coast Real Panama City I ABOUT INTERLIGHT CONTACT MY ACCOUNT Date 01 27 14 Ship To Bob Morrison 706 Muirkirk Lane Manchester MO 63011 UNITED STATES 314 971 3795 58X7020 for PARCO and others FHE ESB 6V 20W 7 85 Subtotal UPS GROUND Shipping Charge ORDER TOTAL Search PRINT THIS PAGE Below is a summary of your order This document serves as a record of your transaction You should also receive a copy of this confirmation via TOTAL 31 56 31 56 9 95 41 51 Page 17 Microscope Farco Lamp replacement LIM G00 6V 20W LBB3 parcoscientific com products main microscopy microscope parts and accessories light bulbs light bulbs Ibb2 Ibb3 gt E STLCCmain ge SMET Ez Google News Google W US Bank A Regions MidwestBank m AOL E
17. S Educational Stereomicroscopy Research Clinical Confocal Education Digital Imaging Stereos Imaging Systems FAQs MIC D Introduction to Optical Microscopy Digital Imaging and Photomicrography This treatise on Microscopy is divided into several sections that are available through the links displayed immediately to the left in the darker boxes and below In order to print the entire document you must download each link independently send the file to your printer ITEM Pers s anc put the results together al 60x Plan Apochromat Objective Virtual Microscopy 7 In the Bibliography we have included links to other works an optical microscopy and our Movie Gallery Section an Web Resources contains links to other microscopy sites an the Internet This Nosepiece Downloads material is targeted for educational purposes only and is not available to be posted on 0 Mounting Galleries remate websites either commercial or educational or distributed in any electronic format Manufacturer Thread Aberration Frequently Asked Questions Mortimer Abramowitz senior microscopist at Olympus cote Nippon JAPAN 7 Correction America Inc answers the SU mast commonly asked questions about microscopy and Lateral pian Apo Numerical photamicragraphy Magnification 60X 1 405 Aperture ADICH r Physics of Light and Color Visible light represents only a small portion of the entire Specialized 21047 WD 02 wn ALU ap
18. ase filter guide until rings are centered on each other should end up with two concentric rings STLCC CPLS Morrison 9 4 2015 Page 45 Microscope Cleaning Objectives from MicroscopeWorld website Cleaning Objectives In order to determine which of your objective lenses need cleaning take a clean blank glass slide and put it under your microscope Once the microscope is focused you should be able to move the slide and determine if the visible dust is moving with the slide or staying in the same place which means the dust is on the objective lens When using immersion oil for microscopy the oil should always be cleaned from microscope objective lenses immediately after use This can be done with a kimwipe of piece of lens paper no cleaning solutions are needed Occasionally dust may build up on the lightly oiled surface so if you wish to completely remove the oil then you must use an oil soluble solvent For the Cargille Type A or B immersion oil that we sell you can use Naptha Xylene or turpentine use very small amounts on the kimwipe Do not use water alcohol or acetone as the oil is insoluble to these solvents To remove other oily substances we recommend using the detergent called Wisk and prepare a solution of 1 part Wisk to 100 parts water If immersion oil was not cleaned off an objective after use and has harded on the objective moisten a piece of lens paper with a small amount of distilled water and hold it against the lens fo
19. b with your thumb and index finger pulling it straight out of its socket To replace the halogen bulb use the plastic wrapper the bulb is packed in to reinsert Ihe bulb into the socket NOTE Do nat touch the halogen bulb with your fingers this will sharten the life of the halogen bulb Reinstall the illuminator cover by reversing the above proce dure Focus Holding problem Correction PARCO SCIENTIFIC COMPANY kisirumeni Division aao FL Phone 800 247 2726 Fax 216 384 2403 P O Box 189 Vienna OH 44473 Microscope Bulb XMZ 833 10L Binocular 10x WF 7 5x to 35x Top Halogen Bottom LED P ld 09 O cs A xal Microscope Compatibility Mode Microsoft PowerPoint ca ER ES nix Lighti x PIR11 products Low Voltage Q fi www usa ecat lighting philips com professional lamps halogen lamps low voltage reflector mr 11 36588 cat t ProductList w e Customize Links 4 Google E STLCC College Intra E SE Louis Community Center For Plant and STLCC FW SMET Divisi Other bookmarks i a PHILIPS Lag in Reg isle United Slales Englisn sense and simplicity For consumers For professiona amp About Philips Search Q Lighting Horne Products LED Get Irepired Lig ht Community News sup port Product catalog b Professional Lamps i Halogen Lamps F Low Voltage Reflector PF HR11 Search product catalog Q Famil avere aw Products in family MRT
20. cted contrast method are marked with a triangle Immersion abjectives are marked with a FP Adjust Parfocality black drop eee d Adjust Parcentricity Objectives which have been leamed in as 2 Help combl objectives module Fine tuning are marked with a clear drop 4 Parfocality x Start Find focus 15x 20x 10 1000 Ex 500 2 5x 250 Apt 100x Firash 11506061 11506055 emmers 11506132 Colour Cancal Close Press Start to start The selected objective blinks you are changing the Each objective button shows small status icons mode trom ORY to iMMersion and vice versa The stage is lowered and you have to canfim the change 4 Marks an objective If lf is valla for the currently of made with an additional mouse click selected contrast methnod 4 Pariocality can be adjusted using the context menu of Marks immersion objectives OI Water the ngit mouse button This will startthe Parftoeality Glycennal wizard 5 Itis recommended that he Parfocal y of all listed objectives is adjusted if new objectives are Marks Comb Ohectives for use in both modes leamed in Immersion and Dry Mogel Page 38 STLCC CF Tn learn in new objectives please go ta the Workflow lll Starts the Parfocality Wizard Filter Cube TX2 ET Description Excitation Filter blue Dichromatic Mirror green Suppression Filter red Filter Set LED Wavelength Ordernumber size S Ordernumber s
21. d must be carefully removed for cleaning This should be done with the utmost care to avoid stripping the threads and or scratching the finish on the objective Apply a firm even pressure on the serrated top of the objective while holding the nosepiece from turning A padded wrench or leather strip may prevent scratching of the objectives Do not overtwist If the objectives seem too difficult to loosen with a small wrench call a microscope repair technician Clean the inside of the objective lens just like the outside try to avoid lint and dust from getting back inside the objective An aspirator is very helpful when working on the inside of an objective lens If after cleaning all surfaces carefully dirt is still found in the field of view it is possible that dirt is between the lenses of the objective This dirt cannot be removed without disassembling the compound lens in the objective Do not attempt this call your microscope repair technician Final Inspection of Objectives After cleaning it may be useful to check the overall operation by inspecting the objective lens using another microscope on a low power objective Most objective lenses extreme bottom of the lens can be removed from the objective mount by unscrewing the cover or retaining sleeve with fingers or plier tools To avoid scratching the surfaces with pliers use a cloth between the tool and the objective Put the objective lens on a clean class slide under another scope and foc
22. de Favorites de Links Js Local Setings de My Documents de Corel User Files Es Image Backup bi LAS Report k My Music de My Pictures Js My Videos jk SafeNet Sentinel de SnagltCatap LJ Meke New Folder Page 36 STLCC C Microscope LeicaDMI Setup Nosepiece Air Immersion Some objectives can be used both in air and also immersed in water or oil These so called Combi Combination objectives can be tagged so that when they are selected the user is given a warning that immersion can be an option 1 Click on the Objective Icon to select it 2 Click the check box to enable ticked or disable the Combi tag If either a tagged Combi or Immersion only objective is selected on the Acquire gt Mic 1 tab the button will flash 3 Immersion only objectives are marked with a filled to warn the user teardrop icon whereas 4 Combi objectives are marked with an outlined teardrop Nosepiece NOSEPIECE amp POS pm Fine Tuning Configuration Objective Tune Dry and immersion 2 5x Sx 10x 20x 40x 63x 11506083 11506087 11506088 11506096 11506099 11506100 STLCC CPLS am 37 Microscope LeicaDMI Nosepiece Control Dry Immersion 1 All learned in objectives are displayed in the control window 2 The current objective in the light path is highlighted an the control 1 Objectives 10 x Objectives which are valid for the sele
23. e when not in proper working condi tion PARCO STANDS TO BE OF SERVICE TO YOU Microscope Parco XMZ Series Instruction Manual CORRECTING BASIC NECHANICAL PROBLEMS DRIFTING If the focus block of your microscope falls by the weight of gravity and will not stay in a facused position itis said to be drifting It is the result of loss of tersion in the pinion mechanism due to normal and constantuse he tension is easily and quickly adjusted You need not employ the ser vices of a microscope technician to perform this function To correct for drifting immediately to the right tha left focus knob there is a pinion tension adjust ment ring Turn the adjustment ring in clockwise motion in relation to tha focus knob A 1 4 to 1 2 turn is all that should be necessary REPLACING ILLUMINATOR BULBS To replace the bottom bulb on the stand Unplug the line card Wait until the bulb is cooled Turn the microscope on its side and remove the three screws located on the outer edge which hold the base cover on Remove the old bulb by pulling it straight out of its socket You will feel it release from its mount Install the new bulb by reversing the above procedure To replace the top bulb on the stand Unplug the line cord Wait until the bulb has cooled While holding the cover to prevent it from falling remove the two screws which hold the illummator cover on by turning tiem counterclockwise Grasp the halo gen bul
24. en Dichroic GU4 30 degree 35mmO Page 21 Microscope Student Leica CM E Link to Microscope Educational Materials On Florida State University Website The Leica S4 E with 4 8 1 zoom and standard magnification of 6 3x 30x is the basic model of the Leica StereoZoom line 4 8 1 zoom Standard magnification 6 3x 30x Overall ergonomic design and comfortable 38 viewing angle Largest field of view of any instrument in its class 36 5mm Working distance 110mm The Leica StereoZoom range offers a flat planar field of view polymer which makes this combination perfect for electrostatically sensitive work STLCC CPLS Morrison 9 4 2015 raye lt lt Microscope Oil Immersion Leica ATC2000 Link to Basic Leica ATC instruction Manual pdf Link to Oil Immersion Guide from Clermont College Website htm STLCC CPLS Morrison 9 4 2015 Page 23 Microscope Local Services Repairs Bulb Replacement Bulb Replacement Orders Per GN 3 31 11 Archway Lighting Supply Incorporated 314 535 1314 2739 Washington Ave St Louis MO 63103 Call or Go to the archway website http www bfmgraphics com al major_manufacturing htm Go to the products section or any area and select the phillips hotlink On the Phillips site select product then professional lighting http www Ecat Lighting Philips Com I professional lamps ep01 gr us lp prof atg cat us Omnpg lamps professional amp lptype lamps amp navaction pop amp navcou
25. ering screws Open diaphragm to edge of field fine focus and open further to just clear field Adjust contrast using condenser diaphragm Remove eyepiece and check that 75 of visible aperture is filled with light Page 54
26. he View Selector Rod blue on diagram is in the full in position to deflect light toward the eyepieces eos SS rm Pull the View Selector Rod blue box on diaqram on the riqht side of the microscope frame supporting the eyepiece to the out position This detflects light from the eyepiece to the camera If you still see any light in the eyepieces pull the View rod to the full out position When finished with the camera projector push the View rod to the in position to resume using the eyepiece for adjustments or another specimen Note If the projected view dims or adjust automatically to an unsatisfactory image adjustments can be made on the camera Auto Exposure modes see slide 7 A setting to manual mode is often effective Link to SM 244 and SM245 Computer Projection Instructions pdf STLCC CPLS Morrison 9 4 2015 Page 3 la ep 224 T or Je MINOR PE To Projector PC Monitor Svideo to USB box MTI DAGE Camera ControlUnit CCU FP i ETE P W green indicator STLCC CPLS Morrison 9 4 2015 Off On Microscope Axiovert 25 O BRDG R124 sicc 0097770 Condenser Light Shunt Filter Guide Phase Clear Varel Condenser Light Diaphragm 3 Light Source Adjust Reflector Modules Filters Fluorescence View Selector Knob Eyepiece clockwise Fou Projection ccw Fine focus Microscopy
27. ices Repairs Bulb Replacement Bulb Replacement Orders Per GN 3 31 11 Archway Lighting Supply Incorporated 314 535 1314 2739 Washington Ave St Louis MO 63103 Call or Go to the archway website http www bfmgraphics com al major_manufacturing htm Go to the products section or any area and select the phillips hotlink On the Phillips site select product then professional lighting http www Ecat Lighting Philips Com I professional lamps ep01 gr us lp prof atg cat us Omnpg lamps professional amp lptype lamps amp navaction pop amp navcount 0 amp omnpc ep01 gr us lp prof atg amp isleftna v false Locate type of bulb compact fluorescent halogen From Naumann Virginia L Sent Thursday July 12 2012 9 35 AM Subject RE Microscope repair services local Hitchfel 2333 S Hanley Road St Louis Missouri 63144 T 800 242 3501 Spakowski Microscope Service 7739 Brookline Ter Saint Louis MO 63117 T 314 644 6560 STLCC CPLS Morrison 9 4 2015 Page 52 Microscopy Education Tutorials FAQ http www olympusmicro com primer index html E Olympus Microscopy Resource Center Introduction to Microscopy Microsoft Internet Explorer o x File Edit wiew Favorites Tools Help Q Back gt x 2 A a Search yf Favorites Cr iw Lj rel Address http fs olympusmicro com primer finde Atm Links Customize Links Free Hotmail E Windows w windows Marketplace Windows Media OLYMPU
28. ield diaphragm lens Adjust the condenser aperture setting to give the best image 1 Change objectives to produce different total magnifications Remember that your total magnification is eyepiece magnification 10x times the objective lens magnification STLCC CPLS Morrison 9 4 2015 Page 47 SET UP A STEREO DISSECTING MICROSCOPE TO BE PARFOCAL written by Nancy Kruger Feb 2008 1 Adjust the eyepieces oculars so they are at the midpoint setting indicated by a line or matching a dot to a line Adjust the eyepiece spread to match the distance between your eyes 1 Place a specimen on the stage with the appropriate illumination top episcopic or transmitted diascopic illumination or both 1 Using the lowest magnification setting focus on a distinct area of the specimen Use the coarse focus knobs to set the focus 1 Change to the highest magnification using the magnification set knob Use the coarse focus knobs to adjust to the best focus 1 Change back to the lowest magnification using the magnification set knob DO NOT TOUCH THE COARSE FOCUS KNOBS 1 Block off your left eye using your hand or piece of paper Adjust the right eyepiece to produce the best focus possible for you by twisting the top portion Switch eyes and repeat the process 1 Check your settings by zooming between the lowest and highest magnifications The image should stay in focus over the entire range If not repeat the process You may need mi
29. ing mechanism Dirt settling in the gears causes excessive wear and should be kept clean to prolong the life of your instrument CLEANING THE OPTICS When specks or smears appear in the field of view the optics need cleaned If the specks move when rotating the eyepiece clean the top of the eye piece The front lens of the objective should be cleaned by first brushing with a soft camel hair brush lens paper or clean cotton cloth to remove dust particles If this does not remove the smudge moisten lens tissue PARCO 63B 4005 with a good lens cleaner PARCO 63B 3999 and dry with clean lens tissue at once Dry with soft circular motion Do not take the objectives apart this should only be done by a qualified PARCO serviceman CLEANING THE FINISH ON THE MICROSCOPE The finish af the microscope is hard epoxy and is acid resistant It is extremely durable and stands up well under rough use Use a soft cotton cloth to wipe clean When cleaning the frame exercise care not to smear the optics MECHANICAL The focusing mechanism of the PARCO micro scope should be removed periodically once a year and slidaways lubricated with a thin film of Plastilube Before lubricating remove old film and clean slideways thoroughly This should be done by a PARCO serviceman Se eres It is in you own best interest to have your micro scope serviced at least every two years by a trained PAHCO serviceman A microscope has very little valu
30. ing on any of the triangles at the right edge of the boxes you may manipulate various camera settings Page 30 Microscope LeicaDMI Acquire Save as Dialog By clicking on the acquire screen button you will capture whatever you see on the live image to the right A save as dialogue box will appear S Seve s G se de gt Computer OSIC Users Leica Desktop P Organize New folder Ww Fovcertes s HE Desktce B Downloads be Recent Places Lbranes Documents a Muix i Pretures Videos M Computer E OS C ca CODEMETER E File nme TOEMERPETOE Save as type JPG Hide Folders Cancel STLCC CPLS Morrison RDK 9 4 2015 Page 31 Microscope LeicaDMI Camera Acquire Screen Fe Op tee 9 x Acquire Options EL Basic Functions Auto adjust white balance o a elect arrow to expand drop down menus for adjustments to camera and resulting image et CI CZ CZ CITE a COX cs CT cs STLCC CPLS Morrison 9 4 2015 Page 32 Microscope LeicaDMI Acquire Image Save As Browse or Acquire Image Leads to normal Windows panel To define folders and or filenames for images and metadata Note metadata is saved automatically with images for future Reference and documentation Images can be moved to other PCs Laptops where the LAS software has been installed for further clarity adjustment
31. ize K Filter Cube A4 ET Description Excitation Filter blue Dichromatic Mirror green Suppression Filter red Filter Set LED Wavelength Ordernumber size S Ordernumber size K STLCC CPLS Morrison 9 4 2015 Filter system TX 2 for Texas Red green excitation excitation filter BP 560 40 dichromatic mirror 595 suppression filter BF 645 75 BP 560 40 595 BP 645 75 11504180 11504170 Filter system A4 for UV excitation excitation filter BP 360 40 dichromatic mirror 400 suppression filter BP 470 40 BP 360 40 400 BP 470 40 365 11504181 11504162 Microscope LeicaDMI4000 Filter Cubes Page 39 Microscope LeicaDMI4000 Filter Cubes cont Filter Cube L5 ET Description Excitation Filter blue Dichromatic Mirror green Suppression Filter red Filter Set LED Wavelength Ordernumber size S Ordernumber size K STLCC CPLS Morrison 9 4 2015 Filter system L5 for blue excitation excitation filter BP 480 40 dichromatic mirror 505 suppression filter 527 30 BP 480 40 505 527 30 11504176 11504166 Page 40 Microscope Fluorescence DAPI DAPI or 4 6 diamidino 2 phenylindole is a fluorescent stain that binds strongly to A T rich regions in DNA 1 4 When bound to double stranded DNA DAPI has an absorption Os f M ONU maximum at a wavelength of 358 nm ultraviolet and its emission GRASS 5 IE maximum is at 461 nm blue Therefore for f CLONE SY maximum
32. n routine study itis the only way to take full advantage of the inherent resolutions of the 1 25 N A condenser I USING THE FOCUS STOP The vertical travel of the stage can be restricted by the auto focus stop Located immediately to the right of the left focus knob is a focus stop lever Elevate the stage to the point af maximum desired travel then engage the stop by turning the lever firmly in clockwise direction To disengage the stop rotate the laver counterciock WIS J USING THE TENSION CONTROL The tension control is provided to allow the individual user to adjust the focus tension to hig her own prefer ence Located immediately to the lett of the right focus knob there is a tension control ring to increase the tension Turn the tension control ring clockwise to decrease it tum the tension control ring counterclock wise PREVENTIVE USE AND MAINTENANCE OF YOUR PARCO LTM 800 MICROSCOPE The PARCO microscope requires only minimum main tenance and has features designed to prevent many of the accidents common to mos student microscopes OPTICAL PARTS The eyepiece objective condensers and reflecting optical elements are the most delicate parts of your microscope Care should be exercised to safeguard these elements against abuse or extra rough treatment Your microscope should be kept covered with the PARCO cover 4584 0334 when nat in use This helps keen dust off optical elements and the gsang mecha nism Dir
33. nor focus adjustments to see detailed structures at various planes but no major focus adjustments STLCC CPLS Morrison 9 4 2015 Page 48 Microscopy Optical Maintenance Lens Cleaning http www flinnsci com Sections Biology microscope asp 1 All lenses are made of coated soft glass and can be easily scratched Lenses should be treated with care Never use a hard instrument such as a dissecting needle etc or abrasive to clean a lens 2 For the top of the eyepiece and the ends of the objectives clean as follows Use a camel s hair brush and an aspirator to remove all loose dust and dirt Then moisten the end of a Q tip with lens cleaning solution Keep the other end of the Q tip dry Clean the optical surface with the moist end of the Q tip using a circular motion Dry the surface with the dry end of the Q tip using a circular motion Use an aspirator or similar air source to remove any lingering dirt particles 3 Immersion oil should always be wiped from all surfaces immediately after use In the event immersion oil is allowed to harden moisten a piece of lens paper with a small amount of xylene and use this to redissolve and remove the hardened oil Note Xylene may leave a film on the lens and may dissolve the cement used to seal the immersion objective To prevent this always moisten a second lens paper with alcohol and use it to remove any residual xylene Repeated use of xylene will destroy lens coatings 4 To determine which
34. nt 0 amp omnpc ep01 gr us lp prof atg amp isleftna v false Locate type of bulb compact fluorescent halogen From Naumann Virginia L Sent Thursday July 12 2012 9 35 AM Subject RE Microscope repair services local Hitchfel 2333 S Hanley Road St Louis Missouri 63144 T 800 242 3501 Spakowski Microscope Service 7739 Brookline Ter Saint Louis MO 63117 T 314 644 6560 STLCC CPLS Morrison 9 4 2015 Page 24 Microscope Leica DMI4000B BRDG Basic Startup The Leica DMI4000 B automated inverted research microscope is ideal for scanning cell and tissue cultures The system features a fluorescence axis for ultra brilliant fluorescence imaging The Leica Application Suite LAS manages the system 4 Leica Application Suite LAS 2 Scope Software app Power x on off 7 Advanced Fluorescence app 3 UV Source on off opt Pushrod In to Eyepiece Out to PC STLC e 25 Microscope LeicaDMI Startup Process Turn on Scope Top white box at left using on off green toggle switch Turn on PC to boot up Login to Windows password z microscope and wait for application screen Focus specimen using eyepiece with side pushrod on scope pushed in toward base Select LAS 3 8 for most applications brightfield phase contrast basic fluorescence Select LAS AF Advance Fluorescence for advanced fluorescence Pull eyepiece external rod out to shift image to computer LAS application
35. oo sensilive to dust lt is wise nonetheless to keep these paris clean as all dirt films in the light pathway will affect resoluton A soft lint free cloth is satisfactory It is in you own best interest to have your microscope serviced al least every two years by a trained PARCO serviceman A microscope has very little value when not in proper working condition PARCO STANDS TO BE OF SERVICE TO YOU CORRECTING BASIC MECHANICAL PROBLEMS DRIFTING If the stage of your microscope falla by the weight of gravity and will not stay in a focused position it is said to be drifing It is the result of loss of tension in the pinion mechanism due to normal and constant use The tension s easily and quickly adjusted You need not employ the senices of a microscope technician to perform this function To correc for drifting immediately to the left of the right focus knoe there is a tension adjustment ring Turn the adjustment ring in a clockwise motion in relation to the lacus kncb A 1 4 to 1 2 tum is all that should be Necessary REPLACING ILLUMINATOR BULBS Unplug tha line cord Wait until the bulb is cooled Lay the microscope on its side and remove the bulb access door by tuming the lock screw counterclockwise NOTE On binocular and trinocular microscopes remove the eyepieces as they could fall aut and get damaged when ihe microscope is tipped Hemove the old bulb by pulling it out of its socket DO NOT TWIST as the lamp pins may
36. oscope Student Leica DM750 New FV 11 11 09 M1 M27 Designated for Microlab Microscope for university advanced life science courses Key Features Focusable or fixed eyepieces Field of view of 20mm 45 degree tube Wear resistant Energy saving 4 10 40 1000 l Objectives Standard condenser for magnifications 4x 100x Phase turret condenser for brightfield and phase contrast Flip top condenser for low magnifications DM750 is available with a 4 position or 5 position nosepiece Integrated vertical handle provides easy carrying and easy lifting when storing on high shelves Integrated cord wrap eliminates damage to microscope componenis Vertical cord insertion prevents the cord from pulling partially out of the stand while in storage or in use Unique shape of the microscope stand protects controls from damage when microscopes are stored side by side Page 20 A rear facing nosepiece provides comfortable operation Spring Loaded high magnification objectives A built in blue filter to prevent filter loss 360 rotatable 45 viewing bodies Graduated mechanical stage with Vernier scales provides precise control Low heat output to provide comfortable viewing and prevent injury Tungsten Halogen lamp 20W 6V 2 000 hours life Design to meet international safety standards 120VA 220 240 Substage rack and pinion condenser STLCC CPLS Morrison 9 4 2015 icroscope Student Leica S4E Bulb KANDOlite MR11 6V 15W Hallog
37. ple obiectives HLY lis diaphragm Jever Condenser Coarse adjustment knob Light source Base Page 43 student Microscope Typical fs A Iris Diaphragm lever 39 Oculars 9am closed Y 12noon open S NV ne Revolving Aesepiece ee iple obiecbves a m a kis diaphragm lage i Stage manipulator Coarse adjustment knob Light source STLCC CPLS Morrison 9 4 2015 Page 44 Microscopes Cleaning and Phase Filter Procedures 1 Cleaning Objectives Filters Lens Remove objective use ring not objectives to rotate for removal Rub lightly with lens tissue first Remove an eyepiece and hold at 45 degree angle from objective to inspect for dust or other materials Use Cotton swaps or Q tips and Ethyl Alcohol 90 95 rub from center out in a spiral motion rotate and replace swap as needed when stained or fluffy Carl s bottle is in lab setup area Check cleaning with a prepared standard slide ex Diatoms specimen slide cover down always toward the objectives 2 Phase ring filter alignment Make sure condenser and phase rinq slot is pulled fully forward and in locked position Remove filter guide and clean filters if needed Insert a clean prepared slide with no stain Adjust condenser light intensity to lower setting to avoid glare Remove right eyepiece entirely observe Phase filter with respect to circular ring of normal objective Adjust end screws on Ph
38. r a few seconds to dissolve the oil If that does not work try alcohol Isopropyl alcohol is one of the best solvents but it must be at least 9096 pure do not use rubbing alcohol 3096 water Everclear which is grain alcohol you must be 21 can also be used but it doesn t do as well in dissolving crud If you have something like Balsam stuck on the lens you must resort to a stronger solvent like Acetone or Xylene Acetone should never be put on plastic parts as it will dissolve most paints and plastic After using solvents be sure to clean the objective again with standard distilled water to ensure that you have removed all the solvents from the microscope objective STLCC CPLS Morrison 9 4 2015 Page 46 ALIGNMENT OF A COMPOUND UPRIGHT MICROSCOPE written by Nancy Kruger Feb 2008 1 Adjust the eyepieces oculars so they are at the midpoint setting indicated by a line or matching a dot to a line Adjust the eyepiece spread to match the distance between your eyes 1 Place a slide on the stage and focus on it with the 10x objective 1 Close the field diaphragm lens on the base of the microscope if that adjustment can be made 1 Adjust the condenser height with the condenser knob until you see a distinct polygon in the field of view 1 Using the condenser centering knobs below the stage center the bright area in the field of view By opening up the field lens slightly you can fine tune the centering 1 Completely open the f
39. s annotation and or analysis 9 M BM STLCC CPLS Morrison 9 4 2015 Page 33 Microscope LeicaDMI Process Image eee fe E t Process Options T J A Select Arrows in upper right corner of each panel to produce F Drop down menus with adjustment slider bars etc l a E i UL CIE 4 m s PAL y log gt s a eh p T md a i men as LE J s ve V ENADE A45 T rc rev e Page 34 STLCC CPLS Morrison 9 4 2015 Microscope LeicaDMI Process Image Apply to Save AS Cv eee tte A H j id E APPLY hmmm After changes or annotation to raw image you must select APPLY to save those changes with the original raw image file STLCC CPLS Morrison 9 4 2015 Page 35 Microscope LiecaDMI Export Images 1 If necessary click to select the Fie option Export Images On The image Destination panel Image Destination 2 To change the destination folder click on E B O Fie the Browse for Folder button and mm navigate to the destination folder Create a new folder if required 4 Select Export Folder x Click the O button CAUsersiMy Documentsilmage Backup amp To include all ofthe Meta Data with the B B Include all Meta files Image click to enable the include all reta EEE files check box Fienal Browse For Folder x E Continued A 891 w de Downloads
40. t settling in the gears causes excessive wear and should be kept clean to prolong the life of your instrument CLEANING THE OPTICS When specks or smears appear in the held of view the optics need cleaned If the specks move when rotating the eyepiece clean the top of the eyepiece H the specks move when the slide is moved clean the cover glass of the slide The front lens of the abjective should be cleaned by first brushing with a soft camel hair brush lens paper or clean cotton cloth t remove dust par tides If this does not remove the smudge moisten lens tissue PARCO 363 4005 with a qood lens cleaner PARCO 263 3999 and dry with clean lens tissue at once Dry with soft circular motion Do not take the objectives apart this should only be done by a qualified PARCO serviceman CLEANING THE FINISH ON THE MICROSCOPE The finish of the microscope is hard epoxy and is acid resistant It is extremely durable and stands up well under rough use Use a soft cotton cloth to wipe clean When cleaning the frame exercise care not lo smear tha optics Microscope Parco LTM 800 Series Instruction Manual MECHANICAL The focusing mechanism of the PARCO microscope should be removed periodically once a year and slideway lubricated with a thin film af Plastilube Before lubricating remove old film and clean slideways thor oughly This should be done by a PARCO serviceman The mirror in base lluminator and condenser are not t
41. us to inspect for dust spots cracked lenses or other debris Morrison Mar 2008 STLCC CPLS Morrison 9 4 2015 Page 50 Microscopy Mechanical Adjustments http www flinnsci com Sections Biology microscope asp Nosepiece Adjustment The nosepiece can likewise become too loose or too tight There is usually an adjustment mechanism on the nosepiece It is often as simple as loosening or tightening the slot headed screw in the middle of the nosepiece Sometimes there is a two hole ring nut This requires using a round nose pliers like a wrench to loosen or tighten the collar On some microscopes the stage must be removed to gain access to the nosepiece adjustment Be sure to check the manual for your specific microscope Focus Knob Adjustment Tension of the coarse and fine adjustment knobs can be adjusted Again various mechanical methods have been designed Some microscopes are adjusted by simply gt WRENCH fer i i E tension control turning the knobs on each side of the microscope in opposite directions to tighten or LI loosen as desired Others have adjustable collars on the shaft and require the use of C7 specially designed collar wrenches or allen wrenches to make the adjustments Moving the collars out usually provides more tension If your microscope requires unique collar wrenches obtain these from your microscope supplier STLCC CPLS Morrison 9 4 2015 Page 51 Microscope Local Serv

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