PCR英文说明书-有step down功能
Contents
1. Cycles i gt s a i a ws o Yen Le m w i gt E a o 7 Ba ia va p t M ns ns sn Br Tia pA un pm o ai p 7 i u Br in _ u pt D PR Bu d W Bu pm n u eo b el a u d ka am T x Fig 3 Front View 1 Touch Screen 2 Rotary Knob 3 Heated Lid step 0 0 Kien Ss Num X10 Be LC waiting L Fig 4 PROGRAM page screen after completion 1 Home Key 2 OK Key Run a program Once a program is selected the screen shows the PROG page see Select a program and the heating lid start to wrok By tapping the START key 7 in Fig 2 on the screen the thermal cycling program starts and the program shows the waiting mode until the heated lid reaches 85 C The horizontal line flashes when the current segment is running After completion of a program both the HOME 1 in Fig 4 and OK keys 2 in Fig 4 appear on the screen Tap OK key to terminate the run Or tap HOME key to return the MENU page without saving the changes of the parameters Notice that the block automatically cools down to 8 C until new program Starts Pause and force quit a program At any time during a program one can tap the key to pause the program The block tempera2. T Heater 4x4 mini Cycler User Manual ver 2 0 en www coyotebio com Contents of Package One T Heater 4x4 mini cycler One 12 V DC power supply adaptor One User Manual One pack of PCR tubes For mobile applications where AC electricity is not available an optional rechargeable battery pack or 12 V car battery adapter cable can be requested from your dealer or from us Specifications Temperature Range 8 99 C Time Range 1 sec to 60 min Maintains at 8 C after the program completes Ramping Speed Average ramp rates are 2 0 C sec with a maximum rate of 3 C sec Temperature precision 0 2 C Display precision 0 1 C Sample block temperature evenness 0 5 C Temperature of heated lid 99 C Operation ambient temperature 8 30 C Sample capacity 16 x 0 2mL PCR tubes Memory 15 programs stored in the front page Power Supply 12V DC Control Panel and Display LCD touch screen and rotary knob Dimensions 200x200x40 mm Net Weight 2 5 kg Quality Assurance The unit carries a one year warranty against manufacturing defects and other problems under normal operation conditions T Heater 4x4 mini cycler is compact and easy to use It is particularly useful as a personal device for PCR small number of Samples at one time It is suitable for mobile applications Start up Connect the unit to 12 V DC power adaptor and plug in the adaptor to the wall outlet Upon connect
3. ing to 12 V DC switch on the device and the unit enters MENU page Select a program Up to fifteen PCR programs can be stored in the unit The programs are alphabetically ordered A thru O The first 5 programs A thru E are shown in the first MENU page screen The next 5 programs are in the second MENU page By tapping the 1 key one can toggle between the three pages shown in Fig 1 The thermal cycle parameters namely the temperature and the duration of each step 2 in Fig 1 and the number of cycles 3 in Fig 1 are shown in the MENU page To select a program to edit or to run just tap the area of the screen where the parameters of the program are shown Upon tapping the PROG page appears Edit a program To edit a program first enter its PROG page see Select a program In its PROG page select the parameter to be adjusted by touch the parameter value 1 in Fig 1 Once the parameter is highlighted the color changes from red to white adjust the value by turning the rotary knob clockwise to increase or counterclockwise to decrease until the desired value is reached Leaving the knob unchanged for a second the thermal profile graph display is readjusted to show the new value Repeat above steps to edit another parameter To return to MENU page tap 1 key on the screen Upon leaving PROG page a reminder appears SAVE If one taps No the new changes is lost If Yes is tap
4. ped the new program is saved Graphic Display The thermal profile of a program is shown graphically in its PROG page The parameters of each segment are shown on each side of the segment 1 2 in Fig 2 The segments within the bracket are to be cycled and the number of cycles are shown on the upright corner of the bracket 3 in Fig 2 The segment outside the bracket do not cycle The current temperature of the sample blocks No 5 in Fig 2 and the heated lid temperature No 4 in Fig 2 are shown on the lower left Heat Lid The heated lid can be turned on off by tapping it to white gray While entering the PROGRAM page see Fig 2 the heated lid starts heating When the heated lid is turned on the program starts to run when the heated lid reaches 85 C When the program completes it automatically goes to 8 C and the heated lid is turned off It is recommended to turn off the heated lid when the setting temperature is below ambient Otherwise the heated lid has a effect on the upper side of the sample and brings temperature drift to the setting point Fig 1 MENU page screen 1 Program Number 2 Parameter Value 3 Cycle Number 4 Next Page Fig 2 PROGRAM page screen 1 Parameter Value 2 Cycle Bracket 3 Cycle Number 4 Temperature of The Heated Lid 5 Current Temperature of Block 6 Back Key 7 START Key 8 Temperature Decrement of Each Step 9 Maximum Initial Temperature of the first Step 10 Number of Step Down
5. utre remains at the current temperature see 5 in Fig 2 and the timer stops counting To resume the run tap the START key To force quit a program and return to MENU page pause the program first 66 JI and then tap the key to stop the program Step Down Three parameters can be set up to start Step Down function Press step 8 in Fig 2 to set temperature decrement minimum 0 5 C of each step When step displays 0 it means no Step Down function is selected Press MTem to set the maximum temperature for the first step cycle Press Num to set the number of steps Cautions The unit should avoid contacting with strong acid or alkaline and most of the organic solvents In case of accident contact clean the chemicals immediately Salt solutions also slowly corrode metal parts and therefore should be avoided as well The unit is not water resistant Absolutely no immersion of the unit in water Only use the provided electric power adaptor provided using a different power adaptor may cause permanent damage to the device Contact Us Coyote Bioscience Company Rm 504 5th Floor Chuangye Zhonglu 36 Haidian District Beijing China Tel 86 10 62977520 Fax 86 10 64844237 E mail sales coyotebio com
Download Pdf Manuals
Related Search