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1. Note The functions of some displays are different when particular auxiliary functions are actuated Refer to Para 6 8 Aux Func 6 6 3 Functions of Keys When this key is pressed once an item to the right of the display section can be set FLOW PRESS P MAX gt P MIN gt AUX FUNC Contrary to the key anitem to the left of the display section can be set by press ing this key once AUX FUNC FLOW PRESS P MAX P MIN CE e When this key is pressed control waits for input of FLOW PRESS e Data input so far are cleared by pressing this key when values are being input e Display is cleared and the audible alarm is silenced by pressing this key when an error is displayed Data are loaded by pressing this key after keying in a value Pump is turned on or off by pressing this key and off or on by another pressing Upper left display light comes on when the pump is turned on PROG RUN Time program starts to run by pressing this key Upper left display light comes on while the program is running Program is not executed if the contents of the program are not set Run of a program can be stopped forcedly by pressing this button after program run is started 28 6 6 4 Setting Procedure The figures of the FLOW PRESS display flash when the power supply is switched on This means that a flow rate ora pressure in the constant pressure liquid transfer mode can be keyed in with the numeric keys
2. O PROG SET Used for displaying or setting time program 4 COND Diagnose pressure wave form at liquid transfer and displays results of diagnosis O SV Used for opening or closing FCV 130AL solenoid valve option EVENT Sets relay contact output and FCV 130AL cleaning pump 3 EXT S Start time program by EVENT 1 output signal 3 MONIT TIME Displays time in execution of time program 3 MONIT REV Displays integrated number of pump revolutions MONIT PLNG Displays plunger displacement MONIT FLOW Displays flow rate of liquid in constant liquid transfer mode MONIT COMP Displays value in proportion to compression ratio used for correction COMP Sets compression ratio D DIAG Check memory Caution Parameters program etc are erased HOME ADJ Used for adjusting home position of plunger FLOW ADJ Used for adjusting flow rate correction factor KCOMP Parameter for adjusting acceleration section length in pulsation correction K REV Parameter for changing motor speed in proportion to pressure LOCAL Enables pump side flow setting when system controller SCL 8A is connected to unit CLOSE KEY Disables input by every key Classifications of Displays in Table 6 8 1 The displayed function is carried out by pressing the ENTER key O The displayed function mode is set up by pressing the ENTER key 3 A value can be input when the display is shown SA value is
3. Fig 5 5 Remote Connector Wiring 1 The reed relay contact output signal corresponding to the auxiliary function EVENT lis connected across the terminals No 1 and 2 of the connector If EVENT lis selected the relay is closed If the auxiliary function EXT S is selected these terminals can be used for output ting a signal synchronized with the start of the time program Refer to Para 6 8 Auxiliary Functions The reed relay contact output signal corresponding to EVENT 2 is connected across terminals No 3 and 4 The ratings of those relay contacts are 3VA 50V 0 1A To drive external devices exceeding the rated capacities of the relay contacts connect power relays to those terminals for driving the power relays by the reed relay contacts and driving the external devices in turn by the power relays 2 External on off control Of the pump is made through terminals No 5 and 7 The pump is turned on by connecting terminals No 5 and 7 electrically with e g relay contacts The pump is turned off by opening the terminals 19 3 The time program can be started externally through terminals No 6 and 7 The program is started by connecting the terminals No 6 and 7 electrically for about 1 second with e g relay contacts etc To stop the run of the program input the stop command or press the PROG RUNIkey on the panel 4 Pressure can be recorded by connecting a 1 mV full scale recorder across terminals No 8 an
4. 21 4 Turn on the power supply switch Check the following points a Pressure indicated on the display is 0 to 5 The pressure zero point adjusting procedure is outlined in Para 5 1 b The maximum pressure setting P MAX kg cm and the minimum pressure setting P MIN kg cm are adequate The method for pressure limiter settings is outlined in Para 6 6 5 Open the drain valve 6 Press the upper right keys lo and Setting a flow rate of 40 m2 min Be sure that the left end figures of the display are 40 0 7 Press the key The pump will start up When the PUMP key is pressed for the first time after the power supply switch is turned on the pump turns slowly for a while and erp mme then speeds up 8 Look atthe condition of the liquid running out of the drain pipe for about 30 seconds It is satisfactory if the liquid flows continuously Note If air is carried by the liquid wait till air in the liquid disappears Refer to Para 9 if air is still carried by the liquid after five minutes t Comply with the steps outlined in Para 6 3 if the liquid does not carry air but flows and stops synchronized with the mechanical operating sound of the pump 9 Press the key for turning off the pump 10 Seta desired flow rate For instance key in and ENTER for setting 100 m2 min 11 Close the drain valve Caution Close the drain valve when liqui
5. completing the setting Display 50 0 0 150 FLOW PRESS PRESSURE P MAX i Flashing On completion of setting as outlined above press the key and liquid transfer begins Liquid transfer is stopped by another pressing of the key This key is always workable even if some setting is being made When the display FLOW PRESS is flashing a flow rate or a pressure can be set as desired regardless of whether or not the pump is transferring liquid This is also the case with the displays P MAX and P MIN The setting ranges of the parameters are shown in Table 6 6 1 30 Table 6 6 1 Parameter Setting Ranges Parameter SettingRange Minimum Unit initial Settine FLOW 0 150m min 0 1 m min 0 0 kgf cm pmax 0 s20kgf em 1kgticem 100kgf em P MIN 0 300 kgf cm 1 kgf cm 0 kgf cm 6 6 5 Upper Pressure Limiter P MAX If the delivery pressure of the pump exceeds the P MAX setting liquid transfer is automati cally turned off for protecting the column and other flow line parts When the limiter is actuated the audible alarm is signalled and the following message is displayed ERROR P MAX Maximum operating pressures of the unit are given according to flow rates as shown in Table 6 6 2 If the maximum operating pressures are exceeded liquid transfer is auto matically stopped regardless of the P MAX setting Table 6 6 2 Maximum Operating Pressures Flow Rate Ran
6. If the key is pressed the display next to the right of the flashing display begins to flash and the display stops flashing indicating that the parameter of the flashing display can be input If a value is input control automatically returns to the waiting condition for the input of FLOW PRESS Press the key for returning control to the FLOW PRESS input waiting condition without inputting a value The unit is normally operated when the displays shown in Fig 6 6 1 are indicated Auxiliary functions however are provided for special control The details are given in Para 6 8 AUX FUNC Setting Examples Example 1 Settinga flow rate of 50 m2 min Display 0 00 0 100 FLOW PRESS PRESSURE P MAX Flashing Key in and ENTER The display will change as illustrated below and the setting is completed Display 50 0 0 100 0 FLOW PRESS PRESSURE P MAX P MIN Flashing Display 50 0 0 100 0 FLOW PRESS PRESSURE P MAX P MIN Press the key 50 0 0 FLOW PRESS PRESSURE Flashing 29 Key in 3 0 and ENTER The display will change as illustrated below completing the setting Display 50 0 0 FLOW PRESS PRESSURE Example 3 Setting a lower limit pressure 20 kgf cm Display 50 0 0 FLOW PRESS PRESSURE Flashing Press the FUNC key two times Display 50 0 0 FLOW PRESS PRESSURE Flashing Key in 0 and The display will change as illustrated
7. Packing filter PREP Lock catch 228 17523 1 670 11610 01 1 Refer to Para 8 2 3 Refer to Para 7 5 Clamp 3136 03 Drain pipe assy 046 00994 03 2 228 20745 9 1 1 Weights of drain and cleaning pipe Connected to drain valve 3 Other Remarks Suction filter 228 17421 91 Refer to Para 3 3 Power supply cord 110 115 V 07 1 60816 01 or 1 Refer to Para 3 2 220 230 240 V 071 60814 02 Grounding adapter KPR 14 071 60803 01 Refer to Para 3 2 Japanese 228 10544 Instruction manual LC 8A Instruction manual of liquid transfer unit English 228 10545 Instruction manual Japanese 228 10546 i Instruction manual of overall system separation system English 228 10547 Caution When plumbing and carrying out maintenance be sure to use the parts described on this page or 10 1 Consumable Parts List and 10 2 Assembly Parts List Use of other parts may not assure normal function of the system 3 Installation 3 1 Installation Precautions Warning For correct and safe use of the system proper care about the installation site should be taken with paying attention to the following points 1 Ventilation Since the solvent used for the high performance liquid chromatograph is flammable and toxic be sure to ventilate the room 2 Fire Never use a fire in the place where the high performance liquid chromatograph is installed Also avoid to inst
8. 6 1 Power Supply Switch On When the power supply is switched on the contents of the memory in the unit are auto matically checked If the contents are normal the display on the front panel shows the version number for about 3 seconds and then the display shown in Fig 6 6 1 revealing that the unit is ready for operation Theindicated figures are different according to setting uz C FLOW C PRESS e AUX FUNC PROG SET FLOW PRESS PRESSURE P MAX P MIN Fig 6 6 1 Display at Power Supply Switch On a If some error has been detected in the internal memory one of the following messages is displayed and the audible alarm is signalled ROM FAILURE Abnormal in ROM RAM FAILURE Abnormal in RAM i If one of the above errors occurs it is not possible to continue the operation of the unit i Switch off the power supply and contact our branch office sales office or agent b TheRAM of the unit is a CMOS RAM The memory is protected by a lithium battery built in the unit against power supply interruption If the data stored in the memory are corrupt the message shown in Fig 6 6 2 is indicated on the display section NOT PROTECTED Fig 6 6 2 Display for Corrupt Memory 26 When the above display is shown the settings are all returned to the initial default set tings and the contents of the time program are all cleared Press the key and the audible alarm is silenced and operation can be continued Th
9. SCL 8A preparative system controller with the model PC 30L interface When several units are connected to the SCL 8A for setting up a high pressure binary or ternary gradient system one PC 30L interface is required for every LC 8A Installation of PC 30L Interface Board 1 Remove the power supply cord of the LC 8A Warning To prevent electric shock be sure to turn OFF the power switch and remove the power supply cord before removing the cover 2 Remove the upper cover of the LC 8A Unscrew the fastening screws and remove the rear panel Din Fig 7 1 3 Remove the screw in Fig 7 2 from the hexagon spacer in the unit 4 Fasten the PC 30L interface board to the rear of the unit with the screws removed in step 2 above Further secure one end of the PCB to the hexagon spacer with the screw removed in step 3 5 Connect the PCB connection flat cable in Fig 7 3 in the unit positively to the connector Ja of the PC 30L interface board 6 Disconnect the cord 2 in Fig 7 3 from the transformer cover at the upper part of the transformer and connect the cord to the connector JG of the PC 30L interface board 7 Replace the cover of the LC 8A and stick the seal supplied with the interface board to an adequate location on the rear panel Fig 7 1 48 Fig 7 2 Fig 7 3 49 8 Maintenance Maintain the LC 8A in good order by checking the following points from time
10. assy Fuse 10A 125V Fuse 7A 250 V Transformer assy ZNR ERZC10DK 431 Capacitor ECEGA2AY 153C Printed circuit board PC 1 assy CPU 80188 ROM ASSY RAM 4464 Lithium battery BR 2 3AE2N Switch SID 0601 Switch SID 0201 Crystal 16MHz HC18 U Printed circuit board PC 2 assy ROMI ASSY ROM2 ASSY IC AD7582KN Relay G2R 117P V 24V Fuse AC4 125 V Diode Ma651 Diode MA650 Transistor 2541261 Transistor 28C3157 Printed circuit board PC 6 assy IC AD625JN IC OP 07CN IC LFC4558C Part No 064 14534 33 228 18193 91 072 01028 072 01031 04 228 18194 92 06 1 80703 17 056 44333 12 228 17840 9 1 075 29338 01 228 18127 9 1 075 31296 02 074 73305 13 064 16201 06 064 16201 02 061 86406 228 17842 92 228 18129 91 228 18129 92 075 23677 01 065 61968 3 1 072 02001 18 075 39906 11 075 39906 01 670 14002 060 43 157 228 18071 9 1 075 23676 02 075 23652 02 075 31135 Remarks POWER switch Cooling fan For 100 120 VAC For 220 240 VAC Surge arrestor 15 0004F 100V capacitor Printed circuit board assy complete M101 ROMI M102 B102 Parts on PC 1 assy S101 x102 X101 Printed circuit board assembly complete ROM1 ROM2 M203 Parts on PC 2 assy RE1 F201 F205 D222 D223 D227 Q222 Q226 Q227 0231 Parts mounted on radiator on PB 2 assy Printed circuit board assembly complete A601 A602 Parts on PB 6 assy A603 69 S228 10545D A List of Errata for LC 8A User s Manual Before
11. follows 1 Tum the power switch CBF 2 Remove the power cord fram the power cord connector 3 With a slotted head screwdriver catch the cover on the fuse holder and slide it out Remove 4 After replacing the fuse push the fuse holder cover back in until it clicks 8 7 Cleaning If the cover or the front panel of the unit is dirtied wipe away the dirt or dust with a soft cloth or a piece of tissue paper If necessary use a synthetic detergent 63 9 Trouble Shooting Troubles arise from various causes and it is not possible to outline every trouble and cause This section outlines the locating methods of causes according to symptoms Contact our branch office sales office or agent if the cause of some troubles can not be located in the methods outlined in this section or spare parts for substituting faulty parts are not on hand If the LC 8A is connected to the SCL 8A system controller refer also to the instruction manual of the SCL 8A 9 1 Symptoms and Remedies of Troubles Liquid transfer is 1 Air in pump head 1 Discharge liquid from drain at flow rate 40 m2 min not stable for driving out air Pulsation of 2 Remove air from pump head with syringe connected pump is heavy through drain port Referto Para 6 3 2 Previous solvent left in 1 Raise flow rate and discharge liquid from drain port pump head for washing away previous solvent Refer to Para 6 4 3 Air in suction filter 1 Raiseflow rat
12. in Fig 6 5 Drive the distilled water into the cleaning flow line On completion of separation or analysis replace the mobile phase reservoir by a vessel filled with distilled or ion exchanged water for cleaning the injector column detector cell and others well before turning off the unit After that open the drain valve and drive distilled water into the cleaning flow line with the disposable syringe under a condition of transferring liquid at a flow rate of 10 m min Fig 6 5 Manual Cleaning Method Automatic Cleaning with FCV 130AL Read the instruction manual of the FCV 130AL or BL and install pipe the FCV 130AL Connect the mobile phase reservoir to RESERVOIR 0 and the column cleaning distilled water reservoir to RESERVOIR 1 On completion of separation or analysis switch over to the distilled water reservoir and clean the injector column and detector cell During operation run the rinse pump continuously for transferring liquid and recycling distilled water in the flow line Employ a large capacity reservoir for the rinse pump and exchange cleaning water at regular intervals Cleaning water should be exchanged frequently when a high concentration buffer is used as a mobile phase The FCV 130AL rinse pump and the switching over of the reservoir can be controlled by the time program from the LC 8A Refer to Para 6 7 Time Program for details 6 6 Setting for Constant Flow Rate or Constant Pressure Liquid Transfer 6
13. liquid 9 A B Electric charge which e e o9 o 57 cannot move being fixed 060000000000 to the solid surface 0000000090000000 VLE Solid Fig 1 Occurrence of static electricity by friction between solid and liquid When the charged liquid is collected in an insulated vessel i its static charge is gradually increased to a few kilovolts If someother conductive object is brought near the vessel Energy release by discharge electricity is discharged at a certain distance from the vessel releasing heat energy This heat energy iginites any near by combustible gas of Ignition of combustible substances If air is dry appropriate concentration Liquid is flowing at high speed through a small diameter tube Charge is casily accumulated if bubbles are included in the liquid Insulated vessel like polyethylene Combustible gas is stored in the head space of the vessel Combustible organic solvent is charged to a great extent Floor covered with rubber etc provides unwanted insulation Fig 2 Conditions for Accidents Preventive Measures against Accidents The principal preventive measure is on prevention of charging and storage of static electricity among those items shown in Occurrence mechanism of static electrical discharge accidents Although the preventive measures are shown below it is important to exercise two or more measures simultane ously Particu
14. the power supply Cautions About Unit Stop a Make sure that any buffer etc is completely flushed b If the drain valve is left open when the liquid level in the reservoir is high liquid may flow unintentionally siphon Close the drain valve when not trans ferring liquid o Drain valve left open o Mobile phase level in the reservoir high o Drain pipe outlet port low Caution In the above conditions liquid in the mobile phase reservoir may flow into the Fig 6 9 drain pipe Close the drain valve when it is not operated c When the power supply of the pump is switched off liquid may be unintentionally transfer red momentarily This however is not a fault d When the filter section of the suction filter is being exposed to air for along period of time put the filter in a vinyl bag or wrap it up with aluminum foil for protecting it from dust and dirt 6 10 Characteristic Check a Analysis Flow Rate Testing Connect 1 6 mm OD and 1 0 mm ID X 2m 1 6 mm OD and 0 1 mm ID X 8 m SUS316 tubes to the mobile phase outlet port of the pump Transfer water at 1m2 min set K REV to 0 85 record the pressure by the pen recroder and ascertain the following i Display value of pressure is 100 150kg cm ii Fluctruation of pressure is less than 10kg cm b Flow Rate Measurement Provide an adequate measuring pipet or cylinder Caution Since an error in measurement with the measuring pipet or cylinder i
15. to time and taking adequate actions for detected faults Para 8 1 outlines maintenance requirements for the overall preparative LC flow lines not limited to the LC 8A proper and Para 8 2 to 8 5 outline the maintenance requirements for the LC 8A 8 1 Inspection and Cleaning of Flow Line System for Clogging and Contamination 1 Inspection The flow line may be clogged or blemished if the pump discharge pressure is abnormally high the baseline of detector drifts or deflects extremely or the peak retention time or separability fluctuates In general clean the flow lines from upstream to downstream first of all 2 Cleaning a Cleaning of Line Between Suction Filter and Sample Injector If the suction filter is clogged with dirt in the course of time flow line resistance is increased causing for instance generation of bubbles disabling liquid transfer at an accurate flow rate If dirt deposits in the pump head check valve line filter etc not only is an accurate flow rate not acquired but also the baseline drifts as dirt enters in the column To clean the flow lines of dirt run for instance isopropyl alcohol as a mobile phase by the LC 8A b Detection Cell Cleaning Clean according to the instruction manual of each detector c Column Cleaning The column is probably fouled if the peak retention time or separability fluctuates or the baseline drifts though the composition and flow rate of mobile phase are unchanged If the column is foule
16. 00 0 T 0 01 FLOW PRESS PRESSURE P MAX P MIN Time is shown in minutes P MAX and P MIN are not shown The normal display how ever is shown when the time program is not running b MONIT REV 0 00 R FLOW PRESS PRESSURE The revolution number is indicated in terms of pump periods one period is transfer of liquid in one stroke from right and left heads each Revolutions are counted up to 16 777 215 and the count returns to zero PRESSURE P MAX and P MIN axe not displayed c MONIT PLNG 0 00 PLNG 6000 FLOW PRESS PRESSURE P MAX The plunger position is indicated in terms of steps of the stepper motor Oto 11 999 6 000 and 12 000 represent the extreme retraction positions of right and left plungers respectively PRESSURE P MAX and P MIN are not indicated d MONIT FLOW 10 00 FLOW 1 00 FLOW PRESS PRESSURE P MAX The approximate flow rate is indicated only in the constant pressure mode PRESSURE P MAX and P MIN are not indicated e MONIT COMP 0 00 COMP 5 00 FLOW PRESS PRESSURE P MAX PRESSURE P MAX and P MIN are not indicated Function Sets the compression factor of the liquid used for determining the flow rate from the compression factor The unit is 10 kgf cm y Generally employ the initial value 9 80 Setting Example 5 o ENTER Caution Don t carry out this function unless necessary as all the parameters and programs are cleared by this function Function
17. 00 kgf cm The unit can change parameters such as the flow rate automatically with time by the time program For instance liquid transfer can be stopped automatically or the liquid flow rate can The instruction set of the program is shown in Table 6 7 1 The FLOW instruction can change the flow rate linearly along the straight lines connecting the initial setting and each step setting Time is settable in a range between 0 01 minute and 999 99 minutes in steps of 0 01 minute Up to 32 steps can be programmed and the set program is preserved even if the power supply 0 1 m2 min 1 kgf cm One of three figures 18 valve option e Event output on off 1 2 3 Event 3 is on off of FCV 130AL cleaning Combinational pump Option Repetition of program from the top 0 255 Repetition number 0 is 256 times End of program N A r 6 7 2 Initiation of Time Program Mode 33 0 USED 32 LEFT Continue to press the FUNC key till the following display is shown The above display tells that the number of set steps is zero and the number of remaining programmable steps is 32 Press the ENTER key further and the following display is shown TIME FUNC VALUE nr 3 Flashing One step comprises time instruction and control value Flashing TIME means that control waits for the input of time Further press of the ENTER key shows a step as exemplified below 5 00 FLOW 50 0 When the key is pres
18. 228 10545A LC 8A PREPARATIVE LC PUMP UNIT USER S MANUAL READ AND UNDERSTAND THIS MANUAL BEFORE OPERATION SAVE THIS MANUAL SHIMADZU CORPORATION ANALYTICAL INSTRUMENTS DIVISION KYOTO VAPAN Copyright Shimadzu Corporation 1989 All rights are reserved including those to reproduce this publication or parts thereof in any form without permission in writing from Shimadzu Corporation Information in this publication is subject to change without notice and does not represent a commitment on the part of the vendor Any crrors or omissions which may have occurred in this publication despite the utmost care taken in its production will be corrected as soon as possible but not necessarily immediately upon detection Precautions for Safe Operation The LC 8A is the solvent delivery unit for the high performance liquid chromatograph In order to operate the unit for safety strictly observe the following points 1 Do not use the unit for the purpose other than the above mentioned analysis Follow the procedures described in the instruction manual Observe the warnings and cautions Do not disassemble or modify the unit without our approval For repairof the product inside contact our sales office or agent The pink color pages and the meshed parts are for our service engineers and not intended for our clients You are requested not to follow the instruction for installation It is dangerous F
19. 3 5 before operating the unit Remove two screws A and B fixing the cover Replace two screws with the pan Fig 3 5 Installation c Vat Cut the supplied silicon rubber tube to a necessary length Cut the tube slightly slant wise and the tube can be easily slipped into the hole Introduce one end of the tube into the hole in the left side face of the pan and put the other end in a waste liquid bottle Keep the end of the tube in the bottle away from the bottom Don t use the bottle for other waste liquids and empty the bottle from time to time for waste liquid can not be drained if the level of liquid in the bottle reaches the end of the rubber tube Fig 3 5 2 Caution Be sure to place the waste water bottle at the position lower than the system like on the floor and make plumbing of the drain tube downward After plumbing flow the water to the receiving pan to check that the waste liquid does not flow back and is introduced to the drain bottle 10 3 8 Connection of Drain Pipe Connect the supplied drain pipe to the unit as illustrated in Fig 3 6 1 Attach the supplied clamp as a weight to the end of the Teflon tube and introduce the tube into the waste liauid bottle Fig 3 6 2 Fig 3 6 1 Fig 3 6 2 Caution Don t return the waste liquid discharged from the drain pipe to the reservoir as the liquid includes particles produced by the wear of the pump seal If the liquid is re
20. 58 Inside dia Imm X 1 6 mm Pipe SUS316 right 228 22002 16 Inside dia 1 mm X 1 6 mm Pressure sensor ASSY 228 20275 91 Diaphragm strain gauge type Pipe SUS316 228 22001 40 Inside dia 1 mm X 1 6mm Drain valve PREP ASSY 228 17526 91 Including 15 Line filter Refer to Para 8 2 3 Duplex construction of 24 and 1u Drain pipe assy 228 20745 91 5 3 Left Side Face Fig 5 3 LeftSide of Unit S17 5 4 Rear Panel Fig 5 4 Rear Panel Power supply cord connector Input AC power supply terminal of unit couple supplied AC cord to this connector Fuse holder Housing 10A 125V fuse for 110 115V unit or TA 250V fuse for 220 230 240V unit Ground terminal Ground unit from this terminal when power supply outlet is 2 conductor type REMOTE connector Remote control connector Detailed application method is outlined in Para 5 5 PCV 130AL connector When optional model FCV 130AL BL is used connect the dedicated cord to this connector 2 and connectors are not existing in case of P N 228 18888 96 LC 8A 230V CE 18 5 5 Remote Connector Use The remote connector on the rear panel of the unit has the terminals shown in Fig 5 5 Use them according to the application purpose EVENT 1 EXT S EVENT2 PUMP ON OFF Close to turn pump ON lt Open to turn pump OFF PROG RUN same as PUMP on Close to RUN program front panel same as PROG RUN on front panel PRESSURE
21. Check the memories ROM and RAM Setting Press the ENTER key and the check of memories is carried out repeatedly The intention of this function is to check the characteristic of the memories in relation to temperature variation etc and the function is continued till the power supply is switched off If the memory is faulty the audible alarm is signalled and the message ROM FAILURE or RAM FAILURE is displayed HOME ADJ This function is used for adjusting the plunger home position The function however needs not be used because the adjustment was made before shipment 43 FLOW ADJ This function is used for adjusting the flow rate The function needs not be used because the adjustment was made before shipment LOCAL Function Though the flow rate can not normally be set locally by the LC 8A when the SCL 8A system controller is connected to the unit the setting by the LC 8A however can take priority by the LOCAL function When the SCL 8A is not connected the LOCAL function may be set or not Setting Key in L and for setting the flow rate by the LC 8A and 0 and for regulating by the SCL 8A When the flow rate is regulated by the SCL 8A the C FLOW light is lit indicating that operation is in the remote mode K COMP Function In the correction of pulsation the high speed travel range cam angle of the plunger is determined by the flow rate and pressure The angle however should be adjusted for s
22. G FLOW PRESS PRESSURE P MAX P MIN ENTER 0 USED 32 LEFT ENTER TIME FUNC VALUE Flashing 5 Input time for first step Keying In Display 15 00 FLOW Flashing 35 6 Input instruction Though FLOW flashes instructions are shown one after other by press ing the key successively Press the ENTER key when the concerned instruction is displayed and the instruction is then loaded KeyingIn ENTER Display 15 00 FLOW VALUE Flashing 7 Input control value KeyingIn 0 ENTER Display 15 00 FLOW 20 0 Flashing 8 Input for second step as in above steps Keyingin z C ENTER ENTER E lo Display 20 00 FLOW 50 0 9 Input for stop instruction step finally KeyingIn 3 0 ENTER FUNC FUNC FUNC FUNC ENTER Display 30 00 STOP 10 End of setting KeyingIn Display 3 USED 29 LEFT 11 KeyingIn Display 10 0 0 100 0 FLOW PRESS PRESSURE P MAX P MIN Flashing e When two or more steps are programmed input need not be made in the sequence of time The unit arranges the steps automatically e The stop instruction should normally be set at the end of the program except for a special case in which the program is run endlessly 36 Deletion of a Step Display a step to be deleted with TIME flashing and press the key 1 Display 5 00 FLOW 10 0 Flashing 2 Keying In Display 10 00 STOP Flashing In the above example the FLOW step is dele
23. all other devices that may spark in the same room Further an extinguisher should be installed for the accident 3 Sink A sink should be installed nearby for the case if the solvent entered eye or touched the toxic solvent 4 Corrosive gas and dust To assure long service life and good performance of the system avoid installation in a place exposed to corrosive gases or dust 5 Electromagnetic noise Avoid installation in the vicinity of such devices that generate a strong magnetic field Use an additional noise filter if the power line has much noise 6 Installation space requirements This system is designed to be used on a table or stand preferably a solid and flat surface with a depth of 80cm or more 7 Others In selecting the installation site take full care with regard to the following items in order to maintain the performance of the system 1 Room temperature should be within 10to 350C and without extreme fluctuations 2 Do not expose the system to the direct output from a heater or a cooler 3 Do not expose the system to the direct sunlight 4 The installation site should be vibration free 5 Relative humidity should be within 4596 to 8596 3 2 Electro Magnetic Compatibility Notice Description of this section are only applied to the model for EU European union market 228 18888 96 This instrument comply with European standard EN55011 class B for electro magnetic emission and EN50082 1 for electro mag
24. ction zone see A al Detailsof Parts i51 occu od eee a ada Installation usxor bx e RRERERETEARIRIAG eee eee eaten eee see PANDA Che see ieee NES Sob Wats tall gens TC CUL ODS ed d tate chad A A ates ater ea aid iia and Dusta ss oe o ake are momen wee 3 1 2 Ventilation and Open Flames iss oce cos A ROC C eae ee ee 3 153 Supporting Vablecc4 lt 444e tase A Ro EUR UNE Tra eet 3 1 4 Removal of Transportation Brace 42 42382 wa iR ee a Dee a Ro a ee 3 2 Electro Magnetic Compatibility 3 3 Removal of Transportation Brace 3 4 Connection of Power Supply 2 o A An 3 6 Preparation of Mobile Phase Reservoir seeren renere reen REE 3 7 Installation of Drip Pan III 3 8 Connection of Drain Pipe A deed A A AA 3 9 ORGS A Specifications a ec Re a a cnt moe a Us we ecce Componentsand FU ncllons iio CR ACE REOR AOL EE ELE E A 34 Pront Panel siren a A ace isle ta ee si ht S Lic eL dude EU ees J2 PL WALLS SMA RU au eh a rc cae ae RUE da S P Left Side PAGG most Ute bud od oul i hae aan eh anager uis eee lac a ade JA Rear Panels ed itis a isque ee eo eta EE Ue bedeutet ss 5 9 Remot Connector User id AAA REG P co dy Operation i es 6 Preparation or Mobil
25. d 10 or across terminals No 9 and 10 Pressure up to 100 kgf cm or 500 kgf cm can be recorded respectively 20 6 Operation 6 1 Preparation of Mobile Phase Cautions 1 Use clean mobile phase free from dirt and other contaminants 2 Low boiling point or high viscosity solvents are not suitable for liquid transfer n 3 Don t use solvents buffer mobile phase etc such as KCQ NaC and NH4C which include halogen ions or solvents producing halogen ions by reaction care should be exercised when a solution including a halogen is used regardless of their pH for they corrode the stainless steel flow line 6 2 Simple Operation Procedure for Constant Flow Rate Liquid Transfer Typical operation procedure for constant flow rate liquid transfer will be outlined in this section The detailed operation procedures for the keyboard are given in Para 6 6 1 Fill the reservoir with mobile phase and put the suction filter in it Follow the operation procedure outlined in Para 6 4 when the type of mobile phase is changed 2 Ascertain that the pipe is connected to the mobile phase outlet port 3 Make sure that the end of the drain pipe put into the waste liquid bottle Suction filter Display section Pump key Power supply Moble phase switch reservoir Drain valve Connected to injector Mobile phase outlet port Drain pipe Waste liquid bottle Fig 6 2 Preparation for Liquid Transfer
26. d is not flowing The motor may slip if the valve is closed abruptly when liquid flows at a high flow rate 12 Press the key for starting the pump Be sure that the pressure indicated is correct and liquid runs from the outlet of the fraction collector or the detector 6 3 Normalizing Method of Liquid Transfer Air Purging Method from Head The following irregularities occur if a considerable amount of air remains in the pump head 22 Irregularities in Liquid Transfer a Liquid is not transferred Liquid is not drawn up b Liquid is transferred but it flows and stops synchronized with the mechanical operating sound of the pump c The indicated pressure fluctuates remarkably synchronized with the mechanical operating sound of the pump at a low flow rate below 10 m min The above phenomena tend to occur after installation right after seal replacement after disassembly and cleaning of the check valves and when a considerable amount of air is taken in from the inlet port Normalize in the following steps Make sure that a high flow resistance part such as a column is connected to the carrier outlet port O Connect a disposable syringe to the drain pipe Fig 6 3 Run the pump at 40 m min and draw liquid into the syringe vigorously Fig 6 3 Connection of Disposable Syringe 6 4 Change of Mobile Phase Caution When the mobile phase is changed remove the column and comply with the f
27. d lightly run a clean mobile phase used for separation continuously for many hours at a pressure below the maximum operating pressure of the column and generally at an analysis flow rate or below If fouled heavily wash the column with a solvent adequate for the bed packed in the column For details consult the instruction manual of the column or contact our Analysis Center d Cleaning o Entire Flow Line System When a buffer or other liquid which produces crystals or residue when dried is used as a mobile phase don t leave it in the system over many hours Otherwise the pipe system may be clogged or the plunger seal may leak because of damage to the plunger or seal of the pump Each 50 time after such a mobile phase is used wash the flow line well with distilled or ion exchanged water It is recommended to comply with Para 6 5 If the buffer enters behind the pump head when a part such as the plunger is replaced and salt deposits on the bearing of the head holder or on the plunger remove each part and wipe off salt with gauze wet with water 8 2 Parts Replacement 8 2 1 Plunger Seal Replacement The service life of the plunger seal is dependent on the operating conditions It is roughly about 40 days if it is operated for instance 12 hours a day at a flow rate 150 m amp min and a pressure 80 kg cm with water as a mobile phase If the seal is aged liquid leaks from the seal and it is identified by liquid coming out of the cl
28. displayed when the display is shown Details of the auxiliary functions will follow Refer to Para 6 6 7 Change of Liquid Transfer Mode and Para 6 7 Time Program for MODE CHANGE and PROG SET 39 MODE CHANGE Function Changes the liquid transfer mode Setting Press the key and the mode is switched over between constant flow rate and constant pressure modes The mode however is not changed when the key is pressed while liquid is being transferred PROG SET Function Sets up the mode for setting the time program Setting Refer to Para 6 7 COND Function Self diagnoses pressure waveforms of transfer liquid and displays the results of self diagnosis Setting The display is shown without operator action The displaying messages and their meanings are shown in Table 6 8 2 Table 6 8 2 Messages on Results of Self Diagnosis Message Meaning Normal Left pump head pulsation high 20 or over Right pump head pulsation high 20 or over Both pump heads pulsation high 20 or over Pressure drops when left pump head discharges Pressure drops when right pump head discharges Pressure rises 10 or over in comparison with that of 5 minutes ago Pressure declines 10 or over in comparison with that of 5 minutes ago e X L R is displayed when the peak to peak pulsation pressure exceeds 20 the pressure setting e XX L R is displayed when liquid transfer from either of t
29. e liquid is released via vessel Fig 3 Anti Static Electricity Measures for Vessel Preventive measure 3 Do not approach the vessel with charged objects including the human body Charging prevention measures for human body a Prevention of charging of shoes and clothes b Grounding of human body c Make working floor conductive Suitable products to be used for those measures a b and c are available on the market When persons who use no charge prevention measures approach dangerous sections they have to be grounded beforehand For instance they should contact grounded metal by hand Preventive measure 4 Use pipes with inner diameter of 2 or more for waste liquid line for large flow rates Inclusion of bubbles in the tube may increase the amount of charging by ten times Check that there is no inclusion of air via tube joints Preventive measure 5 When it is impossible to make the vessel conductive exercise caution in the follow ing points a Set the vessel so that the pipe outlet will be placed below the liquid level in the vessel Or dip a grounded metal ex pipe connected to the main body of device in the liquid This method is not effective for liquid with small conductivity 10 9 s m or less b Use a vessel of the smallest possible capacity to minimize the damage by fire if it should occur c Prevent the room from being dry Humidity of 65 or more has charge prevention effects V Cautions Exerci
30. e CMOS RAM is not always faulty when the above warning is displayed The display may be indicated when a lightning surge enters or the battery is discharged below the service capacity c The following two messages may be displayed alternately when the power supply is switched on and operation can not be continued It means that the protection function is actuated for preventing very high pressure from being applied to the circuit as pressure exceeding 5 kef cm is detected when the power supply is turned on When the high pressure acting on the flow line is removed the above displays disappear and the unit can be operated 6 6 2 Functions of Display Section E 2 d AT wm CLOW 142011 WS AUXJUNC mus PROG SET FLOW PRESS PRESSURE P MAX PIN Amen ri PROG SET Fig 6 6 4 Display Section 27 Function Flow rate setting m2 min is shown in the constant flow rate mode and pressure setting kgf cm in the constant pressure liquid transfer mode Pressure kgf cm detected by pressure sensor is shown Upper pressure limit kgf cm is shown Lower pressure limit kgf cm is shown Constant flow rate Comes on in constant flow rate liquid transfer mode mode display light Constant pressure Comes on in constant pressure liquid transfer mode mode display light f AUX FUNC Comes on when an auxiliary function is selected display light PROG SET Comes on when editing program display light
31. e Phase td aaa AA aA 6 2 Simple Operation Procedure for Constant Flow Rate Liquid Transfer lesse 6 3 Normalizing Method of Liquid Transfer AirPurging Method from Head Rw Boa HR o DD 6 4 Change of Mobil Phase 23 6 5 Operation When Buffer is Used Application of Cleaning Flow Line 24 6 6 Setting for Constant Flow Rate or Constant Pressure Liquid Transfer 26 6 6 1 Power Supply Switch OH cee eee n hh hn 26 6 6 2 Functions of Display Section ccc cece eee eee eee eee 27 6 6 3 Functions of KEYS isses eee eee nnnm rn 28 6 6 4 Setting Proced re crore Ver ettet ra eos QA Vl dM Ok ut 29 6 6 5 Upper Pressure Limiter PMAX cece 3 6 6 6 ower Pressure Limiter P MIND ose ad oa 3 6 6 7 Change of Liquid Transfer Mode saa ix ami ROCA CIRCA ERROR LEICA La eae ia nears 32 6 6 8 Application of Constant Pressure Liquid Transfer Mode n n nnn 32 6 7 Time Program sse nnne 33 6 7 1 Functions of the Time Program sssen Hn nn nnn 33 6 7 2 Initiation of Time Program Mode nnne 33 6 7 3 Time Program Setting Steps s Henn nnn 35 6 7 4 Start and Stop of Time Program nn nn n nnn 37 6 3 LOOP Instruction ieee irene Peay Peel ee ee Sere 37 6 8 AUX FUNG rr ca aa ala D aiu d 38 6 8 1 Application of AUX FUNC eritir cee eens 38 6 9 Stop of Unit d Svat se v
32. e and discharge liquid from drain port for driving out previous solvent 2 Shake suction filter for drivingair away 3 If suction filter is clogged clean it in ultrasonic bath Replace it if ultrasonic cleaning is not effective 4 De aerate carrier 4 Poor check valve operation 1 Disassemble and clean check valve or replace it 5 Leak ofliquid from plunger Retighten head fastening bolts seal Replace plunger seal Replace plunger 6 Leakage from flow line Take adequate action 7 Flow line being clogged Clean or replace line filter Check flow line Pump runs but l Air trapped in head Run liquid at 40 m min from drain port for driv liquid does not ing out air Refer to Para 6 3 flow Remove air by syringe through drain pipe 2 Airis drawn from joint Connect positively between suction filter arid inlet pipe recycle 3 Broken suction filter or 1 Replace faulty part inlet pipe Faulty joint 64 4 Wrong installation of check 1 Inspect and correct Para 8 5 valves or poor valve part assembly 5 Poor installation of seal or 1 Inspect Para 8 2 plunger Pump generates 6 Motor slippage 1 Turn pump off and on sound but does not run lower than 2 Suction filter clogged 2 Clean or replace suction filter setting Pump generates 1 Pump head is assembled Reassemble correctly abnormal noise cocked Click synchro 2 Poor lubrication Lubricate Para 8 4 nized wi
33. e can be disassembled cleaned and reassembled like the outlet check valve 1 Remove the check valve with the supplied adjustable wrench The valve is tightened with a considerably strong force Exercise care or parts may drop when the valve is turned upside down 2 Disassemble the valve on gauze spread on a table Take out the parts by pushing them with a forceps etc from the other side through the hole in the cartridge 3 Check the ball contact face of the ball seat D for chipping and dirt like with the outlet 60 check valve o Replace the ball and ball seat as a pair if chipped o Wipe off with gauze wet with solvent if fouled o Clean ball if fouled Exercise care not to lose it ZZA WN 77 E 2 po c x om A PSN LA LD n an rae AP G 7 7 S Plastic packing A Ball housing Ball Plastic packing C Seat housing Plastic packing D Ball seat Plastic packing A Cartridge Inlet check valve assy complete Fig 8 5 2 1 Part Number 228 17776 01 228 17782 01 018 98002 0 1 228 17776 03 228 17774 01 228 17776 04 018 98002 01 228 17776 01 228 17530 01 228 20256 9 1 Inlet Check Valve 61 Ground face 4 On completion of inspection of the ball seat and ball clean all parts in isopropyl alcohol or other solvent in an ultrasonic cleaning bath The ball and ball seat must be in a pair Assemble the check valve paying atte
34. eaning pipe If the FCV 130AL rinse pump is connected check for liquid by disconnecting the Teflon pipe If liquid leaks retighten the four screws of the pump head with the supplied allen wrench at first When further retightening is not possible or liquid leaks still after retightening replace the plunger seals according to the illustrations below Two spare plunger seals UR304 are supplied as standard spare parts If all the spares are used contact our service engineer for procuring other spares i E E x pep Disconnect the pipes from O Loosen the four 3 Pull out the head with the check valves at upper head fastening bolts care towards the operator and lower parts of the pump alternately a little at head a time and remove them Illustration shows removed upper and lower check valves Remove them how ever only when internal parts of check valves are be ing cleaned or replaced 51 4 Remove the seal with a tool 5 Clean the head inside free 6 Install the head straight like forceps with pointed ends of dirt Put a new sealin Exercise care not to scratch with the thumb straight the inside of the head with care not to cock the seal D Pressing the center of the Reconnect the pipes to head firmly with the left the upper and lower hand thumb tighten the check valves of the head head fastening screws Tighten the four bolts alternately a little at a time and final
35. erminal on the rear panel of the unit Warning To prevent electric shock and to secure safe operation of the system always make grounding 3 Making sure that the power supply switch located on the left side of the unit is in the OFF position connect the power supply cord to the receptacle on the rear panel of the unit Refer to the instruction manual of the system controller when the unit is connected to the model SCL 8A system controller 3 5 Piping 1 Connection of Suction Filter Connect the supplied suction filter to the inlet of the pump as illustrated in Fig 3 3 1 Tighten the filter firmly by turning the plastic grip of the filter with the fingers es Fig 3 3 1 Connection of Suction Filter 2 Connection of Pipe to Carrier Outlet Port Cut the supplied pipe SUS316 TP 1 6 X 0 8 to an adequate length and connect it to the mobile pahse outlet port of the pump Mobile phase outlet port Pipe Ferryle Male nut SUS 316TP Fig 3 3 2 Connection to Mobile Phase Outlet Port Determine the length of the pipe according to the system instruction manual when the pipe is being connected to an injector or a column switching valve Comply with the following cautions when the pipe is connected Note 1 Cut the pipe perpendicular to the pipe axis and finish the cut face flat and smooth 2 Thejoint is available in two types new and old The model LC 8A system employs the new type joints except f
36. ge Mar CS Limiter Threshold Pressure 0 50 m min 300 kgf cm 320 kgf cm 50 1 7 100mZ min 180 kgf cm 200 kgf cm 100 1 150m min 80 kgf cm 100 kgf cm The above maximum operating pressures include some allowances for eliminating the effect o pressure noises 6 6 6 Lower Pressure Limiter P MIN Liquid transfer is automatically stopped if the pump delivery pressure does not rise to the given P MIN setting in one minute after liquid transfer is started Whereby damage is minimized even if the flow line is leaking When the limiter is actuated the audible alarm is signalled and the following message is ERROR P MIN To disable the lower pressure limiter set the P MIN value to zero displayed 31 6 6 7 Change of Liquid Transfer Mode Switching over between the constant flow rate liquid transfer mode and the constant pressure liquid transfer mode is made as stated below Press the FUNC key till the following display is shown 0 00 MODE CHANGE FLOW PRESS PRESSURE P MAX P MIN The FLOW PRESS display always shows a flow rate or pressure setting The liquid transfer mode is changed each time the ENTER key is depressed The C FLOW light is lit in the constant flow rate mode and the C PRESS light is lit in the constant pressure e M mode indicating the selected mode by the ENTER key Press the key after changing the mode and the original display is show
37. gged with worn particles of the seals etc in the course of time The line filter should be cleaned or replaced if pressure rises when liquid is transferred though a flow resistance such as the column is not connected to the mobile phase outlet port of the pump a Line Filter Cleaning Method D Remove the line filter 2 Clean the line filter with with the supplied isopropyl alcohol in an wrench ultrasonic cleaning bath 54 3 Attach the line filter to 4 Run liquid through the line the drain valve and connect filter mobile phase outlet the pipe as before port and ascertain that pressure does not rise Fig 8 2 3 1 Line Filter Cleaning Method The filter should be replaced if flow is not improved by cleaning or the cleaned filter becomes clogged again in a short time Besure all solvents are analytical grade or better b Filter Replacement 2 Filter One 2 filter and one packing filter are supplied as spares D Remove the line filter 2 Grip the filter with 6 Put in the new filter and with the supplied wrench pliers and take it out packing It can be taken out with pliers and an adjustable wrench Note Replace the 14 filter only when flow is not improved by replacing the 2 u line filter 4 Introduce the line filter into the drain valve and tighten the filter firmly with the wrench Reinstall the pipe Fig 8 2 3 2 Line Filter Replacement 3 55 Element as
38. he cover Do not remove the cover at the normal maintenance Contact our sales office or agent of repair requires to remove the cover of the main body Precautionson Static Electricity while Using Liquid Chromatograph Liquid chromatography using combustible organic solvents as mobile phase requires constant exercise against fire explosion etc Particularly among various possible accidents those caused by static elec tricity are difficult to anticipate and tend to occur only with unexpected conditions which often make countermeasures insufficient At a site where preparative liquid chromatograph is practised a large amount of combustible substances may be used Therefore once an accident happens it could lead to tremendous damage The mechanism of accident by static electrical discharge and preventive measures are described below Exercise due care in safety measures in handling of equipment An abstract of the following description is expected to be included in the instruction manual of the liquid chromatograph and related equipment Mechanism of Static Electrical Discharge Accident Example Accidents caused by static electricity take place through the following processes When liquid is fed at high speed through a small diameter tube like the pipe of a liquid chromatograph static electrical charge occurs by mass flow charge displacement as shown in Fig 1 A Electric charge moving Flowing liquid with flowing
39. home position deviates In this case the pump is stopped 66 If the motor slips when liquid is transferred at a considerably high flow rate the message may be displayed again when the pump is started up again In this case press the CE key and start up by pressing the key The pump will locate the home position at a low speed and start liquid transfer at the given flow rate A conceivable cause of motor slippage is abrupt loading of the pump 67 10 Parts List 10 1 Seal UR304 Seal 2004 Lubricating oil Content 5 m2 Line fdter Assy Suction filter PREP ASSY Plunger assy 10 2 Assembly Parts List Z F VD ON Un A LU Nea Mechanical Flow Line Parts Head holder Wiper Drain valve assy O ring Teflon Packing drain Motor Small pulley Belt Photosensor assy 228 18092 228 18306 228 09408 92 228 18405 91 228 17421 91 228 16837 91 228 16833 228 20684 228 17526 91 036 11402 228 17489 02 228 16816 228 16793 032 2 1975 49 228 20390 91 Plunger seal Spares are supplied Cleaning flow line seal Spares are supplied Spares are supplied 2 u line filter Spares are supplied Remarks Silicone rubber plate installed behind head holder Included in drain valve assy n Refer to Para 5 2 for other flow line parts Para 8 2 3 for the line filter and Para 8 5 for the check valves Electrical System Part SW HLS208K12 G Fan
40. ing and subsequent mobile phases Substitute the preceding mobile phase with the transitional cleaning liquid and then the transitional cleaning liquid by the succeeding mobile phase Each sub ome stitution should comply with the steps Dto 5 in the preceding paragraph Substitute the preceding mobile phase also in the detector flow line with isopropyl alcohol once Refer to the instruction manual of the detector for the cleaning method 6 4 3 Change of Buffer cee To change from a mobile phase such as a buffer producing crystals or residue clean the flow f line well with distilled water or water purified by ion exchange and fill with new mobile phase Crystals or residue may become unremovable if an organic solvent such as isopropyl alcohol is run before running distilled or ion exchanged water 6 5 Operation When Buffer is Used Application of Cleaning Flow Line When a buffer is used the back side of the plunger seal and the plunger surface should be washed with distilled water as the plunger seal and plunger are damaged by the deposit of the solute of the buffer Cleaning may be made automatically by the FCV 130AL available sepa rately or manually with the supplied disposable syringe The use of the FCV 130AL is recom mended when alow concentration buffer is used intermittently 24 a b Manual Cleaning In manual cleaning draw up distilled water into the disposable syringe and connect the syringe as illustrated
41. larly when a large quantity of combustible solvent is held in a large vessel be sure to observe the preventive measures 1 2 and 3 Preventive measure 1 Use metallic conductive waste liquid vessel which is well grounded This releases the charge of the waste liquid and vessel to ground The following items are available 1 Vessel equipped with grounding cord and clip P N 228 21353 91 2 Metallic 182 can P N 038 00044 3 Metallic 4 can P N 038 00043 Be sure to ground the vessel properly Disconnecting of grounding cord or poor grounding defeat the purpose of using a metallic vessel There are some metallic cans which have no conductivity due to an oxidized coating or lacquer on their surface Be sure to check that vessels are gorunded by a tester before application When a liquid with almost no conductivity of 107 s m or less is discharged into the vessel it is necessary to mix it with another liquid with some conductivity The other liquid can be placed in the vessel in advance Preventive measure 2 Minimize the clearance of both inlet and outlet of vessel to prevent an ignition source from entering the vessel 4 Vessel provided with three holes for 3 caps for 18 and 4 cans P N 228 21354 Connect clip to metallic part Connect to the grounding etc of the device to pound the metallic vessel Minimize clearance by attaching a cap Metallic 182 can Coated can is recommended Static electricity of th
42. ly firmly to avoid cocking the head Fig 8 2 1 Replacement of Plunger Seal ED 8 2 2 Cleaning Flow Line Seal Replacement The cleaning flow line seal needs not be replaced when cleaning liquid is not pumped If liquid leaks from the hole below the head holder and along the groove of the panel when cleaning liquid is passed through the cleaning flow line the cleaning flow line seal should be replaced Two spare cleaning flow line seals 2004 are supplied as standard spare parts Replacing Steps for Cleaning Flow Line Seal The right and left heads are of identical construction Disconnect the Teflon pipe from the cleaning flow line 2 Remove the pump head according to D to of Fig 8 2 1 A leak from the flow line seal B leak from the plunger seal Fig 8 2 2 1 Check of Cleaning Flow Line Seal for Leakage 3 Hold the upper and lower 4 Remove the seal from 5 Push the new seal into the pipes with the fingers and the case by pushing it case with the fingers with remove the seal case with forceps the visible spring side of Cleanthe seal case the seal first if fouled 53 6 Return the seal case in D Install the pump head Connect the Teflon pipe place with the visible as in Fig 8 2 1 to to the cleaning flow line seal side inward Fig 8 2 2 2 Replacement of Cleaning Flow Line Seal 8 2 3 Line Filter Cleaning and Replacement The line filter of the pump is clo
43. n Caution The mode can not be changed while the pump is transferring liquid Turn off liquid transfer before changing the mode in the steps outlined above 6 6 8 Application of Constant Pressure Liquid Transfer Mode Although the parameter in the constant flow rate liquid transfer mode is a flow rate m min that in the constant pressure mode is pressure kgf cm Table 6 6 3 shows the pressure setting range Table 6 6 3 Liquid Transfer Pressure Setting Range Parameter Setting Range Min Unit Initial Setting PRESS 10 300 kgf cm 1 kgf cm 10 kgf cm Actual maximum flow rates however are limited as shown in Table 6 6 4 according to the pressure settings in the constant pressure mode Accordingly the actual pressure may be lower than the setting under some loading condition Table 6 6 4 Maximum Flow Rates in Constant Pressure Mode Pressure Setting Maximum Flow Rate 10 100 kgf cm Approx 150 m amp min 100 200 kgf cm Approx 100 m min 200 300 kgf cm Approx 50 m2 min 32 6 7 Time Program 6 7 1 Functions of the Time Program be changed automatically in a given time 0 6 sec is switched off Instruction Description mode Pressure workable only in constant pressure mode Opening and closing FCV 130AL solenoid Flow rate workable only in constant flow rate Table 6 7 1 Instructions Usable for Time Program Setting Range 0 150m2 min 10 3
44. netic interference 1 Electro Magnetic Emission This instrument comply with European EMI Electro Magnetic Interference standard EN55011 class B equipment The standard classifies that class B equipment are suitable for use even in domestic environment 2 Immunity to Electro Magnetic Interference This instrument comply with European standard EN50082 1 for electro magnetic immunity Stated below are the test conditions 1 Immunity to Electro Static Discharge TEC801 2 8kv Air Discharge 2 Immunity to Strong Electro Magnetic Field IEC801 3 3v m Performance criteria for the test was as follows Display on the operation panel should not be changed except for actual pressure read out value Actual pressure shift caused by 3v m Electro magnetic field should not exceed 20 kgf cm 3 Immunity to burst fast transient noise from power supply IEC801 4 2kv to AC power line and protective earth Notice Compliance to the standard does not ensure that the instrument can work with any level of Electro Magnetic interference stronger than the level tested Interference greater than the value specified in the condition above may cause malfunction of the instrument To avoid electro magnetic distrurbance following notice are recommended to keep 1 Before touching the instrument Discharge the electro statics charged in your body to ground by touching metallic structure connected to ground 2 Do not install this instrument in such environment where
45. ntion to the orientation of the ball seat Assemble so that the ball comes into contact with the ball contact face of the Ball contact face thick line ball seat Smooth surface Joints between parts are sealed with packings KO 6 and 8 Slightly rough surface Be sure that they are installed correctly Packing A e stretches as if to embrace Fig 8 5 2 2 Ball Seat ball housing 2 as shown in Fig 8 6 2 3 in the course of time This however is not a fault Packing D 6 obstructs motion of the ball if stretched excessively 25 In this case replace packing D Replace packings if irregularities such Fig 8 5 2 3 Stretch c Packing A as cracks or deformation are detected Assemble the inlet check valve to the pump head Tighten firmly with the supplied adjustable wrench otherwise a gap is produced around packings causing leakage and other defects Caution The inlet check valve must be tightened firmly unlike outlet check valve Don t tighten the outlet check valve with the adjustable wrench This will exert too much force 62 8 6 Replacement of fuses warning This unit uses the following fuse Be sure to replace the fuse of same type and capacity Rated voltage 100 115 V Pat No 072 01028 10AF 125V 6x 30 Rated voltage 220 230 2409 V Part No 072 01031 04 7AF 250V 6x 30 Remove the power cord from the power cord connector When the fuse is blown replace it as
46. oie afraid dd 45 6 10 Characteristie Check iiss VA PN a dass itis ware aaa e tete Ou Du Siecle AS 46 PC 30L Interface for SCL 8A Use 48 MAlten anciana cies 50 8 1 Inspection and Cleaning of Flow Line System for Clogging or Contamination 50 8 27 Parts Replacement aia a rt LA a ceca ad 51 8 2 1 Plunger Seal Replacement iaa a uer a da eae 51 8 2 2 Cleaning Flow Line Seal R placementionia nilo da ls 53 8 2 3 Line Filter Cleaning and Replacement nnn nnn 54 8 2 4 Plunger Replacement ee n n n 57 8 3 Zero Point Adjustment of Pressure Sensor Scion 58 SA Lubricator Sud eres a id qs 58 8 57 Inspec omot Che ENNS ar o sS senis 8 5 1 Outlet Check Valve Inspection 8 5 2 Inlet Check Valve InspeolODo aa du ao dd ERR RR aaa ee DR idad 8 6 Replacement of fuses 8 7 Cleaning 9 Trouble Shooting 9 1 Symptoms and Remedies of Troubles sa vedi a wires E ali s E na ee dae es 9 2 Error Messages 10 Parts List 10 1 Consumable Parts 15 ee hh RR ee eee eee ees 10 2 Assembly Parts List 59 59 60 63 63 64 64 66 68 68 68 1 Introduction The model LC 8A liquid transfer unit is a pump designed for a liquid chromatograph separating and is
47. olating sample components in small to large quantities The discharge pressure is as high as 300 kg cm for high resolution separation and isolation and the range of flow rate is as wide as 0 1 to 150 m min for high efficiency separation The unit is also provided with a pulsation correc tion function and a flow rate correction function for fluid compression ratios in order to transfer liquid stably and highly accurately The unit offers high performance not only at large flow rates but also at small flow rates as low as around 1 m amp min for analysis permitting use of the unit as a liquid transfer pump for an analytical column The flow rate and other parameters can be auto matically changed by a time programming function The unit also provides the following functions in conjunction with other units Excluding P N 228 18888 96 LC 8A 230V CE o High pressure gradient function for separating complicated composition samples in conjunction with the model SCL 8A and others o Recycle function for separating components of unresolved peaks in conjunction with the recycle valve o Pump feed function for feeding a great amount of sample repeatedly in conjunction with an FCV 110AL and FCV 130BL o Pump seal cleaning function when a buffer is used in the mobile phase in conjunction with an FCV 130AL Thus a variety of user requirements can be satisfied This instruction manual provides information and instructions only on the LC 8A Refer to the ins
48. ollowing steps except when using the column continuously If a liquid not appropriate to the packed bed enters the column may deteriorate Comply with the instructions stated on the instruction sheet for the column 23 6 4 1 Change to Mobile Phase Miscible with Prior Mobile Phase 1 Put about 200 m of the mobile phase to be used next in a separate clean 500 m beaker 2 Remove the suction fileter of the liquid transfer pump from the preceding mobile phase bottle Dip the filter in the mobile phase prepared as in the above step 1 and shake the filter slightly to mix the liquids well 3 Open the drain valve for discharging the preceding mobile phase thoroughly from the flow line at a flow rate of about 40 m2 min 4 Flush the preceding mobile phase out for about 4 minutes at a flow rate of 40 m min About 160 mi of carrier is necessary for thoroughly washing out about 15 mi of the preceding mobile phase from between the suction filter and the drain outlet port 5 Dip the suction filter in a separate bottle of the new mobile phase Lower the flow rate a little and close the drain valve Clean the inside of the injector 6 F inally connect the column and detector and clean the entire analysis flow line The constant pressure liquid transfer mode is effective for cleaning 6 4 2 Change to Mobile Phase Immiscible with the Prior One Provide a transitional cleaning liquid such as isopropanol miscible with both preced
49. ome solvent The K COMP function is for setting the correction factor for the above purpose Actual correction angle K COMP X standard correction angle Pulsation correction is not made for a flow rate higher than 30 m min and the K COMP value is not valid Setting Example 1 C 1 15 Setting Range 0 to 3 in steps of 0 01 Rough guide for correction values is stated below Highly accurate correc tion can be made by adjusting according to pressure variation indicated by the recorder Methanol 1 00 Water 0 85 Hexane 1 15 K REV Function Sets a correction factor for increasing or decreasing the revolutions of the cam further in proportion to the pressure the revolutions are determined by the flow rate pressure and compression ratio Actual revolutions 1 K X Press X Typical revolutions where K K REV 0 1 kgf cm K REV 0 2 gt 0 1 per 1kgf cm 44 K REV 0 1 gt 0 per 1kgf cm K REV 0 gt 0 1 per lkgf cm Setting Example 0 0 8 ENTER 0 88 Setting range 0 to 0 2 in steps of 0 001 CLOSE KEY Function Disables input by al the keys Setting Press the key The FLOW PRESS flashing display is shown but key in by all the keys is disabled after that The message KEY CLOSED is displayed every four strokes of keying in Press the key and key at the same time for enabling input by the keys 6 9 Stop of Unit Observing the following cautions stop the pump and switch off
50. or fouled with dirt Replace with a new ball seat if chip Ball contact face thick line ped and wipe off dirt if fouled Wipe the ball and housing carefully with ethyl alcohol etc 5 On completion of inspection of the ball seat Fig 8 6 1 2 Ball Seat assemble the check valve paying attention to the orientation of the ball seat Assemble so that the ball comes into contact with the contact face of the ball seat Assemble with care referring to Fig 8 6 1 1 since orientation of the housing is different for outlet and inlet check valves 6 Joints of the parts in the check valve are sealed with plastic packing A seat A and packing B Be sure that they are installed correctly Typical faulty conditions are excessive stretch of seat A causing cocking of the ball seat disturbing make and break of the ball and ball seat and swelling of packing B making it difficult to put it in the cartridge In such a case replace with a new seat A and packing B 7 Assemble the check valve to the pump head Don t tighten more than necessary otherwise packing B may be damaged Note Check valve repair parts kit P N 228 11200 91 includes ball ball seat packing A packing B and seat A two each 8 5 2 Inlet Check Valve Inspection The arrangement of the inlet check valve is shown in Fig 8 5 2 1 The construction is differ ent from that of the outlet check valve and the size of the ball seat is also different from that of the outlet check valve The valv
51. or maintaining the unit in good order over many years Relubricate every 2 years Remove the cover of the LC 8A and that of the pump Running the pump feed about 10 drops of ail to the two points each shown in Fig 8 5 Use the supplied lubricating al and not any other Warning To prevent electric shock be sure to turn OFF the power switch and remove the power supply cord before removing the cover Lubricating point Fig 8 5 Lubrication 58 8 5 Inspection of Check Valve 8 5 1 Inspection of Outlet Check Valve Ground face Packing A 228 09028 Housing 228 09027 01 Pall 018 98002 SeatA 228 0957 4 Ball seat 018 98002 Packing B 228 09029 Cartridge 228 15637 01 Outlet check valve assy 228 09054 93 complete Fig 8 5 1 1 Outlet Check Valve The arrangement of the outlet check valve is shown in Fig 8 5 1 1 The valve can be easily disassembled cleaned and reassembled by the user The methods will be outlined 1 Remove the check valve from the pump head Use the supplied 13X17 wrench 2 Disassemble the check valve in the steps stated below Hold the portion a with pliers and remove Packing B 6 from cartridge 2 i 3 Tap cartridge O to remove the housing 2 Housing 2 seat A 4 and ball 3 59 may be scattered when disassembled Exercise care 4 Check ball O contact face of ball seat S refer to Fig 8 6 1 2 carefully with a 10X magnifier to see if the face is chipped
52. or safety operation this unit is provided with the AN mark at the portion where special cautions are required When operating the parts where this mark is indicated exercise special caution after reading the manual Warnings in the instruction manual This instruction stipulates the content of warnings as follows WARNING CAUTION NOTE Applied for the case that may result in death or serious injury Applied for the cases that may result in slight injury or physical damage Applied for improvement of operating efficiency or help for understandingthe article This unit is provided with the A mark at the portion mentioned below Before replacing the fuses be sure to tum OFF the power switch and disconnected the power cable from the power supply connector When operating the parts where this mark is indicated exercise special caution after reading the manual Warn ing e Check that the power switch of the main unit is set to OFF e This unit uses the following fuse Be sure to replace the fuse of same type and capacity Rated voltage 100 115 V Pat No 072 01028 10AF 125V 6x30 Rated voltage 220 230 2409 V T Part No 072 01031 04 7AF 250V 6x30 x BAS Ming label AN N Precaution on installation site and handling of the unit High performance liquid chromatograph which often requires large volumes of organic solvent requires proper care in handling and an appropria
53. or the joints of some column 1 Connection with Nut 1 6N or Female Nut 1 6FN Cylindrical portion New type joint Female nut Ferrule iy 1 6FN 1 6F 7 uu ld t joint peon Male type connection port common to new and old type Nut Back ferrule Front ferrule The male type connection ports are not much different and can be connected with either new or old typejoint 2 Connection with Bushing 1 6B or Male Nut 1 6MN Cylindrical portion New type joint a pe t Ferrule i is SN 1 6F Female S connection port new type Old typejoint HANA Be Front ferrule NU Female type connection 1 6FF Back s port old type Connect the bushing 1 6B or male nut 1 6MN to the corresponding female connection port The new type male nut and the female nut are easily identified with an about l mm height cylindrical portion adjoining the wrench application flats 3 Bottom the pipe in the opposite joint and fasten the pipe with the nut or the bushing 3 6 Preparation of Mobile Phase Reservoir The inside diameter of the opening of the reservoir must be at least 26 mm for receiving the suction filter The reservoir should be located within 1 m below the pump The location must not be higher than the pump Fig 3 4 NO YES NO Fig 3 4 Level of Reservoir 9 3 7 Installation of Drip Pan The drip pan is supplied for safely draining liquid which may leak from the LC 8A Install the pan as illustrated in Fig
54. ow rate of 5 0 the flow rate is changed as shown in Fig 6 7 3 37 TIME FUNC VALUE 35 00 FLOW 5 0 35 01 FLOW 10 0 40 00 LOOP 3 0 ml min 10 5 0 40 80 120 min Fig 6 7 3 Change of Flow Rate The steps of the program from the top to the LOOP instruction step are repeated a specified number of times The parameters such as the flow rate are returned to the initial values settings before the execution of the program before starting the next cycle Notes e Steps set after the LOOP instruction are ignored e Repeat times can be set up to 255 If 0 is set however the program is repeated 256 times 6 8 AUX FUNC 6 8 1 Application of AUX FUNC When normal operation is carried out the display shown in Fig 6 8 1 is indicated If the key is further depressed in the P MIN input mode the display shown in Fig 6 8 2 is indicated After that if the key is further pressed successively the displays shown in Table 6 8 1 are indicated one after the other A desired function of those shown in Table 6 8 1 can be performed by adequate manipulation when the function is displayed The functions in Table 6 8 1 are called the auxiliary functions AUX FUNC 0 00 0 100 0 FLOW PRESS PRESSURE P MAX P MIN Fig 6 8 1 Normal Panel Display 0 00 MODE CHANGE Fig 6 8 2 Example of AUX FUNC Display 38 Table 6 8 1 List of AUX FUNC Display Name Function MODE CHANGE Used for changing liquid transfer mode
55. rain valve Wetted part materials Suction filter Line filter Operating temperature Dimensions Weight SUS316 Hastelloy C ruby sapphire KelF reinforced Teflon Vespel 10 um land 2 um duplex construction 10 C to 35 C Width 350 mm depth 450 mm height 210 mm excepting projections Approx 32 kg Power supply LC 8A is available in the following versions according to power supply voltage ratings P N 228 18888 91 90 to 110V 7 5A 50 60Hz P N 228 18888 92 105 0 125V 7 5A 50 60Hz 12 P N 228 18888 93 P N 228 18888 94 P N 228 18888 95 P N 228 18888 96 200 to 240V 220 to 2605V 210 to 250V 230V 3 54 3 54 3 5A 3 54 13 50 60Hz 50 60Hz 50 60Hz 50 60Hz 5 Components and Functions 5 1 Front Panel O 6 Fig 5 1 Front Panel No Name Description Function 1 Keyboard 2 Outlet check valve i Outlet side check valve 3 Inlet check valve Inlet side check valve Pump head Plungers reciprocate inside for transferring liquid Head holder Supports plungers Incorporates seal cleaning line inside za Cleaning line inlet port Piping method is outlined in Para 7 5 Pump inlet port carrier inlet port Suction filter is to be connected to this inlet port 9 Zero point adjuster of pressure sensor Adjusting method Remove the white cap Making sure that the pump is s 1to 0 4 Drain valve U
56. ram By connecting the contacts to the input terminals of some other analyzer or another LC 8A the analyzer or the LC 8A can be started at the same time as the unit by pressing the PROG RUN key of the unit The contacts are closed for about 0 6 sec for starting Setting Key in and ENTER for setting this function and key in 0 and ENTER for resetting this function Note When the EXT S function is selected selection of EVENT 1 function outlined in the preceding paragraph is ignored EVENT 1 set in the time program is not executed 41 Function Displays the integrated number of pump revolutions Function Displays the position of the plunger MONIT FLOW Function Displays the flow rate in the constant pressure mode LJ MONIT COMP Function Displays a value corresponding to the compression ratio used for flow rate correction Setting Key in and for setting each function Key in 0 and for resetting each function If any one function is set other functions are automatically reset except for MONIT TIME The MONIT TIME function is not workable when some other Function Displays time in time program run function is set Examples of MONIT function displays will be shown below The displays are shown only when control waits for FLOW PRESS input Normal displays are shown when the P MAX or P MIN input waiting condition is set up by pressing the FUNC key a MONIT TIME 0
57. reading the manual please correct the items listed below Previous Revised Page II Warning labels indicated Rated voltage 220 230 Rated voltage 220 230 on the equipment 240V 240V Part No 072 01031 02 SAF Part No 072 01664 33 SAT 6 Replacement of fuses Rated voltage 220 230 Rated voltage 220 230 240V 240V Part No 072 01031 02 SAF Part No 072 01664 33 SAT Warning Rated voltage 220 230 Rated voltage 220 230 240V 240V Part No 072 01031 02 5AF Part No 072 01664 33 5AT Electrical System Fuse 5A 250V Fuse 5AT 250V No 3 Part No 072 0 1031 02 Part No 072 01664 33
58. s 1 to several percent the following measurement includes some error i Measurement of 1m2 min Flow Rate Connect SUS316 tubes of 1 6 mm OD and 1 0 mm ID X 2 m 1 6 mm OD and 0 1 mm ID X 2 m to the mobile phase outlet port Run water at 1 m min and measure the time required for pumping 2 m water The flow rate calculated from the measured time should satisfy 1 0 02 m2 min i Measurement of 5 m min Flow Rate Measure as in the above para 1 Resistance tube 1 6 mm OD and 1 0 mm ID X 2 m 1 6 mm OD and 0 1 mm ID X 2m Required flow rate 5 0 1 m amp min Amount of water to be measured 10 m iii Measurement of 20 m min Flow Rate Measure as in the above para i Resistance tube 1 6 mm OD and 1 0 mm ID X 2 m 1 6 mm OD and 0 3 mm ID X2m Required flow rate 20 0 4 m min Amount of water to be measured 40 m2 iv Measurement of 50 m min Flow Rate Measure as in the above para i Resistance tube 1 6 mm OD and 1 0 mm ID X 2 m 1 6 mm OD and 0 2 mm ID X2m Required flow rate 50 1rn min Amount of water to be measured 100 m 46 b v Measurement of 130 m min Flow Rate Measure as in the above para i Resistance tube 1 6 mm OD and 1 0 mm ID X 2 m 1 6 mm OD and 0 5 mm ID X2m Required flow Rate 130 2 6 m amp min Amount of water to be measured 260 m2 47 7 PC 30L Interface for SCL 8A Use Excluding P N 228 18888 96 LC 8A 230V CE The unit can be connected to the model
59. s and if an error is detected the audible alarm is given and one of the following error messages is displayed When the alarm is given press the key and the audible alarm is silenced and the error message is substituted by the normal display After that take corrective actions and resume operation ROM or RAM error however can not be corrected ROM FAILURE Atswitching on of power supply ROM is faulty Switch off the power supply of the LC 8A and contact our sales office or agent RAM FAILURE Atswitching on of power supply RAM is faulty Switch off the power supply of the LC 8A and contact our sales office or agent NOT PROTECTED Atswitching on of power supply Data stored in the memories are corrupt In this case the parameters are set to the initial values Press the key and follow the normal operating steps Conceivable causes are lightning surges or discharged memory backup battery ERROR P MAX The liquid transfer pressure exceeds the P MAX setting The pump will be turned off Press the key and check the flow line for clogging Raise the P MAX setting or take other adequate action as required ERROR P MIN The liquid transfer pressure is below the P MIN setting after one minute from starting liquid transfer The pump will be turned off Press the key and check the flow line for leakage or take other adequate action such as raising the P MIN setting ERROR HOME POS The pump driving stepper motor slips and the
60. se care as to the installation site and operation of the preparative liquid chromatograph as a considerable amount of solvent is used 1 Storage and Handling of Organic Solvents Most of the organic solvents used for the liquid chromatograph are hazardous materials controlled by various regulations Store and handle them according to applicable national and local regulations 2 Disposal of Organic Solvents etc Users who do not have their own waste liquid treatment system are recommended to trust the disposal of organic solvents etc to an authorized industrial waste disposal service 3 Ventilation The room equipped with the liquid chromatograph must be ventilated well for the solvents used are mostly flammable and toxic 4 Open Flames Open flames must be kept out of the place where the instrument is installed Avoid installing equipment which generate sparks in the room equipped with the chromatograph Install an adequate fire extinguisher in case of an accident 5 Eye Protectors Eye protectors should be worn where organic solvents are used 6 Other Protective Equipment Washing equipment should be located as near as possible for washing solvent from the eyes or toxic solvent from the skin in case of accident 7 Other Avoid installing the chromatographic equipment in a place exposed to the direct rays of the sun It is also recommended to install the equipment in a room with little ambient temperature variation CONTENTS Introdu
61. sed successively steps are shown in the order of time settings The display returns to the display 2 after all the steps are shown A new step can be input when the display 3 or 4 isshown If the CE key is pressed when the display 4 is shown controlreturns to the display 2 If the key is pressed when the display 2 is shown control returns to the initial display with FLOW PRESS flash ing 0 00 0 100 0 FLOW PRESS PRESSURE P MAX P MIN Y FUNC Press four times 0 00 PROG SETTING Y ENTER gt 0 USED 32 LEFT lt ENTER Input TIME FUNC VALUE Time program ENTER setting mode Input lt 500 FLOW 500 N EA AAA y ENTER Fig 6 7 1 Time Program Setting Process Flow 34 6 7 3 Time Program Setting Steps Description will be made using an example of programming flow rates As outlined previ ously the program set by the instruction FLOW is a linear program For instance if the following program is run when the flow rate is 10 the flow rate is changed as shown in Fig 6 7 2 TIME 15 00 20 00 30 00 FUNC VALUE FLOW 20 00 FLOW 50 00 STOP ml min 50 40 30 20 10 0 10 15 20 30 min Fig 6 7 2 Change of Flow Rate The actual programming steps are 1 Display 2 Keying In Display 3 Keying In Display 4 Keying In Display 10 00 0 100 0 FLOW PRESS PRESSURE P MAX P MIN Flashing FUNC j FUNC FUNC FUNC 10 00 PROG SETTIN
62. sed when liquid in the pump flow path and suction filter is to be exchanged Details in Para 6 4 When the handle is turned counterclockwise OPEN position pumped liquid is led to the drain pipe aD and carrier outlet port 12 d Whereby almost all liquid flows to the drain pipe if a column is connected By turning the handle fully clockwise CLOSEposition pumped liquid flows to the carrier outlet port 12 only Drain pipe connection port Discharges waste liquid from the drain valve Refer to Para 3 6 Mobile phase outlet Pumped liquid delivery port Connected to this port is pipe directed to an injector or column switching valve Line filter Removes foreign objects from carrier Refer to Para 8 2 3 for details of construction Recycle connection port Connect pipe from recycle pipe to this port for recycling Plug port with blind joint when not recycling and during shipping 55 5 2 Flow Line System Pump outlet Waste liquid bottle Mobile pahse Fig 5 2 Flow Line Diagram Suction fdter PREP ASSY Inlet block Plug 1 6P PTFE tube IN ASSY Check valve IN PREP ASSY Pump head Check valve OUT ASSY 228 17421 9 1 228 20054 228 16006 228 20511 91 228 20256 91 228 16809 01 228 09054 93 16 10 micron fdter Inside dia 3 mm outside dia 4 mm including polypropylene bushing Ferrue 1 6F 228 16000 Male nut 1 6MN 228 16001 Pipe SUS316 left 228 22002
63. strong electro magnetic fields are generated near by 3 3 Removal of Transportation Brace The unit is shipped with a brace installed at the bottom of the unit for protecting the mechanical unit of the pump from vibration and shock in transportation Remove the four screws shown in Fig 3 1 4 and remove the brace before installing the unit Remove four Screws Fig 3 1 4 Removal of Transportation Brace 3 4 Connection of Power Supply 1 The unit is availablein the following versions according to the power supply voltage rating P N 228 18888 91 90to 110V 7 5A 50 60Hz P N 228 18888 92 105to 125V 7 5A 50 60Hz P N 228 18888 93 200 to 240V 3 5A 50 60Hz P N 228 18888 94 220to 260V 3 5A 50 60Hz P N 228 18888 95 210 to 250V 3 5A 50 60Hz Ascertain that the voltage marked on the power supply connector on the rear panel matches the voltage of the power supply outlet Operate the unit within the range stated otherwise satisfactory performance will be difficult to achieve Check that the power switch of the main unit is set to OFF on installation 2 Grounding The power supply cord of the unit is a three conductor type including one grounding con ductor Couple the cord to a grounding type three pole outlet for positive grounding 6 If the outlet is a two pole type attach a 2P adapter to the end of the cord In this case the unit must be grounded by connecting a grounding wire to the ground t
64. sy 14 228 20265 9 1 Packing 228 12564 Filter holder 228 17522 01 Line filter assy 2 u 228 18405 91 0000 Fig 8 2 3 3 Line Filter Arrangement 56 8 2 4 Plunger Replacement The plunger surfaces may be scuffed if the leakage is not stopped by replacing the plunger seal or if the service life of the plunger seal is extremely short Check the plunger at first for scuffing If scuffed considerably replace the plunger A conceivable cause of scuffing is rubbing of the plunger surfaces with foreign objects which enter the head or with buffer solute deposits o Plunger Replacement Method Remove the head according to Fig 8 2 11 2 and 3 Remove the Teflon tube from the cleaning flow line Li Q Unscrew the four bolts O Remove the two screws D Hold the plunger with the and remove the head with a phillips screw fingers and pull it out holder by pulling it driver slowly towards the operator forwards 5 Wipe the plunger sur 6 Hold the replacement D Assemble the removed faces well with gauze plunger in gauze and put parts in the reversed order Hold the plunger up to it in slowly of above the light and examine for scuffs with a Aer Fig 8 2 4 Plunger Replacement 57 8 3 Zero Point Adjustment of Pressure Sensor Refer to No 9 Zero point adjuster of pressure sensor in Para 5 1 Front Panel 8 4 Lubrication Lubricate the sliding contact surfaces of the pump at regular intervals f
65. te installation site Be sure to pay due attention to the precautions noted throughout this manual in addition to those below 1 Ventilation Solvents used in high performance liquid chromatograph are often inflammable and toxic therefore the room in which they are used must be well ventilated 2 Fire Never use an open flame in the vicinity of a high performance liquid chromatograph Do not install other equipment that may generate sparks in the same room 3 Protective goggles Wear protective goggles when using solvents 4 Other facilities required A sink should be located as close as possible to the unit so that toxic solvents splashed in the eyes or on the skin may be immediately rinsed off 5 Power source Working voltage range and power consumption of this system are as follows Be sure to connect to the power source conforming to them Part No Rated voltage Capacity Frequency 228 18888 91 100V 750VA 50 60Hz 228 18888 92 115V 750VA 50 60Hz 228 18888 93 220V 750VA 50 60Hz 228 18888 94 230V 750VA 50 60Hz 228 18888 95 240V 750VA 50 60Hz 228 18888 96 230V 750VA 50 60Hz 6 Replacement of fuses This unit uses the following fuse Be sure to replace the fuse of same type and capacity Rated voltage 100 11592 V T Part No 072 01028 10AF 125V 6x 30 Rated voltage 220 230 2409 y T Part No 072 01031 04 7AF 250V 6x 30 7 Repair and maintenance of the unit Normal maintenance of this unit can be performed without removing t
66. ted and the step next to the deleted step takes the place of the deleted step position To delete two or more steps delete them one by one 6 74 Start and Stop of Time Program On completion of program setting the program can be run by the key This key is workable no matter what display is shown Keying In Display PROG RUN light comes on Progress of the program is known by the change of the displays such as FLOW Time since the start of the program can be shown by selecting the auxiliary function Referto the description on MONIT TIME of Para 6 8 AUX FUNC If the STOP instruction is executed the program stops and the PROG RUN display light goes off Press the key for stopping the program forcedly Keying In Display PROG RUN display light goes off Notes The values of the parameters such as the flow rate before the start of the time program are all stored as initial values If the program is stopped the parameters return to the initial values The flow rate and other parameters can be changed by keying in while the program is run ning The parameters are also returned to the initial values also in this case if the program stops Ifa flow rate is keyed in while the flow rate is being changed by the program change in the flow rate may not be made as programmed 6 7 5 LOOP Instruction The whole program can be run repeatedly a specified number of times by the instruction LOOP If the following program is run at a fl
67. th pump 3 Bearing cam broken Replace cam operation is not 4 Belt pulley broken 1 Replace pulley faulty In gradient elution 1 X Zero point of pressure gauge Adjust zero point Para 5 1 pressure readings deviating of two pumps do 2 Line filter of either pump Cleanorreplace line filter Para 8 2 3 not agree clogged Difference below 3 Flow line clogging before Take adequate action 5 kg cm is not joint of two pump flow lines faulty Liquid is not 1 Power supply switch not Turn on power supply switch pumped at all turned on Pumps stand 2 Pump not turned on Press pump key Still LED of pump key not lit Run pump s from SCL 8A 3 Reading of flow rate Set flow rate remains zero Set flow rate from SCL 8A 4 Error displayed Press key P MAX P MIN etc Remove cause of error 5 PC 30L not connected Connect connector Set pump control to local mode Refer toPara 6 6 Pressure does not l Drain valve opened Close drain valve rise 2 Leakage from flow line Take adequate action 3 Flow resistance low 4 Liquid not pumped 65 Pressure rises high 1 Line filter clogged 1 Clean line filter MM Para 8 2 3 Check again by 2 Replace line filter removing column 2 Flow line clogged 1 Take adequate action Pipe inside diameter too small 9 2 Error Messages The LC 8A is provided with some self diagnosis function
68. truction manuals of the other LC 8A system components for the installation and operation of the overall system and those other units 2 Details of Parts The model LC 8A unit comprises the following parts Check the received parts for broken or missing items 1 Model LC 8A unit proper 2 Standard accessory pack P N 228 18214 91 Part P N Q ty Remarks File 085 30023 1 For cutting pipes Drainage SPD 6 228 20063 1 For SPD 6A V Silicon rubber tube 6X 8 016 31350 02 0 8m For draining leaked liquid lt Wrench 8X 10 086 03006 2 For connecting pipes Wrench 13X 17 086 03017 1 For replacing outlet check valve and line filter Adjustable wrench 086 00004 1 For replacing inlet check valve Securing fittings 228 20502 2 For securing unit Syringe L208 046 00038 01 1 STingeneedle RefertoPara 63 T NM 228 18216 91 1 PTFE tube 2X 0 5 016 37503 4m Connected to seal cleaning line Pipe SUS316TP 1 6X 0 8 011 30791 02 2m Seal 2004 228 18306 2 Seal of cleaning line Refer to Para 8 2 1 Seal UR304 228 18092 2 Pump seal Refer to Para 8 2 1 Male nut 228 16001 3 Connecting pipes Refer to Para 3 3 Ferrule 228 16000 3 Connecting pipes Refer to Para 3 3 Lubricating oil 228 09408 92 1 Refer to Para 8 5 Spacer 228 20047 2 Refer to Para 3 1 Allen wrench 228 03805 1 Refer to Para 8 2 1 Line filter assy 228 18405 91 1 Refer to Para 8 2 3
69. turned to the reservoir the suction filter may be clogged with the particles 3 9 Other Use the supplied lock catch for securing Teflon pipes such as the drain pipe and the suction filter The application method of the supplied SPD 6A V drainage is outlined in the instruction manual of the LC 8A system 11 4 Specifications 1 Pump Type 2 3 A nN Ut i A 60 m 2 NM 6 Ln HA 12 13 a b C Double plunger reciprocating pump delivering 250 u by one stroke per head Liquid Transfer Characteristics Constant Flow Rate Transfer Flow rate range In steps of 0 1 m min Accuracy in flow rate setting Flow rate stability Other Functions 0 1 to 50 m2 min 10 to 300 kg cm 0 1 to 100 m min 10 to 180 kg cm 0 1 to 150 m min 10 to 80 kg cm 2 0 5 to 150 m2 min measured with water 0 5 0 5 to 150 m min Pulsation correction function 30 m amp min or below Flow rate correction function for liquid compression ratios Constant pressure liquid transfer mode Time program Flow rate pressure setting events 1 2 3 SVO 1 2 Monitoring functions Flow rate pressure plunger position integrated number of revolutions Self diagnosis function of liquid transfer conditions Pressure limiters upper limiter and lower limiter Cleaning line for washing away buffer Diaphragm strain gauge type pressure gauge Manually operated d
70. wo pump heads is excessively low o INC and DEC are displayed at normal pump start up stop and others o Conceivable causes for X XX L are a Airis trapped in the left pump head b Liquid leaks from the seal or else from the left head c The inlet check valve of the left head or the outlet check valve of the right head is faulty Read the right as the left and the left as the right in the above a b and c when X XX R is displayed Function Sets ON and OFF of the solenoid valves of the FCV 130AL option Setting ON OFF of the two solenoid valves of the FCV 130AL is controllable Key in the number of the solenoid valve to be turned on lt Example gt To turn on the solenoid valve 1 1 To turn on the solenoid valve 2 2 To turn off both valves 0 EVENT Function SetsON OFF of the relay contact at the REMOTE connector on the rear panel and ON OFF of the cleaning pump of the FCV 130AL Setting 1 and 2 are assigned to the relays refer to Para 5 5 and 3 to the cleaning pump Key in the numbers of the parts to be turned on lt Example gt o To turn on relay 1 o To turn on relay land the cleaning pump of the FCV 13OAL Jar o When EVENT land 2 are displayed key in and for turning off relay 1 only and key in o and ENTER for turning off all EXT S Function Uses the relay 1 contacts EVENT 1 of the REMOTE connector as the start signal output of the time prog

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