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KR4i Large Capacity Refrigerated Centrifuge

1. Thermo Fisher Scientific Using the Product Chapter 5 XOJ9A V UOUA uiniuelr 9ZJ91 uoper 99 S ssojureis auojinsAjod euejAdoud AJod aus Ayl9 Al0d ayeuoqiedAjod 09dd 1euiojeAjod uoJ N AION Jeu M uujeg 9 EJIIN 9SO N 9D 9 eJ1 mq ojejooe eso nijo2 N eung uiniuiun v Jenae Thermo Fisher Scientific O U U Sd St Mt Sd Mt Mt St St S Mt Mt Sd Sd Mt Sd Mt St Mt Sd Mt Mt O U U O KR4i M M M M M U M M M S X O U O U M U O S U Caesium Acetate Caesium Bromide Caesium Chloride Caesium Formiate Caesium lodide Caesium Sulphate Caesium Trifluoracetate Calcium Carbonate Calcium Chloride Calcium Hypochlorite Calcium Sulphate Carbazole Carbon Sulphide Carbon Tetrachloride Cedarwood Essence Chlorine dry Chlorine moist Chloroacetic Acid p Chloroacetophenone Chlorobenzene Chloroform Chlorosulphonic Chromic Acid 5 Chromic Acid 10 Chromic Acid 50 Cinnamon Essence Citric Acid 10 Copper Nitrate Copper Sulphate Croesol Cyclohexane Cyclohexanol Cyclohexanone Cyclopentane Decane 5 28 Using the Product Chapter 5 XOJ9A V UONA uiniuelr 9ZJ91 uoper 99 S ssojureis euojinsA od euejAdoud AJod aus Ayl9 l0d ayeuoqiedAjod 09dd 1euiojeAjoq uoJ N J JON J2U Yy uujeg 9 EJIIN 9so nijo2 9 eJ1 mq
2. Electric batteries and accumulators situated in the front panel of the machine among the other electrical and electronic com ponents Oil in the compressor of the refrigeration system Refrigerant Disposal of these elements must be in accordance with applicable regulations and recommendations defined by the relevant regula tory bodies In addition these elements must be disposed of differ ently based on the different noxious substances they contain possibly by a specialized disposal company KR4i 7 1 Chapter 7 Disposal of Product 7 2 KR4i Thermo Fisher Scientific Thermo Fisher Scientific Warranty conditions Annex A Warranty conditions The warranty period begins on the day of delivery During the war ranty period the centrifuge will be repaired or replaced at no charge if production or material defects can be demonstrated The prerequisite for warranty claims is that the centrifuge is used for the purpose intended as described in this operation manual and that installations enhancements adjustments alterations or repairs are carried out only by persons authorized by Thermo Fisher Scientific to perform the regularly required maintenance and care KR4i A 1 Warranty conditions A 2 KR4i Thermo Fisher Scientific Great Britain Deutschland Thermo Fisher Scientific France Espana Declaration of conformity Annex B Declaration of confor mity WEEE Conformity This product is
3. MAKE CERTAIN THAT THE POCKETS ARE WELL DRIED Thermo Fisher Scientific Chapter 5 Using the Product Contamination Hazard Thermo Fisher Scientific For swing out rotors be sure to clean the grease from the trun nions and replace it with a small amount of fresh grease This will ensure that the buckets swing freely The vast majority of apparent imbalance problems arise from the failure of the user to clean and grease the trunnions and not from instrument error Pay particular attention that no deposit remains in the bottom of the pocket because the pressure of a flask or tube from above during centrifugation will significantly increase the chances of corrosion NEVER LEAVE A DAMP ROTOR ON A METAL SURFACE PAR TICULARLY STAINLESS STEEL because an electrochemical reac tion could take place with the aluminium or magnesium in the rotor Our centrifuges are likely to be used in medical research where hazardous substances including radioactive chemicals are fre quently found see also Biological risks ALWAYS USE THE APPROPRIATE DECONTAMINATION PRO CEDURES WHERE THE ROTOR IS EXPOSED TO THESE CHEMICALS Dangerous substance It is recommended that the user takes all the necessary precautions when centrifuging substances known for their toxicity or radioactivity or when they are contaminated by microbiological pathogens notably material of Risk Group Il as identified in the Laboratory Biosafety Manual edited by the World
4. Fus z o 9 c o o O E 9 9 rc NH E 5 5 9 amp 9 2 sS x EZ T E S JEEE AO ks a aie cy S E 9 8 S 8 o 3 5 vo o o gt O gt o O O 0 o gt o 2 o 25130 0 O 6O X 22262242085 2 Trichloroacetic Acid U O O O O O O O Mt Mt Mt M S O O O O O O 1 2 4 Trichlorobenzen O O O O O O O O U U U U O O O O O O Trichloroethane S U S O M S O S U U U M O S S S S S Triethylamine O O O O O O O O U O U O O O O O O O Triethylene Glycol O O O O O O O O S Sd S S S O O O O O O Tris Buffer neutral S O O O O O O O S S S S S O S S O O O Trisodium Phosphate O O S O M O O S S O S S O O S S S S O Triton X 10 S OOOO O O O S S S S S O S M O O O Turpentine S O O O O O O O Sd Mt St Sd U O O O O O O Undecyl Alcohol Oo O O O O O O O Sd Sd S Sd M O O O O o o Urea M O S S S O O S S S S S S S S S S O O Urine O O S O S O O S S S S S O O S S S o O Vinylidene chloride O O O O O O O O U U U U U O O O O O O Xylene S U P O M S O U U U U U U S S S S S M Zinc Chloride M S S O O O O S S S S S S M S S S S S Zinc Hydrosulphite U O O O O O O O O O O O O O O O O O O Zinc Sulphate U O O O O O O O Gd O S Sd O O O O O O O Polypropylene Copolymer This table is intended as a guide only because of the difficulty in quantifying cross checking and monitoring the results under differ ent conditions of temperature pressure and purity relating to the solvents and samples dissolved therein It is strongly recom mended that you carry out your own trials particularly before pro trac
5. Introduction Switching On Precooling Standard Use KR4i The VIDEOset graphics based control software is designed to com bine power with simplicity VIDEOset includes on screen guidance and intuitive programming The screens presented in this manual could differ slightly according to the version of the software Those who use this centrifuge must be well trained according to the instructions in this manual and according to the rules of the institu tion where the centrifuge is used It is the responsibility of the pur chaser to ensure that users are trained This centrifuge should only be used in a work area that is lighted to the requirements of the local labor code Immediately after switching on the screen displays the Thermo Fisher Scientific logo then the stand by screen is displayed show ing the number of the last application program used and its basic parameters A keypress on CE will cause the display of the value of the actual temperature of the air in the bowl to the left of the set value during the time that the CE key is pressed If the operator wishes to precool the bowl and rotor he simply selects PRE COOL having installed the rotor and the Jouan KR4i runs according to the pre set values which are indicated on the stand by display The display now indicates PRECOOL ON The remaining precool time real temperature and the dynamic rotation symbol are dis played Uniquely during a PRECOOL run it is p
6. directive UE pour les d chets d quipements lectriques et lectro niques directive DEEE 2002 96 Ceci est caract ris par le sym bole ci contre Thermo Fisher Scientific a sign des conventions avec les entreprises de recyclage et d limination dans tous les pays membres de l UE en ce qui concerne le recyclage ou l limi nation de l appareil Indications concernant les entreprises de recy clage et d limination en Allemagne et informations sur les produits de Thermo Fisher Scientific qui sont concern s par la directive RoHS angl Restriction of the use of certain hazardous substances in electrical and electronic equipment Restriction de l utilisation de certaines substances dangereuses dans les quipements lec triques et lectroniques que vous pouvez consulter sur Internet l adresse www thermo com WEEERoHS Conformidad con WEEE Este producto cumple con las disposi ciones de la Directiva europea sobre equipos el ctricos y electr ni cos usados Directiva WEEE 2002 96 Ello se indica con el KR4i B 1 Declaration of conformity Italia Nederland KR4i simbolo al margen Thermo Fisher Scientific tiene firmados acuer dos con varias empresas de reciclaje y eliminaci n de residuos de todos los estados miembros de la U E respecto a la reutilizaci n y la eliminaci n de este aparato Para obtener informaci n sobre las empresas de reciclaje y eliminaci n de residuos en Alemania as como sobre los
7. without lid 12 quin or quad bags Designed for multi purpose uses blood banking 12 quin bags or quad bags large volume processing 6 x 1000 ml biochemistry cylindrical or rectangular plastic inserts and microtitre carriers The maximum authorized speed whatever accessory used is 3800 rpm without lid It can be equipped with a lid in the case of blood banking buckets Cat No 11178155 and cylindrical buckets Cat N S 11178136 11178135 11178152 Performance can then reach a speed of 4250 rpm 5735 x g RHKAB 2 place swing out rotor Designed for RIA usual RIA counter racks can be directly centri fuged without transferring the tubes see tables Maximum RCF is 3300 rpm 2301 x 9 Blood Banking 11178088 Set of 4 Large Volumes 11178143 Set of 4 11178158 with partition 2 quin bags 41193596 PC 1600 ml 11178159 without partition 2 quin bags 41193600 PP 1600 ml 11178170 2 bags with filter in line RPM RCF RAD RPM RCF RAD without rotor lid 4000 4758 266 4000 4883 273 with rotor lid Table 1 2 RP6 Biochemistry 11178138 Set of 6 4250 5372 266 Biochemistry 11178136 Set of 6 4250 5513 273 Biochemistry 11178140 Set of 6 11178216 1 piece 11178135 Set of 6 Complete with transparent cap amp stainless steel tray also accepts two rectangular plastic inserts 11178190 1 piece See detailed table RPM RCF RAD RPM RCF RAD RPM RCF RA
8. 0 to 9 and CE clear entry enables parameter values to be keyed in directly when required f 3800 em 24 EPL IE NEST 00 15 nemn ou COOL lt MODE NEW amp SAVE APPLI Control Panel Figure 5 1 KR4i 5 3 Chapter 5 Using the Product 5 4 KR4i Certain key functions are very common others are found only on a dedicated screen They operate as follows KR4i Increases the value of the indicated parameter Sustaining pressure on the key causes a rapid increase Changes the arithmetical sign of the current figure from positive to negative or vice versa E Decreases the value of the indicated parameter Sustaining pressure on the key causes a rapid decrease V Moves the highlight bar down a list in order to make a selection AV Moves the s indicator ffom one parameter to another to allow values to be altered ABOUT INTEGRAL Shows a graphical representation of the integral together with values calculated from the previous run where possible ACC Enables the acceleration rate to be altered between the 10 choices from O slowest to 9 fastest BASIC DISPLAY Switches the display from the fully detailed run screen to the basic run screen with 3 fundamental parameters BRK Enables the braking rate to be altered between the 10 choices from O weakest to 9 strongest CONFIG Provides access to fundamental set up choices such as langua
9. 10 Temperature Range 20 to 40 C Step 1 Accuracy 11 5 C Motor Induction Display High visibility digital display 240 x 128 pixels Control Microprocessor 16 bit Memory size 24 programs Program protection Individual password Winshielded rotor RP4 Cat N 11178250 Bucket Cat N Max Max Bucket Load Load weight speed RCF speed RCF weight kg density kg bucket with lid without lid N4C 1600 ml 11178143 4250 rpm 4000 rpm 1 671 1 2 2 13 Cylindrical 5513xg 4883 xg N4T Transfusion 11178088 4250 rpm 4000 rpm 2 025 1 1 1 679 5372 xg 4758 xg At bucket maximum speed Winshielded rotor RP6 Cat N 11178252 Bucket Cat N Max Max Bucket Load Load weight speed RCF speed RCF weight kg density kg bucket with lid without lid 1 4 KR4i Thermo Fisher Scientific Chapter 1 Presentation of product Winshielded rotor RP6 Cat N 11178252 N6CD Cylindrical 11178152 4250 rpm 3800 rpm 1 375 1 2 1 5 Hard anodised 5735 xg 4585 x g N6CE Cylindrical 11178135 4250 rpm 3800 rpm 1 375 1 2 1 5 Sealed 5735xg 4585 xg N6B Biochemistry 11178138 4250 rpm 3800 rpm 1 54 1 2 1 4 Rectangular open 5392xg 4310 xg N6BMT Rect 11178140 4250 rpm 3800 rpm 1 54 1 2 1 4 Sealed uT 5392 xg 4310 xg N6T 11178155 4250 rpm 3800 rpm 1 575 1 1 1 64 Transfusion 5674xg 4536 x g Winshielded rotor RC6 Cat N 11178254 Bucket Cat N Max speed Maxspeed Bucket Load Load weight RCF with RCF weight kg density kg bucket lid with
10. Butyl Ketone Methyl Ethyl Ketone Methylene Chloride Mineral Oil Nickel Chloride Nickel Salts Nickel Sulphate Nitric Acid 10 Nitric Acid 20 Nitric Acid 50 Nitric Acid 95 Nitric Acid conc Nitrobenzene Octane Octyl Alcohol Oleic Acid Orange Essence Oxalic Acid Thermo Fisher Scientific Using the Product Chapter 5 XO 9A V UOHNA uiniuelr IezjeL uoper 99 S SSAJUIBIS SUOJNS JOd euejAdoud Ajod aus Ayl9 Al0d ayeuoqiedAjod 09dd 1euiojeAjog uoJ N J JON J2U Yy uujeg N SONJI nq ojejooe eso nja N eung uiniuiunj v Jenae Thermo Fisher Scientific Sd St St St Sd S St St St St Sd St Sd St St St Sd O O O M O M M O KR4i U X O O O U M M U U U S S O O M Perchloric Acid 10 Perchloric Acid 70 Phosphoric Acid 10 Potassium Carbonate Potassium Hydroxide 5 Ozone Paraffin Pentane Perchlorethylene Perchloric Acid Petrol Phenol 5 Phenol 50 Phenol crystals Phenol liquid Phenyl Ethyl Alcohol Phosphoric Acid conc Picric Acid Pine Oil Potash conc Potassium Bromide Potassium Chlorate Potassium Chloride Potassium Hydroxide conc Potassium Nitrate Potassium Permanganate Propane Gas Propionic Acid Propyl Alcohol Propylene Glycol Propylene Oxide Pyridine Resorcinol Sat d Sol Rubidium Bromid
11. Health Organisation Centrifugation of Risk 2 microorganisms and materials 1 Centrifuge tubes and specimen containers for use in the centri fuge should be made of thick walled glass or of plastic and should be inspected for defects before use 2 Tubes and specimen containers should always be securely capped 3 The interior of the bowl should be inspected daily for staining or soiling at the level of the rotor If this is evident then the centrifu gation protocols should be re evaluated 4 Angle heads should not be used in microbiology except in spe cial high speed centrifuges With ordinary angle heads some fluid even from capped tubes may be ejected because of the geometry of the machine 5 Except in ultracentrifuges and with small prothrombin tubes a space of at least 2 cm should be left between the level of the fluid and the rim of the centrifuge tube KR4i 5 23 Chapter 5 Using the Product 5 24 KR4i Examples of commonly used techniques are outlined below The information is given as a guide only It is the responsibility of the owner to use the most suitable procedure The rotor should always be completely disassembled before being subjected to heat and after external chemical cleaning Seals tubes and plastic compo nents should be decontaminated Disinfection Alcohol 70 ethanol or isopropanol applied for 10 minutes is ideal for bacteria and viruses Autoclave rotors for 20 minutes at 120 C to
12. Leave the protocol temperature at 4 C The centrifuge will control the air at 1 C The air temperature will be measured independently 1 C then the value of AT will be added here 3 C to give the dis played temperature of the sample 4 C KR4i 5 19 Chapter 5 Using the Product 5 20 Caution Rotor and Accessory Precautions Corrosion Information KR4i In the case where an elevated temperature has been selected and the measured temperature attained at the run end is below the set value e g set value 30 C attained value 28 C then enter a nega tive value for AT here AT 2 C The following precautions must be observed by the operator Ensure that the accessories are correctly fitted and balanced e Check that the rotor is fully locked onto the drive shaft Check that the rotor lid fittings are screwed on tightly Check that there is no water nor other liquid in the base of the windshield Check that all of the buckets are in place and that they swing freely Check that there are no empty transfusion liners in the buckets Do not attempt to override the lid interlock system Do not try to open the lid until display returns to the stand by mode shown when machine is switched on Use only a properly grounded mains power socket Special attention is required to the following Installation of the unit proper ventilation levelling of the centri fuge e Accessory handling rotor
13. Requirement osas RE eb oe RE Dindi 4 4 POSITIONING serials 4 5 Lid Opening Manual Lid Unlocking 4 7 ASPE mr P 4 9 Rotor Installation c ccccceececeeeeeeeeeeeeeenneeeeeeeeeeeeeeeeeeees 4 9 Power Off Operation Stc 4 11 Chapter 5 Using the Product eere 5 1 Handling of Transfusion israel 5 1 ACCOSSOMES M 9 5 1 Balancing The Load ore renti afinada nea iet s 5 2 Controls and Indicators oooooooccccccnncnccocacccicccnnnncnccnnnnnnnnnos 5 3 Po 5 6 faves sl elf olg eleme PR O EE 5 14 KR4i i Table of contents BRAKING cu db pie D dd GALO elec dd 5 15 Integration acce te ipt Dope PE E A enda 5 16 Refrigeration System 5 19 e iW eU 5 20 Rotor and Accessory Precautions suuusse 5 20 Chemical Compatibility Table for Rotors Tubes and ACCESSOTOS sur ucro ciue ba e ainda geada 5 26 Machine ALANS ooo eet eec el Quia 5 34 Operational Limitations eee eeen erenn renren 5 36 Expected bucket life ists eiie titer reete 5 36 COMO IO Mirar ri da ic Coe aou s in ue vb ob OR uds 5 37 Chapter 6 Maintenance euren rennen 6 1 Daily Ioab isos t one Rawat ceed tenet 6 1 Weekly GleanihQgu ite exo ci eoe inre reae SES Pare AER 6 1 Periodic Preventive Maintenance s 6 2
14. Stop button shutdown e Overtemperature shutdown KR4i 6 3 Chapter 6 Maintenance 6 4 Verification of adjustments Rotor Removal KR4i e Circuit breaker shutdown Condition of springs Lidlock springs e Lid support springs Recognition of abnormal play in mechanical systems Lid opening process Motor bearings Condition of pieces susceptible to wear Bearings Carriers adapters linersa Buckets Rotors Adjustment of end of run systems Lid lock mechanisms Lid open detector Verification of condition of indicators Condition of measurement systems Tachometer Other measurement systems temperature etc Condition of displays Read outs Indicator lights Changing the type of rotor on the Jouan KR4i must be carried out by qualified and authorised personnel given the adjustment of overspeed control necessary for the type of rotor installed Please consult your Thermo Fisher Scientificsupplier for any operations of this nature Thermo Fisher Scientific Chapter 6 Maintenance Fuse Replacement LID Opening Manual LID Unlocking Thermo Fisher Scientific Changing the type of rotor on the Jouan KR4i must be carried out by qualified and authorized personnel given the adjustment of overspeed control necessary for the type of rotor installed Please consult your Thermo Fisher Scientific supplier for any operations of this nature Using the wrench supplied remove the ro
15. U O S S O O S S S O S Aluminium Chloride O O S S O S O S S S S O U S S S O O Aluminium Fluoride O O O O O S O S S U S S O O S O S O O Aluminium Hydroxide S O O O O O O O S Mt Sd S S O O O O O O Aluminium Nitrate M O O O O O O O S O O S O O O O O O O Aluminium Sulphate S O O O O O O O Sd O S Sd O O O O O O O Amino Acids S O O O O O O O S S S S O O O O O O Ammonia S O O O O O O O U S Sd O O O O O O 5 26 KR4i Thermo Fisher Scientific Using the Product Chapter 5 XOJ9A V UONA uiniuelr 9ZJ91 uoper 99 S ssojureis euojinsAJod euejAdoud AJod euoej uieAJod ayeuoqiedAjod 09dd 1euiojeAjoq uoJ N J JON Jeu M uujeg 9 J2MIN 9so nijo2 ojeJAqnq ojejooe eso nija N eung winiuiwiniy Jenae 5 27 O KR4i U St Sd Sd Sd sD St Sd S S S U Mt Sd Mt St Sd Sd Sd Sd U O O O S O O S M O U S O U S St Ammonium Acetate Ammonium Carbonate Ammonium Chloride Ammonium Hydroxide 10 Ammonium Hydroxide conc Ammonium Oxalate Ammonium Phosphate Ammonium Sulphate Ammonium Sulphide n Amyl Acetate Amyl Alcohol Amyl Chloride Aniline Aqua Regia Barium Chloride Barium Hydroxide Barium Sulphate Benzaldehyde Benzene Benzoic Acid Sat Benzyl Acetate Benzyl Alcohol Boric Acid Bromine Bromobenzene Bromoform Butadiene Butane n Butanol n Butyl Acetate Butylene Butyl Chloride Butyric Acid
16. application and releasing of force as in the number of runs translates after a given number of runs into a very rapid reduction in mechanical strength The item cans no longer resist the same force as at the start of its life If the force is applied repetitively with same intensity it will rupture It is this phenomenon of excessive fatigue which is used when repeatedly bending and unbending a piece of wire or plastic in order to break it Each centrifugation run is a fatigue run for the buckets Bucket lifetime Rotors and buckets are marked with maximum lifetime ratings in years and max number of cycles In no case should these accesso ries ever be used past the lifetime for which they are rated Ignoring the ratings exposes the user to serious danger Remarks The graphs show that soft spin runs such as for obtain ing PRP and for platelets from the buffy coat up to 1500 x g or about 2200 rpm do not incur any fatigue So the number of runs carried out at low speed is not counted in the total for the calcula tion of the lifetime of the buckets The above graphs have been established from tests carried out on samples of metal in perfect condition and over a period of many hours KR4i 5 37 Chapter 5 Using the Product 5 38 Electrical risks Improper KR4i As the usable lifetime of the buckets is several years it is important to take into account the wear due to corrosion to which the buckets may have been sub
17. approximate acceleration times according to rotor type and maximum speed Curve 0 1 2 3 4 5 6 7 8 9 Threshold 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 RP4 cylindrical buckets 7 min 6 min 6 min 5 min 5 min 4 min 4 min 3 min 3 min 2 min without lid N4C 1600 ml bottle 10sec 40 sec Osec 40sec 10sec 40sec 10sec 30sec Osec 40sec 4000rpm d 1 0 RP4 cylindrical buckets 7 min 6 min 6 min 5 min 5 min 4 min 4 min 3 min 3 min 2 min with lid N4C 1600 ml bottle 10sec 30 sec Osec 40sec 10sec 40sec Osec 30sec Osec 40sec 4250 rpm d 1 0 RP6 N6T buckets polypr 7 min 6 min 6 min 6 min 5 min 4 min 4 min 3 min 3 min 2 min with lid liner without partition 20sec 40sec 10sec Osec 20sec 50sec 10sec 40sec 10sec 50sec 3800 rpm triple bags d 1 0 RP6 N6T buckets polypr 7 min 6 min 6 min 5 min 5 min 4 min 4 min 3 min 3 min 2 min with lid liner without partition 10sec 40sec 10sec 50sec 20sec 50sec 10sec 40sec 10sec 50sec 4250 rom triple bags d 1 0 RC6 N6C buckets 7 min 6 min 6 min 6 min 5 min 4 min 4 min 3 min 3 min 3 min without lid adaptor for bottle 20sec 50sec 20sec Osec 30sec 50sec 20sec 50sec 20sec 0 sec 4300 rpm 1000 ml bottle d 1 0 RC6 N6C buckets 7 min 7 min 6 min 6 min 5 min 5 min 4 min 4 min 3 min 3 min with lid adaptor for bottle 40 sec Osec 30sec 20sec 40sec 10sec 40sec Osec 30sec 10sec 4900 rpm 1000 ml bottle d 1 0 RHK4B NHK4B buckets 6 min 6 min 5 min 5 min 4 min 4 min 3 min 3 min 2 min 2 min 3300
18. be controlled at a lower level than the ambient temperature The measured value of air temperature in the bowl is displayed and the temperature is con trolled by the microprocessor which controls the cooling unit The operator can use the temperature compensation setting so that the sample temperature is more accurately controlled accord ing to the user request by measuring the difference between the sample temperature obtained during the run and the set value The cooling unit of the Jouan KR4i has either an air cooled con densor or a water cooled condensor Air cooled condensor Accumulation of dust on the condensor fins will seriously reduce the refrigeration power Every 3 months disconnect the unit from mains remove the front panel and clean the condensor with a vac uum cleaner and a brush Water cooled condensor In this configuration the unit is provided with a water pressostat which controls the input of fresh water Cold water 10 C and less as well as warm water 25 C and more reduce the efficiency of the cooling group Determination of the temperature compensation value AT To determine the temperature compensation value AT to be pro grammed carry out a run as normal with AT 0 C no compensa tion applied At the end of the run measure the sample temperature with a thermometer If for example the set temperature was 4 C and the measured temperature is 7 C then program the AT as 3C for future runs
19. buckets e Cleaning of the accessories of the rotor chamber lubrication of the trunnions Centrifuge ware shape material and quality of the vessels must be in accordance with the performance of the centrifuge Load balancing Samples cleaning of the accessories is particularly necessary when using corrosive products in the samples saline acids bases Ensure that the set point speed is not more than the maximum speed inscribed on either the rotor or the buckets Thermo Fisher Scientific rotors made of aluminium alloy are designed to operate at their rated RCF for many years With careful use they will resist corrosion lessening the possibility of excessive imbalance disruption and subsequent damage to the instrument The primary conditions for the initiation of corrosion exist in every laboratory during daily use of the centrifuge For this reason it is essential that due care and attention be paid to inspection and cleaning Thermo Fisher Scientific Chapter 5 Using the Product Thermo Fisher Scientific Chemical Corrosion This corrosion is characterized by chemical reactions due to the existence of an electrolyte liquid on the surface of the item If these substances are allowed to remain on the surface the corrosion will almost certainly occur This produces first a discoloration of the anodisation then pitting of the metal Aluminium is a metal which easily reacts with ionic solutions Anodisation produc
20. integral value NEW APPLI Gives access to the selection of new parameter values starting from a set of default values NONE Selects NO PASSWORD PROTECTION when memorizing an application PRE COOL Starts a predetermined precooling cycle 2000 rpm 4 C 15 min During this cycle all other programming facilities are operational RADIUS Enables the effective radius of a selected rotor to be modified in order to allow for the use of an adaptor or to select a boundary at which a set g force can be applied ROTOR Lists the available rotors from which a selection can be made with their capacity and performance SAVE APPLI Allows the current set of parameter values complete or incomplete to be stored in one of 32 memories with or without an individual password SCROLL APPLI One press displays all parameter values of next used memory Continued presses will display the values for all memorised applications Also acts as application program selection key START Starts the run STOP Stops the run overiding any programmed time or integral Note If the instrument remains switched on but not used then 5 minutes after the end of the last run the screensaver operates The backlight is extinguished although the display can still be read Press any key to light up the screen then continue with normal operation KR4i 5 5 Chapter 5 Using the Product 5 6 Use
21. outside of the windshield if necessary Next take a clean rag and apply the mild water detergent solution to it Reclean the surfaces of the bowl and the windshield with this rag Next take a dry rag and remove all traces of the solution from the surfaces b The rotors and buckets should be washed with warm water at lt 50 C and a mild soap or detergent for example a household detergent for handwashing KR4i 6 1 Chapter 6 Mai ntenance Periodic Preventive Maintenance Suggested Template for Annual Centrifuge Safety Inspection Visual verification of equipment KR4i Rinse the rotor thoroughly with preferably distilled water Dry the rotor or bucket and preferably with hot compressed air store upside down c The Jouan KR4i centrifuge is equipped with a drainage hose attached to the rotor chamber to enable rinsing the chamber when required To rinse and drain operate the unit at 3000 rpm at 20 C temper ature for 1 minute The following preventive maintenance procedures are required for the Jouan KR4i centrifuge Ensure that the positioning instructions as described in chapter 2 5 are always carried out horizontability anti skid pads cas tor positioning Check the condition of all accessories rotors buckets inserts sealed lids Check earth continuity protection when undertaking any inter vention inside the machine Stability Is the centrifuge level Is the centrifuge stab
22. removing the old grease first with a clean tissue Note Never mix buckets from different sets on the same rotor If the mes sage persists call an engineer LID unlocked Displayed if the lid was not correctly shut The instrument will inform you to close it correctly in order that it can start the requested run Tachometer failure Displayed when an unrealistic speed situation is detected For example if the measured speeds in two sequential measure ment steps are very different or if a power cut occured or if the unit was switched off momentarily during a run in which case speed KR4i 5 35 Chapter 5 Using the Product 5 36 Operational Limitations Expected bucket life Mechanical strength KR4i would be detected immediately upon switch on If speed is read able certainly true in the later case its real value will be displayed and in every case a TIME OUT safety lock will be applied prevent ing the user from opening the lid for a set period after the speed reaches zero or at least effects this lock out period in the case of no detected speed Motor overtemperature When using the centrifuge in bad condi tions e g motor failing a sensor detects an overtemperature in the motor and the run is automatically stopped Chamber overtemperature When using the centrifuge in bad conditions or if the refrigeration system is failing the chamber tem perature can seriously increase depending on the speed level and
23. subject to the regulations of the EU Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 It is marked by the symbol opposite Thermo Fisher Scien tific has entered into agreements with recycling and disposal com panies in all EU Member States for the recycling and disposal of this device For information on recycling and disposal companies in Germany and on the products of Thermo Fisher Scientific which fall under the RoHS Directive Restriction of the use of certain haz ardous substances in electrical and electronic equipment please visit the website www thermo com WEEERoHS WEEE Konformitat Dieses Produkt unterliegt den Bestimmungen der EU Richtlinie f r Elektro und Elektronik Altger te WEEE Richtlinie 2002 96 Es ist durch das nebenstehende Sym bol gekennzeichnet Thermo Fisher Scientific hat mit Verwertungs und Entsorgungsbetrieben in allen EU Mitgliedstaaten Vereinba rungen zur Wiederverwertung oder Entsorgung dieses Ger tes getroffen Angaben zu Verwertungs und Entsorgungsbetrieben in Deutschland sowie Informationen zu Produkten von Thermo Fisher Scientific die unter die RoHS Richtlinie fallen engl Restriction of the use of certain hazardous substances in electrical and electronic equipment Beschr nkung der Verwendung bestimmter gefahrli cher Stoffe in Elektro und Elektronikger ten finden Sie im Inter net unter www thermo com WEEERoHS Conformit DEEE Ce produit est soumis aux dispositions de la
24. symbol opposite refers to general hazards WARNING means that injuries or material damage or contamination could occur ATTENTION means that material damage could occur Thermo Fisher Scientific Description Relative Centrifugal Force Centrifugation formulae Thermo Fisher Scientific Chapter 1 Presentation of product The Jouan KR4i centrifuge is designed to separate substances of different density by centrifugal force Different rotors can be fitted to the drive in order to match the sam ple containers and the required performance The maximum speed and by consequence the resultant centrifugal force is a function of the rotor in use A control system permits the speed acceleration and braking rates run time and temperature to be monitored and controlled The refrigeration is assured by a refrigerant group charged with a CFC free fluid The refrigerant fluid circulates around the bowl to achieve maximum efficiency The Jouan KRdi is mainly dedicated to the following application fields Blood separation processing Large volume industrial processing Biochemistry Radio immune assay Suspended solids are separated by the Relative Centrifugal Force RCF applied to the sample The effective force increases with the square of the speed of rotation and the distance from the axis of rotation The following formulae permit the calculation of primary parameters and of transformations relating to changes in p
25. top speed of the rotor and the related RCF allowing for actual radius After all the required parameter changes for this screen have been made select ENTER to save the changes and return to the simple programming screen which could indicate control by RCF B Temperature related Temperature compensation AT negative temperature On the simple programming screen use V to move the flashing arrowhead s to indicate the temperature then select DETAILS The flashing arrowhead indicates AT For an explanation of AT Use the keypad to input a value and then if required the key to change the mathematical sign of the value Alternatively the user has the choice of directly selecting one of the most popular operating temperatures 4 20 or 37 C and or using to introduce a negative value If wished use AV to first indicate temperature selection then select the temperature Select ENTER to return to the simple pro gramming screen C Time related Integral hold On the simple programming screen use AV to move the flashing arrowhead s to indicate the time then select DETAILS The flashing arrowhead indicates integral selection For more infor mation on integral select About integral and also More info for an accurate definition KR4i 5 9 Chapter 5 Using the Product Saving an Application Password Protection Overriding Password Protection 5 10 Erasing A Program KR4i Select ESC ESC to return to t
26. when working with corrosive samples salt solutions acids bases the accessory parts and vessel have to be cleaned carefully Always balance the samples Centrifuging hazardous substances Do not centrifuge explosive or flammable materials or sub stances which could react violently with one another The centrifuge is neither inert nor protected against explosion Never use the centrifuge in an explosion prone environment Do not centrifuge toxic or radioactive materials or any patho genic micro organisms without suitable safety precautions When centrifuging microbiological samples from the Risk Group II according to the Laboratory Bio safety Manual of the World Health Organization WHO aerosol proof biological seals have to be used For materials in a higher risk group extra safety measures have to be taken If toxins or pathogenic substances have gotten into the centri fuge or its parts appropriate disinfection measures have to be taken Highly corrosive substances which can cause material damage and impair the mechanical stability of the rotor should only be centrifuged in corresponding protection tubes IF A HAZARDOUS SITUATION OCCURS TURN OFF THE POWER SUPPLY TO THE CENTRIFUGE AND LEAVE THE AREA IMMEDIATELY Proper use The centrifuge is to be used for separating materials of different density or particle size suspended in a liquid Maximum sample density at maximum speed 1 2 2 cm The
27. 15 15 oa 23 HIMN EK AP 4 C TA Iwoore EXPAND STOP A Thermo Fisher Scientific KR4i 5 7 Chapter 5 Using the Product Programming Of Parameters Basic parameters speed temperature time Advanced parameters KR4i EE 3800 3800 ren RPM E E 09 EH HIMN 423 x10 ZON x10 NS CG Ei Iwoprr ESI STOP Basic Screen During a Rundentailed Screen During a Run The values of the 3 basic parameters usually speed temperature and time are quickly introduced after selecting New Appli the stand by display to show the simple programming screen Speed is indicated by a flashing arrowhead gt Use the keypad to write a new speed value if required Move to temperature using AV Use the keypad to write a new temperature value If required use the to change the sign of the value Move to time using AV Use the keypad to write a new time value if required HOLD is achieved by entering a value of O min After making the required modification s either select End Prog to return to the stand by screen ready to run but without storing the values or select Save Appli to store the values first Note If they had been programmed previously RCF could replace speed and integral could replace time in the basic parameter menu Note In either case the new values are held in a buffer memory identi fied by where they remain until the next time End Prog or New Appli is used A Spe
28. 180 13 5 50 107 266 254 blue 11178211 Yellow 6 20 5 7 vac 120 13 5 70 150 266 254 11178212 Red 6 19 10 15vac 114 17 70 150 266 254 11178304 White 6 12 15 conical 72 17 122 266 254 11178306 Coral 6 12 20 72 22 2 75 148 271 259 11178308 Blue 6 5 50 conical 30 30 115 280 268 11178309 Blue 6 4 50 24 35 75 153 283 271 11178311 Turquois 6 1 250 flat 6 63 75 160 266 254 e bottom 11178318 Black 6 1 250 conical 6 61 127 281 269 11178312 Black 6 1 500 flat 6 70 127 171 281 269 bottom 11178203 White 6 1 1000 or 6 282 270 quad bag 11178108 white 6 1 Double bag 6 271 259 Table 1 7 RHK4B ACCESSORIES IN 11174023 BUCKETS AND 11178801 TRAYS CAT N SET OF 2 INSERTS 11179472 CAPACITY 3 sets of 4 inserts N o inserts 6 Tubes per insert 11 Volume ml 50 Table 1 8 RHK4B ACCESSORIES IN 11174023 BUCKETS Set of 2 standard buckets 11174023 Thermo Fisher Scientific CATALOGUE N SET OF 2 TRAYS WITH SPACERS 11178801 Q x HEIGHT min max mm Total tubes 66 31 65 110 CAPACITY 2x15 LKB KR4i Chapter 1 Presentation of product RS485 Specification Communication Type of transmission line differential Maximum number of drivers 32 Maximum number of receivers 31 Maximum cable length 1500 m Maximum data rate 9 6 K octets s Maximum CMV 7 V Driver output 1 5 6 V Driver load 60 omega Note For the physical characteristics relating to RS4
29. 85 communication please refer to the specifications described in the user manual 3 89000821 chapter 3 Mechanical Risks mechanical risks under normal use conditions are as follows and Mechanical Safety KR4i Cause Bucket that ruptures or escapes from rotor Effect Internal explosion due to impact of buckets and rotor on the bowl Design Safeguards Lid interlock system Armored chamber around bowl Highly stable chassis Rupture of rotor or trunnion pin Internal explosion due to impact of buckets and rotor on the bowl Lid interlock system Armored chamber around bowl Highly stable chassis Broken drive shaft or drive system component Internal explosion due to impact of buckets and rotor on the bowl Lid interlock system Armored chamber around bowl Highly stable chassis Rupture or leakage of a sample during centrifugation Imbalance that causes severe vibrations and in the worst case a crash of the rotor and buckets Automatic imbalance shutdown Lid interlock system Armored chamber around bowl Highly stable chassis Insufficient dynamic balancing of samples Automatic imbalance shutdown Imbalance that causes severe vibrations and in the worst case a crash of the rotor and buckets Lid interlock system Armored chamber around bowl Highly stable chassis Loss of seal or rupture of the refrigeration system or one of its components Pressurized
30. D without rotor lid 3800 4 310 267 3 800 4585 284 3800 4310 267 with rotor lid 4 250 5392 267 1 6 KR4i 4 250 5735 284 4 250 5392 267 Thermo Fisher Scientific Chapter 1 Presentation of product Blood Banking 11178155 Set of 6 Blood Banking 11178136 Set of 6 Large Volumes 11178136 Set of 6 11178256 with partition 2 quin or quad bags 11178135 Set of 6 11178135 Set of 6 11178157 with partition 2 quad bags 11178203 1 triple or quad bags 11178195 Set of 6 for 4 11178108 1 double bag buffy coat bags RPM RCF RAD RPM RCF RAD RPM RCF RAD without rotor lid 3800 4536 281 3 800 4585 284 3 800 4585 284 with rotor lid 4 250 5674 281 4 250 5735 284 4 250 5735 284 Table 1 3 RC6 Biochemistry Blood Banking Large Volumes 11178136 Set of 6 11178136 Set of 6 11178136 Set of 6 11178135 Set of 6 11178135 Set of 6 11178135 Set of 6 See detailed table 11178203 1 triple or quad 11178203 1000 ml bags 11178108 1 double bag RPM RCF RAD RPM RCF RAD RPM RCF RAD without rotor lid 3800 4585 284 3 800 4585 284 4300 5623 272 with rotor lid 4 250 5 735 284 4 250 5 735 284 4900 7 301 272 Table 1 4 RHK 4B RIA RIA bucket 11174023 Set of 2 Trays See detailed table RPM RCF RAD 3 300 2 300 189 Thermo Fisher Scientific KR4i 1 7 Chapter 1 Presentation of product Table 1 5 RP6 ROTOR ACCESSORIES IN 11178138 AND 11178140 BUCKETS CAT N C
31. Instruction Manual KR4i Analyze Detect Measure Control SC IE Net fe lec Instruction Manual Jouan KR4i PN 50116933 1 Issued 08 08 Thermo Table of contents Table of contents Thermo Fisher Scientific Table of COMPA Sinai di i POF WONG sierto iii Accident Prevention aes earn etd niae ccr ku dudo cR Ek 2d Rorate eor xi Ea iv PSG AUT OVS n V Chapter 1 Presentation of product 1 1 DGSCIOUON P 1 1 Relative Centrifugal Force ss 1 1 Centrifugation TOmHnmuldG ce cator unttte rn Rem oput a 1 1 Drive System oi ita 1 2 Refrigeration System 1 2 Dimensions and Weight 1 3 Electrical Specifications 1 3 F artormisfib eoi t abet etme eae 1 3 ACOESSONES ui eriperet cene eo PR SE eee ud 1 5 RS485 Specification Communication 1 10 Mechanical Risks and Mechanical Safety 1 10 Internal and External Markings of Centrifuge 1 11 RESPONSIDIMY MON 1 11 Chapter 2 Shipment Transportation 1 Environmental Conditions for Transport 2 1 Lifting and Transport iaciunt rtt teret tette 2 1 Chapter3 Storage aoe ee MR a iEn 3 1 Chapter4 Installation uisi inset ncs creda aad g i E pEE0Y Md 0408 saca siena as 4 1 Environmental Conditions sseessssssss 4 1 Electrical
32. Manual Lid Opening 4 8 KR4i Thermo Fisher Scientific Chapter 4 Installation Inspection Rotor Installation Windshielded Rotors RP4 RP6 RC6 With Lid Thermo Fisher Scientific Before installation the rotor should be thoroughly inspected for cor rosion and cleanliness Chemical and stress corrosion will eventually lead to disruption of the rotor with potential severe damage to the centrifuge Particles stuck inside the pockets can cause breakage of tubes and lead to major imbalance and or loss of sample and contamination Check that the central hole of the rotor and the drive spindle are clean and undamaged Remove any particles with a soft cloth Refer to chapters 5 9 and 5 10 Rotor installation must only be undertaken by qualified and authorized personnel trained by Thermo Fisher Scientific Never lift a rotor by its windshield The rotors and buckets used with the Jouan KR4i are very heavy When handling these accessories take appropriate precautions to prevent back or other skeleto muscular injury Ensure that the rotor is attached properly to the drive shaft and that the windshield lid is attached properly to the rotor Any failure to do so may prevent the rotor buckets from operating properly The lid delivered separately from the rotor The rotor packing contains arotor grease for the trunnions and shaft ausers manual The lid packing contains e arotorlid e a lid locking device
33. Nitrate Ferric Sulphate Ficoll Paque Fluorhydric Acid 10 Fluorhydric Acid 50 Formaldehyde 20 Formaldehyde 40 Formaldehyde 50 Formic Acid 100 Glutaraldehyde Hydrochloric Acid 5 Hydrochloric Acid 37 Hydrochloric Acid 50 Hydrochloric Acid conc Hydrofluoric Acid 10 Hydrofluoric Acid 50 Hydrogen Peroxide 3 Hydrogen Peroxide 100 lodine Crystals Isobutyl Alcohol Isopropyl Alcohol Isopropylbenzene Lactic Acid 20 Lactic Acid 100 Fluorine Freon TF Fuel Oil Glucose Glycerine Glycerol Heptane Hexane Hydrazine Kerosene 5 30 Using the Product Chapter 5 XOJ9A V UOUA uiniuelr IezjeL uoper 99 S ssojureis SUOJNS JOd euejAdoud AJod euoej uieAJod ayeuoqiedAjod 09dd 1euiojeAjoq uoJ N AION Jeu M uujeg 9 EJIIN 9SO N 9D 9 eJ1 mq ojejooe eso nijo2 N eung uiniuiunj y Jenae 5 31 O O O KR4i Mt M Mt Mt Mt Mt Mt Mt O O O S O O O O O U M U M S M S S O S St O X M U M M St S S S Magnesium Hydroxide Methyl Isobutyl Ketone Methyl Isopropyl Ketone Oxygenated Water 20 Oxygenated Water 50 Oxygenated Water 90 Lead Acetate aq Lemon Essence Lime conc Magnesium Chloride Magnesium Nitrate Magnesium Sulphate Manganese Salts Mercury Methanol 100 Methyl Acetate Methyl Alcohol 100 Methyl
34. ONTAMINATION DATE DECONTAMINATION METHOD S DECONTAMINATION CERTIFIER S SIGNATURE DATE INSTRUCTIONS When an instrument that has been used with radioactive patho genic or otherwise hazardous materials requires servicing by Thermo Fisher Scientific personnel either at the customer s labo ratory or at Thermo Fisher Scientific facilities the following proce dure must be complied with to insure safety of our personnel 1 The instrument or part to be serviced shall be cleaned of all blood and other encrusted material and decontamina ted prior to servicing by our representative No radioacti vity shall be detectable by survey equipment 2 A Decontamination Information Certificate shall be com pleted and attached to the instrument or part If an instrument or part to be serviced does not have a Decontam ination Information Certificate attached to it and in our opinion presents a potential radioactive or biological hazard our represen tative will not service the equipment until proper decontamination and certification has been completed If an instrument is received at our Service facilities and in our opin ion poses a radioactive or biological hazard the sender will be contacted for instructions as to disposition of the equipment Dis position costs will be borne by the sender Decontamination Information Certificates are included with these Operation Instructions Additional certificates are available fr
35. R4i 5 1 Chapter 5 Using the Product Liner without partition Filter bag liner Balancing The Load KR4i Never centrifuge an empty liner because there is a risk of deformation Load the two blood bags into the liner simultaneously Hit the base of the liner on the bench top to make the bags drop well down and ensure that they touch the bottom of the liner At the end of the centrifugation unload the two bags simulta neously in order to avoid resuspension of the products Never centrifuge such a liner loaded with only one bag This cre ates the risk of a bad separation of products Never centrifuge an empty liner because there is a risk of deformation Place bags in each side of the liner leaving the filters outside Hit the base of the liner on the bench top to make the bags drop well down and ensure that they touch the bottom of the liner Insert the filters one after the other by sliding them between the bags until they sit on the support mounted on the partition Insert the tubing such that it cannot escape from the liner and bucket dur ing centrifugation Never centrifuge such a liner loaded with only one bag This creates the risk of a bad separation of products and could provoke a deformation or even a rupture of the central wall Never centrifuge an empty liner because there is a risk of deformation When installing tubes into each bucket insert be certain that the samples are distributed symmetrically S
36. Rotors may be decontaminated by a mixture of equal volumes of a Distilled water b SDS diluted to 10 c Ethanol diluted to 70 Thermo Fisher Scientific Chapter 5 Using the Product Preventative Maintenance Storage of Rotors Thermo Fisher Scientific The rotor should then be rinsed with ethanol followed by distilled water and then dried completely Thermo Fisher Scientific makes no claims as to the effectiveness of proprietary brands of decon taminating solutions In the interests of prolonging metallic accessory life it is advisable to smear the pockets with a silicone based grease such as SILI CONE GREASE 500 from KF or to use a LANOLIN based grease Thermo Fisher Scientific is able to supply cans of Anti Corrosion Spray catalogue N 11175399 whose propellant drives out water based moisture and leaves a protective lanolin film on the surface It is strongly recommended that rotors not in use should be stored upside down on a non metallic grid or other support which allows free movement of air Any moisture contained in the pockets will then be eliminated by gravity and evaporation If the rotors are to be stored for a long period then we advise that they are dried per fectly and greased with silicone grease High speed rotors are frequently preconditioned by storing in a cold room or refrigerator This tends to advance the onset of corrosion We recommend therefore that such rotors are stored dismantled sea
37. Suggested Template for Annual Centrifuge Safety Inspection 6 2 Rotor Removal std euo oed LE eut 6 4 LID Opening Manual LID Unlocking 6 5 Chapter7 Disposal of Product esses 7 1 Annex A Warranty conditions esee A 1 AnnexB Declaration of conformity eec B 1 KR4i Thermo Fisher Scientific Thermo Fisher Scientific Forword Foreword Before starting to use the centrifuge read through this manual carefully and follow the instructions The information contained in this instruction manual is the property of Thermo Fisher Scientific it is forbidden to copy or pass on this information without explicit approval Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the seller s war ranty KR4i iii Foreword KR4i Accident Prevention Prerequisite for the safe operation of the KR4i is a work environ ment in compliance with the following standards directives and trade association safety regulations and proper instruction of the user EEC Directive 89 391 EEC on Measures for Improving the Safety and Health Protection of the Worker on the Job Accident Prevention amp Insurance Association Regulation A1 Principles of Prevention Accident Prevention amp Insurance Association Regulation A3 Electrical Systems and Equipm
38. Tel 4331 69 18 77 77 33 2 28 03 20 00 NETHERLANDS Tel 431765714440 UNITED KINGDOM or IRELAND Tel 44 87 06 09 92 03 UNITED STATES of AMERICA Tel 1 800 522 77 46 1 866 984 37 66 In Other Countries Contact your local Thermo Fisher Scientific products distributor or agent INDIA JAPAN Tel 91 22 55 42 94 94 Tel 81 454 53 92 20 OTHER ASIA PACIFIC COUNTRIES Tel 852 28 85 46 13 BELGIUM FINLAND Tel 32 24 82 30 30 Tel 35 89 32 91 00 GERMANY ITALY Tel 44961 8490 69 40 Tel 39 02 95 05 91 49 61 03 40 81 012 SPAIN or PORTUGAL SWITZERLAND Tel 434932233154 Tel 41 1 454 12 12 Other EUROPE RUSSIA CIS MIDDLE EAST or Tel 70952251115 AFRICA Tel 4961 8490 6940 38 2 28 08 20 00 CANADA or LATIN AMERICA Tel 1 800 522 77 46 1 866 984 37 66 Dans les autres pays Contactez In anderen L ndern Wenden Sie votre concessionaire local des sich an Ihren rtlichen Thermo produits Thermo Fisher Scientific Fisher Scientific Vertreter ou leur representant E mail Technical Service Representative for Thermo Fisher Scientific brand products at techsupport led asheville thermofisher com Visit our web site at http www thermo com
39. able regulations The Jouan KRdi centrifuge is delivered without an electrical plug this must be connected by a qualified engineer trained by Thermo Fisher Scientific according to the following con ductor colour code Blue neutral brown phase yellow green ground Depending on the equipment part number the electrical supply should be 230 V 10 50 Hz 25 A max 208 230 V 60 Hz 25 A max Remember that in order to respect the electrical safety standards related to protection of operators against indirect contact the sup ply of power to the instrument must be via a power socket fitted with a protection device ensuring automatic cut off in the case of an insulation fault Thermo Fisher Scientific Chapter 4 Installation Positioning The 2 front feet Thermo Fisher Scientific A supply fitted with a circuit breaker of the correct rating complies with this requirement It is also recommended that different machines be connected to a circuit breaker that is situated outside the room It must be immediately possible to identify the plug of the machine which must also be easy to reach by the user It must be immediately possible to identify the plug of the machine which must also be easy to reach by the user Concerning the electrical supply to the centrifuge there must be a circuit breaker outside the room where the centrifuge is installed that can be used to interrupt the power supply to the centrifuge in case o
40. an Allen key e a special screw for rotor fastening an instruction sheet for assembling the lid locking device Check cleanliness of the motor drive shaft and rotor hub Apply a light film of grease to the motor shaft KR4i 4 9 Chapter 4 Installation Winshielded Rotors RP4 RC6 RP6 Without Lid Unshielded Rotor RHK4B All Rotors Winshielded Rotors 4 10 RP4 RP6 With LID RHK4B KR4i Note the collar position on the shaft and on the rotor two indi cators on the rotor label sticker allow localization of the collar groove Pick up the rotor by two opposite arms and place it on the shaft ensuring shaft and hub alignment Position the rotor gently despite its weight The rotor slides freely onto the drive shaft Apply a light film of grease to the tip of the trunnions e Adjust the lid locking device as mentioned in the instructions Position the rotor lid on rotor verify that the lid locking device locks on to the rotor The packing contains e arotor e grease for trunnions and shaft e aninstruction sheet Check cleanliness of the motor drive shaft and rotor hub Apply a light film of grease to the motor shaft Note collar position on the shaft and the groove on the hub Pick up the rotor by two opposite arms and place it on the drive shaft ensuring shaft and hub alignment Position the rotor gently despite its weight The rotor slides freely onto the drive shaft Position th
41. between two adjacent machines to allow subsequent placement of a vessel to collect condensates If the centrifuge is installed next to other equipment or next to a walkway leave a space of 65 cm between equipment and 85 cm between the equipment and the walkway Centrifuges should be placed preferably in a room specifically intended for their use and therefore containing no other apparatus Walls and partitions that are within a distance of 1 metre should be strong enough to stop the centrifuge should there be a major acci dent mobile partitions single width chipboard and plasterboard partitions without reinforcement are not strong enough The floor must be completely flat and strong enough to support the weight 346 kg of the machine without creating or maintaining any vibrations Vibrations may be caused by variations during centrifu gation See also following chapter for positioning the damping feet There are no set minimum specifications on the type of floor required as there are many technical factors to be taken into account such as KR4i 4 1 Chapter 4 Installation KR4i Composition of the different layers of the floor How the floor is joined to the wall e Structures supporting the floor Positions and weights of loads already on the floor Loads already existing on support structures The machines must be positioned so that they can be moved easily to enable regular access for maintenance This c
42. cceleration KR4i If necessary enter the Operator Code via the keyboard the ENTER or ESC to abort USE KEYPAD TO SET USER IDENTIFICATION EHTER TO LTDA E ESC TO ABANDON CENTRIFUGE ID After the identification of the liners by the PC identify the liners to be centrifuged by the Jouan KR4i using the bar code reader on the centrifuge Read the ID of the first support then press ENTER Continue up to the last support a maximum of 6 for CQCS V3 0 USE BARCODE READER OR KEYPA MEME don LINERS TO BE CENTRIFUGED EHTER TO VALIDATE WHEN USING KE ESCTO ABANDON START TO CENTRIFUGE MEI E 2 rm 3 E 4 LINER 5 LINER 6 Esc sc 7 Select START to start the run START kO For instructions on operating the CQCS application please refer to the CQCS user manual cat n 89000821 The Jouan KR4i centrifuge allows a choice of 10 different accelera tion rates thus permitting variation of the time for the centrifuge to attain the desired speed If the centrifuge is used for blood transfusion purposes the choice of an intermediate acceleration rate allows optimal blood bag posi tioning in the buckets thus limiting the risk of bursting and forma tion of wrinkles Moreover to carry out selective separations such as separation of platelets it is possible to improve the results by adjusting the acceleration rate Thermo Fisher Scientific Chapter 5 Using the Product The table below shows
43. ce and highest safety with the instrument and its accessories The information given below is succinct Please refer to the manual specific to the use of centrifuges in blood transfusion centres Cat N 89001172 Liners are consumable items which must be replaced after several hundred runs according to their condition Liners with partition must be loaded symmetrically about the parti tion The load on one side must never exceed the load on the other side by more than 100 g under risk of permanent damage to the liner Bags filled with water can be added if necessary to balance the load Supple liners are available for existing users New users are advised to use the semi rigid liners which are easier to manipulate Place a blood bag in each compartment The two bags must have approximately the same mass such that the centre of gravity of the filled liner is in line with the partition This ensures a horizontal atti tude during rotation which guarantees correct centrifugation in the bags When the number of bags to be centrifuged is insufficient to fill all compartments complete the load with dummy bags filled with water Hit the base of the liner on the bench top to make the bags drop well down and ensure that they touch the bottom of the liner Never centrifuge such a liner loaded with only one bag This cre ates the risk of a bad separation of products and could provoke a deformation or even a rupture of the central wall K
44. ckets polypr 29min 18min 12min 9 min 7 min 5 min 4 min 4 min 3 min 3 min with lid liner without partition 30sec 50sec 20sec 10sec Osec 20sec 30sec Osec 40sec 20sec 4250 rpm triple bags d 1 0 RC6 N6C buckets 19min 12min 8 min 6 min 4 min 3 min 3 min 2 min 2 min 2 min without lid adaptor for bottle 40sec 10sec 10sec 10sec 30sec 20sec Osec 40sec 20sec 10sec 4300 rpm 1000 ml bottle d 1 0 RC6 N6C buckets 23min 15min 10min 7 min 5 min 4 min 3 min 3 min 3 min 3 min with lid adaptor for bottle 50 sec 0 sec Osec 20sec 70sec 30sec 50sec 30sec 10sec 0 sec 4900 rpm 1000 ml bottle d 1 0 RHK4B NHK4B buckets 14 min 9 min 6 min 4 min 3 min 2 min 2 min 1 min 1 min 1 min 3300 rpm stainless steel 30sec 20sec Osec 20sec 10sec 20sec Osec 40sec 30sec 30sec trays LKB trays 3 8 kg per liner The above values are given for the indicated load and nominal operating voltage These values change if the voltage and or the load are different Integ ration setting centrifugation parameters consists of determining the time Centrifugal acceleration and during which the sample is submitted to the selected centrifugal acceleration In practical terms centrifugal acceleration is set in terms of motor duration rotation speed rpm A given rotation speed corresponds to centrif ugal acceleration which is directly proportional to the centrifugation radius in keeping with the following equation 2 Number of g cada R N KR4i Acceleration x C
45. destroy micro organisms Rotor lids must be disassem bled from rotor bodies O rings autoclaved separately should be replaced when deformed ANY PART WHICH HAS BEEN SUBJECTED TO TEMPERA TURES ABOVE 130 C MUST BE DISCARDED Hypochlorite bleach used at 0 1 concentration with 10 minutes immersion is effective against bacteria spores and viruses but as an oxidizing agent is corrosive to metal alloys and must be thor oughly rinsed off and dried It should never be used if there is sur face damage to the rotor Note that the black colour on the surface of the rotor will be gradually washed out if the rotor is regularly autoclaved or bleached This does not necessarily denote a degra dation in the anodization Formalin 37 formaldehyde in water in contact for 10 minutes has a similar effect to chlorine bleaches Rotors should be thor oughly rinsed under running water for 5 minutes to remove all traces of formalin then dried completely NOTE FORMALDEHYDE IS TOXIC Gluteraldehyde 2 sold under many brand names such as Cidex and Glutarex requires total immersion for 10 minutes to ensure sterility Thorough rinsing and drying is essential to protect users CAUTION GLUTERALDEHYDE BUILDS UP TO A TOXIC LEVEL IN THE FATTY TISSUES OF THE BODY Phenols are very corrosive and should never be used Radioactive decontamination We recommend that all radioactive contamination be referred to your Radioactivity Safety Officer
46. e 5 32 Using the Product Chapter 5 XOJ9A V UOUA uiniuelr IezjeL uoper 99 S ssojureis auojinsAjod euejAdoud AJod aus Ayl9 Al0d ayeuoqiedAjod 09dd 1euiojeAjod uoJ N AION Jeu M uujeg 9 EJIIN 9SO N 9D 9 eJ1 mq ojejooe eso nijo2 N eung uiniuiunj y lenaren 5 33 O KR4i O Sd Sd Sd S O Sd Sd Sd S S S M M M M U O U U O O M M S S U U U M S M U U U S M O Sodium Carbonate 2 Sodium Chloride 10 Sodium Chloride Sat d Sodium Hydroxide gt 1 Sulphuric Acid 10 Sulphuric Acid 20 Sulphuric Acid 50 Sulphuric Acid conc Sulphuric Anhydride Sodium Hypochlorite 5 dry or moist Saccharose Salycylic Acid Sat Serum Silver Acetate Silver Nitrate Sodium Acetate Sodium Bisulphate Sodium Borate Sodium Bromide Sodium Hydroxide 10 Sodium Hydroxide conc Sodium lodide Sodium Nitrate Sodium Sulfate Sodium Sulphide Stearic Acid Tetrachloroethane Tetrachlorethylene Tannic Acid Tartaric Acid Tetrahydrofuran Thionyl Chloride Toluene Trichlorethylene Thermo Fisher Scientific Chapter 5 Using the Product 2 S pes O 5 O a a 2 2 A DL o o gt E sz 325 2 o E ses t 6 9 65 8 T 9 o 2 gt 2 9 o E
47. e washer and the bolt and screw clockwise with the wrench supplied Never tighten the rotor bolt without the washer Apply a light film of grease to the tip of the trunnions Ensure that the accessories are correctly fitted and balanced e Check that the rotor is fully locked onto the drive shaft e Check that the rotor lid fittings are screwed on tightly Check that there is no water nor other liquid in the base of the windshield e Check that all of the buckets are in place and that they swing freely e Check that there are no empty transfusion liners in the buckets Thermo Fisher Scientific Chapter 4 Installation Rotors P40 P60 C60 HK4B Power Off Operation Thermo Fisher Scientific Use only rotors and buckets specifically designed for the Jouan KR4i Please contact us for information on P40 P60 C60 and HK4B rotors The full power off of the Jouan KR4i centrifuge is obtained by dis connection of the power cord from the mains supply Immediately after disconnection and for a duration of not longer than a minute voltage remains on centrifuge line cord connectors Do not touch for at least a minute KR4i 4 11 Chapter 4 Installation 4 12 KR4i Thermo Fisher Scientific Handling of Transfusion Accessories Liner with partition Thermo Fisher Scientific Chapter 5 Using the Product It is essential to understand the precautions risks and limitations of use to achieve the best performan
48. ed related RCF acceleration braking rotor radius To program any of the speed related parameters select DETAILS from the simple programming screen when the flashing cursor gt indicates SPEED A screen appears with the speed related param eters and the flashing arrowhead indicates RCF A value can be input via the keypad so long as a rotor has already been selected No selection of rotor makes it impossible for the software to control by g force since this is directly linear with the Thermo Fisher Scientific Chapter 5 Using the Product Thermo Fisher Scientific radius of the base of the sample tube from the centre of the drive axis To choose a rotor select ROTOR and use V to move the cursor down the list of rotors to that which will be used If an adaptor will be used in the rotor select RADIUS and use the amp keys to choose the effective radius and select ENTER During operation with amp the dynamic radius arrow moves and the related RCF is changed in real time If the user wishes to modify the acceleration and or braking rates he selects ACC or BRK and uses the amp keys to choose the rate s indicated on the dynamic graphic screen Select ENTER to return to the speed related parameter screen After selection of ROTOR and maybe RADIUS ACC BRK the operator has the option to select MAX Without requiring any knowledge on the part of the operator selection of MAX causes the instrument to program the
49. ed to proximity to other stored goods Away from all inflammable or explosive goods Away from all corrosives or strong solvents that may be spilled or spread and that may for any reason come into contact with the packaging Note The machine must be handled using all necessary precautions using a forklift or pallet jack by trained and authorized personnel KR4i 3 1 Chapter 3 Storage 3 2 KR4i Thermo Fisher Scientific Environmental Conditions Thermo Fisher Scientific Chapter 4 Installation Note The Jouan KR4i centrifuge must be installed by a Thermo Fisher Scientific approved technical service provider The user must four nish the Thermo Fisher Scientific technical service provider with a source of electricity and when applicable with a source of water that is in strict conformity with local codes and regulations After installation the integrity of the source s should be periodically veri fied by the user This instrument is designed to operate safely under the following conditions Indoor use Temperature 5 to 40 C Maximum relative humidity of 80 for temperatures up to 22 C Maximum altitude 2000 m Maximum performance is assured across the following ambient temperature range 15 C to 25 C Place the centrifuge and its accessories in a clean non corrosive environment to avoid damage Install the unit leaving at least 30 cm 12 in clearance between its back and any obstruction and
50. eekly Cleaning Thermo Fisher Scientific Chapter 6 Maintenance It must be immediately possible to identify the plug of the machine which must also be easy to reach by the user Users must not use cleaning or decontamination methods different from those recommended without first having verified that these methods do not risk damaging the instrument or its accessories and obtaining prior authorization from the manufacturer Users must use the adequate protection needed for the cleaning product used in each case Please note that no maintenance performed by the user requires the user to open the panels to expose the internal components of the centrifuge All service other than routine service that can be performed by the user must be performed by qualified technicians trained by Thermo Fisher Scientific Daily cleaning is not required except in the event of glass break age or a large amount of spillage in the rotor chamber or fairing If you wish to clean the control panel Turn off the centrifuge e Clean with a moist sponge and a soap product and rinse with a moist sponge Dry with a lint free cloth To disinfect if required use 70 alcohol a Inthe event of spillage between the chamber and the windshield clean the rotor chamber with a detergent and water only First apply a mild water detergent solution to a clean rag Then use the rag to remove all traces of the spill from the bowl of the centrifuge and from the
51. elect CONFIG from the stand by screen A Language programming Having selected CONFIG the screen highlights LANGUAGE SELECTION then select ENTER Select 1 for ENGLISH or 2 for FRENCH The stand by screen will be displayed in the selected language B Selection of refrigeration stand by Having selected CONFIG use V to highlight STAND BY REFRIGERATION ON OFF then select ENTER Choose 1 for active refrigeration during stand by and 2 for refriger ation OFF during stand by C CQCS option see following page D Timer configuration Select 1 to program hours minutes Select 2 to program min utes seconds E Service A special code reserve Your centrifuge can be equipped with the CQCS Centrifugation Quality Control System This system allows the traceability for centrifuges the products and the operators and follows the historical use of each instrument It is thus also considered as a veritable maintenance tool With versions 3 0 and later it is possible to transfer the centrifuga tion run data from up to 32 centrifuges to a PC CQCS ensures traceability of samples by a system of twin bar code readers This system includes two principal stages 1sistage Association Support Sample This stage concerns the identification of supports liners and sam ples blood bags using a bar code reader connected to the PC see the BB CQCS manual 2 stage Association Support Centrifuge This stage concerns the identificat
52. ende bedrijven in alle EU Lidstaten overeengekomen om dit apparaat te recyclen of veilig te verwijderen Gegevens over de afvalverwerkende bedrijven in Duitsland evenals informatie over producten van Thermo Fisher Scientific die onder de RoHS richt lijn vallen engl Restriction of the use of certain hazardous sub stances in electrical and electronic equipment Beperking van het gebruik van bepaalde gevaarlijke stoffen in elektrische en elektroni sche apparaten vindt u op het Internet terug onder www thermo com WEEERoHS Thermo Fisher Scientific Portugal Thermo Fisher Scientific Declaration of conformity Conformidade com a Diretiva Europ ia REEE Este produto est sujeito s determina es da Diretiva Europ ia de Res duos de Equipamentos El tricos e Eletr nicos Diretiva REEE 2002 96 Isto caracterizado pelo s mbolo ao lado Thermo Fisher Scientific A fechou um acordo com todas as empresas de reciclagem e de elimina o de res duos em todos os Estados Membros da UE para que o produto seja reciclado ou eliminado Quaisquer informa es sobre as empresas de reciclagem e de elimina o de res duos na Alemanha bem como sobre produtos da Thermo Fisher Scientific que est o sujeitos Norma RoHS em ingl s Restriction of the use of certain hazardous substances in electrical and electronic equipment Restri o do Uso de Subst ncias Nocivas nos equi pamentos el tricos e eletr nicos podem ser encontradas
53. ent BGR 500 Operation of Equipment Chapter 2 11 Machines of Chemical Processing Technology Part 3 Machine Directive 98 37 EC Low Voltage Directive 72 23 EC 93 68 EC EMC Directive 89 336 EG 92 31 EG 93 68 EG EN 292 EN 61326 EN 61000 3 2 A14 2000 06 EN 55011 B Radio Interference Suppression EN 61000 6 2 Safety Standard EN 601010 1 EN 61010 2 020 IEC 1010 2 020 This centrifuge fulfils the requirements of the international safety standard IEC 1010 2 020 for laboratory centrifuges The safety regulations contain the following basic recommenda tions Maintain a radius of at least 30 cm around the centrifuge Implementation of special measures which ensure that no one can approach the centrifuge for longer than absolute necessary while it is running installation of an easy to use and always accessible emer gency stop switch that cuts off the power supply in an emer gency Such a switch must be installed at a certain distance to the centrifuge preferably in another room Thermo Fisher Scientific Thermo Fisher Scientific Forword Precautions In order to ensure safe operation of the KR4i the following general safety regulations must be followed The centrifuge should be operated by trained personnel only The centrifuge is to be used for its intended purpose only Proper use on page vi Do not move the centrifuge while it is running Do not use rotors which show any sig
54. entrifugation radius mm Speed in thousands of rmp For example a given separation may be described as obtainable by centrifuging the sample in a given machine which determines the accessory thus the centrifugation radius for 10 minutes dura tion at 4000 x g R C F Theoretically if the centrifuge instantly attains the set point speed and brakes instantly and if the requested speed attained is con stant reproducibility of results is guaranteed from one centrifuga tion to the next Thermo Fisher Scientific Chapter 5 Using the Product The centrifugation curve is as follows E c 2 pan 2 T o o 6 a 2 3 E E E o 10 Duration min Figure 5 2 Ideal centrifugation integral curve It is thus obviously equivalent to centrifuge a sample at 4000 g dur ing 10 minutes as to centrifuge it at 2000 g for 20 minutes or 8000 g for 5 minutes In other words centrifugations yield the same results when the product DURATION X CENTRIFUGAL ACCELERATION remains constant This product corresponds to the area of the curve Centrifugal acceleration g 0 5 10 15 20 25 Duration min Figure 5 3 Equivalent centrifugation integral curves Thermo Fisher Scientific KR4i 5 17 Chapter 5 Using the Product 5 18 Integral of the curve Using the Integrator KR4i The integral of the curve is the value of its area The area repre sents the total amount of separating force applied to the sa
55. entrifuge should only be installed in a work area that is lighted to the requirements of the local labor code The IEC 1010 2 020 standard relative to laboratory centrifuges rec ommends the user To mark out a clearance envelope 30 cm around the centrifuge or establish special procedures for the non entry of all persons or all dangerous materials into this space during the operation of the centrifuge Thermo Fisher Scientific Chapter 4 Installation Thermo Fisher Scientific KR4i 4 3 Chapter 4 Installation Electrical Requirement KR4i Read the following instructions carefully before installation During manipulation never put your hand underneath the machine Remove staples with a screwdriver Remove both anchor bolts A using the 19 mm spanner wrench supplied in the tool box Remove the front anchor bolt B Using the prop C as a lever Remove the wooden beam and portable ramp D by lifting the machine at the back Remove the wooden beam and portable ramp E by lift ing the machine at the right hand side towards the front Ensure that the ramps F are perpendicular to the pallet and in line with the wheels 2 people are needed to unload the machine 1 person at the front of the centrifuge to guide and brake the machine as it comes down from the pallet a second person behind to guide the machine onto the ramps The electrical supply circuit must conform to currently applic
56. eration level R C F Upon attainment of that value the centrifuge braking phase is started automatically The centrifuge calculator always provides values of the centrifuga tion integral over time Thus the optimization of centrifugation and determination of integrals to be entered is considerably simplified During a series of runs using the timer as usual at the run end select MODIFY AV AV time DETAILS The instrument will display the value of the integral measured automatically during the run Note this value together with the sample identities When the samples from the various runs have been analysed note those which had the best results and look up the recorded integral value Thermo Fisher Scientific Chapter 5 Using the Product Refrigeration Thermo Fisher Scientific System Reprogram the run memory by choosing MODIFY AV AV time DETAILS then write the value of the integral then save the program by choosing ENTER SAVE PROG as usual Now the centrifuge will automatically make small adjustments to the time slighily longer for high loads poor balancing etc In this way the identical total separating force will be supplied to all samples The only differences in results will be due to the samples not to the centrifugation The use of the integrator is strongly recommended for blood platelet separation The Jouan KR4i is equipped with a refrigeration system which allows the rotor chamber temperature to
57. es a protec tive layer on the surface of the rotor Acidic and alkaline solutions sustaining their pH level will create problems of corrosion in aluminium rotors Chlorides present in salts or even in skin contact with the rotor are among the most aggressive and harmful substances commonly found in the laboratory The chemical products which are the origin of this corrosion do not necessarily originate from broken tubes for example they could come from Chemical vapors present in the laboratory which are dissolved in the residual humidity in condensed water refrigerated cen trifuges present at the base of the rotor pockets Corrosive liquids originating from overfilled uncapped tubes the liquid overflowing during centrifugation Inserts adaptors racks bottles whose exterior has been soiled by a chemical product or poorly rinsed after decontamination with bleach for example Note If the products are very corrosive simply rinsing is insufficient Residual traces dissolve little by little in the humidity present in the bottom of the pocket in the condensed water such as occurs when putting an ambient temperature insert in a cold rotor Beware of the presence of solid particles beneath tubes inserts racks or adaptors These particles are crushed by the centrifugal force and penetrate the protective anodised layer of buckets and rotors thus creating easy pathways for corrosion Stress Corrosion This term re
58. f emergency The IEC 1010 2 020 standard relative to laboratory centrifuges rec ommends the user To provide an emergency stop switch enabling the mains power to be cut in the case of a malfunction This switch should be placed at a safe distance from the centrifuge preferably in a different room from that in which the centrifuge is situated Put the damping foot 4 under the screw of the foot 3 Clean the floor beneath the feet of the machine with a clean dry cloth or paper hand towel Remove the protective film from the adhesive face of the pad Centre the pad in line with the foot and stick it to the floor Press firmly on the whole surface of the pad to ensure a good adherence Using the tool 5 set the foot screw in position and turn the nut 2 until the castors are raised 0 5 to 1 cm above the ground Then lock the screw by tightening the nut 1 KR4i 4 5 Chapter 4 Installation 5 The rear foot For centrifuges equipped with water cooled condensers cat nos 11178161 and 11178163 4 6 KR4i Anti skid pad Figure 4 1 Positioning the Jouan KR4i Using the 17 mm box spanner 6 proceed as for the front feet Set the centrifuge straight on its feet using the spirit level pro vided with the machine Water supply specifications Pressure 2 to 4 bars Flow 2 250 liter min Temperature between 12 C and 20 C Proceed as follows Positions the machine on it
59. f required to do so refer to local or national transportation stan dards The machine is transported in an integrated packaging system of carton and protection fixed to a wooden pallet Required environmental conditions for transport are as follows Temperature 10 C lt lt 50 C Humidity 20 lt RH lt 90 Light no particular recommendation machine is enclosed in opaque packaging Protection from elements the packaging may not be exposed to precipitation Restraints may be necessary to prevent lateral movement of the package Stacking is strictly prohibited Due to the weight of the machine all lifting and transporting must be carried out using proper handling equipment e g fork lift trol ley that complies with current regulations and by people having undergone the necessary training Note The machine must be supported from underneath If it has to be transported without its pallet for example on a staircase profes sional handling assistance is required KR4i 2 1 Chapter 2 2 2 KR4i Thermo Fisher Scientific Thermo Fisher Scientific Chapter 3 Storage Whatever the duration of storage the following conditions apply Environmental conditions e Temperature 10 C lt lt 50 C Humidity 20 lt RH lt 90 Light no particular recommendation machine is enclosed in opaque packaging Storage in a clean area that is not polluted by industrial activity Conditions relat
60. ge selection DETAILS Gives access to those parameters associated with the indicated parameter DISPLAY APPLI Displays all preselected values of the program whose basic parameters are on screen END MODIF Returns to the original run screen after temporary values have been entered END PROG Returns to the stand by screen keeping currently selected values in volatile memory ready for use but not in permanent memory for later recall ENTER Returns to a previous screen memorizing all changes in parameter values ESC Returns to a previous screen without memorizing any changes in parameter values EXPAND DISPLAY Switches the display from the basic run screen to the detailed run screen which displays all parameters HOLD Selects continuous operation for the time parameter value Thermo Fisher Scientific Chapter 5 Using the Product Thermo Fisher Scientific KR4i MAX Directly chooses the highest possible speed or g force taking into account the selected rotor and radius cannot operate without prior rotor selection MODIF amp SAVE Enables parameter values to be changed and saved if the current application has not been already saved MODIF Y Enables parameter values to be changed Values changed during a run other than PRE COOL will not be memorized MORE INFO Provides a more detailed explanation of the current parameter e g definition of the
61. he time integral screen If this appli cation has just been used VIDEOset will indicate the recently totalled integral value Selection of ENTER will return the user to the simple programming screen but with control by integral instead of time ensuring the highest possible run to run reproducibility Note This will only be possible if a rotor has already been selected the integral value being linked to the g force which is calculated from the centrifugation radius of the rotor Alternatively use AV to indicate time and enter a value or press HOLD for operation with no time limit Selection of ENTER makes a return to the simple programming screen To use the modified parameter changes without permanently stor ing them select End Prog During a run controlled by the integrator the preset value for time will indicate REPROducible the integral value will count down and the timer will count up the corresponding time From the simple programming screen select Save Appli if the flashing curser gt is on temperature it will be necessary to select AV to make Save Appli reappear The application save screen appears proposing the next available empty memory If required use X and gt to review other memories whose application pro gram parameter values are indicated at the right hand side of the screen Select ENTER to save the application in the indicated file The user is offered the opportunity to enter an individual pas
62. he user tries to manually stop the rotor Any object falls inside the centrifugation chamber If the machine has stopped because of a mains power cut the rotor could still be rotating Wait 10 minutes before opening the lid In spite of the absence of noise the rotor could still be rotating when you need to open the lid manually KR4i 4 7 Chapter 4 Installation Manual lid unlocking must only be done by someone informed of the danger and of the necessary precautions to be undertaken Even in the case of a mains power cut always set the mains switch to the OFF position before carrying out this operation e Insert the unlocking device into the hole on the top right of the instrument by piercing the protection sticker While pressing down on the unlocking device pull the lid lever forward The lid opens upwards automatically The protection sticker must be replaced after it has been pierced cat n 85241911 230 V 50 Hz cat n 85241912 208 V 60 Hz It is recommended that manual lid opening operation be performed only by specially trained individuals who are authorized by the Lab Manager The person responsible for the laboratory whenever the centrifuge is being used should strictly control the use of the man ual lid unlocking tool Only use the manual lid opening proceudre in case of power failure or failure of the electrical lid lock system and then only with the main switch in the OFF position Figure 4 3
63. ide of the screen Now either select ESC to return to the standby screen or ENTER to save the modified application in the indicated file mem ory The user can decide whether or not to password protect the new protocol which can now be stored in this memory A From the stand by screen With an application program on the stand by screen select Modif New Now alter program parameters exactly as in programming a New Appli To SAVE select Save Appli from the simple programming screen and continue as under 5 4 6 A new memory will be proposed To save in the original memory erasing original values use lt or gt to move the cursor and enter the password if requested B During a run From the basic or detailed run display select MODIFY The three main control parameters speed or RCF temperature time or inte gral will be displayed together with the current set values Use AV to move the cursor gt to the parameter to be modified and enter the value via the keypad Select End Modif to return to the original run screen Values modi fied during a run cannot be stored such a randomly modified run would be impossible to reproduce but remain in the volatile mem ory until the end of the run Note It is not possible to modify the integral value during a run KR4i 5 11 Chapter 5 Using the Product 5 12 Configuration CQCS Option KR4i The Jouan KR4i can be configured to operate in set ways To set up the instrument s
64. ion of the supports liners during their insertion into the centrifuge using a bar code reader con nected to the centrifuge This stage is carried out after filling and balancing the supports ready to load them Thermo Fisher Scientific Chapter 5 Using the Product Thermo Fisher Scientific To activate this option select CONFIG from the stand by screen Select CQCS using V to display it in inverse video and press ENTER Enter the centrifuge serial number in the Jouan KR4i To do so select the CQCS option in the CONFIG menu then press START On the operator code display press the top right hand side key to configure the serial number USE KEYPAD TO SET USER IDENTIFICATION EHTER TO VALIDATE ESC TO ABANDON CENTRIFUGE ID ESC ENTER A A O E Enter the first 4 numbers of the serial number on the digital key board and validate with ENTER These first 4 numbers must stand between 1000 and 9000 non inclusive for example 3990 Then enter the last 4 numbers of the serial number on the digital keyboard and validate with ENTER These last 4 numbers must stand between 0 and 10000 non inclusive for example 2103 Press ENTER to validate the complete serial number The serial number registered in this example is 39902103 USE amp AND TO SELECT THE DESIRED TOPIC ENTERTO VALIDATE ESC TO ABANDON Esc CONFIGURATION LANGUAGE SETTING KR4i 5 13 Chapter 5 Using the Product 5 14 A
65. ity shall be detectable by survey equipment 2 A Decontamination Information Certificate shall be com pleted and attached to the instrument or part If an instrument or part to be serviced does not have a Decontam ination Information Certificate attached to it and in our opinion presents a potential radioactive or biological hazard our represen tative will not service the equipment until proper decontamination and certification has been completed If an instrument is received at our Service facilities and in our opin ion poses a radioactive or biological hazard the sender will be contacted for instructions as to disposition of the equipment Dis position costs will be borne by the sender Decontamination Information Certificates are included with these Operation Instructions Additional certificates are available from your local technical or customer service representative In the event these certificates are not available a written statement cer tifying that the instrument or part has been properly decontaminat ed and outlining the procedures used will be acceptable NOTE Thermo Fisher Scientific Service representatives will in dicate on a Customer Service Repair Report if decontamination was required and if so what the contaminate was and what procedure was used If no decontamination was required it should be so stated CHINA Tel 48621 68 65 45 88 Tel 86 10 58 50 35 88 AUSTRIA Tel 443 1 80 14 00 FRANCE
66. lates to the phenomenon of accelerated corrosion due to the effect of centrifugation when a corrosive chemical is in con tact with the alloy From the time when the aluminium alloy has been attacked by chemicals stress corrosion begins to appear As itis on a microscopic scale it is even more dangerous than a mac roscopic scale since it is invisible During centrifugation chemicals responsible for corrosion are also submitted to the tremendous g force which pushes them against the alloy This close contact facilitates the chemical reaction which occurs much faster than in a static situation Moreover centrifugal KR4i 5 21 Chapter 5 Using the Product 5 22 Cleaning KR4i force is very directional thus corrosion under stress creates with a very small amount of corrosive product straight microscopic fis sures Each centrifugation run makes the chemical migrate further and further A fissure although it is microscopic is a cut in the metal breaking the cohesion of the material As one weak link in a chain allows the chain to break so the microfissures break the chain of resistance of the accessory to centrifugal force As accessories are designed with high levels of safety rupture does not occur as soon as the first microfissures are produced Depending upon the location of the fissure disruption may occur before it reaches the external surface of the accessory The fissure creates a weakness which makes the accessory
67. le Is the centrifuge sitting firmly on the floor Is the rotor firmly attached to the shaft Is the seal between the bowl and motor attached Protective Elements Is the tachometer port plug in place Are the lid lock latches in place Are the lid lock eyes in place Is the imbalance detector in place Is the motor fixed firmly in place Are the lid springs and hinges in place Is the lid lock electrical system in place When the lid is locked is the bowl sealed Are the external panels firmly attached Thermo Fisher Scientific Chapter 6 Maintenance Functional verification of Thermo Fisher Scientific equipment Condition of materials Rotor Buckets Lid bowl seal Bowl motor seal Motor struts Lid latches Drive shaft Plastic bucket adapters Feet Dust on the condenser filter Foreign objects in the bowl Foreign objects in the winshield e Water in the bowl drain Condition of electrical connections Control panel attachments Attachments to power source condensor locks switches etc e Grounding and power cord Presence and function of safety systems Lid lock switch Lid must not open while rotor is moving Machine must not start while lid is open Lid open detection system Abnormalities during use e Noises Vibrations Temperature effects e Abrupt impacts Function of automatic manual shutdown systems Imbalance shutdown Lid open shutdown
68. led inside plastic bags from which the air has been evacuated KR4i 5 25 Chapter 5 Using the Product Chemical Compatibility Table for Rotors S Satisfactory D Discoloration but OK Tubes and P Pure chemical OK Accessories M Moderate resistance U Unsatisfactory X Explosion risk O No information d Less resistance if T gt 50 C t Unsatisfactory if T gt 50 C 2 S O 5 O a a o 2 A 5g s 9 o 8 E RICE E q E me 9 0 9 t 0 5 o 9 E a gt 28 9 q da o 9 ot go E w E DEP oO e E O A a 5 B2 2t 2 P8 gt gt gt gt gt S8 5856 3 5 v o0 0 gt gt O gt 6 6 o 6 6 S o Ex 60 OO OY 22 a aa a a 0 E P E gt Chemical Acetaldehyde S U U U O O O O M U M M O S S M S U O Acetamide O O O O O O O O S U S S S O O O O O O Acetic Acid 5 S M S S M S S S S S S D S S S S S M S Acetic Acid 20 96 S O O O O O O O S M S S S O O O O O O Acetic Acid 60 S U U U U S S M S U M D S S S S S U S Acetic Acid 80 96 S O O O O O O O S U St S M O O O O O O Acetic Acid Glacial S U U U U S O O S U M D M S S S S U S Acetic Anhydride S O O O O O O O Sd U U Sd U O O O O O O Acetone S U U U M M O U S U S M U S S M S U M Acetonitrile O O O O O O O O Mt U S Mt U O O O O O O Acetylene S O O O O O O O S O S S U O O O O O O Adipic Acid O O O O O O O O S S St S S O O O O O O Alanine S O O O O O O O S S U S U O O O O O O Allyl Alcohol O O U O S O O
69. less and less resistant to mechanical fatigue The corrosion by a small amount of corrosive product does not dis rupt the accessory but makes it mechanically weaker and weaker until it disrupts due to both centrifugal effort and number of cycles Because stress corrosion is largely invisible it is essential that rotors are scrutinised regularly paying particular attention to sus ceptible parts such as the base of the pockets the outer edges and the base of the rotor If fissures are suspected it is forbidden to use the rotor until it has been examined by a specialist Users must not use cleaning or decontamination methods different from those recommended without first having verified that these methods do not risk damaging the instrument or its accessories and obtained prior authorization from the manufacturer Ideally rotors should be washed after every use but at least weekly in warm water containing a few drops of mild non alkaline detergent domestic washing up liquid is ideal and EVERY TIME AFTER A SPILLAGE HAS OCCURRED Do not forget to wash the core of the rotor which comes into con tact with the drive spindle Each pocket of the rotor must be washed thoroughly using a small nylon brush DO NOT USE METAL WIRE BRUSHES Once the rotor is clean rinse it in running water preferably distilled Dry the rotor with a soft absorbant non woven cloth or tissue Dry ing may be finished off with warm air jet e g a hair dryer
70. mitted independently of the fatigue phenomenon rpm g 4 600 6 650 4 300 5800 3 900 5000 SAFETY ZONE 0 5000 10 000 15 000 20 000 25 000 Runs mel E GA J 2100 runs year 7 runs day 300 days year 1800 runs year 6 runs day 300 days year o a o 15 16 7 1500 runs year 5 runs day 300 days year Figure 5 5 P6 Liners 11178112 In KR4 22 rpm g 4 600 6650 4 300 3 900 5000 SAFETY ZONE 0 5000 10000 15000 20000 25000 Runs Years 2100 runs year 7 runs day 300 days year 1800 runs year 6 runs day 300 days year 15 16 7 1500 runs year 5 runs day 300 days year 5 10 0 Figure 5 6 P4 LINERS 11178074 IN KR4 22 High voltage is present behind the panels of the Jouan KR4i It is not necessary for the user to intervene inside the machine Only qualified technicians are authorized to do this Do not remove these panels unless the centrifuge has been first disconnected from the power outlet Never spin a rotor or its accessories at more than the maximum speed indicated in chapter 3 Do not attempt to override the lid interlock assembly Use only accessories designed for use in the Jouan KR4i Thermo Fisher Scientific Chapter 5 Using the Product Biological risks Thermo Fisher Scientific Load the rotor only in the recommended way and do not spin higher than the speed indicated on the rotor and accessories As the centrifuge starts to spin an improperly loaded roto
71. mple Under the previous conditions calculation of this integral is very simple In reality the centrifugation curve appears as follows Centrifugal acceleration g Aj 4 fl fl H 7 min Duration ru lt a pat Acceleration Constant speed Braking Figure 5 4 Actual centrifugation integral curve The integral of this curve is calculated by adding the elementary areas obtained by calculating the product elementary time interval x acceleration applied during this time interval The curve of two successive centrifugations for which the same speed and duration are applied are not identical Indeed according to sample weight temperature atmospheric moisture in the cham ber mains voltage fluctuations and motor wear the acceleration rate as well as constant speed phase may vary When constant speed durations are very limited or very long the previously described fluctuations may give rise to 5 to 10 varia tions in value of the integral The quality of the resultant separa tions is sometimes considerably modified as for example when preparing sub cellular fractions Integration control allows suppression of the effect of the various potential fluctuations Indeed rather than programming a given duration of centrifugation it is possible to set the desired value of the integral for a given speed or centrifugal accel
72. n and certification has been completed If an instrument is received at our Service facilities and in our opin ion poses a radioactive or biological hazard the sender will be contacted for instructions as to disposition of the equipment Dis position costs will be borne by the sender Decontamination Information Certificates are included with these Operation Instructions Additional certificates are available from your local technical or customer service representative In the event these certificates are not available a written statement cer tifying that the instrument or part has been properly decontaminat ed and outlining the procedures used will be acceptable NOTE Thermo Fisher Scientific Service representatives will in dicate on a Customer Service Repair Report if decontamination was required and if so what the contaminate was and what procedure was used If no decontamination was required it should be so stated INSTRUCTIONS When an instrument that has been used with radioactive patho genic or otherwise hazardous materials requires servicing by Thermo Fisher Scientific personnel either at the customer s labo ratory or at Thermo Fisher Scientific facilities the following proce dure must be complied with to insure safety of our personnel 1 The instrument or part to be serviced shall be cleaned of all blood and other encrusted material and decontamina ted prior to servicing by our representative No radioacti v
73. no site www thermo com WEEERoHS KR4i B 3 Declaration of conformity B 4 KR4i Thermo Fisher Scientific DECONTAMINATION INFORMATION CERTIFICATE Complete and attach to equipment BEFORE servicing DECONTAMINATION instructions on reverse PLEASE PRINT CERTIFIED BY TITLE POSITION PHONE FAX DEPARTMENT INSTITUTION ADDRESS CITY STATE ZIP INSTRUMENT SERIAL NUMBER ROTOR SERIAL NUMBER PART PART NUMBER HAZARDOUS CONTAMINANTS S DECONTAMINATION METHOD S DECONTAMINATION DATE DECONTAMINATION CERTIFIER S SIGNATURE DATE DECONTAMINATION INFORMATION CERTIFICATE Complete and attach to equipment BEFORE servicing DECONTAMINATION instructions on reverse PLEASE PRINT CERTIFIED BY TITLE POSITION PHONE FAX DEPARTMENT INSTITUTION ADDRESS CITY STATE ZIP INSTRUMENT SERIAL NUMBER ROTOR SERIAL NUMBER PART PART NUMBER HAZARDOUS CONTAMINANTS S DECONTAMINATION METHOD S DECONTAMINATION DATE DECONTAMINATION CERTIFIER S SIGNATURE DATE DECONTAMINATION INFORMATION CERTIFICATE Complete and attach to equipment BEFORE servicing DECONTAMINATION instructions on reverse PLEASE PRINT CERTIFIED BY TITLE POSITION PHONE FAX DEPARTMENT INSTITUTION ADDRESS CITY STATE ZIP INSTRUMENT SERIAL NUMBER ROTOR SERIAL NUMBER PART PART NUMBER HAZARDOUS CONTAMINANTS S DEC
74. ns of corrosion and or cracks Do not touch the electronic components of the rotor and do not make any changes to the electronic or mechanical com ponents Use only with rotors which have been expertly installed Use only with rotors which have been loaded properly Never overload the rotor Do not touch the electronic components of the centrifuge or alter any electronic or mechanical components Never start the centrifuge when the lid is open Never open the lid until the rotor has come to a complete stop and this has been confirmed in the display The lid emergency release may be used in emergencies only e g during a power failure Never use the centrifuge if parts of its cover panels are dam aged or missing Plug the centrifuge only into sockets which have been properly grounded Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific Exceptions to this rule are commercially available glass or plastic centrifuge tubes provided they have been approved for the speed or the RCF value of the rotor Please observe the safety instructions Please pay particular attention to the following aspects Location Place the centrifuge in a well ventilated environment on a solid level surface with adequate load bearing capacity KR4i V Foreword vi KR4i e Rotor installation make sure the rotor is locked properly into place before operating the centrifuge Especially
75. ojejooe eso nja2 N eung winiuiwiniy Jenae 5 29 O O O KR4i U U Sd S Mt Mt St Mt St St St Sd Mt Mt St St St St Sd Sd Mt Mt Mt St St St M S O O S O O S S O S Dextran Sulphate Diacetone Diacetone Alcohol o Dichlorobenzene p Dichlorobenzene Dichloroethane Dichlorophenol Diethylamine Diethyl Benzene Diethylene Glycol Diethylene Glycol Ethyl Ether Diethyl Ether Diethyl Ketone Dimethylacetamide Dimethylformamide Dimethylsulphoxide Dioxane Diphenyloxide Dipropylene Glycol Distilled Water Ethanol 50 Ethanol 95 Ethyl Acetate Ethyl Benzene Ethyl Benzoate Ethyl Butyrate Ethyl Chloride Ethylene Chloride Ethylene Glycol Ethylene Oxide Ethyl Ether Ethyl Lactate Ethyl Malonate Fatty Acids Thermo Fisher Scientific Using the Product Chapter 5 XOJ9A V UOMHA uiniuelr IezjeL uoper 99 S ssojureis euojins AJod euejAdoud AJod euoej uieAJod ayeuoqiedAjod 09dd 1euiojeAjoq uoJ N J JON Jeu M uujeg 9 J2MN 9so nijo2 9 eJ1 mq ojejooe eso nja2 N eung uniuiun v Jenae Thermo Fisher Scientific O O O O O O O O O U Sd Sd Sd U St Mt Sd Sd Sd Mt Sd St Sd Mt S Mt Mt Sd Sd Sd Sd Mt Sd Sd Sd Mt Sd U U O O U U U U M U KR4i U U S M S S U U U U U U S S O O O O Ferric Chloride Ferric
76. olour CAPACITY 3 sets of 4 inserts Q x HEIGHT MAX SET OF 4 min max RADIUS INSERTS mm mm N Tubes Volume Total inserts per ml tubes insert Set of 6 11175341 Salmon 12 20 1 5 240 11 35 257 buckets microtube Aen 11175343 Beige 12 26 5 312 12 7 67 107 257 for 2 inserts 1 sealing 11175344 Light 12 20 6 240 13 5 65 107 257 cap blue MUS 11175347 Cream 12 12 5 10 flat 144 17 5 65 112 257 bottom 11175358 White 12 7 12 flat 84 18 65 122 257 bottom 11175348 White 12 7 15 conical 84 18 90 122 259 Set of 6 11175349 Orange 12 7 15 vac 84 16 5 90 127 259 Seder 11175351 Coral 12 8 20 96 22 2 90 116 259 buckets 11178140 11175353 Darkred 12 4 50 48 30 5 90 122 259 bee a 11175354 Light 12 3 50 conical 36 30 90 117 259 ES blue microtitre plates 11175355 Green 12 1 100 12 45 90 132 255 11175356 Turquoise 12 1 250 flat 12 63 65 125 264 bottom 11175860 4 sets of 5 decanting rubber pads 10 5 12 1 mm for 11175343 inserts 1 8 KR4i Thermo Fisher Scientific Chapter 1 Presentation of product Table 1 6 RP6 AND RC6 COMMON ACCESSORIES IN 1178135 AND 11178136 BUCKETS Set of 6 standard buckets 11178136 Set of 6 sealed buckets 11178135 with cap CAT N Colour CAPACITY 3 sets of 4 inserts Q x HEIGHT MAX SET OF 6 min max RADIUS INSERTS mm mm N Tubes Volume Total inseris per ml tubes insert 11178208 Grey 6 37 3 5 222 12 7 50 107 266 254 11178210 Light 6 30 6
77. om your local technical or customer service representative In the event these certificates are not available a written statement cer tifying that the instrument or part has been properly decontaminat ed and outlining the procedures used will be acceptable NOTE Thermo Fisher Scientific Service representatives will in dicate on a Customer Service Repair Report if decontamination was required and if so what the contaminate was and what procedure was used If no decontamination was required it should be so stated INSTRUCTIONS When an instrument that has been used with radioactive patho genic or otherwise hazardous materials requires servicing by Thermo Fisher Scientific personnel either at the customer s labo ratory or at Thermo Fisher Scientific facilities the following proce dure must be complied with to insure safety of our personnel 1 The instrument or part to be serviced shall be cleaned of all blood and other encrusted material and decontamina ted prior to servicing by our representative No radioacti vity shall be detectable by survey equipment 2 A Decontamination Information Certificate shall be com pleted and attached to the instrument or part If an instrument or part to be serviced does not have a Decontam ination Information Certificate attached to it and in our opinion presents a potential radioactive or biological hazard our represen tative will not service the equipment until proper decontaminatio
78. ossible to access all pro gramming facilities The precool run can be halted at any time using the STOP keys If the program which is currently displayed is the one desired press a START key to run the program START O ONLO Otherwise another program can be recalled either by selecting the program number directly on the keyboard or by pressing Detail Appli which first shows all details of the current program then Scroll Appli same key to scroll through the memorized applica tion programs whose parameters are thus indicated on the screen Sustained pressure on the key causes rapid scrolling Thermo Fisher Scientific Chapter 5 Using the Product DETAIL 3800 om APPLI rs ease 00 15 emu mr COOL MODIFI NEW SAVE NeW START STANDBY SCREEN Once the required program is displayed simply press a START key to run the program START O O During a run the basic screen displays the 3 basic parameters with both set and live values A keypress on Expand Display presents a detailed screen showing all possible run parameters and the values of those selected both set and live A keypress on Basic Display returns the basic screen Note If the radius has not been defined by selecting a rotor and possibly modifying its radius to allow for the use of an adaptor the screen will not display the RCF It is impossible to call up a program or parameter from the key board 3800 3800 00 15 00
79. out lid N6 11178136 4900 rpm 4300 rpm 1 375 1 2 1 5 Cylindrical 7301 xg 5623 x Y N6CE Cylindrical 11178135 4900 rpm 4300 rom 1 375 1 2 1 5 Sealed 7301 xg 5623 x Y Rotor RHK4B Cat N 11178255 Bucket Cat N Max Max Bucket Load Load speed RCF speed RC weight kg density weight with lid F without kg bucket lid NHK4B 11174023 3300 rom 3 6 1 2 3 7 RIA 2301 xg Accessories Accessories have been marked with a maximum usable lifetime Thermo Fisher Scientific that must be respected For commentary concerning expected life time of accessories please refer to section 5 13 Only accessories specifically designed for use with the Jouan KR4i are authorized for use with this centrifuge The accessories used with the Jouan KR4i are RP4 windshielded 4 place swing out rotor without lid Designed for blood banking 8 quin or quad bags with or without filter or large volume processing 4 x 1600 ml It can be equipped with a lid The performance is 4250 rom 5513 x g with lid and 4000 rpm 4883 x 9 without lid RC6 windshielded 6 place swing out rotor without lid KR4i 1 5 Chapter 1 Presentation of product Table 1 1 Rotor RP4 Designed for multi purpose uses blood banking 6 quad bags large volume processing 6 x 1000 ml biochemistry cylindrical plastic inserts It can be equipped with a lid the performance then rises from 4300 rpm to 4900 rpm 5623 x g to 7301 x g RP6 windshielded 6 place swing out rotor
80. productos de Thermo Fisher Scientific afectados por la Directiva RoHS del ingl s Restriction of the use of certain hazardous substances in electrical and electronic equipment Restricci n de ciertas sustancias peligrosas en aparatos el ctri COS y electr nicos visite la p gina Web www thermo com WEEE RoHS Conformit RAEE Questo prodotto soggetto alle disposizioni della direttiva CE sui rifiuti di apparecchiature elettriche ed elettro niche Direttiva RAEE 2002 96 E contrassegnato tramite il sim bolo a lato Thermo Fisher Scientific ha preso accordi con aziende per la valorizzazione smaltimento e trattamento in tutti gli stati membri della UE per il riutilizzo o smaltimento e trattamento di que sti dispositivi Indicazioni su aziende per la valorizzazione smalti mento e trattamento in Germania cosi come informazioni su prodotti di Thermo Fisher Scientific che rientrano sotto la direttiva RoHS in ingl Restriction of the use of certain hazardous substan ces in electrical and electronic equipment Restrizione dell uso di determinate sostanze pericolose negli apparecchi elettrici ed elet tronici sono disponibili su Internet sotto www thermo com WEEE RoHS AEEA conformiteit Dit product is voorwerp van de bepalingen van de EU richtlijn betreffende afgedankte elektrische en elektroni sche apparatuur AEEA richtlijn 2002 96 Dit wordt aangeduid door het symbool hiernaast Thermo Fisher Scientific is met afvalverwer k
81. r could cause enough force to damage the drive shaft and the rotor chamber By the action of the turbulence created in the bowl a centrifuge is an aerosol generator In the case of products presenting a biologi cal risk you must take suitable precautions to prevent or reduce this risk and also train operators in the use of this instrument and its accessories in the context of their applications The following information should be given to all users including those persons using the equipment infrequently A ventilated centrifuge without built in refrigeration perma nently exhausts into the room air which has passed through its bowl while a refrigerated or thermostated centrifuge contains a relatively sealed bowl Thus the risks from the spread of aero sols are minimized in a refrigerated centrifuge and significant in a ventilated centrifuge Aerosols are produced naturally when using uncapped tubes or bottles They are also produced when a container deforms or breaks provoking a smaller or greater sample leak We draw your attention to the use of tubes used in angle rotors Even if the liquid level does not touch the cap when in a static state it could do so during centrifugation and leak You should there fore follow the instructions of the tube supplier concerning the fill level and sealing of the tube c Once a biological risk is known or suspected samples should be placed in a sealed container Should such containers no
82. responsible to respect the recommen dations made by the manufacturer with respect to installation use maintenance storage hygiene safety lifetime limits etc The manufacturer can be held responsible for the non conformity of the product For his part the user can bear a part of the respon sibility if he has not verified for himself that the product is in practi cal conformity even if it conforms to administrative guidelines The user is also held responsible if he does something himself to accentuate any possible problems with the product the responsi bility of the manufacturer can be reduced or eliminated taking into account all circumstances when damages are caused jointly by product problems and by the fault of the victim or by the fault of someone for whom the victim is responsible KR4i 1 11 Chapter 1 Presentation of product It is the responsibility of the user to train according to the instruc tions in this manual everyone who will use or service this equip ment It is also the responsibility of the user to verify the quality of each sample after centrifugation 1 12 KR4i Thermo Fisher Scientific Environmental Conditions for Transport Lifting and Transport Thermo Fisher Scientific Chapter 2 Shipment Transpor tation Transport conditions must be in accordance with the following rec ommendations if the machine is to function properly once installed and used under normal operating conditions Note I
83. rimary parameters Note The value introduced for the radius can be adjusted to allow for position within the tube such as at a boundary Maximum radii are quoted in the specifications tables chapter 1 9 R radius in millimetres N speed in r p m 1000 RCF gravitational acceleration g M add to memory MR memory recall KR4i 1 1 Chapter 1 Presentation of product 1 2 Drive System Refrigeration System KR4i Note To calculate actual results press the keys on a pocket calculator in the order shown Primary calculations Key sequence not valid for CASIO HP RCF xg 1 118RN2 Nx x1 118xR RCF Speed rpm 946 R RCF R x946 Radius mm RCE Nx x1 118 M RCF MR 1 118N Transformations Key sequence To determine actual g achieved at a different speed N22 ROF2 RCF1 55 Nx 2 x1 118 M RCF MR To determine actual speed required to achieve a different g at the same radius B RCF2 RCF2 RCF1 xN1 SOME RCF1 To determine actual speed required to achieve the same g ata different radius Ri R1 R2 xN1 N2 N1 gt R2 The rotor is driven by a three phase asynchronous motor The rotor is situated in the centre of an armour plated centrifugation bowl A control system ensures the correct drive Information about the refrigeration system Max pressure 26 bars Type of refrigerant R404A Total weight of refrigerant 1 5 kg The
84. rpm stainless steel 40 sec Osec 30sec 20sec 40sec 10sec 30sec Osec 30sec 10sec trays LKB trays 3 8 kg per liner The above values are given as a guide for the indicated load and nominal operating voltage 230 V These values can change if the voltage and or the load are different Braki NG The Jouan KRdi centrifuge allows a choice of 10 braking profiles which combine both rate and speed threshold Braking goes through two phases as follows Standard braking is applied from set point speed to the opera tor programmed speed threshold Programmed braking from speed threshold to O r p m Curve 0 1 2 3 4 5 6 7 8 9 Threshold 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 RP4 cylindrical buckets 18min 11min 7 min 5 min 5 min 3 min 2 min 2 min 2 min 2 min without lid N4C 1600 ml botte 20sec 50sec 50sec 40sec 50sec 20sec 40sec 20sec 20sec 0 sec 4000rpm d 1 0 Thermo Fisher Scientific KR4i 5 15 Chapter 5 Using the Product Curve 0 1 2 3 4 5 6 7 8 9 Threshold 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 RP4 cylindrical buckets 20 min 12min 8 min 6 min 4 min 3 min 3 min 2 min 2 min 2 min with lid N4C 1600 ml bottle 40sec 50sec 30sec 20sec 50sec 40sec Osec 40sec 40sec 20sec 4250rpm d 1 0 RP6 N6T buckets polypr 24min 16min 10 min 7 min 6 min 4 min 3 min 3 min 3 min 2 min with lid liner without partition 20 sec Osec 30sec 50sec 10sec 20sec 40sec 10sec Osec 30sec 3800 rpm triple bags d 1 0 RP6 N6T bu
85. s feet see previous instructions Connect the water pipes inside diameter 12 mm See Figure 4 2 e Tighten the connection on with strap to ensure water proofness Thermo Fisher Scientific Chapter 4 Installation Lid Opening Manual Lid Unlocking Manual lid opening procedure Thermo Fisher Scientific CD E ES to Strap Flexible pipe Water suction a Water discharge ac Figure 4 2 Water Cooled Connection The Jouan KRdi is equipped with a double locking safety device which stops the machine starting if the lid is not completely closed and prevents lid opening before the end of rotation When the centrifuge is switched on wait until display returns to the stand by mode then pull forwards the latch lever located on the right hand side of the unit the lid is automatically unlocked and opens In the case of a mains power cut opening of the lid is prevented by the lid locking safety device It is recommended to wait for the mains to be Switched back on so that this safety device enables the lid to be unlocked During rotation the slightest leak from a sample is enough to cre ate aerosols Rotation of the rotor can only be detected when the machine is switched on Opening the lid manually if the rotor is still rotating would disperse the aerosols in the environment Rotating parts are also a risk as these could come in contact with the user or be ejected There is particularly high risk of injury if T
86. stream of liquid shooting out of refrigeration system The refrigeration system is enclosed by the external panels of the centrifuge There is no risk of explosion in this case Thermo Fisher Scientific Chapter 1 Presentation of product Internal and External Markings of Centrifuge Responsibility Thermo Fisher Scientific Note The following table lists and describes the notices and markings found on the interior or exterior of the machine Type of notice Interior Specific Loca Description or marking exterior tion Product Exterior Rear panel Name of manu information label facturer Danger symbol Exterior Rear panel Reference and serial numbers Identification Exterior Working surface Main character Sticker next to bowl istics of visible when lid machine is open Sticker ISO 9001 Exterior Front panel Beware of certification electric shock precaution unplug the machine prior to performing any service Environmental Exterior Front panel Name of protection machine Sticker Danger symbol Exterior Right panel next Reference and to opening used serial numbers for manual lid unlock procedure Other stickers Interior On certain Information for the purpose component parts concerning of information or not made by Thermo Fisher warning Thermo Fisher Scientific s ISO Scientific certification Interior Compressor Guaranteed CFC free helps to The user of this centrifuge is
87. sword for this application accepts 0 to 9999 inclusive via the keypad then select ENTER or to select NONE for no password protection to save the application If the user chooses to store his application in a used but unpro tected memory the old values will be replaced by the new values If he chooses to store his application in a used but protected memory he must first enter the password to gain access to the memory In both cases he can password protect the new application Note The Save Appli function will only operate if one or more parameter values have been modified The unit is delivered without any passwords installed The use of a password prevents easy overwriting of a protected application pro gram Thermo Fisher Scientific Chapter 5 Using the Product To Modify A Program Thermo Fisher Scientific We suggest that you write down the password in case you forget it In the case of problems there is a special procedure to enable the protection to be overridden This same procedure is used to clear one or more memories to eliminate unwanted applications After Save Appli has been selected the file screen is displayed To eliminate the password protection and erase a program use the lt and keys to move to the required memory closed file icon and select CE Use lt and CE to clear any other memories Moving the cursor back over selected memories will then show them to be EMPTY as indicated in the right hand s
88. system is CFC free and is without inflammable fluid Thermo Fisher Scientific Chapter 1 Presentation of product Dimensions and Weight Height lid open 650 1670 mm Height lid closed 915 935 mm Width 798 mm Depth 905 mm Weight uncrated 346 kg Weight crated 380 kg Rquired surface area 1 2 m safety zone Electrical Specifications au Mains supply 230 V 10 50 Hz or 208 230 V 60 Hz Fuses Main pcb T 5 x 20 160 mA 26387014 Supply pcb F1 T 5x20 63 mA 86001807 Supply pcb F2 T 5 x 20 250 mA 34000207 Motor 2000 W at 4400 rpm Refrigeration 1 5 hp 1100 W Average power 3500 VA Maximum power 4500 VA Performance KRdi Max speed 4900 rpm Max RCF 7300 x g Max capacity 4x 1600 ml 6 x 1000 ml 8 or 12 quin or quad bags with or without filter 4 x 2160 ml 6 x 2000 ml buckets Speed Range 500 to 4900 rpm Step 10 rpm Accuracy 10 rpm RCF xg Range 80 to 7300 x y Step 1xg Pr cision lt 0 5 Thermo Fisher Scientific KR4i 1 3 Chapter 1 Presentation of product KRdi Timer Range 0 to 99 h 59 min 59 sec Step hold position 1 min 1 sec Energy Max density 1 2 g ml Max load on drive shaft 37 kg Max energy 129050 J Note The maximum rotational energy is not systematically that obtained with the maximum permissible load on the drive shaft KR4i Integrator Range 0 to 999 x 10 m rd sec Step 1 m rd sec Acceleration rates 10 Braking rates
89. t be avail able sealed accessories should be used such as buckets or angle rotors with sealed lids Sealed rectangular buckets 11178140 are certified by CAMR Porton Down for their containment efficiency Unsealed buckets 11178138 are sealed if fitted with lid 111782196 Duration of aerosol presence when aerosols are created in a centrifuge bowl they persist in the air for a period of 3 5 minutes after the rotor stops moving Indeed the action of opening the lid itself provokes the dispersion of aerosols in the environment KR4i 5 39 Chapter 5 Using the Product 5 40 KR4i When an elevated risk of aerosols or of breakage is perceived the accessories should be handled using gloves and opened in an environment ensuring the protection of the operator safety cabinet glove chamber wearing a mask even if they are apparently sealed Those who use or service this equipment must wear appropri ate personal protective gear given the type of solutions being centrifuged Dangerous substances It is recommended tat the user takes all the necessary precautions when centrifuging substances known for their toxicity or radioactiv ity or when they are contaminated by microbiological pathogens notably material of Risk Group Il as identified in the Biological Safety Manual for Laboratories edited by the World Health Organ isation See also chapter contamination hazard Thermo Fisher Scientific Daily Cleaning W
90. ted work periods Machine Alarms N Message 1 TACHOMETER FAILURE error code 0 Description Speed measured at value other than zero when the centrifuge is powered up Action Wait for the motor to stop and the lid to unlock before trying to restart the machine 2 TACHOMETER FAILURE error code 1 No signal from the tachometer Contact Thermo Fisher Scientific technical service 3 TACHOMETER FAILURE error code 2 4 TACHOMETER FAILURE error code 3 5 34 KR4i Variation of tachometer signal is too sharp Inconsistent tachometer signal Contact Thermo Fisher Scientific technical service Contact Thermo Fisher Scientific technical service Thermo Fisher Scientific Chapter 5 Using the Product N Message POWER FAILURE Description Motor slip abnomally high Action Contact Thermo Fisher Scientific technical service 6 LID UNLOCKED Attempt to start the centrifuge Close the lid completely and try again to start the while the lid is open centrifuge If the problem persists contact Thermo Fisher Scientific technical service 7 LID UNLOCKED Warning this is a major problem Wait for the rotor to come to a complete stop If The lid has been opened while problem persists contact Thermo Fisher Scientific the rotor is in motion technical service 8 IMBALANCE Buckets samples are not Wait until the rotor has stopped and the lid has been balanced unlocked Rebalance the samples then tr
91. the heat can damage the samples At 45 C the run is automatically stopped Other safety features are also included in the centrifuge There is no corresponding message but these features ensure safe use of your centrifuge overspeed safety Current limitation armour plating Caution The Jouan KR4i Centrifuge is not explosion proof Using this centrifuge with explosive samples is entirely at the user s own risk The use of inflammable or explosive materials or materi als which give a violent chemical reaction is strictly forbidden Centrifuge buckets are manufactured from aluminium alloys of high mechanical strength and elasticity During centrifugation their own mass and most importantly the load that they contain submit them to a stress load varying from some hundreds of kilograms to sev eral tonns This stress varies according to the total mass and increases considerably with speed it doubles with a speed increase of only 41 It is therefore essential to rigorously respect the limits of soeed and load indicated in the instructions and even on the accessories themselves Exceeding these limits causes weakening followed by a total rup ture of the metal Thermo Fisher Scientific Chapter 5 Using the Product Mechanical fatigue Corrosion Thermo Fisher Scientific The phenomenon This normal physical phenomenon appears when a mechanical force is applied and relaxed repeatedly The accumulation of alter nate
92. tor mounting screw Use the hub puller supplied place its screw above the top of the motor shaft Tighten the puller screw clockwise until the rotor is pulled out completely use wrench supplied if necessary To reinstall the rotor refer to section 4 7 This operation must only be done by trained and qualified staff The Jouan KR4i and buckets used with the Jouan KR4i are very heavy When handling these accessories take appropriate precau tions to prevent back or other skeleto muscular injury Changing of fuses must not be carried out by the operator but by a qualified engineer who will diagnose the fault before replacing the fuses Fuses Main pcb T 5x20 160 mA 26387014 Supply pcb F1T5x20 63mA 86001807 Supply pcb F2T5x20 250 mA 34000207 See chapter 4 KR4i 6 5 Chapter 6 Maintenance 6 6 KR4i Thermo Fisher Scientific Thermo Fisher Scientific Chapter 7 Disposal of Product The following disposal recommendations are valid for the machine or its accessories at the end of its useful life or the parts that must be disposed of because they are worn out or degraded during use Prior to disposal the machine and accessories must be decontam inated In this respect the user is required to submit a decontamination cer tificate completed and signed to the company or persons who are in charge of disposal The elements of the product that may become noxious during the life of the product are the following
93. wing out rotors should be loaded with a full compliment of buckets to maintain correct bal ance and safety for the rotor Use only centrifuge rated glass or plastic ware tubes vials or bottles Although the Jouan KR4i can compensate for an imbalance of more than 50 grams sample containers should be balanced to avoid any long term effects of vibration and assure good sample separation If you intend to use old accessories on a new centrifuge please contact your Thermo Fisher Scientific representative for advice Thermo Fisher Scientific Chapter 5 Using the Product Controls and Indicators Thermo Fisher Scientific A major imbalance can seriously damage the rotor and the centrifuge Never put liquid directly into the buckets Refer to the guide on the use of refrigerated centrifuges in blood transfusion The mains switch is located on the lower left side of the front panel The control system includes A dot matrix graphic LCD screen able to display numbers let ters and special characters in addition to animated graphics Eight keys surrounding 2 sides of the screen have their func tions determined according to the name displayed on the adja cent part of the screen Not all keys are necessarily operational at any one time and more than one key can operate the same function when the name of the function is displayed across a wide area of the screen next to these keys n addition a standard numeric keypad
94. y to start the run again If problem persists contact Thermo Fisher Scientific technical service 9 MOTOR Motor temperature is too high Contact Thermo Fisher Scientific technical service OVERTEMPERATURE 10 CHAMBER Bowl temperature is higher than May be because the ambient temperature around OVERTEMPERATURE 43 C the centrifuge is too high Verify that the centrifuge is installed far enough away from heat sources and that there is enough space around the machine to allow proper ventilation If problem persists contact Thermo Fisher Scientific technical service COMMUNICATION FAILURE Thermo Fisher Scientific only if the centrifuge is in CQCS mode The centrifuge has not Verify that the supervision application is active on the supervisor PC received a request from the supervisor PC for over 2 minutes Safety At any time when the instrument is able to detect and inform the operator of a fault condition the screen will display a special mes sage from among the following Imbalance Important vibrations which can damage the accesso ries the unit and the samples have been detected The cause is the bad balancing of the inserts As soon as the motor stops open the centrifuge and redistribute the samples to produce an equal weight on diametrically opposite sides of the rotor and for swing out rotors on each side of the trunnion for each bucket Using the grease supplied ensure that the trunnions are regularly lubricated

KR4i Large Capacity Refrigerated Centrifuge

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