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1200 Infinity Series Multisamplers

1. 6 Take the metering device Push against the rear side of the metering device and rotate a quarter left to release the bayonet lock 158 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Remove the Metering Seal 7 Now you can separate the analytical head and head body 8 Remove the piston out of the head body 1200 Infinity Series M ultisamplers User Manual 159 9 Maintenance Remove the Metering Seal 9 Inspect the piston for cleanliness and scratches 10 Take the analytical head and remove the three screws on the rear side which holds the support ring in place Check the support ring for any damages If dirty Clean the piston with an appropriate solvent If scratched Replace the piston by a new one 160 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Remove the Metering Seal 11 Carefully remove the metering seal using the steel side of the insert tool Clean the chamber with an appropriate solvent and ensure that all particulate matter is removed 1200 Infinity Series M ultisamplers User Manual 161 9 Maintenance Install the M etering Seal When After removing the metering seal Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2392 4mm Hex key 01018 23702 Insert tool Cleaning tissue and appropriate solvent like isopropanol or methanol Parts required p n Description 1 090
2. 1200 Infinity Series M ultisamplers User M anual 143 9 Maintenance Install the Needle Assembly When Tools required Parts required OR Preparations When the limit in the needle into seat counter in the EM F is exceeded or when needle shows indications of damage blockage or leaks p n Description 8710 0510 Wrench open 1 4 5 16 inch p n Description 1 64267 87201 Needle Assembly 1 G4267 87210 Needle Assembly slotted for high injection volumes In order to avoid leaks stop the pump running and remove the tubings from the solvent bottles If available close the shutoff valves Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Do not open the safety lock of the needle assembly gt Be careful working at the z robot gt Wear safety goggles when removing the needle assembly 144 It is recommended to always exchange the needle assembly and the needle seat at the same time to prevent premature leakage 1200 Infinity Series M ultisamplers User Manual Maintenance 9 1 Install the loop capillary on top of the needle cartridge CAUTION 1 and tighten the fitting hand tight 2 CAUTION Blockages gt Do not overtighten the fitting A quarter turn should be sufficient 2 Useal 4 inch wrench to tighten the fitting of the loop capillary 1200 Infinity Series M ultisamplers User Manual 145 9 Maintenance Install the Need
3. The Agilent Lab Advisor software is available in two versions Lab Advisor Basic Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Infinity II Series pump The Lab Advisor Advanced features can be unlocked by purchasing a license key and include real time monitoring of instrument actuals all various instrument signals and state machines In addition all diagnostic test results calibration results and acquired signal data can be uploaded to a shared network folder The Review Client included in Lab Advisor Advanced allows to load and examine the uploaded data no matter on which instrument it was generated This makes Data Sharing an ideal tool for internal support groups and users who want to track the instrument history of their analytical systems The optional Agilent Maintenance Wizard Add on provides an easy to use step by step multimedia guide for performing preventive maintenance on Agilent 1200 Infinity and Infinity II Series instruments The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files 1200 Infinity Series M ultisamplers User Manual 107 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 108 1200 Infinity Series M ultisamplers User Manual 1200 Infinity Series Multisamplers User Manual e e m a
4. 36 1200 Infinity Series M ultisamplers User Manual Table 2 Type Specification Site Requirements and Specifications 2 Agilent 1290 Infinity II M ultisampler G7167B Performance Specifications Comment GLP features Housing Metering device Early maintenance feedback EM F for continuous tracking of instrument usage with user settable limits and feedback messages Electronic records of maintenance and errors All materials recyclable Metering device in high pressure flow path 1 Performance Specifications G7167A only for the integration in an Infinity LC setup Table 3 Agilent 1260 Infinity M ultisampler G7167A Performance Specifications Type Specification Comment Injection range for 0 1 100 uL optional 20 uL 40 uLin 0 1 uL Up to 600 bar using 100 uL analytical head Single needle increments default instruments 0 1 500 uLor 900 uL in 0 1 uL increments Pressure range up to 400 bar due to 900 uL Injection range for Dual needle instruments Precision Pressure range Sample viscosity range default 0 1 120 uL in 0 1 uL increments with 1290 Infinity large volume injection kit hardware modification required G4216 68711 0 1 500 uL 1500 uL in 0 1 uL increments with 100 uL upgrade kit hardware modification required G7167 68711 0 20 uL optional 40 uL in 0 1 uL increments default 0 100 uL optional 500 uL in 0 1 uL increments default lt 0 15 RSD
5. Control and data evaluation Local Control Communications Safety and maintenance lt 0 003 30 ppm M ultisampler Standard Using the following conditions lt 0 0009 9 ppm M ultisampler M ultiwash Column Agilent ZORBAX SB C18 2 1x 50 mm1 8 mm 827700 902 Mobile Phase A 0 1 TFA in water B 0 1 TFA in Acetonitrile Isocratic B 35 Flow rate 0 5 mL min Temperature 25 C e Wavelength 257 nm Sample 1200 ng uL Chlorhexidine dissolved with mobile phase A 1 uL injected and measured on G4212A DAD Wash solution H20 with 0 1 TFA 5s Outer needle wash and seat backflush for Carryover reduction with up to 3 different solvents Agilent Open Lab CDS A 02 01 or above A 02 02 supports Sample Entry Ul M assHunter QQQ B 07 00 SP1 or above M assHunter QTOF B 05 01 SP3 or above Lab Advisor B 02 05 or above ICF for 3rd party SW control A 02 01 or above LC and CE Drivers A 02 10 or above Agilent Instant Pilot G4208A B 02 17 or above currently not supported official release 2015 Controller area network CAN Local Area Network LAN ERI ready start stop and shut down signals Extensive support for troubleshooting and maintenance is provided by the Instant Pilot Agilent Lab Advisor and the Chromatography Data System Safety related features are leak detection safe leak handling leak output signal for shutdown of pumping system and low voltages in major maintenance areas
6. USB A F USB Mini B M OTG Module to Flash Drive 1200 Infinity Series M ultisamplers User Manual 221 11 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent M odule to 35900 A D converters p n 35900 60750 35900 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog 222 1200 Infinity Series M ultisamplers User Manual Identifying Cables 11 Agilent M odule to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module o AN Shield Shield Analog _ lt Center Center Analog Agilent M odule to General Purpose p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog E 3 Red Analog ee 1200 Infinity Series M ultisamplers User Manual 223 11 Identifying Cables Remote Cables ERI Enhanced Remote Interface 5188 8029 ERI to general purpose p n 5188 8029 pin EnhancedRemote Classic Remote 101 START REQUEST D Sub female 15way Q user s view to connector 2 102 STOP sos 3eses8e8s 3 103 READY I 104 POWER ON oh A E 5 105 NOT USED 73D 0 ypyeoug s 6 106 SHUT DOWN lt lt 00 ou S 7 107 START 8 108 PREPARE 9 lwire DATA 10 DGND 11 5V ERI out 12 PGND 13 PGND 14 24V ERI out 15 24V ERI out 5188 8044 ERI to ERI Connector D_Submi
7. te Agilent Technologies Notices Agilent Technologies Inc 2014 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number G7167 90001 Rev B Edition 12 2014 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this do
8. 1200 Infinity Series Multisamplers User M anual J e e c 0 ee 13 one LAN Configuration Setting up the module in a LAN environment 252 Connecting the module viaLAN 253 This chapter provides information on connecting the detector to the Agilent ChemStation PC a Agilent Technologies 251 13 LAN Configuration Setting up the module in a LAN environment It is not recommended to connect a system via an autosampler The detector is producing the most data in the stack followed by the pump and it is therefore highly recommended to use either of these modules for the LAN connection 252 1200 Infinity Series M ultisamplers User Manual LAN Configuration 13 Connecting the module via LAN If the module is being operated as a standalone module or if a connection via LAN is required regardless of above mentioned recommendation a G1369B C LAN card has to be used For installation and configuration see the G1369B C documentation 1200 Infinity Series M ultisamplers User Manual 253 13 LAN Configuration Connecting the module via LAN 254 1200 Infinity Series M ultisamplers User Manual 1200 Infinity Series Multisamplers User Manual e 14 009 Appendix General Safety Information 256 General Safety Information 256 Safety Standards 256 General 256 Before Applying Power 257 Ground the Instrument 257 Do Not Operate in an Explosive Atmosphere 258 Do Not Remove the Instrument Cover 258 Do Not M odify the
9. Replace the Rotor Seal 9 Reconnect all capillaries to the proper injection valve ports with a 1 4 inch wrench 10 Close the front door L 11 Perform a pressure test 156 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Remove the M etering Seal When When poor injection volume reproducibility or when metering device analytical head is leaking Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2392 4mm Hex key 01018 23702 Insert tool Parts required p n Description 1 0905 1717 Metering seal 40 uL for 40 uL analytical head 1 0905 1719 Metering seal 100 uL for 100 uL analytical head 1 5067 5920 Piston ceramic If previous piston is scratched 1 Inthe Instant Pilot start the maintenance mode and 2 Open the front door select Change metering device function OR In the Agilent Lab Advisor software select Service amp Diagnostics in the system screen Tools gt Maintenance Positions gt Change Metering Device click start and wait until the metering device is in maintenance position 1200 Infinity Series M ultisamplers User Manual 157 9 Maintenance Remove the Metering Seal 3 Disconnect all capillaries from the metering device 4 To release the bayonet lock push 1 and rotate 2 the analytical head a quarter left Then you can pull and detach the analytical head assembly from the actuator
10. lower or both semi circle status indicators are ON indicates the rear or front position of the drawer or both positions are loaded with a sample containers When semi circle indicators are blinking the robot interacts with a drawer NOTE During blinking of the drawer status indicator Do not try to open the drawer at this point Drawer status indicator 1200 Infinity Series Multisamplers User M anual 47 3 Using the Module Insert vial trays wellplates Insert vial trays wellplates 3 4 Ensure correct seat and orientation of vial trays wellplates 48 1200 Infinity Series M ultisamplers User Manual UsingtheModule 3 Remove vial trays wellplates Remove vial trays wellplates 1200 Infinity Series Multisamplers User M anual 49 3 Using the Module Installing the Sample Cooler Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at
11. port seat 4 y Z Metering device Multisampler Column Figure 18 Valve in bypass washing needle 1200 Infinity Series M ultisamplers User Manual 91 5 Optimizing Performance How to Configure the Optimum Delay Volume Table 10 Schematic of injection steps in 1290 Infinity II M ultisampler db Solvent Pump Wash 4 q Z Metering PE device Column Sampling loop Multisampler Figure 19 Valve in mainpass sample injected veel device Multisampler Column Figure 20 Valve in bypass needle backflush M ultiwash 92 1200 Infinity Series M ultisamplers User Manual Optimizing Performance 5 When using ADVR it should be noted that the gradient has already started at the pump at the instant of injection The question should be asked whether the gradient has already reached the autosampler in which case a small step in the gradient will result This happens when the delay volume is less than the flush out volume and is not necessarily a problem but may be a factor to be considered in a method transfer With a flush out factor of 5 and an injection volume of 10 ul the autosampler will allow 50 ul to pass through before switching to bypass which with a delay volume of 50 ul means the gradient just reached the injection valve Smaller injection volumes will have no effect but for larger injection volumes this will introduce a small step in the gradient The flow rate in use will also have
12. 12 6 x 15 6 x 18 4 x depth inches Line voltage 100 240 V 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC EN CSA UL 180 VA 180 W 4 40 C 39 104 F 40 70 C 40 158 F lt 95 r h at 40 C 104 F Up to 3000 m 9842 ft Up to 4600 m 15092 ft Non condensing For storing the module Installation category Il Pollution degree 2 For indoor use only 34 1200 Infinity Series M ultisamplers User Manual Performance Specifications Site Requirements and Specifications 2 Table 2 Agilent 1290 Infinity II M ultisampler G7167B Performance Specifications Type Specification Comment Injection range for 0 1 100 uL optional 20 uL 40 uL in 0 1 uL Up to 1300 bar using 100 uL analytical head Single needle increments default instruments 0 1 500 uLor 900 uL in 0 1 uL increments Pressure range up to 400 bar due to 900 uL Injection range for Dual needle instruments Precision Pressure range Sample viscosity range Sample capacity Injection cycle time default 0 1 120 uL in 0 1 uL increments with 1290 Infinity large volume injection kit hardware modification required G4216 68711 0 1 500 uL 1500 uL in 0 1 uL increments with 100 uL upgrade kit hardware modification required G7167 687
13. 4 le q with Agilent Open Lab ChemS tation Table8 The Autosampler User Interface gt Multisampler om Module Status shows Run Ready Error state and Not Ready text or Error text eo idie Error Red Not ready yellow Ready green Pre run Post run purple Run blue Idle green Offline dark gray Standby light gray Injection Volume 0 00 pL Sample Location EMF Status shows Run Ready Error state and Not Ready text or Error text Offline gray Ok No Maintenance required green EMF warning Maintenance might be required yellow EM F warning Maintenance required red Injection Volume 0 00 pL Sample Location Control Settings These settings are available via right click on the Active Area of the ALS GUI 1200 Infinity Series M ultisamplers User Manual 81 4 Preparing the module Setting up the Autosampler with Agilent Open Lab ChemStation Table9 Control settings Missing Vessel The handling of missing vessels can be configured Illumination Switch on off the internal illumination Linked Pump To configure which pump delivers flow to the Autosampler Prime Flush Pump Priming the Needle wash flush pump Work space Clear the workspace gt Immediately At the End of the Analysis and Never M ethod Parameter Settings These settings are available via Menu gt Instrument gt Set up Instrument M ethod Mu
14. Check and Quang Modde rto G7IG7A__ 12 ena i r 4 bev ware Vechten Figure 24 Maintenance Positions Change Needle Assembly The Sample handler is positioning the needle assembly so that there is easy access for changing needle assembly or needle seat The position is far to the left of the needle parkstation and the current to the motors are off so that the Z drive of the robot can be moved while servicing the module 1200 Infinity Series M ultisamplers User Manual Test Functions and Calibration 8 Maintenance Positions Maintenance Positions Task Selection Change Rotor Seat No 2a Change Loop Ne Change Metering Device No J Press the Stat gt gt button in order to begin Procedure Change Saat Ves Change the coat Qhange the noede Change Neede Yes 2j Leave the service mode Pafom auto referencing Reset EMF Courters Figure 25 Change Needle Asssembly Change Sample Loop Capillary The Change Loop command positions the Z drive of the robotarm far to the left of the needle parkstation to enable easy exchange of the sample loop cartridge Task Selection range Rotor Seat No Set gt gt Qhange Loco Yes Change Metewng Device No SD Prove ine Sat gt gt buton in orderto begn Procedure Charge Seat No Change the loop Leave the sanoe mose Qharge Need Ne gt j Figure26 Change Sample Loop Capillary 1200 Infinity Series M ultisamplers User M anual 125 8 126 Test
15. Handling For correct installation of your system contact your Agilent service representative 1200 Infinity Series M ultisamplers User Manual 63 4 Preparing the module Preparing the M ultisampler 64 For best performance of the multisampler When using the multisampler in a system with a vacuum degassing unit shortly degas your samples before using them in the multisampler Filter samples before use in a 1200 Infinity Series system Use High pressure filter kit 5067 4638 for inline filtering When using buffer solutions flush the system with water before switching it off Check the multisampler plungers for scratches grooves and dents when changing the piston seal Damaged plungers cause micro leaks and will decrease the lifetime of the seal Solvent Information Observe recommendations on the use of solvents see Solvent Information on page 66 Priming and Purging the System When the solvents have been exchanged or the system has been turned off for a certain time for example overnight oxygen will re diffuse into the solvent channel Therefore priming and purging of the system is required before starting an application Table6 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of the system When switching between Isopropanol Best solvent to flush air out of reverse phase and normal the system phase b
16. Maintenance This chapter describes the maintenance of the Multisampler 10 Parts for Maintenance and Upgrade or Options This chapter provides information on parts material required for the module 11 Identifying Cables This chapter provides information on cables used with the modules 12 Hardware Information This chapter describes the module in more detail on hardware and electronics 13 LAN Configuration This chapter provides information on connecting the detector to the Agilent ChemStation PC 14 Appendix This chapter provides addition information on safety legal and web 1200 Infinity Series M ultisamplers User Manual Contents Contents 1 Introduction 9 Product Description G7167B 10 Product Description G7167A 11 Features G7167B 12 Features G7167A 13 Overview of theModule 14 Multisampler Principle 16 System Overview 22 Site Requirements and Specifications 29 Site Requirements 30 Physical Specifications 34 Performance Specifications 35 Physical Specifications of the Sample Cooler 40 Using the Module 43 Magnets 44 Turn on off 45 Status indicators 46 Drawer Status Indicator 47 Insert vial trays wellplates 48 Remove vial trays wellplates 49 Installing the Sample Cooler 50 Transporting the Multisampler with a Sample Cooler Installed 58 Preparing the module 61 Leak and Waste Handling 62 Preparing the Multisampler 64 Solvent Information 66 Recommended Mats and Vials 71 Capi
17. Run The final step is the inject and run step The six port valve is switched to the mainpass position and directs the flow back through the sample loop which now contains a certain amount of sample The solvent flow transports the sample onto the column and separation begins This is the beginning of a run within an analysis In this stage all major performance influencing hardware is flushed internally by the solvent flow For standard applications no additional flushing procedure is required 1200 Infinity Series M ultisamplers User M anual 19 1 20 Introduction dy Solvent Pump Sampling loop Wash pump Wash Needle Waste Esl port seat Metering device Column Multisampler Figure6 Valve in mainpass sample injected Needle seat back flush After the injection to reduce the carry over for very sensitive analysis the needle seat can be flushed by an integrated flush pump with up to 3 different solvents which may have different properties and solvent strengths As soon as injection valve is in bypass mode the flush pump delivers some solvent during a defined time to clean the needle seat The back flushing solvent will be guided into the waste line attached on the needle wash port At the end of this process the injection valve switches back into the mainpass position ready for the next injection The last rinsing step should always include the mobile phase as solvent to get the initial co
18. are monitored continuously by the Multisampler processor The processor defines specific time windows and mechanical ranges for each movement If a specific step of the sampling sequence is not completed successfully an error message is generated Solvent is bypassed from the Multisampler by the injection valve during the sampling sequence After the required sample container was automatically loaded from the sample hotel and placed on the central workspace The Needle assembly moves via robot to the desired sample position and is lowered into the sample liquid in the sample to allow the metering device to draw up the desired volume by moving its plunger back a certain distance The needle assembly is then raised again and moved to the needle park station onto the seat to close the sample loop Sample is applied to the column when the injection valve returns to the mainpass position at the end of the sampling sequence The standard sampling sequence occurs in the following order The robot loads the required sample container on the central workspace The injection valve switches to the bypass position The plunger of the metering device moves to the initialization position The robot couples into the needle assembly from the needle parkstation The robot unlocks the needle assembly and moves up a ur W N Pe The coupled needle assembly robot moves to the desired sample vial or well plate position on the central workstation 7 The needle low
19. concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 248 1200 Infinity Series M ultisamplers User Manual Hardware Information 12 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user sele
20. flows directly to the column through port 6 The standard injection starts with draw sample from vial wellplate from the central workstation In order to do this the needle assembly moves via robot to the desired sample position and is lowered into the sample liquid 1200 Infinity Series M ultisamplers User M anual 17 1 18 Introduction in the sample to allow the metering device to draw up the desired volume by moving its plunger back a certain distance The needle assembly is then raised again and moved to the needle park station onto the seat to close the sample loop In case of an injector program several steps are interspersed at this point dy Solvent Pump Sampling loop Wash Wash Needle Waste port seat 4 gt Pd Metering device Multisampler Column Figure4 Valve in bypass drawing sample Flush the Needle Before injection and to reduce the carry over for very sensitive analysis the outside of the needle can be washed in a flush port located behind the injector port As soon as the needle is on the flush port a wash pump delivers some solvent during a defined time to clean the outside of the needle At the end of this process the needle assembly returns to the needle port 1200 Infinity Series M ultisamplers User M anual Introduction 1 Solvent Pump Sampling loop Wash Needle Waste port seat y Metering device Multisampler Column Figure5 Valve in bypass washing needle Inject and
21. grounds greenhouse effect CAUTION General hazards and improper disposal Improper disposal of the media and components used pollutes the environment gt The breakdown of the sample cooler unit must be carried out by specialist refrigeration company gt All media must be disposed of in accordance with national and local regulations gt Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance 264 1200 Infinity Series M ultisamplers User M anual Appendix 14 Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 1200 Infinity Series Multisamplers User Manual 265 14 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A At Operator Position Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 266 1200 Infinity Series M
22. in turn With Agilent s dual needle design cycle time is about 5 seconds With the multi wash capability you can reduce carryover to less than 9 parts per million Status indicator Needle assy Hotel drawer Leak drain Power switch Figure2 Overview of the Multisampler 1200 Infinity Series Multisamplers User M anual 11 1 Introduction Features G7167B 12 Unmatched flexibility You choose how you want to introduce samples for injection whether you prefer vials microtiter plates or any combination of formats Sample drawers are available in three heights and you can mix shallow drawers with deeper ones to accommodate different sample sizes High capacity Using shallow well plate drawers the 1290 Infinity II Multisampler takes a maximum load of 16 microtiter plates and up to 6144 samples the most of any single system Seamless automation Internal robotics move microtiter plates and other sample containers from the sample hotel to the central workspace for sample processing steps and injections Dual needle injection By running samples alternately through one or the other injection path you can reduce cycle times to mere seconds virtually eliminating conventional wait times whether for large volume loadings or flushing procedures Scalable injection volumes The Agilent unique dual needle integ setup also enhances flexibility by providing two differently optimized injectors in a sing
23. or injury by following all precautions for lifting heavy objects gt Ensure that the load is as close to your body as possible gt Ensure that you can cope with the weight of your load CAUTION Routing of the condensation tubing Proper routing of the condensation tubing is critical for correct condensate drainage gt Do not place the multisampler directly on the bench Install Base plate G1328 44121 underneath 1 Next Steps Remove the power cable from the module Open the four screws on cooler cover Slide the sample cooler the half way out Remove power and the signal cable Slide the cooler completely out y OU RAUN Place the sample cooler on the bench Power switch 1 On 2 Off 196 1200 Infinity Series M ultisamplers User M anual Maintenance 9 Install the Sample Cooler Install the Sample Cooler When If the cooler is damaged or defective Tools required Description Screwdriver Pozidriv 1 PT3 Parts required p n Description G4267 60005 Sample Cooler 1 Slide in halfway 2 CAUTION Damage to the cables gt Do not bend or pinch the cables gt Fit in the cooler perfectly 1200 Infinity Series M ultisamplers User Manual 197 9 Maintenance Install the Sample Cooler CAUTION Damage to the sample cooler gt Wait at least 30 min before switching on the compressor of the sample cooler 198 1200 Infinity Series M ultisa
24. position 1 Open the drawer 2 Pull the drawer completely out 1200 Infinity Series M ultisamplers User M anual 189 9 Maintenance Replace the Dummy Drawer 3 Unlatch the drawer Use a screwdriver to press the clamping lever lightly to the left 4 Remove the drawer from the rail guide 190 1200 Infinity Series M ultisamplers User Manual Maintenance 9 5 Grab in the recession below the dummy drawer front panel 1 and lift the left side 2 6 Remove the dummy drawer At this stage remove all other dummies that will be replaced by hotel drawers 1200 Infinity Series M ultisamplers User Manual 191 9 Maintenance Replace the Dummy Drawer 7 Place the new drawer horizontally into the sample hotel Check that the drawer matches the middle bracket of the sample hotel a AUI A 8 Push until the complete drawer locks in place NOTE Take care that the clamping lever locks Always fill sample hotel completely no empty drawer slots Otherwise the drawers can t be configured in the software 9 Configure the hotel drawers in the controller software see the Online Help of the software for details Configuration of the Hotel Drawers The configuration of your drawers is necessary to detect the new drawer configuration for your CDS system When a wrong configuration is detected there will be a mismatch
25. start the maintenance mode and 2 Open the front door select Change needle seat function OR In the Agilent Lab Advisor software select Service amp Diagnostics in the system screen Maintenance Positions gt Change Needle click Start and wait until the needle assembly is in maintenance position 148 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Exchange the Needle Seat 3 Disconnect the seat capillary from the Injection valve 4 Witha flat head screw driver carefully lift out the needle seat 1 Then remove the complete leak tube needle seat assembly from the holder 2 1200 Infinity Series M ultisamplers User Manual 149 9 Maintenance 7 In the Instant Pilot close Change needle seat OR In the Agilent Lab Advisor software Change needle click End and wait until the needle assembly is in the needle park position 8 Close the front door A Eal 9 Perform a pressure test 150 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Replace the Rotor Seal When When poor injection volume reproducibility or when injection valve is leaking Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2394 Hex key 9 64 inch 15 cm long T handle Cleaning tissue and appropriate solvent like isopropanol or methanol Parts required p n Description 5068 0198 Rotor Seal for VIC
26. system The Start Cond step is not always executed Therefore it is recommend to check the box to ensure that at the end of the cleaning procedure the flow path of the sampler is filled with the starting solvent conditions for the next sample For each cleaning step Click the Solvent down arrow and select the solvent to use S1 S2 S3 or switch the step off Specify a duration in seconds in the Time s field M ark the check boxes for Seat Back Flush and or Needle Wash to include these actions If both are selected they are carried out simultaneously if neither is selected the step is ignored equivalent to selecting Off Add a comment in the Comment column if necessary Stoptime Posttime A Multisampler Stoptime can be set For equilibration of the Multisampler a Posttime can be set 84 1200 Infinity Series M ultisamplers User Manual Preparing the module 4 Setting up the Autosampler with Agilent Open Lab ChemStation Module Configuration View The settings are available via menu Instrument gt Instrument Configuration gt Multisampler Configuration Device name based on the module Type ID based on the module product number Some modules may allow changing the type based on hardware firmware This results in a change of features and functions Serial number based on the module Firmware revision based on the module Options lists installed options NOTE Changes in th
27. system Contains fluorinated greenhouse gas refrigerant according to the Kyoto protocol For specifications of refrigerant charge capacity carbon dioxide equivalent CDE and global warming potential GWP see instrument label Confirms that a manufactured product complies with all applicable European Community directives The European Declaration of Conformity is available at http regulations corporate agilent com DoC search htm Manufacturing date Power symbol indicates On Off The apparatus is not completely disconnected from the mains supply when the power switch is in the Off position 1200 Infinity Series M ultisamplers User Manual Appendix 14 Table 19 Symbols Pacemaker A Magnets could affect the functioning of pacemakers and implanted heart defibrillators A pacemaker could switch into test mode and cause illness A heart defibrillator may stop working If you wear these devices keep at least 55 mm distance to magnets Warn others who wear these devices from getting too close to magnets Magnetic field A Magnets produce a far reaching strong magnetic field They could damage TVs and laptops computer hard drives credit and ATM cards data storage media mechanical watches hearing aids and speakers Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields Indicates a pinching or crushing hazard A A Indicates a piercing or cutting hazard WA
28. systems The ERI REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shutdown prepare and so on With the appropriate software the LAN connector may be used to control the module from a computer through a LAN connection This connector is activated and can be configured with the configuration switch With the appropriate software the USB connector may be used to control the module from a computer through a USB connection The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EM C regulations 1200 Infinity Series M ultisamplers User M anual 235 12 Hardware Information Electrical Connections Rear view of the module Configuration switch USB Mini Port LAN ERI CAN Power socket Figure 47 Rear view of multisampler electrical connections and label Information on Instrument Serial Number Serial Number Information 1200 Series and 1290 Infinity The serial number i
29. ultisamplers User Manual Appendix 14 Solvent Information Observe the following recommendations on the use of solvents Brown glass ware can avoid growth of algae e Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid and nitric acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCly O gt gt 2COCly 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropyl ether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF 1200 Infinity Series M ultisamplers User Manual 267 14 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com 268 1200 Infinity Series M ultisamplers Use
30. your site and initiate appropriate actions 50 1200 Infinity Series M ultisamplers User Manual UsingtheModule 3 Install the Sample Cooler Parts required p n Description M ultisampler G7167 60005 Sample cooler Power cord Hardware required Other cables see below and Cable Overview on page 220 Software required OpenLabCDS and or Instant Pilot G4208A with the appropriate revisions see Table 2 on page 35 If the sample cooler is disconnected from the power supply you should wait for at least five minutes before replugging and switching on the compressor again Even under average humidity conditions a significant amount of condensed water gathers every day A suitable container must be provided and emptied regularly in order to avoid overflow 1 Place the Autosampler on the bench 2 Ensure that the power switch on the front of the module is OFF switch stands out 1200 Infinity Series M ultisamplers User Manual 51 3 Using the Module Installing the Sample Cooler 3 Ensure that the power cable is removed from the 4 Open the 4 screws on the rear of the module instrument 52 1200 Infinity Series M ultisamplers User Manual UsingtheModule 3 Installing the Sample Cooler 5 Remove the sample cooler mainframe cover 6 Slide in the sample cooler the halfway Module is partially energized when switched off as long as the power cord is plugged in Repair work at the mod
31. 0 uL to the injection volume of your injector The total volume is then 500 uL or 1500 uL for the Multisampler with a 100 uL analytical head setup Note the delay volume of your Multisampler is extended when using the extended seat capillaries fromthe multi draw kit When calculating the delay volume of the Multisampler you have to double the volume of the extended capillaries The system delay volume due to the Multisampler will increase accordingly Whenever a method is scaled down from a larger column to a smaller column it is important that the method translation makes an allowance for reducing the injection volume in proportion to the volume of the column to maintain the performance of the method This is to keep the volume of the injection at the same percentage volume with respect to the column This is particular important if the injection solvent is stronger more eluotropic than the starting mobile phase and any increase will affect the separation particularly for early running peaks low retention factor In some cases it is the cause of peak distortion and the general rule is to keep the injection solvent the same or weaker than the starting gradient composition This has a bearing on whether or by how much the injection volume can be increased and the user should check for signs of increased dispersion wider or more skewed peaks and reduced peak resolution in trying to increase the injection size If an injection is made in a weak so
32. 11 0 20 uL optional 40 uL in 0 1 uL increments default 0 100 uL optional 500 uL in 0 1 uL increments default lt 0 15 RSD or SD lt 10 nL whatever is greater Up to 1300 bar G7167B 0 2 5cp 1H Drawer up to 8 drawers and 16 positions Shallow well plates MTP 2H Drawer up to 4 drawers and 8 positions MTP deep well plates vials Eppendorf 3H Drawer up to 2 drawers and 4 positions MTP deep well plates vials up to 6 mL Eppendorf lt 10 s using following standard conditions Default draw speed 100 uL min Default eject speed 400 uL min Injection volume luL lt 5s using a Dual needle setup analytical head Pressure range up to 1300 bar M ulti draw modus Injection into needle seat capillary Up to 1300 bar using 40 uL analytical head Up to 1300 bar using 100 uL analytical head M ulti load M easured caffeine M ax pressure for basic instrument M ax 6144 1536 samples 384M TP 96 3072 samples 432 vials 2 mL 1536 samples 60 vials 6 mL 384 vials 1 mL 216 vials 2 mL Using standard Single needle setup Time between 2 injections is not mechanically limited time delay depends on communication speed of software OS or network connections 1200 Infinity Series M ultisamplers User Manual 35 2 Site Requirements and Specifications Table 2 Agilent 1290 Infinity II M ultisampler G7167B Performance Specifications Type Specification Comment Carry Over Multiwash
33. 184 Replace the Dummy Drawer 187 Optional Configurations 187 Installing and Replacing of Drawers Upgrade Drawer Kit 189 Configuration of the Hotel Drawers 192 Remove the Sample Cooler 196 Install the Sample Cooler 197 Replace the M odule Firmware 200 gig Agilent Technologies 129 9 Maintenance Injector Steps This chapter describes the maintenance of the M ultisampler 130 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Introduction to M aintenance Figure 29 on page 131 shows the main user accessible assemblies of the multisampler These parts can be accessed from the front simple repairs and don t require to remove the multisampler from the system stack Peristaltic pump Metering device Dummy drawer Needle assembly Needle seat Injection valve Figure 29 Main user accessible assemblies standard 1200 Infinity Series M ultisamplers User Manual 131 9 Maintenance Introduction to Maintenance SSV piezo pump Flush head Metering device Dummy drawer Needle assembly Needle seat Injection valve Sample container vial tray Sample container wellplate Sample tray Figure 31 Overview of drawer sample tray and sample container 132 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Warnings and Cautions WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by i
34. 2 ft Up to 4600 m 15091 ft Installation category Il Pollution degree 2 For indoor use only 1200 Infinity Series M ultisamplers User Manual CAUTION General hazards and improper disposal Site Requirements and Specifications 2 Improper disposal of the media and components used pollutes the environment gt The breakdown of the sample cooler unit must be carried out by specialist refrigeration company gt All media must be disposed of in accordance with national and local regulations gt Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance Table5 Performance Specifications Agilent 1290 Sample Cooler Type Specifications Operating principle Temperature range Temperature settable Temperature accuracy lt 25 C lt 50 rH Temperature Stability High performance low energy consumption micro compressor based cooler with ozone friendly R134A coolant 42 g user upgradable from 4 C to ambient from 4 40 C in 1 increments 2 C to 6 C at a setpoint of 4 C 2 C ata setpoint of 4 C 1200 Infinity Series M ultisamplers User Manual 41 2 42 Site Requirements and Specifications Physical Specifications of the Sample Cooler 1200 Infinity Series M ultisamplers User Manual This chapter explains the essential operational parameters of the module 1200 Infinity Series Multisamplers User M anual 3 Using th
35. 5 1717 Metering seal 40 uL for 40 uL analytical head 1 0905 1719 Metering seal 100 uL for 100 uL analytical head 1 5067 5920 Piston ceramic If previous piston is scratched Preparations Removing the metering seal see Remove the Metering Seal on page 157 162 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Install the M etering Seal 1 Install the new metering seal using the plastic side of the 2 Reassemble the support ring insert tool Press it firmly into position Avoid any offset angle as it might deform the seal 1200 Infinity Series M ultisamplers User Manual 163 9 Maintenance Install the Metering Seal a Tighten the three screws fingerthight then b Tighten the screws alittle at a time to keep the support ring surface parallel important to the surface of the analytical head 3 Make sure to comply to the following order of actions 4 Use the twist and lock bayonet mechanims to reassemble the analytical head assembly Push the two parts together to couple the head body with the analytical head Once the pin reaches the bottom of the slot one or both parts are rotated so that the pin slides along the horizontal arm of the L until it reaches the serif The spring then pushes the male connector up into the serif to keep the pin locked into place 164 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Install the M etering Seal 5 Press
36. Functions and Calibration Arm Position The home position of the multisampler ensures a better access to the workspace When transporting the module it is highly recommended to use the Instrument Control gt Park Position command in order to place the Sample Handler in a position for safe transport Special Commands Clear Emor Sampler Reset Forced Cold Start Park Postion Figure 27 Park Position Button Change Metering Device When removing the metering device is necessary by exchanging the metering seal for instance the metering drive needs to be moved to a position at the far back in order to prevent seal and or piston damage Maintenance Positions Task Selection Qhange Rotor Seal No Stat gt gt Change Loop No Change Meters Dene Ye LD Press the Start gt gt button in order to begin Procedre Change Seat No Change the metering device Leave the service mode Qhange Neede No Figure 28 Change Metering Device 1200 Infinity Series M ultisamplers User Manual Test Functions and Calibration 8 Injector Steps Injector Steps Each movement of the sampling sequence can be done under manual control This is useful during troubleshooting where close observation of each of the sampling steps is required to confirm a specific failure mode or verify successful completion of a repair Each injector step command actually consists of a series of individual commands that move the multisampler c
37. I Injection Valve PAEK CAUTION Reduced life time of the injection valve Component cleanliness is crucial for the life time of the injection valve gt Replace the rotor seal in a clean environment 1200 Infinity Series M ultisamplers User M anual 151 9 Maintenance Replace the Rotor Seal 1 Open the front door 2 Remove all capillaries from the injection valve with a 1 4 inch wrench NOTE Remember the correct plumbing 152 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Replace the Rotor Seal 3 Use a 9 64 inch hex driver to unscrew the two socket screws which hold the stator head in place CAUTION Damage to the stator head The polished sealing surface of the stator head contains six ports that access handling can easily damage gt Avoid touching the polished surface of the stator head gt Never place the polished surface on a hard surface 4 Carefully remove the stator head To ensure that the sealing surface of the stator head is not damaged place it on its outer face 1200 Infinity Series M ultisamplers User Manual 153 9 Maintenance Replace the Rotor Seal 5 Remove the rotor seal Remove the rotor seal with a small tool gently pry the rotor seal away from the drive Examine the rotor sealing surface for scratches and nicks e If scratches are visible the rotor seal must be replaced e Ifno scratches are visible clean al
38. Infinity Series M ultisamplers User Manual Appendix 14 Before Applying Power WARNING Wrong voltage range frequency or cabling Personal injury or damage to the instrument gt Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations gt Make all connections to the unit before applying power Note the instrument s external markings described under Symbols on page 260 Ground the Instrument WARNING Missing electrical ground Electrical shock gt If your product is provided with a grounding type power plug the instrument chassis and cover must be connected to an electrical ground to minimize shock hazard gt The ground pin must be firmly connected to an electrical ground safety ground terminal at the power outlet Any interruption of the protective grounding conductor or disconnection of the protective earth terminal will cause a potential shock hazard that could result in personal injury 1200 Infinity Series M ultisamplers User Manual 257 14 Appendix Do Not Operate in an Explosive Atmosphere WARNING Presence of flammable gases or fumes Explosion hazard gt Do not operate the instrument in the presence of flammable gases or fumes Do Not Remove the I
39. Install the Sample Cooler 197 Replace the Module Firmware 200 10 Parts for Maintenance and Upgrade or Options Overview of Maintenance Parts 202 Sampler Main Assemblies 203 Hotel Drawer 205 Analytical Head Assembly 40 uL 206 Analytical Head Assembly 100 uL 207 Analytical Head Assembly 900 uL 208 Flush Head Assembly 500 uL 209 Injection Valve Assembly 211 Injection Valve with Actuator 213 Needle Port Assembly 214 Door Assy 215 Accessory Kit 216 Tubing Kit Sampler Standard 217 Sample Cooler 218 11 Identifying Cables 219 Cable Overview 220 Analog Cables 222 Remote Cables 224 CAN LAN Cables 228 Agilent Module to PC 229 USB 230 1200 Infinity Series M ultisamplers User M anual Contents 201 Contents 12 Hardware Information 231 Firmware Description 232 Electrical Connections 235 Interfaces 238 Setting the 6 bit Configuration Switch 246 Instrument Layout 248 Early Maintenance Feedback 249 13 LAN Configuration 251 Setting up the module ina LAN environment 252 Connecting the module via LAN 253 14 Appendix 255 General Safety Information 256 Waste Electrical and Electronic Equipment Directive 262 Refrigerant 263 Radio Interference 265 SoundEmission 266 Solvent Information 267 Agilent Technologies on Internet 268 8 1200 Infinity Series M ultisamplers User Manual 1200 Infinity Series M ultisamplers User M anual 1 Introduction Product Description G7167B 10 Product Description G7167A 11 Fe
40. Instrument 258 In Case of Damage 258 Solvents 259 Symbols 260 Waste Electrical and Electronic Equipment Directive 262 Refrigerant 263 Radio Interference 265 Sound Emission 266 Solvent Information 267 Agilent Technologies on Internet 268 This chapter provides addition information on safety legal and web ot Agilent Technologies 255 14 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards General Do not use this product in any manner not specified by the manufacturer The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions 256 1200
41. LC applications 1200 Infinity Series M ultisamplers User Manual Preparing the module 4 Recommended M ats and Vials Table7 Recommended plates and closing mat Description Part Number Rows Columns Plate height Volume uL Package 384A gilent 5042 1388 16 24 14 4 80 30 384Corning No Agilent PN 16 24 14 4 80 384Nunc No Agilent PN 16 24 14 4 80 96 well plate 0 5 ml PP pack of 10 8 12 143 500 10 5042 1386 120 96 well plate 0 5 ml PP pack of 120 5042 1385 96A gilent conical 5042 8502 8 12 173 150 25 96CappedA gilent 5065 4402 8 12 47 1 300 1 96Corning No Agilent PN 8 12 143 300 96CorningV No Agilent PN 8 12 143 300 96DeepAgilent31mm 5042 6454 8 12 315 1000 50 96DeepN unc31mm No Agilent PN 8 12 31 5 1000 96DeepRitter41mm No Agilent PN 8 12 41 2 800 96Greiner No Agilent PN 8 12 14 3 300 96GreinerV No Agilent PN 8 12 14 3 250 96Nunc No Agilent PN 8 12 14 3 400 Closing mat for all 96 Agilent plates 8 12 50 5042 1389 Recommended Vial Plates p n Description G2255 68700 Vial plate for 54 x 2 mL vials 6 pk 1200 Infinity Series M ultisamplers User Manual 71 4 Preparing the module Recommended M ats and Vials p n Description 5022 6539 Vial plate for 15 x 6 mL vials 1 pk 5022 6538 Vial plate for 27 Eppendorf tubes 1 pk NOTE Agilent Technologies recommends to use preslit septa Bottom sensing is a feature to detect the depth of vials or plates via the software If the bot
42. N 192 168 254 11 For specific LAN modes switches 4 5 must be set as required For boot resident cold start modes switches 1 2 or 6 must be UP Configuration switch Figure 50 Location of Configuration switch example shows a G7114A B VWD Table 18 6 bit Configuration Switch ee moe n n Switch 1 Switch 2 Switch 3 Switch 4 Switch 5 Switch 6 com 0 na na LAN Init Mode na Use Default IP Address 0 0 0 0 0 Use Stored IP Address 0 0 0 1 0 Use DHCP to request IP Address 0 0 1 0 0 246 1200 Infinity Series M ultisamplers User Manual Table 18 6 bit Configuration Switch Mode Function Setting Hardware Information 12 Test 1 Boot Main System Keep Data Boot Resident System Keep Data Boot Main System Revert to Default Data Boot Resident System Revert to Default Data System 0 1 0 0 0 0 n a 0 0 0 n a 0 0 0 ColdStart 0 0 1 en from non volatile memory 3 Default IP Address is 192 168 254 11 4 Host Name will be the MAC address 1200 Infinity Series M ultisamplers User Manual not assigned Always keep these switches on position 0 off When selecting mode COM settings are stored to non volatile memory When selecting mode TEST COM settings are tak 247 12 Hardware Information Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC
43. N cable Agilent module to module 1 m S Figure 43 Accessory kit standard 216 1200 Infinity Series M ultisamplers User M anual Parts for Maintenance and Upgrade or Options 10 Tubing Kit Sampler Standard Tubing Kit Sampler Standard Item p n 64267 60061 5042 9974 5500 1155 0890 1760 5042 6422 0100 1708 0100 1700 0100 1846 ry DoD UO A WD N Fe N Fa Description Tubing Kit Sampler Standard contains Tubing Flex 1 5 m Tube Connector 90 degree ID 6 4 Tubing Flexible 1 ea 1 meter Tubing connector 1 mm o d Nut 1 8 PPS FERRULE AY 18IN UNION TEFZEL Figure 44 Tubing kit sampler standard 1200 Infinity Series M ultisamplers User M anual 217 10 Parts for Maintenance and Upgrade or Options Sample Cooler Sample Cooler ltem p n 1 G4267 60005 G4267 81015 G4267 81014 2110 1519 Description Sample Cooler Cable Power Sample Cooler not shown Cable Ribbon Sample Cooler not shown Fuse 3 50 A125 V not shown Figure 45 Sample cooler 218 1200 Infinity Series M ultisamplers User M anual This chapter provides information on cables used with the modules 1200 Infinity Series Multisamplers User M anual 11 Identifying Cables Cable Overview 220 Analog Cables 222 Remote Cables 224 CAN LAN Cables 228 Agilent Moduleto PC 229 USB 230 gg Agilent Technologies 219 11 Identifying Cables Cable Overview Never use cables other than the ones supplied by Agi
44. RNING alia alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 1200 Infinity Series M ultisamplers User M anual 261 14 Appendix Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex this product is classed as a Monitoring and Control Instrumentation product Do not dispose of in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 262 1200 Infinity Series M ultisamplers User Manual Appendix 14 Refrigerant The refrigerant HFKW 134a is used only in the Agilent Infinity II Sample Cooler Ta
45. ad 209 frequency range 34 G general error messages 111 guide coding 73 color 73 269 Index H hotel drawer configure in control software 193 configure in Lab Advisor 195 hotel drawer 205 humidity 34 indicator drawerstatus 47 injection valve actuator 213 installation bench space 32 sample cooler 51 upgrade drawer 189 installing sample loop flex 184 instrument layout 248 interfaces 106 Infinity Il 238 internet 268 L LAN cable 228 configuration 251 environment 252 setup 252 leak sensoropen 114 leak sensor short 113 leak 116 line frequency 34 line voltage 34 lost CAN partner 113 270 M maintenance feedback 249 overview 202 135 replacing firmware 200 mats 71 message remote timeout 112 metering seal install 162 remove 157 method parameter settings 82 module firmw are replace 200 module configuration 85 overview 14 multisampler transport 58 N needle assembly installation 144 remove 140 needle port assembly 214 needle seat exchange 148 non operating altitude 34 non operating temperature 34 0 operating Altitude 34 operating temperature 34 optimization achieving higher resolution 98 achieving higher sensitivity 101 columnuse 101 overview cable 220 module 14 P packaging damaged 50 physical specifications 34 power consideration 30 powerconsumption 34 powercords 31 principle 16 product description 11 R remote cables 224 removing sample l
46. afety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 134 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Overview of Maintenance It is necessary to perform periodic inspection of this instrument to ensure its safe use It is possible to have these periodic inspections performed by Agilent service representatives on a contractual basis For information regarding the maintenance inspection contract contact your Agilent representative The following pages describe the maintenance simple repairs of the module that can be carried out without opening the main cover Table 12 Overview of maintenance Procedure Typical interval minimum Notes Change 60000 needle into seat needle needle seat Change peristaltic 3000 min on time pump cartridge Change rotor seal 30000 injections 1200 Infinity Series M ultisamplers User Manual 135 9 Maintenance Clean the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connec
47. al 211 10 Parts for Maintenance and Upgrade or Options Injection Valve Assembly 3 a 4 w Figure 39 Injection valve assembly VICI 212 1200 Infinity Series M ultisamplers User M anual Parts for Maintenance and Upgrade or Options 10 Injection Valve with Actuator Injection Valve with Actuator Item p n Description 1 5067 4232 2pos 6port Injection Valve VICI 2 5043 0291 Lock Nut 3 5188 8030 Tag Reader 4 5067 4162 Direct Actuator 50 Assembly Figure 40 Injection valve with actuator 1200 Infinity Series M ultisamplers User Manual 213 10 Parts for Maintenance and Upgrade or Options Needle Port Assembly Needle Port Assembly Item p n Description 1 G4267 60044 Needle Port Assembly Station 2 G4267 40045 Needle port Adapter Figure 41 Needle port assembly 214 1200 Infinity Series M ultisamplers User Manual Parts for Maintenance and Upgrade or Options 10 Door Assy Door Assy Item p n Description 5067 5415 Door Assy 1 5021 1879 Permanent M agnet 2 64267 68713 Pressure Spring 3 5067 5412 2x Hinge Universal Figure 42 Door assy 1200 Infinity Series M ultisamplers User Manual 215 10 Parts for Maintenance and Upgrade or Options Accessory Kit Accessory Kit Item p n Description G4267 68705 Accessory Kit 1 64220 60007 Bottle Head Assembly 2 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 3 5500 1157 Capillary ST 0 12 mmx500 mm 4 5043 1013 Tubing Clip 5 5181 1519 CA
48. al for correct condensate drainage gt Do not place the multisampler directly on the bench Install Base plate G1328 44121 underneath CAUTION Damage through condensation If the condensation tube is located in liquid the condensed water cannot flow out of the tube and the outlet is blocked Any further condensation will then remain in the instrument This may damage the instruments electronics gt Make sure the condensation tube is always above the liquid level in the vessel gt Make sure the waste container is not sealed gt Ensure the drain tube has no loops and leads directly into the waste bottle Heavy weight The module is heavy gt 22 kg gt 46 lbs gt Carry the module at least with 2 people gt Avoid back strain or injury by following all precautions for lifting heavy objects gt Ensure that the load is as close to your body as possible gt Ensure that you can cope with the weight of your load 10 Lift the multisampler with the sample cooler installed into the LC stack 11 Use a bubble level to check the leveling of the M ultisampler The sample cooler should operated in a proper horizontal position 1200 Infinity Series M ultisamplers User Manual 55 3 Using the Module Installing the Sample Cooler 12 Install the condensate tubing on the drain outlet of the sample cooler and guide the condensation tubing out to the central waste Do not kink the drain tubing
49. amplers User M anual 10 Parts for Maintenance and Upgrade or Options Overview of Maintenance Parts 202 Sampler Main Assemblies 203 Hotel Drawer 205 Analytical Head Assembly 40 uL 206 Analytical Head Assembly 100 uL 207 Analytical Head Assembly 900 uL 208 Flush Head Assembly 500 uL 209 Injection Valve Assembly 211 Injection Valve with Actuator 213 Needle PortAssembly 214 DoorAssy 215 Accessory Kit 216 Tubing Kit Sampler Standard 217 Sample Cooler 218 This chapter provides information on parts material required for the module ee Agilent Technologies 201 10 Parts for Maintenance and Upgrade or Options Overview of Maintenance Parts p n Description 0905 1717 Metering seal 40 uL 0905 1719 Metering seal 100 uL 5067 5918 Metering seal 500 uL 5068 0007 Injection valve rotor seal 64267 87201 Needle Assembly G4267 87012 High Pressure Needle Seat 0 12 mm PEEK 64267 60300 Sample Loop Flex 20 uL right red coded 64267 60400 Sample Loop Flex 40 uL right green coded G4267 60500 Sample Loop Flex 100 uL right blue coded 5065 4445 Peristaltic pump with Pharmed tubing 202 1200 Infinity Series M ultisamplers User Manual Parts for Maintenance and Upgrade or Options 10 Sampler Main Assemblies p n 64267 60002 G7167 60020 G7167 60021 G7167 60022 64267 60001 5067 4232 64267 87201 64267 60044 64267 60045 64267 87012 64267 60300 64267 60400 64267 60500 64267 60050 Description Hotel Assembly Drawer 2H D
50. amples of incorrect fittings Ferrule cannot seat properly Mixing chamber iii X If dimension X is too long If dimension X is too short leaks will occur a dead volume or mixing chamber will occur Figure 13 Examples of incorrect fittings 76 1200 Infinity Series M ultisamplers User M anual Preparing the module 4 Setting up the Autosampler with Agilent Open Lab ChemStation Setting up the Autosampler with Agilent Open Lab ChemStation The setup of the Multisampler is shown with the Agilent OpenLab ChemStation C 01 06 Depending on the controller e g Agilent Instant Pilot OpenLab EZChrom Masshunter the screens look different This section describes the autosampler settings only For information on the Agilent OpenLab ChemStation or other 1290 Infinity modules refer to the corresponding documentation Male een Sich em O 2 ower ih heh i ofS 3 EESE Y Meee aren bs ened ome 3 roe awe j __ oms i Figure 14 ChemStation M ethod and Run Control After successful load of the OpenLab ChemStation you should see the module as an active item in the graphical user interface GUD 1200 Infinity Series M ultisamplers User Manual 77 4 Preparing the module Setting up the Autosampler with Agilent Open Lab ChemStation 78 1200 Infinity Series M ultisamplers User Manual Preparing the module 4 Setting up the Autosampler with Agilent Open Lab ChemStation Table8 The Autosampler U
51. an impact on the decision to use ADVR or not At 0 2 ml min the delay time saved is 21 seconds while at 1 0 ml min it is 4 seconds The ADVR function is unlikely to be suitable for applications involving compounds which are known to cause carry over problems The best solution to reduce the delay volume is to install the 40 uL Analytical Head and the 20 uL Loop To get the best results it is also recommended to order the Low dispersion heat exchanger and the micro flow cell for UV This will reduce the the delay volume by 120 uL 1200 Infinity Series M ultisamplers User Manual 93 5 Optimizing Performance How to Achieve Higher Injection Volumes 94 The standard configuration of the Multisampler can inject a maximum volume of 20 uL with the standard loop capillary To increase the injection volume the Multidraw upgrade kit G4216 68711 can be installed With this kit you can add a maximum of 80 uL to the injection volume of your injector The total volume for the standard Multisampler is then 100 uL or 120 uL depending on the loop size with 40 uL analytical head installed For higher injection volume you can choose between further options This requires additional hardware modifications One way to increase the injection volume is to change the analytical head volume There are a 100 uL and 900 uL analytical heads available Additional you can install the Multidraw kit G7167 68711 With the kit you can add a maximum of 400 uL or 140
52. any single system Seamless automation Internal robotics move microtiter plates and other sample containers from the sample hotel to the central workspace for sample processing steps and injections Dual needle injection By running samples alternately through one or the other injection path you can reduce cycle times to mere seconds virtually eliminating conventional wait times whether for large volume loadings or flushing procedures Scalable injection volumes The Agilent dual needle setup enhances flexibility by providing two differently optimized injectors in a single instrument You can for example optimize one path for large volume injections and the other for low delay volumes Ultralow carryover The 1260 Infinity Multisampler has a low carryover and a multi wash capability cleansing all relevant injection parts between runs This integrated feature flushes the injection needle outside with three solvents and uses seat backflush procedures to reduce carryover to less than 9 ppm Efficient temperature control For temperature sensitive samples add Agilent s compressor based cooling system It maintains temperature control on all vials and plates inserted into the 1260 Infinity Multisampler Instant information Lights on each drawer tell you about loading status current activity and accessibility 1200 Infinity Series M ultisamplers User Manual 13 1 Introduction Overview of t
53. aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated acids and bases For normal phase applications the maximum pressure should be limited to 200 bar Tantalum Ta Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide It can be corroded by strong bases e g hydroxide solutions gt 10 diethylamine It is not recommended for the use with fluoric acid and fluorides Stainless Steel ST Stainless steel is inert against many common solvents It is stable in the presence of acids and bases in a pH range of 1 to 12 5 It can be corroded by acids below pH 2 3 It can also corrode in following solvents Solutions of alkali halides their respective acids for example lithium iodide potassium chloride and so on and aqueous solutions of halogens High concentrations of inorganic acids like nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCly Oy 2 COCly 2 HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol 1200 Infinity Series M ultisamplers User Manual Pre
54. at can lead to slightly increased dispersion and possibly a small selectivity change both of which could be seen as a reduction in resolution The latter case might be offset by reducing the temperature of the thermostat by a few degrees and again experiment will reveal the answer The van Deemter curve shows that the optimum flow rate through an STM column is higher than for larger particles and is fairly flat as the flow rate increases Typical close to optimum flow rates for STM columns are 2 ml min for 4 6 mm i d and 0 4 ml min for 2 1 mm i d columns In isocratic separations increasing the retention factor k results in better resolution because the solute is retained longer In gradient separations the retention is described by k in the following equation ps _lo F 100 A B V S where k mean k value tg time length of gradient or segment of gradient min 1200 Infinity Series M ultisamplers User Manual 99 5 Optimizing Performance F flow ml min Vin column delay volume A B change in fraction of solvent B during the gradient e S constant ca 4 5 for small molecules This shows that k and hence resolution can be increased by having a shallower gradient 2 to 5 min change is a guideline higher flow rate and a smaller volume column This equation also shows how to speed up an existing gradient if the flow is doubled but the gradient time is halved k remains constan
55. ate of the resident system can be done in the main system only Main and resident firmware must be from the same set 1200 Infinity Series M ultisamplers User Manual 233 12 Hardware Information 234 Main FW update Resident System Main System Resident FW Update Figure 46 Firmware Update M echanism Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A XX xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whi d 69761 1200 Infinity Series M ultisamplers User Manual Hardware Information 12 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization One analog output provides signals for integrators or data handling
56. atures G7167B 12 Features G7167A 13 Overview ofthe Module 14 Multisampler Principle 16 System Overview 22 Leak and Waste Handling 22 Leak and Waste Handling in a Mixed Configuration 26 This chapter gives an introduction to the M ultisampler ott Agilent Technologies 1 Introduction Product Description G7167B Product Description G7167B The Agilent 1290 Infinity II Multisampler can handle both vials and microtiter plates with ease and efficiency up to 1300 bar system pressure optimized on chromatographic performance In fact this compact module has the capacity to house up to 6144 samples all inside the Agilent stack footprint and the robotics to smoothly inject each into the chromatograph in turn With Agilent s unique dual needle design cycle time is just 5 seconds With the multi wash capability you can reduce carryover to less than 9 parts per million Status indicator Needle assy Hotel drawer Leak drain Power switch Figurel Overview of the M ultisampler 10 1200 Infinity Series M ultisamplers User Manual Introduction 1 Product Description G7167A Product Description G7167A The Agilent 1260 Infinity Multisampler can handle both vials and microtiter plates with ease and efficiency up to 600 bar system pressure optimized on high flexibility This compact module can house up to 6144 samples all inside the Agilent stack footprint and the robotics to inject each into the chromatograph
57. ble 20 Physical properties of refrigerant HFKW 134a Molecular weight 102 Critical temperature 101 1 C Critical pressure 40 6 bar Boiling point 26 5 C 1200 Infinity Series M ultisamplers User Manual 263 14 Appendix Refrigerant Refrigerant HFKW 134a is known as a Safe refrigerant however accidents can occur if it is handled incorrectly For this reason the following instructions must be observed gt Avoid contact with liquid refrigerant HFKW 134a At atmospheric pressure HFKW 134a evaporates at approximately 26 C and causes frost bite gt After skin contact rinse the affected area with water gt After eye contact rinse the eye s with plenty of water for at least 15 minutes and consult a doctor gt HFKW 134a must not be allowed to escape in enclosed areas Although HFKW 134a is not toxic there is a danger of suffocation as gaseous refrigerant is heavier than air gt Please observe the following first aid instructions After inhalation move the affected person to fresh air keep him warm and allow him to rest If necessary he should be supplied with oxygen If he has stopped breathing or is breathing erratically he should be given artificial respiration In the case of cardiac arrest carry out heart massage Send for a doctor immediately gt Moreover it must be noted that HFKW 134a must always be extracted from the system and collected It must never be discharged into the atmosphere on environmental
58. bstances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet gt Ground the waste container gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly gt Do not use solvents with an auto ignition temperature below 200 C 392 F 62 Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets 1200 Infinity Series M ultisamplers User Manual Preparing the module 4 Leak and Waste
59. cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 1200 Infinity Series M ultisamplers User Manual 115 7 116 Error Information Leak Probable cause Suggested actions 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 1200 Infinity Series M ultisamplers User Manual 1200 Infinity Series Multisamplers User M anual 8 Test Functions and Calibration Introduction 118 System Pressure Test 119 System Pressure Test Evaluation 121 Auto Referencing 122 Maintenance Positions 124 Change Needle Assembly 124 Change Sample Loop Capillary 125 ArmPosition 126 Change Metering Device 126 Injector Steps 127 This chapter describes the built in test functions ee Agilent Technologies 117 8 Test Functions and Calibration Introduction All tests are des
60. ce Replace the Flushhead Seal 7 Remove the piston from the head body 9 Reassemble the flush head and the head body without piston 8 Carefully remove the metering seal from the tip of the piston Seal 10 Carefully insert the piston with the new metering seal into the flush head assembly Seal 174 1200 Infinity Series M ultisamplers User Manual Maintenance 9 eplace the Flushhead Seal 11 Reinstall the flush head to the actuator housing we Eal TTT 1200 Infinity Series M ultisamplers User Manual 175 9 Maintenance Replace the Injection Valve Replace the Injection Valve When Add new injection valve or replace defective injection valve Tools required Description Wrench 9 64 Parts required p n Description 1 5067 4232 2pos 6port Injection Valve VICI 1300 bar G7167B OR 1 5067 4230 Injection Valve Idex 600 bar G7167A Preparations Switch off the power of the M ultisampler 1 Disconnect the capillaries 2 Turn the spanner nut counter clockwise until the injection valve head detaches from the hydraulic box Do not use wrenches on the spanner nut 176 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Replace the Injection Valve 3 Remove the spanner nut from the injection valve head 4 Take the replacment injection valve head and insert it into the open actuator slot of the hyd
61. ce should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C 1200 Infinity Series M ultisamplers User Manual 239 12 Hardware Information There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table 16 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI 240 1200 Infinity Series M ultisamplers User M anual Hardware Information 12 Instrument PC DCD 1 1 DCD RX 2 2 RX TX 3 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 7 RTS CTS 8 8 CTS RI 9 9 RI DB9 D89 DB9 DB9 Male Female Female Male Figure 48 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board APG Remote The APG Remote connector may be used in combination with other analytical instrument
62. ce the carry over you have two different possibilities First the external needle wash In the Standard configuration the needle flush station is equipped with a peristaltic pump to wash the outside of the needle This reduces the already low carry over for very sensitive analysis The bottle containing the mobile phase for the wash procedure will be located in the solvent bottle cabinet Produced waste during this operation is channeled safely away through a waste drain In the Multi Wash configuration the external needle wash will be done by a micro piezo pump combined with a solvent selection valve where you can select between three different solvents If this is not sufficient to reduce the carry over there is an additional and perfect way to achieve the lowest carry over in the Multi Wash configuration by using the intergrated flush 1200 Infinity Series M ultisamplers User Manual Introduction 1 pump This high pressure pump can also select between three different solvents and it is capable of reducing the carry over to a minimum by using the seat backflushing The flush pump outlet capillary is connected to the port 4 of the Multisampler s injection valve which normally holds the waste line If the Multisampler is in bypass mode the flush pump connects to the needle seat and can flush backwards through the needle seat into the waste line attached to the needle seat outlet port The six port only 5 ports are used injection valve unit is d
63. cribed based on the Agilent Lab Advisor Software B 02 06 or above Other user interfaces may not provide any test or just a few For details on the use of the interface refer to the interface documentation Table 11 Interfaces and available test functions Interface Comment Available Function Agilent Lab Advisor All tests are available System Pressure test e Maintenance Drawer Detection Auto Referencing Adding of pressure to chromatographic signals possible Agilent ChemStation No tests available Drawer Detection Auto Referencing Adding of pressure to Temperature mainboard chromatographic signals e Pressure Pressure ripple possible For details on the use of the interface refer to the interface documentation 118 1200 Infinity Series M ultisamplers User Manual Test Functions and Calibration 8 System Pressure Test The test determines the leak rate of the system between pump outlet valves and a blank nut The blank nut can be positioned at different locations in the system before the flow cell to determine and verify the leak rate of individual modules and components The test allows for setting the pressure at which the test is performed The leak rate of high pressure parts is not always a linear function and therefore it is recommended to perform the test at a pressure that corresponds to the normal operating pressure of the system When Incase of a suspected leak e To verify successful execution of main
64. ctable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EM F Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EM F Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1200 Infinity Series M ultisamplers User Manual 249 12 Hardware Information Early Maintenance Feedback 250 1200 Infinity Series M ultisamplers User Manual
65. ctice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1200 Infinity Series M ultisamplers User Manual In This Guide In This Guide This manual covers the following modules Agilent 1290 Infinity II Multisampler G7167B Agilent 1260 Infinity Multisampler G7167A 1 Introduction This chapter gives an introduction to the Multisampler 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Using the Module This chapter explains the essential operational parameters of the module 4 Preparing the module This chapter explains the operational parameters of the module 5 Optimizing Performance This chapter gives hints on how to optimize the performance or use additional devices 6 Troubleshooting and Diagnostics This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces 7 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 1200 Infinity Series M ultisamplers User Manual 3 In This Guide 8 Test Functions and Calibration This chapter describes the built in test functions 9
66. cts When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 168 The peristaltic pump cartridge is a replaceable unit The tubing inside the pump is not replaceable 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Replace the Peristaltic Pump Cartridge 1 Open the front door 2 Press the two clips on the front of the peristaltic pump cartridge 3 Pull the cartridge forward off the motor shaft 4 Disconnect the tubing coupler leading to the wash port ay and the tubing coupler coming from the solvent bottle 1200 Infinity Series M ultisamplers User Manual 169 9 Maintenance Replace the Peristaltic Pump Cartridge 5 Push the new cartridge onto the motor shaft until the 6 Connect the wash port tubing to the upper tubing of the clips click into place new cartridge use sand paper to get a good grip on the tubing 170 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Replace the Peristaltic Pump Cartridge 7 Connect the inlet filter of the solvent bottle again Use the syringe to draw enough solvent for compl
67. cument are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 J une 1995 oras a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 J une 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 J une 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 J une 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure pra
68. d Sample Loop Flex 100 uL right blue coded Preparations Finish any pending acquisition job and return any plate on the workspace back to the hotel WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Do not open the safety lock of the needle assembly gt Be careful working at the z robot gt Wear safety goggles when removing the needle assembly OR 1 Inthe Instant Pilot start the maintenance mode and 2 Open the front door select Change needle seat function In the Agilent Lab Advisor software select Service amp Diagnostics in the system screen Tools Maintenance Positions gt Change Needle Loop click Start and wait until the needle assembly is in maintenance position 180 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Removing the Sample Loop Flex 3 The needle assembly is still connected to the loop capillary Use a 1 4 inch wrench to loosen the fitting of the loop capillary connected to the analytical head Damage of the loop The loop shape may be damaged if the loop is stretched or bent too far gt Avoid to change the loop shape gt Do not pull or bend the loop too far Sharp needle Uncovered needles may cause injuries gt Do not unlock the saftey lock of needle assembly 4 Remove the needle assembly by slightly pulling the needle cartridge 1200 Infinity Series M ult
69. e Module Magnets 44 Turn on off 45 Status indicators 46 Drawer Status Indicator 47 Insert vial trays wellplates 48 Remove vial trays wellplates 49 Installing the Sample Cooler 50 Damaged Packaging 50 Install the Sample Cooler 51 Transporting the Multisampler with a Sample Cooler Installed 58 ott Agilent Technologies 43 Using the Module Magnets 3 Magnets 1200 Infinity Series M ultisamplers User Manual Using the Module Turn on off Turn on off Power switch 1 On 2 Off 1200 Infinity Series M ultisamplers User Manual 45 3 Using the Module Status indicators Status indicators 1 The module status indicator indicates one of six possible module conditions Status indicators 1 Idle 2 Run mode 3 Not ready Waiting for a specific pre run condition to be reached or completed 4 Error mode interrupts the analysis and requires attention for example a leak or defective internal components 5 Resident mode blinking for example during update of main firmware 6 Bootloader mode fast blinking Try to re boot the module or try a cold start Then try a firmware update 46 1200 Infinity Series M ultisamplers User Manual UsingtheModule 3 Drawer Status Indicator Drawer Status Indicator The module status indicator indicates one of three possible module conditions When the status indicator is OFF no sample containers are loaded e When the upper
70. e between 2 injections is not mechanically limited time delay depends on communication speed of software OS or network connections Using the following conditions e Column Agilent ZORBAX SB C18 2 1x 50 mm1 8 mm 827700 902 Mobile Phase A 0 1 TFA in water B 0 1 TFA in Acetonitrile Isocratic B 35 Flow rate 0 5 mL min Temperature 25 C e Wavelength 257 nm Sample 1200 ng uL Chlorhexidine dissolved with mobile phase A 1 uL injected and measured on G4212A DAD e Wash solution H20 with 0 1 TFA 5s A 02 01 or above A 02 02 supports Sample evaluation Entry Ul M assHunter QQQ B 07 00 SP1 or above MassHunter QTOF B 05 01 SP3 or above Lab Advisor B 02 05 or above ICF for 3rd party SW control A 02 01 or above LC and CE Drivers A 02 10 or above 38 1200 Infinity Series M ultisamplers User Manual Site Requirements and Specifications 2 Table 3 Agilent 1260 Infinity M ultisampler G7167A Performance Specifications Type Specification Comment Local Control Communications Safety and maintenance GLP features Housing Metering device Agilent Instant Pilot G4208A B 02 17 or above currently not supported official release 2015 Controller area network CAN Local Area Network LAN ERI ready start stop and shut down signals Extensive support for troubleshooting and maintenance is provided by the Instant Pilot Agilent Lab Advisor and the Chromatography Data System Sa
71. e materials exhibit excellent robustness under typical HPLC conditions For any special conditions please consult the material information section or contact Agilent 1200 Infinity Series M ultisamplers User Manual Preparing the module 4 Disclaimer Subsequent data were collected from external resources and are meant as a reference Agilent cannot guarantee the correctness and completeness of such information Data is based on compatibility libraries which are not specific for estimating the long term life time under specific but highly variable conditions of UHPLC systems solvents solvent mixtures and samples Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Apart from pure chemical corrosion other effects like electro corrosion electrostatic charging especially for non conductive organic solvents swelling of polymer parts etc need to be considered Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually accelerates at higher temperatures If in doubt please consult technical literature on chemical compatibility of materials PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance mechanical and thermal stability PEEK is therefore the material of choice for UHPLC and biochemical instrumentation It is stable in a pH range betw
72. e sampler configuration can only be done in the online view of the CDS system see Table 8 on page 79 1200 Infinity Series Multisamplers User M anual 85 4 Preparing the module Setting up the Autosampler with Agilent Open Lab ChemStation 86 1200 Infinity Series M ultisamplers User Manual 1200 Infinity Series Multisamplers User M anual 5 Optimizing Performance Delay Volume and Extra Column Volume 88 Delay Volume 88 How to Configure the Optimum Delay Volume 89 How to Achieve Higher Injection Volumes 94 How to Achieve High Throughput 97 How to Achieve Higher Resolution 98 How to Achieve Higher Sensitivity 101 How to Achieve Lowest Carry Over 102 This chapter gives hints on how to optimize the performance or use additional devices ee Agilent Technologies 87 5 Optimizing Performance Delay Volume and Extra Column Volume 88 The delay volume is defined as the system volume between the point of mixing in the pump and the top of the column The extra column volume is defined as the volume between the injection point and the detection point excluding the volume in the column Delay Volume In gradient separations this volume causes a delay between the mixture changing in the pump and that change reaching the column The delay depends on the flow rate and the delay volume of the system In effect this means that in every HPLC system there is an additional isocratic segment in the gradient profile at the start o
73. e the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet gt Ground the waste container gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly gt Do not use solvents with an auto ignition temperature below 200 C 392 F 1200 Infinity Series M ultisamplers User Manual 259 14 Appendix 260 Symbols Table 19 Symbols A i gt oe o e The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up Cooling unit is designed as vapor compression refrigeration
74. e9 Leak and waste handling with multisampler in a mixed configuration as an example 26 1200 Infinity Series M ultisamplers User Manual Introduction 1 System Overview Multisampler Base plate Figure 10 Leak and waste handling with multisampler in a mixed configuration as an example two stack configuration NOTE Do not place the multisampler directly on the bench If a sample cooler is installed install Base plate G1328 44121 underneath 1200 Infinity Series Multisamplers User M anual 27 1 28 Introduction System Overview 1200 Infinity Series M ultisamplers User Manual 1200 Infinity Series Multisamplers User M anual e a e r a o e W e 2 one Site Requirements and Specifications i J i Site Requirements 30 Physical Specifications 34 Performance Specifications 35 Performance Specifications G7167B 37 Performance Specifications G7167A 37 Physical Specifications of the Sample Cooler 40 This chapter provides information on environmental requirements physical and performance specifications ot Agilent Technologies 29 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 1 on page 34 Consequently there is no v
75. ead Assembly 40 pL Analytical Head Assembly 40 pL 206 ltem p n G4267 60042 G4267 60423 0905 1717 G4267 60422 0515 4384 G4267 60432 5067 5920 a A W N e l my Description Analytical Head 40 uL Head Assembly 40 uL Metering seal 40 uL Seal Support Assembly 40 uL Screw Spring Adapter Assembly Piston ceramic 4 eo Figure 35 Analytical head assembly 40 uL 1200 Infinity Series M ultisamplers User Manual Parts for Maintenance and Upgrade or Options 10 Analytical Head Assembly 100 pL Analytical Head Assembly 100 uL ltem p n G4267 60043 G4267 60433 0905 1719 G4267 60434 0515 1052 G4267 60432 5067 5678 a A W N e my 1 9 Description Analytical Head 100 uL Head Assembly 100 uL Metering seal 100 uL Seal Support Assembly 100 uL Screw 2 5mm hex Spring Adapter Assembly Piston 1290 Infinity Pumps ceramic 4 eo Figure 36 Analytical head assembly 100 uL 1200 Infinity Series M ultisamplers User Manual 207 10 Parts for Maintenance and Upgrade or Options Analytical Head Assembly 900 uL Analytical Head Assembly 900 uL ltem p n G4267 60046 G4267 60461 0905 1294 G4267 60463 0515 4864 G4267 60432 G4267 60462 a A W N e Description Analytical head 900 uL 400 bar Head Assembly 900 uL M etering seal 900 uL Seal Support Assembly 900 uL Screw for heat exchangers Spring Adapter Assembly Piston Assembly 900 uL Figure 37 Analy
76. eedle assembly is in maintenance position Maintenance Positions Task Selector Change Rator Seal No X Set gt gt Change Loop No Change Materrg Derce No Press the Stat gt gt bution in onder to begin Procease Change Seat No Grange the neede Leave Te service mode Change Netde Yes X Potom ato referencing Rees EMF Courters 2 If no needle assemby have to be changed click Next The Agilent Lab Advisor software will perform an auto referencing and reset the EMF counters 1200 Infinity Series M ultisamplers User Manual Test Functions and Calibration 8 Auto Referencing 3 Click the Back button to leave the Service amp Diagnosis menu 1200 Infinity Series M ultisamplers User Manual 123 8 Test Functions and Calibration Maintenance Positions When 124 Some maintenance procedures require the needle assembly the sample loop flex the metering device and the needle seat to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position In the Agilent Lab Advisor Software the maintenance positions can be selected in the Service amp Diagnostics view Performing maintenance on the module 1 Run the Maintenance Positions in the Service amp Diagnostics View in the Agilent Lab Advisor for further information see Online Help of user interface Ji Tests J Catros 7 Tods Diagnostic B ers HW x Road
77. een 1 12 and inert to many common solvents There is still a number of known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc Polyimide Agilent uses semi crystalline polyimide for rotor seals in valves and needle seats in autosamplers One supplier of polyimide is DuPont which brands polyimide as Vespel which is also used by Agilent Polyimide is stable in a pH range between 1 and 10 and in most organic solvents It is incompatible with concentrated mineral acids e g sulphuric acid glacial acetic acid DMSO and THF It is also degraded by nucleophilic substances like ammonia e g ammonium salts in basic conditions or acetates 1200 Infinity Series M ultisamplers User Manual 67 4 68 Preparing the module Polyethylene PE Agilent uses UHMW ultra high molecular weight PE PTFE blends for yellow piston and wash seals which are used in 1290 Infinity pumps and for normal phase applications in 1260 Infinity pumps Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12 5 It is compatible to many organic solvents used in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic
78. er Manual Optimizing Performance 5 ways N is inversely proportional to the particle size and directly proportional to the length of a column and so smaller particle size and a longer column will give a higher plate number The pressure rises with the inverse square of the particle size and proportionally with the length of the column This is the reason that the 1290 Infinity LC system was designed to go to 1200 bar so that it can run sub two micron particles and column length can be increased to 100 mm or 150 mm There are even examples of 100 mm and 150 mm columns linked to give 250 mm length Resolution increases with the square root of N so doubling the length of the column will increase resolution by a factor of 1 4 What is achievable depends on the viscosity of the mobile phase as this relates directly to the pressure Methanol mixtures will generate more back pressure than acetonitrile mixtures Acetonitrile is often preferred because peak shapes are better and narrower in addition to the lower viscosity but methanol generally yields better selectivity certainly for small molecules less than about 500 Da The viscosity can be reduced by increasing the temperature but it should be remembered that this can change the selectivity of the separation Experiment will show if this leads to increase or decrease in selectivity As flow and pressure are increased it should be remembered that frictional heating inside the column will increase and th
79. er peak height and is therefore ideal for applications with limited sample amounts If the same sample amount can be injected on a smaller i d column then the dilution due to column diameter will be less and the sensitivity will increase For example decreasing the column i d from 4 6 mm to 2 1 mm results in a theoretical gain in peak height of 4 7 times due to the decreased dilution in the column For a mass spectrometer detector the lower flow rates of narrow columns can result in higher ionization efficiencies and therefore higher sensitivity 1200 Infinity Series M ultisamplers User M anual 101 5 Optimizing Performance How to Achieve Lowest Carry Over 102 Carryover is measured when residual peaks from a previous active containing injection appear in a subsequent blank solvent injection There will be carry over between active injections which may lead to erroneous results The level of carryover is reported as the area of the peak in the blank solution expressed as a percentage of the area in the previous active injection The Multisampler is optimized for lowest carryover by careful design of the flow path and use of materials in which sample adsorption is minimized A carryover figure of 0 001 should be achievable even when a triple quadrupole mass spectrometer is the detector Operating settings of the Multisampler allow the user to set appropriate parameters to minimize carryover in any application involving compounds liab
80. ers into the sample vial or well plate 8 The metering device draws the preset sample volume 9 The needle lifts out of the sample vial or well plate 10 The coupled needle assembly robot is then moved to the park station onto the seat to close the sample loop 11 The needle assembly is locked into the park station and moves down 12 The injection cycle is completed when the injection valve switches to the mainpass position 1200 Infinity Series M ultisamplers User M anual Introduction 1 13 The robot moves the sample container back into the sample hotel if the sampling sequence is done If needle wash is required it will be done between step 9 and 10 For the needle seat backflush the M ultisampler must be in bypass mode If an additional needle seat backflush is required this step must also be done between step 9 and 10 Injection Sequence Before the start of the injection sequence and during an analysis the injection valve is in the mainpass position In this position the mobile phase flows through the Multisampler metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over oy Solvent Pump Sampling loop Wash i Metering device Multisampler Column Figure3 Valve in mainpass flow through When the sample sequence begins the valve unit switches to the bypass position Solvent from the pump enters the valve unit at port 1 and
81. etely filling of the peristaltic pump tubing before continuing to prime the peristaltic pump 8 Close the front door U 1200 Infinity Series M ultisamplers User M anual 171 9 Maintenance Replace the Flushhead Seal When Flush head is leaking Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2392 Hex key 4 mm15 cm long T handle 01018 23702 Insert tool OR G4226 43800 Seal insert tool Parts required p n Description 5067 5918 Seal 500 uL Preparations Cleaning tissue Appropriate solvent like isopropanol or methanol 1 Inthe Instant Pilot start the maintenance mo select Change metering device function OR In the Agilent Lab Advisor software select Service amp Diagnostics in the system screen Tools gt Maintenance Positions gt Change Metering click start and wait until the metering device maintenance position de and 2 Open the front door Device isin 172 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Replace the Flushhead Seal 4 Press and turn the Flush Head a quarter left bayonet fitting and detach the metering device from the actuator 6 Press against the rear side of flush head and turn a quarter left bayonet fitting and separate the flush head head body and the piston Be careful not to break the piston 1200 Infinity Series M ultisamplers User Manual 173 9 Maintenan
82. eve Higher Injection Volumes PumpA Autosampler Position 1 Position 2 Enrichment Pump B calumn Figure 21 Sample enrichment 96 1200 Infinity Series M ultisamplers User Manual Optimizing Performance 5 How to Achieve High Throughput The injection can be optimized for speed remembering that drawing the sample too fast can reduce the reproducibility Marginal gains are to be made here as the sample volumes used tend towards the smaller end of the range in any case A significant portion of the injection time is the time taken with the needle movements to and from the vial and into the flush port These manipulations can be performed while the previous separation is running This is known as overlapped injection and it can be easily turned on from the Multisampler setup screen in the control software The Multisampler can be told to switch the flow through the Multisampler to bypass after the injection has been made and then after for example 3 minutes into a 4 minutes run to start the process of aspirating the next sample and preparing for injection This can typically save 0 5 to 1 minute per injection 1200 Infinity Series M ultisamplers User Manual 97 5 Optimizing Performance How to Achieve Higher Resolution 98 Increased resolution in a separation will improve the qualitative and quantitative data analysis allow more peaks to be separated or offer further scope for speeding up the separation This section explain
83. f every run Usually the gradient profile is reported in terms of the mixture settings at the pump and the delay volume is not quoted even though this will have an effect on the chromatography This effect becomes more significant at low flow rates and small column volumes and can have a large impact on the transferability of gradient methods It is important therefore for fast gradient separations to have small delay volumes especially with narrow bore columns e g 2 1 mm i d as often used with mass spectrometric detection 1200 Infinity Series M ultisamplers User Manual Optimizing Performance 5 How to Configure the Optimum Delay Volume For very fast gradients over 0 5 min the delay volume of the system can be easily reduced without changing the physical configuration of the system The change is achieved by changing the behavior of the multisampler The 180 uL delay volume of the autosampler is due to the flow path from the injection valve through the metering device needle needle seat and connecting capillaries back to the injection valve see Table 10 on page 90 To make an injection the valve switches from mainpass to bypass so that the metering device can draw the sample into the needle capillary The injection is made when the valve switches back to mainpass and the sample is flushed onto the column The valve remains in this position during analysis so that the autosampler is continually flushed and hence the gradient has to fl
84. f the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface 1200 Infinity Series M ultisamplers User Manual Error Information 7 General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 A not ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required Shutdown Error ID 0063 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote i
85. fety related features are leak detection safe leak handling leak output signal for shutdown of pumping system and low voltages in major maintenance areas Early maintenance feedback EM F for continuous tracking of instrument usage with user settable limits and feedback messages Electronic records of maintenance and errors All materials recyclable Metering device in high pressure flow path 1 only for the integration in an Infinity LC setup 1200 Infinity Series M ultisamplers User Manual 39 2 Site Requirements and Specifications Physical Specifications of the Sample Cooler Cooling unit is designed as vapor compression refrigeration system Contains fluorinated greenhouse gas refrigerant according to the Kyoto protocol For specifications of refrigerant charge capacity carbon dioxide equivalent CDE and global warming potential GWP see instrument label Table 4 Physical Specification of the Sample Cooler Type Specification Comments Weight lt 6kg Dimensions height x width x 205 mm X 340 mm X 370 mm depth Refrigerant gas HFKW 134a 0 042 kg Ozone depletion potential ODP 0 Line voltage 24 VDC nominal Current 10 A max Ambient operating 4 40 C 39 2 131 F temperature Ambient non operating 40 70 C 20 158 F temperature Humidity 4 35 C 20 80 RH Non condensing Operating altitude Non operating altitude Safety standards IEC CSA UL Up to 3000 m 984
86. follow good laboratory practice 140 It is recommended to always exchange the needle assembly and the needle seat at the same time to prevent premature leakage 1200 Infinity Series M ultisamplers User Manual Maintenance 9 1 Inthe Instant Pilot start the maintenance mode and 2 Open the front door select Change needle seat function OR In the Agilent Lab Advisor software select Service amp Diagnostics in the system screen Tools Maintenance Positions gt Change Needle Loop click Start and wait until the needle assembly is in maintenance position Sharp needle Uncovered needles may cause injuries gt Do not unlock the saftey lock of needle assembly 1200 Infinity Series M ultisamplers User Manual 141 9 Maintenance Remove the Needle Assembly 3 Remove the needle assembly by slightly pulling the needle cartridge g 142 Z Robot Z arm coupler without the needle assembly CAUTION Damage of the loop The loop shape may be damaged if the loop is stretched or bent too far gt Avoid to change the loop shape gt Do not pull or bend the loop too far 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Remove the Needle Assembly 5 The needle assembly is still connected to the loop capillary Loop plastic adapter 6 Remove the loop plastic adapter 7 Useal 4inch wrench to loosen the fitting of the loop capillary
87. gned to bear the weight of all modules The module should be operated in a horizontal position especially if a sample cooler is installed Check position with a bulb Condensation CAUTION Condensation within the module Condensation can damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 32 1200 Infinity Series M ultisamplers User Manual Site Requirements and Specifications 2 Warranty Provisions and Reference Warranty provisions Agilent standard warranty does not cover Improper use or operation outside the product specification Defects due to adjustments or maintenance by customer or unauthorized parties Visible abuse negligence or shipping damage Unsupported configurations Improper site preparations Use of non Agilent parts Reference The removal of nameplates leads to loss of Warranty claim e Any unauthorized destruction of seals will invalidate the Warranty claim 1200 Infinity Series M ultisamplers User Manual 33 2 Site Requirements and Specifications Physical Specifications Table1 Physical Specifications Type Specification Comments Weight 22 kg 48 5 Ibs w o sample cooler Dimensions height x width 320 x 396 x 468 mm
88. he Module 14 The Multisampler transport mechanism uses a Cartesian robot The X Y drive together with the Z drive optimize the grabbing and positioning for the sample trays and the needle handling inside of the Multisampler The sample coupler moves the sample container from the sample hotel which stores all the samples and place it on the central workspace Then the needle coupler of the Z drive take over and grab the needle assembly from the needle station and perform the analytical procedures inside of the Multisampler Due the uncoupled needle design the robot can do other liquid handling jobs during the analysis The multisampler employ an active vial plate pusher mechanism to hold down the vial or the plate while the needle is drawn back from the sample vessel a must in the case a septum is used This active vial plate pusher employs a sensor to detect the presence of a plate and to ensure accurate movement regardless of plate used All axes of the transport mechanism are driven by very fast BLCD motors Optical encoders ensure the correct operation of the movement The standard metering device provides injection volumes from 0 1 20 uL A 0 1 40 uL injection volume metering device is installed in the G7167A B with a 20 uL low restriction loop capillary restricting the injection volume The entire flowpath including the metering device is always flushed by the mobile phase after injection for minimum internal carry over To redu
89. here with carryover If the problem compounds need a high percentage of organic phase for elution it is recommended to switch the injection valve at the high percentage of organic phase after the last peak has eluted It is also recommended to switch the injection valve again after the initial conditions for the mobile phase have stabilized This ensures that the bypass groove in the rotor seal of the valve contains the gradient start conditions which is especially important for flow rates below 0 5 mL min For samples where the outside of the needle cannot be cleaned sufficiently with water or alcohol from the flush pump use wash vials with an appropriate solvent With an injector program several wash vials can be used for cleaning 1200 Infinity Series M ultisamplers User Manual 103 5 Optimizing Performance The optimum carry over performance of the Multisampler is achieved after a run in period of new instruments or after the exchange of consumable parts like needle needle seat and valve parts During injections in this period surfaces of these parts adjust to each other After this period we recommend back flushing the needle seat in order to get the sealing areas between needle and needle seat clean Regular Preventive Maintenance service is recommended as the carry over performance of the Autosampler depends on the integrity of these consumable parts 104 1200 Infinity Series M ultisamplers User Manual 1200 Infinity Series Multisa
90. in your CDS system and your are not able to use the new drawers The new drawer configuration is active and stored after you have done the Drawer Configuration 192 1200 Infinity Series M ultisamplers User Manual Maintenance 9 tha Nimmy N 1 e the Dummy Draw Configure the Hotel Drawers in the Control Software Software required OpenLAB A 02 01 or above LC driver A 02 10 or above Preparations Stop the acquisition run Remove the sample containers trays and well plates from workspace Complete the drawer installation Remove the sample containers trays and well plates from the drawers Verify that all sample trays palettes are installed in their drawers All open drawers and dummies have to be closed and installed properly 1 Start OpenLAB CDS ChemStation Edition 2 Right click on the Multisampler GUI Multisampler amp O0 2g Idie M 3 Select Modify gt Drawer Configuration in the GUI screen TE For correct detection it is necessary to remove all sample containers for example 54 vial tray or well plates 1200 Infinity Series M ultisamplers User Manual 193 9 Maintenance Replace the Dummy Drawer 4 Follow the Setup or Change configuration screen 5 System is ready after the robot has done Auto Referencing 194 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Configure the Hotel Drawers in Lab Advisor Software required Lab Advisor B 02 05 or above Preparati
91. isamplers User Manual 181 9 Maintenance Removing the Sample Loop Flex 5 Remove the cartridge out of its proper position By gently 6 Remove the loop plastic adapter tilting and pulling it out of the work space of the multisampler 182 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Removing the Sample Loop Flex 7 Usea1 4inch wrench to loosen the fitting of the loop 8 Remove the needle assembly capillary 1200 Infinity Series M ultisamplers User Manual 183 9 Maintenance Installing the Sample Loop Flex When If the sample loop flex is defective or damaged Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch Parts required p n Description 64267 60300 Sample Loop Flex 20 uL right red coded G4267 60400 Sample Loop Flex 40 uL right green coded G4267 60500 Sample Loop Flex 100 uL right blue coded Preparations Finish any pending acquisition job and return any plate on the workspace back to the hotel WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Do not open the safety lock of the needle assembly gt Be careful working at the z robot gt Wear safety goggles when removing the needle assembly 184 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Installing the Sample Loop Flex 1 Install the loop capillary on top of the needle cartridge 1 and tighten
92. ity to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like run synchronization through APG remote error handling diagnostic functions or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance Firmware Updates Firmware updates can be done using the following tools latest version should be used 1200 Infinity Series M ultisamplers User Manual Hardware Information 12 Agilent Lab Advisor software with files on the hard disk Firmware Update Tool with local files on the hard disk Instant Pilot G4208A with files on a USB Flash Disk Required tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315B for the G1315B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 650 is revision 6 50 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Upd
93. l the parts with an appropriate solvent taking care that no surfaces get scratched CAUTION Damage to the rotor seal and cross port leaks gt Before you replace the rotor seal clean the stator gt Inspect the stator head and swab it with the appropriate solvent If more stringent cleaning is required use a sonicator Inspect the remaining valve components for contamination Clean them as necessary gt If the stator head is scratched replace the valve 6 Install new rotor seal NOTE M ake sure that the rotor sealing surface with its engraved flow passages is facing out The pattern is asymmetrical to prevent improper placement 154 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Replace the Rotor Seal 7 Reinstall the stator head The index pins on the drive and the stator head must engage in the corresponding holes Insert the two socket head screws SS 8 Using a 9 64 in L Hex wrench tighten each screw gently until you feel resistance approximately fingertight Tighten each screw by 1 8 turn and then tighten each screw again until the stator is secured to the driver NOTE Do not over tighten the screws The screws hold the assembly together and do not affect the sealing force The sealing force is automatically set as the screws close the stator head against the valve body 1200 Infinity Series M ultisamplers User Manual 155 9 Maintenance
94. le Assembly 3 Install loop plastic adapter 4 Pinch and reinsert the needle assembly and the connected loop capillary into the z arm coupler NOTE Check the tension of the loop capillary This must be forced and guided to the hydraulic box to prevent it from being caught by the Z drive 146 1200 Infinity Series M ultisamplers User Manual Maintenance 9 5 Inthe Instant Pilot close Change needle seat 6 Close the front door OR In the Agilent Lab Advisor software Change needle loop gt End click End and wait until the needle assembly is in the needle park station A Eal 7 Perform a pressure test 1200 Infinity Series M ultisamplers User Manual 147 9 Maintenance Exchange the Needle Seat When When seat is visibly damaged blocked or leaks Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch Flat head screwdriver Parts required p n Description G4267 87012 High Pressure Needle Seat 0 12 mm PEEK Preparations In order to avoid leaks stop the pump running and remove the tubings from the solvent bottles If available close the shutoff valves WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Do not open the safety lock of the needle assembly gt Be careful working at the z robot gt Wear safety goggles when removing the needle assembly 1 Inthe Instant Pilot
95. le instrument You can for example optimize one path for large volume injections and the other for low delay volumes Ultralow carryover 1290 Infinity II Multisampler is designed for low carryover but you can take clean to a whole new level with our multi wash capability cleansing all relevant injection parts between runs This sophisticated integrated feature flushes the injection needle outside with three solvents and uses seat backflush procedures to reduce carryover to less than 10 ppm Efficient temperature control For temperature sensitive samples simply add Agilent s new highly efficient compressor based cooling system It allows you to maintain perfect temperature control on all vials and plates inserted into the 1290 Infinity Multisampler Instant information Lights on each drawer tell you all you need to know about loading status current activity and accessibility 1200 Infinity Series M ultisamplers User Manual Introduction 1 Features G7167A Unmatched flexibility You can choose how you want to introduce samples for injection whether you prefer vials microtiter plates or any combination of formats Sample drawers are available in three heights and you can mix shallow drawers with deeper ones to accommodate different sample sizes High capacity Using shallow well plate drawers the 1260 Infinity Multisampler takes a maximum load of 16 microtiter plates and up to 6144 samples the most of
96. le to stick in the system The following functions of the Multisampler can be used to minimize carryover Internal needle wash External needle wash Needle seat backflush Injection valve cleaning The flow path including the inside of the needle is continuously flushed in normal operation providing good elimination of carryover for most situations Automated delay volume reduction ADVR will reduce the delay volume but will also reduce the flushing of the Standard Multisampler and should not be used with analytes where carryover might be a problem The outside of the needle can be washed using a wash vial in a specific location or the needle can be washed using the flush port If a wash vial in a tray location specified by the user is chosen then this vial should have no septum and should contain a solvent suitable for washing the sample from the needle The septum is not used to avoid wiping contamination off the needle on the downstream only to re apply it on the upstroke The needle can be dipped into the vial multiple times This will be effective in removing a small degree of carryover but for more effective washing of the outside of the needle use the flushport 1200 Infinity Series M ultisamplers User Manual Optimizing Performance 5 The flush port is located above and behind the needle seat and in the standard hardware configurationa peristaltic pump delivers the wash solvent It has a volume of 0 68 mL and the peris
97. lent Technologies to ensure proper functionality and compliance with safety or EM C regulations Analog cables p n 35900 60750 01046 60105 Remote cables p n 5188 8029 5188 8044 5188 8045 5061 3378 01046 60201 CAN cables p n 5181 1516 5181 1519 220 Description Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description ERI to general purpose Remote Cable ERI ERI Remote Cable APG ERI Remote Cable to 35900 A D converter Agilent module to general purpose Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m 1200 Infinity Series M ultisamplers User Manual LAN cables p n 5023 0203 5023 0202 RS 232 cables notfor FUSION p n board 1530 60600 RS232 61601 5181 1561 USB cables p n 5188 8050 5188 8049 Description Identifying Cables 11 Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Description RS 232 cab RS 232 cab e 2m e 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Nul Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cab e 8m Description USB AM U SB Mini B 3 m PC M odule
98. llary Color Coding Guide 73 Installing Capillaries 74 1200 Infinity Series M ultisamplers User Manual Contents Setting up the Autosampler with Agilent Open Lab ChemStation 77 Optimizing Performance 87 Delay Volume and Extra Column Volume 88 How to Configure the Optimum Delay Volume 89 How to Achieve Higher Injection Volumes 94 How to Achieve High Throughput 97 How to Achieve Higher Resolution 98 How to Achieve Higher Sensitivity 101 How to Achieve Lowest Carry Over 102 Troubleshooting and Diagnostics 105 User Interfaces 106 Agilent Lab Advisor Software 107 Error Information 109 What Are Error Messages 110 General Error Messages 111 Test Functions and Calibration 117 Introduction 118 System Pressure Test 119 Auto Referencing 122 Maintenance Positions 124 Injector Steps 127 Maintenance 129 Introduction to Maintenance 131 Warnings and Cautions 133 Overview of Maintenance 135 Clean the Module 136 Removal and Installation of the Front Door 137 Remove the Needle Assembly 140 Install the Needle Assembly 144 Exchange the Needle Seat 148 Replace the Rotor Seal 151 1200 Infinity Series M ultisamplers User M anual Remove the Metering Seal 157 Install the Metering Seal 162 Replace the Peristaltic Pump Cartridge 168 Replace the Flushhead Seal 172 Replace the Injection Valve 176 Removing the Sample Loop Flex 180 Installing the Sample Loop Flex 184 Replace the Dummy Drawer 187 Remove the Sample Cooler 196
99. long Thread groove too short fot Vici valves Figure 12 Capillary connections for the multisampler Note that the SL fittings are backward compatible to the IDEX valves Parts required p n Description 5067 4650 Capillary ST 0 12 mm x 150 mm SL SX 5067 4651 Capillary ST 0 12 mm x 280 mm SL SX 5067 4720 Capillary ST 0 17 mm x 150 mm SL SX 5067 4722 Capillary ST 0 17 mm x 280 mm SL SX 5065 4454 Fitting screw long 10 pk Quantity depends on configuration of the module number of connections to the multisampler 74 1200 Infinity Series M ultisamplers User M anual Preparing the module The capillaries mentioned above are examples only 1 Select a nut that is long enough for the fitting you ll be using So Gp 2 Slide the nut over the end of the tubing 3 Carefully slide the ferrule components on after the nut and then finger tighten the assembly while ensuring that the tubing in completely seated in the bottom of the end fitting _ Ome 4 Usea column or injection valve to gently tighten the fitting which forces the ferrule to seat onto the tubing Don t overtighten Overtightening will shorten the lifetime of the fitting 1200 Infinity Series Multisamplers User Manual 4 Preparing the module Example of a perfect fitting 5 Once you believe you have the fitting complete loosen the nut and inspect the ferrule for the correct position on the tubing Example of a perfect fitting Ex
100. ltisampler or via right click on the Active area NOTE The signal window in the lower part is not shown when opening the parameter settings via right mouse on the M ultisampler user interface 82 1200 Infinity Series M ultisamplers User Manual Preparing the module 4 Setting up the Autosampler with Agilent Open Lab ChemStation Figure 15 Method parameter settings NOTE For additional help and support Highlight the desired cell and press the F1 key A help screen will open with additional information and documentation about the topic 1200 Infinity Series Multisamplers User M anual 83 4 Preparing the module 5 Pema Lome so Injection Mode Needle Wash The settable Injection volume is depending on what kind of configuration is installed Default configuration 0 1 20 uL It is possible to select between using the Standard Wash or Standard Wash off Using needle wash is one option to obtain minimum carry over The Injection cleaning section allows you to select between the Standard Wash option and the Multi wash option With the Standard Wash default configuration you can choose between two modes the Flush port or Wash Vial If the Multi Wash option is installed additional hardware is required you can use Needle Wash and Seat Back Flush together to obtain the lowest carry over Multi wash M ultisampler Injection Cleaning The M ulti wash table allows you to specify up to four steps that will be used to clean the
101. lvent then the volume can probably be increased further because the effect will be to concentrate the analyte on the head of the column at the 1200 Infinity Series M ultisamplers User Manual Optimizing Performance 5 start of the gradient Conversely if the injection is in a stronger solvent than the starting mobile phase then increased injection volume will spread the band of analyte down the column ahead of the gradient resulting in peak dispersion and loss of resolution Perhaps the main consideration in determining injection volume is the diameter of the column as this will have a big impact on peak dispersion Peak heights can be higher on a narrow column than with a larger injection on a wider column because there is less peak dispersion With 2 1 mm i d columns typical injection volumes might range up to 5 to 10 uL but it is very dependent on the chemistry of the analyte and mobile phase as discussed above In a gradient separation injection volumes of about 5 of the column volume might be achieved whilst maintaining good resolution and peak dispersion One way to achieve larger injections is to use a trapping column selected by a switching valve to capture and concentrate the injection before switching it i e injecting it onto an analytical column see Figure 21 on page 96 The valve can be conveniently located in the Multicolumn Thermostat 1200 Infinity Series M ultisamplers User Manual 95 5 Optimizing Performance How to Achi
102. moval and Installation of the Front Door 3 For the Installation of the front door Insert the hinges into their guides and move the door in until the release buttons click into their final position 1200 Infinity Series M ultisamplers User Manual 139 9 Maintenance Remove the Needle Assembly When Tools required Parts required OR Preparations When the limit in the needle into seat counter in the EM F is exceeded or when needle shows indications of damage blockage or leaks p n Description 8710 0510 Wrench open 1 4 5 16 inch p n Description 1 64267 87201 Needle Assembly 1 G4267 87210 Needle Assembly slotted for high injection volumes In order to avoid leaks stop the pump running and remove the tubings from the solvent bottles If available close the shutoff valves Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Do not open the safety lock of the needle assembly gt Be careful working at the z robot gt Wear safety goggles when removing the needle assembly Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and
103. mp e e 00 ee 7 209 Error Information e s j What Are Error Messages 110 4 General Error Messages 111 Timeout 111 Shutdown 111 Remote Timeout 112 Lost CAN Partner 113 Leak Sensor Short 113 LeakSensorOpen 114 Compensation SensorOpen 114 Compensation SensorShort 115 Fan Failed 115 Leak 116 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions ot Agilent Technologies 109 7 Error Information W hat Are Error Messages 110 Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started i
104. mplers User M anual r a 0o90 ee 6 one Troubleshooting and Diagnostics User Interfaces 106 Agilent Lab Advisor Software 107 This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces gg Agilent Technologies 105 6 Troubleshooting and Diagnostics User Interfaces Depending on the user interface the available tests and the screens reports may vary Preferred tool should be Agilent Lab Advisor Software see Agilent Lab Advisor Software on page 107 The Agilent OpenLab ChemStation C 01 03 and above do not include any maintenance test functions Screenshots used within these procedures are based on the Agilent Lab Advisor Software 106 1200 Infinity Series M ultisamplers User Manual Troubleshooting and Diagnostics 6 Agilent Lab Advisor Software The Agilent Lab Advisor Software is a standalone product that can be used with or without chromatographic data system Agilent Lab Advisor helps to manage the lab for high quality chromatographic results by providing a detailed system overview of all connected analytical instruments with instrument status Early Maintenance Feedback counters EMF instrument configuration information and diagnostic tests By the push of a button a detailed diagnostic report can be generated Upon request the user can send this report to Agilent for a significantly improved troubleshooting and repair process
105. mplers User Manual Maintenance 9 Install the Sample Cooler Power switch 1 On 2 Off 1200 Infinity Series M ultisamplers User Manual 199 9 Maintenance Replace the M odule Firmware When Tools required OR Parts required Preparations 200 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary e to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or e if third party control software requires a special version Description Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN USB FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx w hid 69761 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module 1200 Infinity Series M ultisamplers User Manual 1200 Infinity Series Multis
106. mproper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module WARNING Sharp metal edges Sharp edged parts of the equipment may cause injuries gt To prevent personal injury be careful when getting in contact with sharp metal areas 1200 Infinity Series M ultisamplers User Manual 133 9 Maintenance WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere CAUTION S
107. nditions again 1200 Infinity Series M ultisamplers User Manual Introduction 1 Multisampler Principle veel device Multisampler Column Figure7 Valve in bypass needle backflush M ultiwash 1200 Infinity Series M ultisamplers User Manual 21 1 Introduction System Overview Leak and Waste Handling The 1290 Infinity II Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed 22 1200 Infinity Series M ultisamplers User Manual Introduction 1 System Overview Figure8 Leak and waste handling concept overview typical stack configuration as an example The solvent cabinet is designed to store a maximum volume of 6 L solvent The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 4 L For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets a printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet 1200 Infinity Series M ultisamplers User Manual 23 1 24 Introduction All leak plane outlets are situated in a consistent position so that all Infinity and Infinity II modules can be stacked on top of each other Waste tubes are guided through a channel on the right hand side of the instrument keeping the front access clear from tubes The leak plane provides leak management b
108. nerated Probable cause Suggested actions 114 1 Loose connection between the power switch board and the main board 2 Defective power switch board Please contact your Agilent service representative Please contact your Agilent service representative 1200 Infinity Series M ultisamplers User Manual Error Information 7 Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective power switch board Please contact your Agilent service representative 2 Loose connection between the power Please contact your Agilent service switch board and the main board representative Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable
109. nert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications 1200 Infinity Series M ultisamplers User M anual 69 4 70 Preparing the module Fluorinated polymers PTFE PFA FEP FFKM Fluorinated polymers like PTFE polytetrafluorethylene PFA perfluoroalkoxy and FEP fluorinated ethylene propylene are inert to almost all common acids bases and solvents FFKM is perfluorinated rubber which is also resistant to most chemicals As an elastomer it may swell in some organic solvents like halogenated hydrocarbons TFE PDD copolymer tubings which are used in all Agilent degassers except 1322A are not compatible with fluorinated solvents like Freon Fluorinert or Vertrel They have limited life time in the presence of Hexafluoroisopropanol HFIP To ensure the longest possible life with HFIP it is best to dedicate a particular chamber to this solvent not to switch solvents and not to let dry out the chamber For optimizing the life of the pressure sensor do not leave HFIP in the chamber when the unit is off Sapphire Ruby and Al 03 based ceramics Sapphire ruby and ceramics based on aluminum oxide Al Og are inert to almost all common acids bases and solvents There are no documented incompatibilities for HP
110. nformation on the instrument labels provide the following information CCYWWSSSSS Format cc country of manufacturing DE Germany JP apan CN China 236 1200 Infinity Series M ultisamplers User M anual YWW SSSSS Hardware Information 12 year and week of last major manufacturing change e g 820 could be week 20 of 1998 or 2008 real serial number Serial Number Information 1260 Infinity The serial number information on the instrument labels provide the following information CCXZZ00000 CC ZZ 00000 1200 Infinity Series M ultisamplers User Manual Format Country of manufacturing DE Germany e JP Japan CN China Alphabetic character A Z used by manufacturing Alpha numeric code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same module Serial number 237 12 Hardware Information Interfaces The Agilent 1200 Infinity Series II modules provide the following interfaces Table 15 Agilent 1200 Infinity II Series Interfaces Module CAN USB LAN RS 232 Analog APG Special on board A ERI E Pumps G7104A Flexible Pump 2 No Yes Yes 1 A G7120A High Speed Pump 2 No Yes Yes 1 A Samplers G7129A B ALS 2 Yes Yes No No E G7167A B Multisampler 2 Yes Yes No No E Detectors G7114A B VWD 2 Yes Yes No 1 E G7117A B DAD 2 Yes Yes No 1 E G7115A B DAD 2 Yes Yes No 1 E Others G7116B MCT 2 No No No No No Requires a HOST mod
111. ng Cables 11 p n 01046 60201 Wire Color Pin Agilent SignalName Active module TTL White 1 Digital ground A o 1 oo Brown 2 Prepare run Low 00 p KEY Gray 3 Start Low oo o0 S H Blue 4 Shut down Low am Pink 5 Not oo connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 1200 Infinity Series M ultisamplers User M anual 227 11 Identifying Cables CAN LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection 228 1200 Infinity Series M ultisamplers User Manual Agilent M odule to PC p n G1530 60600 RS232 61601 5181 1561 Description Identifying Cables 11 RS 232 cable 2m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not co called Nul mpatible with connecting printers and plotters It s also M odem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8m 1200 Infinity Series M ultisam
112. niature 15 pin 224 1200 Infinity Series M ultisamplers User Manual Table 14 5188 8044 ERI to ERI Identifying Cables 11 p n 5188 8044 Pin ERI Signal Pin ERI 10 GND 10 ma 3 1 Start Request 1 2 Stop 2 3 Ready 3 5 Power on 5 4 Future 4 6 Shut Down 6 7 Start 7 8 Prepare 8 Ground Cable Shielding NC Connection 5188 8045 ERI to APG Connector D_Subminiature 15 pin ERD Connector D_Subminiature 9 pin APG p n 5188 8045 Pin ERI Signal Pin APG 10 GND 1 7 1 Start Request 9 2 Stop 8 3 Ready 7 5 Poweron 6 4 Future 5 6 Shut Down 4 7 Start 3 8 Prepare 2 Ground Cable Shielding NC Connection 225 1200 Infinity Series M ultisamplers User M anual 11 Identifying Cables One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent M odule to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent SignalName Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 226 1200 Infinity Series M ultisamplers User Manual Agilent M odule to General Purpose Identifyi
113. nput connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message 1200 Infinity Series M ultisamplers User Manual 111 Error Information Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing Remote Timeout Error ID 0070 Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition Check the vacuum degasser for an error condition Refer to the Service M anual for the degasser or the 1260 pump that has the degasser built in A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the inst
114. nstrument Cover WARNING Instrument covers removed Electrical shock gt Do Not Remove the Instrument Cover gt Only Agilent authorized personnel are allowed to remove instrument covers Always disconnect the power cables and any external circuits before removing the instrument cover Do Not Modify the Instrument Do not install substitute parts or perform any unauthorized modification to the product Return the product to an Agilent Sales and Service Office for service and repair to ensure that safety features are maintained In Case of Damage WARNING Damage to the module Personal injury for example electrical shock intoxication gt Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel 258 1200 Infinity Series M ultisamplers User Manual Appendix 14 Solvents WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operat
115. nual For connecting the Agilent 1290 Infinity equipment to non Agilent 1290 Infinity equipment see Cable Overview on page 220 16 Connect the power cable to the power connector at the rear of the module 17 Power switch 1 On 2 Off 1200 Infinity Series M ultisamplers User M anual 57 3 Using the Module Transporting the M ultisampler with a Sample Cooler Installed There are magnets in the front area of the multisampler see M agnets on page 44 When moving the multisampler around the laboratory make sure that any condensed water inside the thermostat is removed e Tilt the module to the back so that the water inside the thermostat can safely flow into the leak funnel Otherwise no special precautions are needed for the modules Heavy weight The module is heavy gt 22 kg gt 46 lbs gt Carry the module at least with 2 people gt Avoid back strain or injury by following all precautions for lifting heavy objects gt Ensure that the load is as close to your body as possible gt Ensure that you can cope with the weight of your load CAUTION Mechanical damage of the module If the transport assembly is not parked and not protected by the transport foam the module could be damaged due to excessive shock of the shipping container during transport gt Always park the transport assembly before shipment gt Store the installation foam in a save place to u
116. ocket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1200 Infinity Series M ultisamplers User Manual 31 2 Site Requirements and Specifications Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Bench Space The module dimensions and weight see Table 1 on page 34 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 3 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is desi
117. oltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only WARNING The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed 30 1200 Infinity Series M ultisamplers User Manual CAUTION Site Requirements and Specifications 2 Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall s
118. omponents to predefined positions enabling the specific step to be done 1 Run the Injector Steps in the Service amp Diagnostics View in the Agilent Lab Advisor for further information see Online Help of user interface J Tess Ji Caltvations J Toots G 16 7A 12760 MLS Serial 3 DEA 1200 Infinity Series M ultisamplers User M anual 127 128 Test Functions and Calibration Injector Steps 2 Select the individual step command like needle selection and needle position for further information see Online Help of user interface Injector Steps Tray Selection Needle Sainction Device Status Net Racy Frort Neede fef r Neede 2 Hit Oem Neede Postion Row 1 Coum 1 E Neede Up ize i z Netde To Home Drow Parameters Volume 1 u Crew Speed Maimun us Purge hone Vave Bos Manoass 1200 Infinity Series M ultisamplers User M anual 1200 Infinity Series Multisamplers User Manual 9 Maintenance Introduction to Maintenance 131 Warnings and Cautions 133 Overview of Maintenance 135 CleantheModule 136 Removal and Installation of the Front Door 137 Remove the Needle Assembly 140 Install the Needle Assembly 144 Exchange the Needle Seat 148 Replace the Rotor Seal 151 Remove the M etering Seal 157 Install the M etering Seal 162 Replace the Peristaltic Pump Cartridge 168 Replace the Flushhead Seal 172 Replace the Injection Valve 176 Removing the Sample Loop Flex 180 Installing the Sample Loop Flex
119. ondition and properly tightened Perform the Pump Head Leak test Tighten the fittings or replace capillaries Perform the Autosampler Leak test Replace the TCC valve rotor seal Notice the difference between error in the test and a failed result An error is caused by an abnormal termination during the operation of the test whereas a failed result indicates that the test result were not within the specified limits 1200 Infinity Series M ultisamplers User Manual 121 8 Test Functions and Calibration Auto Referencing When Preparations 122 The multisampler auto referencing uses predefined positions on the base plate and the sample hotel to calibrate the positioning of the needle parkstation and the sample hotel The auto referencing is required to compensate deviations in positioning the needle assembly and the sample tray The auto referencing is required after disassembling the system or when you exchange the sample handler the sample hotel the needle parkstation the needle assembly or one of the main boards This function is implemented in the drawer detection and in the needle exchange routine After disassembling the module or an exchange of the needle assembly e Workspace of the multisampler is empty All drawers are closed properly 1 In the Agilent Lab Advisor software select Service amp Diagnostics in the system screen Maintenance Positions gt Change Needle select YES click Start and wait until the n
120. ons Stop the acquisition run Complete the drawer installation Remove the sample containers trays and well plates from workspace Remove the sample containers trays and well plates from the drawers Verify that all sample trays palettes are installed in their drawers All open drawers and dummies have to be closed and installed properly 1 Start the Lab Advisor Software 2 Connect the instrument and select Instrument Control in the system screen 3 Switch In the Configuration menu of the Multisampler Select Detect Drawers in the Hotel Configuration Configuration Set Plate Type Drawers Piste type Front gt gt gt none lt lt lt few 3b4RetSpecial 1SvialPiane JAAgilect 34PCR_EPOF S vielPine Sigilo 4 Matari lor tapa bor Symge 40 4 4267 50042 X Seat Capillary Seat assembly 0 12 mm 1250F Loop Capilary Flex 20d Lett 4267 50301 Hotel Corfiguraton Detect Orawers Ato Relerenang 4 Follow the Detect Hotel Configuration screen to detect the physically available drawers For correct detection it is necessary to remove all sample containers for example 54 vial tray or well plates 5 System is ready after the robot has done Auto Referencing 1200 Infinity Series M ultisamplers User Manual 195 9 Maintenance Remove the Sample Cooler The module is heavy gt 22 kg gt 46 lbs gt Carry the module at least with 2 people gt Avoid back strain
121. oop flex 180 repairs replacing firmware 200 resolution Optimization 98 rotor seal replace 151 RS 232C cable 229 S safety class 256 safety general information 256 standards 34 symbols 260 sample cooler 218 installation 51 install 197 remove 196 sample loop flex install 184 1200 Infinity Series M ultisamplers User Manual removal 180 weight 34 sampler main assemblies 203 tubing kit 217 sensitivity optimization 101 serial number information 236 237 setup LAN 252 shutdown 111 site requirements powercords 31 solvents 267 sound emission 266 special interfaces 242 specification physical 34 System pressure test errormessage 121 T temperature sensor 116 timeout 111 transport 58 troubleshooting error messages 110 tubing kit sampler 217 V vials 71 voltage range 34 W waste electrical and electronic equipment 262 WEEE directive 262 1200 Infinity Series M ultisamplers User Manual Index 271 www agilent com In This Book This manual contains technical reference information about the Agilent 1290 Infinity II Multisampler G7167B and the Agilent 1260 Infinity Multisampler G7167A The manual describes the following Introduction Site requirements and specifications Using the module Preparing the module Optimizing performance Troubleshooting and diagnostics Error information Test functions Maintenance Parts Hardware info
122. or SD lt 10 nL whatever is greater Up to 600 bar G7167A 0 2 5cp analytical head Pressure range up to 600 bar M ulti draw modus Injection into needle seat capillary Up to 600 bar using 40 uL analytical head Up to 600 bar using 100 uL analytical head M ulti load M easured caffeine M ax pressure for basic instrument 1200 Infinity Series M ultisamplers User Manual 37 2 Site Requirements and Specifications Table 3 Type Specification Agilent 1260 Infinity M ultisampler G7167A Performance Specifications Comment Sample capacity Injection cycle time Carry Over Multiwash Control and data 1H Drawer up to 8 drawers and 16 positions Shallow well plates MTP 2H Drawer up to 4 drawers and 8 positions MTP deep well plates vials Eppendorf 3H Drawer up to 2 drawers and 4 positions MTP deep well plates vials up to 6 mL Eppendorf lt 10 s using following standard conditions Default draw speed 100 uL min Default eject speed 400 uL min Injection volume luL lt 5s using a Dual needle setup lt 0 003 30 ppm M ultisampler Standard lt 0 0009 9 ppm M ultisampler M ultiwash Outer needle wash and seat backflush for carryover reduction with up to 3 different solvents Agilent Open Lab CDS M ax 6144 1536 samples 384M TP 96 3072 samples 432 vials 2 mL 1536 samples 60 vials 6 mL 384 vials 1 mL 216 vials 2 mL Using standard Single needle setup Tim
123. oth times After an installation Ethanol or methanol Alternative to isopropanol second choice if no isopropanol is available 1200 Infinity Series M ultisamplers User Manual Preparing the module 4 Table6 Choice of Priming Solvents for Different Purposes Activity Solvent To clean the system when Bidistilled water using buffers Comments Best solvent to re dissolve buffer crystals After a solvent change Bidistilled water Best solvent to re dissolve buffer crystals 1200 Infinity Series M ultisamplers User Manual 65 4 Preparing the module Solvent Information 66 Observe the following recommendations on the use of solvents Follow recommendations for avoiding the growth of algae see pump manuals Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Recommended Wash Solvents water ethanol methanol water acid especially for basic compounds water base especially for acidic compounds water acetonitrile Material Information Materials in the flow path are carefully selected based on Agilent s experiences in developing highest quality instruments for HPLC analysis over several decades Thes
124. ow through this delay volume to reach the column This can be eliminated by switching the injection valve from mainpass to bypass after the injection has been made and the injected sample has been flushed onto the column In practice this can be done a few seconds after injection and is activated by selecting the Automatic Delay Volume Reduction ADVR function in the autosampler setup menu The Flush out Factor typically 5 times injection volume ensures that enough time is allowed to flush the sample out of the injector before switching to bypass For instance a 1 uL injection under standard conditions effectively reduces the system delay volume by approximatly 160 uL 1200 Infinity Series M ultisamplers User Manual 89 5 Optimizing Performance Table 10 Schematic of injection steps in 1290 Infinity II M ultisampler oy Solvent Pump Wash Sampling loop a Metering device Multisampler Column Figure 16 Valve in mainpass flow through oy Solvent Pump Sampling loop Wash Wash Needle Waste port seat O g Column z Metering device Multisampler Figure 17 Valve in bypass drawing sample 90 1200 Infinity Series M ultisamplers User Manual Optimizing Performance 5 How to Configure the Ontimum Delay rmo COMNQUIS MC UPUMUM Delay Volume Table 10 Schematic of injection steps in 1290 Infinity II M ultisampler Solvent Pump Sampling loop Wash Needle Waste
125. paring the module 4 e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Diamond Like Carbon DLC Diamond Like Carbon is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fused silica and Quartz Si0 Fused silica is used in 1290 Infinity Flow Cells and capillaries Quartz is used for classical flow cell windows It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended Gold Gold is inert to all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia Zirconium Oxide Zr0 gt Zirconium Oxide is i
126. plers User Manual 229 11 Identifying Cables USB To connect a USB Flash Drive use a USB OTG cable with Mini B plug and A socket p n Description 5188 8050 USB A M USB Mini B 3 m PC M odule 5188 8049 USB A F USB Mini B M OTG Module to Flash Drive 1200 Infinity Series M ultisamplers User Manual 1200 Infinity Series Multisamplers User M anual 12 Hardware Information Firmware Description 232 Electrical Connections 235 Rear view ofthe module 236 Information on Instrument SerialNumber 236 Interfaces 238 Overview Interfaces 239 ERI Enhanced Remote Interface 243 Setting the 6 bit Configuration Switch 246 Instrument Layout 248 Early Maintenance Feedback 249 This chapter describes the module in more detail on hardware and electronics ot Agilent Technologies 231 12 Hardware Information Firmware Description 232 The firmware of the instrument consists of two independent sections e a non instrument specific section called resident system an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are e the complete communication capabilities CAN LAN and RS 232C memory management ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN and RS 232C memory management abil
127. r Manual Index A accessory kit standard 216 Agilent Lab Advisor software 107 Agilent Lab Advisor 107 Agilent oninternet 268 algae 267 ambient non operating temperature 34 ambient operating temperature 34 analog signal 241 analytical head assembly 100 207 40 206 apgremote 241 assemblies main 203 assembly door 215 needle port 214 B bench space 32 C cable CAN 228 LAN 228 overview 220 RS 232 229 cables analog 222 remote 224 CAN cable 228 cleaning 136 coding color 73 guide 73 color coding 73 guide 73 compensation sensor open 114 compensation sensor short 115 condensation 32 configuration module 85 options 187 control settings 81 D defect on arrival 50 delay volume description 88 dimensions 34 door assembly 215 doors installation 137 removal 137 drawer upgrade installation 189 drawer status indicator 47 electrical connections descriptions of 235 1200 Infinity Series M ultisamplers User Manual Index electronic waste 262 EMF early maintenance feedback 249 error messages compensation sensor open 114 compensation sensor short 115 fan failed 115 leak sensor open 114 leak sensor short 113 leak 116 lost CAN partner 113 remote timeout 112 shutdown 111 system pressure test 121 timeout 111 extra column volume 88 F fan failed 115 firmware description 232 main system 232 resident system 232 update tool 233 updates 232 200 upgrade downgrade 200 flush he
128. raulic box Rotate until the unions at the base of the replacement injection valve head and the valve actuator engage OR If the outside pin does not fit into the outside groove you have to turn the valve head until you feel that the two pins Snap into the grooves Now you should feel additional resistance from the valve drive while continue turning the valve head until the pin fits into the groove 180 NOTE Check the orientation of the rear side Verify the correct position of the Valve TAG 1200 Infinity Series M ultisamplers User M anual 177 9 Maintenance Replace the Injection Valve 5 Continue to rotate until the clocking pin in the injection 6 Replace the spanner nut 1 and tighten clockwise 2 valve head align with the notch in the housing and press Hand tighten only do not use wrenches on the spanner the replacement injection valve head into the actuator nut 178 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Replace the Injection Valve 7 Reconnect the capillaries 1200 Infinity Series M ultisamplers User Manual 179 9 Maintenance Removing the Sample Loop Flex When If the sample loop flex is defective or damaged Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch Parts required p n Description G4267 60300 Sample Loop Flex 20 uL right red coded 64267 60400 64267 60500 Sample Loop Flex 40 uL right green code
129. rawer 1H Drawer 3H Sample Handler Assembly 2pos 6port Injection Valve VICI Needle Assembly Needle Port Assembly Station Flush head 500 uL High Pressure Needle Seat 0 12 mm PEEK Sample Loop Flex 20 uL right red coded Sample Loop Flex 40 uL right green coded Sample Loop Flex 100 uL right blue coded Baseplate Assembly 1200 Infinity Series M ultisamplers User Manual 203 10 Parts for Maintenance and Upgrade or Options Peristaltic pump Metering device Dummy drawer Needle assembly Needle seat Injection valve Figure 32 Main user accessible assemblies standard a ELLI SSV piezo pump amp a Boy on L Flush head Metering device Dummy drawer Needle assembly amp Needle seat Injection valve i Figure 33 Main user accessible assemblies multiwash 204 1200 Infinity Series M ultisamplers User Manual Parts for Maintenance and Upgrade or Options 10 Hotel Drawer Hotel Drawer Item p n Description 1 G7167 60021 Drawer 1H 2 p k including 2 G4267 60206 Sample Container 2 G7167 60020 Drawer 2H including 2 G4267 60205 Sample Container 3 G7167 60022 Drawer 3H 2 p k including 2 G4267 60205 Sample Container G4267 60024 Dummy Drawer not shown Figure 34 Hotel drawer 1200 Infinity Series M ultisamplers User Manual 205 10 Parts for Maintenance and Upgrade or Options Analytical H
130. ribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces There is no special interface for this module 242 1200 Infinity Series M ultisamplers User Manual Hardware Information 12 Interfaces ERI Enhanced Remote Interface ERI replaces the AGP Remote Interface that is used in the HP 1090 1040 1050 1100 HPLC systems and Agilent 1100 1200 1200 Infinity HPLC modules All new 1200 Infinity II products using the FUSION core electronics use ERI This interface is already used in the Agilent Univer
131. riven by a high speed hybrid stepper motor During the sampling sequence the valve unit bypasses the Multisampler and connects flow from the pump to the column directly During injection and analysis the valve unit directs the flow through the Multisampler which ensures that all of the sample is injected onto the column and that the metering unit and needle are always free of sample residue before the next sampling sequence begins The Cooling Control of the vial plate temperature in the Multisampler is achieved using an additional Agilent Sample Cooler module The sample cooler is a micro compressor based refrigerator A fan draws air from the central workstation above the sample container of the Multisampler It is then blown through the fins of the cooling module There it is cooled according the temperature setting The cooled air enters the Sampler Hotel through a recess underneath the special designed base plate The air is then distributed evenly through the Sample Hotel ensuring effective temperature control regardless of how many sample containers are in the drawer In cooling mode condensation is generated on the cooled side of the Sample Cooler This condensed water is safely guided into a waste bottle for condensed water located underneath the working bench 1200 Infinity Series M ultisamplers User Manual 15 1 Introduction Multisampler Principle 16 The movements of the Multisampler components during the sampling sequence
132. rmation LAN configuration Safety and related information Agilent Technologies 2014 Printed in Germany 12 2014 Er1BF 30001 G7167 90001 Rev B os Agilent Technologies
133. rument showing the not ready condition Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation 1200 Infinity Series M ultisamplers User Manual Error Information 7 Lost CAN Partner Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Sugge
134. s from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUTDOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as standard TTL levels 0 V is logic true 5 0 V is false 1200 Infinity Series M ultisamplers User Manual 241 12 Hardware Information fan out is 10 input load is 2 2 kOhm against 5 0 V and output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Table 17 Remote Signal Dist
135. s how resolution can be increased by examining the following points Optimize selectivity Smaller particle size packing Longer Columns Shallower gradients faster flow Resolution between two peaks is described by the resolution equation Rs l Jn amp 1 k 1 4 a k where R resolution N plate count measure of column efficiency a selectivity between two peaks ky retention factor of second peak formerly called capacity factor The term that has the most significant effect on resolution is the selectivity a and practically varying this term involves changing the type of stationary phase C18 C8 phenyl nitrile etc the mobile phase and temperature to maximize the selectivity differences between the solutes to be separated This is a substantial piece of work which is best done with an automated method development system which allows a wide range of conditions on different columns and mobile phases to be assessed in an ordered scouting protocol This section considers how to get higher resolution with any chosen stationary and mobile phases If an automated method development system was used in the decision on phases it is likely that short columns were used for fast analysis in each step of the scouting The resolution equation shows that the next most significant term is the plate count or efficiency N and this can be optimized in a number of 1200 Infinity Series M ultisamplers Us
136. sal Interface Box 2 UIB2 ERI Description The ERI interface contains eight individual programmable input output pins In addition it provides 24 V power and 5 V power and a serial data line to detect and recognize further add ons that could be connected to this interface This way the interface can support various additional devices like sensors triggers in and out and small controllers etc Figure 49 Location of the ERI interface example shows a G7114A B VWD 1200 Infinity Series M ultisamplers User Manual 243 12 Hardware Information 244 Pin Enhanced Remote D Sub female i5way user s view to connector es soi Avz amp ol Avze GNSod amp 90 soi anod amp roi AS ansa coi zoi d TOI 9 woJd3MT Olo N DD OT FP UJN p p j l l a l a l a oO FP Wi NY FF oO 10 1 START REQUEST 10 2 STOP 10 3 READY 10 4 POWER ON 10 5 NOT USED 10 6 SHUT DOWN 10 7 START 10 8 PREPARE 1 wire DATA DGND 5 V ERI out PGND PGND 24V ERI out 24V ERI out 10 Input Output Lines Eight generic bi directional channels input or output Same as the APG Remote Devices like valves relays ADCs DACs controllers can be supported controlled 1 Wire Data Future Use This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI device The firmware can detect the connected
137. se it for later transport of the module 58 1200 Infinity Series M ultisamplers User Manual Using the Module 3 If the multisampler with a sample cooler needs to be shipped to another location via carrier ensure The two modules are shipped in separate boxes The Sample handler of the multisampler is parked see Park Robot in Agilent Lab Advisor online help for more information The sample containers vial trays are removed from the sample hotel The condensed water inside of the sample cooler is removed 1200 Infinity Series M ultisamplers User Manual 59 3 Using the Module Transporting the M ultisampler with a Sample Cooler Installed 60 1200 Infinity Series M ultisamplers User Manual 1200 Infinity Series Multisamplers User M anual 4 Preparing the module Leak and Waste Handling 62 Preparing the Multisampler 64 Solvent Information 66 Recommended Mats and Vials 71 Capillary Color Coding Guide 73 Installing Capillaries 74 Setting up the Autosampler with Agilent Open Lab ChemStation 77 Control Settings 81 Method Parameter Settings 82 Module Configuration View 85 This chapter explains the operational parameters of the module ott Agilent Technologies 61 4 Preparing the module Leak and Waste Handling WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these su
138. ser Interface Within the M ultisampler user interface there are active areas If you move the mouse cursor across the icons tray EM F button the cursor will change and you may click on the icon to 1 Turn on off the autosampler 1 2 Configure the sample hotel T 3 Get the status of the EMF Early M aintenance Feature 5 00 ul 4 Cooling Temperatur P1 A 7 Current instrument information on Injection volume Sample location 1200 Infinity Series M ultisamplers User Manual 79 4 Preparing the module f Table 8 The Autosampler User Interface Injector Program Identify Device Home AF Switch on Tray Ilurenation Auto clean Prime Drawer Configuraton Right Capillanes Reference Vial Bar gt Assign Wellplates A right click into the Active Area will open a menu to Show the Control User Interface special module settings Show the Method User interface same as via menu Instrument gt Set up Instrument M ethod gt Setup G7167B Injector Program When you activate a pretreatment injector program it replaces the standard injection cycle Identify Device Home All Switch on Tray Illumination Auto Clean Prime Drawer Configuration Changing the load capacity of the Sample Hotel Right Capillaries Reference Vial Bar Assign Wellplates Wellplate Configuration same as click on the Tray icon 80 1200 Infinity Series M ultisamplers User Manual Preparing the module
139. sted actions 1 Defective leak sensor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative 1200 Infinity Series M ultisamplers User Manual 113 7 Error Information Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the Power Please contact your Agilent service Switch board representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being pinched by a metal component Please contact your Agilent service representative Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is ge
140. t and the separation looks the same but happens in half the time Recently published research has shown how a shorter STM column at temperatures above 40 C can generate higher peak capacity than a longer STM column by virtue of running it faster Refer to Petersson et al J Sep Sci 31 2346 2357 2008 Maximizing peak capacity and separation speed in liquid chromatography 100 1200 Infinity Series M ultisamplers User Manual Optimizing Performance 5 How to Achieve Higher Sensitivity The sensitivity of a separation method is linked to the choice of stationary and mobile phases as good separation with narrow peaks and a stable baseline with minimal noise are desirable The choice of instrument configuration will have an effect and a major impact is the setup of the detector This section considers how sensitivity is affected by Pump mixer volume Narrower columns Detector flow cell Detector parameters In addition the discussion on detector parameters also mentions the related topics of selectivity and linearity Columns Sensitivity is specified as a signal to noise ratio S N and hence the need to maximize peak height and minimize baseline noise Any reduction in peak dispersion will help to maintain peak height and so extra column volume should be minimized by use of short narrow internal diameter connection capillaries and correctly installed fittings Using smaller inner diameter columns should result in high
141. taltic pump delivers 5 mL min which means the flush port volume is completely refilled with fresh solvent in 7 s If the flush port is selected the user can set how long the outside of the needle is to be washed with fresh solvent This can last two or three seconds in routine situations where carryover is less of a problem and 10 20 s for more complete washing It is recommended that washing the outside of the needle in the flush port should be standard procedure to avoid contaminating the needle seat If the needle seat becomes contaminated it will have to be back flushed In the standard setup it must be done by manually changing the flow connections to clean it In this standard configuration the task can be done automated by using the Flexible Cube module If you have installed the Multisampler with Multi Wash option the flushport will be primed with a micro piezo pump This pump can choose between 3 different wash solvents The flush port and its solvent delivery pump and tubing should be regularly flushed to ensure the lowest carryover For example before using the system each day prime the flush pump for three minutes with appropriate solvent When other measures have failed to eliminate carryover it might be that analyte is sticking inside the injector valve With auto clean feature in the CDS system the injector valve can be set to make additional switching movements to clean out the flow path in the valve if problems occur
142. tenance Parts required p n Description 5067 6127 Blank Nut SL 1200 Infinity Series M ultisamplers User Manual 119 8 Test Functions and Calibration System Pressure Test 1 Run the System pressure test with the Agilent Lab Advisor for further information see Online Help of user interface General Limits Signals Test Name System pressure test for Aladdin Description Preliminary system pressure test for Aladdin Module G4220A LP00000003 Approx Time Not defined Status Passed Result Prepare pump pressure test System leak 2 1 bar Enter the test pressure Flush the system System checking leak rate of pump Insert blank nut System checking leak rate of system Evaluate results Restore system configuration SEN NNN NaSGN Baa I N Es ON a Figure 22 System Pressure Test Result Pump head leak test Enter the pressure at which the test will be executed Figure 23 System Pressure Test Dynamic pressure input 120 1200 Infinity Series M ultisamplers User Manual System Pressure Test Evaluation Test Failed Probable cause 1 Damaged blank nut poorly shaped from over tightening 2 Pump leakages 3 Loose or leaky fittings Autosampler leakages u A Themostatted Column Compartment valve leakages Test Functions and Calibration 8 Suggested actions Before investigating any other possible sources of failure make sure that the blank nut you are using is in a good c
143. the fitting hand tight 2 CAUTION Blockages gt Do not overtighten the fitting A quarter turn should be sufficient 2 Then usea 1 4 inch wrench to tighten the fitting of the loop capillary 4 Click the sample loop cartridge in the designated location and keep the right orientation 1200 Infinity Series M ultisamplers User Manual 185 9 Maintenance Installing the Sample Loop Flex 5 Install the shorter capillary of the sample loop cartridge to the analytical head 6 Pinch and reinsert the needle assembly and the connected sample loop capillary into the z arm coupler Check the tension of the loop capillary This must be forced and guided to the hydraulic box to prevent it from being caught by the Z drive 7 Close the front door Eal m In the Instant Pilot close Change needle seat OR In Agilent Lab Advisor software Change needle loop Click NEXT and wait until the needle is in the needle park station Click Back to leave the M aintenance window NOTE If you need an autoreferencing step included you must choose the change needle procedure 186 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Replace the Dummy Drawer Replace the Dummy Drawer Optional Configurations Table 13 Overview on optional configurations examples for uniform types 1H 2H 3H Dummy Drawer gt gt Delivery Sta
144. the piston carefully into the housing of the head 6 Reinstall the complete analytical head with the actuator body and the seal housing For proper installation check the correct position of the tag 1200 Infinity Series M ultisamplers User Manual 165 9 Maintenance Install the Metering Seal 7 Fixthe analytical head by pushing 1 and rotating 2 via 8 Reconnect the capillaries twist and lock bayonet mechanism 166 1200 Infinity Series M ultisamplers User Manual Maintenance 9 9 Close the front door In the Instant Pilot exit the maintenance mode and select Change metering device function OR In Agilent Lab Advisor software system screen exit Service amp Diagnostics Tools gt Maintenance Positions gt Change Metering Device click End and wait until the metering device is in Home position Eal 10 Perform a pressure test m _ 1200 Infinity Series M ultisamplers User Manual 167 9 Maintenance Replace the Peristaltic Pump Cartridge When Tools required Parts required OR OR Preparations Tubing blocked or broken Description p n Description 1 5065 4445 Peristaltic pump with Pharmed tubing default 1 5042 8507 Peristaltic pump cartridge silicone tubing 1 5042 9952 Peristaltic pump with Chemsure tubing Remove the inlet filter of the solvent bottle which guides the solvent to the peristaltic pump to avoid syphoning effe
145. tical head assembly 900 uL 208 1200 Infinity Series M ultisamplers User M anual Parts for Maintenance and Upgrade or Options 10 Flush Head Assembly 500 pL ltem p n G4267 60049 G4267 60491 5023 2473 G4267 60482 5067 5918 m 1410 1881 G4267 60432 5067 5919 G4267 60451 G4267 60452 oOo ae N Y OA oT A UWV N m 1200 Infinity Series M ultisamplers User M anual Description Flush head 500 uL Flush Head Assembly 500 uL Sealing Plate 500 uL Cylinder Assembly 500 uL Seal 500 uL Screw Bearing Sleeve 8 mm ID 10 mm OD 10 mm LG PI Spring Adapter Assembly Piston Assembly 500 uL Pump Valve IN Pump Valve Out 209 10 Parts for Maintenance and Upgrade or Options Flush Head Assembly 500 pL Figure 38 Flush head assembly 500 uL 210 1200 Infinity Series M ultisamplers User Manual Injection Valve Assembly ltem p n 5067 4232 1 5068 0019 2 5068 0197 3 5068 0198 5500 1159 5067 4650 5500 1157 5067 6127 Parts for Maintenance and Upgrade or Options 10 Description 2pos 6port Injection Valve VICI Stator screws Stator head Rotor Seal for VICI Injection Valve PAEK Capi lary ST 0 17x100 SX 2 3 Metering Device to Injection Valve Capi lary ST 0 12 mm x 150 mm SL SX Pump to sampler Capi lary ST 0 12 mmx500 mm Sampler to column compartment Blank Nut SL For the VICI Valve SL SX fittings are mandatory 1200 Infinity Series M ultisamplers User Manu
146. tions in the flow path 136 1200 Infinity Series M ultisamplers User Manual Maintenance 9 Removal and Installation of the Front Door When Tools required Parts required Preparations CAUTION If the front door is defective or the hinge are damaged Description Flat screwdriver p n Description 5067 5415 Door Assy Finish any pending acquisition job and return any plate on the workspace back to the hotel For detailed information on position of the magnets refer to Magnets on page 44 Magnetic fields Magnets produce a far reaching strong magnetic field You can damage for example televisions laptops computer harddisks credit cards magnetic cards may be damaged as well gt Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields Heart pacemakers Magnets may derogate the function of heart pacemakers and implanted defibrillators Heart pacemakers may switch to the test modus which may cause malaise Defibrillators may malfunction gt Bearers of heart pacemakers or implanted defibrillators must stay off at least 55 mm from the magnets 1200 Infinity Series M ultisamplers User Manual 137 9 Maintenance Removal and Installation of the Front Door 1 Open the front door 2 Press the release buttons and pull the front door out oP U 138 1200 Infinity Series M ultisamplers User M anual Maintenance 9 Re
147. tom sensing feature is used the bottom of the plates and vials must resist the needle M ake sure that the material supports this feature NOTE 72 1200 Infinity Series M ultisamplers User Manual Preparing the module 4 Capillary Color Coding Guide Capillary Color Coding Guide Description Metric M4 0 8 mm Port id Metric M3 1 6 mm Port id Fused silica in contact with solvent PEEK in contact with solvent The type gives some indication on the primary function ike a loop or a connection capillary The material indicates which raw matenal is used The fitting left right indicate which fitting is used on both ends of the capillary At a glance color coding keys The calor of your capilayw lbelpyou Color coding key for Agilent capillary tubing nekaa Internal Diameter in mm Color code 03 0 50 Bone White Tip As you move to smaller valume high efficiancy columns you ll want to use narrow id tubing as opposed to the wider id tubing used for conventional HPLC instruments Figure 11 Syntax for capillary description 1200 Infinity Series M ultisamplers User Manual 73 4 Preparing the module Installing Capillaries For correct installation of capillary connections of the multisampler it s important to choose the correct SL SX fittings see Capillary Color Coding Guide on page 73 Thread groove too short fot Vici valves 5065 4454 Fitting screw
148. tus G7167 60020 G4267 60024 iz i 1x 3x Up to 8 single height G7167 60021 drawers 8x 16 positions Shallow wellplates and MTP M ax Sample capacity 1536 6144 samples 96 Shallow Wellplates 384 MTP Up to 4 Dual Height drawers G7167 60020 8 positions Vials 2 mL deep 4x well plates M TP Eppendorf M ax Sample capacity 432 3072 samples 2 mL Vials 384 MTP Up to 2 Drawers Triple G7167 60020 G7167 60022 Height 1x 2x 4 positions 2H or 2 1H option left over Vials 6 ml deep well plates MTP Eppendorf M ax Sample capacity 60 216 1536 samples 6 mL Vials 2 mL Vials 384 MTP 1200 Infinity Series M ultisamplers User Manual 187 9 Maintenance Replace the Dummy Drawer NOTE Mixed configurations are possible for example 1x3H with 1x2H and 3x1H drawer All positions in the Sample Hotel must be filled either with dummies or drawers The drawers must be installed from bottom to top 188 1200 Infinity Series M ultisamplers User Manual Maintenance Installing and Replacing of Drawers Upgrade Drawer Kit Tools required Parts required Description Screwdriver p n G7167 60020 G7167 60021 G7167 60022 Description Drawer 2H Drawer 1H Drawer 3H Before you start the new drawer installation you have to remove the lower drawer 2H draw er default configuration from the Sample Hotel For the best cooling performance avoid the installation of a 3H drawer in the lowest
149. type of device automatically and update information in the device if required 1200 Infinity Series M ultisamplers User Manual Hardware Information 12 5V Distribution Future Use Available directly after turn on oft the hosting module assures that certain base functionality of the device can be detected by firmware For digital circuits or similar Provided 500 mA maximum Short circuit proof with automatic switch off by firmware 24V Distribution Future Use Available by firmware command defined turn on off For devices that need higher power Class 0 0 5 A maximum 12 W Class 1 1 0 A maximum 24 W Class 2 2 0 A maximum 48 W e Class depends on hosting module s internal power overhead If a connected device requires more power the firmware detects this overcurrent detection and provides the information to the user interface Fuse used for safety protection on board Short circuit will be detected through hardware 1200 Infinity Series M ultisamplers User Manual 245 12 Hardware ein S g the 6 bit Configuration Switch Setting the 6 bit Configuration Switch The 6 bit configuration switch is located at the rear of the module with FUSION electronics Switch settings provide configuration parameters for LAN and instrument specific initialization procedures All modules with FUSION electronics Default is ALL switches DOWN best settings Default IP address for LA
150. ule can lead to personal injuries e g Shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Do not use the sample cooler if it is not operating correctly or has been damaged Disconnect it from the power supply and call your local service center gt Remove the power cable from the module before opening the cover gt Do not connect the power cable to the module while the covers are removed gt Ifthe sample cooler is disconnected from the power supply you should wait for at least five minutes before switching on the compressor CAUTION Damaged electronics gt To avoid damages of the electronics of the module make sure the power cords are unplugged before disconnecting or reconnecting the multisampler to the sample cooler cables 1200 Infinity Series M ultisamplers User Manual 53 3 Using the Module Installing the Sample Cooler 7 Connect power cable and signal data cable CAUTION Damage to the cables gt Do not bend or pinch the cables gt Fitin the sample cooler perfectly 8 Slide in the whole unit 54 1200 Infinity Series M ultisamplers User Manual UsingtheModule 3 nstalling the Sample Cooler 9 Tighten the 4 screws which holds the sample cooler unit in place CAUTION Routing of the condensation tubing Proper routing of the condensation tubing is critic
151. ule via CAN The detector DAD M WD FLD VW D RID is the preferred access point for control via LAN The inter module communication is done via CAN CAN connectors as interface to other modules LAN connector as interface to the control software RS 232C as interface to a computer 238 1200 Infinity Series M ultisamplers User Manual Hardware Information 12 USB Universal Series Bus as interface to a computer REMOTE connector as interface to other Agilent products Analog output connector s for signal output Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MW D FLD VW D RID is in the system the LAN should be connected to the DAD MW D FLD VW D RID due to higher data load If no Agilent detector is part of the system the LAN interfa
152. use an ellbow connector and avoid siphoning effects of the drainage 56 13 Ensure the power switch on the front of the module is OFF switch stands out CAUTION Damage to the sample cooler gt Wait at least 30 min before switching on the compressor of the sample cooler gt This allows the refrigerant and system lubrication to reach equilibrium 1200 Infinity Series M ultisamplers User Manual Using the Module Installing the Sample Cooler 3 14 Connect the CAN interface cables to other modules in the system see section Recommended Stack Configurations in the technical note Use of M ultisampler in Mixed Configurations or the service manual 15 If required connect additional interface and control cables to the autosampler see section Recommended Stack Configurations in the technical note Use of M ultisampler in Mixed Configurations or the service manual Refer to the documentation of the Agilent 1200 Infinity Series Instant Pilot or ChemStation for LC for more information In an Agilent 1290 Infinity or 1260 Infinity system the individual modules are connected by a CAN cable The Agilent 1200 Infinity Series Instant Pilot can be connected to the CAN bus at any of the modules in the system If an Agilent detector is part of the system usually the LAN connection should be at the detector For more information about connecting the instant pilot or control software refer to the respective user ma
153. y catching all internal liquid leaks guiding them to the leak sensor for leak detection and passing them on to the next module below if the leak sensor fails The leak sensor in the leak plane stops the running system as soon as the leak detection level is reached Solvent and condensate is guided through the waste channel into the waste container e from the detector s flow cell outlet from the Multisampler needle wash port from the Sample Cooler condensate e from the Seal Wash Sensor from the pump s Purge Valve or Multipurpose Valve The waste tube connected to the leak pan outlet on each of the bottom instruments guides the solvent to a suitable waste container 1200 Infinity Series M ultisamplers User Manual Introduction 1 Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports 5043 1221 for optimal and safe waste disposal If you decide to use your own waste solution make sure that the tubes don t immerse in the liquid 1200 Infinity Series M ultisamplers User Manual 25 1 Introduction System Overview Leak and Waste Handling in a Mixed Configuration Leak and Waste Handling in a Mixed Configuration The leak pan outlet of the upper module must be vertically positioned above the leak tray of the lower module Detector Oven Sampler For the lowest module the central waste is guided directly into the waste container Pump Figur

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