SAIO - User Manual - P.S.Elettronica
Contents
1. Ready Sieroproteine 29 04 2012 10 45 19 Picture 2 12 Change Tracks On the Edit Traces screen both the graph and the relative values of the fractions for each sample are illustrated At the top left there 1s also the list of the analyzed samples On the same screen as in the previous one you can enter or edit data of each sample On the graph window it is possible to insert any necessary changes such as inserting moving and deleting minimum changing the base line and zooming by clicking on the squared button on top right of the graph window see Picture 2 13 28 Grafico OT Color Patterns w Subtract Baseline Minimum Compute Export Gamma Correction oom slider List Picture 2 13 enlarge image NOTE For more details on the changes effectively the tracks you can refer to Section 4 2 Edit Traces J Once made final changes of the histograms can be traced to the press of selecting the Print window at the top next to the Change Tracks see Picture 2 14 29 ED Aries Rel Mar 13 2012 22 20 29 File Features 7 Change Tracks Print Cognome Nome Fi UPD imig 7 ri IRA LI S eronroTrelne ie AA lt i nt 745 03 02 20 12 Si roproteine EA OE ea te On Oe Te Ready Sieroproteine 29 04 2012 10 45 19 Pictrure 2 14 Print Reports You can print the report by clicking the right mouse button on the row and you will see a2. Table 4 Print Preview 55 5 Control Machine Control Machine E Methods Today s data Search Exams El Archive This section of the program allows you to connect SAIO electronics and software to the physical machine the robotics sensors and to the electrophoresis operations Clicking on the menu Control Machine to access the Dashboard Control that allows you to turn on and off the machine to modify the voltage of the chamber during a migration to run a selected process from the list of the services to pause and resume a process already running If an error occurs it 1s possible to go to a next step or stop the process permanently The error of the process will be outlined in a separate control at the bottom of the screen The window also has status indicators full or empty for the levels of the machine that are classified as external levels such as Staining Destaining H20 buffer buffer HB and waste and as internal levels such as staining destaining Imbibition buffer in Migration Chamber m Turn on and Turn off keys to switch on off the machine for the electrophoretic process or to perform other operations selected by the user Fac Process Pause and Restart Process keys to pause or to restart the process Start Process key to start a process selected by the command Process List Continue Process key can be clicked when the proc
3. Add a new fraction group to the current method It is not possible to edit the fraction list and it is fully completed in all its fields The combination of the fraction groups will have the name of the default fraction groups with the current methodology Patient Data To insert modify the patient data Table 2 Change Tracks 4 2 1 Graph Grafico O Picture 4 Graph The chart associated with the patient is the result of the reading performed by the scanning process of the samples The graph displays the minimum values through the vertical lines 48 These minimum levels can be moved using the mouse with a drag and drop operation on the graph The minimum lines can be deleted or added on the graph by double clicking the mouse It 1s possible to open a drop down menu with the right mouse button on the graph in order to make relevant changes see Picture 4 9 Grafico Color Patterns w Subtract Baseline Minimum Compute Export Gamma Correction Zoom slider List Picture 4 9 Drop Down Menu Graph e Color Patterns the patterns of the graph can have the color set up in the definition of the fractions for the current method of work and can take the dotted curve or be transparent see Picture 4 10 Transparent Color Patterns E i Dotted e Stained w Subtract Baseline Minimum Compute Export Gamma Correction zoom slider Picture 4 10 Color P
4. enabled for entering patient data GREEN indicates the slot selected by the mouse with the corresponding data of patients included or to be included RED slot occupied by a patient Selecting a slot with the left button of the mouse it is possible to update patient data in the slot Selecting an occupied slot with the right button of the mouse a drop down menu will appear and it will be possible to remove patient from the slot clicking Delete Patient BLACK Invalid slot Selecting a non valid slot with the left button of the mouse it is possible to clear and disable the data of the patient to the right of the screen Selecting a non valid slot with the right button of the mouse it is possible open a drop down menu with which you can select the Restore Patient to restore the patient in the deleted slot The serum holder contains also the following labels e Status indicates the status of current serum holder The possible values EMPTYING PREPARATION READING PROCESSED and CANCELLED e N serum holder indicates the number of serum holders e Note additional note that can be associated to the serum holder e Number of Slots The number of selected slots e Remainder indicates the number of strips still present in the Smart Card 43 To facilitate the scrolling of the serum holder and consequently the patient detail linked to the selected slot Patient Data It is situated on the right side of the sc
5. Clicking on the Quality Control key you access the windows of Configuration 65 e Results and Graphs that allow the following operations Management of the quality control of the examined samples Report of Quality Control in tabular format Report of the quality control in a graphical format 66 8 Registries It is possible to create a database with all the information related to doctors sectors disease and laboratories le Features Control Machine Methods Arch Today s data E Search Exams Archive Registries Quality Control Restore Layout tab Alarm OFF A AAA E 8 1 Laboratory TOS T F Y F YF Change Tracks Laboratory Diseases Doctor Department Close In this screen it is possible enter all the information about the report The field concerning the description of the Electrophoresis of serum proteins is available only for the reading and it is set up with the method name displayed on the Method Parameters configuration screen see Picture 8 1 67 File Features 7 00006 ca Pos X Y TextLine 1 Pos X Li Text Line 2 Pos X Li Text Line 3 xf 13540 GAMMADIAGNOSTIC 175 MEDEA 120 Marmolar Street 20 T Picture 8 1 Changing the header Please find below description for each particular field on this screen Previous laboratory detail and Following laboratory detail keys
6. End position on the graph double m_FractionRatio Relation value A G double m_RatioNormMin Relation Value A G min double m_RatioNormMax Relation Value A G max double m_FractionVal Relation Value double m_ValueNormMin absolute value Min double m_ValueNormMax absolute value Max DATAFRACTION LPDATAFRACTION define SIZE_DATAFRACTION sizeof DATAFRACTION struttura dati bitmap grafico typedef struct Int m_Size size file bitmap of the graph char m_FileData 12288 data file max 12 KB DATAGRAPH LPDATAGRAPH define SIZE_DATAGRAPH sizeof DATAGRAPH struttura dati bitmap strip typedef struct int m_Size size file bitmap of the graph char m_FileData 3072 data file max 3 KB DATASTRIP LPDATASTRIP define SIZE_DATASTRIP sizeof DATASTRIP 84 struttura dati del risultato del paziente typedef struct DATAPATIENT unsigned long unsigned long char double double double char double double unsigned long unsigned char DATAFRACTION DATAGRAPH DATASTRIP m_Patient dati paziente m_AcquisitionID m_StripPos m_Comment 256 m_NormValRifAG m_NormValRifAG_Min m_NormValRifAG_Max m_UnitOfMeasureAG 32 m_NormValProtTot_Min m_NormValProtTot_Max m_StripLen m_MinTags m_Fractions 12 m_GraphBitmap m_StripBitmap RESULTPATIENT LPRESULTPATIENT define SIZE_RESULTPATIENT endif DScanSocketData_h sizeof RESULTPATIENT Id lettura posi
7. Save patient and graph save a data modification The Clean button reset the screen parameters The Associate a comment to a patient associate a comment to the comments list of the patient The Disassociate patient comment disassociates a comment from a patient The Print patient report key visualizes the print preview of the report The Changeable patient data key can modify the patient data Once pressing this button it will not be possible to change the detail anymore The No changeable patient data key indicates that it is not possible to change the data since the report has been already delivered It is only possible to read the data This is the tree command the collect all the patient of the day This command located in the middle left part of the screen collects the fraction values into a numerical format It will be automatically entered when you modify the graphs of the reading The fraction list becomes more editable with the Factor column that can change the fraction value The list of the fractions will be editable for the Fractions 47 Int Rif Int Rif Ratio A G and Total Proteins only when the number of the fractions of the graph will be different from the default number of the current method for the selected patient Otherwise you can add a new fraction group for the current method clicking the button
8. 00 32 0 65 0 52 0 65 0 52 0 65 0 No 2 Alphal 1 00 20 3 0 20 3 0 20 3 0 No Picture 3 6 Normal Values Modification Change the value of the fraction in the appropriate field a checkmark will appear in yellow Clicking this button the value will update Save the changes by clicking on the saving button see Figure 3 7 36 Human Serum from 5 wo Divisor 2 3 4 5 N Fractions Factor Min Max Min Max Min Max Grid ra 3 m La I YA Albumin 400 5200 6500 5200 65 00 51 00 65 00 No AN 2 Alpha 100 20 5 0 20 5 0 20 5 0 No at ph 100 70 230 70 70 13 0 No Bata on 8 0 ie 4 No PEN Samir a 20 e Ratio A G 1 00 2 10 1 00 1 00 2 10 Picture 3 7 Save the updated fraction An important parameter is the factor placed in the first right column containing the names of the fractions The factor can increase or decrease the numeric read value for each fraction This operation is performed essentially in the calibration phase of the machine by using a known sample or a control sample Fractions Values values can be expressed in percentage or in g dL Samples Category the types of patients can be chosen in the adjacent field human or animal or it is possible to choose the group fractions see Picture 3 8 Picture 3 8 Sample Category Sum of Fractions it determines the fractions on the basis of the calculation of the ratio A G in particular to the dividend i
9. Necessary content to run 400 serum proteins tests 223 pieces of strips supported on Dry Plates 1x100ml buffer Tris hippurate 1x200ml Staining Ponceau S ready to use 2x250ml Destaining Concentrate 20 X 20 Applicator papers 70 x 13 mm 6 Strip drying papers 92 x 47 mm 4 Migration chamber floaters 70 x 20 mm Product identification and intended use The reagents and accessories for the clinical electrophoresis diagnosis of serum proteins are intended to be used to research of disproteinemie and to quantify albumin alpha 1 alpha2 Beta and gamma globulins The reagents should be used only for the purpose that has been defined above and by a qualified laboratory Use fresh serum as specified in laboratory procedures and in conformity with the guidelines Avoid the use of plasma because the fraction of fibrinogen may disturb the interpretation of the gamma area Do not use samples hemolyzed or containing cells Reagents preparation Concentrate buffer Pour about 80 ml of distilled water in the bottle 13 Ponceau S destaining ready to use Concentrate destaining 20 X Pour about 5 liters of distilled water in the bottle Keep strip and reagents to a ambient temperature 15 30 C The best before dates are indicated on the labels Once opened the reagents are stable and can be used until expiration date if closed immediately after use and protected from direct sunlight avoiding the evaporation Warning If black spo
10. Picture 6 2 Setting Filters 60 File Features Delete Exams Delete serum holder A RSU La di 4 w UL VY H N Data Acc Cod Acc Metodica Cognome Nome Eta Sesso Tipo Paziente 2 02 02 2012 Sieroproteine Umano 4 02 02 2012 Sieroproteine M Umano 6 Dr Sieroproteine Umano 8 02 02 2012 Sichaprotenie M nario Picture 6 3 Filters Delete Patients Select Patient key allows selecting all the patient of the list Double clicking deselects them all Delete Patients key deletes the patient from the database The Clean key resets the screen parameters Search patient in the archive key displays the window for the search of the patient in the archive Lista Pazienti The list contains the patients of the day To print a patient from the list click on it with the right button of the mouse The selected patient will be marked with the check click again to deselect it Table delete Exams Patient 61 6 2 Delete Serum Holder File NRE sn la nate gie ede Pri eri sei elia p Archive serums holder E gt Sieroproteine E a eave 19 11 6 Picture 6 4 Delete Serum Holder Clicking on the Search serum holder in the archive button the last entered serum holder will be searched and then you can delete it See Picture 6 4 ES Archive serums holder S E Sieroproteine a E 28 03 2012 Siero mem 20 03 201
11. Preview With this operation it is possible to print reports of patients On the header of the report there is the name of the laboratory where the tests have been performed Personal Data Laboratory in the central part of the report appear some patient data the graph and the image of the migration on the footer the comments and the signature of the analyst Please find below description for each particular field on this screen See Picture 4 17 Anteprima di Stampa PS FIETTRONMICA SI PALOMBA VINCENZO LADORA TORTO EY GOLA LADO VIA MOANA 2 TRAVERSA 20 POMPEI NA Tel ORT sore SIEROPROTEINE 4 VERDI GIORGIO Fa 57 Data Aci 01 11 2010 Codre Acces 585 Frazioni ES Tat KIE 3o Albumina 52 00 66 00 Alfal 2 00 5 00 Alfaz 6 00 11 00 Beta 2 00 14 00 Gamma 10 00 20 00 Fagoorte AAS Rif 1 20 2 10 Prptame Total gi Rif 6 50 8 50 Lanatista DE ROSSI Picture 4 17 Print Preview Please find below description for each particular field on this screen 54 Field Name Description Clase Close key to close the Print preview Print key to start the print of the patient reports These keys scroll the pages of the report print previews Goto key to access to a specific print preview page q 1 65 val printing Any modification will appear only on the monitor The combo is used to set the zoom percentage of the page before
12. and Next key you can scroll through the results of the various patients An interesting feature of SAIO is the printing it produces a report that apart from the graph of the curve and the numerical results also contains the image of migration the personal data and comments The resulting printing can then be delivered directly to the patient with no need to attach other documents such as reports or images of migration 19 2 1 Tutorial Before the detailed description of the operations offered by SAIO the user may consult the following tutorial for an overview of the operational program a Start the machine double clicking on SAIO icon at the center of the Desktop b When initializing SAIO a splash page will appear see Picture 2 1 mm 9 CUT Ca VETERINARY Picture 2 1 Splash page 20 a After initialising stage the Today s Data will appear as first page see Picture 2 2 i Taki ae ett a 23 ae wes a al gt es Features F sy Archive serum holder today e E CI re O il 27 04 2012 v 27 04 2012 v Control Machine A Methods E Today s data A Search Exams 9 Archive Picture 2 2 Today s Data SAIO utilises a programme with two menus the first one appears on the top of the application and 1s a dropdown menu see Picture 2 3 In the dropdown menu you can select some useful features e From the File menu you can select the Select language command to access to
13. check mark appearing on the left of the numbered line otherwise you can choose to select all the read samples by clicking on the symbol with a tick see Picture 2 15 Cognome Sesso Tipo Paziente Picture 2 15 Print all selected samples 30 k Selected the samples to be printed by clicking on the yellow Printing icon 4 Print Preview window will appear 2 Anteprima di Stampa P S ELETTROMICA DI PAL MBA VICENZA LADORA TORTO DI COMA LADO Mi MOL 2 TRAVERSA 20 POMPEI NA Tel OBIB633760 SIEROPROTEINE 4 VERDI GIORGIO Fa 57 Data Aci 01 11 2010 Codice Acces 585 Frazioni Ez Ior EiL fo Albumina Sii 52 00 66 00 Alfal z Er 2 00 5 00 Alfaz GF 6 00 11 00 Beta 1465 2 00 14 00 Gamma 1765 10 00 20 00 Fagoorte AAS 128 Fif 11 20 2 10 Preje Total gi 690 Fif 6 50 3 50 l analista DR ROSSI Picture 2 16 Print Preview D Print the report clicking on the report icon 31 3 Methods PI control Machine Fy Methods Today s data Search Exams El archive In this section of the program the user can choose the method for the analysis of samples Clicking on the menu Methods to access to Configuration Parameters Definition Fractions and Definition Comments these windows allow the following operations e Choosing a method from the default ones by selecting it with the mouse in the window List of methods section on the left side of the screen and setting it as current m
14. curve which can be changed by the user with interaction of the mouse with the graph the numerical data of the fractions which can be automatically updated according to the curve changes In this screen there is also a small window underneath the fractions dedicated to comments where it is possible to enter a new comment or edit an existing one To access the details of a new sample use Previous patient details and Next patient details keys ate ies at 1 LI Slotn 1 ne PT or click directly on a control sample from the Archive on the left side of the window Print one or more samples reports selecting them from the list with the right button of the mouse or selecting all of them pressing the Select Patient and Print patient keys 40 4 1 Managment Serum Holder Management serum holder Archive Archive serum holder today E Change Tracks The operations that can be made in this screen are inserting a new serum holder of the day refilling each slot with patient data and starting the process of reading samples The application can be configured with a 16 slots serum holder See Figure 4 2 Port serum 0 0 O O JO O UT E E E E gt UNOS it Picture 4 2 Serum Holder 16 samples Please find below description for each particular field on this screen Field Name Description The Previous serum holder details and Next serum holder details are used to scrol
15. inside the machine The left column shows the status of the liquid levels At the center of the field called Migration check the values of voltage the current migration and the remaining time for migration The Machine Status indicates the status of the machine The List of services allows the user to perform routine maintenance of the machine 26 RY Aries Rel Sep 17 2013 20 File Funzionalita Livelli Esterni Y Scarico Colorante Decolorante W H20 U Tampone Livelli Interni Y Colorante Decolorante Y Imbibizione Camera Mi Servizi Utente Valvole 26 09 2043 21 38 28 Picture 2 11 Control Machine j At the end of the electrophoretic process after the densitometric reading of the strip you can see the traces going back to Current data gt Edit traces see Picture 2 12 2l File Features Management serum holder a Change Trac Tracks E Print Print Archive PS E 02 02 2012 Al 96 lt P ME 1 James pass B a te 57 04 2012 3 D Annunzio Gabriele ila ieroproteine i BM Il Lo i 6 7 8 Frazioni Fat ImtRif g dL Int Rif g dL Albumina 56 9 1 00 57 065 0 0 00 3 12 5 20 27 1 00 2 5 5 0 0 00 0 18 0 40 115 4 2 010 042 080 Proteine Totali g dL 1 32 Rif 1 002 10 0 00 Rif 5 00 8 20 IL TL Control Machine E methods _ J E today 5 data El search Exam Exams ms ahe
16. one or more samples selecting them from the list with the mouse clicking or select them with the Select Patient button 64 File Features Advanced Search Print a WE 3 4 CA y JQ Date Acc Acc Code Method Surname Name Aqe Sex Patient Type 2 17 Da 2012 ne Umano 17 092012 Slerapratelne Umana 5 osae Sieroprotee ano G 17 09 2012 Sieroproteine Umano TO osama Sieropiateie tno 3 17 09 2012 Sieroproteine M Umano A MO Umano 2 Da 08 2012 Sieroproteine M Umano 4 08 08 2012 Sieraprateine M Umano ee m G 08 08 2012 Sieroproteine M Umano E tno 3 08 08 2012 Sieroproteine Umano o oaoa A MO Umano 10 0808 2012 Sieroproteine Umano E ano 12 03 08 2012 Sieroproteine Umano FS ooa pe man 14 08 08 2012 Sieroproteine M Umano mme m m mmm me Picture 7 3 Print Patient 7 2 1 Registries It is possible to create a database with information regarding doctors sectors diseases and laboratories Clicking on the Registries button you access the windows of Laboratory Doctors Sectors Diseases that allow the following operations Management of headers for the report printings of the laboratories Management of the patient s pathology Management of the doctor s data Management of the sectors Tae Quality Control It is possible to create a database with information regarding the quality control of the examined samples
17. scroll the list of the laboratories names Set the laboratory as printing heading key sets the selected header as a referential one Add new laboratory key adds a new heading into the list Remove laboratory keys deletes the selected heading of the laboratory Save the laboratory key saves the selected heading of the laboratory 68 Clean key resets the screen parameters Printer Properties displays a drop down menu O OS Select Font Font in Italic Underline Font w GAMMA DIAGHC Font Color Print Preview This menu manages the type of font of character italics bolt underlined of color and the print preview Table 5 Laboratory 69 8 2 Diseases le Features 7 E tension E Diseases C Doctor Department 0009 Diseases List Morbillo Picture 8 2 Disease Please find below description for each particular field on this screen Field Name Description Previous diseases detail and Following diseases detail keys scroll the list of diseases Add new disease key enters a new disease in the list Remove Disease key deletes the selected disease Save disease key saves the selected disease in the list Clean key resets the parameters of the screen Table 6 Diseases 70 83 Doctor In this screen it is possible to manage the doctors data File Feature
18. sizeof unsigned char NUM_PAZIENTI SIZE_DATAPATIENT 0 NUM PAZIENTI numero pazienti da inviare 1 n DATAPATIENTINUM_ PAZIENTI strutture dati dei pazienti da inviare in the 0 position will be insert the number of sent patients and from position will be insert the data structure such as DATAPATIENT in a sequence similar to the number of patients unsigned char cSize 4 cSize 0 3 sizeof cResponse The buffer response that will be sent to the SAIO communication channel and it will be the result of the connections of the buffers CSize cResponse On top of the buffer will be copied the actual number of bytes that identifies the data import response Use the file specified in Appendix A to implement the communication buffer between the SAIO and the third parties 10 2 Export Patient The export of patient data to an external database is performed in the feature Current data Change Trace Current data Print Search test Advanced Search and Search Test Print The start of this feature requires the following steps 1 Enter the IP address or the machine name to which send patient data 2 The connection with the machine will start via the communication channel SOCKET_IMP_EXP_PORT 3300 SI 3 The request is sent through the composition of the following buffer data unsigned char cSend 2 NUM_PAZIENTI SIZE_RESULTPATIENT cSend 0 EXPORT_PATIENTS codice di identificaz
19. the desired type the research will start by clicking with the mouse on the key Search Start The result appears in the list Delete one or more samples from the list by selecting them using the right button of the mouse or selecting them all clicking Select Patient and then the Delete Patients e Clicking on the key Search serum holder in the archive the last entered serum holder will be searched The result will be displayed in the Cancellation in the top left part of the screen In order to delete the serum holder press the Delete serum holder File Features E Delete Exams Delete serum holder Date Acc Acc Code Method Sumame Name Age Sex Patient Type Picture 6 1 Delete Exams 59 6 1 Cancella Esami 2 Delete serum holder E Delete Exams Clicking the yellow key Search patient in the archive DI you can find the patients in the database and then delete them During the search the Search Filter window is displayed see Picture 6 2 The search result is displayed in the list Delete one or more samples from the list by selecting them using the right button of the mouse or selecting them all clicking Select Patient and then the Delete Patients see Picture 6 3 File Features i Delete Exams dg ra Filt rch o x E Setting Filters Setting Filters 18 02 2014 w tient Type SS Serum proteins 3
20. the back panel by unscrewing the fixing screws At this stage pay attention when unplugging the cable used for grounding system The manufacturer will provide appropriate instructions and wiring diagrams to the authorized personnel for the maintenance and or repairs The customer should always contact authorized service centres or when possible send the machine to the head office 1 7 Warranty Conditions SAIO is guaranteed for 12 months from the purchase date which is shown on a valid receipt given by the seller The warranty includes free repair or replacement of component parts of the machine that are 16 imperfect because of manufacturing defects Warranty does not cover damage or defects due to Negligence or careless use failure to follow the safety instructions Improper installation or maintenance operated by non qualified personnel Damage caused by transportation or any circumstances which cannot clearly be traced back to defects resulting from errors in the manufacturing process The warranty does not cover any rubber tubing silicone neither the non return valves the filters for the reagents or for washing water because they can be subject to natural wear or natural contamination during normal operations of the machine The warranty does not cover improper installation with not in conformance with the maintenance procedures as specified in this user guide The warranty does not cover any damages caused to th
21. 2 PO O l BE 29 04 2012 Portasiero del 26 03 2012 19 11 54 00 0 C 00 00 OC DL 00 O ee rm AL SEP TT O 0 Picture 6 5 Delete Serum Holder in the Archive 62 7 Archive Control Machine Today s data Archive SAIO machine allows searching in the database both the samples that must be tested and the ones already tested 7 1 Advanced Search File Features 3 Advanced Search EJ Exams Archive Picture 7 1 Advanced Search If clicking Search patient in the archive button a new window will display where the user can enter the time interval for the research and he decide the type of desired search for example by last name first name doctor sector patient type sex or method See Picture 7 2 63 E File Features gt Serum proteins Mi Fractions Picture 7 2 Setting Filters After selecting the desired type the research will start by clicking with the mouse on the button Start Search The result will be displayed in the Archive For more information about curves and patients it is necessary select an item from the Archive 7 2 Exam Archive Print File Features A Advanced Search gt Print Print one or more reports clicking on the right button of the mouse or selecting them through Select Patient button and press the Print Patient see Picture 7 3 In this screen it is possible to print export and block
22. NCELLATION 0000 a A a A OAE 59 bili CANCELLARE NAAA ana A T ee om nina ii ia 60 62 JDERETE SERUM HOLDE P arn a lira rai 62 7 ARCHIVE senenn rca 63 7 1 ADVANCED SEARCH oeoo sOnO AORE AEA RONE anita 63 SAO AA A A EAN 64 7 21 A AD TA n 65 deve QUALITY CONTROL usas li dl ATA 65 8 REGISTRIESa Geri dii rr e A RR rea er RE eNO taaa 67 8 1 LABORATORIO e e fi ORS E UAT MIR ni 67 8 2 A ile i EOE 70 8 3 DOCTOR ss ghi illeciti allea 71 8 4 SE COR G Jalla lle 12 9 VUAELTRN CONROL abllial illa 74 9 1 CONTRATO o e o 74 9 2 LAO A A 76 9 2 1 AR A A RR A INN O Ti 9 3 C E ian 78 9 3 1 PRIMER Rini a ilaria 79 10 DATA EXCHANGE WITH THIRD PARTY APPLICATION 2 cccccccccccccccccccccccccccccccccccccccccces 80 10 1 IMPORT PATEN To ellerre O 80 10 2 RTE DINE eleonora aiar tao aiar tota ina 81 INCLUDE PER IMPORT EXPORT DATA iia 83 KOTE ssi leale dati 86 Table of the Pictures PA CUS dl Upa IN alal 9 Picture 1 Picture 1 Picture 1 Picture 1 Picture 2 Picture 2 Picture 2 Picture 2 Picture 2 Picture 2 Picture 2 Picture 2 Picture 2 Picture 2 Picture 2 Picture 2 Picture 2 2 Connections of probes on the external level i 10 J SalO Completo Plis a E T 12 A sert SAMPLES oa 15 RN E A iena 15 EE E liaison 20 O ride a anale i ceci 21 J Dropdown MENU caian 22 4 First Level Menu Macro Functioning 22 5 Second Level Menu subdivisions Macro Fun
23. SAIO Automatic device for electrophoretic analysis User Manual Clinical Electrophoresis 01 00 00 03 09 2013 P S Elettronica Via Nolana II trav 20 Pompei NA Tel 081 8639760 www pselettronica com email info pselettronica com Table of contents Version I 18 01 2014 LSATO PREAMBLE nile lat al 5 1 1 USER MANDA AND MAINTENANCE o O O e 6 LZ SATE ANOTE laine 7 1 3 TAE E AAA A A A A A A O 8 1 4 SA AA A A rn Eg ae a gee ee ee Tene RT Fe Pe ee 9 1 5 OPERATING PROCEDUPES siria 11 1 6 DRAINING CLEANING AND MAINTENANCE OF THE MACHINE csccecescecceccecccceccecceccecescscssescescscesessescesceses 16 1 7 ANARRANECGONDITION Siziano 16 1 8 PROCEDURES FOR THE DISPOSAL OF THE MACHINE ccececececececece ceci ceci ceci ceci ce ceci ce ceci vece ceci ce ceci ceci ceci cececee 17 1 9 TECHNICAL SPECIFICATIONS 2 a ana 17 2 INTRODUCTION TO SOFTWARE uote 19 2 1 NS A A E 20 3 METHODS aaa A iii 32 3 1 CONFIGURATION PARAMETER nad ida 32 3 2 DEFINHONPRAGTONS abete la 35 3 3 DEFINEFIONCOMMENTS sollecita 38 4 TODAY SDATA ali Cee oN OR PR CE eR UIE OER KORE PORTS EP ACTON OR ELEN PORN TN Oe Et 40 4 1 MANAGMENT SERUM HOLDER dialettica iaia la luz 41 4 2 CHANGE TRACKS AAA A O a a a a aa 44 4 2 1 A Stee eee EE EEE COE A MA AEEA A TREE ENS COE IEEE RR TET EE IEE RS See Teter es ae Tee TT 48 4 3 PRIN ici ida hes dae cee he Ate a recast Bee 52 4 3 1 PRINT RE VIEW pelo rail a aa a A 54 5 CONTROE MACHIN eieaa a A aaa 56 6 CA
24. Tracks Erin 7 archive GE E Archive serum holder today B Sieroproteine a E 27 04 2012 20 46 1 ann as IE mn X ee eS SS NIN CON E 8 Serum holder of 21 04 2012 20 46 07 F Ta Fal Control Machine ll Me E Today s data x Search Exams E Archive Sieroproteine 27 04 2012 20 46 44 Picture 2 8 Managment serum holder f UAfter the insertion of all the data of the first patient see Picture 2 9 click the yellow icon with a floppy disk symbol to save data and confirm by pressing OK 24 Siero proteine 27 04 2012 E i du E a tn x C E DS 27 04 2012 mM 27 04 2012 Picture 2 9 Save Data g Once the data of the patients have been entered and saved the SAIO automatic process can be started by clicking on the key that lights up with yellow icon i see Picture 2 10 NOTE it is possible to insert the data after the start of the automatic process of electrophoresis 25 Sl Sieroproteine Start the machine to the sample reading E r 2704 2012 x Sieroproteine miei J 1 e 7 0 li a n Co i Kelly 56986 1 Serum holder of 2104 2012 20 47 08 00 0 0 00 00 00 0 0 OC Picture 2 10 Start Electrophoresis Process h When running the machine for the samples reading on the screen will appear Machine Control see Picture 2 11 which illustrates the operational functions The image shows the correct placement of objects
25. amples Picture 1 5 Insert serum holder Close the lid of the machine Choose the method and start the electrophoretic process Scanning of the electrophoretic traces At the end of the process of staining and drying the strips take automatically into the reading position in the scanning compartment Afterwards it is possible to proceed with the analysis of the performed readings and make any corrections To include any demographic data and other actions on the charts and on the printers consult the user guide in the section 2 Introduction to Software After turning off the machine it is necessary to empty the serum holder and clean all other components 15 1 6 Draining cleaning and maintenance of the machine Follow these guidelines to perform the unloading and cleaning operations e Turn Off the instrument e Open the cover of the Instrument e Remove the strips holder frame release the membranes e Wash the frames with running water and let them dry e Remove the absorbing buffers from the strips holder and from the dripping support of the frame Throw them into the special waste e Extract the serum Holder and remove the serum from the wells and wash it with distilled water e Put all components in the proper places in the machine e Close the lid of the machine Maintenance operation The Maintenance must be performed only by qualified personnel during repairs remove the plug from the socket Disassemble
26. arch Exams B Department Archive Registries r Fr F F F Configuration Results Restore Layout tab l graphics Al OFF Sn Close Loading Kit Remaining Alarm Kit Patient s Archive Handling In the following screen it is possible to create a database with information regarding the quality control of the examined samples 9 1 Configuration In the following screen it is possible to insert modify and delete references for the quality control of the samples 74 File Features _ Configuration 30 09 2009 Human S 1 Albumin Check Lot Expiration Date 17 1r 30 09 2009 Picture 9 1 Configuration Please find below description for each particular field on this screen Previous control detail and Following control detail keys scroll the sector list Add new control key enters a new sector in the list Remove control key deletes the selected sector from the list Save control key saves the sector selected in the list ge e Clean key resets the parameters of the screen 75 9 2 Results Table 9 Configuration In the following screen it is possible to manage quality control reports in tabular format File Features 3 Configuration S Results graphics Alpha2 Beta Gamma Albumin Albumin Human Human serum 5 fraction Alpha 1 Alpha 2 Beta Gamma Picture 9 2 Results Pl
27. attern e Subtract Baseline to modify the baseline of the graph e Minimum Compute it allows automatic setting of the minimum value on the graph e Export to export the fractions values of the graph to a file CSV or TXT 49 e Gamma Correction it is confidential and may only be changed by the expert staff e Zoom slider zoom the graph for a better consultation enlarges the area in which you placed the mouse on the graph See Picture 4 11 Color Patterns wf Subtract Baseline Minimum Compute Export p Gamma Correction c oomides List rc CO EO St tw Picture 4 11 Zoom Sider e Displaying the grid of the fraction values a banner allows the flow of comments related to the graph e List the possibility to enter a legend inside the chart see Picture 4 12 50 graphic Al Color Patterns w Subtract Baseline Minimum Compute Export Gamma Correction Zoom slider Centered Picture 4 12 Insert Legend e Zoomingof the Image enlarge the entire image of the graph clicking on zoom on the right of the graph window graphic im Color Patterns a Transparent Dotted a Stained w Subtract Baseline Minimum Compute Export Gamma Correction Zoom slider List Picture 4 13 Zooming Of the Image SI 4 3 Print File Features Change Tracks F Print E Management se
28. ced vertically on the left side of the optical reader that is located on the worktable of the machine On the front side of the machine on the right there is the switch button that becomes illuminated when the machine 1s on The machine equipment includes also some tanks containing reagent which load 1t the reagent in the both chambers Imbibition and staining Destaining Migration Chamber There are 6 tanks of reagents Staining Destaining Serumprotein Buffer Hemoglobin Buffer Distilled Water Waste The kit will contain the reagents required for SAIO processes of electrophoresis with all the concerned methods All machines are supplied with sensor cable which connect the machine with the external tanks Pay attention when filling the tanks with the appropriate reagents as indicated on the caps and on the tanks and pay attention when cleaning them In Picture 1 2 there are junctions of tubes connected to the pipes that go to the reagent tanks and liquid discharge On each socket it 1s indicated the predisposed liquid Picture 1 2 Connections of probes on the external level On the same plate there is also a black female connector which must be connected to the male connector which is linked to the sensors of the tanks The male connector can be inserted snugly into the receptacle the female connector then turn it clockwise to ensure a reliable physical and electrical connection 10 Before going on with the elec
29. ctioning 22 Op b 2 ille 22 T mser number Pat a anole a A AE 23 S Managment Serum Noldets as 24 Jae DI RR RI RI A 29 LO Start Elcctrophoresis Processi ies 26 ls control Machina xe 21 Me CMAN STACKS dedo 28 EE PAN camelie 29 Picture 2 14 Brant DOS a ari ii da 30 Picture 2 15s Pint alll selected samples iaa 30 Penre a 31 te E Ei o E E TE E E VE ANE E A E N 33 Picur 3 2 SA pp ee Time a cilea 34 Picture 3 3 Visualizing option of the trace in print 34 Picture S Defimiton Fracioni elsa 35 Picture o gt Group OL ELECO lella 36 Picture 3 6 Normal Values Modification ooccoccnncnnoconoconoconocanocnnocnnconoconoconocanocnnccnoronoconocanocanicnnnona 36 Picie o Save Me updated raciones lee ielia babosa ii 37 Pictite3 8 5ample Cate ia a txt 37 Picture 9 FracuonsColoris race 38 Picture 3 10 Dehnibon Comments allea 39 Peurred NV Oday SS DAA ii od 40 Picture 4 2 Serum Holder LO Sample Aia 41 bicure gt NUMA ella 42 Picture 4 Change Tracks Windows ebano iii 45 Picture N LACA o e ae 45 Picture 6 ModilyiPattern Pacior a sleale 46 Picture 4 8 Export Insert name machine IP address ccccccccncnnnnnnnnnnonnnnnnnnnnnnnnnnnnnnnnnnnnonnnnnnnoos 46 bero hci 48 Picture 4 10 Drop Down Menu Ora riale olanda 49 Picture 41d Color Patent lalla alianti 40 Picture 4 12 ZOOum SIGE iaa iia 50 Peur A V5 2S InSert le Senpai sii de a iii 51 Picture 14 Zoomi
30. e and or malfunctioning do not touch it and contact the manufacturer and or an authorized service center e When using the electrical equipment the following safety measures must be complied Do not touch the appliance with wet or damp hands or feet do not unplug by pulling on power cord To unplug grasp the plug not the power cord do not expose the appliance to the weather rain sun etc when replacing the power cord use only another one with the identical technical characteristics e When the machine is not used anymore it is recommended to unplug the power cord with the hands e To ensure safe operations such as the analysis it is important to have an adequate lighting at a workstation e Eating drinking smoking handling contact lenses applying cosmetics and storing food for human consumption must not be permitted in laboratory areas e Never pipet by mouth Use of the automatic pipet e Do not forget to use DPI which is any equipment that must be worn and kept on in order to protect the body from risks of security laboratory clothing disposable sterile gloves and goggles e Decontaminate work surfaces before and after use and immediately after spills see point 1 6 Draining cleaning and maintenance procedures Avoid unnecessary proximity to high EMF sources e g radiology equipment and CT scanners that may cause interference with the machine thereby affecting its proper functioning 1 3 Unpacking Make
31. e unit by improper use customer neglect or repairs performed by an unauthorized technician In case of repair at an Authorized Service Center all transport risks and any costs will be borne by the purchaser It is in any case excluding the extension of the guarantee as a result of any failure 1 8 Procedures for the disposal of the machine To dispose the machine SAIO is necessary to contact specialized companies as permitted or required by applicable law they will be responsible to recycle and dispose the machine 1 9 Technical Specifications Lipoprotein Electrophoretic Media Dry cellulose acetate plates available in 2 sizes Analytical Capacity of Up to 16 samples per cycle on 2 plates standard plate Analytical Capacity of mini Up to 8 samples per cycle on 2 plates plate Analytical Throughput 8 micro samples in about 30 minutes standard plate 16 micro samples in about 50 minutes Analytical Throughput 4 micro samples in about 30 minutes mini plate 8 micro samples in about 50 minutes Applicator Washing Automatic by circulation of deionized water Migration Chamber Automatic loading of buffer solution Staining and Destaining Automatic loading of solutions Trays Liquid Control By infrared sensor and by time control Interface USB LAN Power Supply 220 240 V ac 50 60 Hz OYE OO es 6m Integrated into the instrument Reading System 8 indipendent channel Bright Source 8 Led ultrabright De
32. ead value of each fraction Picture 4 5 valori Frazioni Frazioni a Rif g dL Int Rif g dL Albumina 0 65 0 00 3 ma 20 EA 0 00 Picture 4 5 Factor Parameter To modify the Factor of the desired fraction it is necessary to click on it with the left button of the mouse and the following window will display asking to insert the number between 0 7 and 1 5 see Picture 4 6 45 Changes factor Pattern 1 insert a number between 0 70 and 1 50 1 00 Picture 4 6 Modify Pattern Factor Please find below description for each particular field on this screen Field Name Description The Previous patient details and the Following patient details buttons scroll the list of patient in the Archive Inserire il nome o l indirizzo IP della macchina dove esportare i risultati dei pazienti Picture 4 7 Export Insert name machine IP address The Export patient result key exports to an external database the results of the reading of a selected patient in the tree control on the left side of the screen By clicking on the button a screen will display to show the machine where to export the selected patient data Usually it is necessary insert the name and the IP address 46 Archive Fraction Values The Add a new fraction group to the current method button will add a new fraction group to the current method of work accordingly to data inserted in Fraction Values The
33. ease find below description for each particular field on this screen Field Name Description Previous control detail and Following control detail keys scroll the list of the doctors The Print key displays the print preview with the results of the quality control reports in tabular format Table 10 Results 9 2 1 Print Preview In this screen it is possible to print the tabulate with the details of the control and the heading of the laboratory which displays in the Personal Data Laboratory screen 2 Anteprima di Stampa LABORATORIO amp NALISI CLINICHE DR POZZETTO 4 5 HE Via della Resistenza n 45 94019 Scafati SA Tel 0015630001 Controllo 16 Lotto 16 Data Scadenza 2105 2009 Dati campioni ricavati dal 15 12 2008 al 1812 2009 Wax Win wedia War CW Albumina 60 00 50 00 55 00 7 20 0 92 Alfal 50 00 40 00 45 00 173 5 61 Alfaz 40 00 30 00 35 00 3 26 2 446 Beta 30 00 20 00 25 00 1 97 0 94 Gamma 0 00 10 00 15 00 6 38 1 52 Albumina 36 50 Atal 2 48 ta 11 96 Beta 13 81 Gamma 34 95 Picture 9 3 Print Preview TI 9 3 Graphs In the following screen it is possible to manage quality control reports in graphical format Fle Features E Configuration El Results graphics 30 09 2009 v H mn v Hunan serum 5 fraction 1 Albumin Picture 9 4 Graphs Please find below description for each particu
34. enance service button The Service Maintenance button opens a drop down menu see Picture 5 2 User services Services Maintenance Clear List Services Open Robotic Arm Picture 5 2 service Maintenance Two options can be selected the first allows the final user to load the cleaning process list see Picture 5 3 User services LOAD SERUM BUFFER IN CHAMBER 1 LOAD SERUM BUFFER IN IMBIBITION LAAN HEMACI RIN RIICCED IN CHARARED 3 1111 Picture 5 3 User services The second option allows you to perform maintenance tasks that are intended only for qualified A password 1s required in order to proceed the operation and as displayed in the following window immissione Password Immettere la password delloperatore Picture 5 4 Password Maintenance 58 6 Cancellation PO Controllo Macchina FU Metodiche PW Dati odierni E Ricerca Esami EJ EJ Archivio SAIO keeps stored the database and authorize the cancellation of samples or whole serum holders from the archive Clicking on the menu Cancellation you access the windows of Delete Exams and Delete Serum holder and proceed the following operations e Clicking on the Search patient in the archive new window will display where the user can enter the time interval for the research and he decide the type of desired search for example by last name first name doctor sector patient type sex or method After selecting
35. ent method To remove comments select the comment line in the list and click the icon i A confirmation message will appear to validate the operation 38 4 _ Comments Bisalbuminemia Inflammation Picture 3 10 Definition Comments in Fractions E Definition Comments 39 4 Today s Data Control Machine Methods Today s data Search Exams El Archive Picture 4 1 Today s Data Window The Today s Data section contains all the information related to the samples acquired during the day It 1s possible to start the process of electrophoresis to access and to modify the results to print out reports Click on the menu Today s data to access the Management Serum holder window Change Traces and Print that allow you to perform respectively the following operations If needed insert a new serum holder using the Add new serum holder to the current method key and enter patient data in each empty slot of serum holder The Previous serum holder details and Next serum holder details keys allow scrolling among the patients of the day If no additional sample has to be inserted in serum holder you can start the SS N electrophoretic process pressing the button 5 Start machine the samples reading It offers a general overview of the samples collected during the day by visualizing the patient data which are modifiable by the user the image of migration and the graph of the
36. ess stops because an error occurred Pressing this button the error is deleted and the process goes on Stop the process key can be clicked when a blocking error occurs P In this case the button stops the process 56 Alarm key lights when the process ends correctly or in case of error Clicking on this button the alarm turns off The Control Machine window appears as shown in the Picture 5 1 On the top there is a power off button that means the machine 1s turned off because the mechanics 1s not supplied Click on button to switch on the machine see Picture 5 1 File Funzionalita i cant comi Livelli Esterni WU Scarico W Colorante W Decolorante W H20 Tampone Livelli Interni Y Colorante Decolorante Y Imbibizione Camera Mi Servizi Utente Valvole Sieroproteine 26 09 2013 21 37 28 Picture 5 1 Control Machine e The picture shows the correct placement of objects inside the machine e The left column shows the status of the inside and outside liquid levels The liquid levels are highlighted in red or in green on the basis on their status e In the center of the field called Migration you can find the values of voltage the current migration and the remaining time of the migration e The State Machine 1s the status of the machine The Services list in order toper form routine maintenance of the machine 57 Maint
37. ethod 4 oh al W for analysis of samples using the yellow button Set the current method of work B 2 This operation updates the second field of the status bar with the current descriptions of the methodology It is also possible to set Configuration Parameters for each method in the default system on the right part of the screen e Defining a group of fractions for the current method both concentration value and absolute value for predefined patients in the system e Managing comments for the current method 3 1 Configuration Parameter 3 Configuration Parameters E Definition Fractions E Definition Comments 32 File Features i 3 Configuration Parameters Definition Fractions 5 Definition Comments E methode i E List mel _N Method a as Wiles MEDIACT y QUADRO PROTEICO ELETTROFORETICO 27 04 2012 20 55 12 In the column List methods on the left side you can see the different types of methods that can be performed Picture 3 1 3 Configuration Parameters f Method 2 Lipoproteins 4 Serum proteins Picture 3 1 List Method To select a method just clicking on it and a yellow check mark icon will light up S Clicking on this icon it will display a message saying that the method was successfully selected and that the program will restart according to the chosen method On the Configuration Parameters screen on the right in front of blue window yo
38. hour of the day Control Machine al Methods Ei Today s data Search Exams E Archive Picture 2 4 First Level Menu Macro Functioning Dashboard Controls Picture 2 5 Second Level Menu subdivisions Macro Functioning Ready Sieroproteine 09 05 2012 10 15 56 Picture 2 6 Status Bar 22 b Before starting the electrophoretic process please ensure that you have selected the appropriate method e If you have selected the appropriate method with the correct setting of the parameters go to step e In the case the method has not been correctly selected or it is the first use of the software or any need to modify the parameters see Chapter 3 Methods and then go to step e c In Current data click on the yellow icon o to add a new sample holder d Digit the number of patients that must be added to serum holder and click OK see Picture 2 7 Enter the number of patients to be added to port serum Min 1 Max 24 Serum holder of 27 04 2012 20 47 08 0 C 0 Ki E Ki 00 OGOTO Picture 2 7 Insert number Patient e When inserting the number of patients from a minimum of 1 to a maximum of 16 on the image of the serum holder on the left you will see the same number of wells 23 becoming red The green well indicates that it is possible to insert the data of the patient by writing in the tab highlighted in yellow see Picture 2 8 File Features Management serum holder Change
39. ione per l operazione di export dati cSend 1 NUM_ PAZIENTI numero pazienti da esportare cSend 2 n RESULTPATIENTINUM_ PAZIENTI strutture dati dei pazienti da esportare unsigned char cSize 4 cSize 0 3 sizeof cSend The export data buffer that will be sent on the communication channel to the third party applications will be the result of the connections of the buffers CSize cSend On top of the buffer will be copied the actual number of bytes that identifies the data import request In the case in which the machine specified in point 1 receives a data buffer with length different from sizeof CSizecSend or on the same length receives an identification code different from EXPORT_PATIENTS must discard the communication If the machine specified in the paragraph 1 receives a buffer data with a length different from sizeof CSizecRequest or if it receives a buffer data with the same length and with identification code different from IMPORT_PATIENTS it must discard the communication In the case in which the machine specified in point 1 receives correctly the results from SAIO then it must respond to the sender with a buffer that indicates the result of the export operation It will respond with the following buffer unsigned char cResult sizeof unsigned long cResult 0 3 1 1 in caso di successo 0 in caso di errore unsigned char cSize 4 cSize 0 3 sizeof cResult The data response buffer
40. ions Create fractions group connected to the method in particular choose the sample category between human and veterinary Picture 3 4 Human Serum from 5 livisor 2434445 CN Fractions Factor Min Max Min Max Min Max Grid 1 Albumin 1 00 520 65 0 520 650 52 0 65 0 No 2 Alphat 1 00 50 20 5 0 2 0 0 N A ri E Kad y deren Alta poy Ani i 2 0 70 13 0 13 0 T0 10 Ratio A G 1 2 10 1 0 2 10 i EL control machine E Methods Today s data E Search Exams Picture 3 4 Definition Fraction In the Picture 3 4 two buttons are highlighted in yellow E they allow you to delete or add a group of fractions SAIO machine usually is delivered and installed with all the parameters of 35 the methods already set correctly but in case you want to make any changes to the methods please follow the below procedure To add a group of fractions click the icon E and enter a new group of fractions from a minimum of 1 to a maximum of 12 and confirm with OK A message will appear asking to enter the name of the new group of fractions see Picture 3 5 Picture 3 5 Group of Fraction To change the normal minimum and maximum values of the fractions select with the mouse the fraction and on the left will appear a checkmark and simultaneously also the values of fraction in the fields above see Picture 3 6 N Fractions Factor Min Max Min Max Min Max Grid Y 1 Albumin 1
41. l the list of serum holder in the Archive The Add key used to insert a new serum holder of the day It is possible to insert only one serum holder before starting the reading process 41 The Sava patient and note serum holder key to save any changes of the patient data or to add a note to the serum holder The Clean button reset the parameters of the screen The Start machine for the sample reading button runs the electrophoresis process and the sample reading Archive This is the tree control on the left that collect the serum holders of Archivio th e d ay P gt Archivio Portasieri 5 Sieroproteine 01 02 2012 A 20 17 5 Serum Holder This control is located in the central part on the left side of the screen it gathers 24 48 slots for the patient samples Portasiera Each slot of the serum holder can assume one of the following f status O O e WHITE empty slot Select with the mouse an empty slot to clear it and to disable the data of the patient Then g 0 f new data can be entered C Enter the number of patients to be added to port serum max 16 a Picture 4 3 Number Patient e YELLOW free slot It is possible to start inserting one or more patients in the serum holder By double clicking on the slot a new screen will appear with the data of the 42 patients that can be added The right side of the screen relating to patient data 1s
42. lar field on this screen Field Name Description Previous control detail and Following control detail keys scroll the list of the doctors Previous control detail and Following control detail keys scroll the list of the doctors The Print button displays the print preview with the results of the quality control reports in tabular format Table 11 Graphs 78 9 3 1 Print Preview In this screen it is possible to print the graph with the details of the control and the heading of the laboratory which displays in the Personal Data Laboratory screen 2 Anteprima di Stampa LABORATORIO ANALISI CLINICHE DR POZZETTO A S N C Via della Resistenza n 45 84018 Scafati SA Tel 0818630001 Controllo 16 Lotto 16 Data Scadenza 21 09 2009 Dati campioni ricavati dal 18 12 2008 al 18 12 2009 4 Beta Walor Atesi 25 00 Intervallo 5 00 1 III CLIL VI OO e i i i ean LILIA RUI 42 LALALA UPN TOT BULIMIA MU lec Picture 9 5 Print Preview Graphs 79 10 Data exchange with third party application The import export operations of the data include information exchange between SAIO applications and third party This exchange happen thorough the protocol TCP IP with the communication channel SOCKET _IMP_EXP_ PORT 3300 10 1 Import Patient The import of patient data from an external database is performed in the feature Current Da
43. ng temido sandalia 51 Picco BW 52 Pictire 4 GF re OS aia licia DZ Picture 4 17 Export Insert name of machine and IP address ccccceeeeeeeeeeeeeeeeeeeees 53 Picture A FS Pont Prev EN sas 54 Picture 3 l Contool Machine enren A E ERT AE paia 57 Picture 2 Serv ice Marmienan6e psisa a a o e 58 Picie S SP aac 58 Picture5 4 Rassword Maintenan a elia 58 Picture 6 l Dele EXIN cla 59 Picture 0 2 Sein Fe Saree T 60 Picture 6 gt Filters Delete Patenti 6l Picture 6 4 Delete Serum Holde esti ido 62 Picture 6 5 Delete Serum Holder in th Archive is 62 Picture 7 Advaneed S archi a esa 63 Pictures 7 2 Sethe Pt iia 64 Picture 7 3 Prat Patent encolado adoptante lied dape caco 65 Picture 8 Picture 8 Picture 8 Picture 8 Picture 9 Picture 9 Picture 9 Picture 9 Picture 9 Chanel o e nl ni a 68 DD ashe eect iach ee Cae eee eee eo 70 FDC VV tiara 71 Li SCO 72 E OMAN ei iii iia 75 Z RO US en E E i i 76 Je PPA PEN a T1 aR E re E EE iaia in 78 De A E E EE E A 79 1 SAIO Preamble SAIO performs the automatic electrophoresis of Serum Proteins Hemoglobins Lipoproteins Isoenzymes not concentrated Urine protein etc and other methods that require incubation step which will be performed in a semi automatic way Additional specific instructions for the methodologies will be describe in the provided kits The equipment of the machine consists of A robotic unit that
44. nsitometer Linearity 0 to 2 8 D O Dedicated for instrument and densitometer management 18 2 Introduction to Software SAIO has a program that exploits all the possibilities that are offered by the intuitive Windows lite In addition to a quantitative reliable and fast electrophoretic analysis SAIO can record all the data in a standard database which 1s easily and rapidly reachable The program is structured with windows that help the user in the sequence of operations to be performed On the first screen you can start clicking with the mouse on the menu choosing the type of operation The program is very flexible both during the performing of the electrophoretic process and after reading the samples During the execution electrophoresis in order to speed up the operations it 1s possible to modify the traces of an earlier scanning and to keep the data archive The scan results are automatically presented on the video and it is possible to correct them and include any comments Within the program there 1s an interactive curves editor which can also modify the trace The editor shows the image of migration the resulting curve and the numerical results with the identification of normality and with the personal data of the patient It 1s possible to change the position of the minimum the names of the fractions and to add comments by simply clicking the mouse on the corresponding button that 1s present on the window Using the Previous
45. performs in a fully automated way all the phases of the process the buffering of strips from 1 to 2 which can be made of cellulose acetate or other kind of supports the drying of the strip the samples deposit migration staining destaining reading with incorporated densitometer 1 1 Index Pi e A A E A built in Computer runs the robotic controllers A software for the machine functioning and management of the program User Manual and Maintenance SAIO was designed and developed in full compliance with all requirements of safety and healthy requirements Please READ CAREFULLY every part of this guide before installing and use the machine Here you will find all the information on how to use the machine and how to keep 1t efficient Keep this booklet with care in case you lose 1t ask for another copy to the manufacturer If you sell or give the machine to another person do not forget to hand this booklet to the new user The manufacturer disclaims any liability for any damage that may be directly or indirectly resulting to persons things and animals occurred as consequence of a wrong observance of the procedures given and explained in this user guide especially the instructions regarding the installation the right use and the maintenance of the machine Safety Notes Unpacking and Installation Operating modes Reagents and samples loading Placement of the membranes of cellulose acetate Running of Machine a Se
46. reen and it is used to insert to modify the data of the patient Table 1 Management Serum Holder 4 2 Change Tracks File Features Management serum holder Change Tracks E Print Archive l il El Tracks Archive today In the window below it is possible to run the following operations changing the patient attaching a comment to the patient changing the image of migration changing the factor changing the graph of the curve and the numerical data of the fractions with the interaction of the mouse with graph see Picture 4 4 44 amp Aries Rel Mar 13 2012 22 20 29 File Features Es Management serum holder E Change Tracks E a Print Print Archive B 4 James Cameron MEE 2 Garibaldi Giuseppe 57 04 2012 3 D Annunzio Gabriele Li om rk Cut p Grafico EN Fat 4 00 Int Rif g d Int Rif g dL 52 0 65 0 3 125 20 Proteine Totali g dL 0 00 Rif 5 00 8 50 1 32 Rif 1 00 2 10 a ee e me To Control Machine E ethos ua Today 5 data E search Exams 5 E archwe Ready Serom aiene 29 04 2012 10 45 19 i Picture 4 4 Change Tracks Window Factor The factor 1s a parameter located in the first column to the right side of the names of the fractions itis used to increase or decrease the numerical r
47. rum holder 3 Picture 4 14 Print Window In this screen you can select and print one or more patients of the day or export or capture patient data File Funzionalit Date Acc Acc Code Method Surname Name Agel Sex Patient Tiente 27 09 2009 sieroproteine _Cognomel Nomet 0 Human Afar EUU gt leroproteme Lognomes homes I IVI Human Picture 4 15 Printing Mode Please find below description for each particular field on this screen 52 Patient List Previous patient details and Following patient details keys scroll the patient list Select patient key in order to select the patients Clicking twice you deselect all the patients of the list Export patient results key exports to an external database the reading results of the selected patients Clicking the key you will see the following screen Picture 4 16 Export Insert name of machine and IP address In order to show where to export the patient data usually you need to enter the name and the IP address of the machine Clean key to reseat the screen parameters The Print patient button displays the print preview with the selected reports Changeable patient data to put in read only the selected patient data The list contains all the patient of the day To print one of the patient of the list click with the right button of the mouse on it Table 3 Print 53 4 3 1 Print
48. rumprotein b Hemglobin 7 Unloading cleaning and maintenance Guarantee Conditions 1 2 Safety Notes The chief of the laboratory must run all the working procedures in the correct way and he must adopt collective and individual protective measures from exposure all the workplaces where biological agents are handled must be marked with the appropriate risk signal and they should be provided adequate sanitation measures in case of accidental propagation and emergency procedures During SAIO use the following measures have to be adopted e SAIO must be used strictly in accordance with the purpose for which it was designed and as specified in this guide The machine must be used only by qualified persons any other type of use is considered improper and therefore dangerous The manufacturer will not assume any responsibility for damages arising out of misuse e Always remove the plug from the power outlet before cleaning or doing any maintenance e Any modification to the machine is extremely dangerous and it implies the end of the guarantee e In case you need an intervention to the electrical system please contact only those persons who are qualified and authorized e To ensure the efficiency of the machine and its proper functioning is essential to follow the manufacturer instructions and call only qualified and authorized persons for the maintenance It should be checked at least twice a year e Switch it off incase of failur
49. s Eeey Desses Doctor Department 0009 Surname Name Specialization Coppola Pasquale Urologo Picture 8 3 Doctor Windows Please find below description for each particular field on this screen Field Name Description Previous doctor detail and Following doctor detail keys scroll the list of the doctors Add new doctor keys enters a new doctor in the list Remove doctor key deletes the selected doctor from the list Save doctor key saves the doctor selected in the list Clean key resets the parameters of the screen 71 Table 7 Doctor 84 Sector In the screen it is possible to manage the sector data File Features Latevtoy psesses oe Department 0000 Departments List Pediatria Picture 4 Sector Please find below description for each particular field on this screen Field Name Description Previous sector detail and Following sector detail keys scroll the sector list Add new sector key enters a new sector in the list Remove sector key deletes the selected sector from the list Save sector key saves the sector selected in the list 72 Clean key resets the parameters of the screen Table 8 Sector 73 9 Quality Control File E f Control Machine d Methods Today s data Se
50. s assigned the fraction Albumin and to the divisor the Globulin You can color the area of fractions of report by clicking on the button which allows two operations see Figure 3 9 e choose a color for the selected fraction on the list 37 e select a grid for the selected fraction on the list A Configuration Parameters E Definition Fractions 5 Definition Comments man uman Serum from 5 e 2434445 bon peek BEE SE SEES S SR N N N HEEE a 0 Bo 3 0 Max Min Max Min Max Grid Colori personalizzati N Fraction np n pai pi pn 1 Albumin 165 00 52 00 65 00 51 00 65 00 No 2 Alphat UG 8 ye og 5 0 2 0 5 0 2 0 5 0 No v3 Alpha a 13 0 7 0 13 0 7 0 13 0 No 4 Beta asta eno pasala 15 0 80 15 0 80 15 0 No 21 0 No 2 10 21 0 12 0 2 10 1 00 12 0 21 0 2 10 Gamma Ratio A G Picture 3 9 Fractions Color 3 3Definition Comments E Configuration Parameters Definition Fractions Definition Comments In Definition Comments 1s possible to insert default comments that can be used in case of pathologies reported by densitometric reading of the traces To enter a new comment select the blank field at the top side and then add to it to the comment list by clicking on the icon A confirmation message will appear in order to validate the successful saving the list of comments of the curr
51. sure the package 1s intact and has no obvious signs of damages otherwise do not install the machine but complaint to the carrier and inform our agent or the distributor Open the box from the top according instructions and pay attention to take out the machine upright Picture 1 1 Picture 1 1 Unpacking Check the following contents of the box e Electrophoresis machine e User Manual e Power Cord e External Sensor Cable e Reagent containers e Starter kit containing absorbent cardboards buffer staining destaining dry or wet acetate strips smart card This kit allows you to carry out electrophoresis tests 14 Installation Put the machine on a table or on a work bench perfectly level Open the Plexiglas cover of the machine remove any sponges or tape Unlock the cart by removing the lock located on the steel frame on the left side it is sufficient to unscrew anticlockwise the pommel on the PVC That lock must be stored it can be useful in case of relocation of the machine it must be reapplied in order to prevent any movement of the cart The instrument contains from to 2 strips holder and 1 sample holder base but the sera applicator will be in another provided container On the left side it is clearly visible an opening with all computer connectors to be linked to external devices On the same panel there is also the master power switch the electric switch and two and 1 6 A fuses A smart card reader is pla
52. t is recommended to run the RESET command following the instructions of the software Control Machine gt users service The delicate software manages every movements of the machine and all its sensors At the end of the electrophoretic process the software acquires the reading data from the densitometer than 1t analyses them and creates a densitometric report with all the data related to the examined sample 1 5 Operating Procedures Loading of Reagent 11 This user guide contains instructions about the loading related to the electrophoresis of serum proteins Other analysis procedures can be found described in the kits with everything needed to run them Picture 1 3 Saio Complete Plan The interior of the unit 1s accessed by opening the cover the power to the electrical circuits and the motors will be consequently automatically interrupted Robotic arm pliers SmartCard reader Positioning of the lid of the migration chamber Densitometer reader Applicator with an automatic wash distilled water Serum holder with 16 basin where inject serum 25 30 micro liters so A A A A Migration chamber with bridges papers holder with bibulous paper 70x20mm 12 that the absorbing the buffer become electrically conductive and keep adequately wet the strip during migration 8 Cover of the migration chamber 9 Positioning of strip drying 10 Imbibition strip tank 11 Tank for staining and destaining of the
53. ta Serumholder management The start of this feature requires the following steps 1 Enter the IP address or the name of the machine which contains the needed patient data 2 Insert the number of patients which data are required It is possible to import data for 24 48 patients because the serum holder provides these number of locations for the samples to be tested 3 The connection with the machine will start via the communication channel SOCKET_IMP_EXP_PORT 3300 4 The request is sent through the composition of the following buffer data unsigned char cRequest 2 cRequest 0 IMPORT_PATIENTS codice di identificazione per l operazione di import dati cRequest 1 NUM_PAZIENTI numero pazienti da importare unsigned char cSize 4 cSize 0 3 sizeof cRequest The buffer of request that will be sent on the communication channelwill be the result of the connections of the buffers CSize cRequest On top of the buffer will be copied the actual number of bytes that identifies the data import request 80 If the machine specified in the paragraph 1 receives a buffer data with a length different from sizeof CSizecRequest or if it receives a buffer data with the same length and with identification code different from IMPORT_PATIENTS it must discard the communication 1 In the positive case the machine specified in paragraph 1 will dial a buffer data with the following format unsigned char cResponse
54. that will be sent to the SAIO communication channel and it will be the result of the connections of the buffers cSize cResult Use the file specified in Appendix A to implement the communication buffer between the SAIO and the third parties 82 A Appendix Include per import export data if Idefined DScanSocketData_h define DScanSocketData_h numero porta del socket per la comunicazione con enti esterni define SOCKET_IMP_EXP_PORT 3300 codice per identificare un operazione di import dei dati define IMPORT_PATIENTS OxBE codice per identificare un operazione di export dei dati define EXPORT_PATIENTS OxCE struttura dati del paziente typedef struct unsigned long m_ImportID ID external assigned to the patient long m_PatientType_Id Tipo paziente O indefinito 1 umano 2 cane 3 gatto 4 cavallo 5 mucca char m_LastName 64 Cognome char m_Name 64 Nome char m_AcceptanceCode 64 Codice Accettazione double m_TotProtein ProteineTotali byte m_Age Eta byte m_Sex Sesso unsigned long m_AcceptanceDate Data Accettazione unsigned long m_BirthDate Data Nascita DATAPATIENT LPDATAPATIENT define SIZE_DATAPATIENT sizeof DATAPATIENT 83 single fraction structure typedefstruct byte m_FractionPos Fraction numeration 1 2 n char m_FractionName 32 Name unsigned long m_FractionStart start position on the graph unsigned long m_FractionEnd
55. the multi language program e From the menu Features you can select the commands Y Reset Layout File to restore size and positions of objects on the screen v Turning off the alarm to turn off the alarm after the completion of an electrophoretic process v Loading Kit to load the kit providing the serial number the unlock code the serial machine inventories 21 v Alarm Kit Remainder to indicate the minimum threshold number of strips Rel Mar 13 2012 22 20 29 File Features ee Control Machine t ker E Change Tracks Ba Methods Arch Today s data j EJ Search Exams t today Archive Registries q Quality Control Li Patien Restore Layout tab 0 Alarm OFF pe dona Surnal Parti Loading Kit Remaining larm Kit TEE ri TAPA Code Picture 2 3 Dropdown menu The second menu is composed of two levels The first level see Picture 2 4 below the application shows all the functions exported by SAIO The second level see Picture 2 5 up to the application shows the subdivisions of feature selected on the first level The program also includes a status Bar see Picture2 6 that provides the user with further information In particular the bar starting from the left 1s divided into three fields e State to indicate a process running on the machine e Selected Method to indicate the reference method during the electrophoretic process of the samples e Date and time date and
56. tion number of slot in the serum holder comment on the paragraph Relation Value A G Relation Value A G Min Relation Value A G Max lunit of measure of the relation A G Proteins value total min Proteins value total max Nength of the graph minimum on the graph fraction of the graph bitmap of the graph bitmap of the strip 85 Notes
57. trical connections it 1s necessary to verify that the main voltage of the network corresponds to the values shown on the rating plate located on the lateral side of the machine Moreover it is Important to check sure that the residential electrical system is adequate for the maximum power absorbed by the unit which 1s indicated on the rating plate The electrical safety of the machine 1s ensured only if it 1s properly connected to an efficient grounding system in compliance with all electrical safety standards The manufacturer cannot be held responsible for any damage caused by the absence of the grounding system Also make sure the connection is made at a plant and outlets of adequate capacity to power absorption In general the use of adapters multiple sockets and or extensions is not recommended If they must be used utilise only those single or multiple adapters and extensions which comply with the current safety regulations However make sure not to exceed the current rating marked on the single adapters and extensions or the maximum power marked on the multiple adapters Plug the power cord into the socket with switch and with fuse tubes and connect it to 220V outlet At this point SAIO machine can be switched on using the switch located on the socket group and then pushing the power button on the front side of the machine Once the machine is switched on it will not move till the start command will be issued After the first switching on i
58. ts or mold inside the bottle will occur it will be necessary to remove the buffer If black spots or mold inside the bottle will occur it will be necessary to remove the destaining The products are intended for professional use in laboratories The left over products the waste resulting from their use and the containers must be removed in accordance with current legislation Protocol Insert the absorbing paper floating in the migration chamber Insert the paper for the drying of the strip that is located on ton one side of the migration chamber Supported strips on Mylar that can be found in the kit can be applied on special strips holder frames It 1s necessary to hook these strips matching the holes with the pins present on the strips holder after having lifted the safety lock making a slight pressure on the central part of strip holder Place the strips holder provided with strips in the chamber containing the electrophoresis buffer If you have to run more than 8 electrophoresis load also other strips on the additional strips holder Serum holder Remove the serum holder frame by sliding outward Pipette from 25 to 30 ml of each sample starting from the basin with number first row and the first basin on the operator side and put the serum in the order indicated see Picture 1 3 Insert the 70 x 14 mm paper buffer into the proper strips holder See Picture 1 4 14 CARTONCINO DEPOSITORE Picture 1 4 Insert s
59. u can make all the possible changes of parameters e Voltage you can vary the tension moving the vertical arrows or you can vary the current deselecting first in the indicator box next to volts 33 e Applied Time sec min you can vary the migration times drying times of the strip times of imbibition staining and destaining see Picture 3 2 Picture 3 2 Applied Time Outside normality Symbol in the printing reports you can decide whether to highlight in red any abnormal results or to use symbols that indicate out of range Unit of measure concentration you can choose the unit of the desired concentration unit Representation you can choose to put in the report the average absolute values normal values of concentration concentration reference of concentration by selecting the respective check box with the mouse Viewing and Intensity color 1 255 Migration it includes the ability to put together the histogram and the real electrophoretic trace of the patient You can change the width and the position of the trace see Picture 3 3 Ratio A G QUADRO PROTEICO ELETTROFORETICO Picture 3 3 Visualizing option of the trace in print e Areas ratio Shows the areas relation and allows the inclusion of any comments e Print it allows you to insert the name of the method on the report before printing 34 3 2Definition Fractions File Features configuration Parameters E Definition Fract
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